纳米孔单分子检测技术及其应用

硕士研究生（具有生物化学/物理，分子生物学，分析化学相关专业背景优先）

071010-生物化学与分子生物学
080300-光学工程
070302-分析化学

纳米孔单分子检测
生物自组装材料

2008-09--2011-06   四川大学   博士研究生

研究生

理学博士

   

2018-01~现在, 中科院重庆绿色智能技术研究院, 研究员
2017-02~2017-07,美国俄亥俄州立大学药学院, 博士后研究员
2012-10~2018-06,美国伊利诺伊理工大学, 高级助理研究员
2011-07~2012-09,江苏大学, 助理研究员

2018-04-10-今,河北省科技厅科技奖励评审专家, 评审专家

重庆英才·青年拔尖人才（首批）

（1） 重庆市北碚区第7届”十大杰出青年“, 市地级, 2022
（2） 重庆市青年科技创新先锋人物, 省级, 2022
（3） 重庆英才·青年拔尖人才, , 省级, 2019

[1] 不同手性 Tau 蛋白及其自组装纤维的检测方法. 2024-01-20.
[2] 一种纳米孔检测丁肝病毒抗原的方法. 2024-04-09.
[3] 特异性结合丁肝病毒抗原的核酸、含有该核酸的探针及其应用. 2024-04-28.
[4] 一种检测心衰标志物的试剂盒和检测方法. 2023-06-07.
[5] 一种纳米孔单分子检测蛋白结构稳态损伤的方法. 2023-05-31.
[6] 王赟姣, 王德强, 王亮, 谢婉谊, 方绍熙, 殷博华, 袁家虎. 一种含有纳米管基纳米通道的芯片及其制备方法和应用. CN: CN115283033A, 2022-11-04.
[7] 田荣, 殷博华, 王德强, 王亮, 何石轩, 方绍熙, 周硕. 一种基于3D DNA walker传感的生物纳米孔检测方法及其在MUC1检测中的应用. CN: CN114994310A, 2022-09-02.
[8] 王亮, 李明翰, 黄文丽, 王德强, 王赟姣. 一种抑制病毒蛋白酶活性的方法. CN: CN114438059A, 2022-05-06.
[9] 王亮, 李明翰, 黄文丽, 王德强, 王赟姣. 一种抑制病毒蛋白酶活性的方法. CN: CN114438059A, 2022-05-06.
[10] 王亮, 肖钇岑, 任光俊, 任鄄胜, 王赟姣, 高方远. 纳米孔在电化学检测植物抗性基因表达多肽中的应用及检测方法. CN: CN113804869A, 2021-12-17.
[11] 王德强, 曾小清, 向阳, 尹雅洁, 王亮, 方绍熙, 谢婉谊, 孔祥东, 马倩云, 马文豪, 曾德林. 一种基于氮化硅（SiNx）固态纳米孔检测HIV-1 P24抗原的方法及装置. CN: CN113219015A, 2021-08-06.
[12] 向阳, 王德强, 曾小清, 尹雅洁, 王亮, 方绍熙, 谢婉谊, 殷博华, 孔祥东, 马倩云, 马文豪, 曾德林. 基于固态纳米孔的HIV-1抗体检测装置及方法. CN: CN113176322A, 2021-07-27.
