基本信息
吴边  男  博导  中国科学院微生物研究所
电子邮件: wub@im.ac.cn
通信地址: 北京市朝阳区北辰西路1号院3号中科院微生物研究所F411
邮政编码: 100101

招生信息

   
招生专业
100705-微生物与生化药学
招生方向
计算机蛋白质设计,绿色化学,生物化工

教育背景

2006-09--2010-10   荷兰格罗宁根大学   博士研究生
2004-09--2006-07   荷兰格罗宁根大学   硕士研究生
2000-09--2004-07   北京大学   本科 学士

工作经历

   
工作简历
2017-12~现在, 中国科学院微生物研究所 微生物前期资源开发国家重点实验室, 副主任
2014-12~现在, 中国科学院微生物研究所 微生物生理与代谢工程重点实验室, 研究员
2012-10~2014-11,荷兰格罗宁根大学, 研究组长 主任生物学家
2010-11~2012-09,荷兰格罗宁根大学, 博士后
2006-09~2010-10,荷兰格罗宁根大学, 博士研究生
2004-09~2006-07,荷兰格罗宁根大学, 硕士研究生
2000-09~2004-07,北京大学, 本科 学士
社会兼职
2021-10-01-今,中国微生物学会, 常务理事
2021-10-01-今,mLife, 编委
2020-01-01-今,合成生物学, 编委
2018-11-11-今,中国生物工程学会合成生物学专业委员会, 委员
2017-08-01-今,中国生物化学与分子生物学学会, 理事
2016-12-01-今,北京生物化学学会, 理事
2016-06-09-今,中国生物发酵产业协会, 理事
2016-04-13-今,中国微生物学会酶工程专业委员会委员, 委员
2015-09-01-今,中国食品科学技术学会, 理事

