Biophysics, Biochemistry and Molecular Biology

Chemical Biology, Chemical Physics

Structure and Function of Biological Macromolecules

Nuclear Magnetic Resonance

​

1998-08 -- 2003-08  University of Maryland   Ph.D.

1994-09 -- 1998-06  Zhejiang University        Bachelor's degree

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2003 — 2007  Postdoc, National Institutes of Health

2007 — 2010  Assistant Professor, University of Missouri

2009 — now   Principal Investigator, Chinese Academy of Sciences

2013 — now   Adjunct Professor, Zhejiang University School of Medicine

2014 — now   Director, CAS Key Laboratory of Magnetic Resonance in Biological Systems

46. Zhang JR, Liu N, Chacho RA, Gong Z, Liu Z, Qin WM, Tang C, Tang Y, Zhou JH. (2016).  Structural basis of nonribosomal pepetide macrocyclization in fungi. Nat Chem Biol, doi: 10.1038/nchembio.2202.

45. Wang X, Feng J, Xue Y, Guan ZY, Zhang DL, Liu Z, Gong Z, Wang Q, Huang JB, Tang C, Zou TT, Yin P. (2016) Structural basis of N6-adenosine methylation by the METTL3–METTL14 complex. Nature, 534,575-578 

44. Fang J, Cheng J, Wang J, Zhang Q, Liu M, Gong R, Wang P, Zhang X, Feng Y, Lan W, Gong Z, Tang C, Wong J, Yang H, Cao C, Xu Y. (2016) Hemi-methylated DNA opens a closed conformation of UHRF1 to facilitate its histone recognition. Nature Communications, doi:10.1038/ncomms11197.

43. Jiang WX, Dong X, Jiang J, Yang YH, Yang J, Lu Yb, Fang SH, Wei EQ, Tang C, and Zhang WP. (2016) Specific cell surface labeling of GPCRs using split GFP. Sci.Rep. , doi: 10.1038/srep20568. 

42. Liu Z and Tang C. (2016) Ensemble structure description of Lys63-linked diubiquitin. Data in Brief, doi:10.1016/j.dib.2016.02.003. 

41. Gong Z, Ding YH, Dong X, Liu N, Zhang EE, Dong MQ and Tang C. (2016) Visualizing the ensemble structures of protein complexes using chemical cross-linking coupled with mass spectrometry. Biophys.Rep. , doi:10.1007/s41048-015-0015-y.

40. Liu Z, Gong Z, Dong X and Tang C. (2016) Transient protein-protein interactions visualized by solution NMR. BBA Proteins and Proteomics, 1864, 115-122. (invited review)

39. Liu Z, Gong Z, Jiang WX, Yang J, Zhu WK, Guo DC, Zhang WP, Liu ML and Tang C. (2015) Lys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition. eLife,4:e05767. 

38. Zhang XQ, Lu JT, Jiang WX, Lu YB, Wu M, Wei EQ, Zhang WP and Tang C. (2015) NAMPT inhibitor and metabolite protect mouse brain from cryoinjury through distinct mechanisms. Neuroscience, 291, 230-40. 

37. Gong Z, Charles DS and Tang C. (2015) Conjoined use of EM and NMR in RNA structure refinement. Plos One, 10, e0120445.

36. Xing Q, Huang P, Yang J, Sun JQ, Gong Z, Dong X, Guo DC, Chen SM, Yang YH, Wang Y, Yang MH, Yi M, Ding YM, Liu ML, Zhang WP and Tang C. (2014) Visualizing an ultra-weak protein-protein interaction in phosphorylation signaling. Angew Chem Int Ed ,53, 11501-11505. (back cover) 

35. Wang Y, Tang C, Wang EK and Wang J. (2014) Polyubiquitin chain linkage topology selects the functions from the underlying binding landscape. Plos Comput Biol, 10. 

34. Zhang Z, Zhang TL, Wang SS, Gong Z, Tang C, Chen JY and Ding JP. (2014) Molecular mechanism for Rabex-5 GEF activation by Rabaptin-5. eLife, doi:10.7554/eLife.02687. 

33. Rathinavelan T, Lara-Tejero M, Lefebre M, Chatterjee S, McShan AC, Guo DC, Tang C, Galan JE and De Guzman RN. (2014) NMR model of prgI-sipD interaction and its implications in the needle-tip assembly of the salmonella type III secretion system. J Mol Biol 426, 2958-2969.