[13] 向阳, 王德强, 曾小清, 尹雅洁, 王亮, 方绍熙, 谢婉谊, 殷博华, 孔祥东, 马倩云, 马文豪, 曾德林. 基于固态纳米孔的HIV-1抗体+抗原检测装置及方法. CN: CN113176323A, 2021-07-27.
[14] 王亮, 王涵, 王赟姣, 李苇, 刘千山. 金属离子在影响诱导HIV-1的Tat蛋白水解中的应用及其检测方法. CN: CN112505126A, 2021-03-16.
[15] 曾小清, 向阳, 王德强, 王亮, 尹雅洁, 谢婉谊, 方绍熙, 殷博华, 何石轩, 周硕. 一种全血HIV检测装置. CN: CN112782399A, 2021-05-11.
[16] 曾小清, 向阳, 王德强, 王亮, 尹雅洁, 谢婉谊, 方绍熙, 殷博华, 何石轩, 周硕. 一种基于纳米孔的体外HIV蛋白酶检测仪器. CN: CN112345771A, 2021-02-09.
[17] 王赟姣, 王德强, 王亮, 刘畅, 程敏, 周大明, 何石轩, 谢婉谊, 方绍熙, 殷博华, 石彪, 田荣, 袁家虎. 一种纳米晶石墨纳米孔检测芯片及其制备方法和应用. CN: CN112014430A, 2020-12-01.
[18] 王德强, 刘千山, 王亮, 王赟姣, 王涵. 一种基于纳米孔水解反应的免疫球蛋白M检测方法. CN: CN111323469A, 2020-06-23.
[19] 王亮, 王德强, 刘千山, 王赟姣, 王涵. 一种基于纳米通道的抗体单分子检测系统及方法. CN: CN110554079A, 2019-12-10.
[20] 王亮, 王德强, 刘千山, 王赟姣, 王涵. 一种基于纳米通道的抗体单分子检测系统. CN: CN210953911U, 2020-07-07.
[21] 王德强, 方绍熙, 谢婉谊, 王亮, 周硕, 何石轩, 殷博华, 陆文强, 周大明, 翁婷, 王赟姣. 基于纳米孔的体外HIV蛋白酶检测仪器及方法. CN: CN110346579B, 2023-07-07.
[22] 王德强, 方绍熙, 谢婉谊, 王亮, 周硕, 何石轩, 殷博华, 陆文强, 周大明, 翁婷, 王赟姣. 基于纳米孔的体外HIV蛋白酶检测仪器及方法. CN: CN110346579A, 2019-10-18.
[23] 方绍熙, 王德强, 殷博华, 何石轩, 谢婉谊, 周大明, 王赟姣, 唐鹏, 石彪, 梁丽媛, 王亮, 周硕. 一种自动精确定位制备固态纳米孔阵列的系统及方法. CN: CN110436406A, 2019-11-12.
[24] 王德强, 方绍熙, 何石轩, 谢婉谊, 王亮, 周硕, 殷博华, 陆文强, 周大明, 翁婷, 王赟姣. 基于纳米孔的体外HIV蛋白酶检测仪器. CN: CN211374784U, 2020-08-28.
[25] 炭疽病毒蛋白纳米孔电化学检测分析装置及其应用. 2025-05-13.
[26] 一种基于纳米通道的抗体单分子检测系统及方法. 2024-10-22.

