应用液体核磁共振波谱技术研究生物大分子的结构、动力学性质和相互作用，研究方向包括：基因转录过程中蛋白质-DNA、蛋白质-蛋白质复合物的组装和调控机制；磷酸化修饰调控蛋白质构象变化和蛋白质-蛋白质间相互作用；致病基因突变诱发蛋白质结构和功能改变的机理等。

   

070302-分析化学
0703Z1-化学生物学
071010-生物化学与分子生物学

蛋白质结构与功能
液体核磁共振

2006-09--2014-06   武汉大学   理学博士
2001-09--2005-06   武汉大学   理学学士

研究生

博士

   

2020-12~现在, 中国科学院精密测量科学与技术创新研究院, 副研究员
2017-10~2020-11,中国科学院精密测量科学与技术创新研究院（原武汉物理与数学研究所）, 助理研究员
2015-07~2017-09,中国科学院武汉物理与数学研究所, 博士后

   

[1] 李颖, 赵淑红, 杨运煌, 胡锐, 朱江, 刘买利. 一种基于植物微纳结构的生化分子细胞递送方法及应用. CN: CN114150022B, 2023-08-22.

[2] 李颖, 赵淑红, 杨运煌, 胡锐, 朱江, 刘买利. 一种基于植物微纳结构的生化分子细胞递送方法及应用. CN: CN114150022A, 2022-03-08.

[3] 杨运煌, 何婷, 胡锐, 朱江, 李双利, 聂瑶. 一种黄曲霉毒素B1纳米抗体的时间分辨荧光免疫层析试剂盒及其制备方法和应用. CN: CN109884008B, 2021-09-10.

[4] 何婷, 杨运煌, 朱江, 胡锐, 聂瑶, 李双利. 一种具有高特异性识别黄曲霉毒素B1的纳米抗体2018AFB-N11及其应用. CN: CN109705215B, 2020-09-04.

[5] 朱江, 杨运煌, 胡锐, 刘买利. 一种杀菌摇床. CN: CN206853583U, 2018-01-09.

   

