基因编辑

CRISPR/Cas9

疾病动物模型

   

071006-神经生物学
071008-发育生物学

Disease Models in Non-Human Pr

2009-09--2012-07   中国科学院上海生命科学研究院   博士
2007-09--2009-07   中国科学院上海生命科学研究院   硕士
2003-09--2007-07   上海交通大学   学士

博士

博士

   

2014-05~现在, 中国科学院上海生命科学研究院, 研究员
2012-12~2014-04,美国麻省理工Whitehead研究所, 博士后
2012-08~2012-12,中国科学院上海生命科学研究院, 助理研究员

神经生物学

   

（1） 中科院上海分院杰出青年科技创新人才奖, , 院级, 2018
（2） 上海市青年拔尖人才, 市地级, 2015
（3） 优秀青年科学基金, 国家级, 2015
（4） 上海市研究生优秀成果（学位论文）, 市地级, 2014
（5） 中组部青年****, 国家级, 2014
（6） 中国科学院优秀博士学位论文奖, 院级, 2013
（7） 吴瑞奖学金, 国家级, 2012
（8） 中国科学院院长奖, 特等奖, 院级, 2012
（9） 礼来奖学金, 一等奖, 其他, 2012

[1] 杨辉, 黄佳, 贺冰冰. 在细胞和成体水平检测蛋白质旁切效应的系统. CN: CN115386560A, 2022-11-25.
[2] 杨辉, 周海波, 高妮. 一种检测细胞内源性低丰度基因和lncRNA水平的方法. CN: CN114591949A, 2022-06-07.
[3] 杨辉, 周海波. Ptbp1抑制剂在预防和/或治疗功能性神经元死亡相关的神经系统疾病中的应用. CN: CN112451669A, 2021-03-09.
[4] 杨辉, 胥春龙, 周英思, 肖庆全. VI-E型和VI-F型CRISPR-Cas系统及用途. CN: CN112410377A, 2021-02-26.
[5] 杨辉, 陈万金, 左二伟. 单碱基编辑介导的剪接修复在制备治疗脊髓性肌萎缩症中的应用. CN: CN113444722A, 2021-09-28.
[6] 杨辉, 周昌阳. 基于RNA定点编辑的抑制脉络膜新生血管形成的方法及试剂. CN: CN112143701A, 2020-12-29.
[7] 杨辉, 周昌阳. 一种新型的单碱基编辑技术及其应用. CN: CN112048497A, 2020-12-08.
[8] 杨辉, 姚璇, 王兴, 施霖宇. 高效感染支持细胞的AAV突变体. CN: CN111909246A, 2020-11-10.
[9] 杨辉, 姚璇, 胡新德. 一种感染支持细胞和毛细胞的AAV载体. CN: CN110129368B, 2021-03-09.

   