近5年发表论文

1.       Jing Li, Yunjiao Wang, Lebing Wang, Yan Wang, Zhirui Zhang, Shilong Liu, Liang Wang*. Hofmeister Effect Matters in Nanopore Stoichiometry. 2025. ACS Materials Letters, DOI: 10.1021/acsmaterialslett.5c00134.

2.       Qianshan Liu, Yulin Ouyang, Yunjiao Wang, Shuo Zhou, Yang Zhan*，Liang Wang*. Multianalyte Nanopore Detection of Alzheimer’s Disease Biomarkers: A Label-Free Platform with Improved Sensitivity and Range. Advanced Healthcare Materials 2025. 2405058.

3.       Wei Li, Jing Li, Lebing Wang, Yan Wang, Zhirui Zhang, Shilong Liu, Bo Gong, Yunjiao Wang*, Liang Wang*. Nanopore Discriminates Watson-Crick and Hoogsteen Hydrogen Bonds in Multiple DNA Contexts. Nano Letters 2025, 25, 4, 1706–1714.

4.       Paolo Actis, Koichi Aoki, Lane A. Baker, Zhifeng Ding, Andrew G. Ewing, Kaiyu X. Fu, J. Justin Gooding, Jun Huang, Frédéric Kanoufi, John J. Kasianowicz, Moonjoo Kim, Christine Kranz, Christian Kuttner, Fei Li, Liang Liu, Wei Liu, Jin Lu, Si-Min Lu, Kim McKelvey, Andrew R. Mount, Hang Ren, Yuanhua Shao, Yasufumi Takahashi, Shuo Tan, Juan Tang, Haiyan Wang, Kang Wang, Liang Wang, Weihua Wang, Fan Xia, Tianyi Xiong, Yi-Lun Ying, Yueming Zhai, Ziwen Zhao and Yige Zhou. Confined nanopore electrochemistry: general discussion. Faraday Discuss., 2025, 257, 88-106.

5.       Paolo Actis, Koichi Aoki, Lane A. Baker, Giada Caniglia, Ning Dang, Zhifeng Ding, Andrew G. Ewing, Kaiyu X. Fu, J. Justin Gooding, Kaoru Hiramoto, Dechen Jiang, Frédéric Kanoufi, John J. Kasianowicz, Christine Kranz, Shuang-Yan Lang, Fei Li, Haoran Li, Liang Liu, Yi-Tao Long, Jin Lu, Si-Min Lu, Kim McKelvey, Andrew R. Mount, Hang Ren, Yuanhua Shao, Mei Shen, Zuzanna S. Siwy, Yasufumi Takahashi, Juan Tang, Haiyan Wang, Jun-Gang Wang, Kang Wang, Liang Wang, Yunong Wang, Fan Xia, Ruo-Chen Xie, Yi-Lun Ying, Guohui Zhang and Ziwen Zhao. Systems nanoelectrochemistry from single entity to ensemble: general discussion. Faraday Discuss., 2025, 257, 399-424.

6.       Paolo Actis, Lane A. Baker, Giada Caniglia, Zhifeng Ding, Andrew G. Ewing, Kaiyu X. Fu, Lachlan F. Gaudin, J. Justin Gooding, Jingshu Hui, Dechen Jiang, Frédéric Kanoufi, John J. Kasianowicz, Moonjoo Kim, Christine Kranz, Christian Kuttner, Yunchao Li, Liang Liu, Si-Min Lu, Wenjun Luo, Kim McKelvey, Andrew R. Mount, Hang Ren, Yuanhua Shao, Mei Shen, Yasufumi Takahashi, Juan Tang, Dengchao Wang, Liang Wang, Wei Wang, Yunong Wang, Ruo-Chen Xie, Yuelin Xie, Yi-Lun Ying, Guohui Zhang, Lingjie Zhang, Ziwen Zhao and Yige Zhou. Scanning electrochemical probe microscopy: general discussion. Faraday Discuss. 2025, 257, 277-302.

7.       Lebing Wang, Shuo Zhou, Yunjiao Wang, Yan Wang, Jing Li, Xiaohan Chen, Daming Zhou, Liyuan Liang, Bohua Yin*, Youwen Zhang* and Liang Wang*. Molecular sandwich-based DNAzyme catalytic reaction towards transducing efficient nanopore electrical detection for antigen proteins. Faraday Discussions, 2025, 257, 60 -72.

8.       Daming Zhou; Yunjiao Wang; Xiaoling Zhang; Shixuan He; Liang Wang; Chaker Tlili; Deqiang Wang. Enhanced current phenomenon in nanocrystalline graphite nanopore. Physics of Fluids. 2024, 36, 072015.

9.       Lingyu Zhao, Yingying Deng, Yunjiao Wang, Shuo Zhou, Bohua Yin, Yunhao Chen, Yan Wang, Jing Li, Lebing Wang, Yong Lin**and Liang Wang*. Nanopore efficiently identifies hepatitis D virus antigens in vitro assay. Materials Today Physics 2024, 46, 101479.

10.    Rongjie Li, Daixin Liu, Meili Ren, Fupeng Qin, Guodong Qi, Dawei Tian, Mingkun Zhang, Wanyi Xie, Liang Wang, Shouqin Lü, Deqiang Wang, Jun Pan, Liyuan Liang. Characterization and modulation of the unimolecular conformation of integrins with nanopore sensors. Chemical Engineering Journal 2024, 492, 152374.

