Dunming Zhu， Tianjin Institute of Industrial Biotechnology
Email: zhu_dm@tib.cas.cn
Telephone: 022-84861962
Address: 天津空港经济区西七道32号
Postcode: 300308
Research Areas
Education
1993 Ph.D., Organic Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, China.
1990 M.S., Organic Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, China.
1987 B.S., Polymer Chemistry, University of Science & Technology of China, Hefei, China.
Experience
2008-present Professor, National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China
2004-2008 Research Professor, Department of Chemistry, Southern Methodist University, Dallas, TX 75275, USA
2000-2004 Staff Scientist and Head of Chemistry Division, BioCatalytics, Inc., Pasadena, CA, USA
1996-1999 Research Associate and Lab Manager, University of Houston, Houston, TX, USA
1994-1996 Postdoctoral Associate, Southern Methodist University, Dallas, Texas, USA
1993-1994 Research Scientist, Shanghai Institute of Organic Chemistry, Shanghai, China
Honors & Distinctions
2009 Named “Thousand Talents Program” Distinguished Expert by Tianjin Municipal Government.
2008 Tetrahedron: Asymmetry Most Cited Paper 2005-2008 Award by Elsevier Ltd, Oxford, UK (2 papers).
2008 Selected to “Hundred Talents Program” by Chinese Academy of Sciences.
2007 Tetrahedron: Asymmetry Most Cited Paper 2004-2007 Award by Elsevier Ltd, Oxford, UK (1 paper).
1997 Third Class Award (shared) in Natural Sciences by Chinese National Commission of Science and Technology.
1995 First Class Award (shared) in Natural Sciences by Chinese Academy of Sciences.
Publications
Papers
1. L. Zhao, W. Liu, X. Chen, M. Wang, Q. Wu, D. Zhu, “Engineering the co-enzyme specificity of Symbiobacterium therophilum meso-diaminopimelate dehydrogenase”, 2016,
2. Q. Chen, X. Chen, Y. Cui, J. Ren, Y. Du, J Feng, Q. Wu, D. Zhu, “D-Threonine aldolase from Delftia sp. RIT313, a promising biocatalyst for the synthesis of aromatic β-hydroxy-α-amino acids”, 2016,
3.
4. P. Yao, Y. Cui, S. Yu, Y. Du, J. Feng, Q. Wu, D. Zhu, “Efficient biosynthesis of (R)- or (S)-2-hydroxybutyrate from L-threonine through a synthetic biology approach”, 2016, 358, 2923-2928.
5. Q. Chen, L. Han, X. Chen, Y. Cui, J. Feng, Q. Wu, D. Zhu, “A sialic acid aldolase from Peptoclostridium difficile NAP08 with 4-hydroxy-2-oxo-pentanoate aldolase activity”, 2016, 92, 99-106.
6. 杜允成，董文玥，姜进举，陈启佳，冯进辉，吴洽庆，朱敦明, “一种来源于Burkholderia phytofirmans PsJN的ω-转氨酶的表达纯化及性质分析”, 生物工程学报, 2016, 32, 912-926.
7. 张雅飞, 冯进辉, 李键煚, 王 玉, 王 敏, 吴洽庆, 朱敦明, “酶催化奎宁酮不对称还原合成(S)-3-奎宁醇”, 应用与环境生物学报, 2016,
8. 赵 艳, 张大龙, 李键煚, 冯进辉, 鲍锦库, 吴洽庆, 朱敦明, “青霉素G酰化酶突变体酶催化合成顺式头孢丙烯工艺优化”, 应用与环境生物学报, 2016, 22, 363-370.
9. G. Li, P. Yao, P. Cong, J. Ren, L. Wang, J. Feng, P. C.K. Lau, Q. Wu, D. Zhu, “New recombinant cyclohexylamine oxidase variants for deracemization of secondary amines by orthogonally assaying designed mutants with structurally diverse substrates”, Scientific Reports, 2016, 6, 24973; doi: 10.1038/srep24973
10. S. Yu, P. Yao, J. Li, J. Ren, J. Yuan, J. Feng, M. Wang, Q. Wu, D. Zhu, “Enzymatic Synthesis of 3-Hydroxypropionic Acid at High Productivity by Using Free or Immobilized cells of Recombinant Escherichia coli”, Journal of Molecular Catalysis B. Enzymatic, 2016, 129, 37-42.