[1] Cell. 2024,   通讯作者  
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[3] Li, Guoling, Jin, Ming, Li, Zhifang, Xiao, Qingquan, Lin, Jiajia, Yang, Dong, Liu, Yuanhua, Wang, Xing, Xie, Long, Ying, Wenqin, Wang, Haoqiang, Zuo, Erwei, Shi, Linyu, Wang, Ning, Chen, Wanjin, Xu, Chunlong, Yang, Hui. Mini-dCas13X-mediated RNA editing restores dystrophin expression in a humanized mouse model of Duchenne muscular dystrophy. JOURNAL OF CLINICAL INVESTIGATION[J]. 2023, 第 17 作者  通讯作者  133(3): http://dx.doi.org/10.1172/JCI162809.
[4] Jinhui Li, Zhixin Shen, Yajing Liu, Zixiang Yan, Yuanhua Liu, Xiang Lin, Junjie Tang, Ruimin Lv, Guannan Geng, ZhiQi Xiong, Changyang Zhou, Hui Yang. A high-fidelity RNA-targeting Cas13 restores paternal Ube3a expression and improves motor functions in Angelman syndrome mice. MOLECULAR THERAPY[J]. 2023, 第 12 作者  通讯作者  31(7): 2286-2295, http://dx.doi.org/10.1016/j.ymthe.2023.02.015.
[5] Wang, Xing, Zhang, Renxia, Yang, Dong, Li, Guoling, Fan, Zhanqing, Du, Hongting, Wang, Zikang, Liu, Yuanhua, Lin, Jiajia, Wu, Xiaoqing, Shi, Linyu, Yang, Hui, Zhou, Yingsi. Develop a Compact RNA Base Editor by Fusing ADAR with Engineered EcCas6e. ADVANCED SCIENCE[J]. 2023, 第 12 作者  通讯作者  10(17): https://doaj.org/article/2cb891a0e4914be98820a1a5acf40840.
[6] Guo, Ruochen, Li, Zhifang, Li, Guoling, Zhang, Hainan, Zhang, Chang, Huo, Xiaona, Zhang, Xiaoyin, Yang, Xiali, Yang, Rongrong, Liu, Yuanhua, Sun, Xiaozhi, Liu, Xinyu, Yang, Hui, Zhou, Yingsi, Xu, Chunlong. In vivo treatment of tyrosinaemia with hypercompact Cas12f1. CELL DISCOVERY. 2023, 第 13 作者  通讯作者  9(1): http://dx.doi.org/10.1038/s41421-023-00554-y.
[7] Zixiang Yan, Yuqin Yao, Luyao Li, Lingqiong Cai, Haiwei Zhang, Shenghai Zhang, Qingquan Xiao, Xing Wang, Erwei Zuo, Chunlong Xu, Jihong Wu, Hui Yang. Treatment of autosomal dominant retinitis pigmentosa caused by RHO-P23H mutation with high-fidelity Cas13X in mice. MOLECULAR THERAPY - NUCLEIC ACIDS[J]. 2023, 第 12 作者  通讯作者  33: 750-761, http://dx.doi.org/10.1016/j.omtn.2023.08.002.
[8] Yuanyuan Xue, Yong Tao, Xing Wang, Xueling Wang, Yilai Shu, Yuanhua Liu, Wen Kang, Sifan Chen, Zhenzhe Cheng, Boou Yan, Yanwei Xie, Lanting Bi, Haitao Jia, Jinhui Li, Qingquan Xiao, Liying Chen, Xuan Yao, Linyu Shi, Hui Yang, Hao Wu. RNA base editing therapy cures hearing loss induced by OTOF gene mutation. MOLECULAR THERAPY. 2023, 第 19 作者31(12): 3520-3530, http://dx.doi.org/10.1016/j.ymthe.2023.10.019.
[9] Wei, Yinghui, Zhang, Meiling, Hu, Jing, Zhou, Yingsi, Xue, Mingxing, Yin, Jianhang, Liu, Yuanhua, Feng, Hu, Zhou, Ling, Li, Zhifang, Wang, Dongshuang, Zhang, Zhiguo, Zhou, Yin, Liu, Hongbin, Yao, Ning, Zuo, Erwei, Hu, Jiazhi, Du, Yanzhi, Li, Wen, Xu, Chunlong, Yang, Hui. Human 8-cell embryos enable efficient induction of disease-preventive mutations without off-target effect by cytosine base editor. PROTEIN & CELL[J]. 2023, 第 11 作者14(6): 416-432, http://dx.doi.org/10.1093/procel/pwac043.
