基本信息
唐双焱 女 博导 中国科学院微生物研究所
电子邮件: tangsy@im.ac.cn
通信地址: 北京市朝阳区北辰西路1号院3号中科院微生物研究所
邮政编码: 100101
电子邮件: tangsy@im.ac.cn
通信地址: 北京市朝阳区北辰西路1号院3号中科院微生物研究所
邮政编码: 100101
招生信息
招生专业
071010-生物化学与分子生物学
招生方向
蛋白质工程,代谢工程,合成生物学
教育背景
2003-03--2006-08 首尔大学农业与生命科学学院 博士1995-09--1998-06 武汉大学生命科学学院 硕士1991-09--1995-06 武汉大学生命科学学院 学士
工作经历
工作简历
2011-08~现在, 中科院微生物研究所, 研究员2006-09~2010-12,宾夕法尼亚州立大学, 博士后2003-03~2006-08,首尔大学农业与生命科学学院, 博士1998-07~2003-02,云南省药品检验所, 主管药师1995-09~1998-06,武汉大学生命科学学院, 硕士1991-09~1995-06,武汉大学生命科学学院, 学士
教授课程
代谢工程天然药物研究方法学导论合成生物学与生物技术
专利与奖励
专利成果
( 1 ) 一种气相色谱质谱检测系统及方法, 2021, 第 4 作者, 专利号: CN112213436A( 2 ) 一株大肠埃希氏菌及其应用, 2020, 第 4 作者, 专利号: CN108949612B( 3 ) HpaBC基因、突变体及其应用, 2020, 第 3 作者, 专利号: CN110938606A( 4 ) 高产白藜芦醇突变体筛选方法的建立, 2018, 第 3 作者, 专利号: CN108117588A( 5 ) 一种高产莽草酸突变体的制备方法, 2017, 第 1 作者, 专利号: CN107446870A( 6 ) 一组亮氨酸脱氢酶及其编码基因和应用, 2017, 第 1 作者, 专利号: CN106906190A( 7 ) 一种高通量筛选产生1-脱氧野尻霉素菌株的方法, 2016, 第 1 作者, 专利号: CN105624181A( 8 ) 果胶裂解酶及其编码基因和应用, 2016, 第 2 作者, 专利号: CN105368811A( 9 ) 筛选具有将大量存在的人参皂苷转化为稀有人参皂苷的能力的菌株的方法, 2014, 第 3 作者, 专利号: CN104232671A( 10 ) 一种用于尿酸诱导目的蛋白表达的DNA片段及相关启动子与应用, 2014, 第 3 作者, 专利号: CN104073489A( 11 ) β-糖苷酶突变体及其编码基因与其在生产人参皂苷CK中的应用, 2014, 第 2 作者, 专利号: CN103805581A( 12 ) 一种AraC突变体蛋白及其应用, 2014, 第 2 作者, 专利号: CN103626852A
出版信息
发表论文
(1) Engineering and application of LacI mutants with stringent expressions, Microbial Biotechnology, 2024, (2) Designing glucose utilization "highway" for recombinant biosynthesis, Metabolic Engineering, 2023, (3) Engineering an SspB-mediated degron for novel controllable protein degradation, Metabolic Engineering, 2022, (4) Computational Redesign of a PETase for Plastic Biodegradation under Ambient Condition by the GRAPE Strategy, ACS CATALYSIS, 2021, 第 16 作者(5) Whole-Cell Biosensors Aid Exploration of Vanillin Transmembrane Transport, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2021, 第 11 作者(6) De Novo Biosynthesis of Chlorogenic Acid Using an Artificial Microbial Community, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2021, 第 11 作者(7) Computational Redesign of a PETase for Plastic Biodegradation under Ambient Condition by the GRAPE Strategy, ACS CATALYSIS, 2021, 第 16 作者(8) Whole-Cell Biosensors Aid Exploration of Vanillin Transmembrane Transport, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2021, 第 11 作者(9) De Novo Biosynthesis of Chlorogenic Acid Using an Artificial Microbial Community, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2021, 第 11 作者(10) Dynamic control of toxic natural product biosynthesis by an artificial regulatory circuit, METABOLIC ENGINEERING, 2020, 第 11 作者(11) Developing a highly efficient hydroxytyrosol whole-cell catalyst by de-bottlenecking rate-limiting steps, NATURE COMMUNICATIONS, 2020, 第 11 作者(12) CRISPRi/dCpf1-mediated dynamic metabolic switch to enhance butenoic acid production in Escherichia coli, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2020, 第 11 作者(13) Metabolic Engineering for Improved Curcumin Biosynthesis in Escherichia coli, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2020, 第 11 作者(14) Development of a highly efficient and specific l-theanine synthase, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2020, 第 11 作者(15) Dynamic control of toxic natural product biosynthesis by an artificial regulatory circuit, METABOLIC ENGINEERING, 2020, 第 11 作者(16) Developing a highly