11.    Ligang Chen, Xiaoting Yu, Wei Chen, Fucheng Qiu, Dandan Li, Zhongbo Yang, Songrui Yang, Shengjun Lu, Liang Wang, Shuanglong Feng, Peng Xiu, Mingjie Tang, Huabin Wang*. Nanoscale detection of carbon dots-induced changes in actin skeleton of neural cells. Journal of Colloid and Interface Science. 2024. 668. 293-302.

12.    Pearl Arora, Haiyan Zheng, Sathishkumar Munusamy, Rana Jahani, Liang Wang*, Xiyun Guan. Probe-assisted detection of Fe3+ ions in a multi-functionalized nanopore. Biosensors Bioelectronics. 2024. 251. 116125.

13.    Wei Chen, Yunhao Chen, Yunjiao Wang, Ligang Chen, Shuo Zhou, Dandan Li, Bohua Yin, Zhongbo Yang, Liang Wang,* and Huabin Wang*. Nanoscale observation of heparin-mediated self-assembly of chiral tau enantiomers. Materials Today Physics 2024, 42, 101370.

14.    Rong Tian*, Wenhao Ma, Lue Wang, Wanyi Xie, Yunjiao Wang, Yajie Yin, Ting Weng, Shixuan He, Shaoxi Fang, Liyuan Liang, Liang Wang*, Deqiang Wang*, Jingwei Bai. The combination of DNA nanostructures and materials for highly sensitive electrochemical detection. Bioelectrochemistry 2024, 157, 108651.

15.    Bohua Yina, Peng Tang,* Liang Wang, Wanyi Xie, Xiaohan Chen, Yunjiao Wang, Ting Weng, Rong Tian, Shuo Zhou, Zuobin Wang,* Deqiang Wang*. An Aptamer-Assisted Nanopore Strategy with a Salt Gradient for Direct Protein Sensing. J. Mater. Chem. B, 2023,11, 11064-11072.

16.    Zhong Wang, Fupeng Qin, Ji Wu, Wenhao Ma, Rongjie Li, Ting Weng, Bohua Yin, Liang Wang, Deqiang Wang, Liyuan Liang. Single-molecule recognition of Nucleolin and the interactions with DNA/RNA G-quadruplexes via nanopore decoding. Chemical Engineering Journal. 2023, 473, 145311.

17.    Xiaohan Chen, Shuo Zhou, Yunjiao Wang, Ling Zheng, Sarah Guan, Deqiang Wang, Liang Wang* and Xiyun Guan*. Nanopore single-molecule analysis of biomarkers: Providing possible clues to disease diagnosis. Trends in Analytical Chemistry. 2023. 162. 117060.

18.    Shaoxia Zhang, Yunjiao Wang, Dandan Song, Sarah Guan, Daming Zhou, Linyu Gong, Liyuan Liang, Xiyun Guan* and Liang Wang*. Nanopore discrimination and sensitive plasma detection of multiple natriuretic peptides: The representative biomarker of human heart failure. Biosensors Bioelectronics. 2023. 231. 115299.

19.    Dandan Song, Yunjiao Wang*, Shaoxia Zhang, Lingyu Zhao, Linyu Gong, Liyuan Liang and Liang Wang*. Single-molecule Measurement of Carbonic Anhydrase in Cation Coordinated Environment Using MspA Nanopore. Chinese Journal of Chemistry. 2023. 41. 1341-1347.

20.    Minghan Li, Shanchuan Chen, Yunjiao Wang, Shaoxia Zhang, Dandan Song, Rong Tian, Jia Geng, Liang Wang.* Label-free high-precise nanopore detection of endopeptidase activity of anthrax lethal factor regulated by diverse conditions. Biosensors Bioelectronics. 2023. 219. 114800.