32. Liu Z, Gong Z, Guo DC, Zhang WP and Tang C. (2014) Subtle dynamics of holo glutamine binding protein revealed with a rigid paramagnetic probe. Biochemistry-Us, 53, 1403-1409.

31. Gu XH, Gong Z, Guo DC, Zhang WP and Tang C. (2014) A decadentate Gd(III)-coordinating paramagnetic cosolvent for protein relaxation enhancement measurement. J Biomol NMR, 58, 149-154.

30. Coffman K, Yang B, Lu J, Tetlow AL, Pelliccio E, Lu S, Guo DC, Tang C, Dong MQ and Tamanoi F. (2014) Characterization of the Raptor/ 4E-BP1 Interaction by chemical cross- linking coupled with mass spectrometry analysis. J Biol Chem, 289, 4723-4734.

29. Zhao B, Zhang M, Han X, Zhang XY, Xing Q, Dong X, Shi QJ, Huang P, Lu YB, Wei EQ, Xia Q, Zhang WP and Tang C. (2013) Cerebral ischemia is exacerbated by extracellular nicotinamide phosphoribosyltransferase via a non-enzymatic mechanism. Plos One, 8.

28. Li CG, Tang C and Liu ML. (2013) Protein dynamics elucidated by NMR technique. Protein Cell, 4, 726-730.

27. Jiang B, Luo F, Ding YM, Sun P, Zhang X, Jiang L, Li CG, Mao XA, Yang DW, Tang C and Liu ML. (2013) NASR: an effective approach for simultaneous noise and artifact suppression in NMR spectroscopy. Anal Chem, 85, 2523-2528.

26. Wang Y, Tang C, Wang EK and Wang J. (2012) Exploration of multi-state conformational dynamics and underlying global functional landscape of maltose binding protein. Plos Comput Biol,8. [pubmed]

25. Liu Z, Zhang WP, Xing Q, Ren XF, Liu ML and Tang C. (2012) Noncovalent dimerization of ubiquitin. Angew Chem Int Ed, 51, 469-472.

24. Liu Z, Tang C (2011) . Paramagnetic relaxation enhancement --- A tool for visualizing transientprotein structures. Chinese J Mag Res, 28, 301-316.

23. Rathinavelan T, Tang C and De Guzman RN. (2011) Characterization of the interaction between the salmonella type III secretion system tip protein sipD and the needle protein prgI by paramagnetic relaxation enhancement. J Biol Chem, 286, 4922-4930.

22. Yu DM, Volkov AN and Tang C. (2009) Characterizing dynamic protein-protein interactions using differentially scaled paramagnetic relaxation enhancement. J Am Chem Soc, 131, 17291-17297.

21. Tang C, Louis JM, Aniana A, Suh JY and Clore GM. (2008) Visualizing transient events in amino-terminal autoprocessing of HIV-1 protease. Nature, 455, 693-696.

20. Tang C, Ghirlando R and Clore GM. (2008) Visualization of transient ultra-weak protein self-association in solution using paramagnetic relaxation enhancement. J Am Chem Soc, 130, 4048-4056. 

19. Kim YC, Tang C, Clore GM and Hummer G. (2008) Replica exchange simulations of transient encounter complexes in protein-protein association. P Natl Acad Sci USA, 105, 12855-12860.

18. Tang C, Schwieters CD and Clore GM. (2007) Open-to-closed transition in apo maltose-binding protein observed by paramagnetic NMR. Nature 449, 1078-1082. 

17. Clore GM, Tang C and Iwahara J. (2007) Elucidating transient macromolecular interactions using paramagnetic relaxation enhancement. Curr Opin Struc Biol, 17, 603-616. 

16. Iwahara J, Tang C and Marius Clore G. (2007) Practical aspects of (1)H transverse paramagnetic relaxation enhancement measurements on macromolecules. Journal of magnetic resonance, 184, 185-195.

15. Kelly BN, Kyere S, Kinde I, Tang C, Howard BR, Robinson H, Sundquist WI, Summers MF and Hill CP. (2007) Structure of the antiviral assembly inhibitor CAP-1 complex with the HIV-1 CA protein. J Mol Biol, 373, 355-366.

