1. 利用冷冻电子显微术研究重要蛋白质复合物的结构及功能
2. 发展冷冻电子显微术方法
3. 利用冷冻电子显微术研究病毒的三维结构及病毒入侵、感染细胞以及抗体中和病毒的机制

   

071011-生物物理学

冷冻电镜在生物样品三维结构解析中的应用及其方法发展
利用冷冻电子显微术研究重要蛋白质复合物的结构及功能；发展冷冻电子显微术方法
利用冷冻电子显微术研究病毒的三维结构及病毒入侵、感染细胞以及抗体中和病毒的机制

2003-09--2008-06   北京大学   理学博士
1999-09--2003-06   北京大学   理学学士

   

2014-10~现在, 中国科学院生物物理研究所, 研究员
2009-01~2014-09,美国普渡大学, 博士后

生物大分子的电镜三维结构
结构生物学与医学研究
科研实践Ⅰ-综合物理实验（非课程）
（待删除）生物大分子的电镜三维结构
生物大分子电子显微三维重构

   

（1） 中源协和生命医学奖，创新突破奖, 其他, 2018

[1] 章新政, 李不凡, 孔令斐. 应用界面电荷制备冷冻电镜样品的方法. ZL202110600516.X, 2022-05-31.
[2] 章新政, 朱东杰. 直接电子探测相机的成像方法、装置及计算机设备. CN: CN113074627A, 2021-07-06.
[3] 章新政, 吴春玲, 石会刚. 一种冷冻电镜样品制备方法. CN: CN110823931B, 2020-10-27.

   

