蛋白质翻译后修饰方法研究

蛋白质糖基化及其在转化医学中的应用
新型蛋白质结构活性探针反应研究

蛋白质氧化与疾病关联性研究

有机化学、应用化学、化学生物学背景

070302-分析化学
0703Z1-化学生物学

蛋白质糖基化，蛋白-蛋白相互作用及蛋白质分泌组学，化学生物学
化学蛋白质组学

2007-09--2013-07   中国科学院大连化学物理研究所   理学博士
2003-09--2007-07   天津大学   理学学士

2013.07-2018.06  中国科学院大连化学物理研究所   副研究员 

2018.07---             中国科学院大连化学物理研究所   研究员 

   

（1） ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2023, 通讯作者
（2） Protein O-GlcNAcylation impairment caused by N-acetylglucosamine phosphate mutase deficiency leads to growth variations in Arabidopsis thaliana, PLANT JOURNAL, 2023, 第 3 作者
（3） 活性导向的化学探针在氨基酸反应活性表征中的应用进展, Advances in applications of activity-based chemical probes in the characterization of amino acid reactivities, 色谱, 2023, 第 4 作者
（4） 提高N-连接完整糖肽鉴定准确性的母离子特征筛选策略, Improving Confidence for the Identification of N-Linked Intact Glycopeptides Based on the Precursor Feature, 质谱学报, 2023, 第 2 作者
（5） Melatonin decreases GSDME mediated mesothelial cell pyroptosis and prevents peritoneal fibrosis and ultrafiltration failure, SCIENCE CHINA-LIFE SCIENCES, 2023, 通讯作者
（6） Immune checkpoint therapy-elicited sialylation of IgG antibodies impairs antitumorigenic type I interferon responses in hepatocellular carcinoma, IMMUNITY, 2023, 第 4 作者
（7） Loss of RBMS1 promotes anti-tumor immunity through enabling PD-L1 checkpoint blockade in triple-negative breast cancer, CELL DEATH AND DIFFERENTIATION, 2022, 第 15 作者
（8） Sirtuin-Derived Covalent Binder for the Selective Recognition of Protein Crotonylation, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2022, 通讯作者
（9） Endo-M Mediated Chemoenzymatic Approach Enables Reversible Glycopeptide Labeling for O-GlcNAcylation Analysis, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2022, 通讯作者
（10） Chemical Depletion of Histidine-Containing Peptides Allows Identification of More Low-Abundance Methylation Sites from Proteome Samples, JOURNAL OF PROTEOME RESEARCH, 2021, 第 6 作者
（11） Automated Intact Glycopeptide Enrichment Method Facilitating Highly Reproducible Analysis of Serum Site-Specific N-Glycoproteome, ANALYTICAL CHEMISTRY, 2021, 第 5 作者
（12） 完整糖基化肽段的富集与质谱解析新技术研究进展, Recent advances in glycopeptide enrichment and mass spectrometry data interpretation approaches for glycoproteomics analyses, 色谱, 2021, 第 2 作者
（13） Highly Efficient Enrichment of O-GalNAc Glycopeptides by Using Immobilized Metal Ion Affinity Chromatography, ANALYTICAL CHEMISTRY, 2021, 通讯作者
（14） 提高开放性检索鉴定灵敏度的互补离子谱图策略, Complementary Ion Spectrum Strategy for Improving the Sensitivity of Open Search, 质谱学报, 2020, 第 4 作者
（15） Functional Nanochannels for Sensing Tyrosine Phosphorylation, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2020, 第 9 作者
（16） 羰基化蛋白质组学分析进展, Progress in Proteomic Analysis of Protein Carbonylation, 分析测试学报, 2020, 第 2 作者
（17） A Mass-Spectrometry-Based Antibody-Free Approach Enables the Quantification of D-Dimer in Plasma, JOURNAL OF PROTEOME RESEARCH, 2020, 第 3 作者
