基本信息
张燕飞 男 博导 中国科学院天津工业生物技术研究所
电子邮件： zhangyf@tib.cas.cn
通信地址： 天津空港经济区西七道32号
邮政编码： 300308
电子邮件： zhangyf@tib.cas.cn
通信地址： 天津空港经济区西七道32号
邮政编码： 300308
招生信息
招生专业
071010-生物化学与分子生物学
071005-微生物学
071005-微生物学
招生方向
合成生物学，代谢工程，蛋白质工程，光遗传学
一碳代谢，合成生物能量学
生物材料
一碳代谢，合成生物能量学
生物材料
教育背景
2010-09--2015-05 University of Alberta 博士
工作经历
工作简历
2015-09~2020-08,Princeton University, 博士后
社会兼职
2023-04-01-今, 天津市微生物学会理事, 理事
2021-05-05-今, 天津市石油和化学工业协会常务理事, 常务理事
2021-05-05-今, 天津市石油和化学工业协会常务理事, 常务理事
专利与奖励
专利成果
[1] 张燕飞. 一种毕赤酵母光控转录调节系统及其应用. 2024105992238, 2024-05-15.
[2] 张燕飞. 光控核酸内切酶 OptoCas 及其应用. 202410482667.3, 2024-04-18.
[3] 张燕飞. 一种甲酸利用的解脂耶氏酵母及其制备方法. 2024104200320, 2024-04-09.
[4] 张燕飞. 一种菌酶联用固化沙漠风积砂的方法. ZL 2023110490862.2, 2023-12-02.
[5] 张燕飞. 新型光控阻遏蛋白 OptoLacI 及其应用方法. 202310707956.4, 2023-06-15.
[6] 张燕飞. 一种基于S-腺苷甲硫氨酸合成酶突变体的L-甲硫氨酸生物传感器及其应用. 2023104975052, 2023-05-05.
[7] 张燕飞. 一种去除水体中重金属的金属吸附装置及处理系统. 2023209510080, 2023-04-25.
[8] 尤晓颜, 陈玥, 赵国屏, 张燕飞, 布青云, 丁玥, 朱月, 王慧. 一种具有肠道靶向释放功能的油凝胶微球及其制备方法. CN: CN115252575A, 2022-11-01.
[9] 张燕飞. 一种基于split GFP定量评估微生物细胞表面展示效率的方法. 2022108807333, 2022-07-26.
[10] 张燕飞, 黄建峰, 蔡世杰, 尤晓颜, 赵国屏. 一种用于响应胞内L-甲硫氨酸浓度的生物传感器. CN: CN116445427A, 2023-07-18.
[11] 张燕飞, 谭玲, 尤晓颜, 赵国屏. 一种检测微生物吸附金属离子能力的方法. CN: CN113758903A, 2021-12-07.
[2] 张燕飞. 光控核酸内切酶 OptoCas 及其应用. 202410482667.3, 2024-04-18.
[3] 张燕飞. 一种甲酸利用的解脂耶氏酵母及其制备方法. 2024104200320, 2024-04-09.
[4] 张燕飞. 一种菌酶联用固化沙漠风积砂的方法. ZL 2023110490862.2, 2023-12-02.
[5] 张燕飞. 新型光控阻遏蛋白 OptoLacI 及其应用方法. 202310707956.4, 2023-06-15.
[6] 张燕飞. 一种基于S-腺苷甲硫氨酸合成酶突变体的L-甲硫氨酸生物传感器及其应用. 2023104975052, 2023-05-05.
[7] 张燕飞. 一种去除水体中重金属的金属吸附装置及处理系统. 2023209510080, 2023-04-25.
[8] 尤晓颜, 陈玥, 赵国屏, 张燕飞, 布青云, 丁玥, 朱月, 王慧. 一种具有肠道靶向释放功能的油凝胶微球及其制备方法. CN: CN115252575A, 2022-11-01.