[1] Xin You, Xing Zhang, Jing Cheng, Yanan Xiao, ma jianfei, Shan Sun, Zhang, Xinzheng, Hong-Wei Wang, SenFang Sui. In situ structure of the red algal phycobilisome–PSII–PSI–LHC megacomplex. Nature[J]. 2023, [2] Jing Cheng, Tong Liu, Xin You, Fa Zhang, Sen-Fang Sui, Xiaohua Wan, Xinzheng Zhang. Determining protein structures in cellular lamella at pseudo-atomic resolution by GisSPA. NATURE COMMUNICATIONS[J]. 2023, 14(1): 1-9, http://dx.doi.org/10.1038/s41467-023-36175-y.
[3] Duanfang Cao, Bingting Ma, Ziyi Cao, Xinzheng Zhang, Ye Xiang. Structure of Semliki Forest virus in complex with its receptor VLDLR. CELL[J]. 2023, 186(10): 2208-2218.e15, http://dx.doi.org/10.1016/j.cell.2023.03.032.
[4] Qi yang, chunling wu, dongjie zhu, Junxi Li, Jing Cheng, Xinzheng Zhang. The reduction of FIB damage on cryo-lamella by lowering energy of ion beam revealed by a quantitative analysis. Structure[J]. 2023, [5] Zhu, Dongjie, Shi, Huigang, Wu, Chunling, Zhang, Xinzheng. An electron counting algorithm improves imaging of proteins with low-acceleration-voltage cryo-electron microscope. COMMUNICATIONS BIOLOGY[J]. 2022, 5(1): http://dx.doi.org/10.1038/s42003-022-03284-1.
[6] su xiaodong, cao duanfang, pan xiaowei, Shi, Lifang, Liu, Zhenfeng, Dall’Osto Luca, bassi roberto, Zhang, Xinzheng, 李梅. Supramolecular assembly of chloroplast NADH dehydrogenase-like complex with photosystem I from Arabidopsis thaliana. Molecular Plant[J]. 2022, [7] Ma, Bingting, Huang, Cuiqing, Ma, Jun, Xiang, Ye, Zhang, Xinzheng. Structure of Venezuelan equine encephalitis virus with its receptor LDLRAD3. NATURE[J]. 2021, 598(7882): 677-+, http://dx.doi.org/10.1038/s41586-021-03909-1.
[8] Ma, Jun, Zhang, Shuangyue, Zhang, Xinzheng. Structure of Machupo virus polymerase in complex with matrix protein Z. NATURE COMMUNICATIONS[J]. 2021, 12(1): http://dx.doi.org/10.1038/s41467-021-26432-3.
[9] Zhou, Changping, Shi, Huigang, Zhang, Manfeng, Zhou, Lijun, Xiao, Le, Feng, Shasha, Im, Wonpil, Zhou, Min, Zhang, Xinzheng, Huang, Yihua. Structural Insight into Phospholipid Transport by the MlaFEBD Complex from P. aeruginosa. JOURNAL OF MOLECULAR BIOLOGYnull. 2021, 433(13): http://dx.doi.org/10.1016/j.jmb.2021.166986.
[10] Wu, Chunling, Shi, Huigang, Zhu, Dongjie, Fan, Kelong, Zhang, Xinzheng. Low-cooling-rate freezing in biomolecular cryo-electron microscopy for recovery of initial frames. QRB Discovery[J]. 2021, [11] Li, Bufan, Zhu, Dongjie, Shi, Huigang, Zhang, Xinzheng. Effect of charge on protein preferred orientation at the air-water interface in cryo-electron microscopy. JOURNAL OF STRUCTURAL BIOLOGY[J]. 2021, 213(4): http://dx.doi.org/10.1016/j.jsb.2021.107783.
[12] Cheng, Jing, Li, Bufan, Si, Long, Zhang, Xinzheng. Determining structures in a native environment using single-particle cryoelectron microscopy images. THE INNOVATION[J]. 2021, 2(4): [13] Gao, Yina, Cao, Duanfang, Zhu, Jingpeng, Feng, Han, Luo, Xiu, Liu, Songqing, Yan, XiaoXue, Zhang, Xinzheng, Gao, Pu. Structural insights into assembly, operation and inhibition of a type I restriction-modification system. NATURE MICROBIOLOGY[J]. 2020, 5(9): 1107-+, https://search.proquest.com/docview/2408842246.