（18） Profiling of Endogenously Intact N-Linked and O-Linked Glycopeptides from Human Serum Using an Integrated Platform, JOURNAL OF PROTEOME RESEARCH, 2020, 第 2 作者
（19） Proteomics analysis reveals the defense priming effect of chitosan oligosaccharides in Arabidopsis-Pst DC3000 interaction, PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2020, 第 2 作者
（20） Multi-histidine functionalized material for the specific enrichment of sialylated glycopeptides, JOURNAL OF CHROMATOGRAPHY A, 2020, 第 2 作者
（21） Highly Efficient Separation of Methylated Peptides Utilizing Selective Complexation between Lysine and 18-Crown-6, ANALYTICAL CHEMISTRY, 2020, 第 4 作者
（22） An overview on enrichment methods for cell surface proteome profiling, JOURNAL OF SEPARATION SCIENCE, 2020, 第 2 作者
（23） Highly Efficient Analysis of Glycoprotein Sialylation in Human Serum by Simultaneous Quantification of Glycosites and Site-Specific Glycoforms, JOURNAL OF PROTEOME RESEARCH, 2019, 第 1 作者
（24） A New Searching Strategy for the Identification of O-Linked Glycopeptides, ANALYTICAL CHEMISTRY, 2019, 第 3 作者
（25） Proteomics Analysis of Site-specific Glycoforms by a Virtual Multistage Mass Spectrometry Metho, Analytica Chimica Acta, 2019, 第 1 作者
（26） Proteomics analysis of site-specific glycoforms by a virtual multistage mass spectrometry method, ANALYTICA CHIMICA ACTA, 2019, 第 1 作者
（27） Recent advances in methods for the analysis of protein o-glycosylation at proteome level, JOURNAL OF SEPARATION SCIENCE, 2018, 第 2 作者
（28） Lcn1 is highly expressed in cholangiocarcinoma patients and indicates poor prognosis, INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, 2018, 第 4 作者
（29） Highly Efficient Identification of O-GalNAc Glycosylation by an Acid-Assisted Glycoform Simplification Approach, PROTEOMICS, 2018, 第 2 作者
（30） Chemoenzymatic approach for the proteomics analysis of mucin-type core-1 o-glycosylation in human serum, ANALYTICAL CHEMISTRY, 2018, 第 4 作者
（31） Caffeic acid phenethyl ester (CAPE) revisited: Covalent modulation of XPO1/CRM1 activities and implication for its mechanism of action, CHEMICAL BIOLOGY & DRUG DESIGN, 2017, 第 3 作者
（32） In-Depth Analysis of Glycoprotein Sialylation in Serum Using a Dual-Functional Material with Superior Hydrophilicity and Switchable Surface Charge, ANALYTICAL CHEMISTRY, 2017, 第 2 作者
（33） Sensitive, Robust, and Cost-Effective Approach for Tyrosine Phosphoproteome Analysis, ANALYTICAL CHEMISTRY, 2017, 第 7 作者
（34） Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study, TALANTA, 2017, 第 6 作者
（35） Proteomics analysis of o-gainac glycosylation in human serum by an integrated strategy, ANALYTICAL CHEMISTRY, 2017, 第 1 作者
（36） Characterization of site-specific glycosylation of secreted proteins associated with multi-drug resistance of gastric cancer, ONCOTARGET, 2016, 第 2 作者
（37） Dual-Metal Centered Zirconium-Organic Framework: A Metal-Affinity Probe for Highly Specific Interaction with Phosphopeptides, ACS APPLIED MATERIALS & INTERFACES, 2016, 第 8 作者