[10] Xiangfeng Kong, Hainan Zhang, Guoling Li, Zikang Wang, Xuqiang Kong, Lecong Wang, Mingxing Xue, Weihong Zhang, Yao Wang, Jiajia Lin, Jingxing Zhou, Xiaowen Shen, Yinghui Wei, Na Zhong, Weiya Bai, Yuan Yuan, Linyu Shi, Yingsi Zhou, Hui Yang. Engineered CRISPR-OsCas12f1 and RhCas12f1 with robust activities and expanded target range for genome editing. NATURE COMMUNICATIONS[J]. 2023, 第 19 作者14(1): 1-13, http://dx.doi.org/10.1038/s41467-023-37829-7.
[11] Han, Dingyi, Xiao, Qingquan, Wang, Yifan, Zhang, Hainan, Dong, Xue, Li, Guoling, Kong, Xiangfeng, Wang, Shihao, Song, Jinhui, Zhang, Weihong, Zhou, Jingxing, Bi, Lanting, Yuan, Yuan, Shi, Linyu, Zhong, Na, Yang, Hui, Zhou, Yingsi. Development of miniature base editors using engineered IscB nickase. NATURE METHODS[J]. 2023, 第 16 作者  通讯作者  20(7): 1029-+, http://dx.doi.org/10.1038/s41592-023-01898-9.
[12] Tong, Huawei, Liu, Nana, Wei, Yinghui, Zhou, Yingsi, Li, Yun, Wu, Danni, Jin, Ming, Cui, Shuna, Li, Hengbin, Li, Guoling, Zhou, Jingxing, Yuan, Yuan, Zhang, Hainan, Shi, Linyu, Yao, Xuan, Yang, Hui. Programmable deaminase-free base editors for G-to-Y conversion by engineered glycosylase. NATIONAL SCIENCE REVIEW[J]. 2023, 第 16 作者  通讯作者  10(8): http://dx.doi.org/10.1093/nsr/nwad143.
[13] Yang, Guixiang, Yan, Zixiang, Wu, Xiaoqing, Zhang, Meng, Xu, Chunlong, Shi, Linyu, Yang, Hui, Fang, Kailun. Ptbp1 knockdown failed to induce astrocytes to neurons in vivo. GENE THERAPY. 2023, 第 7 作者  通讯作者  
[14] Zhang, Hainan, Kong, Xiangfeng, Xue, Mingxing, Hu, Jing, Wang, Zikang, Wei, Yinghui, Wang, Haoqiang, Zhou, Jingxing, Zhang, Weihong, Xu, Mengqiu, Shen, Xiaowen, Yin, Fengcai, Ai, Zhiyuan, Huang, Guangyan, Xia, Junhui, Song, Xueqiong, Li, Hengbin, Yuan, Yuan, Li, Jinhui, Zhong, Na, Zhang, Meiling, Zhou, Yingsi, Yang, Hui. An engineered xCas12i with high activity, high specificity, and broad PAM range. PROTEIN & CELL. 2023, 第 23 作者  通讯作者  14(7): 540-545, http://dx.doi.org/10.1093/procel/pwac052.
[15] Gao, Yanxia, Fang, Kailun, Yan, Zixiang, Zhang, Haiwei, Geng, Guannan, Wu, Weiwei, Xu, Ding, Zhang, Heng, Zhong, Na, Wang, Qifang, Cai, Minqing, Zuo, Erwei, Yang, Hui. Develop an efficient and specific AAV-based labeling system for Muller glia in mice. SCIENTIFIC REPORTS[J]. 2022, 第 13 作者  通讯作者  12(1): http://dx.doi.org/10.1038/s41598-022-27013-0.
[16] Gu, Xi, Hu, Xinde, Wang, Daqi, Xu, Zhijiao, Wang, Fang, Li, Di, Li, Genglin, Yang, Hui, Li, Huawei, Zuo, Erwei, Shu, Yilai. Treatment of autosomal recessive hearing loss via in vivo CRISPR/Cas9-mediated optimized homology-directed repair in mice. CELL RESEARCH. 2022, 第 8 作者32(7): 699-702, http://lib.cqvip.com/Qikan/Article/Detail?id=7107803350.