[14] Zhang, Manfeng, Shi, Huigang, Zhang, Xuemei, Zhang, Xinzheng, Huang, Yihua. Cryo-EM structure of the nonameric CsgG-CsgF complex and its implications for controlling curli biogenesis in Enterobacteriaceae. PLOS BIOLOGY[J]. 2020, 18(6): https://doaj.org/article/2be8ddc02a784c3493b9bea5f4770f0d.
[15] Wang, Nan, Chen, Wenyuan, Zhu, Ling, Zhu, Dongjie, Feng, Rui, Wang, Jialing, Zhu, Bin, Zhang, Xinzheng, Chen, Xiaoqing, Liu, Xianjie, Yan, Runbin, Ni, Dongyao, Zhou, Grace Guoying, Liu, Hongrong, Rao, Zihe, Wang, Xiangxi. Structures of the portal vertex reveal essential protein-protein interactions for Herpesvirus assembly and maturation. PROTEIN & CELL[J]. 2020, 11(5): 366-373, http://lib.cqvip.com/Qikan/Article/Detail?id=7102116302.
[16] Cao, Peng, Cao, Duanfang, Si, Long, Su, Xiaodong, Tian, Lijin, Chang, Wenrui, Liu, Zhenfeng, Zhang, Xinzheng, Li, Mei. Structural basis for energy and electron transfer of the photosystem I-IsiA-flavodoxin supercomplex. NATURE PLANTS[J]. 2020, 6(2): 167-+, http://dx.doi.org/10.1038/s41477-020-0593-7.
[17] Song, Guangtao, Xu, Benjin, Shi, Huigang, Zhang, Yong, Zhang, Dejiu, Cao, Xintao, Liu, Zengrui, Guo, Ran, Guan, YanZhong, Chu, Yanhui, Zhang, Xinzheng, Lou, Jizhong, Qin, Yan. Conformational activation of ribosome recycling by intra- and inter-molecular dynamics of RRF. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES[J]. 2020, 160: 1212-1219, http://dx.doi.org/10.1016/j.ijbiomac.2020.05.254.
[18] Xiaowei Pan, Duanfang Cao, Fen Xie, Fang Xu, Xiaodong Su, Hualing Mi, Xinzheng Zhang, Mei Li. Structural basis for electron transport mechanism of complex I-like photosynthetic NAD(P)H dehydrogenase. NATURE COMMUNICATIONS[J]. 2020, 11(1): 1-11, https://doaj.org/article/180426432beb4dab9a0b62320c81459e.
[19] Fan, Xiaoyi, Cao, Duanfang, Kong, Lingfei, Zhang, Xinzheng. Cryo-EM analysis of the post-fusion structure of the SARS-CoV spike glycoprotein. NATURE COMMUNICATIONS[J]. 2020, 11(1): 1-10, http://dx.doi.org/10.1038/s41467-020-17371-6.
[20] Xu, Xin, Shi, Huigang, Gong, Xiaowen, Chen, Pu, Gao, Ying, Zhang, Xinzheng, Xiang, Song. Structural insights into sodium transport by the oxaloacetate decarboxylase sodium pump. ELIFE[J]. 2020, 9: https://doaj.org/article/10777bf77a7e4996a1f05122f3150894.
[21] Cao, Duanfang, Han, Xiaonan, Fan, Xiaoyi, Xu, RuiMing, Zhang, Xinzheng. Structural basis for nucleosome-mediated inhibition of cGAS activity. CELL RESEARCH[J]. 2020, 30(12): 1088-1097, http://lib.cqvip.com/Qikan/Article/Detail?id=7104154142.
[22] You, Lilan, Ma, Jun, Wang, Jiuyu, Artamonova, Daria, Wang, Min, Liu, Liang, Xiang, Hua, Severinov, Konstantin, Zhang, Xinzheng, Wang, Yanli. Structure Studies of the CRISPR-Csm Complex Reveal Mechanism of Co-transcriptional Interference. CELL[J]. 2019, 176(1-2): 239-+, http://dx.doi.org/10.1016/j.cell.2018.10.052.