（38） Specific Enrichment of Peptides with N-Terminal Serine/Threonine by a Solid-Phase Capture-Release Approach for Efficient Proteomics Analysis, ANALYTICAL CHEMISTRY, 2015, 第 5 作者
（39） Selective Enrichment of Cysteine-Containing Phosphopeptides for Subphosphoproteome Analysis, JOURNAL OF PROTEOME RESEARCH, 2015, 第 6 作者
（40） Highly Efficient Release of Glycopeptides from Hydrazide Beads by Hydroxylamine Assisted PNGase F Deglycosylation for N‑Glycoproteome Analysis, ANALYTICAL CHEMISTRY, 2015, 第 9 作者
（41） Amine Chemistry Method for Selective Enrichment of N‑Linked Glycopeptides for Glycoproteomics Analysis, JOURNAL OF PROTEOME RESEARCH, 2015, 第 6 作者
（42） AN INTEGRATED METHOD FOR DETERMINATION OF SITE-SPECIFIC GLYCOFORMS IN EPITHELIAL-MESENCHYMAL TRANSITION (EMT) PROCESS, 43rd international symposium on high performance liquid phase separations and related techniques, 2015, 第 2 作者
（43） Development of Methods for Site-Specifi Analysis of N-Linked Protein Glycosylation, hupo 2015 world congress, 2015, 第 1 作者
（44） Imine—linked conjugated organic polymer bearing bis(imino) pyridine ligands and its catalytic application in C–C coupling reactions, CHINESE JOURNAL OF CATALYSIS催化学报, 2015, 第 5 作者
（45） Highly Efficient Release of Glycopeptides from Hydrazide Beads by Hydroxylamine Assisted PNGase F Deglycosylation for N-Glycoproteome Analysis, ANALYTICAL CHEMISTRY, 2015, 第 9 作者
（46） AN AMINE CHEMISTRY METHOD FOR SELECTIVE ENRICHMENT OF N-LINKED GLYCOPEPTIDES FOR GLYCOPROTEOMICS ANALYSIS, 31st international symposium on microscale bioseparation, 2015, 第 4 作者
（47） Specific Enrichment of Peptides with N‑Terminal Serine/Threonine by a Solid-Phase Capture-Release Approach for Efficient Proteomics Analysis, ANALYTICAL CHEMISTRY, 2015, 第 5 作者
（48） Amine Chemistry Method for Selective Enrichment of N-Linked Glycopeptides for Glycoproteomics Analysis, JOURNAL OF PROTEOME RESEARCH, 2015, 第 6 作者
（49） Interference in Autophagosome Fusion by Rare Earth Nanoparticles Disrupts Autophagic Flux and Regulation of an Interleukin-1β Producing Inflammasome, ACS NANO, 2014, 第 3 作者
（50） Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides, ANALYST, 2014, 第 3 作者
（51） Interference in Autophagosome Fusion by Rare Earth Nanoparticles Disrupts Autophagic Flux and Regulation of an Interleukin-1 beta Producing Inflammasome, ACS NANO, 2014, 第 3 作者
（52） In Situ Sample Processing Approach (iSPA) for Comprehensive Quantitative Phosphoproteome Analysis, JOURNAL OF PROTEOME RESEARCH, 2014, 第 2 作者
（53） 原位富集-选择性标记技术用于蛋白质翻译后修饰的定量分析, The 5th Asia Oceania Mass Spectrometry Conference & The 33rd Chinese Mass Spectrometry Society Annual Conference, 2014, 第 1 作者
（54） Highly efficient N-glycoproteomic sample preparation by combining C-18 and graphitized carbon adsorbents, ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2014, 第 7 作者
（55） Identification of phosphopeptides with unknown cleavage specificity by a de novo sequencing assisted database search strategy, PROTEOMICS, 2014, 第 6 作者
