Chromatographic separation; proteomic analysis

2011.07 Received Ph. D degree from Dalian Institute of Chemical Physics, Graduate University of Chinese Academy of Sciences, China

2005.09-2011.07 Studied in Dalian Institute of Chemical Physics, Graduate University of Chinese Academy of Sciences, China, majoring in Analytical Chemistry under the supervision of Prof. Yukui Zhang and Prof. Lihua Zhang

2005.07  Received Bachelor’s degree from Wuhan University, China 

  2001.09-2005.07 Studied in Wuhan University, China, majoring in Applied Chemistry

2018.10-present   Work as Associate Professor, doing the research in the group of Prof. Yukui Zhang and Lihua Zhang, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, china

2017.10-2018.10   Work as visiting scholar, doing the research in the group of Prof. Ying Ge, University of Wisconsin-Madison, United States

2014.07-2017.10   Work as Associate Professor, doing the research in the group of Prof. Yukui Zhang and Lihua Zhang, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, china

2011.07-2014.07   Worked as Assistant professor, doing the research in the group of Prof. Yukui Zhang and Lihua Zhang, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, china

(1) Yue Sun, Dan Liu, Yu Liang*, Xue Yang, Xinxin Liu, Baofeng Zhao, Zhen Liang, Yukui Zhang, Lihua Zhang*. High-Throughput Proteoform Imaging for Revealing Spatial-Resolved Changes in Brain Tissues Associated with Alzheimer's Disease, Adv. Sci. 2025, e2416722. doi: 10.1002/advs.202416722.

(2)  Yue Sun, Yu Liang*, Chao Wang, Baofeng Zhao, Zhen Liang, Xiaodan Zhang, Yukui Zhang, Lihua Zhang*, Enhancing Top-Down Characterization of Membrane Proteoforms with C8-Functional Amine-Bridged Hybrid Monoliths, Anal. Chem., 2023, 95(17):6846-6853.

(3)  Yu Liang, Lihua Zhang, Yukui Zhang, Chromatographic separation of peptides and proteins for characterization of proteomes, Chem. Commun., 2023, 59(3):270-281.

(4)  Chao Wang, Yu Liang*, Xue Yang, Bowen Zhong, Xiaodan Zhang, Baofeng Zhao, Zhen Liang, Lihua Zhang*, Yukui Zhang, Surface-Charged Hybrid Monolithic Column for MS-Compatible Peptide Separation with High Peak Capacity and Its Application in Proteomic Analysis, Anal. Chem., 2022, 94(27):9525-9529.

(5)  Yu Liang, Lihua Zhang, Yukui Zhang, Monolithic Materials-Based RPLC-MS for Proteoform Separation and Identification, Methods Mol Biol. 2022;2500:43-53.

(6)  Chao Wang, Yu Liang*, Baofeng Zhao, Zhen Liang, Lihua Zhang*, Yukui Zhang, Ethane-Bridged Hybrid Monolithic Column with Large Mesopores for Boosting Top-Down Proteomic Analysis, Anal. Chem., 2022, 94(16):6172-6179.

(7)  Yu Liang, Chao Wang, Zhen Liang, Lihua Zhang, Yukui Zhang, C18-Functionalized Amine-Bridged Hybrid Monoliths for Mass Spectrometry-Friendly Peptide Separation and Highly Sensitive Proteomic Analysis, Anal. Chem., 2022, 94(16):6084-6088.

(8)  Yu Liang, Lihua Zhang*, Yukui Zhang, Well-Defined Materials for High-Performance Chromatographic Separation, Annu. Rev. Anal. Chem., 2019, 12(1):451-473.

(9)  Yu Liang^#, Yutong Jin^#, Zhijie Wu, Trisha Tucholski, Kyle A. Brown, Lihua Zhang*, Yukui Zhang, Ying Ge*, Bridged Hybrid Monolithic Column Coupled to High-Resolution Mass Spectrometry for Top-Down Proteomics, Anal. Chem., 2019, 91(3):1743-1747.

(10) Ci Wu^#, Yu Liang^#, Xudong Zhu, Qun Zhao, Fei Fang, Xiaodan Zhang, Zhen Liang, Lihua Zhang*, Yukui Zhang, Macro-mesoporous organosilica monoliths with bridged-ethylene and terminal-vinyl: High-density click functionalization for chromatographic separation, Anal. Chim. Acta. 2018, 1038:198-205. 

(11) Ci Wu^#, Yu Liang^#, Zhen Liang, Lihua Zhang*, Yukui Zhang, Ethane-bridged hybrid monoliths with well-defined mesoporosity and great stability for high-performance peptide separation, Anal. Chim. Acta. 2018, 1019:128-134. 

(12) Xudong Zhu^#, Yu Liang^#, Yejing Weng, Yuanbo Chen, Hao Jiang, Lihua Zhang*, Zhen Liang, Yukui Zhang, Gold-Coated Nanoelectrospray Emitters Fabricated by Gravity-Assisted Etching Self-Termination and Electroless Deposition, Anal Chem., 2016, 88, 11347-11351. 

(13) Ci Wu^#, Yu Liang^#, Kaiguang Yang, Yi Min, Zhen Liang, Lihua Zhang*, Yukui Zhang, Clickable Periodic Mesoporous Organosilica Monolith for Highly Efficient Capillary Chromatographic Separation, Anal. Chem., 2016, 88, 1521-1525. 

(14) Yu Liang^#, Ci Wu^#, Qun Zhao, Qi Wu, Bo Jiang, Yejing Weng, Zhen Liang, Lihua Zhang*, Yukui Zhang, Gold nanoparticles immobilized hydrophilic monoliths with variable functional modification for highly selective enrichment and on-line deglycosylation of glycopeptides. Anal. Chim. Acta., 2015, 900, 83-89.

(15) Ci Wu^#, Yu Liang^#, Qun Zhao, Yanyan Qu, Shen Zhang, Qi Wu, Zhen Liang, Lihua Zhang*, Yukui Zhang, Gold nanoparticles immobilized hydrophilic monoliths with variable functional modification for highly selective enrichment and on-line deglycosylation of glycopeptides, Chemistry-A European Journal, 2014, 20, 8737-8743. 

(16) Yu Liang, Lihua Zhang*, Yukui Zhang, Recent advances in monolithic columns for protein and peptide separation by capillary liquid chromatography, Anal. Bioanal. Chem., 2013, 405, 2095-2106.

(17) Yu Liang, Guijie Zhu, Tingting Wang, Xiaodan Zhang, Zhen Liang, Lihua Zhang*, Yukui Zhang, Fast preparation of monolithic immobilized pH gradient column by photopolymerization and photografting techniques for isoelectric focusing separation of proteins, Electrophoresis, 2011, 32, 2911-2914.

(18) Yu Liang, Dingyin Tao, Junfeng Ma, Liangliang Sun, Zhen Liang, Lihua Zhang*, Yukui Zhang, Hydrophilic monolith based immobilized enzyme reactors in capillary and on microchip for high-throughput proteomic analysis, J. Chromatogr. A, 2011, 1218, 2898-2905.

(19) Yu Liang, Yongzheng Cong, Zhen Liang, Lihua Zhang*, Yukui Zhang, Microchip isoelectric focusing with monolithic immobilized pH gradient materials for proteins separation, Electrophoresis, 2009, 30, 4034-4039.