[1] Zhao, Yin, Chen, Dongjuan, Xu, Zhichen, Li, Tao, Zhu, Jiang, Hu, Rui, Xu, Guoyong, Li, Ying, Yang, Yunhuang, Liu, Maili. Integrating CRISPR-Cas12a into a Microfluidic Dual-Droplet Device Enables Simultaneous Detection of HPV16 and HPV18. ANALYTICAL CHEMISTRY. 2023, http://dx.doi.org/10.1021/acs.analchem.2c05320.
[2] Shan, Yubao, He, Ting, Li, Ying, Zhu, Jiang, Yue, Xiali, Yang, Yunhuang. A Magnetic-Bead-Based Immunoassay with a Newly Developed Monoclonal Antibody for Rapid and Highly Sensitive Detection of Forchlorfenuron. BIOSENSORS-BASEL[J]. 2023, 13(6): http://dx.doi.org/10.3390/bios13060593.
[3] Ru Wang, Xiali Yue, Jiang Zhu, Rui Hu, Ying Li, Yunhuang Yang, Maili Liu. Mutation of two residues converts the ligand-binding domain of RXRα into a uniform monomer without impairing the binding of retinoic acid and cofactors. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2023, 642: 50-56, http://dx.doi.org/10.1016/j.bbrc.2022.12.042.
[4] Zhu, Jiang, Wang, WenShu, Yan, DaWei, Hong, LiWei, Li, TingTing, Gao, Xiang, Yang, YunHuang, Ren, Feng, Lu, YingTang, Yuan, TingTing. CK2 promotes jasmonic acid signaling response by phosphorylating MYC2 in Arabidopsis. NUCLEIC ACIDS RESEARCH[J]. 2023, 51(2): 619-630, http://dx.doi.org/10.1093/nar/gkac1213.
[5] Xu, Zhichen, Chen, Dongjuan, Li, Tao, Yan, Jiayu, Zhu, Jiang, He, Ting, Hu, Rui, Li, Ying, Yang, Yunhuang, Liu, Maili. Microfluidic space coding for multiplexed nucleic acid detection via CRISPR-Cas12a and recombinase polymerase amplification. NATURE COMMUNICATIONS[J]. 2022, 13(1): http://dx.doi.org/10.1038/s41467-022-34086-y.
[6] Wang, Zi, Shan, Yubao, Wang, Ru, Zhou, Heng, Hu, Rui, Li, Ying, Zhu, Jiang, Yang, Yunhuang, Liu, Maili. Structural Insights into the Binding Propensity of Human SHIP2 SH2 to Oncogenic CagA Isoforms from Helicobacter pylori. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES[J]. 2022, 23(19): http://dx.doi.org/10.3390/ijms231911299.
[7] Wang, Biao, He, BangShun, Ruan, XiaoLan, Zhu, Jiang, Hu, Rui, Wang, Jie, Li, Ying, Yang, YunHuang, Liu, MaiLi. An integrated microfluidics platform with high-throughput single-cell cloning array and concentration gradient generator for efficient cancer drug effect screening. MILITARY MEDICAL RESEARCH[J]. 2022, 9(1): http://dx.doi.org/10.1186/s40779-022-00409-9.
[8] Yan, Jiayu, Xu, Zhichen, Zhou, Hu, Li, Tao, Du, Xincheng, Hu, Rui, Zhu, Jiang, Ou, Gaozhi, Li, Ying, Yang, Yunhuang. Integration of CRISPR/Cas12a and Multiplexed RPA for Fast Detection of Gene Doping. ANALYTICAL CHEMISTRY[J]. 2022, 94(47): 16481-16490, http://dx.doi.org/10.1021/acs.analchem.2c04079.
[9] Xiaoling He, Yao Nie, Heng Zhou, Rui Hu, Ying Li, Ting He, Jiang Zhu, Yunhuang Yang, Maili Liu. Structural Insight into the Binding of TGIF1 to SIN3A PAH2 Domain through a C-Terminal Amphipathic Helix. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES[J]. 2021, 22(23): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657803/.
[10] Shan, Yubao, He, Xiaoling, Wang, Zi, Yue, Xiali, Zhu, Jiang, Yang, Yunhuang, Liu, Maili. Structural Investigations on the SH3b Domains of Clostridium perfringens Autolysin through NMR Spectroscopy and Structure Simulation Enlighten the Cell Wall Binding Function. MOLECULES[J]. 2021, 26(18): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470621/.
[11] Heng Zhou, Xiali Yue, Zi Wang, Shuangli Li, Jiang Zhu, Yunhuang Yang, Maili Liu. Expression, purification and characterization of the RhoA-binding domain of human SHIP2 in E.coli. PROTEIN EXPRESSION AND PURIFICATION[J]. 2021, 180: http://dx.doi.org/10.1016/j.pep.2021.105821.
