基本信息
龚睿  男  博导  中国科学院武汉病毒研究所
电子邮件: gongr@wh.iov.cn
通信地址: 湖北省武汉市武昌区小洪山中区44号中科院武汉病毒所
邮政编码: 430071

研究领域

抗体工程学

招生信息

博士、硕士研究生

招生专业
100102-免疫学
100705-微生物与生化药学
071005-微生物学
招生方向
抗体工程与生物药物
抗病毒与肿瘤抗体
病毒与宿主相互作用

教育背景

2002-09--2007-06   武汉大学   研究生博士学位
1998-09--2002-06   武汉大学   本科学士学位
学历

研究生

学位
博士

工作经历

2012-09~现在, 中国科学院武汉病毒研究所, 研究员

2007-09~2012-08,美国国立卫生研究院, 博士后


工作简历
2012-09~现在, 中国科学院武汉病毒研究所, 研究员
2007-09~2012-08,美国国立卫生研究院, 博士后
社会兼职
2017-12-06-2019-12-31,湖北省输血研究所, 理事
2017-11-01-今,国家知识产权局学术委员会, 专利审查技术专家
2015-01-01-今,《antibodies》编委会成员, 编委会成员

教授课程

分子免疫学

专利与奖励

   
奖励信息
(1) Federal Technology Transfer Award, 其他, 2012
(2) Federal Technology Transfer Award, 其他, 2011
专利成果
[1] 龚睿, 张怀东. 一种靶向埃博拉病毒囊膜蛋白的高亲和力单域抗体及其制备方法与应用. CN: CN108191973B, 2021-04-27.

[2] 龚睿, 石剑. 一种中和埃博拉病毒的纳米抗体. CN: CN106188286B, 2019-07-23.

[3] 龚睿, 张怀东. 一种中和新疆出血热病毒的单域抗体. CN: CN106188285B, 2019-07-23.

[4] 龚睿, 李明新, 陈建军. 广谱抗H7流感病毒的中和性人源单克隆抗体及其应用. CN: CN110028578A, 2019-07-19.

[5] 龚睿, 曹广灿, 杨文娟, 石剑, 李璇, 杨春鹏, 张哲. 靶向间皮素的高亲和力C-型单域抗体及其制备方法与应用. 中国: CN108129566A, 2018-06-08.

[6] 龚睿, 肖庚富, 徐婷, 张哲, 陈小波. 一种抗间皮素的纳米抗体及其编码基因和该纳米抗体的用途. 中国: CN103819559A, 2014.05.28.

[7] 肖庚富, 祖向阳, 龚睿, 张哲, 周拯, 王薇, 王宗林, 金卉, 侯政, 刘海滨, 刘洋, 徐婷. 呼吸道合胞病毒F蛋白与Fc的融合蛋白及其用途. 中国: CN103204943A, 2013-07-17.