[9] 张燕飞. 一种基于split GFP定量评估微生物细胞表面展示效率的方法. 2022108807333, 2022-07-26.
[10] 张燕飞, 黄建峰, 蔡世杰, 尤晓颜, 赵国屏. 一种用于响应胞内L-甲硫氨酸浓度的生物传感器. CN: CN116445427A, 2023-07-18.
[11] 张燕飞, 谭玲, 尤晓颜, 赵国屏. 一种检测微生物吸附金属离子能力的方法. CN: CN113758903A, 2021-12-07.
出版信息
发表论文
[1] Li Zhang, Tan, Lin, Zhang, Yanfei. Quantitative measurement of cell-surface displayed proteins based on split-GFP assembly. Microbial Cell Factories[J]. 2024, 23(108): https://doi.org/10.1186/s12934-024-02386-1.
[2] 张燕飞. Engineering of a substrate affinity reduced S-adenosyl-methionine synthase as a novel biosensor for growth-coupling selection of L-methionine overproducers. Applied Biochemistry and Biotechnology[J]. 2024, 196: https://doi.org/10.1007/s12010-023-04807-0.
[3] Ling Tan, Meizi Liu, Lixian Wang, Guoping Zhao, Yanfei Zhang. Flow cytometry-based high-throughput screening of synthetic peptides for palladium adsorption. JOURNAL OF HAZARDOUS MATERIALS. 2024, 461: http://dx.doi.org/10.1016/j.jhazmat.2023.132656.
[4] Xue Yang, Yanfei Zhang, Guoping Zhao. Artificial carbon assimilation: From unnatural reactions and pathways to synthetic autotrophic systems. BIOTECHNOLOGY ADVANCES. 2024, 70: http://dx.doi.org/10.1016/j.biotechadv.2023.108294.
[5] Xue Yang, Zhitao Mao, Jianfeng Huang, Ruoyu Wang, Huaming Dong, Yanfei Zhang, Hongwu Ma. Improving pathway prediction accuracy of constraints-based metabolic network models by treating enzymes as microcompartments. SYNTHETIC AND SYSTEMS BIOTECHNOLOGY[J]. 2023, 8(4): 597-605, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10514394/.
[6] Tan, Ling, Liu, Xindi, Zhang, Yanfei. Glutaraldehyde fixation promotes palladium and gold nanoparticles formation in yeast and enhances their catalytic activity in 4-nitrophenol reduction. JOURNAL OF HAZARDOUS MATERIALS[J]. 2023, 446: http://dx.doi.org/10.1016/j.jhazmat.2022.130696.
[7] Li, Zuhui, Ge, Ran, Tan, Ling, Zhang, Wuyuan, Zhang, Yanfei. Photodecarboxylase Displayed on Microbial Cell-Surface for Enhanced Biocatalytic Performance. CHEMCATCHEM[J]. 2023, 15(14): http://dx.doi.org/10.1002/cctc.202300494.
[8] Huaming Dong, Xue Yang, Jingjing Shi, Chunqiao Xiao, 张燕飞. Exploring the Feasibility of Cell-Free Synthesis as a Platform for Polyhydroxyalkanoate (PHA) Production: Opportunities and Challenges. Polymers[J]. 2023, https://doi.org/10.3390/polym15102333.
[9] 褚鑫, 王力为, 许虹, 张燕飞. 工业生物技术的前沿科技. 生物工程学报[J]. 2022, 38(11): 4019-4026, http://lib.cqvip.com/Qikan/Article/Detail?id=7108545579.
[10] Zhang, Yanfei, Cortez, Jeremy D, Hammer, Sarah K, CarrascoLopez, Cesar, Echauri, Sergio A Garcia, Wiggins, Jessica B, Wang, Wei, Avalos, Jose L. Biosensor for branched-chain amino acid metabolism in yeast and applications in isobutanol and isopentanol production. NATURE COMMUNICATIONS[J]. 2022, 13(1): http://dx.doi.org/10.1038/s41467-021-27852-x.
