基本信息
王钦宏  男  博导  中国科学院天津工业生物技术研究所
电子邮件: wang_qh@tib.cas.cn
通信地址: 天津空港经济区西七道32号
邮政编码: 300308

招生信息

   
招生专业
081703-生物化工
071010-生物化学与分子生物学
招生方向
进化与代谢工程
代谢工程

教育背景

1999-09--2003-03   中国科学院微生物研究所   理学博士
1996-09--1999-04   郑州粮食学院   工学硕士
1992-09--1996-06   郑州粮食学院   工学学士

工作经历

   
工作简历
2009-05~现在, 中国科学院天津工业生物技术研究所, 研究员/博士研究生
2005-06~2009-04,California Institute of Technology, Postdoctoral Scholar/Research Scientist
2004-01~2005-06,University of Oklahoma, Postdoectoral Research Associate
1999-09~2003-03,中国科学院微生物研究所, 理学博士
1996-09~1999-04,郑州粮食学院, 工学硕士
1992-09~1996-06,郑州粮食学院, 工学学士
社会兼职
2016-12-31-今,Scientific Reports, 编委
2012-10-01-今,生物工程学报, 编委

教授课程

工业生物技术——生物技术的第三次浪潮系列讲座

专利与奖励

   
奖励信息
(1) 国务院政府特殊津贴, , 国家级, 2018
(2) 天津市优秀党员, , 市地级, 2018
(3) 工业合成生物创新团队, , 市地级, 2017
(4) 天津市五一劳动奖章, , 市地级, 2016
(5) 天津市科技系统优秀青年, , 市地级, 2013
(6) 中国科学院“引进杰出人才计划(****), , 院级, 2009
专利成果
( 1 ) 生产多巴胺的大肠杆菌重组菌株及其构建方法与应用, 2021, 第 1 作者, 专利号: 202110180874.X