（56） Multiplex isotope dimethyl labeling of substrate peptides for high throughput kinase activity assay via quantitative MALDI MS, CHEMICAL COMMUNICATIONS, 2014, 第 7 作者
（57） Synthesis of zwitterionic polymer brushes hybrid silica nanoparticles via controlled polymerization for highly efficient enrichment of glycopeptides, ANALYTICA CHIMICA ACTA, 2014, 第 3 作者
（58） Five-plex isotope dimethyl labeling for quantitative proteomics, CHEMICAL COMMUNICATIONS, 2014, 第 4 作者
（59） In vivo detection of magnetic labeled oxidized multi-walled carbon nanotubes by magnetic resonance imaging, NANOTECHNOLOGY, 2014, 第 4 作者
（60） One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides, ANALYTICA CHIMICA ACTA, 2014, 第 3 作者
（61） Imine-linked conjugated organic polymer bearing bis(imino)pyridine ligands and its catalytic application in C-C coupling reactions, CHINESE JOURNAL OF CATALYSIS, 2014, 第 5 作者
（62） 一种嵌有双亚胺基吡啶配体的全共轭有机聚合物材料及其在C-C偶联反应中的应用, Imine-linked conjugated organic polymer bearing bis(imino)pyridine ligands and its catalytic application in C-C coupling reactions, 催化学报, 2014, 第 5 作者
（63） 一种嵌有双亚胺基吡啶配体的全共轭有机聚合物材料及其在C-C偶联反应中的应用, Imine-linked conjugated organic polymer bearing bis(imino)pyridine ligands and its catalytic application in C-C coupling reactions, 催化学报, 2014, 第 5 作者
（64） Trypsin-Catalyzed N-Terminal Labeling of Peptides with Stable Isotope-Coded Affinity Tags for Proteome Analysis, ANALYTICALCHEMISTRY, 2014, 第 9 作者
（65） Solid-phase based technologies and methods for glycoproteome and phsophoproteome analysis, 39TH INTERNATIONAL SYMPOSIUM ON HIGH PERFORMANCE LIQUID PHASE SEPARATIONS, 2013, 第 6 作者
（66） Highly Efficient Extraction of the Cellular Nucleic Acid Associated Protein in Vitro with Magnetic Oxidized Carbon Nanotube, 39TH INTERNATIONAL SYMPOSIUM ON HIGH PERFORMANCE LIQUID PHASE SEPARATIONS, 2013, 第 3 作者
（67） Polyacrylamide Gel with Switchable Trypsin Activity for Analysis of Proteins, ANALYTICAL CHEMISTRY, 2013, 第 6 作者
（68） Facile preparation of ordered mesoporous silica-carbon composite nanoparticles for glycan enrichment, CHEMICAL COMMUNICATIONS, 2013, 第 1 作者
（69） High specific phosphopeptides enrichment by titanium silicalite with post-treatment of desilication, ANALYTICAL METHODS, 2013, 第 6 作者
（70） Layer-by-layer assembly of multilayer polysaccharide coated magnetic nanoparticles for the selective enrichment of glycopeptides, CHEMICALCOMMUNICATIONS, 2013, 第 2 作者
（71） Facile synthesis of yolk-shell magnetic mesoporous carbon microspheres for efficient enrichment of low abundance peptides, NANOSCALE, 2013, 第 2 作者
（72） Analysis of human serum phosphopeptidome by a focused database searching strategy, JOURNAL OF PROTEOMICS, 2013, 第 5 作者
（73） Highly efficient extraction of biological samples with mesoporous adsorbents for protein and peptides analysis, the 5th international symposium on microchemistry and microsystems, 2013, 第 2 作者
（74） Selective capture of phosphopeptides by the hierarchical Ti-aluminophosphate-5 molecular sieves, the 5th international symposium on microchemistry and microsystems, 2013, 第 4 作者