21.    Wei Li, Yunjiao Wang, Yicen Xiao, Minghan Li, Qianshan Liu, Liyuan Liang, Wanyi Xie, Deqiang Wang*, Xiyun Guan*, and Liang Wang*. Simultaneous Dual-Site Identification of 5^mC/8^oG in DNA Triplex Using a Nanopore Sensor. ACS Applied Materials Interfaces. 2022. 14. 32948.

22.    Rong Tian, Bohua Yin, Dejin Liu, Qianshan Liu, Shanchuan Chen, Minghan Li, Liang Wang, Shuo Zhou, Deqiang Wang. Highly sensitive α-Hemolysin nanopore detection of MUC1 based on 3D DNA walker. Analytica Chimica Acta. 2022. 1223.340193.

23.    Liang Wang, Han Wang, Xiaohan Chen, Shuo Zhou, Yunjiao Wang, * and Xiyun Guan*. Chemistry solutions to facilitate nanopore detection and analysis. Biosensors Bioelectronics. 2022. 213. 114448.

24.    Yicen Xiao, Juansheng Ren, Yunjiao Wang, Xiaohan Chen, Shuo Zhou, Minghan Li, Fangyuan Gao, Liyuan Liang, Deqiang Wang, Guangjun Ren* and Liang Wang *. De novo Profiling of Insect-resistant Proteins of Rice via Nanopore Peptide Differentiation. Biosensors Bioelectronics. 2022. 212. 114415.

25.    Ji Wu, Liyuan Liang*, Mingkun Zhang, Rui Zhu, Zhong Wang, Yajie Yin, Bohua Yin, Ting Weng, Shaoxi Fang, Wanyi Xie, Liang Wang, and Deqiang Wang. Single-Molecule Identification of the Conformations of Human C-Reactive Protein and Its Aptamer Complex with Solid-State Nanopores. ACS Appl. Mater. Interfaces 2022, 14, 10, 12077.

26.    Yunjiao Wang, Zequn Wang, Liang Wang, Tianze Tong, Xiaoling Zhang, Shaoxi Fang, Wanyi Xie, Liyuan Liang, Bohua Yin, Jiahu Yuan*, Jin Zhang*, and Deqiang Wang*. Comparison Study on Single Nucleotide Transport Phenomena in Carbon Nanotubes. Nano Letters 2022, 22, 5, 2147.

27.    Han Wang, Wenli Huang, Yunjiao Wang, Wei Li, qianshan liu, Bohua Yin, Liyuan Liang, Deqiang Wang, Xiyun Guan*, Liang Wang*. Enzyme hinders HIV-1 Tat viral transport and real-time measured with nanopores. ACS Sensors. 2021. 6. 3781.

28.    Xiaoqing Zeng, Yang Xiang, Qianshan Liu, Liang Wang, Qianyun Ma, Wenhao Ma, Delin Zeng, Yajie Yin, Deqiang Wang. Nanopore Technology for the Application of Protein Detection. Nanomaterials. 2021. 11. 1942.

29.    Ji Wu, Sen Wang, Liyuan Liang, Chuanqi Zhao, Yajie Yin, Ting Weng, Bohua Yin, Liang Wang, Deqiang Wang. Investigation on the competition of duplex/ G-quadruplex/ i-motif in telomere sequences and c-MYC gene with a solid-state nanopore sensor. Sensors and Actuators B: Chemical. 2021. 348.13072.

30.    Minghan Li, Wei Li, Yicen Xiao, Qianshan Liu, Liyuan Liang, Deqiang Wang, Wenli Huang^* and Liang Wang^*. Nanopore Detects γ-Radiation Inhibited HIV-1 Protease Activity. Biosensors Bioelectronics. 2021. 194. 113602.

31.    Yunjiao Wang, Min Cheng, Liang Wang, Daming Zhou, Shixuan He, Liyuan Liang, Feng Zhang, Chang Liu, Deqiang Wang and Jiahu Yuan. Nanocrystalline Graphite Nanopores for DNA Sensing. Carbon. 2021. 176. 271.