( 2 ) 生产3-脱氢莽草酸大肠杆菌重组菌株及其构建方法与应用, 2021, 第 2 作者, 专利号: ZL 201711002831.2

( 3 ) 生产左旋多巴大肠杆菌重组菌株及其构建方法与应用, 2020, 第 2 作者, 专利号: 201711003046.9

( 4 ) 一种从发酵液中分离提取原儿茶酸的方法, 2020, 第 4 作者, 专利号: 202011071195.0

( 5 ) 一种基因定点突变方法及其胁迫抗性育种应用, 2020, 第 5 作者, 专利号: 202010310599.4

( 6 ) 一种基于儿茶酸的环氧树脂的合成和应用, 2019, 第 3 作者, 专利号: 201911329150.6

( 7 ) 一种基于滚环复制的质粒线性化方法及在感受态转化中的应用, 2019, 第 2 作者, 专利号: 201911087486.6

( 8 ) 一种低温碱性蛋白酶及其制备方法, 2019, 第 3 作者, 专利号: ZL 201710117439.6

( 9 ) 酿酒酵母及其育种方式及工业化发酵生产乙醇的应用, 2019, 第 1 作者, 专利号: ZL 201711020313.3

( 10 ) 一种新型产糖醇酵母菌株基因组重排技术的构建及其应用, 2019, 第 1 作者, 专利号: ZL 201510070962.9

( 11 ) 一种用于液滴荧光检测的显微镜集成光路系统, 2019, 第 4 作者, 专利号: ZL 201510739868.8

( 12 ) 基于液滴微流控芯片的毕赤酵母高通量筛选方法, 2019, 第 2 作者, 专利号: 201910212336.7

( 13 ) 一株利用棕榈油生产鼠李糖脂生物表面活性剂的铜绿假单胞菌及其应用, 2019, 第 1 作者, 专利号: ZL 201410480597.4

( 14 ) 生产奎尼酸相关的大肠杆菌重组菌株及其构建方法与应用, 2018, 第 1 作者, 专利号: 201810490824.X

( 15 ) 一组耐螯合剂乙二胺四乙酸的重组碱性木聚糖酶及其构建方法, 2018, 第 3 作者, 专利号: ZL 201410115520.7

( 16 ) 一种使用液滴微流控芯片的高通量筛选设备, 2018, 第 1 作者, 专利号: ZL 201720740015.0

( 17 ) 一株生产原儿茶酸的大肠杆菌基因工程菌及其构建方法与应用, 2017, 第 1 作者, 专利号: 201711390790.9

( 18 ) 发酵法生产酪氨酸的大肠杆菌及其构建方法与应用, 2017, 第 1 作者, 专利号: 201711343436.0

( 19 ) 一种基于生物传感器的高通量筛选方法, 2017, 第 1 作者, 专利号: 201710973957.8

( 20 ) 一种应答3-脱氢莽草酸的生物传感器及其应用, 2017, 第 3 作者, 专利号: 201710503939.3

( 21 ) 一种使用液滴微流控芯片的高通量筛选设备, 2017, 第 1 作者, 专利号: 201710486937.8

( 22 ) 一种简便高效的基因编辑方法, 2017, 第 4 作者, 专利号: ZL 201410148355.5

( 23 ) 一种提高大肠杆菌积累丙酮酸的方法, 2017, 第 1 作者, 专利号: 201710128712.5

( 24 ) 一种基于双酶偶联高通量检测微生物生产异戊醇的新方法, 2016, 第 1 作者, 专利号: ZL 201310133868.4

( 25 ) 一种程序化控制的驯化筛选培养设备, 2014, 第 1 作者, 专利号: ZL 201320461836.2

出版信息

   
发表论文
(1) Genomic, transcriptomic, and metabolic characterizations of Escherichia coli adapted to branched-chain higher alcohol tolerance., Applied Microbiology And Biotechnology, 2020, 通讯作者
(2) Design and Reconstruction of Regulatory Parts for Fast-frowing Vibrio natriegens Synthetic Biology., Acs Synthetic Biology, 2020, 通讯作者
(3) Biosensor-enabled droplet microfluidic system for the rapid screening of 3-dehydroshikimic acid produced in Escherichia coli., Journal Of Industrial Microbiology & Biotechnology, 2020, 通讯作者
(4) Development of a Simple Colorimetric Assay for Determination of the Isoamyl Alcohol-Producing Strain., Applied Biochemistry and Biotechnology, 2020, 通讯作者
(5) Discovery and identification of medium-chain fatty acid responsive promoters in Saccharomyces cerevisiae., Engineering In Life Sciences, 2020, 通讯作者
(6) Metabolic engineering of Escherichia coli for polyamides monomer δ-valerolactam production from feedstock lysine., Applied Microbiology And Biotechnology, 2020, 通讯作者
(7) Development of a synthetic 3-dehydroshikimate biosensor in Escherichia coli for metabolite monitoring and genetic screening., ACS Synthetic Biology, 2019, 通讯作者
(8) Development and genomic elucidation of hybrid yeast with improved glucose-xylose co-fermentation at high temperature., FEMS Yeast Research, 2019, 通讯作者
(9) QTL analysis reveals genomic variants linked to high‑temperature fermentation performance in the industrial yeast., Biotechnology for Biofuels, 2019, 通讯作者
(10) Systematically redesigning and optimizing the expression of D-lactate dehydrogenase efficiently produces high-optical-purity D-lactic acid in Saccharomyces cerevisiae., Biochemical Engineering Journal, 2019, 通讯作者
(11) Distinct Proteome Remodeling of Industrial Saccharomyces cerevisiae in Response to Prolonged Thermal Stress or Transient Heat Shock., Journal of Protome Research, 2018, 通讯作者
(12) Metabolic and genomic characterization of stress tolerant industrial Saccharomyces cerevisiae strains from TALENs-assisted multiplex editing., FEMS Yeast Research, 2018, 通讯作者
(13) Expanding lysine industry: industrial biomanufacturing of lysine and its derivatives., Journal of Industrial Microbiology and Biotechnology, 2018, 通讯作者
(14) A pH-stable, detergent and chelator resistant type I pullulanase from Bacillus pseudofirmus 703 with high catalytic efficiency. International, Journal of Biological Macromolecules, 2018, 第 4 作者
(15) An economically and environmentally acceptable synthesis of chiral drug intermediate L-pipecolic acid from biomass-derived lysine via artificially engineered microbes., Journal of Industrial Microbiology and Biotechnology, 2018, 通讯作者
(16) Expanding the repertoire of aromatic chemicals by microbial production., Journal of Chemical Technology and Biotechnology, 2018, 通讯作者
(17) Enhanced 5-aminovalerate production in Escherichia coli from L-lysine with ethanol and hydrogen peroxide addition., Journal of Chemical Technology and Biotechnology, 2018, 通讯作者
(18) Comprehensive evaluation of untargeted metabolomics data processing software in feature detection, quantification and discriminating marker selection., Analytica Chimica Acta, 2018, 通讯作者
(19)  Li Z, Li Y, Chen W, Cao Q, Guo Y, Wan N, Jiang X, Tang YJ, Wang Q, Shui W. Integrating MS1 and MS2 Scans in High-Resolution Parallel Reaction Monitoring Assays for Targeted Metabolite Quantification and Dynamic 13C-Labeling Metabolism Analysis., Analytical Chemistry, 2017, 第 9 作者
