General
Xiaohong Fang Ph.D. Professor Physical Chemistry
Email：xfang@iccas.ac.cn
Tel：010-62650024
Fax：010-62650024
Research Areas
Ø Single molecule fluorescence imaging and single molecule force microscopy
Ø Novel molecular probe for real-time protein detection
Ø Nanobiosenor development
Education
1990: B.S., Ｗuhan University
1993: M.S., Beijing University
1996: Ph.D., Beijing University
1997-1998: Postdoc., University of Waterloo
Experience
Work Experience
1998-2001: Research Associate, University of Florida
2001.12: Professor, Institute of Chemistry, Chinese Academy of Sciences
Honors & Distinctions
The State Council Allowance, 2010
Award for Distinguished Contribution, All-China Federation of Returned Overseas Chinese, 2010
Distinguished Young Scientists Award, China Association for Instrumental Analysis, 2008
National Distinguished Scholars in the New Century, 2006
Distinguished Women Award, Chinese Academy of Sciences, 2005.
"Hundred Distinguished Young Scholars" Achievement Award, Chinese Academy of Sciences, 2005.
Distinguished Young Scientists Award , Chinese Chemical Society, 2003
Chinese National Science Fund for Distinguished Young Scholars, Chinese National Science Foundation, 2002.
"Hundred Distinguished Young Scholars", Chinese Academy of Sciences, 200
Award for Distinguished Contribution, All-China Federation of Returned Overseas Chinese, 2010
Distinguished Young Scientists Award, China Association for Instrumental Analysis, 2008
National Distinguished Scholars in the New Century, 2006
Distinguished Women Award, Chinese Academy of Sciences, 2005.
"Hundred Distinguished Young Scholars" Achievement Award, Chinese Academy of Sciences, 2005.
Distinguished Young Scientists Award , Chinese Chemical Society, 2003
Chinese National Science Fund for Distinguished Young Scholars, Chinese National Science Foundation, 2002.
"Hundred Distinguished Young Scholars", Chinese Academy of Sciences, 200
Publications
Selected publications （from over 110）:
Single-Molecule Detection of Proteins Using Aptamer-Functionalized Molecular Electronic Devices, Liu, S.; Zhang, X., Luo, W., Wang, Z.., Guo, X.*, Steigerwald, M., Fang, X.*, Angew. Chem. Int. Ed. 2011,DOI: 10.1002/anie.201006469
A single-molecule study of inhibition effect of Naringenin on transforming growth factor-b ligand-receptor binding, Yang, Y., Xu, Y., Xia, T., Chen, F., Zhang, F., Liang, W., Fang, X.* , Chem. Commun. 2011, 47, 2982-2984
A Water-Soluble C60-Porphyrin Compound for Highly Efficient DNA Photocleavage, Zhou, C.*, Liu, Q., Xu, W., Wang, C. and Fang, X.*, Chem. Commun., 2011, 47, 2982-2984
Elasticity of Cardiac Cells on the Polymer Substrates with Different Stiffness: An Atomic Force Microscopy Study, Shi, X., Qin, L., Zhang, X., He, K., Xiong, C., Fang, J., Fang, X.*, Zhang, Y.*, Phys. Chem. Chem. Phys., 2011, 13 (16), 7540-5.
Single-Molecule Imaging Revealed Enhanced Dimerization of Transforming Growth Factor β Type Ⅱ Receptors in Hypertrophic Cardiomyocytes” He, K., Fu, Y., Zhang, W., Yuan, J., Li, Z., Lv, Z., Zhang, Y*, Fang, X.* Biochem. Biophy. Res. Commun., 2011,407，313-317.
Single-molecule fluorescence imaging of membrane-bound proteins for studies of cell signaling transduction，Yang, Y., Xia, T., Zhang, W., Fang, X.* Chinese Science Bulletin, 2011, 56(11), 1063-67.，
TGF-β signalling is mediated by two autonomously functioning TβRI:TβRII pairs. Huang T, David L, Mendoza V, Yang Y, Villarreal M, De K, Sun L, Fang X, López-Casillas F, Wrana JL, Hinck AP，EMBO J. 2011，30(7): 1263-76.