[11] Scott A Wegner, Jhong-Min Chen, Samantha S Ip, Yanfei Zhang, Deepak Dugar, José L. Avalos. Engineering acetyl-CoA supply and ERG9 repression to enhance mevalonate production in Saccharomyces cerevisiae. Journal of Industrial Microbiology & Biotechnology[J]. 2021,
[12] Hammer, Sarah K, Zhang, Yanfei, Avalos, Jose L. Mitochondrial Compartmentalization Confers Specificity to the 2-Ketoacid Recursive Pathway: Increasing Isopentanol Production in Saccharomyces cerevisiae. ACS SYNTHETIC BIOLOGY[J]. 2020, 9(3): 546-555, https://www.webofscience.com/wos/woscc/full-record/WOS:000526388400011.
[13] Stephan Lane, Zhang Yanfei, Eun Ju Yun, Leah Ziolkowski, Guochang Zhang, Yong-Su Jin, José L. Avalos. Xylose assimilation enhances production of isobutanol in engineered Saccharomyces cerevisiae.. Biotechnology and Bioengineering[J]. 2020, 117(2): 372-381, https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/bit.27202.
[14] Alvarez, Maria S, Zhang, Yanfei. Sketching neoteric solvents for boosting drugs bioavailability. JOURNAL OF CONTROLLED RELEASE. 2019, 311: 225-232, http://dx.doi.org/10.1016/j.jconrel.2019.09.008.
[15] Zhang, Yanfei, Lane, Stephan, Chen, JhongMin, Hammer, Sarah K, Luttinger, Jake, Yang, Lifeng, Jin, YongSu, Avalos, Jose L. Xylose utilization stimulates mitochondrial production of isobutanol and 2-methyl-1-butanol in Saccharomyces cerevisiae. BIOTECHNOLOGY FOR BIOFUELS[J]. 2019, 12(1): http://dx.doi.org/10.1186/s13068-019-1560-2.
[16] Zhao, Evan M, Zhang, Yanfei, Mehl, Justin, Park, Helen, Lalwani, Makoto A, Toettcher, Jared E, Avalos, Jose L. Optogenetic regulation of engineered cellular metabolism for microbial chemical production. NATURE[J]. 2018, 555(7698): 683-+, https://www.webofscience.com/wos/woscc/full-record/WOS:000428617600054.
[17] Zhang, Yanfei, Avalos, Jose L. Traditional and novel tools to probe the mitochondrial metabolism in health and disease. WILEY INTERDISCIPLINARY REVIEWS-SYSTEMS BIOLOGY AND MEDICINE. 2017, 9(2): https://www.webofscience.com/wos/woscc/full-record/WOS:000394898500006.
[18] Zhang Yanfei. The quinone-binding site of Acidithiobacillus ferrooxidans sulfide:quinone oxidoreductase controls both sulfide oxidation and quinone reduction.. Biochemistry and Cell Biology. 2015,
[19] Zhang, Yanfei, Weiner, Joel H. A simple semi-quantitative in vivo method using H2S detection to monitor sulfide metabolizing enzymes. BIOTECHNIQUES[J]. 2014, 57(4): 208-210, https://www.webofscience.com/wos/woscc/full-record/WOS:000343303600007.
[20] Zhang, Yanfei, Weiner, Joel H. Characterization of the kinetics and electron paramagnetic resonance spectroscopic properties of Acidithiobacillus ferrooxidans sulfide:quinone oxidoreductase (SQR). ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS[J]. 2014, 564: 110-119, https://www.webofscience.com/wos/woscc/full-record/WOS:000346222600013.
[21] Zhang, Yanfei, Yang, Yu, Liu, Jianshe, Qiu, Guanzhou. Isolation and characterization of Acidithiobacillus ferrooxidans strain QXS-1 capable of unusual ferrous iron and sulfur utilization. HYDROMETALLURGY[J]. 2013, 136: 51-57, http://dx.doi.org/10.1016/j.hydromet.2013.03.005.
