071010-生物化学与分子生物学
086000-生物与医药

蛋白质工程，酶和生物催化，合成生物学
合成生物学，生物工程

1999-09--2002-07   中国科学院微生物研究所   博士

   

2015-07~现在, 中国科学院青岛生物能源与过程研究所, 副研究员
2008-08~2015-03,Imperial College London, UK, Senior Research Associate
2004-10~2007-12,Imperial College London, UK, Research Associate
2002-08~2004-10,纽英伦生物技术（北京）有限公司, 研发部主任兼生产部经理

2018-04-16-今,EDITORIAL BOARD MEMBER of American Journal of Bioscience and Bioengineering (BIO), EDITORIAL BOARD MEMBER

   

（1） 最佳展板奖, 其他, 2018
（2） Best poster prize, The 18th CLSS-UK Annual Meeting. London UK, 其他, 2012
（3） Best poste prize, The XXIInd FECTS Meeting. Davos, Swiss, 其他, 2010
（4） Conference Attendance Award, ICGEB (International Centre for Genetic Engineering and Biotechnology), 其他, 2007

（ 1 ） 一种对甘油葡糖苷溶液进行脱色的方法, 发明专利, 2024, 第 3 作者, 专利号: 2024103333522

（ 2 ） 一种用于纯化甘油葡糖苷的方法, 发明专利, 2024, 第 3 作者, 专利号: 2024103338206

（ 3 ） 新型R型ω-转氨酶及其应用, 发明专利, 2023, 第 12 作者, 专利号: CN116515788A

（ 4 ） 一种酶法合成甘油葡糖苷反应液的过滤纯化系统, 实用新型, 2022, 第 3 作者, 专利号: CN217247358U

（ 5 ） 一种生物酶法催化合成(R)-2-(1-氨乙基)-4-氟苯酚的方法, 发明专利, 2022, 第 3 作者, 专利号: 202210663645.8

（ 6 ） 一种高效蔗糖磷酸化酶在制备甘油葡糖苷中的应用, 发明专利, 2022, 第 2 作者, 专利号: 202210479826.5

（ 7 ） 一种高效蔗糖磷酸化酶及其在生产甘油葡糖苷中的应用, 发明专利, 2022, 第 2 作者, 专利号: 202210479829.9

（ 8 ） 一种四步法分离纯化甘油葡糖苷的方法, 发明专利, 2022, 第 3 作者, 专利号: 202210479025.9

（ 9 ） 一种高效生产甘油葡糖苷的蔗糖磷酸化酶及其应用, 发明专利, 2022, 第 2 作者, 专利号: 202210479024.4

（ 10 ） 一种二步法分离纯化甘油葡糖苷的方法, 发明专利, 2022, 第 3 作者, 专利号: 202210479828.4

（ 11 ） 一种高效蔗糖磷酸化酶及其在生产甘油葡糖苷中的应用, 发明专利, 2022, 第 2 作者, 专利号: 202210479827.X

（ 12 ） 一种耐热蔗糖磷酸化酶在生产甘油葡糖苷中的应用, 发明专利, 2022, 第 2 作者, 专利号: 202210479136.X

（ 13 ） 一种甘油葡糖苷生产方法, 发明专利, 2022, 第 3 作者, 专利号: 202210479830.1

（ 14 ） α-烯烃的生产, 发明专利, 2018, 专利号: CN108779476A

   