Aptamers Generated from Cell-SELEX for Molecular Medicine：A Chemical Biology Approach, Fang, X.* and Tan, W*, Acc. Chem. Res., 2010, 43, 48-57.
Intercellular transportation of quantum dots mediated by membrane nanotubes, He, K., Luo, W., Zhang, Y., Liu, F., Liu, D., Xu, L., Qin, L., Xiaong, C., Lu, Z., Fang, X.*, Zhang, Y.*, ACS Nano, 2010, 4，3015-3022。
Study of the inhibitory effect of water-soluble fullerenes on plant growth at the cellular level. Liu, Q., Zhao, Y., Wan, Y., Zhang, J., Zhang, X., Wang, C., Fang, X. * and Lin., J.* , ACS Nano, 2010, 4，5743-5748.
Monomeric Type I and Type III Transforming Growth Factor-β Receptors and Their Dimerization, Zhang, Z., Yuan, J., Yang, Y., Xu, L., Wang, Q., Fang, X.*, Chen, Y.*, Cell Res., 2010. 20，1216-1223.
Single-Molecule Imaging Reveals Transforming Growth Factor-b-induced Type II Receptor Dimerization. Zhang, W., Jiang, Y., Wang , Q., Ma, X., Xiao, Z., Zuo, W., Fang, X.*, Chen, Y.* Proc. Natl. Acad. Sci. USA, 2009, 106, 15679-15683.
Carbon Nanotubes as Molecular Transporters for Walled Plant Cells, Liu, Q., Bo. C., Wang, Q., Shi, X., Xiao, Z., Lin., J., and Fang, X. *, Nano Letters, 2009, 9: 1007-1010.
Study of inhibition effect of herceptin on interaction between heregulin and ErbB receptors HER3/HER2 by single-molecule force spectroscopy, Shi, X., Xu, L., Yu., J., and Fang, X. *, Exp. Cell. Res. 2009.
Molecular engineering of DNA: molecular beacons.K. Wang*, Z. Tang, C. Yang, Y. M. Kim, X. Fang, W. Li, Y. Wu, C. Medley, Z. Cao, J. Li, P. Colon, H. Lin, W. Tan*. Angew. Chem. Int. Ed., 2009, 48, 856-870.
Hydrodynamically Focused Stream as a Dynamic Template for Site-Specific Electrochemical Micropatterning of Conducting Polymers, Hou, S., Wang, S., Zeta, T., Yu, F., Nicole, Q., Zhu, M., Clifton, K,. Shen, F., Fang, X. *, Tseng, H.*, Angew. Chem. Int. Ed. 2008, 47,1072-1075
Single-Molecule Study of Lateral Mobility of Epidermal Growth Factor Receptor 2/HER2 on Activation，Z. Xiao, X. Ma, Y. Jiang, Z. Zhao, B. Lai, J. Liao, J. Yue and X. Fang*，J. Phy. Chem. B, 2008, 112: 4140-4145.
Detection of trace Hg2+ via induced circular dichroism of DNA wrapped around single-walled carbon nanotubes. X. Gao, G. Xing, Y. Yang, X. Shi, R. Liu, W. Chu, L. Jing, F. Zhao, C. Ye, H. Yuan, X. Fang, C. Wang, and Y. Zhao. J. Am. Chem. Soc., 2008, 130: 9190–9191.
TINY, a dehydration-responsive element(DRE)-binding protein-like transcription factor connecting the DRE-and ethylene-responsive element-mediated signaling patheways in Arabidopsis. S. Sun, J. Yu, F. Chen, T. Zhao, X. Fang, Y. Li, and S. Sui. J. Biol. Chem. 2008, 283: 6261-6271.
Single-Molecule Study of EGFR Mobility Implicates the its Association with Caveolae/Clathrin After Activation，Z. Xiao, W. Zhang, Y. Yang, L. Xu, X. Fang* Biochem Biophys Res Commun. 2008, 369: 730-734.
Conjugation of a Water-Soluble Gadolinium Endohedral Fulleride with an Antibody as a Magnetic Resonance Imaging Contrast Agent， Shu，C., Ma, X., Zhang, J., Corwin, F., Sim, J., Zhang, E., Dorn, H., Gibson, H., Fatouros, P., Wang, C.*, Fang, X. * Bioconjugate Chem., 2008, 19: 651-655.