[23] Peng, Qi, Liu, Yuqian, Peng, Ruchao, Wang, Min, Yang, Wei, Song, Hao, Chen, Yuhai, Liu, Sheng, Han, Min, Zhang, Xinzheng, Wang, Peiyi, Yan, Jinghua, Zhang, Buchang, Qi, Jianxun, Deng, Tao, Gao, George F, Shi, Yi. Structural insight into RNA synthesis by influenza D polymerase. NATURE MICROBIOLOGY[J]. 2019, 4(10): 1750-1759, https://www.webofscience.com/wos/woscc/full-record/WOS:000487286800020.
[24] Liu, Sheng, Luo, Yuzi, Wang, Yajuan, Li, Shihua, Zhao, Zhennan, Bi, Yuhai, Sun, Junqing, Peng, Ruchao, Song, Hao, Zhu, Dongjie, Sun, Yuan, Li, Su, Zhang, Li, Wang, Wei, Sun, Yeping, Qi, Jianxun, Yan, Jinghua, Shi, Yi, Zhang, Xinzheng, Wang, Peiyi, Qiu, HuaJi, Gao, George F. Cryo-EM Structure of the African Swine Fever Virus. CELL HOST & MICROBE[J]. 2019, 26(6): 836-+, http://dx.doi.org/10.1016/j.chom.2019.11.004.
[25] Wu, Chunling, Huang, Xiaojun, Cheng, Jing, Zhu, Dongjie, Zhang, Xinzheng. High-quality, high-throughput cryo-electron microscopy data collection via beam tilt and astigmatism-free beam-image shift. JOURNAL OF STRUCTURAL BIOLOGY[J]. 2019, 208(3): http://dx.doi.org/10.1016/j.jsb.2019.09.013.
[26] Qianglin Fang, Dongjie Zhu, Irina Agarkova, Jagat Adhikari, Thomas Klose, Yue Liu, Zhenguo Chen, Yingyuan Sun, Michael L Gross, James L Van Etten, Xinzheng Zhang, Michael G Rossmann. Near-atomic structure of a giant virus. NATURE COMMUNICATIONS[J]. 2019, 10(1): 1-11, https://doaj.org/article/957b37d27371401a819badbfcede8eec.
[27] Su, Xiaodong, Ma, Jun, Pan, Xiaowei, Zhao, Xuelin, Chang, Wenrui, Liu, Zhenfeng, Zhang, Xinzheng, Li, Mei. Antenna arrangement and energy transfer pathways of a green algal photosystem-I-LHCI supercomplex. NATURE PLANTS[J]. 2019, 5(3): 273-281, https://www.webofscience.com/wos/woscc/full-record/WOS:000471017200011.
[28] Pan, Xiaowei, Ma, Jun, Su, Xiaodong, Cao, Peng, Chang, Wenrui, Liu, Zhenfeng, Zhang, Xinzheng, Li, Mei. Structure of the maize photosystem I supercomplex with light-harvesting complexes I and II. SCIENCE[J]. 2018, 360(6393): 1109-1112, https://www.webofscience.com/wos/woscc/full-record/WOS:000434635500043.
[29] David, Liron, Li, Yang, Ma, Jun, Garner, Ethan, Zhang, Xinzheng, Wu, Hao. Assembly mechanism of the CARMA1-BCL10-MALT1-TRAF6 signalosome. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2018, 115(7): 1499-1504, https://www.webofscience.com/wos/woscc/full-record/WOS:000424876000066.
[30] Jialing Wang, Shuai Yuan, Dongjie Zhu, Hao Tang, Nan Wang, Wenyuan Chen, Qiang Gao, Yuhua Li, Junzhi Wang, Hongrong Liu, Xinzheng Zhang, Zihe Rao, Xiangxi Wang. Structure of the herpes simplex virus type 2 C-capsid with capsid-vertex-specific component. NATURE COMMUNICATIONS[J]. 2018, 9(1): https://doaj.org/article/e0d44ce22fca47ef9b1e63e0558a8d9f.
[31] Liang, Liu, Xueyan, Li, Jun, Ma, Zongqiang, Li, Lilan, You, Jiuyu, Wang, Min, Wang, 章新政, 王艳丽. RNA介导切割RNA的Cas13a复合物分子结构. 科学新闻[J]. 2018, 45-45, http://lib.cqvip.com/Qikan/Article/Detail?id=675303202.
[32] Dongjie Zhu, Xiangxi Wang, Qianglin Fang, James L Van Etten, Michael G Rossmann, Zihe Rao, Xinzheng Zhang. Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions. NATURE COMMUNICATIONS[J]. 2018, 9(1): https://doaj.org/article/263bdefaacf84df18ada17919d4942a5.