(20) High copy and stable expression of the xylanase XynHB in Saccharomyces cerevisiae by rDNA-mediated integration., Scientific Reports, 2017, 第 2 作者
(21) Synthetic biology for manufacturing chemicals: constraints drive the use of non-conventional microbial platforms., Applied Microbiology and Biotechnology, 2017, 第 2 作者
(22) Characterization of aromatic residue-controlled protein retention in the endoplasmic reticulum of Saccharomyces cerevisiae., Journal of Biological Chemistry, 2017, 第 7 作者
(23) Designing and Creating a Synthetic Omega Oxidation Pathway in Saccharomyces cerevisiae Enables Production of Medium-Chain α, ω-Dicarboxylic Acids., Frontiers in Microbiology, 2017, 通讯作者
(24) Fluorescence-activated droplet sorting of lipolytic microorganisms using a compact optical system., Lab on a Chip, 2017, 第 8 作者
(25) High-level expression of improved thermo-stable alkaline xylanase variant in Pichia Pastoris through codon optimization, multiple gene insertion and high-density fermentation., Scientific Reports, 2016, 第 3 作者
(26) . High production of fatty alcohols in Escherichia coli with fatty acid starvation., Microbial Cell Factories, 2016, 通讯作者
(27) An NGS-Independent Strategy for Proteome-Wide Identification of Single Amino Acid Polymorphisms by Mass Spectrometry., Analytical Chemistry, 2016, 通讯作者
(28) Truncation of the unique N-terminal domain improved the thermos-stability and specific activity of alkaline α-amylase Amy703., Scientific Reports, 2016, 第 2 作者
(29) PCR-Based Seamless Genome Editing with High Efficiency and Fidelity in Escherichia coli., PLoS One, 2016, 通讯作者
(30) Understanding the Mechanism of Thermotolerance Distinct From Heat Shock Response Through Proteomic Analysis of Industrial Strains of Saccharomyces cerevisiae, Molecular & Cellular Proteomics 2015, 2015, 通讯作者
(31) TALENs-Assisted Multiplex Editing for Accelerated Genome Evolution To Improve Yeast Phenotypes., ACS Synthetic Biology, 2015, 通讯作者
(32) Rhamnolipid production, characterization and fermentation scale-up by Pseudomonas aeruginosa with plant oils., Biotechnology Letters, 2015, 通讯作者
(33) Structural Insight into and Mutational Analysis of Family 11 Xylanases: Implications for Mechanisms of Higher pH Catalytic Adaptation., PLoS One, 2015, 第 4 作者
(34) Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production., Microbial Cell Factories, 2015, 通讯作者
(35) Engineering catechol 1, 2-dioxygenase by design for improving the performance of the cis, cis-muconic acid synthetic pathway in Escherichia coli., Scientific Reports, 2015, 通讯作者
(36) Functional role of β domain in the Thermoanaerobacter tengcongensis glucoamylase.,  Applied Microbiology and Biotechnology, 2014, 第 4 作者
(37) Characterization of the sugar alcohol-producing yeast Pichia anomala.,  Journal of Industrial Microbiology and Biotechnology, 2014, 通讯作者
(38) Engineering the biosynthesis of novel rhamnolipid biosurfactants in Escherichia coli.,  Journal of Applied Microbiology, 2014, 通讯作者
(39) A double-enzyme-coupled assay for high-throughput screening of succinic acid-producing strains., Journal of Applied Microbiology , 2013, 通讯作者
(40) Directed evolution of cytochrome P450 for sterol epoxidation. , Biotechnology Letters , 2013, 通讯作者
(41) A simple colorimetric method for detection of 16,17-epoxysterol compounds., Chemistry Letters, 2013, 通讯作者
(42) Branched-chain higher alcohols, Advances in Biochemical Engineering/ Biotechnology, 2012, 通讯作者
(43) Ionic liquids-based hydrolysis of Chlorella biomass for fermentable sugars., Bioresource Technology, 2012, 通讯作者
(44) High-throughput screening and characterization of xylose-utilizing and ethanol tolerant thermophilic bacteria for bioethanol production., Journal of Applied Microbiology, 2011, 通讯作者
(45) Hydrolysis of Chlorella biomass for fermentable sugars in the presence of HCl and MgCl2, Bioresource Technology, 2011, 通讯作者
(46) The alcohol dehydrogenase system in the xylose-fermenting yeast Candida maltosa, Plos One, 2010, 通讯作者
(47) Engineering bacteria for production of rhamnolipid as agent for enhanced oil recovery, Biotechnology and Bioengineering , 2007, 第 1 作者
(48) MukEF is required for stable association of MukB with the chromosome, Journal of Bacteriology, 2007, 第 2 作者
(49) Chromosome condensation in the absence of non-SMC subunits of MukBEF, Journal of Bacteriology, 2006, 第 1 作者
(50) C-terminus mutations of Acremonium chrysogenum deacetoxyl/deacetylcephalosporin C synthesis with improved activity toward penicillin analogs, FEMS Microbiology Letters, 2005, 第 3 作者
(51) Metabolic engineering of Torulopsis glabrata for improved pyruvate production, Enzyme and Microbial Technology, 2005, 第 1 作者
(52) Purification, characterization, cloning and expression of pyruvate decarboxylase from Torulopsis glabrata, Journal of Biochemistry (Tokyo), 2004, 第 1 作者
(53) Screening of pyruvate-producing yeast and effect of nutritional conditions on pyruvate production, Letters in Applied Microbiology, 2002, 第 1 作者
(54) 
发表著作
( 1 ) 《2017高技术发展报告》-工业生物制造技术产业化新进展, 科学出版社, 2017-09, 第 1 作者