[12] Gong, Yixuan, Li, Shuangli, Li, Ying, Zhu, Jiang, Yang, Yunhuang, Liu, Maili. Mutation of leucine 20 causes a change of local conformation indirectly impairing the DNA binding of SP_0782 from Streptococcus pneumoniae. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2020, 524(1): 103-108, http://dx.doi.org/10.1016/j.bbrc.2020.01.057.
[13] Cai, Cong, Nie, Yao, Gong, Yixuan, Li, Shuangli, Ramelot, Theresa A, Kennedy, Michael A, Yue, Xiali, Zhu, Jiang, Liu, Maili, Yang, Yunhuang. Solution NMR structure of CGL2373, a polyketide cyclase-like protein from Corynebacterium glutamicum. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS[J]. 2020, 88(1): 237-241, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6901738/.
[14] Li, Shuangli, Lu, Guoliang, Fang, Xiang, Ramelot, Theresa A, Kennedy, Michael A, Zhou, Xin, Gong, Peng, Zhang, Xu, Liu, Maili, Zhu, Jiang, Yang, Yunhuang. Structural insight into the length-dependent binding of ssDNA by SP_0782 from Streptococcus pneumoniae, reveals a divergence in the DNA-binding interface of PC4-like proteins. NUCLEIC ACIDS RESEARCH[J]. 2020, 48(1): 432-444, https://www.webofscience.com/wos/woscc/full-record/WOS:000524741700037.
[15] Cai, Cong, Nie, Yao, Yue, Xiali, Zhu, Jiang, Hu, Rui, Liu, Maili, Yang, Yunhuang. Backbone and side chain resonance assignments of the C-terminal domain of human TGIF1. BIOMOLECULAR NMR ASSIGNMENTS[J]. 2019, 13(2): 357-360, https://www.webofscience.com/wos/woscc/full-record/WOS:000483690800018.
[16] Zhu, Wenkai, Li, Ying, Liu, Maili, Zhu, Jiang, Yang, Yunhuang. Uncorrelated Effect of Interdomain Contact on Pin1 Isomerase Activity Reveals Positive Catalytic Cooperativity. JOURNAL OF PHYSICAL CHEMISTRY LETTERS[J]. 2019, 10(6): 1272-1278, https://pubs.acs.org/doi/10.1021/acs.jpclett.9b00052.
[17] Wang, Zi, Nie, Yao, Zhang, Kunxiao, Xu, Henghao, Ramelot, Theresa A, Kennedy, Michael A, Liu, Maili, Zhu, Jiang, Yang, Yunhuang. Solution structure of SHIP2 SH2 domain and its interaction with a phosphotyrosine peptide from c-MET. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS[J]. 2018, 656: 31-37, http://dx.doi.org/10.1016/j.abb.2018.08.012.
[18] Zhu, Jiang, Li, Shuangli, Ramelot, Theresa A, Kennedy, Michael A, Liu, Maili, Yang, Yunhuang. Structural insights into the impact of two holoprosencephaly-related mutations on human TGIF1 homeodomain. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2018, 496(2): 575-581, http://dx.doi.org/10.1016/j.bbrc.2018.01.099.
[19] Li, Shuangli, Hu, Rui, Yao, Haijie, Long, Dong, Luo, Fan, Zhou, Xin, Zhang, Xu, Liu, Maili, Zhu, Jiang, Yang, Yunhuang. Characterization of the interaction interface and conformational dynamics of human TGIF1 homeodomain upon the binding of consensus DNA. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS[J]. 2018, 1866(10): 1021-1028, http://dx.doi.org/10.1016/j.bbapap.2018.07.005.
[20] Zhu, Jiang, Wang, Huapu, Ramelot, Theresa A, Kennedy, Michael A, Hu, Rui, Yue, Xiali, Liu, Maili, Yang, Yunhuang. Solution NMR structure of zinc finger 4 and 5 from human INSM1, an essential regulator of neuroendocrine differentiation. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS[J]. 2017, 85(5): 957-962, http://dx.doi.org/10.1002/prot.25259.

   

（ 1 ） 神经内分泌调控蛋白INSM1锌指结构域的核磁共振结构和功能研究, 主持, 国家级, 2018-01--2020-12
（ 2 ） 核磁共振波谱研究转录抑制复合物Sin3A-TGIF1-Smad2组装的分子机制, 参与, 国家级, 2021-01--2024-12
（ 3 ） 细胞中原子分辨的功能分子构象变化、相互作用和微环境效应的测定, 主持, 国家级, 2020-01--2024-12
（ 4 ） 生物磁共振波谱分析中的原子标记和非标记方法研究, 主持, 国家级, 2018-01--2022-12
（ 5 ） 基于蛋白质液晶化定向排列芯片的RDC核磁样品制备新方法研究, 参与, 国家级, 2018-01--2020-12