[33] Chen, Lihong, Wang, Ming, Zhu, Dongjie, Sun, Zhenzhao, Ma, Jun, Wang, Jinglin, Kong, Lingfei, Wang, Shida, Liu, Zaisi, Wei, Lili, He, Yuwen, Wang, Jingfei, Zhang, Xinzheng. Implication for alphavirus host-cell entry and assembly indicated by a 3.5 angstrom resolution cryo-EM structure. NATURE COMMUNICATIONS[J]. 2018, 9: https://www.webofscience.com/wos/woscc/full-record/WOS:000453244600009.
[34] Mei Li, Xiaowei Pan, Jun Ma, Xiaodong Su, Wenrui Chang, Zhenfeng Liu, Xinzheng Zhang. Cryo-EM structure of maize PSI-LHCI-LHCII supercomplex. BBA - BIOENERGETICS. 2018, 1859: e34-e34, http://dx.doi.org/10.1016/j.bbabio.2018.09.109.
[35] Yuan, Shuai, Wang, Jialing, Zhu, Dongjie, Wang, Nan, Gao, Qiang, Chen, Wenyuan, Tang, Hao, Wang, Junzhi, Zhang, Xinzheng, Liu, Hongrong, Rao, Zihe, Wang, Xiangxi. Cryo-EM structure of a herpesvirus capsid at 3.1 angstrom. SCIENCE[J]. 2018, 360(6384): 48-+, https://www.webofscience.com/wos/woscc/full-record/WOS:000429263100042.
[36] Xinzheng Zhang. Structure of the Herpesvirus simplex virus type2 C-capsid with capsid-vertex-specific-component. Nature Communications. 2018, [37] Peng, Ruchao, Xu, Ying, Zhu, Tengfei, Li, Ningning, Qi, Jianxun, Chai, Yan, Wu, Min, Zhang, Xinzheng, Shi, Yi, Wang, Peiyi, Wang, Jiawei, Gao, Ning, Gao, George Fu. Alternate binding modes of anti-CRISPR viral suppressors AcrF1/2 to Csy surveillance complex revealed by cryo-EM structures. CELL RESEARCH[J]. 2017, 27(7): 853-864, http://dx.doi.org/10.1038/cr.2017.79.
[38] Yuan Yuan, Duanfang Cao, Yanfang Zhang, Jun Ma, Jianxun Qi, Qihui Wang, Guangwen Lu, Ying Wu, Jinghua Yan, Yi Shi, Xinzheng Zhang, George F Gao. Cryo-EM structures of MERS-CoV and SARS-CoV spike glycoproteins reveal the dynamic receptor binding domains. NATURE COMMUNICATIONS[J]. 2017, 8(1): https://doaj.org/article/0a48ce79c98942e39775d23cb6720f81.
[39] Su, Xiaodong, Ma, Jun, Wei, Xuepeng, Cao, Peng, Zhu, Dongjie, Chang, Wenrui, Liu, Zhenfeng, Zhang, Xinzheng, Li, Mei. Structure and assembly mechanism of plant C2S2M2-type PSII-LHCII supercomplex. SCIENCE[J]. 2017, 357(6353): 816-+, https://www.webofscience.com/wos/woscc/full-record/WOS:000408327900046.
[40] 马军, 孔令斐, 章新政. 高分辨单颗粒分析在生物样品中的应用. 物理[J]. 2017, 84-90, [41] Liu, Liang, Li, Xueyan, Ma, Jun, Li, Zongqiang, You, Lilan, Wang, Jiuyu, Wang, Min, Zhang, Xinzheng, Wang, Yanli. The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a. CELL[J]. 2017, 170(4): 714-+, http://dx.doi.org/10.1016/j.cell.2017.06.050.
[42] Luo, Qingshan, Yang, Xu, Yu, Shan, Shi, Huigang, Wang, Kun, Xiao, Le, Zhu, Guangyu, Sun, Chuanqi, Li, Tingting, Li, Dianfan, Zhang, Xinzheng, Zhou, Min, Huang, Yihua. Structural basis for lipopolysaccharide extraction by ABC transporter LptB(2)FG. NATURE STRUCTURAL & MOLECULAR BIOLOGY[J]. 2017, 24(5): 469-+, http://dx.doi.org/10.1038/nsmb.3399.
[43] Wang, Jiuyu, Ma, Jun, Cheng, Zhi, Meng, Xu, You, Lilan, Wang, Min, Zhang, Xinzheng, Wang, Yanli. A CRISPR evolutionary arms race: structural insights into viral anti-CRISPR/Cas responses. CELL RESEARCH[J]. 2016, 26(10): 1165-1168, http://lib.cqvip.com/Qikan/Article/Detail?id=670592062.
