蛋白质纯化，制剂工程，疫苗工程

   

081703-生物化工

蛋白质纯化，制剂工程，mRNA，药物递送，疫苗工程，纳米材料

   

（ 1 ） 一种在体外转录过程中减少或抑制双链核糖核酸形成的mRNA高产制备方法, 发明专利, 2023, 第 4 作者, 专利号: CN116536307A

（ 2 ） 可减少或抑制dsRNA形成的用于体外转录合成mRNA的试剂盒及其使用方法, 发明专利, 2023, 第 3 作者, 专利号: CN116536306A

（ 3 ） 一种mRNA的加帽方法, 发明专利, 2023, 第 4 作者, 专利号: CN115948382A

（ 4 ） 一种核糖核酸纯化伴侣及其应用, 发明专利, 2022, 第 3 作者, 专利号: CN114990109A

（ 5 ） 一种mRNA的分离纯化方法, 发明专利, 2022, 第 2 作者, 专利号: CN114907430A

（ 6 ） 一种肿瘤光热制剂及其应用, 发明专利, 2022, 第 4 作者, 专利号: CN113925970A

（ 7 ） 一种肿瘤光热制剂的应用, 2023, 第 4 作者, 专利号: CN113925970B

（ 8 ） 一种多抗原仿生流感疫苗及其制备方法和应用, 专利授权, 2019, 第 4 作者, 专利号: CN109821014A

（ 9 ） 一种基于铁蛋白的多抗原通用流感疫苗及其制备方法和应用, 专利授权, 2019, 第 4 作者, 专利号: CN109966483A

（ 10 ） 一种以HBc-VLP为载体的装载系统的制备方法, 专利授权, 2019, 第 3 作者, 专利号: CN109675055A

（ 11 ） 一种纯化HBc-VLPs或HBc-VLPs衍生物的方法, 发明专利, 2019, 第 3 作者, 专利号: CN109134620A

（ 12 ） 一种HBcAg-VLP或HBcAg-VLP衍生物的纯化方法, 发明专利, 2019, 第 5 作者, 专利号: CN109134621A

（ 13 ） 一种细菌多糖结合疫苗及其制备方法与应用, 2022, 第 5 作者, 专利号: CN108743936B

（ 14 ） 一种细菌多糖结合疫苗及其制备方法与应用, 发明专利, 2018, 第 5 作者, 专利号: CN108743936A

   