efficient hydroxytyrosol whole-cell catalyst by de-bottlenecking rate-limiting steps, NATURE COMMUNICATIONS, 2020, 第 11 作者(17) CRISPRi/dCpf1-mediated dynamic metabolic switch to enhance butenoic acid production in Escherichia coli, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2020, 第 11 作者(18) Metabolic Engineering for Improved Curcumin Biosynthesis in Escherichia coli, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2020, 第 11 作者(19) Development of a highly efficient and specific l-theanine synthase, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2020, 第 11 作者(20) Engineering the effector specificity of regulatory proteins for the in vitro detection of biomarkers and pesticide residues, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2019, 第 11 作者(21) Promiscuous enzymatic activity-aided multiple-pathway network design for metabolic flux rearrangement in hydroxytyrosol biosynthesis, NATURE COMMUNICATIONS, 2019, 第 11 作者(22) Engineering the effector specificity of regulatory proteins for the in vitro detection of biomarkers and pesticide residues, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2019, 第 11 作者(23) Promiscuous enzymatic activity-aided multiple-pathway network design for metabolic flux rearrangement in hydroxytyrosol biosynthesis, NATURE COMMUNICATIONS, 2019, 第 11 作者(24) Towards the construction of high-quality mutagenesis libraries, BIOTECHNOLOGY LETTERS, 2018, 第 11 作者(25) 人参果梗的化学成分研究, Chemical constituents from fruit pedicels of Panax ginseng, 中草药, 2018, 第 6 作者(26) Towards the construction of high-quality mutagenesis libraries, BIOTECHNOLOGY LETTERS, 2018, 第 11 作者(27) 人参果梗的化学成分研究, Chemical constituents from fruit pedicels of Panax ginseng, 中草药, 2018, 第 6 作者(28) 2-O-α-D-吡喃葡萄糖基抗坏血酸研究进展, Current Progress in Biosynthesis and Applications of 2-O-��-D-Glucopyranosyl-L-Ascorbic Acid, 广西科学, 2017, 第 2 作者(29) Design and application of a lactulose biosensor, SCIENTIFIC REPORTS, 2017, 第 11 作者(30) Biosensor-aided high-throughput screening of hyper-producing cells for malonyl-CoA-derived products, MICROBIAL CELL FACTORIES, 2017, 第 11 作者(31) Monitoring in vivo metabolic flux with a designed whole-cell metabolite biosensor of shikimic acid, BIOSENSORS & BIOELECTRONICS, 2017, 第 11 作者(32) Improving key enzyme activity in phenylpropanoid pathway with a designed biosensor, METABOLIC ENGINEERING, 2017, 第 11 作者(33) 碳水化合物结合结构域研究进展, Carbohydrate-binding modules: assisted polysaccharide recognition, 微生物学报, 2017, 第 2 作者(34) Genetic biosensors for small-molecule products: Design and applications in high-throughput screening, FRONTIERS OF CHEMICAL SCIENCE AND ENGINEERING, 2017, 第 11 作者(35) 2-O-α-D-吡喃葡萄糖基抗坏血酸研究进展, Current Progress in Biosynthesis and Applications of 2-O-��-D-Glucopyranosyl-L-Ascorbic Acid, 广西科学, 2017, 第 2 作者(36) Design and application of a lactulose biosensor, SCIENTIFIC REPORTS, 2017, 第 11 作者(37) Biosensor-aided high-throughput screening of hyper-producing cells for malonyl-CoA-derived products, MICROBIAL CELL FACTORIES, 2017, 第 11 作者(38) Monitoring in vivo metabolic flux with a designed whole-cell metabolite biosensor of shikimic acid, BIOSENSORS & BIOELECTRONICS, 2017, 第 11 作者(39) Improving key enzyme activity in phenylpropanoid pathway with a designed biosensor, METABOLIC ENGINEERING, 2017, 第 11 作者(40) 碳水化合物结合结构域研究进展, Carbohydrate-binding modules: assisted