[44] Wei, Xuepeng, Su, Xiaodong, Cao, Peng, Liu, Xiuying, Chang, Wenrui, Li, Mei, Zhang, Xinzheng, Liu, Zhenfeng. Structure of spinach photosystem II-LHCII supercomplex at 3.2 angstrom resolution. NATURE[J]. 2016, 534(7605): 69-+, https://www.webofscience.com/wos/woscc/full-record/WOS:000376962300034.
[45] Zhang, Liman, Chen, Shuobing, Ruan, Jianbin, Wu, Jiayi, Tong, Alexander B, Yin, Qian, Li, Yang, David, Liron, Lu, Alvin, Wang, Wei Li, Marks, Carolyn, Ouyang, Qi, Zhang, Xinzheng, Mao, Youdong, Wu, Hao. Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. SCIENCE[J]. 2015, 350(6259): 404-409, https://www.webofscience.com/wos/woscc/full-record/WOS:000363213400032.
[46] Zhang, Xinzheng, Sun, Lei, Rossmann, Michael G. Temperature dependent conformational change of dengue virus. CURRENT OPINION IN VIROLOGY[J]. 2015, 12: 109-112, http://dx.doi.org/10.1016/j.coviro.2015.04.006.
[47] Zhang, Xinzheng, Sheng, Ju, Austin, S Kyle, Hoornweg, Tabitha E, Smit, Jolanda M, Kuhn, Richard J, Diamond, Michael S, Rossmann, Michael G. Structure of Acidic pH Dengue Virus Showing the Fusogenic Glycoprotein Trimers. JOURNAL OF VIROLOGY[J]. 2015, 89(1): 743-750, https://www.webofscience.com/wos/woscc/full-record/WOS:000347176100062.
[48] Sun, Lei, Zhang, Xinzheng, Gao, Song, Rao, Prashant A, PadillaSanchez, Victor, Chen, Zhenguo, Sun, Siyang, Xiang, Ye, Subramaniam, Sriram, Rao, Venigalla B, Rossmann, Michael G. Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution. NATURE COMMUNICATIONS[J]. 2015, 6: https://www.webofscience.com/wos/woscc/full-record/WOS:000358853600001.
[49] Sun, Lei, Young, Lindsey N, Zhang, Xinzheng, Boudko, Sergei P, Fokine, Andrei, Zbornik, Erica, Roznowski, Aaron P, Molineux, Ian J, Rossmann, Michael G, Fane, Bentley A. Icosahedral bacteriophage Phi X174 forms a tail for DNA transport during infection. NATURE[J]. 2014, 505(7483): 432-+, https://www.webofscience.com/wos/woscc/full-record/WOS:000329621800049.
[50] Xinzheng Zhang. Dengue structure differs at the temperatures of its human and mosquito host. Proc. Natl. Acad. Sci. 2013, [51] Xinzheng Zhang. Structure of Sputnik, a virophage, at 3.5-A resolution. Proc. Natl. Acad. Sci.. 2012, [52] Zhang, Xinzheng, Xiang, Ye, Dunigan, David D, Klose, Thomas, Chipman, Paul R, Van Etten, James L, Rossmann, Michael G. Three-dimensional structure and function of the Paramecium bursaria chlorella virus capsid. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2011, 108(36): 14837-14842, https://www.webofscience.com/wos/woscc/full-record/WOS:000294543400030.

   

（ 1 ） NLRP3炎症小体相关蛋白质复合物的结构及功能机制的冷冻电子显微学研究, 主持, 国家级, 2016-01--2019-12
（ 2 ） 蛋白质机器的原子分辨率冷冻电镜单颗粒分析技术及应用, 主持, 国家级, 2017-07--2022-06
（ 3 ） 算法、理论优化实现单颗粒原子分辨率结构解析, 参与, 部委级, 2017-01--2018-12

（1）Pushing theresolution limit by correcting the Ewald sphere effect in single-particleCryo-EM reconstructions   第十一届郭可信冷冻电镜国际研讨会   2018-08-28
（2）The Breakthrough of the Resolution Limit in cryo-EM SPA    2017全国电镜大会   2017-11-18
（3）The Breakthrough of the Resolution Limit in cryo-EM SPA    第五届全国冷冻电子显微学与结构生物学专题研讨会   2017-11-01

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