11. J. Ren, P. Yao, S. Yu, W. Dong, Q. Chen, J. Feng, Q. Wu, D. Zhu, “An Unprecedented Effective Enzymatic Carboxylation of Phenols”, ACS Catalysis, 2016, 5, 564-567 （X-MOL平台报道）.
12. Y. Duan, P. Yao, Y. Du, J. Feng, Q. Wu, D. Zhu, “Synthesis of α,β-unsaturated esters via a chemo-enzymatic chain elongation approach by combining carboxylic acid reduction and Wittig reaction”, Beilstein Journal of Organic Chemistry, 2015, 11, 2245–2251.
13. D. Zhang, X. Chen, J. Chi, J. Feng, Q. Wu, D. Zhu, “Semi–Rational Engineering a Carbonyl Reductase to Enhance the Enzyme Activity and Enantioselectivity for the Reduction of b-Amino Ketones”, ACS Catalysis, 2015, 5, 2452-2457.
14. P. Yao, L. Wang, J. Yuan, L. Cheng, R. Jia, M. Xie, J. Feng, M. Wang, Q. Wu, D. Zhu, “Novel Biosynthesis of Ethyl (R)-3-Hydroxyglutarate through Halohydrin Dehalogenase and Nitrilase Bienzymatic Cascade”, ChemCatChem, 2015, 7, 1438-1444 （封面文章）.
15. X. Chen, T. Mei, Y. Cui, Q. Chen, X. Liu, J. Feng, Q. Wu, D. Zhu, “Highly Effective Synthesis of Optically Pure (S)-1-Phenyl-1,2-ethanediol by a Self-Sufficient Whole Cell Biocatalyst”, ChemistryOpen, 2015, 4, 483-488.
16. W. Liu, R.-T. Guo, X. Chen, Z. Li, X. Gao, C.-H. Huang, Q. Wu, J. Feng, D. Zhu, “Structural Analysis Reveals the Substrate Binding Mechanism for the Expanded Substrate Specificity of Mutant meso-Diaminopimelate Dehydrogenase”, ChemBioChem, 2015, 16, 924-929.
17. D. Zhang, X. Chen, R. Zhang, P. Yao, Q. Wu, D. Zhu, “Development of β-Amino Acid Dehydrogenase for the Synthesis of β-Amino Acids via Reductive Amination of β-Keto Acids”, ACS Catalysis, 2015, 5, 2220-2224.
18. P. Yao, J. Li, J. Yuan, C. Han, X. Liu, J. Feng, Q. Wu, D. Zhu, “Enzymatic synthesis of a key intermediate for rosuvastatin by nitrilase-catalyzed hydrolysis of ethyl (R)-4-cyano-3-hydroxy butyate at high substrate concentration”, ChemCatChem, 2015, 7, 271-275.
19. Y. Duan, P. Yao, X. Chen, X. Liu, R. Zhang, J. Feng, Q. Wu, D. Zhu, “Exploring the synthetic applicability of a new carboxylic acid reductase from Segniliparus rotundus DSM 44985”, Journal of Molecular Catalysis B. Enzymatic, 2015, 115, 1-7.
20. C. Han, P. Yao, J. Yuan, Y. Duan, J. Feng, M. Wang, Q. Wu, D. Zhu, “Nitrilase-catalyzed hydrolysis of 3-aminopropionitrile at high concentration with a tandem reaction strategy for shifting the reaction to β-alanine formation”, Journal of Molecular Catalysis B. Enzymatic, 2015, 115, 113-118.
21. 赵雷明，刘卫东，陈曦，王敏，冯进辉，吴洽庆，朱敦明,“嗜热共生杆菌内消旋-2,6-二氨基庚二酸脱氢酶中Y76对辅酶偏好性影响”，生物工程学报, 2015, 31(7), 1108−1118.