polysaccharide recognition, 微生物学报, 2017, 第 2 作者(41) Genetic biosensors for small-molecule products: Design and applications in high-throughput screening, FRONTIERS OF CHEMICAL SCIENCE AND ENGINEERING, 2017, 第 11 作者(42) Research Progress on The Mechanism of Intramolecular Chaperone, PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 2016, 第 4 作者(43) Putative Nonribosomal Peptide Synthetase and Cytochrome P450 Genes Responsible for Tentoxin Biosynthesis in Alternaria alternata ZJ33, TOXINS, 2016, 第 5 作者(44) Directed evolution of leucine dehydrogenase for improved efficiency of l-tert-leucine synthesis, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 第 11 作者(45) 分子内分子伴侣机制的研究进展, Research Progress on The Mechanism of Intramolecular Chaperone, PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 2016, 第 4 作者(46) Engineering a Carbohydrate-processing Transglycosidase into Glycosyltransferase for Natural Product Glycodiversification, SCIENTIFIC REPORTS, 2016, 第 11 作者(47) 人参茎叶总皂苷酶水解产物成分研究, Chemical Constituents of Enzymatic Hydrolysate of Total Ginsenoside from Stems and Leaves of Panax ginseng, 天然产物研究与开发, 2016, 第 5 作者(48) Research Progress on The Mechanism of Intramolecular Chaperone, PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 2016, 第 4 作者(49) Putative Nonribosomal Peptide Synthetase and Cytochrome P450 Genes Responsible for Tentoxin Biosynthesis in Alternaria alternata ZJ33, TOXINS, 2016, 第 5 作者(50) Directed evolution of leucine dehydrogenase for improved efficiency of l-tert-leucine synthesis, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 第 11 作者(51) 分子内分子伴侣机制的研究进展, Research Progress on The Mechanism of Intramolecular Chaperone, PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 2016, 第 4 作者(52) Engineering a Carbohydrate-processing Transglycosidase into Glycosyltransferase for Natural Product Glycodiversification, SCIENTIFIC REPORTS, 2016, 第 11 作者(53) 人参茎叶总皂苷酶水解产物成分研究, Chemical Constituents of Enzymatic Hydrolysate of Total Ginsenoside from Stems and Leaves of Panax ginseng, 天然产物研究与开发, 2016, 第 5 作者(54) Improving the thermoactivity and thermostability of pectate lyase from Bacillus pumilus for ramie degumming, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2015, 第 11 作者(55) Design and Application of a Novel High-throughput Screening Technique for 1-Deoxynojirimycin, SCIENTIFIC REPORTS, 2015, 第 7 作者(56) Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis, METABOLIC ENGINEERING, 2015, 第 9 作者(57) Development of a novel uric-acid-responsive regulatory system in Escherichia coli, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2015, 第 11 作者(58) Improving the thermoactivity and thermostability of pectate lyase from Bacillus pumilus for ramie degumming, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2015, 第 11 作者(59) Design and Application of a Novel High-throughput Screening Technique for 1-Deoxynojirimycin, SCIENTIFIC REPORTS, 2015, 第 7 作者(60) Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis, METABOLIC ENGINEERING, 2015, 第 9 作者(61) Development of a novel uric-acid-responsive regulatory system in Escherichia coli, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2015, 第 11 作者(62) 西洋参总皂苷酶水解产物成分研究, Chemical Constituents of Enzymatic Hydrolysate of Total Ginsenoside Extract of Panax quinquefolium, 天然产物研究与开发, 2014, 第 5 作者(63) 西洋参总皂苷酶水解产物成分研究, Chemical