32.    Wanyi Xie, Shixuan He, Shaoxi Fang, Daming Zhou, Yongna Zhang, Liyuan Liang, Liang Wang, and Deqiang Wang. The Raman band shift of suspended graphene impacted by the substrate edge and helium ion irradiation. Nano express. 2021.2.010001.

33.    Qianshan Liu, Yunjiao Wang, Yaqing Liu, Han Wang, Wei Li, Peng Tang, Ting Weng, Shuo Zhou, Liyuan Liang, Jiahu Yuan*, Deqiang Wang*, and Liang Wang*. Reduction Chemistry-Assisted Nanopore Determination Method for Immunoglobulins Isotypes. Nanoscale. 2020. 12. 19711.

34.    Jing Tang, Sen Wang, Ji Wu, Liyuan Liang, Liang Wang, Deqiang Wang. Applications of Photo-Responsive Molecules in Nanopore-based Devices. Chinese Journal of Analytical Chemistry. 2020. 48. 1458.

35.    Yunjiao Wang, Youwen Zhang, Xiaohan Chen, Xiyun Guan*, Liang Wang*. Analytical Strategies with Biological Nanopore: On-pore, off-pore Strategies and application in Biological Fluids. Talanta. 2021. 223. 121684.

36.    Youwen Zhang, Xiaohan Chen, Shaoqing Yuan, Liang Wang*, and Xiyun Guan*. Joint Entropy-assisted Graphene Oxide-based Multiplexing Biosensing Platform for Simultaneous Detection of Multiple Proteases. Analytical Chemistry. 2020. 22. 15042.

37.    Yao Cai, Baocai Zhang, Liyuan Liang, Sen Wang, Lanjun Zhang, Liang Wang, Hongliang Cui, Yihua Zhou, Deqiang Wang. A Solid-State Nanopore-Based Single-Molecule Approach for Label-Free Characterization of Plant Polysaccharides. Plant Communication. 2020. 1. 100106.

38.    Golbarg Mohammadi Roozbahani, Xiaohan Chen, Youwen Zhang, Liang Wang* and Xiyun Guan*. Nanopore Detection of Metal Ions: Current Status and Future Directions. Small Methods. 2020. 4. 2000266.

39.    Sen Wang, Liyuan Liang, Jing Tang, Yao Cai, Chuanqi Zhao, Shaoxi Fang, Huabin Wang, Ting Weng, Liang Wang and Deqiang Wang. Label-free single-molecule identification of telomere G-quadruplexes with a solid-state nanopore sensor. RSC Advances. 2020,10, 27215-27224.

40.    Bohua Yin, Shaoxi Fang, Daming Zhou, Liyuan Liang, Liang Wang, and Zuobin Wang, Deqiang Wang, Jiahu Yuan. Nanopore Fabrication via Transient High Electric Field Controlled Breakdown and Detection of Single RNA Molecules. ACS Applied Bio Materials. 2020. 3. 6368.

41.    Shuo Zhou, Han Wang, Xiaohan Chen, Yunjiao Wang, Daming Zhou, Liyuan Liang, Liang Wang,* Deqiang Wang,* and Xiyun Guan*. Single-Molecule Study on Interactions between Cyclic Nonribosomal Peptides and Protein Nanopore. ACS Appl. Bio Mater. 2020, 3, 554-560.

42.    Liang Wang, Xiaohan Chen, Yunjiao Wang, Shuo Zhou, Deqiang Wang, Xiyun Guan. Nanopore Determination of Nucleic Acids in Whole Blood Based on a Displacement Reaction Strategy. Biophysical J. 2020.118.3.475A. 

   

已指导学生

王涵  硕士研究生  085202-光学工程  

肖钇岑  硕士研究生  085202-光学工程  

李苇  博士研究生  080300-光学工程  

现指导学生

陈云昊  硕士研究生  085400-电子信息  

刘千山  博士研究生  080300-光学工程  

王琰  硕士研究生  085400-电子信息