[22] Zhang, Yanfei, Peng, Anan, Yang, Yu, Liu, Jianshe, Qiu, Guanzhou. Isolation, characterization of Acidiphilium sp DX1-1 and ore bioleaching by this acidophilic mixotrophic organism. TRANSACTIONS OF NONFERROUS METALS SOCIETY OF CHINA[J]. 2013, 23(6): 1774-1782, https://www.webofscience.com/wos/woscc/full-record/WOS:000324003500035.
[23] Cherney, Maia M, Zhang, Yanfei, James, Michael N G, Weiner, Joel H. Structure-activity characterization of sulfide:quinone oxidoreductase variants. JOURNAL OF STRUCTURAL BIOLOGY[J]. 2012, 178(3): 319-328, https://www.webofscience.com/wos/woscc/full-record/WOS:000305052200011.
[24] Cherney, Maia M, Zhang, Yanfei, Solomonson, Matthew, Weiner, Joel H, James, Michael N G. Crystal Structure of Sulfide:Quinone Oxidoreductase from Acidithiobacillus ferrooxidans: Insights into Sulfidotrophic Respiration and Detoxification. JOURNAL OF MOLECULAR BIOLOGY[J]. 2010, 398(2): 292-305, http://dx.doi.org/10.1016/j.jmb.2010.03.018.
[25] Li Zhang, Ling Tan, Meizi Liu, Yunhong Chen, Yu Yang, Yanfei Zhang, Guoping Zhao. Quantitative measurement of cell-surface displayed proteins based on split-GFP assembly. MICROBIAL CELL FACTORIES. 23(1): http://dx.doi.org/10.1186/s12934-024-02386-1.
[2] 张燕飞. Engineering of a substrate affinity reduced S-adenosyl-methionine synthase as a novel biosensor for growth-coupling selection of L-methionine overproducers. Applied Biochemistry and Biotechnology[J]. 2024, 196: https://doi.org/10.1007/s12010-023-04807-0.
[3] Ling Tan, Meizi Liu, Lixian Wang, Guoping Zhao, Yanfei Zhang. Flow cytometry-based high-throughput screening of synthetic peptides for palladium adsorption. JOURNAL OF HAZARDOUS MATERIALS. 2024, 461: http://dx.doi.org/10.1016/j.jhazmat.2023.132656.
[4] Xue Yang, Yanfei Zhang, Guoping Zhao. Artificial carbon assimilation: From unnatural reactions and pathways to synthetic autotrophic systems. BIOTECHNOLOGY ADVANCES. 2024, 70: http://dx.doi.org/10.1016/j.biotechadv.2023.108294.
[5] Xue Yang, Zhitao Mao, Jianfeng Huang, Ruoyu Wang, Huaming Dong, Yanfei Zhang, Hongwu Ma. Improving pathway prediction accuracy of constraints-based metabolic network models by treating enzymes as microcompartments. SYNTHETIC AND SYSTEMS BIOTECHNOLOGY[J]. 2023, 8(4): 597-605, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10514394/.
[6] Tan, Ling, Liu, Xindi, Zhang, Yanfei. Glutaraldehyde fixation promotes palladium and gold nanoparticles formation in yeast and enhances their catalytic activity in 4-nitrophenol reduction. JOURNAL OF HAZARDOUS MATERIALS[J]. 2023, 446: http://dx.doi.org/10.1016/j.jhazmat.2022.130696.
[7] Li, Zuhui, Ge, Ran, Tan, Ling, Zhang, Wuyuan, Zhang, Yanfei. Photodecarboxylase Displayed on Microbial Cell-Surface for Enhanced Biocatalytic Performance. CHEMCATCHEM[J]. 2023, 15(14): http://dx.doi.org/10.1002/cctc.202300494.
[8] Huaming Dong, Xue Yang, Jingjing Shi, Chunqiao Xiao, 张燕飞. Exploring the Feasibility of Cell-Free Synthesis as a Platform for Polyhydroxyalkanoate (PHA) Production: Opportunities and Challenges. Polymers[J]. 2023, https://doi.org/10.3390/polym15102333.