Constituents of Enzymatic Hydrolysate of Total Ginsenoside Extract of Panax quinquefolium, 天然产物研究与开发, 2014, 第 5 作者(64) Screening for Enhanced Triacetic Acid Lactone Production by Recombinant Escherichia coli Expressing a Designed Triacetic Acid Lactone Reporter, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2013, 第 1 作者(65) Screening for Enhanced Triacetic Acid Lactone Production by Recombinant Escherichia coli Expressing a Designed Triacetic Acid Lactone Reporter, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2013, 第 1 作者(66) 高通量筛选工具的设计与应用, Design and application of high-throughput screening tools: a review, 生物工程学报, 2012, 第 1 作者(67) 高通量筛选工具的设计与应用, Design and application of high-throughput screening tools: a review, 生物工程学报, 2012, 第 1 作者(68) Design and Application of a Mevalonate-Responsive Regulatory Protein, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2011, 第 1 作者(69) Design and Application of a Mevalonate-Responsive Regulatory Protein, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2011, 第 1 作者(70) Elucidating residue roles in engineered variants of AraC regulatory protein, PROTEIN SCIENCE, 2010, 第 1 作者(71) Elucidating residue roles in engineered variants of AraC regulatory protein, PROTEIN SCIENCE, 2010, 第 1 作者(72) AraC regulatory protein mutants with altered effector specificity, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 第 1 作者(73) AraC regulatory protein mutants with altered effector specificity, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 第 1 作者(74) Enhancing thermostability of maltogenic amylase from Bacillus thermoalkalophilus ET2 by DNA shuffling, FEBS JOURNAL, 2006, 第 1 作者(75) Contribution of W229 to the transglycosylation activity of 4-��-glucanotransferase from Pyrococcus furiosus, BBA - PROTEINS AND PROTEOMICS, 2006, 第 1 作者(76) Enhancing thermostability of maltogenic amylase from Bacillus thermoalkalophilus ET2 by DNA shuffling, FEBS JOURNAL, 2006, 第 1 作者(77) Contribution of W229 to the transglycosylation activity of 4-��-glucanotransferase from Pyrococcus furiosus, BBA - PROTEINS AND PROTEOMICS, 2006, 第 1 作者(78) Thermostable and alkalophilic maltogenic amylase of Bacillus thermoalkalophilus ET2 in monomer-dimer equilibrium, BIOCATALYSIS AND BIOTRANSFORMATION, 2005, (79) Thermostable and alkalophilic maltogenic amylase of Bacillus thermoalkalophilus ET2 in monomer-dimer equilibrium, BIOCATALYSIS AND BIOTRANSFORMATION, 2005,
科研活动
科研项目
( 1 ) 高带宽单细胞微反应实时动态观测介观物镜研制及应用, 参与, 中国科学院计划, 2022-01--2023-12( 2 ) 药用多肽绿色生物制造关键技术与产业示范, 参与, 国家任务, 2021-12--2025-11( 3 ) 合成塑料解聚酶的人工设计与定向进化, 负责人, 国家任务, 2020-01--2023-12( 4 ) 转录调控蛋白配体结合口袋可塑性研究及小分子生物传感器的设计, 负责人, 国家任务, 2020-01--2023-12( 5 ) 甜菊糖苷和肌醇及其衍生物的生物合成技术研发, 负责人, 境内委托项目, 2019-11--2024-10( 6 ) 调控元器件的创制 与功能表征, 负责人, 国家任务, 2019-07--2024-06( 7 ) 白藜芦醇生物合成技术的开发, 负责人, 境内委托项目, 2016-10--2018-10( 8 ) 羟基酪醇生物合成技术的开发, 负责人, 境内委托项目, 2016-10--2018-10( 9 ) 广西特色非粮生物质资源化利用技术开发专项, 参与, 地方任务, 2016-01--2019-12( 10 ) 淀粉深加工产品开发研究, 负责人, 境内委托项目, 2015-07--2018-05( 11 ) 改造调控蛋白LacI建立二糖分子高通量筛选方法, 负责人, 国家任务, 2015-01--2018-12( 12 ) 一种beta-糖苷酶突变体及其编码基因与其在生产人参皂苷CK中的应用, 负责人, 境内委托项目, 2014-09--2029-09( 13 ) 抗生素的发掘及其合成生物学研究, 负责人, 中国科学院计划, 2013-09--2016-08( 14 ) 底盘细胞中前体化合物检测技术的建立, 负责人, 国家任务, 2013-01--2017-12( 15 ) 脱胶酶资源的挖掘、分子改造和酶法脱胶新工艺的开发, 负责人, 中国科学院计划, 2013-01--2015-12( 16 ) 改造调控蛋白设计D-塔格糖信号分子及其功能验证, 负责人, 国家任务, 2012-01--2015-12