14. Suh JY, Tang C and Clore GM. (2007) Role of electrostatic interactions in transient encounter complexes in protein-protein association investigated by paramagnetic relaxation enhancement. J Am Chem Soc, 129, 12954-12955.

13. Tang C, Iwahara J and Clore GM. (2006) Visualization of transient encounter complexes in protein-protein association. Nature, 444, 383-386.

12. Tang C and Clore GM. (2006) A simple and reliable approach to docking protein-protein complexes from very sparse NOE-derived intermolecular distance restraints. J Biomol NMR, 36, 37-44.

11. Tang C, Williams DC, Ghirlando R and Clore GM. (2005) Solution structure of enzyme IIA(Chitobiose) from the N,N '-diacetylchitobiose branch of the Escherichia coli phosphotransferase system. J Biol Chem 280, 11770-11780.

10. Suh JY, Tang C, Cai ML and Clore GM. (2005) Visualization of the phosphorylated active site loop of the cytoplasmic B domain of the mannitol transporter IIMannitol of the Escherichia coli phosphotransferase system by NMR spectroscopy and residual dipolar couplings. J Mol Biol 353, 1129-1136.

9. Tang C, Iwahara J and Clore G. (2005) Accurate determination of leucine and valine side-chain conformations using U-[N-15/C-13/H-2]/[H-1-(methine/methyl)-Leu/Val] isotope labeling, NOE pattern recognition, and methine C gamma-H gamma/C beta-H beta residual dipolar couplings: application to the 34-kDa enzyme IIA (Chitobiose). J Biomol NMR 33, 105-121.

8. Tang C, Loeliger E, Luncsford P, Kinde I, Beckett, D and Summers MF. (2004) Entropic switch regulates myristate exposure in the HIV-1 matrix protein. P Natl Acad Sci USA 101, 517-522.

7. Tang C, Loeliger E, Kinde I, Kyere S, Mayo K, Barklis E, Sun YN, Huang MJ and Summers MF. (2003) Antiviral inhibition of the HIV-1 capsid protein. J Mol Biol 327, 1013-1020.

6. Gatanaga H, Suzuki Y, Tsang H, Yoshimura K, Kavlick MF, Nagashima K, Gorelick R. J, Mardy S, Tang C, Summers MF and Mitsuya H. (2002) Amino acid substitutions in Gag protein at non-cleavage sites are indispensable for the development of a high multitude of HIV-1 resistance against protease inhibitors. J Biol Chem 277, 5952-5961.

5. Tang C, Ndassa Y and Summers MF. (2002) Structure of the N-terminal 283-residue fragment of the immature HIV-1 Gag polyprotein. Nat Struct Biol 9, 537-543. 

4. Lin J, Blake M, Tang C, Zimmer D, Rustandi RR, Weber DJ and Carrier F. (2001) Inhibition of p53 transcriptional activity by the S100B calcium-binding protein. J Biol Chem, 276, 35037-35041. 

3. Gu T, Tang C and Xu ZD. (1999) Synthesis of C-60 nitroxide derivatives. Chinese Chem Lett, 10,95-96. 

2. Gu T, Tang, C and Xu ZD. (1999) Two methods to synthesize C-60 nitroxide derivatives. Fullerence Sci Techn, 7,297-303.

1. Baldisseri DM, Rustandi RR, Zhang ZS, Tang C, Bair C. L, Landar A, Landar A, Zimmer DB and Weber DJ. (1999) Letter to the Editor: H-1, C-13 and N-15 NMR sequence-specific resonance assignments for rat apo-S100A1(alpha alpha).J Biomol NMR, 14,91-92 

1. ANTIVIRAL INHIBITION OF CAPSID PROTEINS, 2003,  Patent No. US 7361459 B2

2.  ANTIVIRAL INHIBITION OF CAPSID PROTEINS, 2003,  Patent No. WO2003089615

3.  Gadolinium complex as a probe for paramagnetic relaxation enhancement of solution and its synthesis method, 2015, Patent No. CN103524368B

4.  A method for the preparation of protein labeled tri-metachlorine fluorescence probe, 2014, Patent No. 2014100770618

5.  A water soluble near-infrared fluorescence probe for protein labeling and its synthesis method, 2014, Patent No.  201410075670X

6.  A Method for Fast Fluorescent Labeling of Proteins, 2015, Patent No. 20150080389X

7.  Preparation of crosslinked NAMPT and screening of NAMPT inhibitors, 2014, Patent No. 201410061169.8

We are interested how proteins and nucleic acids take up various shapes while performing their functions.