[9] 褚鑫, 王力为, 许虹, 张燕飞. 工业生物技术的前沿科技. 生物工程学报[J]. 2022, 38(11): 4019-4026, http://lib.cqvip.com/Qikan/Article/Detail?id=7108545579.
[10] Zhang, Yanfei, Cortez, Jeremy D, Hammer, Sarah K, CarrascoLopez, Cesar, Echauri, Sergio A Garcia, Wiggins, Jessica B, Wang, Wei, Avalos, Jose L. Biosensor for branched-chain amino acid metabolism in yeast and applications in isobutanol and isopentanol production. NATURE COMMUNICATIONS[J]. 2022, 13(1): http://dx.doi.org/10.1038/s41467-021-27852-x.
[11] Scott A Wegner, Jhong-Min Chen, Samantha S Ip, Yanfei Zhang, Deepak Dugar, José L. Avalos. Engineering acetyl-CoA supply and ERG9 repression to enhance mevalonate production in Saccharomyces cerevisiae. Journal of Industrial Microbiology & Biotechnology[J]. 2021,
[12] Hammer, Sarah K, Zhang, Yanfei, Avalos, Jose L. Mitochondrial Compartmentalization Confers Specificity to the 2-Ketoacid Recursive Pathway: Increasing Isopentanol Production in Saccharomyces cerevisiae. ACS SYNTHETIC BIOLOGY[J]. 2020, 9(3): 546-555, https://www.webofscience.com/wos/woscc/full-record/WOS:000526388400011.
[13] Stephan Lane, Zhang Yanfei, Eun Ju Yun, Leah Ziolkowski, Guochang Zhang, Yong-Su Jin, José L. Avalos. Xylose assimilation enhances production of isobutanol in engineered Saccharomyces cerevisiae.. Biotechnology and Bioengineering[J]. 2020, 117(2): 372-381, https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/bit.27202.
[14] Alvarez, Maria S, Zhang, Yanfei. Sketching neoteric solvents for boosting drugs bioavailability. JOURNAL OF CONTROLLED RELEASE. 2019, 311: 225-232, http://dx.doi.org/10.1016/j.jconrel.2019.09.008.
[15] Zhang, Yanfei, Lane, Stephan, Chen, JhongMin, Hammer, Sarah K, Luttinger, Jake, Yang, Lifeng, Jin, YongSu, Avalos, Jose L. Xylose utilization stimulates mitochondrial production of isobutanol and 2-methyl-1-butanol in Saccharomyces cerevisiae. BIOTECHNOLOGY FOR BIOFUELS[J]. 2019, 12(1): http://dx.doi.org/10.1186/s13068-019-1560-2.
[16] Zhao, Evan M, Zhang, Yanfei, Mehl, Justin, Park, Helen, Lalwani, Makoto A, Toettcher, Jared E, Avalos, Jose L. Optogenetic regulation of engineered cellular metabolism for microbial chemical production. NATURE[J]. 2018, 555(7698): 683-+, https://www.webofscience.com/wos/woscc/full-record/WOS:000428617600054.
[17] Zhang, Yanfei, Avalos, Jose L. Traditional and novel tools to probe the mitochondrial metabolism in health and disease. WILEY INTERDISCIPLINARY REVIEWS-SYSTEMS BIOLOGY AND MEDICINE. 2017, 9(2): https://www.webofscience.com/wos/woscc/full-record/WOS:000394898500006.
[18] Zhang Yanfei. The quinone-binding site of Acidithiobacillus ferrooxidans sulfide:quinone oxidoreductase controls both sulfide oxidation and quinone reduction.. Biochemistry and Cell Biology. 2015,
[19] Zhang, Yanfei, Weiner, Joel H. A simple semi-quantitative in vivo method using H2S detection to monitor sulfide metabolizing enzymes. BIOTECHNIQUES[J]. 2014, 57(4): 208-210, https://www.webofscience.com/wos/woscc/full-record/WOS:000343303600007.