22. 王雷，袁京，姚培圆，程丽华，解美仙，贾荣荣，冯进辉，王敏，吴洽庆，朱敦明，“一种来源于运动替斯崔纳菌KA081020-065的新型卤醇脱卤酶的表达纯化及性质分析”，生物工程学报, 2015, 31(5), 659−669.
23. 张航，陈曦，冯进辉，鲍锦库，吴洽庆，朱敦明，“一种发现具有催化活性和立体选择性羰基还原酶的高通量筛选方法”，生物工程学报, 2015, 31(2), 220-230.
24. J. Jiang, X. Chen, D. Zhang, Q. Wu, D. Zhu, “Characterization of (R)-selective amine transaminases identified by in silico motif sequence blast”, Applied Microbiology and Biotechnology, 2015, 99, 2613-2621.
25. D. Zhang, R. Zhang, J. Zhang, L. Chen, C. Zhao, W. Dong, Q. Zhao, Q. Wu, D. Zhu, “Engineering a hydroxysteroid dehydrogenase to improve its soluble expression for the asymmetric reduction of cortisone to 11β-Hydrocortisone”, Applied Microbiology and Biotechnology, 2014, 98, 8879-8886.
26. Y. Duan, P. Yao, J. Ren, C. Han, Q. Li, J. Yuan, J. Feng, Q. Wu, D. Zhu, “Biocatalytic desymmetrization of 3-substituted glutaronitriles by nitrilases. A convenient chemoenzymatic access to optically active (S)-Pregabalin and (R)-Baclofen”, SCIENCE CHINA Chemistry, 2014, 57, 1164-1171.
27. H. Zhang, X. Gao, J. Ren, J. Feng, T. Zhang, Q. Wu, D. Zhu, “Enzymatic hydrogenation of diverse activated alkenes. Identification of two Bacillus old yellow enzymes with broad substrate profiles”, Journal of Molecular Catalysis B. Enzymatic, 2014, 105, 118-125.
28. 郭倩，张铁雨，冯进辉，王玉，陈曦，路福平，吴洽庆，朱敦明，“高效转化胆固醇菌株的筛选、鉴定及转化条件优化”，应用与环境生物学报，2014，20（1），101-106.
29. G. Li, J. Ren, P. Yao, Y. Duan, H. Zhang, Q. Wu, J. Feng, P. C. K. Lau, D. Zhu, “Deracemization of 2-Methyl-1, 2, 3, 4-tetrahydroquinoline Using Mutant Cyclohexylamine Oxidase Obtained by Iterative Saturation Mutagenesis”, ACS Catalysis, 2014, 4, 903-908 (Featured in the cover).
30. J. Jiang, X. Chen, J. Feng, Q. Wu, D. Zhu, “Substrate profile of a ω-transaminase from Burkholderia vietnamiensis and its potential for the production of optically pure amines and unnatural amino acids”, Journal of Molecular Catalysis B. Enzymatic, 2014, 100, 32-39.
31. B. Yang, Y. Wang , X. Chen , J. Feng , Q. Wu , D. Zhu, Y. Ma, “Biotransformations of steroids to testololactone by a multifunctional strain Penicillium simplicissimum WY134-2”, Tetrahedron, 2014, 70, 41-46.
32. W. Liu, Z. Li, C.-H. Huang, R.-T. Guo, L. Zhao, D. Zhang, X. Chen, Q. Wu, D. Zhu, “Structural and Mutational Studies on the Unusual Substrate Specificity of meso-Diaminopimelate Dehydrogenase from Symbiobacterium thermophilum”, ChemBioChem, 2014, 15, 217-222 (Featured in the inside cover).
33. G. Li, J. Ren, H. Iwaki, D. Zhang, Y. Hasegawa, Q. Wu, J. Feng, P. C. K. Lau, D. Zhu, “Substrate profiling of cyclohexylamine oxidase and its mutants reveals new biocatalytic potential in deracemization of racemic amines”, Applied Microbiology and Biotechnology, 2014, 98, 1681-1689.
34. X. Gao, F. Huang, J. Feng, X. Chen, H. Zhang, Z. Wang, Q. Wu, D. Zhu, “Engineering the meso-Diaminopimelate Dehydrogenase from Symbiobacterium thermophilum by Site-Saturation Mutagenesis for D-Phenylalanine Synthesis”, Applied and Environmental Microbiology, 2013, 79, 5078-5081.