出版信息

   
发表论文
[1] Hao Wang, Yu Yuan, Mingzhong Xiao, Li Chen, Youyun Zhao, Haiwei Zhang, Pinpin Long, Yana Zhou, Xi Xu, Yanshou Lei, Bihao Wu, Tingyue Diao, Hao Cai, Li Liu, Zuoyu Shao, Jingzhi Wang, Yansen Bai, Kai Wang, Miao Peng, Linlin Liu, Shi Han, Fanghua Mei, Kun Cai, Yake Lei, An Pan, Chaolong Wang, Rui Gong, Xiaodong Li, Tangchun Wu. Dynamics of the SARS-CoV-2 antibody response up to 10 months after infection. CELLULAR & MOLECULAR IMMUNOLOGYnull. 2021, 18(7): 1832-1834, http://dx.doi.org/10.1038/s41423-021-00708-6.
[2] Yuan Zhang, Yanqiu Wei, Yunlong Li, Xuan Wang, Yang Liu, Deyu Tian, Xiaojuan Jia, Rui Gong, Wenjun Liu, Limin Yang. IgY antibodies against Ebola virus possess post-exposure protection in a murine pseudovirus challenge model and excellent thermostability.. PLoS Neglected Tropical Diseases[J]. 2021, 15(3): PMC_JournalArticle.
[3] Xun, Guangjin, Song, Xingpan, Hu, Jie, Zhang, Haiwei, Liu, Lan, Zhang, Zhe, Gong, Rui. Potent Human Single-Domain Antibodies Specific for a Novel Prefusion Epitope of Respiratory Syncytial Virus F Glycoprotein. JOURNAL OF VIROLOGY[J]. 2021, 95(18): [4] Zhao, Shaojuan, Zhang, Huajun, Yang, Xinglou, Zhang, Haiwei, Chen, Ying, Zhan, Yancheng, Zhang, Xiaoqing, Jiang, Rendi, Liu, Meiqin, Liu, Lan, Chen, Li, Tang, Wei, Peng, Cheng, Gao, Xiaoxiao, Zhang, Zhe, Shi, Zhengli, Gong, Rui. Identification of potent human neutralizing antibodies against SARS-CoV-2 implications for development of therapeutics and prophylactics. NATURE COMMUNICATIONS[J]. 2021, 12(1): http://dx.doi.org/10.1038/s41467-021-25153-x.
[5] Wang, Rui, Zhang, Haiwei, Peng, Cheng, Shi, Jian, Zhang, Huajun, Gong, Rui. Identification and Characterization of a Novel Single Domain Antibody Against Ebola Virus. VIROLOGICA SINICA. 2021, 1-11, http://apps.webofknowledge.com/CitedFullRecord.do?product=UA&colName=WOS&SID=5CCFccWmJJRAuMzNPjj&search_mode=CitedFullRecord&isickref=WOS:000705138900001.
[6] Gai, Junwei, Ma, Linlin, Li, Guanghui, Zhu, Min, Qiao, Peng, Li, Xiaofei, Zhang, Haiwei, Zhang, Yanmin, Chen, Yadong, Ji, Weiwei, Zhang, Hao, Cao, Huanhuan, Li, Xionghui, Gong, Rui, Wan, Yakun. A potent neutralizing nanobody against SARS-CoV-2 with inhaled delivery potential. MEDCOMM[J]. 2021, 2(1): 101-113, http://dx.doi.org/10.1002/mco2.60.
[7] Fadlallah, Gendeal M, Ma, Fuying, Zhang, Zherui, Hao, Mengchan, Hu, Juefu, Li, Mingxin, Liu, Haizhou, Liang, Biling, Yao, Yanfeng, Gong, Rui, Zhang, Bo, Liu, Di, Chen, Jianjun. Vaccination with Consensus H7 Elicits Broadly Reactive and Protective Antibodies against Eurasian and North American Lineage H7 Viruses. VACCINES[J]. 2020, 8(1): https://doaj.org/article/b57d4b4cb8c1421189d9c773ac9c7471.
[8] Pan, Xiaoyan, Zhou, Pengfei, Fan, Tiejiong, Wu, Yan, Zhang, Jing, Shi, Xiaoyue, Shang, Weijuan, Fang, Lijuan, Jiang, Xiaming, Shi, Jian, Sun, Yuan, Zhao, Shaojuan, Gong, Rui, Chen, Ze, Xiao, Gengfu. Immunoglobulin fragment F(ab ')(2) against RBD potently neutralizes SARS-CoV-2 in vitro. ANTIVIRAL RESEARCH[J]. 2020, 182: 104868-104868, http://dx.doi.org/10.1016/j.antiviral.2020.104868.
[9] Cao, Guangcan, Gao, Xinyu, Zhan, Yancheng, Wang, Qingguang, Zhang, Zhe, Dimitrov, Dimiter S, Gong, Rui. An engineered human IgG1 CH2 domain with decreased aggregation and nonspecific binding. MABS[J]. 2020, 12(1): https://www.webofscience.com/wos/woscc/full-record/WOS:000612691500001.
[10] Gao, Xinyu, Conard, Alex, Yang, Chunpeng, Zhan, Yancheng, Zeng, Fang, Shi, Jian, Lo, Wei, Dimitrov, Dimiter S, Gong, Rui. Optimization of the C-Terminus of an Autonomous Human IgG1 CH2 Domain for Stability and Aggregation Resistance. MOLECULAR PHARMACEUTICS[J]. 2019, 16(8): 3647-3656, https://www.webofscience.com/wos/woscc/full-record/WOS:000480371700031.
[11] Chunpeng Yang, Rui Gong, Natalia de Val. Development of Neutralizing Antibodies against Zika Virus Based on Its Envelope Protein Structure. 中国病毒学:英文版null. 2019, 34(2): 168-174, http://lib.cqvip.com/Qikan/Article/Detail?id=7002144566.
[12] Li, Mingxin, Chen, Li, Wang, Qingguang, Hao, Mengchan, Zhang, Xiaoqing, Liu, Linlin, Yu, Xiao, Yang, Chunpeng, Xu, Junqiang, Chen, Jianjun, Gong, Rui. A cross-reactive human monoclonal antibody targets the conserved H7 antigenic site A from fifth wave H7N9-infected humans. ANTIVIRAL RESEARCH[J]. 2019, 170: 104556-, http://dx.doi.org/10.1016/j.antiviral.2019.104556.
