080300-光学工程
080401-精密仪器及机械
071011-生物物理学

科研及商业荧光光谱分析仪
整合光学成像、光谱分析和原子力显微镜的单分子研究平台
肺泡II型细胞的细胞器分子组装机制

1993-09--2016-08   堪萨斯大学   博士
1991-09--1993-08   堪萨斯大学   学士
1988-09--1991-07   中山大学   本科学习

   

2015-10~现在, 中国科学院大学, 岗位教授
2014-12~现在, 中国科学院生物物理研究所, 研究员
2009-04~2013-11,美国宾夕法尼亚大学医学院环境医学研究所肺成像和形态学中心, 主任
2009-04~2013-11,美国宾夕法尼亚大学医学院环境医学研究所, 研究员
2002-01~2007-03,马萨诸塞州立大学医学院, 研究助理教授
1998-09~2001-12,康乃尔大学, 博士后

细胞成像技术

   

（1） 中国科学院杰出引进技术人才, 院级, 2014

[1] 黄韶辉. 一种基于结构光照明灯相位显微镜成像装置和方法. ZL 2019 1 0381679.6, 2019-05-08.

   

[1] Lin Hua, Fei Gao, Xiaoluan Xia, Qiwei Guo, Yonghua Zhao, Shaohui Huang, Zhen Yuan. Individual-specific functional connectivity improves prediction of Alzheimer’s disease’s symptoms in elderly people regardless of APOE ε4 genotype. COMMUNICATIONS BIOLOGY. 2023, 6: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10232409/.
[2] Wang, Yanyan, Zhang, Senxin, Yang, Xinrui, Hwang, Joyce K, Zhan, Chuanzong, Lian, Chaoyang, Wang, Chong, Gui, Tuantuan, Wang, Binbin, Xie, Xia, Dai, Pengfei, Zhang, Lu, Tian, Ying, Zhang, Huizhi, Han, Chong, Cai, Yanni, Hao, Qian, Ye, Xiaofei, Liu, Xiaojing, Liu, Jiaquan, Cao, Zhiwei, Huang, Shaohui, Song, Jie, PanHammarstrom, Qiang, Zhao, Yaofeng, Alt, Frederick W, Zheng, Xiaoqi, Da, LinTai, Yeap, LengSiew, Meng, FeiLong. Mesoscale DNA feature in antibody-coding sequence facilitates somatic hypermutation. CELL[J]. 2023, 186(10): 2193-+, http://dx.doi.org/10.1016/j.cell.2023.03.030.
[3] Xia, Xiaoluan, Zeng, Xinglin, Gao, Fei, Hua, Lin, Huang, Shaohui, Yuan, Zhen. Striatonigrostriatal connectivity-based cross-species parcellation of human and macaque substantia nigra. HUMAN BRAIN MAPPING[J]. 2023, 44(12): 4590-4604, http://dx.doi.org/10.1002/hbm.26402.
[4] Wang, Dan, Wu, Shaowen, Wang, Dongdong, Song, Xingyu, Yang, Maohua, Zhang, Wolun, Huang, Shaohui, Weng, Jingwei, Liu, Zhijun, Wang, Wenning. The importance of the compact disordered state in the fuzzy interactions between intrinsically disordered proteins. CHEMICAL SCIENCE[J]. 2022, 13(8): 2363-2377, http://dx.doi.org/10.1039/d1sc06825c.
[5] Zhulou Wang, Huizhi Zhang, Lin Jian, Bo Ding, Keying Huang, Wolun Zhang, Qian Xiao, Shaohui Huang. Principles of fluorescence correlation spectroscopy applied to studies of biomolecular liquid–liquid phase separation. 生物物理学报:英文版[J]. 2022, 8(2): 100-118, http://lib.cqvip.com/Qikan/Article/Detail?id=7107209204.
[6] Li, Wenqian, Ji, Qingshan, Wei, Zhongjie, Chen, Yulei, Zhang, Zhiyong, Yin, Xueying, Aghmiuni, Samaneh Khodi, Liu, Muziying, Chen, Weirong, Shi, Lei, Chen, Quan, Du, Xinzheng, Yu, Li, Cao, MinJie, Wang, Zhulou, Huang, Shaohui, Jin, Tengchuan, Wang, Qiwei. Biochemical characterization of G64W mutant of acidic beta-crystallin 4. EXPERIMENTAL EYE RESEARCH[J]. 2019, 186: 107712-, http://dx.doi.org/10.1016/j.exer.2019.107712.
[7] Sun, Yanan, Liang, Yanqin, Dai, Wenbing, He, Bing, Zhang, Hua, Wang, Xueqing, Wang, Jiancheng, Huang, Shaohui, Zhang, Qiang. Peptide-Drug Conjugate-Based Nanocombination Actualizes Breast Cancer Treatment by Maytansinoid and Photothermia with the Assistance of Fluorescent and Photoacoustic Images. NANO LETTERS[J]. 2019, 19(5): 3229-3237, https://www.webofscience.com/wos/woscc/full-record/WOS:000467781900060.
[8] 王柱楼, 康宁, 梁艳琴, 肖茜, 黄韶辉. CorTector^TMSX100：一款桌面式荧光相关光谱仪的原理和应用. 生物化学与生物物理进展[J]. 2018, 45(11): 1166-1177, http://lib.cqvip.com/Qikan/Article/Detail?id=676786963.