教授课程

天然产物药物的生物合成及生物催化
代谢工程

专利与奖励

   
专利成果
( 1 ) 一种酶催化的蛋白质C-端选择性酰肼化修饰方法, 2021, 第 1 作者, 专利号: CN112391429A

( 2 ) 一种酶催化的多肽C-端选择性酰胺化和酰肼化修饰方法, 2020, 第 1 作者, 专利号: CN112094879A

( 3 ) 一种筛选能将L-氨基酸转化为2-酮酸酶的方法, 2020, 第 1 作者, 专利号: CN111951898A

( 4 ) 一种具有高稳定性的重组纤维素酶MaCel, 2020, 第 1 作者, 专利号: CN111004793A

( 5 ) 一种氨基裂解酶突变体蛋白及其编码基因与应用, 2020, 第 2 作者, 专利号: CN108866028B

出版信息

   
发表论文
(1) Computational Redesign of a PETase for Plastic Biodegradation under Ambient Condition by the GRAPE Strategy, ACS CATALYSIS, 2021, 通讯作者
(2) Development of a versatile and efficient C-N lyase platform for asymmetric hydroamination via computational enzyme redesign, NATURE CATALYSIS, 2021, 通讯作者
(3) Construction of an Alternative NAD(+) De Novo Biosynthesis Pathway, ADVANCED SCIENCE, 2021, 第 9 作者
(4) Characterization and efficient production of a thermostable, halostable and organic solvent-stable cellulase from an oil reservoir, INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, 通讯作者
(5) Bioretrosynthesis of Functionalized N ‐Heterocycles from Glucose via One‐Pot Tandem Collaborations of Designed Microbes, ADVANCED SCIENCE, 2020, 通讯作者
(6) Improving the System Performance of the Asymmetric Biosynthesis of D-Pantoic Acid by Using Artificially Self-Assembled Enzymes in Escherichia coli, ACS BIOMATERIALS SCIENCE & ENGINEERING, 2020, 通讯作者
(7) Reductase of Mutanobactin Synthetase Triggers Sequential C-C Macrocyclization, C-S Bond Formation, and C-C Bond Cleavage, ORGANIC LETTERS, 2020, 第 8 作者
(8) Thermostability improvement of the glucose oxidase from Aspergillus niger for efficient gluconic acid production via computational design, INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2019, 通讯作者
(9) Molecular dynamics investigations of structural and functional changes in Bcl-2 induced by the novel antagonist BDA-366, JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2019, 通讯作者
(10) 合成微生物组:当“合成生物学”遇见“微生物组学”, Synthetic microbiome: When "synthetic biology" meets "microbiomics", 科学通报, 2019, 第 2 作者
(11) 蛋白质工程:从定向进化到计算设计, Protein engineering:from directed evolution to computational design, 生物工程学报, 2019, 第 4 作者
(12) Biochemical and structural characterization of a highly active branched-chain amino acid aminotransferase from Pseudomonas sp. for efficient biosynthesis of chiral amino acids, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2019, 通讯作者
(13) Molecular dynamics investigations of structural and functional changes in Bcl-2 induced by the novel antagonist BDA-366, JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2019, 通讯作者
(14) Biochemical and structural characterization of a highly active branched-chain amino acid aminotransferase from Pseudomonas sp. for efficient biosynthesis of chiral amino acid., Appl. Microbiol. Biotech., 2019, 
(15) 符合工程化需求的生物元件设计, Biological Components Design for Engineering Requirements, 中国科学院院刊, 2018, 第 2 作者
(16) Enzymatic clickable functionalization of peptides via computationally engineered peptide amidase, CHINESE CHEMICAL LETTERS, 2018, 通讯作者
(17) Cryptococcus neoformans sexual reproduction is controlled by a quorum sensing peptide, NATURE MICROBIOLOGY, 2018, 
(18) Enzymatic clickable functionalization of peptides via computationally engineered peptide amidase, Enzymatic clickable functionalization of peptides via computationally engineered peptide amidase, 中国化学快报:英文版, 2018, 第 4 作者
(19) Engineering improved thermostability of the GH11 xylanase from Neocallimastix patriciarum via computational library design, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2018, 通讯作者
(20) Computational redesign of enzymes for regio- and enantioselective hydroamination, NATURE CHEMICAL BIOLOGY, 2018, 通讯作者
(21) Molecular dynamics investigations of structural and functional changes in Bcl-2 induced by the novel antagonist BDA-366., J.Biomol.Struct.&Dyn, 2018, 通讯作者
(22) 计算机辅助蛋白结构预测及酶的计算设计研究进展, A Brief Overview of Computational Protein Structure Prediction and Enzyme Design, 广西科学, 2017, 第 2 作者
(23) Regio- and Stereospecific O-Glycosylation of Phenolic Compounds Catalyzed by a Fungal Glycosyltransferase from Mucor hiemalis, ADVANCED SYNTHESIS & CATALYSIS, 2017, 第 9 作者
(24) Versatile Peptide C-Terminal Functionalization via a Computationally Engineered Peptide Amidase, ACS CATALYSIS, 2016, 通讯作者
(25) Engineering a Diverse Ligase Toolbox for Peptide Segment Condensation, ADVANCED SYNTHESIS & CATALYSIS, 2016, 通讯作者
(26) Enzymatic Network for Production of Ether Amines From Alcohols, BIOTECHNOLOGY AND BIOENGINEERING, 2016, 第 10 作者
(27) Peptiligase, an Enzyme for Efficient Chemoenzymatic Peptide Synthesis and Cyclization in Water, ADVANCED SYNTHESIS & CATALYSIS, 2016, 通讯作者
(28) Peptide synthesis in neat organic solvents with novel thermostable proteases, ENZYME AND MICROBIAL TECHNOLOGY, 2015, 第 4 作者
(29) One-Step C-Terminal Deprotection and Activation of Peptides with Peptide Amidase from Stenotrophomonas maltophilia in Neat Organic Solvent, ADVANCED SYNTHESIS & CATALYSIS, 2014, 通讯作者
(30) Structural Investigations into the Stereochemistry and Activity of a Phenylalanine-2,3-aminomutase from Taxus chinensis, BIOCHEMISTRY, 2014, 第 3 作者
(31) Azobenzene Photoswitches for Staudinger-Bertozzi Ligation, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2013, 第 2 作者
(32) Priming ammonia lyases and aminomutases for industrial and therapeutic applications, CURRENT OPINION IN CHEMICAL BIOLOGY, 2013, 第 2 作者
(33) Proteolysin, a Novel Highly Thermostable and Cosolvent-Compatible Protease from the Thermophilic Bacterium Coprothermobacter proteolyticus, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2013, 第 2 作者
(34) Redesign of a Phenylalanine Aminomutase into a Phenylalanine Ammonia Lyase, CHEMCATCHEM, 2013, 第 5 作者
(35) Biochemical Properties and Crystal Structure of a β-Phenylalanine Aminotransferase from Variovorax paradoxus, Applied and Environmental Microbiology, 2013, 
(36) Mechanism-Inspired Engineering of Phenylalanine Aminomutase for Enhanced ss-Regioselective Asymmetric Amination of Cinnamates, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2012, 第 1 作者
(37) C-N lyases catalyzing addition of ammonia, amines and amides to C=C and C=O bonds, Enzyme Catalysis in Organic Synthesis 3rd edition, Wiley Edition, 2012, 第 1 作者
(38) Preparation of β-phenylalanine using phenylalanine aminomutase, Practical Methods in Biocatalysis and Biotransformations, Wiely Edition, 2012, 第 1 作者
(39) Aminomutases: mechanistic aspects, biotechnological applications and future perspectives, Trends. Biotechnol, 2011, 第 1 作者
(40) Engineering of an enantioselective tyrosine aminomutase by mutation of a single active site residue in phenylalanine aminomutase, CHEMICAL COMMUNICATIONS, 2010, 第 1 作者
(41) Efficient Tandem Biocatalytic Process for the Kinetic Resolution of Aromatic beta-Amino Acids, ADVANCED SYNTHESIS & CATALYSIS, 2010, 第 1 作者
(42) Enantiomerically pure beta-phenylalanine analogues from alpha-beta-phenylalanine mixtures in a single reactive extraction step, CHEMICAL COMMUNICATIONS, 2010, 
(43) Phenylalanine Aminomutase-Catalyzed Addition of Ammonia to Substituted Cinnamic Acids: a Route to Enantiopure alpha- and beta-Amino Acids, JOURNAL OF ORGANIC CHEMISTRY, 2009, 第 2 作者
(44) Enzymatic Synthesis of Enantiopure alpha- and beta-Amino Acids by Phenylalanine Aminomutase-Catalysed Amination of Cinnamic Acid Derivatives, CHEMBIOCHEM, 2009, 第 1 作者