（75） Highly Efficient Cleavage of Protein Corona on Fe3O4 Nanoparticles by a Magnetic Tryptic Nanorazor, the 5th international symposium on microchemistry and microsystems, 2013, 第 4 作者
（76） Global Screening of CK2 Kinase Substrates by an Integrated Phosphoproteomics Workflow, SCIENTIFIC REPORTS, 2013, 第 8 作者
（77） 修饰蛋白质组学分离鉴定新技术新方法, 科学通报, 2013, 第 3 作者
（78） A six-plex proteome quantification strategy reveals the dynamics of protein turnover, SCIENTIFIC REPORTS, 2013, 第 4 作者
（79） N-Terminal Labeling of Peptides by Trypsin-Catalyzed Ligation for Quantitative Proteomics, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2013, 第 7 作者
（80） Cell Nucleus Targeting for Living Cell Extraction of Nucleic Acid Associated Proteins with Intracellular Nanoprobes of Magnetic Carbon Nanotubes, ANALYTICAL CHEMISTRY, 2013, 第 3 作者
（81） Synthesis of branched PEG brushes hybrid hydrophilic magnetic nanoparticles for the selective enrichment of N-linked glycopeptides, CHEMICAL COMMUNICATIONS, 2012, 第 5 作者
（82） Highly Selective Extraction of Biological Samples with the Adsorbents of Mesoporous Materials, THE 27TH INTERNATIONAL SYMPOSIUM ON MICROSCALE BIOSEPARATIONS AND ANALYSES, 2012, 第 2 作者
（83） A new method for quantitative analysis of cell surface glycoproteome, PROTEOMICS, 2012, 第 5 作者
（84） Phosphoric acid functionalized mesoporous organo-silica (EPO) as the adsorbent for in situ enrichment and isotope labeling of endogenous phosphopeptides, CHEMICAL COMMUNICATIONS, 2012, 第 1 作者
（85） Isobaric Cross-Sequence Labeling of Peptides by Using Site-Selective Dimethylation Reaction, 38TH INTERNATIONAL SYMPOSIUM ON HIGH PERFORMANCE LIQUID PHASE SEPARATIONS AND RELATED TECHNIQUES, 2012, 第 1 作者
（86） A poly(ethylene glycol)-brush decorated magnetic polymer for highly specific enrichment of phosphopeptides, CHEMICAL SCIENCE, 2012, 第 2 作者
（87） Comprehensive analysis of the N and C terminus of endogenous serum peptides reveals a highly conserved cleavage site pattern derived from proteolytic enzymes, PROTEIN & CELL, 2012, 第 4 作者
（88） Recent advances of mesoporous materials in sample preparation, JOURNAL OF CHROMATOGRAPHY A, 2012, 第 2 作者
（89） Selective capture of phosphopeptides by hierarchical Ti-aluminophosphate-5 molecular sieves, CHEMICAL COMMUNICATIONS, 2012, 第 4 作者
（90） Preparation of phenyl-silica hybrid monolithic column with "one-pot" process for capillary liquid chromatography, JOURNAL OF CHROMATOGRAPHY A, 2012, 第 4 作者
（91） Highly Efficient Extraction of Cellular Nucleic Acid Associated Proteins in Vitro with Magnetic Oxidized Carbon Nanotubes, ANALYTICAL CHEMISTRY, 2012, 第 3 作者
（92） Isobaric Cross-Sequence Labeling of Peptides for Quantification of Proteins by MS/MS, ASIA OCEANIA HUMAN PROTEOME ORGANIZATION 6TH CONGRESS　, 2012, 第 1 作者
（93） Selective Capture of Phosphopeptides by the Hierarchical Tialuminophosphate-, ASIA OCEANIA HUMAN PROTEOME ORGANIZATION 6TH CONGRESS　, 2012, 第 4 作者