[9] Xu, Lingyan, Ren, Zhiyun, Chow, Frances E, Tsai, Richard, Liu, Tongzheng, Rizzolio, Flavio, Boffo, Silvia, Xu, Yungen, Huang, Shaohui, Lippa, Carol F, Gong, Yuesong. Pathological Role of Peptidyl-Prolyl Isomerase Pin1 in the Disruption of Synaptic Plasticity in Alzheimer's Disease. NEURAL PLASTICITY[J]. 2017, 2017: https://doaj.org/article/85cfa96468324a849d4d5daf78bdd1ae.
[10] Roszell, Blair R, Tao, JianQin, Yu, Kevin J, Gao, Ling, Huang, Shaohui, Ning, Yue, Feinstein, Sheldon I, Vite, Charles H, Bates, Sandra R. Pulmonary Abnormalities in Animal Models Due to Niemann-Pick Type C1 (NPC1) or C2 (NPC2) Disease. PLOS ONE[J]. 2013, 8(7): https://www.webofscience.com/wos/woscc/full-record/WOS:000321341000031.
[11] Liao, YiJen, Lin, MengWei, Yen, ChiaHung, Lin, YuTing, Wang, ChungKwe, Huang, ShiuFeng, Chen, KuanHsuan, Yang, ChingPing, Chen, TzuLang, Hou, MingFeng, Chen, YiMing Arthur. Characterization of Niemann-Pick Type C2 Protein Expression in Multiple Cancers Using a Novel NPC2 Monoclonal Antibody. PLOS ONE[J]. 2013, 8(10): http://dx.doi.org/10.1371/journal.pone.0077586.
[12] Rahaman, Hamidur, Zhou, Suiping, Dodia, Chandra, Feinstein, Sheldon I, Huang, Shaohui, Speicher, David, Fisher, Aron B. Increased Phospholipase A(2) Activity with Phosphorylation of Peroxiredoxin 6 Requires a Conformational Change in the Protein. BIOCHEMISTRY[J]. 2012, 51(27): 5521-5530, https://www.webofscience.com/wos/woscc/full-record/WOS:000306203100015.
[13] Yu, Miao, Huang, Shaohui, Yu, Kevin Jun, Clyne, Alisa Morss. Dextran and Polymer Polyethylene Glycol (PEG) Coating Reduce Both 5 and 30 nm Iron Oxide Nanoparticle Cytotoxicity in 2D and 3D Cell Culture. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES[J]. 2012, 13(5): 5554-5570, https://doaj.org/article/0272347fd831448ea904e457d235b894.
[14] Thom, Stephen R, Yang, Ming, Bhopale, Veena M, Huang, Shaohui, Milovanova, Tatyana N. Microparticles initiate decompression-induced neutrophil activation and subsequent vascular injuries. JOURNAL OF APPLIED PHYSIOLOGY[J]. 2011, 110(2): 340-351, https://www.webofscience.com/wos/woscc/full-record/WOS:000288207100008.
[15] Shuvaev, Vladimir V, Han, Jingyan, Yu, Kevin J, Huang, Shaohui, Hawkins, Brian J, Madesh, Muniswamy, Nakada, Marian, Muzykantov, Vladimir R. PECAM-targeted delivery of SOD inhibits endothelial inflammatory response. FASEB JOURNAL[J]. 2011, 25(1): 348-357, https://www.webofscience.com/wos/woscc/full-record/WOS:000285869500031.
[16] Zhang, Linghui, Yu, Kevin, Robert, Kyle W, DeBolt, Kristine M, Hong, Nankang, Tao, JianQin, Fukuda, Mitsunori, Fisher, Aron B, Huang, Shaohui. Rab38 targets to lamellar bodies and normalizes their sizes in lung alveolar type II epithelial cells. AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY[J]. 2011, 301(4): L461-L477, https://www.webofscience.com/wos/woscc/full-record/WOS:000295357300007.
[17] Thom, Stephen R, Bhopale, Veena M, Yang, Ming, Bogush, Marina, Huang, Shaohui, Milovanova, Tatyana N. Neutrophil beta(2) Integrin Inhibition by Enhanced Interactions of Vasodilator-stimulated Phosphoprotein with S-Nitrosylated Actin. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2011, 286(37): 32854-32865, https://www.webofscience.com/wos/woscc/full-record/WOS:000294726800092.
[18] DiNitto, Jonathan P, Delprato, Anna, Lee, MengTse Gabe, Cronin, Thomas C, Huang, Shaohui, Guilherme, Adilson, Czech, Michael P, Lambright, David G. Structural basis and mechanism of autoregulation in 3-phosphoinositide-dependent Grp1 family Arf GTPase exchange factors. MOLECULAR CELL[J]. 2007, 28(4): 569-583, http://dx.doi.org/10.1016/j.molcel.2007.09.017.