[20] Zhang, Yanfei, Weiner, Joel H. Characterization of the kinetics and electron paramagnetic resonance spectroscopic properties of Acidithiobacillus ferrooxidans sulfide:quinone oxidoreductase (SQR). ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS[J]. 2014, 564: 110-119, https://www.webofscience.com/wos/woscc/full-record/WOS:000346222600013.
[21] Zhang, Yanfei, Yang, Yu, Liu, Jianshe, Qiu, Guanzhou. Isolation and characterization of Acidithiobacillus ferrooxidans strain QXS-1 capable of unusual ferrous iron and sulfur utilization. HYDROMETALLURGY[J]. 2013, 136: 51-57, http://dx.doi.org/10.1016/j.hydromet.2013.03.005.
[22] Zhang, Yanfei, Peng, Anan, Yang, Yu, Liu, Jianshe, Qiu, Guanzhou. Isolation, characterization of Acidiphilium sp DX1-1 and ore bioleaching by this acidophilic mixotrophic organism. TRANSACTIONS OF NONFERROUS METALS SOCIETY OF CHINA[J]. 2013, 23(6): 1774-1782, https://www.webofscience.com/wos/woscc/full-record/WOS:000324003500035.
[23] Cherney, Maia M, Zhang, Yanfei, James, Michael N G, Weiner, Joel H. Structure-activity characterization of sulfide:quinone oxidoreductase variants. JOURNAL OF STRUCTURAL BIOLOGY[J]. 2012, 178(3): 319-328, https://www.webofscience.com/wos/woscc/full-record/WOS:000305052200011.
[24] Cherney, Maia M, Zhang, Yanfei, Solomonson, Matthew, Weiner, Joel H, James, Michael N G. Crystal Structure of Sulfide:Quinone Oxidoreductase from Acidithiobacillus ferrooxidans: Insights into Sulfidotrophic Respiration and Detoxification. JOURNAL OF MOLECULAR BIOLOGY[J]. 2010, 398(2): 292-305, http://dx.doi.org/10.1016/j.jmb.2010.03.018.
[25] Li Zhang, Ling Tan, Meizi Liu, Yunhong Chen, Yu Yang, Yanfei Zhang, Guoping Zhao. Quantitative measurement of cell-surface displayed proteins based on split-GFP assembly. MICROBIAL CELL FACTORIES. 23(1): http://dx.doi.org/10.1186/s12934-024-02386-1.
科研活动
科研项目
（ 1 ） 多组学定量解析细胞生长与代谢动力学, 负责人, 国家任务, 2020-11--2025-10
（ 2 ） 微生物法稀有金属的回收和超高纯制备, 负责人, 地方任务, 2020-07--2022-07
（ 2 ） 微生物法稀有金属的回收和超高纯制备, 负责人, 地方任务, 2020-07--2022-07
参与会议
（1）Genetically Encoded Biosensor for Branched-Chain Higher Alcohol Production in Saccharomyces cerevisiae 2019-10-20
（2）Genetically encoded Biosensor for Engineering Branched-Chain Higher Alcohol production pathway in Saccharomyces cerevisiae 2019-09-15
（3）Characterization, Structure and Mechanism of Sulfide:quinone oxidoreductase (SQR) from Acidithiobacillus ferrooxidans 2011-09-18
（2）Genetically encoded Biosensor for Engineering Branched-Chain Higher Alcohol production pathway in Saccharomyces cerevisiae 2019-09-15
（3）Characterization, Structure and Mechanism of Sulfide:quinone oxidoreductase (SQR) from Acidithiobacillus ferrooxidans 2011-09-18
指导学生
现指导学生
李峥峥 硕士研究生 071010-生物化学与分子生物学
闫军军 博士研究生 071010-生物化学与分子生物学
肖琪 硕士研究生 086000-生物与医药