（94） Capture and Dimethyl Labeling of Glycopeptides on Hydrazide Beads for Quantitative Glycoproteomics Analysis, ANALYTICAL CHEMISTRY, 2012, 第 2 作者
（95） Size-selective Extraction of Biological Samples with Mesoporous Adsorbents for Protein and Peptides Analysis, 19TH INTERNATIONAL SYMPOSIUM EXHIBIT & WORKSHOPS ON ELECTRO- AND LIQUID PHASE-SEPARATION TECHNIQUES, 2012, 第 2 作者
（96） Isobaric cross-sequence labeling of peptides by using site-selective N-terminus dimethylation, CHEMICAL COMMUNICATIONS, 2012, 第 1 作者
（97） Preparation of capillary hybrid monolithic column with sulfonate strong cation exchanger for proteome analysis, JOURNAL OF CHROMATOGRAPHY A, 2012, 第 4 作者
（98） Mesoporous Materials for Sample Preparation in Bioanalysis, JONIT CONGRESS 2011 FINAL PROGRAM, 2011, 第 3 作者
（99） Mesoporous Materials for Sample Preparation in Bioanalysis, 26TH INTERNATIONAL SYMPOSIUM ON MICROSCALE BIOSEPARATIONS, 2011, 第 3 作者
（100） Size-Selective Enrichment of N-Linked Glycans Using Highly Ordered Mesoporous Carbon Material and Detection by MALDI-TOF MS, ANALYTICAL CHEMISTRY, 2011, 第 1 作者
（101） Highly Efficient Extraction of Serum Peptides by Ordered Mesoporous Carbon, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2011, 第 1 作者
（102） Solid-phase isotope labeling and multidimensional separation for proteome and phosphoproteome quantification, 37TH INTERNATIONAL SYMPOSIUM ON HIGH-PERFORMANCE LIQUID PHASE SEPARATIONS AND RELATED TECHNIQUES, 2011, 第 3 作者
（103） Polyhedral Oligomeric Silsesquioxane as a Cross-linker for Preparation of Inorganic-Organic Hybrid Monolithic Columns, ANALYTICAL CHEMISTRY, 2010, 第 4 作者
（104） Ti-SBA-15介孔材料用于磷酸化肽的高效富集, Applications of Ti-SBA-15 mesoporous material in high performance enrichment of phosphopeptides, 色谱, 2010, 第 2 作者
（105） Size-selective proteolysis on mesoporous silica-based trypsin nanoreactor for low-MW proteome analysis, CHEMICAL COMMUNICATIONS, 2010, 第 4 作者
（106） Coupling Strong Anion-Exchange Monolithic Capillary with MALDI-TOF MS for Sensitive Detection of Phosphopeptides in Protein Digest, ANALYTICAL CHEMISTRY, 2010, 第 4 作者
（107） The synthesis of Ti-hexagonal mesoporous silica for selective capture of phosphopeptides, CHEMICAL COMMUNICATIONS, 2010, 第 3 作者
（108） Profiling of N-linked Glycans from Human Serum with Oxidative Ordered Mesoporous Carbon, the 24th international symposium on micro-scale bioseparation,, 2009, 第 1 作者
（109） Enrichment of Phosphopeptides by a Novel SAX Capillary Monolithic Column for Sensitive MALDI-TOF MS Analysis, the 24th international symposium on micro-scale bioseparation,, 2009, 第 4 作者

   

（ 1 ） 血清内源性多肽定量分析方法的建立及其用于抑郁症疾病标记物的筛, 负责人, 国家任务, 2015-01--2017-12
（ 2 ） 蛋白糖基化修饰在胃癌多药耐药中特征性改变及其作用机制研究, 负责人, 国家任务, 2015-01--2018-12
（ 3 ） 基于非天然糖代谢标记的分泌蛋白质糖基化精细分析新方法研究, 负责人, 国家任务, 2018-01--2021-12
（ 4 ） 人类蛋白质组计划2.0-蛋白质组学驱动的精准 医学计划, 参与, 国家任务, 2020-12--2023-12

第四军医大学、大连医科大学、大连理工大学等单位

现指导学生

周家华  博士研究生  070302-分析化学  

刘莉  硕士研究生  070302-分析化学  

王佳怡  硕士研究生  070302-分析化学  

陈尧  博士研究生  070302-分析化学