[1] Quan Luo, Guan Zhou, Zhongxia Li, Jiangpeng Dong, Hang Zhao, Huifang Xu, Xuefeng Lu. ω-transaminase-catalyzed synthesis of (R)-2-(1-aminoethyl)-4-fluorophenol, a chiral intermediate of novel anti-tumor drugs. ENZYME AND MICROBIAL TECHNOLOGY. 2024, 第 6 作者  通讯作者  175: http://dx.doi.org/10.1016/j.enzmictec.2024.110406.
[2] Kong, Fanhui, Chen, Jie, Qin, Xiangquan, Liu, Chuanfei, Jiang, Yiping, Ma, Li, Xu, Huifang, Li, Shengying, Cong, Zhiqi. Evolving a P450BM3 Peroxygenase for the Production of Indigoid Dyes from Indoles. CHEMCATCHEM[J]. 2022, 第 7 作者  通讯作者  14(24): 
[3] Xu, Huifang, Liang, Weinan, Ning, Linlin, Jiang, Yuanyuan, Yang, Wenxia, Wang, Cong, Qi, Feifei, Ma, Li, Du, Lei, Fourage, Laurent, Zhou, Yongjin J, Li, Shengying. Directed Evolution of P450 Fatty Acid Decarboxylases via High-Throughput Screening towards Improved Catalytic Activity. CHEMCATCHEM[J]. 2020, 第 1 作者12(1): 80-84, https://www.webofscience.com/wos/woscc/full-record/WOS:000492792500001.
[4] Yuanyuan Jiang, Zhong Li, Shanmin Zheng, Huifang Xu, Yongjin J. Zhou, Zhengquan Gao, Chunxiao Meng, Shengying Li. Establishing an enzyme cascade for one-pot production of α-olefins from low-cost triglycerides and oils without exogenous H2O2 addition. BIOTECHNOLOGY FOR BIOFUELS[J]. 2020, 第 4 作者13(1): 1-11, 
[5] Jiang, Yuanyuan, Li, Zhong, Wang, Cong, Zhou, Yongjin J, Xu, Huifang, Li, Shengying. Biochemical characterization of three new alpha-olefin-producing P450 fatty acid decarboxylases with a halophilic property. BIOTECHNOLOGY FOR BIOFUELS[J]. 2019, 第 5 作者  通讯作者  12: 79, http://cas-ir.dicp.ac.cn/handle/321008/165618.
[6] Fang, Bo, Xu, Huifang, Liu, Yi, Qi, Fengxia, Zhang, Wei, Chen, Hui, Wang, Cong, Wang, Yilin, Yang, Wenxia, Li, Shengying. Mutagenesis and redox partners analysis of the P450 fatty acid decarboxylase OleT(JE). SCIENTIFIC REPORTS[J]. 2017, 第 2 作者7: http://ir.qibebt.ac.cn/handle/337004/9344.
[7] Xu, Huifang, Ning, Linlin, Yang, Wenxia, Fang, Bo, Wang, Cong, Wang, Yun, Xu, Jian, Collin, Severine, Laeuffer, Frederic, Fourage, Laurent, Li, Shengying. In vitro oxidative decarboxylation of free fatty acids to terminal alkenes by two new P450 peroxygenases. BIOTECHNOLOGY FOR BIOFUELS[J]. 2017, 第 1 作者10(1): http://dx.doi.org/10.1186/s13068-017-0894-x.
[8] Li, Zhong, Du, Lei, Zhang, Wei, Zhang, Xingwang, Jiang, Yuanyuan, Liu, Kun, Men, Ping, Xu, Huifang, Fortman, Jeffrey L, Sherman, David H, Yu, Bing, Gao, Song, Li, Shengying. Complete elucidation of the late steps of bafilomycin biosynthesis in Streptomyces lohii. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2017, 第 8 作者292(17): 7095-7104, http://dx.doi.org/10.1074/jbc.M116.751255.
[9] 孙月, 马莉, 陈辉, 徐卉芳, 郑贤良, 齐凤霞, 李盛英. 脂肪酶-P450脂肪酸脱羧酶融合蛋白催化的a烯烃合成. 科学通报[J]. 2017, 第 4 作者62(31): 3576-3582, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=6145690&detailType=1.
[10] An, Bo, Abbonante, Vittorio, Xu, Huifang, Gavriilidou, Despoina, Yoshizumi, Ayumi, Bihan, Dominique, Farndale, Richard W, Kaplan, David L, Balduini, Alessandra, Leitinger, Birgit, Brodsky, Barbara. Recombinant Collagen Engineered to Bind to Discoidin Domain Receptor Functions as a Receptor Inhibitor. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2016, 第 3 作者291(9): 4343-4355, http://dx.doi.org/10.1074/jbc.M115.674507.
[11] 徐卉芳. Molecular and cellular analysis of a novel mutation in DDR2 causing Spondylo-meta-epiphyseal dysplasia with short limbs and abnormal calcifications (SMED-SL). BMC Medical Genetics. 2014, 第 1 作者
[12] Xu Huifang, Abe Takemoto, Liu Justin K H, Zalivina Irina, Hohenester Erhard, Leitinger Birgit. Normal activation of discoidin domain receptor 1 mutants with disulfide cross-links, insertions, or deletions in the extracellular juxtamembrane region: mechanistic implications.. THE JOURNAL OF BIOLOGICAL CHEMISTRY. 2014, 第 1 作者http://kns.cnki.net/KCMS/detail/detail.aspx?QueryID=0&CurRec=1&recid=&FileName=SJPDD0238FFA03FFDC777812A2CD519ECDEB&DbName=SJPD_06&DbCode=SJPD&yx=&pr=&URLID=&bsm=.
[13] Xu, Huifang, Bihan, Dominique, Chang, Francis, Huang, Paul H, Farndale, Richard W, Leitinger, Birgit. Discoidin Domain Receptors Promote alpha 1 beta 1-and alpha 2 beta 1-Integrin Mediated Cell Adhesion to Collagen by Enhancing Integrin Activation. PLOS ONE[J]. 2012, 第 1 作者7(12): https://www.webofscience.com/wos/woscc/full-record/WOS:000312794500114.
[14] Xu, Huifang, Raynal, Nicolas, Stathopoulos, Stavros, Myllyharju, Johanna, Farndale, Richard W, Leitinger, Birgit. Collagen binding specificity of the discoidin domain receptors: Binding sites on collagens II and III and molecular determinants for collagen IV recognition by DDR1. MATRIX BIOLOGY[J]. 2011, 第 1 作者30(1): 16-26, http://dx.doi.org/10.1016/j.matbio.2010.10.004.
[15] Ali, Bassam R, Xu, Huifang, Akawi, Nadia A, John, Anne, Karuvantevida, Noushad S, Langer, Ruth, AlGazali, Lihadh, Leitinger, Birgit. Trafficking defects and loss of ligand binding are the underlying causes of all reported DDR2 missense mutations found in SMED-SL patients. HUMAN MOLECULAR GENETICS[J]. 2010, 第 2 作者19(11): 2239-2250, https://www.webofscience.com/wos/woscc/full-record/WOS:000277448500013.
[16] Xu, HF, Zhang, XE, Zhang, ZP, Zhang, YM, Cass, AEG. Directed evolution of E-coli alkaline phosphatase towards higher catalytic activity. BIOCATALYSIS AND BIOTRANSFORMATION[J]. 2003, 21(1): 41-47, http://www.corc.org.cn/handle/1471x/2376138.
[17] Zhang, XE, Zhou, YH, Zhang, ZP, Xu, HF, Shao, WH, Cass, AEG. Engineering E-coli alkaline phosphatase yields changes of catalytic activity, thermal stability and phosphate inhibition. BIOCATALYSIS AND BIOTRANSFORMATION[J]. 2002, 20(6): 381-389, http://www.corc.org.cn/handle/1471x/2376108.
[18] 徐卉芳, 牛钟相. 牛消化道正常菌群的研究. 山东畜牧兽医[J]. 1999, 第 1 作者39-44, http://lib.cqvip.com/Qikan/Article/Detail?id=3671319.
[19] 徐卉芳, 牛钟相. 肉牛粪便正常菌群研究. 黑龙江畜牧兽医[J]. 1999, 第 1 作者3-4, http://lib.cqvip.com/Qikan/Article/Detail?id=3489618.

