0703Z1-化学生物学
083100-生物医学工程

高通量蛋白进化
天然酶的高效鉴定以及底物选择性和活性的高效进化
生物体蛋白的有益优化

2010-09--2015-07   北京大学   研究生博士学位
2006-09--2010-07   北京大学   本科学士学位

   

2015-11~2020-04,美国西北大学, 任职博士后

   

（ 1 ） 一种碳纳米管和金属纳米颗粒复合物及其合成方法, 实用新型, 2011, 第 5 作者, 专利号: ZL200810226843.8
（ 2 ） 4-巯基苯硼酸修饰的金纳米粒子及用其检测细胞表面糖标记的方法, 发明, 2016, 第 4 作者, 专利号: ZL201210342478.3

   

[1] Anming Yang, Huanyu Tao, Lindsey C. Szymczak, Liang Lin, Junfeng Song, Yi Wang, Silei Bai, Justin Modica, Sheng-You Huang, Milan Mrksich, Xinxin Feng. Efficient Enzymatic Incorporation of Dehydroalanine Based on SAMDI-Assisted Identification of Optimized Tags for OspF/SpvC. ACS Chemical Biology[J]. 2022, https://pubs.acs.org/doi/full/10.1021/acschembio.1c00866.

[2] Tongxiao Cui, Yi Man, Feifei Wang, Shuyang Bi, Liang Lin, Ran Xie. Glycoenzyme Tool Development: Principles, Screening Methods, and Recent Advances. Chinese Journal of Chemistry[J]. 2021, https://onlinelibrary.wiley.com/doi/full/10.1002/cjoc.202100770.

[3] Liang Lin, Weston Kightlinger, Sunaina Kiran Prabhu, Adam J Hockenberry, Chao Li, LaiXi Wang, Michael C Jewett, Milan Mrksich. Sequential Glycosylation of Proteins with Substrate-Specific N-Glycosyltransferases. ACS CENTRAL SCIENCE[J]. 2020, 6(2): 144-154, https://www.webofscience.com/wos/woscc/full-record/WOS:000517832800009.

[4] Schumann, Benjamin, Malaker, Stacy Alyse, Wisnovsky, Simon Peter, Debets, Marjoke Froukje, Agbay, Anthony John, Fernandez, Daniel, Wagner, Lauren Jan Sarbo, Lin, Liang, Li, Zhen, Choi, Junwon, Fox, Douglas Michael, Peh, Jessie, Gray, Melissa Anne, Pedram, Kayvon, Kohler, Jennifer Jean, Mrksich, Milan, Bertozzi, Carolyn Ruth. Bump-and-Hole Engineering Identifies Specific Substrates of Glycosyltransferases in Living Cells. MOLECULAR CELL[J]. 2020, 78(5): 824-+, http://dx.doi.org/10.1016/j.molcel.2020.03.030.

[5] Techner, JoseMarc, Kightlinger, Weston, Lin, Liang, Hershewe, Jasmine, Ramesh, Ashvita, DeLisa, Matthew P, Jewett, Michael C, Mrksich, Milan. High-Throughput Synthesis and Analysis of Intact Glycoproteins Using SAMDI-MS. ANALYTICAL CHEMISTRY[J]. 2020, 92(2): 1963-1971, https://www.webofscience.com/wos/woscc/full-record/WOS:000509420400043.

[6] Weston Kightlinger, Katherine E Duncker, Ashvita Ramesh, Ariel H Thames, Aravind Natarajan, Jessica C Stark, Allen Yang, Liang Lin, Milan Mrksich, Matthew P DeLisa, Michael C Jewett. A cell-free biosynthesis platform for modular construction of protein glycosylation pathways. Nature Communications[J]. 2019, 10(1): 1-13, https://doaj.org/article/3ed28a4885534b4f9dbe3db52b4d222c.

[7] Kightlinger, Weston, Lin, Liang, Rosztoczy, Madisen, Li, Wenhao, DeLisa, Matthew P, Mrksich, Milan, Jewett, Michael C. Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases. NATURE CHEMICAL BIOLOGY[J]. 2018, 14(6): 627-+, https://www.webofscience.com/wos/woscc/full-record/WOS:000435445100020.

[8] Hong, Senlian, Lin, Liang, Xiao, Min, Chen, Xing. Live-cell bioorthogonal Raman imaging. CURRENT OPINION IN CHEMICAL BIOLOGY[J]. 2015, 24: 91-96, http://cas-ir.dicp.ac.cn/handle/321008/167366.

[9] Lin Liang. Carbon nanotube-assisted optical activation of TGF-β signalling by near-infrared light. Nature Nanotechnology. 2015, 
[10] Xiao, Ming, Lin, Liang, Li, Zefan, Liu, Jie, Hong, Senlian, Li, Yaya, Zheng, Meiling, Duan, Xuanming, Chen, Xing. SERS Imaging of Cell-Surface Biomolecules Metabolically Labeled with Bioorthogonal Raman Reporters. CHEMISTRY-AN ASIAN JOURNAL[J]. 2014, 9(8): 2040-2044, http://www.irgrid.ac.cn/handle/1471x/944878.

[11] Lin, Liang, Tian, Xiangdong, Hong, Senlian, Dai, Peng, You, Qiancheng, Wang, Ruyi, Feng, Lianshun, Xie, Can, Tian, ZhongQun, Chen, Xing. A Bioorthogonal Raman Reporter Strategy for SERS Detection of Glycans on Live Cells. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION[J]. 2013, 52(28): 7266-7271, https://www.webofscience.com/wos/woscc/full-record/WOS:000321298800044.

[12] Chu, Haibin, Shen, Yihong, Lin, Liang, Qin, Xiaojun, Feng, Ge, Lin, Ziyin, Wang, Jinyong, Liu, Haichao, Li, Yan. Ionic-Liquid-Assisted Preparation of Carbon Nanotube-Supported Uniform Noble Metal Nanoparticles and Their Enhanced Catalytic Performance. ADVANCED FUNCTIONAL MATERIALS[J]. 2010, 20(21): 3747-3752, http://dx.doi.org/10.1002/adfm.201001240.

[13] Du Jun, Hong Senlian, Dong Lu, Cheng Bo, Lin Liang, Zhao Bing, Chen Ye-Guang, Chen Xing. Dynamic Sialylation in Transforming Growth Factor-β (TGF-β)-induced Epithelial to Mesenchymal Transition *. The Journal of Biological Chemistry. 290(19): 12000-12013, http://dx.doi.org/10.1074/jbc.M115.636969.

   

（ 1 ） 高通量进化方法, 主持, 部委级, 2020-06--2025-05
（ 2 ） 酶的高通量进化, 主持, 部委级, 2020-06--2030-06

（1）Parallel screening and rapid identification of orthogonal glycosyltransferases   2019-10-04
（2）Parallel screening and rapid identification of orthogonal tetra-N-glycosyltransferases for glycoprotein engineering   2019-09-12
（3）Site-specific glycosylation of protein via N-glycosyltransferases with substrate orthogonality   2019-07-29
（4）Engineering N-glycosyltransferases for whole substrate library with high-throughput mass spectrometry   2019-07-19
（5）Quantification analysis in site-directed mutagenesis of N-glycosyltransferases for peptide substrate library preference   2018-11-16