[13] Yang, Chunpeng, Gong, Rui, de Val, Natalia. Development of Neutralizing Antibodies against Zika Virus Based on Its Envelope Protein Structure. VIROLOGICA SINICAnull. 2019, 34(2): 168-174, http://lib.cqvip.com/Qikan/Article/Detail?id=7002144566.
[14] Yang, Chunpeng, Zeng, Fang, Gao, Xinyu, Zhao, Shaojuan, Li, Xuan, Liu, Sheng, Li, Na, Deng, Chenglin, Zhang, Bo, Gong, Rui. Characterization of two engineered dimeric Zika virus envelope proteins as immunogens for neutralizing antibody selection and vaccine design. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2019, 294(27): 10638-10648, http://dx.doi.org/10.1074/jbc.RA119.007443.
[15] Rui Gong. Characterization of two engineered dimeric Zika virus envelope proteins as immunogen for neutralizing antibody selection and vaccine design. J Biol Chem. 2019, [16] Zeng, Fang, Yang, Chunpeng, Gao, Xinyu, Li, Xuan, Zhang, Zhe, Gong, Rui. Comprehensive elucidation of the structural and functional roles of engineered disulfide bonds in antibody Fc fragment. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2018, 293(49): 19127-19135, http://dx.doi.org/10.1074/jbc.RA118.005367.
[17] Lei, Cheng, Gong, Rui, Ying, Tianlei. Antibody Fc Engineering: Towards Better Therapeutics. FRONTIERS IN IMMUNOLOGYnull. 2018, 9: http://202.127.146.157/handle/2RYDP1HH/6003.
[18] Yang, Chunpeng, Gao, Xinyu, Gong, Rui. Engineering of Fc Fragments with Optimized Physicochemical Properties Implying Improvement of Clinical Potentials for Fc-Based Therapeutics. FRONTIERS IN IMMUNOLOGYnull. 2018, 8: http://202.127.146.157/handle/2RYDP1HH/4499.
[19] Wang, Ying, Shan, Yaming, Gao, Xinyu, Gong, Rui, Zheng, Jun, Zhang, Xiaohua Douglas, Zhao, Qi. Screening and expressing HIV-1 specific antibody fragments in Saccharomyces cerevisiae. MOLECULAR IMMUNOLOGY[J]. 2018, 103: 279-285, http://www.corc.org.cn/handle/1471x/2373226.
[20] 刘欢, 张怀东, 陈晓晖, 龚睿. 克里米亚-刚果出血热病毒新型治疗性抗体研制展望. 中国感染控制杂志. 2018, 17(9): 838-842, http://lib.cqvip.com/Qikan/Article/Detail?id=7000786499.
[21] Sun, Yaping, Zhang, Huaidong, Shi, Jian, Zhang, Zhe, Gong, Rui. Identification of a Novel Inhibitor against Middle East Respiratory Syndrome Coronavirus. VIRUSES-BASEL[J]. 2017, 9(9): http://www.corc.org.cn/handle/1471x/2373387.
[22] Li, Dezhi, Gong, Rui, Zheng, Jun, Chen, Xihai, Dimitrov, Dimiter S, Zhao, Qi. Engineered antibody CH2 domains binding to nucleolin: Isolation, characterization and improvement of aggregation. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2017, 485(2): 446-453, http://dx.doi.org/10.1016/j.bbrc.2017.02.058.
[23] Yaping Sun, Huaidong Zhang, Jian Shi, Zhe Zhang, Rui Gong. Identification of a Novel Inhibitor against Middle East Respiratory Syndrome Coronavirus. Viruses[J]. 2017, 9(9): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618021/.
[24] Chen, Xiaobo, Zeng, Fang, Huang, Tao, Cheng, Liang, Liu, Huan, Gong, Rui. Optimization on Fc for Improvement of Stability and Aggregation Resistance. CURRENT PHARMACEUTICAL BIOTECHNOLOGYnull. 2016, 17(15): 1353-1359, http://www.corc.org.cn/handle/1471x/2373483.
[25] Gong, Rui. Fc-related Antibody Engineering. CURRENT PHARMACEUTICAL BIOTECHNOLOGYnull. 2016, 17(15): 1296-1297, https://www.webofscience.com/wos/woscc/full-record/WOS:000390351700001.
[26] Ying, Tianlei, Wang, Yanping, Feng, Yang, Prabakaran, Ponraj, Gong, Rui, Wang, Lili, Crowder, Karalyne, Dimitrov, Dimiter S. Engineered antibody domains with significantly increased transcytosis and half-life in macaques mediated by FcRn. MABS[J]. 2015, 7(5): 922-930, https://www.webofscience.com/wos/woscc/full-record/WOS:000360218900013.
[27] Zu, Xiangyang, Liu, Yang, Wang, Shaobo, Jin, Rui, Zhou, Zheng, Liu, Haibin, Gong, Rui, Xiao, Gengfu, Wang, Wei. Peptide inhibitor of Japanese encephalitis virus infection targeting envelope protein domain III. ANTIVIRAL RESEARCH[J]. 2014, 104: 7-14, http://dx.doi.org/10.1016/j.antiviral.2014.01.011.
[28] Ying, Tianlei, Gong, Rui, Ju, Tina W, Prabakaran, Ponraj, Dimitrov, Dimiter S. Engineered Fc based antibody domains and fragments as novel scaffolds. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICSnull. 2014, 1844(11): 1977-1982, http://dx.doi.org/10.1016/j.bbapap.2014.04.018.