   

（ 1 ） Improving C12 Activity of New P450 Decarboxylase Encoding Sequences, 负责人, 境内委托项目, 2015-07--2017-02
（ 2 ） 基于P450催化元件的高值天然产物生物合成途径重构, 参与, 地方任务, 2017-08--2019-07
（ 3 ） Proof of Concept of Pathway Integration for α-Olefin Biosynthesis in Photosynthetic Microorganisms, 负责人, 境内委托项目, 2017-07--2019-07
（ 4 ） P450 脂肪酸脱羧酶的定向进化及催化机理研究, 负责人, 国家任务, 2018-01--2021-12
（ 5 ） 耦合非天然辅酶的P450脂肪酸衍生细胞工厂, 负责人, 研究所自主部署, 2018-03--2020-02
（ 6 ） 类囊体膜介导的光驱α-烯烃生物合成研究, 负责人, 研究所自主部署, 2020-01--2021-12
（ 7 ） 中科斯芬克司药物合成特种酶联合实验室项目, 负责人, 境内委托项目, 2021-01--2026-01
（ 8 ） 临床药用蛋白的酶工程改造研究, 负责人, 地方任务, 2024-01--2026-12

（1）Directed Evolution of P450 Fatty Acid Decarboxylases via High-Throughput Screening towards Improved Catalytic Activity   2019-10-20
（2）催化脂肪酸脱羧生成末端烯烃的新型P450过氧化物酶的鉴定和酶学表征   2018年中国微生物学会学术年会   2018-10-19
（3）Characterization of novel P450 fatty acids decarboxylases for 1-alkene production   第三届合成微生物学与生物制造暨第八届生物催化与生物合成工程国际学术研讨会   2018-10-12
（4）Characterization of novel P450 peroxygenases for oxidative decarboxylation of free fatty acids to terminal alkenes   2018-06-30
（5）学术交流   全国首届生物传感、生物芯片高端论坛   2017-10-20
（6）学术交流   国际化学生物学年会   2017-10-17
（7）学术交流   2016-10-16
（8）Understanding the molecular mechanism for discoidin domain receptor activation   2013-09-01
（9）Discoidin Domain Receptors Promote β1-integrin Mediated Cell Adhesion to Collagen   2012-09-07
（10）The role of discoidin domain receptors in cell adhesion   2012-04-02
（11）The role of DDR Receptors in cell adhesion   2011-09-05
（12）Trafficking defects and loss of ligand binding are the underlying causes of all reported DDR2 missense mutations found in SMED-SL patients   2010-07-03
（13）Determining the collagen binding specificity of the discoidin domain receptors (DDRs)   2010-03-29
（14）Characterization of polyomavirus middle T antigen complexes using affinity purification and MS   2007-07-22

已指导学生

宁琳琳  硕士研究生  085238-生物工程  

马娜娜  硕士研究生  085238-生物工程  

梁伟男  硕士研究生  071010-生物化学与分子生物学