[17] Wei, Yinghui, Xu, Chunlong, Feng, Hu, Xu, Kui, Li, Zhifang, Hu, Jing, Zhou, Ling, Wei, Yu, Zuo, Zhenrui, Zuo, Erwei, Li, Wen, Yang, Hui, Zhang, Meiling. Human cleaving embryos enable efficient mitochondrial base-editing with DdCBE. CELL DISCOVERY[J]. 2022, 第 12 作者  通讯作者  8(1): http://dx.doi.org/10.1038/s41421-021-00372-0.
[18] Tong, Huawei, Huang, Jia, Xiao, Qingquan, He, Bingbing, Dong, Xue, Liu, Yuanhua, Yang, Xiali, Han, Dingyi, Wang, Zikang, Ying, Wenqin, Zhang, Runze, Wei, Yu, Wang, Xuchen, Xu, Chunlong, Zhou, Yingsi, Li, Yanfei, Cai, Minqing, Wang, Qifang, Xue, Mingxing, Li, Guoling, Fang, Kailun, Zhang, Hainan, Yang, Hui. High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects. NATURE BIOTECHNOLOGY. 2022, 第 23 作者  通讯作者  http://dx.doi.org/10.1038/s41587-022-01419-7.
[19] Xiao, Qingquan, Xu, Zhijiao, Xue, Yuanyuan, Xu, Chunlong, Han, Lei, Liu, Yuanhua, Wang, Fang, Zhang, Runze, Han, Shuang, Wang, Xing, Li, GengLin, Li, Huawei, Yang, Hui, Shu, Yilai. Rescue of autosomal dominant hearing loss by in vivo delivery of mini dCas13X-derived RNA base editor. SCIENCE TRANSLATIONAL MEDICINE[J]. 2022, 第 13 作者  通讯作者  14(654): 
[20] Wei, Yinghui, Li, Zhifang, Xu, Kui, Feng, Hu, Xie, Long, Li, Di, Zuo, Zhenrui, Zhang, Meiling, Xu, Chunlong, Yang, Hui, Zuo, Erwei. Mitochondrial base editor DdCBE causes substantial DNA off-target editing in nuclear genome of embryos. CELL DISCOVERY[J]. 2022, 第 10 作者  通讯作者  8(1): http://dx.doi.org/10.1038/s41421-022-00391-5.
[21] Yin, Jianhang, Fang, Kailun, Gao, Yanxia, Ou, Liqiong, Yuan, Shaopeng, Xin, Changchang, Wu, Weiwei, Wu, Weiwei, Hong, Jiaxu, Yang, Hui, Hu, Jiazhi. Safeguarding genome integrity during gene-editing therapy in a mouse model of age-related macular degeneration. NATURE COMMUNICATIONS[J]. 2022, 第 10 作者  通讯作者  13(1): http://dx.doi.org/10.1038/s41467-022-35640-4.
[22] Xue, Yuanyuan, Hu, Xinde, Wang, Daqi, Li, Di, Li, Yige, Wang, Fang, Huang, Mingqian, Gu, Xi, Xu, Zhijiao, Zhou, Jinan, Wang, Jinghan, Chai, Renjie, Shen, Jun, Chen, ZhengYi, Li, GengLin, Yang, Hui, Li, Huawei, Zuo, Erwei, Shu, Yilai. Gene editing in a Myo6 semi-dominant mouse model rescues auditory function. MOLECULAR THERAPY[J]. 2022, 第 16 作者30(1): 105-118, http://dx.doi.org/10.1016/j.ymthe.2021.06.015.
[23] Xu, Chunlong, Zhou, Yingsi, Xiao, Qingquan, He, Bingbing, Geng, Guannan, Wang, Zikang, Cao, Birong, Dong, Xue, Bai, Weiya, Wang, Yifan, Wang, Xiang, Zhou, Dongming, Yuan, Tanglong, Huo, Xiaona, Lai, Jinsheng, Yang, Hui. Programmable RNA editing with compact CRISPR-Cas13 systems from uncultivated microbes. NATURE METHODS[J]. 2021, 第 16 作者  通讯作者  18(5): 499-+, http://dx.doi.org/10.1038/s41592-021-01124-4.
[24] Gao, Ni, Hu, Jing, He, Bingbing, Ji, Zhengbang, Hu, Xinde, Huang, Jia, Wei, Yu, Peng, Jianpeng, Wei, Yinghui, Zhou, Yingsi, Shen, Xiaowen, Li, He, Feng, Xue, Xiao, Qingquan, Shi, Linyu, Sun, Yidi, Zhou, Changyang, Zhou, Haibo, Yang, Hui. Endogenous promoter-driven sgRNA for monitoring the expression of low-abundance transcripts and lncRNAs. NATURE CELL BIOLOGY[J]. 2021, 第 19 作者  通讯作者  23(1): https://www.webofscience.com/wos/woscc/full-record/WOS:000604838700001.