[29] Tianlei Ying, Rui Gong, Tina W. Ju, Ponraj Prabakaran, Dimiter S. Dimitrov. Engineered Fc based antibody domains and fragments as novel scaffolds. BBA - Proteins and Proteomics. 2014, 1844(11): 1977-1982, http://dx.doi.org/10.1016/j.bbapap.2014.04.018.
[30] 肖葭杨, 龚睿. 茶叶抗病毒作用研究的新进展. 氨基酸和生物资源. 2014, http://lib.cqvip.com/Qikan/Article/Detail?id=1003250558.
[31] 郭露玲. Real-time quantitative PCR assay for rapid detection of murine virus contamination in bioproducts. 中国病毒学(英文版). 2014, 193-195, http://lib.cqvip.com/Qikan/Article/Detail?id=50127212.
[32] Gong, Rui, Wang, Yanping, Ying, Tianlei, Peng, Yang, Streaker, Emily, Prabakaran, Ponraj, Dimitrov, Dimiter S. N-Terminal Truncation of an Isolated Human IgG1 CH2 Domain Significantly Increases Its Stability and Aggregation Resistance. MOLECULAR PHARMACEUTICS[J]. 2013, 10(7): 2642-2652, https://www.webofscience.com/wos/woscc/full-record/WOS:000321472000013.
[33] Rui Gong. Engineered antibody variable and constant domains as therapeutic candidates. Pharm Pat Anal. 2013, [34] Zhongyu Zhu, Ponraj Prabakaran, Weizao Chen, Christopher C Broder, Rui Gong, Dimiter S Dimitrov. Human monoclonal antibodies as candidate therapeutics against emerging viruses and HIV-1. Virologica Sinica. 2013, 28(2): 71-80, http://lib.cqvip.com/Qikan/Article/Detail?id=45401737.
[35] Zhu Zhongyu, Prabakaran Ponraj, Chen Weizao, Broder Christopher C, Gong Rui, Dimitrov Dimiter S. Review Human Monoclonal Antibodies as Candidate Therapeutics Against Emerging Viruses and HIV-1. Virologica Sinica[J]. 2013, 28(2): 71-80, [36] Gong, Rui, Chen, Weizao, Dimitrov, Dimiter S. Candidate Antibody-Based Therapeutics Against HIV-1. BIODRUGSnull. 2012, 26(3): 143-162, http://dx.doi.org/10.2165/11631400-000000000-00000.
[37] Ponraj ePrabakaran, Zhongyu eZhu, Weizao eChen, Rui eGong, Yang eFeng, Emily eStreaker, Dimiter S Dimitrov. Origin, diversity and maturation of human antiviral antibodies analyzed by high-throughput sequencing. Frontiers in Microbiology[J]. 2012, 3: https://doaj.org/article/0ade4200150743f3a3f2fcaa9472d5b4.
[38] Ying, Tianlei, Chen, Weizao, Gong, Rui, Feng, Yang, Dimitrov, Dimiter S. Soluble Monomeric IgG1 Fc. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2012, 287(23): 19399-19408, http://dx.doi.org/10.1074/jbc.M112.368647.
[39] Rui Gong. Bispecific Engineered Antibody Domains (Nanoantibodies) That Interact Noncompetitively with an HIV-1 Neutralizing Epitope and FcRnBispecific Engineered Antibody Domains (Nanoantibodies) That Interact Noncompetitively with an HIV-1 Neutralizing Epitope and FcRn. PLoS One. 2012, [40] Gehlsen, Kurt R, Gong, Rui, Bramhill, Dave, Wiersma, David A, Kirkpatrick, Shaun A, Wang, Yangping, Feng, Yang, Dimitrov, Dimiter S. Pharmacokinetics of engineered human monomeric and dimeric CH2 domains. MABS[J]. 2012, 4(4): 466-474, https://www.webofscience.com/wos/woscc/full-record/WOS:000305961800004.
[41] Gong, Rui, Wang, Yanping, Feng, Yang, Zhao, Qi, Dimitrov, Dimiter S. Shortened Engineered Human Antibody CH2 Domains INCREASED STABILITY AND BINDING TO THE HUMAN NEONATAL Fc RECEPTOR. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2011, 286(31): 27288-27293, http://dx.doi.org/10.1074/jbc.M111.254219.
[42] Chen, Weizao, Feng, Yang, Gong, Rui, Zhu, Zhongyu, Wang, Yanping, Zhao, Qi, Dimitrov, Dimiter S. Engineered Single Human CD4 Domains as Potent HIV-1 Inhibitors and Components of Vaccine Immunogens. JOURNAL OF VIROLOGY[J]. 2011, 85(18): 9395-9405, https://www.webofscience.com/wos/woscc/full-record/WOS:000293956400014.
[43] Feng, Yang, Gong, Rui, Dimitrov, Dimiter S. Design, expression and characterization of a soluble single-chain functional human neonatal Fc receptor. PROTEIN EXPRESSION AND PURIFICATION[J]. 2011, 79(1): 66-71, http://dx.doi.org/10.1016/j.pep.2011.03.012.
[44] Gong, Rui, Vu, Bang K, Feng, Yang, Prieto, DaRue A, Dyba, Marzena A, Walsh, Joseph D, Prabakaran, Ponraj, Veenstra, Timothy D, Tarasov, Sergey G, Ishima, Rieko, Dimitrov, Dimiter S. Engineered Human Antibody Constant Domains with Increased Stability. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2009, 284(21): 14203-14210, http://dx.doi.org/10.1074/jbc.M900769200.