Cell-specific Internalization study of an Aptamer From Whole Cell Selection, Xiao, Z., Shangguan, D., Cao, Z., Fang, X. and Tan, W. *, Chem. Eur. J., 2008, 14, 1679-1755.
Single Molecule Force Spectroscopy Study of Interaction between Transforming Growth Factor B and its Receptor in Living Cells，Yu., J., Wang Q., Shi X. , Ma, X., Yang H., Chen, Y., Fang, X*, J. J. Phy. Chem. B, 2007, 111(48):13619-13625
Lateral Diffusion of TGF-b Type Receptor Studied by Single Molecule Imaging, Ma, X., Wang,Q., Jiang, Y., Xiao, Z., Fang, X. *, Chen, Y*., Biochem Biophys Res Commun. 2007, 292, 31-40.
Interaction between Single Molecules of Mac-1 and ICAM-1 in Living Cells: An Atomic Force Microscopy Study, Yang, H., Yu, J. Fu, G., Shi, X., Xiao, L., Chen, Y., Fang, X*., Chen, H*., Exp. Cell. Res. 2007, 331, 3497-3504
Energy Landscape of Aptamer/Protein Complex Studied by Single Molecular Force Spectroscopy, Yu., J., Jiang, Y., Ma, X., Fang, X. * Chem. Asia. J., 2007, 2, 284-289.
Fluorescence study of DNA binding and bending by EcoRI DNA methyltransferase, Ma，B., Wang, J., Fang, X. *, J. Phy. Chem. B, 2006,110, 19647-19651.
Single Molecule Study of Binding Force Between Transcription Factor TINY and Its DNA Responsive Element, Yu, J., Sun, S., Jiang, Y., Ma, X., Chen, F., Zhang, G., Fang, X*., Polymer, 2006, 47, 2533-2538.
Detection of Oncoprotein Platelet-Derived Growth Factor Using Fluorescent Signaling Complex of Aptamer and TOTO，Zhou, C. Wang, J., Jiang, Y., Hou, S., Fang, X. *, Anal. Bioanal. Chem., 2006, 384, 1175-1180.
Aptamer Based ATP Assay Using a Luminescent Light Switching Complex, Wang, J., Jiang, Y., Zhou, C., Fang, X *. Anal. Chem., 2005, 77,3542-3546. (5.6)
Study of Fibrinogen Adsorption on Self-Assembled Monolayers on Au(111) by Atomic Force Microscopy，Lin ,Y., Wang, J., Wan L. , Fang X. *, Ultramicroscopy, 2005, 105, 129-136.
Study of the Single Molecular Interaction of the Transcription Factor ZmDREB1A with its DNA Responsive Element by Atomic Force Microscopy. Jiang, Y. X., Feng, Q., Fang, X*, Bai, C *. Nucl.Acid.Res. 2004, 32(12): e101.
Signaling Aptamer/Protein Binding by a Molecular Light-Switch Complex. Jiang, Y., Fang, X *, Bai, C *. Anal. Chem. 2004, 76, 5230-5235.
Delaying Photobleaching and Recovering Luminescence of a DNA Molecular Light Switch in DNA Analysis, Ling, L., Jiang, Y., Wang, C., Fang, X.*, Wan, L. He, Z., Bai, C.*, Chen.,D.*, Anal. Biochem. 2004, 329, 334-336.
Specific Aptamer - Protein Interaction Studied by Atomic Force Microscopy. Jiang, Y., Zhu, C. F., Ling, L., Wan, L., Fang, X *, Bai, C.*, Anal. Chem., 2003, 75(9): 2112-2116.