[19] Huang, Shaohui, Czech, Michael P. The GLUT4 glucose transporter. CELL METABOLISMnull. 2007, 5(4): 237-252, http://dx.doi.org/10.1016/j.cmet.2007.03.006.
[20] Huang, Shaohui, Lifshitz, Larry M, Jones, Christine, Bellve, Karl D, Standley, Clive, Fonseca, Sonya, Corvera, Silvia, Fogarty, Kevin E, Czech, Michael P. Insulin stimulates membrane fusion and GLUT4 accumulation in clathrin coats on adipocyte plasma membranes. MOLECULAR AND CELLULAR BIOLOGY[J]. 2007, 27(9): 3456-3469, http://dx.doi.org/10.1128/MCB.01719-06.
[21] Saito, Tsugumichi, Jones, Christine C, Huang, Shaohui, Czech, Michael P, Pilch, Paul F. The interaction of akt with APPL1 is required for insulin-stimulated glut4 translocation. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2007, 282(44): 32280-32287, http://dx.doi.org/10.1074/jbc.M704150200.
[22] Bose, A, Guilherme, A, Huang, SH, Hubbard, AC, Lane, CR, Soriano, NA, Czech, MP. The v-SNARE Vti1a regulates insulin-stimulated glucose transport and Acrp30 secretion in 3T3-L1 adipocytes. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2005, 280(44): 36946-36951, http://dx.doi.org/10.1074/jbc.M508317200.
[23] Huang, SH, Lifshitz, L, PatkiKamath, V, Tuft, R, Fogarty, K, Czech, MP. Phosphatidylinositol-4,5-bisphosphate-rich plasma membrane patches organize active zones of endocytosis and ruffling in cultured adipocytes. MOLECULAR AND CELLULAR BIOLOGY[J]. 2004, 24(20): 9102-9123, https://www.webofscience.com/wos/woscc/full-record/WOS:000224239100028.
[24] Hess, ST, Huang, SH, Heikal, AA, Webb, WW. Biological and chemical applications of fluorescence correlation spectroscopy: A review. BIOCHEMISTRYnull. 2002, 41(3): 697-705, https://www.webofscience.com/wos/woscc/full-record/WOS:000173374500001.
[25] Huang, SH, Heikal, AA, Webb, WW. Two-photon fluorescence spectroscopy and microscopy of NAD(P)H and flavoprotein. BIOPHYSICAL JOURNAL[J]. 2002, 82(5): 2811-2825, [26] Negash, S, Huang, SH, Squier, TC. Rearrangement of domain elements of the Ca-ATPase in cardiac sarcoplasmic reticulum membranes upon phospholamban phosphorylation. BIOCHEMISTRY[J]. 1999, 38(25): 8150-8158, https://www.webofscience.com/wos/woscc/full-record/WOS:000081138400031.
[27] Huang, SH, Negash, S, Squier, TC. Erythrosin isothiocyanate selectively labels lysine(464) within an ATP-protectable binding site on the Ca-ATPase in skeletal sarcoplasmic reticulum membranes. BIOCHEMISTRY[J]. 1998, 37(19): 6949-6957, https://www.webofscience.com/wos/woscc/full-record/WOS:000073797300037.
[28] Huang, SH, Squier, TC. Enhanced rotational dynamics of the phosphorylation domain of the Ca-ATPase upon calcium activation. BIOCHEMISTRY[J]. 1998, 37(51): 18064-18073, https://www.webofscience.com/wos/woscc/full-record/WOS:000077848400048.

   

（ 1 ） Rab38在肺泡II型细胞中对板层小体生物生成的调控机制研究, 主持, 国家级, 2016-05--2018-12
（ 2 ） 中国科学院技术支撑人才计划, 主持, 部委级, 2015-01--2018-12
（ 3 ） 便携式荧光相关光谱仪：一种新型超灵敏医疗检测仪的研制, 主持, 省级, 2015-02--2017-01
（ 4 ） 应用于纳米光学单分子研究的全参数荧光检测光谱仪-原子力显微镜整合系统, 主持, 研究所（学校）, 2015-06--2017-05
（ 5 ） 生物大分子复合体研究的新技术新方法研究团队, 主持, 部委级, 2016-01--2020-12

（1）荧光相关光谱和其他单分子荧光技术的仪器研制   单分子学术研讨会   2016-05-27
（2）单分子荧光光谱和荧光成像技术   自然科学基金委双清论坛   2015-11-25
（3）Single-molecule Spectroscopy and Microscopy Based on Multiparameter Fluorescence Detection and Nanophotonic Devices   表面层析与光学显微技术国际论坛   2015-07-24

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