科研活动

   
科研项目
( 1 ) 靶向间皮素关键位点的纳米抗体研发, 主持, 部委级, 2016-01--2017-12
( 2 ) 烈性病原抗体类生物制剂研制, 参与, 部委级, 2016-01--2018-12
( 3 ) 重要人类病毒黏膜疫苗和治疗性抗体研发, 主持, 市地级, 2016-11--2020-12
( 4 ) 靶向间皮素的新型C-型单域抗体筛选及其抗肿瘤作用机制研究, 主持, 国家级, 2019-01--2022-12
参与会议
(1)C-型单域抗体候选药物开发   2019 生物治疗产业大会   2019-03-24
(2)Development of C-based single domain antibodies as therapeutic candidates against infectious diseases   2018-12-02
(3)Development of Nanoantibodies against Viral Infection and Cancer   2016-09-20
(4)Optimization on Antibody CH2 Domain for Reduction of Aggregation: Implication for New Scaffold Design   Rui Gong   2014-04-01
(5)Engineered Antibody Constant Domains (Nanoantibodies): Implications for Development of Novel Candidate Therapeutics   Rui Gong   2013-05-28

指导学生

已指导学生

陈小波  硕士研究生  085238-生物工程  

徐婷  硕士研究生  085238-生物工程  

杨文娟  硕士研究生  085238-生物工程  

孙雅萍  博士研究生  071010-生物化学与分子生物学  

曾芳  硕士研究生  100102-免疫学  

曹广灿  博士研究生  100102-免疫学  

李明新  博士研究生  100102-免疫学  

现指导学生

高新宇  博士研究生  100102-免疫学  

王睿  博士研究生  071005-微生物学  

寻广谨  博士研究生  100102-免疫学  

詹焱程  博士研究生  071010-生物化学与分子生物学  

杨春鹏  博士研究生  100102-免疫学  

赵少娟  博士研究生  100102-免疫学  

王庆光  硕士研究生  085238-生物工程  

张晓晴  硕士研究生  071010-生物化学与分子生物学  

易雨晴  硕士研究生  071010-生物化学与分子生物学  

杨凡  硕士研究生  071005-微生物学  

李默轩  硕士研究生  071005-微生物学  

陈荔  博士研究生  100102-免疫学