35. 韩磊，陈曦，冯进辉，吴洽庆，朱敦明，来自Staphylococcus aureus N315的2-脱氧-d-核糖5-磷酸醛缩酶的工程菌构建、表达纯化与性质鉴定，生物加工过程，2013，11, 47-53。
36. 张海灵，高秀珍，陈曦，任杰，冯进辉，张同存，吴洽庆，朱敦明，米曲霉（Aspergillus oryzae） RIB40中烯酮/烯酯还原酶的异源表达及性质分析，生物加工过程，2013，11, 41-46。
37. 李倩，任杰，冯进辉，吴洽庆，朱敦明，重组固氮菌腈水合酶催化3-(4-氯苯基)戊二腈的选择性水解，生物加工过程， 2013，11, 23-28。
38. X. Liu, Q. Zhao, J. Ren, W. Dong, Q. Wu, D. Zhu, “N-terminal truncation of a maleate cis-trans isomerase from Rhodococcus sp. RHA1 results in a highly active enzyme for the biocatalytic production of fumaric acid”, Journal of Molecular Catalysis B. Enzymatic, 2013, 93, 44-50.
39. Z. Li, W. Liu, X. Chen, S. Jia, Q. Wu, D. Zhu, Y. Ma, “Highly enantioselective double reduction of phenylglyoxal to (R)-1-phenyl-1,2-ethanediol by one NADPH-dependent yeast carbonyl reductase with a broad substrate profile”, Tetrahedron, 2013, 69, 3561-3564.
40. G. Li, J. Ren, Q. Wu, J Feng, D. Zhu, Y. Ma, “Identification of a Marine NADPH-dependent Aldehyde Reductase for Chemoselective Reduction of Aldehydes”, Journal of Molecular Catalysis B. Enzymatic, 2013, 90, 17-22.
41. 周传华，陈曦，冯进辉，肖冬光，吴洽庆，朱敦明. 来源于Staphylococcus hominis的N-乙酰神经氨酸裂合酶的基因克隆及性质分析. 生物工程学报, 2013, 29(4), 已接受。
42. 田来强，刘卫东，陈曦，冯进辉，杨洪江，吴洽庆，朱敦明，马延和. 一种来源于毕赤酵母的高对映选择性羰基还原酶的性质及底物谱分析. 生物工程学报, 2013, 29(2), 169-179。
43. 徐美珍，任杰，龚劲松，董文玥，吴洽庆，许正宏，朱敦明. 生物催化3-(4-氯苯基)-戊二腈去对称性水解合成光学纯巴氯芬的关键前体. 生物工程学报, 2013, 29(1), 31-40。
44. 李哲, 刘卫东, 陈曦, 贾士儒，吴洽庆，朱敦明，马延和. 不对称还原大位阻二芳香基甲酮的羰基还原酶的基因克隆及性质分析. 生物工程学报, 2013, 29(1), 68−77。
45. Y. Wang, J. Li, Q. Wu, D. Zhu, “Microbial stereospecific reduction of 3-quinuclidinone with newly isolated Nocardia sp. and Rhodococcus erythropolis”, Journal of Molecular Catalysis B. Enzymatic, 2013, 88, 14-19.
46. H. Zhang, J. Ren, Y. Wang, C. Sheng, Q. Wu, A. Diao, D. Zhu, “Effective multi-step functional biotransformations of steroids by a newly isolated Fusarium oxysporum SC1301”, Tetrahedron, 2013, 69, 184-189.
47. R. Zhang, J. Ren, Y. Wang, Q. Wu, M. Wang, D. Zhu, “Isolation and characterization of a novel Rhodococcus strain with switchable carbonyl reductase and para-acetylphenol hydroxylase activities”, Journal of Industrial Microbiology and Biotechnology, 2013, 40,11-20.
48. X. Gao, X. Chen, W. Liu, J. Feng, Q. Wu, L. Hua, D. Zhu, “A Novel meso-Diaminopimelate Dehydrogenase from Symbiobacterium thermophilum. Overexpression, Characterization and Potential for D-amino Acid Synthesis”, Applied and Environmental Microbiology, 2012, 78, 8595-8600.