[1] Xue Feng, Zhengjun Li, Zhiguo Su, Shiyi Che, Baiqian Dai, Yuan Cheng, Songping Zhang. Rapid and high recovery isolation of mRNA from in vitro transcription system by ammonium sulphate precipitation at room temperature. SEPARATION AND PURIFICATION TECHNOLOGY. 2024, 第 2 作者336: http://dx.doi.org/10.1016/j.seppur.2024.126331.
[2] Sheng Yanan, Li Zhengjun, Lin Xuan, Wang Liuyang, Zhu Hongyu, Su Zhiguo, Zhang Songping. In situ bio-mineralized Mn nanoadjuvant enhances anti-influenza immunity of recombinant virus-like particle vaccines. Journal of Controlled Release[J]. 2024, 第 2 作者
[3] Sheng, Yanan, Li, Zhengjun, Lin, Xuan, Ma, Yanyan, Ren, Ying, Su, Zhiguo, Ma, Guanghui, Zhang, Songping. The position of Spy Tag/Catcher system in hepatitis B core protein particles affects the immunogenicity and stability of the synthetic vaccine. VACCINE[J]. 2023, 第 2 作者41(33): 4867-4878, http://dx.doi.org/10.1016/j.vaccine.2023.06.070.
[4] Wang, Liuyang, Lin, Xuan, Sheng, Yanan, Zhu, Hongyu, Li, Zhengjun, Su, Zhiguo, Yu, Rong, Zhang, Songping. Synthesis of a crystalline zeolitic imidazole framework-8 nano-coating on single environment-sensitive viral particles for enhanced immune responses. NANOSCALE ADVANCES[J]. 2023, 第 5 作者5(5): 1433-1449, http://dx.doi.org/10.1039/d2na00767c.
[5] Zhengjun Li, Yanyan Ma, Ren, Ying, Xuan Lin, Su, Zhiguo, Songping Zhang. Thermal-triggered loading and GSH-responsive releasing property of HBc particles for drug delivery. Journal of Controlled Release[J]. 2023, 第 1 作者784-796, 
[6] 郝建敏, 刘幽燕, 苏志国, 张松平, 李正军. 高效液相尺寸排阻色谱-多角度激光散射定量检测灭活禽流感病毒抗原中完整病毒颗粒. 生物工程学报[J]. 2023, 第 5 作者  通讯作者  39(10): 4295-4307, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=7579252&detailType=1.
[7] Lin, Xuan, Sheng, Yanan, Zhang, Xuan, Li, Zhengjun, Yang, Yanli, Wu, Jie, Su, Zhiguo, Ma, Guanghui, Zhang, Songping. Oil-in-ionic liquid nanoemulsion-based intranasal delivery system for influenza split-virus vaccine. JOURNAL OF CONTROLLED RELEASE[J]. 2022, 第 4 作者346: 380-391, http://dx.doi.org/10.1016/j.jconrel.2022.04.036.
[8] Xuan Lin, Yanli Yang, Shuai Li, Zhengjun Li, Yanan Sheng, Zhiguo Su, Songping Zhang. Oil-in-ionic liquid nanoemulsion-based adjuvant simultaneously enhances the stability and immune responses of inactivated foot-and-mouth disease virus. INTERNATIONAL JOURNAL OF PHARMACEUTICS. 2022, 第 4 作者625: http://dx.doi.org/10.1016/j.ijpharm.2022.122083.
[9] Kang, Yong, Li, Zhengjun, Lu, Fengying, Su, Zhiguo, Ji, Xiaoyuan, Zhang, Songping. Synthesis of red/black phosphorus-based composite nanosheets with a Z-scheme heterostructure for high-performance cancer phototherapy. NANOSCALE[J]. 2022, 第 2 作者14(3): 766-779, 
[10] Sheng, Yanan, Wei, Jiangxue, Li, Zhengjun, Su, Zhiguo, Ma, Guanghui, Zhang, Songping. Apoferritin nanoparticle based dual-antigen influenza conjugate vaccine with potential cross-protective efficacy against heterosubtypic influenza virus. PARTICUOLOGY[J]. 2022, 第 3 作者64(5): 56-64, http://dx.doi.org/10.1016/j.partic.2021.04.001.
[11] Lu, Fengying, Li, Zhengjun, Sheng, Yanan, Ma, Yanyan, Yang, Yanli, Ren, Ying, Su, Zhiguo, Yu, Rong, Zhang, Songping. Thermal-triggered packing of lipophilic NIR dye IR780 in hepatitis B core at critical ionic strength and cargo-host ratio for improved stability and enhanced cancer phototherapy. BIOMATERIALS[J]. 2021, 第 2 作者276: http://dx.doi.org/10.1016/j.biomaterials.2021.121035.