科研活动

   
科研项目
( 1 ) ****, 负责人, 中国科学院计划, 2015-12--2018-12
( 2 ) 衍生库建设-反应酶库, 负责人, 中国科学院计划, 2016-12--2020-06
( 3 ) 基于能量与分子动态的超耐热葡萄糖氧化酶设计, 负责人, 国家任务, 2017-01--2019-12
( 4 ) 生物法生产2甲基5羧基吡嗪的关键技术开发, 负责人, 企业委托, 2016-08--2018-08
( 5 ) 嗜麦芽寡养单胞菌多肽修饰酶催化机理研究与应用, 负责人, 国家任务, 2019-01--2021-12
( 6 ) 微生物催化, 负责人, 国家任务, 2019-01--2021-12
( 7 ) 非天然人工酶的计算设计, 负责人, 中国科学院计划, 2020-01--2024-12
( 8 ) 种子精准设计的变革技术, 负责人, 中国科学院计划, 2019-05--2024-12
( 9 ) 新分子生化反应设计与核心生命途径重构, 负责人, 国家任务, 2019-07--2024-06
参与会议
(1)酶催化碳氮成键反应的计算设计   中国合成生物学学术年会暨第三届工程生物创新大会・光明科学城2021   2021-12-23
(2)微生物工业酶的计算设计   后AlphaFold时代的蛋白质计算预测与设计   2021-09-17
(3)Computational Directed Evolution of Enzymes For Industrially Vibale Reactions   中国科协第383次青年科学家论坛   2019-10-20
(4)Computational Enzyme Design For Industrially Relevant Reactions   第25届酶工程会议   2019-09-14
(5)塑料降解酶的计算工程设计   第十四届全国酶学学术讨论会   2019-08-12
(6)酶催化碳-氮成键反应的计算设计   第十二届中国酶工程学术研讨会   2019-08-09
(7)Computational Enzyme Design For Microbial Catalysis    国际环境和健康微生物学会议暨海外华人微生物学会年会   2019-07-12
(8)Computational enzyme redesign for regio- and enantioselective hydroamination reactions   2018-11-22
(9)Versatile Peptide C‑Terminal Functionalization via a Computationally Engineered Peptide Amidase   2018-07-03
(10)From Zero to Hero: 计算机蛋白质设计时代的来临   第十一届中国酶工程学术研讨会   2017-11-18