49. X. Chen, X. Gao, Q. Wu, D. Zhu, “Synthesis of optically active dihydrocarveol via stepwise or one-pot enzymatic reduction of (R)- and (S)-carvone”, Tetrahedron: Asymmetry, 2012, 23, 734-738.
50. J. Ren, W. Dong, B. Yu, Q. Wu, D. Zhu, “Synthesis of Optically Active α-Bromohydrins via Reduction of α-Bromoacetophenone Analogues Catalyzed by an Isolated Carbonyl Reductase”, Tetrahedron: Asymmetry, 2012, 23, 497-500.
51. X. Gao, J. Ren, Q. Wu, D. Zhu, “Biochemical characterization and substrate profiling of a new NADH- dependent enoate reductase from Lactobacillus casei”, Enzyme & Microbial Technology, 2012, 51, 26-34.
52. 生措，任杰，王敏，吴洽庆，朱敦明，“不对称还原a-氨基苯乙酮菌种的筛选及转化条件优化”，应用与环境生物学报，2012，18，281-286.
53. Y. Liu, T. Lv, J. Ren, M. Wang, Q. Wu, D. Zhu, “The catalytic promiscuity of a microbial 7α-hydroxysteroid dehydrogenase. Reduction of non-steroidal carbonyl compounds”, Steroids, 2011, 76, 1136-1140.
54. 刘祥涛，赵青，任杰，李正华，王昌禄，吴洽庆，朱敦明，“顺丁烯二酸异构酶工程菌发酵条件研究”，应用与环境生物学报，2011，17，558-562.
55. 吕彤，刘扬，赵青，任杰，王敏，吴洽庆，朱敦明，“烯酮还原酶基因的克隆与优化表达”，应用与环境生物学报，2011，17，87-90.
56. H. Li, Y. Yang, D. Zhu, L. Hua and K. Kantardjieff, “Highly Enantioselective Mutant Carbonyl Reductases Created via Structure-Based Site-Saturation Mutagenesis”, Journal of Organic Chemistry, 2010, 75, 7559-7564.
57. W. Wu, D. Zhu, L. Hua, “Site-saturation mutagenesis of formate dehydrogenase from Candida bodinii creating effective NADP+-dependent FDH enzymes”, Journal of Molecular Catalysis B: Enzymatic, 2009, 61, 157-161.
58. H. Li, D. Zhu, L. Hua and E. R. Biehl, “Enantioselective reduction of diaryl ketones catalyzed by a carbonyl reductase from Sporobolomyces salmonicolor and its mutant enzymes”, Advanced Synthesis & Catalysis, 2009, 351, 583-588.
59. H. Ankati, D. Zhu, Y. Yang, E. R. Biehl and L. Hua “Asymmetric Synthesis of Both Antipodes of b-Hydroxy Nitriles and b-Hydroxy Carboxylic Acids via Enzymatic Reduction or Sequential Reduction/Hydrolysis”, Journal of Organic Chemistry, 2009, 74, 1658-1662.
60. H. Li, D. Zhu, B. A. Hyatt, F. M. Malik, E. R. Biehl and L. Hua, “Cloning, protein sequence clarification and substrate specificity of a leucine dehydrogenase from Bacillus sphaericus ATCC4525”, Applied Biochemistry and Biotechnology, 2009, 158, 343-351.
61. D. Zhu, B. A. Hyatt, L. Hua, “Enzymatic Hydrogen Transfer Reduction of a-Chloro Aromatic Ketones Catalyzed by a Hyperthermophilic Alcohol Dehydrogenase”, Journal of Molecular Catalysis B: Enzymatic, 2009, 56, 272-276.
Students
已指导学生
韩磊 02 19586
陈杰 02 19294
李广悦 01 19294
高秀珍 01 19294
熊鑫 02 63220
迟婧 02 19294
姜进举 01 19294
丛培乾 02 63220
任杰 01 19294
现指导学生
刘娜 02 19294
鲁玮 01 19295
陈启佳 01 19295
李娟 02 63220