基本信息
王磊 男 中国科学院青岛生物能源与过程研究所
电子邮件: wanglei1982@qibebt.ac.cn
通信地址: 山东省青岛市崂山区松岭路189号
邮政编码:
电子邮件: wanglei1982@qibebt.ac.cn
通信地址: 山东省青岛市崂山区松岭路189号
邮政编码:
教育背景
2009-09--2014-07 中国科学院大连化学物理研究所生物化工专业 博士2005-09--2008-06 山东大学微生物技术国家重点实验室微生物学 硕士2001-09--2005-06 山东大学生命科学学院生物技术专业 学士
工作经历
工作简历
2022-08~现在, 中国科学院青岛生物能源与过程研究所, 副研究员2018-09~2022-07,东北电力大学化学工程学院, 专任教师,副教授2016-08~2018-08,东北电力大学化学工程学院, 专任教师,副研究员2014-06~2016-07,中国科学院大连化学物理研究所, 博士后
专利与奖励
奖励信息
(1) 辽宁省自然科学学术成果奖, 二等奖, 省级, 2023
专利成果
( 1 ) 一种编码大根香叶烯A合成酶的基因的应用, 发明专利, 2023, 第 4 作者, 专利号: CN116121230A( 2 ) 一种基于法尼烯发酵液原位合成维生素A的方法及其应用, 发明专利, 2023, 第 2 作者, 专利号: CN116375622A( 3 ) 定向进化构建高效转化白藜芦醇生产松芪的ROMT突变体, 发明专利, 2020, 第 3 作者, 专利号: CN110804618A( 4 ) 碳量子点的复合探针及检测白色念珠菌含量的方法, 发明专利, 2019, 第 4 作者, 专利号: CN109870434A( 5 ) 利用香草醛及其类似物制备姜黄素的方法, 发明专利, 2019, 第 3 作者, 专利号: CN109182398A( 6 ) 一种提高玉米秸秆水解液培养油脂酵母产油量的方法, 发明专利, 2018, 第 6 作者, 专利号: CN107557400A( 7 ) 一种NAD类似物的还原方法, 专利授权, 2019, 第 2 作者, 专利号: CN104946706B( 8 ) 一种用针状纳米材料增加细胞透性的方法, 发明专利, 2013, 第 2 作者, 专利号: CN103160438A
出版信息
发表论文
(1) Rational modification of xanthan gum based on assistance of molecular dynamics simulation, International Journal of Biological Macromolecules, 2024, 第 6 作者 通讯作者(2) In situ fermentation of ultra-strong, microplastic-free, and biodegradable multilayer bacterial cellulose film for food packaging, ACS Applied Materials & Interfaces, 2023, 第 8 作者 通讯作者(3) Creating enzymes and self-sufficient cells for biosynthesis of the non-natural cofactor nicotinamide cytosine dinucleotide, NATURE COMMUNICATIONS, 2021, 第 8 作者(4) Engineering d-Lactate Dehydrogenase to Favor an Non-natural Cofactor Nicotinamide Cytosine Dinucleotide, CHEMBIOCHEM, 2020, 第 3 作者(5) Metabolic engineering and optimization of the fermentation medium for vitamin B-12 production in Escherichia coli, BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2020, 第 6 作者(6) Protein scaffold optimizes arrangement of constituent enzymes in indigoidine synthetic pathway to improve the pigment production, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2020, 第 1 作者(7) Microbial Lipid Production from Corn Stover by the Oleaginous Yeast Rhodosporidium toruloides Using the PreSSLP Process, ENERGIES, 2019, 第 7 作者(8) Escherichia coli Strain Designed for Characterizing in Vivo Functions of Nicotinamide Adenine Dinucleotide Analogues, ORGANIC LETTERS, 2019, 第 1 作者(9) Soybean oil refinery effluent treatment and its utilization for bacterial cellulose production by Gluconacetobacter xylinus, FOOD HYDROCOLLOIDS, 2019, 第 5 作者 通讯作者(10) Trichosporon fermentans biomass flocculation from soybean oil refinery wastewater using bioflocculant produced from Paecilomyces sp. M2-1, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2019, 第 6 作者(11) Fluorimetric Detection of Candida albicans Using Cornstalk N-Carbon Quantum Dots Modified with Amphotericin B, BIOCONJUGATE CHEMISTRY, 2019, 第 5 作者(12) Structural Insights into Phosphite Dehydrogenase Variants Favoring a Non-natural Redox Cofactor, ACS CATALYSIS, 2019, 第 3 作者(13) Visualizing Soluble Protein Mutants by Using Monomeric Red Fluorescent Protein as a Reporter for Directed Evolution, APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2018, 第 2 作者(14) Refined soybean oil wastewater treatment and its utilization for lipid production by the oleaginous yeast Trichosporon fermentans, BIOTECHNOLOGY FOR BIOFUELS, 