[12] Song, Yanmin, Yang, Yanli, Lin, Xuan, Zhao, Qizu, Li, Zhengjun, Ma, Guanghui, Su, Zhiguo, Zhang, Songping. On-line separation and quantification of virus antigens of different serotypes in multivalent vaccines by capillary zone electrophoresis: A case study for quality control of foot-and-mouth disease virus vaccines. JOURNAL OF CHROMATOGRAPHY A[J]. 2021, 第 5 作者1637: http://dx.doi.org/10.1016/j.chroma.2020.461834.
[13] 马艳艳, 李正军, 张松平, 陈卫, 任瑛. HBc-VLP的分子动力学模拟和结合自由能计算. 过程工程学报[J]. 2021, 第 2 作者21(2): 219-229, http://lib.cqvip.com/Qikan/Article/Detail?id=7104287977.
[14] Kang, Yong, Li, Zhengjun, Lu, Fengying, Su, Zhiguo, Ji, Xiaoyuan, Zhang, Songping. Synthesis of red/black phosphorus-based composite nanosheets with a Z-scheme heterostructure for high-performance cancer phototherapy. NANOSCALE[J]. 2021, 第 2 作者http://dx.doi.org/10.1039/d1nr07553e.
[15] Kang, Yong, Li, Zhengjun, Yang, Yanli, Su, Zhiguo, Ji, Xiaoyuan, Zhang, Songping. Antimonene Nanosheets-Based Z-Scheme Heterostructure with Enhanced Reactive Oxygen Species Generation and Photothermal Conversion Efficiency for Photonic Therapy of Cancer. ADVANCED HEALTHCARE MATERIALS[J]. 2021, 第 2 作者10(3): http://dx.doi.org/10.1002/adhm.202001835.
[16] Yang, Yanli, Song, Yanmin, Lin, Xuan, Li, Shuai, Li, Zhengjun, Zhao, Qizu, Ma, Guanghui, Zhang, Songping, Su, Zhiguo. Mechanism of bio-macromolecule denaturation on solid-liquid surface of ion-exchange chromatographic media - A case study for inactivated foot-and-mouth disease virus. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES[J]. 2020, 第 5 作者1142: http://dx.doi.org/10.1016/j.jchromb.2020.122051.
[17] Kang, Yong, Ji, Xiaoyuan, Li, Zhengjun, Su, Zhiguo, Zhang, Songping. Boron-based nanosheets for combined cancer photothermal and photodynamic therapy. JOURNAL OF MATERIALS CHEMISTRY B[J]. 2020, 第 3 作者8(21): 4609-4619, https://www.webofscience.com/wos/woscc/full-record/WOS:000538170900005.
[18] Li, Shuai, Yang, Yanli, Lin, Xuan, Li, Zhengjun, Ma, Guanghui, Su, Zhiguo, Zhang, Songping. Biocompatible cationic solid lipid nanoparticles as adjuvants effectively improve humoral and T cell immune response of foot and mouth disease vaccines. VACCINE[J]. 2020, 第 4 作者38(11): 2478-2486, http://dx.doi.org/10.1016/j.vaccine.2020.02.004.
[19] Li, Shuai, Yang, Yanli, Lin, Xuan, Li, Zhengjun, Ma, Guanghui, Su, Zhiguo, Zhang, Songping. Biocompatible cationic solid lipid nanoparticles as adjuvants effectively improve humoral and T cell immune response of foot and mouth disease vaccines. VACCINE[J]. 2020, 第 4 作者38(11): 2478-2486, http://dx.doi.org/10.1016/j.vaccine.2020.02.004.
[20] Wei, Jiangxue, Li, Zhengjun, Yang, Yanli, Ma, Xiaowei, An, Wenqi, Ma, Guanghui, Su, Zhiguo, Zhang, Songping. A biomimetic VLP influenza vaccine with interior NP/exterior M2e antigens constructed through a temperature shift-based encapsulation strategy. VACCINE[J]. 2020, 第 2 作者38(38): 5987-5996, http://dx.doi.org/10.1016/j.vaccine.2020.07.015.
[21] Wei, Jiangxue, Li, Zhengjun, Yang, Yanli, Ma, Guanghui, Su, Zhiguo, Zhang, Songping. An Apoferritin-Hemagglutinin Conjugate Vaccine with Encapsulated Nucleoprotein Antigen Peptide from Influenza Virus Confers Enhanced Cross Protection. BIOCONJUGATE CHEMISTRY[J]. 2020, 第 2 作者31(8): 1948-1959, http://dx.doi.org/10.1021/acs.bioconjchem.0c00308.
[22] Li, Shuai, Yang, Yanli, Lin, Xuan, Li, Zhengjun, Ma, Guanghui, Su, Zhiguo, Zhang, Songping. A Novel Particulate Delivery System Based on Antigen-Zn2+ Coordination Interactions Enhances Stability and Cellular Immune Response of Inactivated Foot and Mouth Disease Virus Shuai. MOLECULAR PHARMACEUTICS[J]. 2020, 第 4 作者17(8): 2952-2963, https://www.webofscience.com/wos/woscc/full-record/WOS:000558726700017.