To this end, we employ both experimental and computational appraoches, and we use a combination of biochemical and biophysical techniques, including but not limted to nuclear magnetic resonance (NMR), small-angle X-ray scattering (SAXS), chemical cross-linking coupled with mass spectrometry, single-molecule fluorescence resonance energy transfer (smFRET). 



Currently we are investigating how K63-linked polyubiquitin mediates cell siganaling and how methylation affects RNA dynamics.


（1）Visualizing protein dynamics with paramagnetic NMR, The Fifth National Symposium on Interdisciplinary Protein Research, 2015-10-17
（2）Visualizing macromolecular dynamics using solution based techniques, Hybrid Methods for Integrative Structural Biology Workshop (ALS/LCLS/SSRL), 2015-10-05
（3）Visualizing the quaternary dynamics of Lys63-linked diubiquitin, 2015 Metal Protein Science Conference 2015-09-12
（4）Depicting the quaternary structure of K63-linked diubiquitin using PRE NMR, The 19th International Society of Magnetic Resonance (ISMAR 2015), 2015-08-15
（5）What drives the morphological switch of HIV-1 capsid?   International Symposium on Magnetic Resonance, 2015-08-15
（6）Fleeting Protein transient complex and pohsphoric acid signal transduction, 2014 National Conference on Biochemistry and Molecular Biology, 2014-08-21
（7）Some Mathematical Problems in Visualizing Protein Dynamics, 20th International Conference on Difference Equations and Applications (ICDEA2OL4), 2014-07-21
（8）The Conformational Space of Lys63-linked Diubiquitin Enables Specific Target Recognition, Structural Biology Frontier (host by 《Cell》 magazine), 2014-05-15
（9）Visualizing fleeting protein-protein interactions using PRE NMR, Asia-Pacific Nuclear Magnetic Resonance Conference, 2013-10-24
（10）Fleeting Protein Complexes Visualized by PRE NMR, The 4th China Symposium on Structural Biology, 2013-04-01
（11）Direct Visualization of Protein Dynamics Using PRE NMR, The 17th Chinese Society of Physics Spectroscopy Conference and Cross - Strait Magnetic Resonance Conference, 2012-10-30
（12）Direct Visualization of Protein Dynamics Using PRE NMR, Weak Protein-Ligand Interactions: New Horizons in Biophysics and Cell Biology,  2012-10-14
（13）Direct Visualization of Protein Dynamics Using PRE NMR, The 41st Korean Magnetic Resonance Society Symposium, 2012-06-28
（14）Directive Visualization of Protein Dynamics with PRE NMR, The 17th IUPAB International Biophysics Congress, 2011-10-30
（15）Rigid Attachment of Paramagnetic Probes for Visualizing Protein Structure and Dyanmics, The 14th Beijing Analytical Testing Symposium and Exhibition, 2011-10-13
（16）PRE Dynamics Ensemble Analysis, Biological macromolecule NMR summer workshop,  2011-06-20
（17）Paramagnetic Relaxation Enhancement for Studying Protein Solution Dynamics, Seminar on Nuclear Magnetic Resonance, 2011-02-16
（18）Recent Development of Paramagnetic Relaxation Enhancement in Studying Protein Solution Dynamics, The 16th Chinese Society of Physics Spectroscopy Conference, 2010-11-24
（19）DiSPRE, Tagging Different Paramagnetic Probes in Characterizing Protein Dynamics, Seminar on Paramagnetic Labeling (FEBS), 2010-05-16
（20）Characterizing Dynamic Protein-Protein Interactions Using Differentially Scaled Paramagnetic Relaxation Enhancement, Gordon Research Conference on Biomolecular Interaction and Methods, 2010-01-17
（21）Visualizing the Maturation of HIV-1 Protease by Paramagnetic NMR, Great Plains Regional Annual Symposium on Protein NMR, 2008-10-03
（22）Visualizing Transient Low-Population Intermediates by Paramagnetic Relaxation Enhancemen, Keystone Symposium, Frontiers in Structural Biology,  2008-01-06

HHMI International Early Career Scientist

National Outstanding Young Scientist

National Key Basic Research Program Chief Scientist