科研活动

   
科研项目
( 1 ) 人工合成细胞工厂, 参与, 国家任务, 2011-01--2015-08
( 2 ) 液滴微流控微生物高通量筛选装备研制, 负责人, 地方任务, 2014-10--2017-09
( 3 ) 糖醇合成与功能性糖醇的研制, 参与, 国家任务, 2012-01--2015-12
( 4 ) 生物质液体燃料的高温生物炼制, 参与, 国家任务, 2012-01--2015-12
( 5 ) 天然木糖利用麦芽糖假丝酵母葡萄糖阻遏效应及其调控机理研究, 负责人, 国家任务, 2013-01--2016-12
( 6 ) 绿色生物工艺研发与应用, 负责人, 中国科学院计划, 2013-01--2015-12
( 7 ) 生物表面活性剂鼠李糖脂的双相发酵技术, 参与, 国家任务, 2014-01--2016-12
( 8 ) 多位点基因组编辑改善酿酒酵母多重胁迫耐性及机理研究, 负责人, 国家任务, 2015-01--2018-12
( 9 ) 多尺度微生物单细胞高通量筛选系统研制, 负责人, 中国科学院计划, 2018-01--2019-12
( 10 ) 原料药和药物中间体的生物合成(子课题:治疗老年痴呆原料药左旋多巴生物合成及中试生产), 参与, 中国科学院计划, 2017-01--2018-12
( 11 ) 液滴微流控高通量选育高效产纤维素酶的微生态菌株, 负责人, 企业委托, 2017-02--2018-08
( 12 ) 螺旋霉素工业菌株改造提升, 负责人, 企业委托, 2017-10--2019-09
( 13 ) 原儿茶酸、红景天苷、灯盏花素合成生物学研究, 负责人, 国家任务, 2018-01--2020-12
( 14 ) 变革性的化学品生物制造技术研发及示范(子任务名称:航空航天用环氧树脂生物合成制造及应用测评), 负责人, 中国科学院计划, 2019-01--2020-12
( 15 ) 合成生物制造发展战略研究, 负责人, 中国科学院计划, 2019-01--2019-12
( 16 ) 生物发酵法生产左旋多巴菌种, 负责人, 企业委托, 2017-10--2037-10
( 17 ) 发酵法生产3-脱氢莽草酸菌种, 负责人, 企业委托, 2017-12--2027-12
参与会议
(1)High efficient production of aromatic chemicals in metabolically engineering Escherichia coli.   2017-05-01
(2)Development and Optimization of droplet microfluidic-based high throughput screening system for industrial microbiology.    2014-07-20
(3)Engineering Escherichia coli for adipic acid production.    2012-10-28
(4)Xylitol production by evolutionarily engineered Candida maltosa.   2011-07-24
(5)功能糖醇的生物制造   功能糖研究开发与应用技术专题研讨会   王钦宏,江宁,贺鹏   2011-06-23
(6)Isolation and Characterization of novel xylose-utilizing and ethanol tolerant thermophilic bacteria.   2010-05-23
(7)Developing Highly Sensitive Micro-Biosensors for in-situ Monitoring Mercury and Chromium (VI) Contaminants by Genetically-evolving and Computer-designing Metal-binding Proteins.   2009-04-20
(8)Engineering mercury biosensor by synthesis and evolution.   2009-01-18