2018, 第 6 作者(15) Synthetic Cofactor-Linked Metabolic Circuits for Selective Energy Transfer, ACS CATALYSIS, 2017, 第 1 作者(16) Engineering Escherichia coli Nicotinic Acid Mononucleotide Adenylyltransferase for Fully Active Amidated NAD Biosynthesis, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2017, 第 3 作者(17) In vivo protein allylation to capture protein methylation candidates, CHEMICAL COMMUNICATIONS, 2016, 第 6 作者(18) 苹果酸酶结合域定点突变对烟酰胺腺嘌呤二核苷酸类似物催化性能的影响, Effect of Site-Directed Mutagenesis in Coenzyme-Binding Domain of Malic Enzyme on Coenzyme Activities for Nicotinamide Adenine Dinucleotide Analogs, 应用化学, 2016, 第 4 作者(19) 正交氧化还原体系及其应用, 中国科学 生命科学, 2015, 第 1 作者(20) Four-component ��-bromo-��-phosphoalkoxylation of aromatic ��,��-unsaturated carbonyl compounds, RSC ADVANCES, 2015, 第 5 作者(21) 基于海泡石的细胞透性化, Sepiolite-mediated cell permeabilization, 生物加工过程, 2015, 第 1 作者(22) Identification of UshA as a major enzyme for NAD degradation in Escherichia coli, ENZYME AND MICROBIAL TECHNOLOGY, 2014, 第 1 作者(23) Conversion of biomass-derived oligosaccharides into lipids, BIOTECHNOLOGY FOR BIOFUELS, 2014, 第 4 作者(24) Application of abiotic redox factor for synthetic biology, 2014 cold spring harbor asia conference, 2014, 第 2 作者(25) Identification of malic enzyme mutants depending on 1,2,3-triazole moiety-containing nicotinamide adenine dinucleotide analogs, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2014, 第 4 作者(26) 重组酿酒酵母催化二氢大豆苷元生产雌马酚, 中国生物工程杂志, 2014, 第 3 作者(27) Synthesis of NAD analogs to develop bioorthogonal redox system, SCIENCE CHINA-CHEMISTRY, 2013, 第 2 作者(28) An accurate method for estimation of the intracellular aqueous volume of Escherichia coli cells, JOURNAL OF MICROBIOLOGICAL METHODS, 2013, 第 1 作者(29) Engineering NAD(+) availability for Escherichia coli whole-cell biocatalysis: a case study for dihydroxyacetone production, MICROBIAL CELL FACTORIES, 2013, 第 3 作者(30) 利用人工氧还酶体系催化L-苹果酸氧化脱羧反应, Oxidative Decarboxylation of L-Malate by Using a Synthetic Bioredox System, 催化学报, 2012, 第 2 作者(31) Creation of Bioorthogonal Redox Systems Depending on Nicotinamide Flucytosine Dinucleotide, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2011, 第 2 作者(32) Assembling of Bioorthogonal Redox Systems Depending on an NAD analog, GORDON RESEARCH CONFERENCES ON BIOORGANIC CHEMISTRY, 2011, 第 2 作者(33) Assembling of bioorthogonal redox systems, ABSTRACTS OF PAPERS PRESENTED AT THE 2011 COLD SPRING HARBOR ASIA SYMPOSIUM, 2011, 第 2 作者(34) Determining the Extremes of the Cellular NAD(H) Level by Using an Escherichia coli NAD(+)-Auxotrophic Mutant, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2011, 第 2 作者(35) Assembling of bioorthogonal redox systems, 2011 COLD SPRING HARBOR ASIA SYMPOSIUM, 2011, 第 2 作者(36) Assembling of Bioorthogonal Redox Systems Depending on an NAD Analog, BITS 2ND SYMPOSIUM ON ENZYMES AND BIOCATALYSIS-2011, 2011, 第 3 作者(37) Questing the extremes of the cellular NAD(H) level using an Escherichia coli NAD+ auxotrophic mutant, GORDON RESEARCH CONFERENCES ON BIOORGANIC CHEMISTRY, 2011, 第 2 作者(38) Determining the Extremes of the Cellular NAD(H) Level by Using an Escherichia coli NAD -Auxotrophic Mutant, APPLIED AND ENVIRONMENT MICROBIOLOGY, 2011, 第 2 作者
科研活动
科研项目
( 1 ) 基于烟梗的生物合成转化技术研究及应用, 负责人, 境内委托项目, 2024-02--2026-04( 2 ) 改造铁氧还蛋白-NADP还原酶用于构建光驱动的非天然辅酶还原体系, 负责人, 国家任务, 2018-01--2020-12