[25] Li, Dianrong, Chen, Jie, Guo, Jia, Li, Lin, Cai, Gaihong, Chen, She, Huang, Jia, Yang, Hui, Zhuang, Yinhua, Wang, Fengchao, Wang, Xiaodong. A phosphorylation of RIPK3 kinase initiates an intracellular apoptotic pathway that promotes prostaglandin(2 alpha)-induced corpus luteum regression. ELIFE[J]. 2021, 第 8 作者10: http://dx.doi.org/10.7554/eLife.67409.
[26] Fang, Kailun, Li, Qidan, Wei, Yu, Zhou, Changyang, Guo, Wenhui, Shen, Jiaqi, Wu, Ruoxi, Ying, Wenqin, Yu, Lu, Zi, Jin, Zhang, Yuxing, Yang, Hui, Liu, Siqi, Chen, Charlie Degui. Prediction and Validation of Mouse Meiosis-Essential Genes Based on Spermatogenesis Proteome Dynamics. MOLECULAR & CELLULAR PROTEOMICS[J]. 2021, 第 12 作者  通讯作者  20: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7950215/.
[27] Wei, Yu, Zhou, Yingsi, Liu, Yajing, Ying, Wenqin, Lv, Ruiming, Zhao, Qimeng, Zhou, Haibo, Zuo, Erwei, Sun, Yidi, Yang, Hui, Zhou, Changyang. Indiscriminate ssDNA cleavage activity of CRISPR-Cas12a induces no detectable off-target effects in mouse embryos. PROTEIN & CELL. 2021, 第 10 作者  通讯作者  12(9): 741-745, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=7064607&detailType=1.
[28] Yang, Hui, Wang, Haoyi, Jaenisch, Rudolf. Response to "Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation". GENOME BIOLOGY[J]. 2021, 第 1 作者  通讯作者  22(1): https://doaj.org/article/fea182a52670449e8656c67216f95c78.
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[30] Yajing Liu, Changyang Zhou, Shisheng Huang, Lu Dang, Yu Wei, Jun He, Yingsi Zhou, Shaoshuai Mao, Wanyu Tao, Yu Zhang, Hui Yang, Xingxu Huang, Tian Chi. A Cas-embedding strategy for minimizing off-target effects of DNA base editors. NATURE COMMUNICATIONS[J]. 2020, 第 11 作者  通讯作者  11(1): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695861/.
[31] Zuo, Erwei, Sun, Yidi, Yuan, Tanglong, He, Bingbing, Zhou, Changyang, Ying, Wenqin, Liu, Jing, Wei, Wu, Zeng, Rong, Li, Yixue, Yang, Hui. A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects. NATURE METHODS[J]. 2020, 第 11 作者  通讯作者  17(6): 600-+, http://dx.doi.org/10.1038/s41592-020-0832-x.
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[37] 谢龙, 左二伟, 杨辉. 双剑合璧——双碱基基因编辑工具的开发与应用. 生命的化学[J]. 2020, 第 3 作者40(6): 981-983, http://lib.cqvip.com/Qikan/Article/Detail?id=7102533727.
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（ 1 ） 食蟹猴孤雄单倍体干细胞及其在基因修饰猴研究中的应用, 主持, 部委级, 2016-01--2016-12
（ 2 ） 食蟹猴孤雄单倍体干细胞及其在基因修饰猴研究中的应用, 主持, 部委级, 2015-01--2015-12
（ 3 ） 利用核酸酶靶向修饰，针对镰刀型贫血、地 中海贫血和艾滋病的新型治疗方法研究, 参与, 国家级, 2015-04--2017-05

现指导学生

姚璇  01  19179  

周昌阳  02  19179  

黄子健  02  19179  

唐骋  01  19179  

黄佳  02  19179  

高妮   02  19179  

贺冰冰  01  19179  

李贺  01  19179  

王兴  01  19179