[23] Lu, Fengying, Li, Zhengjun, Kang, Yong, Su, Zhiguo, Yu, Rong, Zhang, Songping. Black phosphorus quantum dots encapsulated in anionic waterborne polyurethane nanoparticles for enhancing stability and reactive oxygen species generation for cancer PDT/PTT therapy. JOURNAL OF MATERIALS CHEMISTRY B[J]. 2020, 第 2 作者8(46): 10650-10661, https://www.webofscience.com/wos/woscc/full-record/WOS:000608190300017.
[24] Xu, Lingling, Li, Zhengjun, Su, Zhiguo, Yang, Yanli, Ma, Guanghui, Yu, Rong, Zhang, Songping. Development of meningococcal polysaccharide conjugate vaccine that can elicit long-lasting and strong cellular immune response with hepatitis B core antigen virus-like particles as a novel carrier protein. VACCINE[J]. 2019, 第 2 作者37(7): 956-964, http://ir.ipe.ac.cn/handle/122111/27945.
[25] Li, Zhengjun, Wei, Jiangxue, Yang, Yanli, Liu, Lili, Ma, Guanghui, Zhang, Songping, Su, Zhiguo. A two-step heat treatment of cell disruption supernatant enables efficient removal of host cell proteins before chromatographic purification of HBc particles. JOURNAL OF CHROMATOGRAPHY A[J]. 2018, 第 1 作者1581: 71-79, http://ir.ipe.ac.cn/handle/122111/27589.
[26] 胥玲玲, 李正军, 苏志国, 杨延丽, 余蓉, 郑永祥, 张松平. 以乙肝核心抗原病毒样颗粒为新型载体蛋白制备流脑多糖结合疫苗. 华西药学杂志[J]. 2018, 第 2 作者33(6): 463-467, https://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFQ&dbname=CJFDLAST2019&filename=HXYO201806001&v=MjE2NDdlWitSdkZpamtWTHpCTFRYU1liRzRIOW5NcVk5RlpZUjhlWDFMdXhZUzdEaDFUM3FUcldNMUZyQ1VSN3E=.
[27] Li, Zhengjun, Wei, Jiangxue, Yang, Yanli, Ma, Xiaowei, Hou, Baidong, An, Wenqi, Hua, Zhaolin, Zhang, Jingjing, Li, Yao, Ma, Guanghui, Zhang, Songping, Su, Zhiguo. Strong hydrophobicity enables efficient purification of HBc VLPs displaying various antigen epitopes through hydrophobic interaction chromatography. BIOCHEMICAL ENGINEERING JOURNAL[J]. 2018, 第 1 作者140: 157-167, http://dx.doi.org/10.1016/j.bej.2018.09.020.
[28] Chen, Zenghui, Zhang, Songping, Li, Zhengjun, Ma, Guanghui, Su, Zhiguo. Construction of a stable w/o nano-emulsion as a potential adjuvant for foot and mouth disease virus vaccine. ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY[J]. 2017, 第 3 作者45(5): 897-906, http://ir.ipe.ac.cn/handle/122111/22623.
[29] Yang, Yanli, Zhao, Qizu, Li, Zhengjun, Sun, Lijing, Ma, Guanghui, Zhang, Songping, Su, Zhiguo. Stabilization study of inactivated foot and mouth disease virus vaccine by size-exclusion HPLC and differential scanning calorimetry. VACCINE[J]. 2017, 第 3 作者35(18): 2413-2419, http://dx.doi.org/10.1016/j.vaccine.2017.03.037.
[30] Li, Zenglan, Zhang, Yan, Wang, Qi, Li, Zhengjun, Liu, Yongdong, Zhang, Songping, Zhang, Guifeng, Ma, Guanghui, Luo, Jian, Su, Zhiguo. Purification design and practice for pertactin, the third component of acellular pertussis vaccine, from Bordetella pertussis. VACCINE[J]. 2016, 第 4 作者34(34): 4032-4039, http://dx.doi.org/10.1016/j.vaccine.2016.06.029.
[31] 李正军, 魏江雪, 张松平, 苏志国. 类病毒颗粒HBcAg的热稳定性及小分子的进入. 2015年中国化工学会年会论文集. 2015, 第 1 作者http://ir.ipe.ac.cn/handle/122111/20410.
[32] Yang, Yanli, Li, Hao, Li, Zhengjun, Zhang, Yan, Zhang, Songping, Chen, Yi, Yu, Mengran, Ma, Guanghui, Su, Zhiguo. Size-exclusion HPLC provides a simple, rapid, and versatile alternative method for quality control of vaccines by characterizing the assembly of antigens. VACCINE[J]. 2015, 第 3 作者33(9): 1143-1150, http://dx.doi.org/10.1016/j.vaccine.2015.01.031.