Study of Citrate Adsorbed on the Au(111) Surface by Scanning Probe Microscopy. Lin, Y., Pan, G.., Su, G. ., Fang, X.*, Wan, L.*., Bai, C.*. Langmuir 2003, 19, 10000-1003
Single-Molecule Detection of Proteins Using Aptamer-Functionalized Molecular Electronic Devices, Liu, S.; Zhang, X., Luo, W., Wang, Z.., Guo, X.*, Steigerwald, M., Fang, X.*, Angew. Chem. Int. Ed. 2011,DOI: 10.1002/anie.201006469
A single-molecule study of inhibition effect of Naringenin on transforming growth factor-b ligand-receptor binding, Yang, Y., Xu, Y., Xia, T., Chen, F., Zhang, F., Liang, W., Fang, X.* , Chem. Commun. 2011, 47, 2982-2984
A Water-Soluble C60-Porphyrin Compound for Highly Efficient DNA Photocleavage, Zhou, C.*, Liu, Q., Xu, W., Wang, C. and Fang, X.*, Chem. Commun., 2011, 47, 2982-2984
Elasticity of Cardiac Cells on the Polymer Substrates with Different Stiffness: An Atomic Force Microscopy Study, Shi, X., Qin, L., Zhang, X., He, K., Xiong, C., Fang, J., Fang, X.*, Zhang, Y.*, Phys. Chem. Chem. Phys., 2011, 13 (16), 7540-5.
Single-Molecule Imaging Revealed Enhanced Dimerization of Transforming Growth Factor β Type Ⅱ Receptors in Hypertrophic Cardiomyocytes” He, K., Fu, Y., Zhang, W., Yuan, J., Li, Z., Lv, Z., Zhang, Y*, Fang, X.* Biochem. Biophy. Res. Commun., 2011,407，313-317.
Single-molecule fluorescence imaging of membrane-bound proteins for studies of cell signaling transduction，Yang, Y., Xia, T., Zhang, W., Fang, X.* Chinese Science Bulletin, 2011, 56(11), 1063-67.，
TGF-β signalling is mediated by two autonomously functioning TβRI:TβRII pairs. Huang T, David L, Mendoza V, Yang Y, Villarreal M, De K, Sun L, Fang X, López-Casillas F, Wrana JL, Hinck AP，EMBO J. 2011，30(7): 1263-76.
Aptamers Generated from Cell-SELEX for Molecular Medicine：A Chemical Biology Approach, Fang, X.* and Tan, W*, Acc. Chem. Res., 2010, 43, 48-57.
Intercellular transportation of quantum dots mediated by membrane nanotubes, He, K., Luo, W., Zhang, Y., Liu, F., Liu, D., Xu, L., Qin, L., Xiaong, C., Lu, Z., Fang, X.*, Zhang, Y.*, ACS Nano, 2010, 4，3015-3022。
Study of the inhibitory effect of water-soluble fullerenes on plant growth at the cellular level. Liu, Q., Zhao, Y., Wan, Y., Zhang, J., Zhang, X., Wang, C., Fang, X. * and Lin., J.* , ACS Nano, 2010, 4，5743-5748.
Monomeric Type I and Type III Transforming Growth Factor-β Receptors and Their Dimerization, Zhang, Z., Yuan, J., Yang, Y., Xu, L., Wang, Q., Fang, X.*, Chen, Y.*, Cell Res., 2010. 20，1216-1223.
Single-Molecule Imaging Reveals Transforming Growth Factor-b-induced Type II Receptor Dimerization. Zhang, W., Jiang, Y., Wang , Q., Ma, X., Xiao, Z., Zuo, W., Fang, X.*, Chen, Y.* Proc. Natl. Acad. Sci. USA, 2009, 106, 15679-15683.
Carbon Nanotubes as Molecular Transporters for Walled Plant Cells, Liu, Q., Bo. C., Wang, Q., Shi, X., Xiao, Z., Lin., J., and Fang, X. *, Nano Letters, 2009, 9: 1007-1010.
Study of inhibition effect of herceptin on interaction between heregulin and ErbB receptors HER3/HER2 by single-molecule force spectroscopy, Shi, X., Xu, L., Yu., J., and Fang, X. *, Exp. Cell. Res. 2009.
Molecular engineering of DNA: molecular beacons.K. Wang*, Z. Tang, C. Yang, Y. M. Kim, X. Fang, W. Li, Y. Wu, C. Medley, Z. Cao, J. Li, P. Colon, H. Lin, W. Tan*. Angew. Chem. Int. Ed., 2009, 48, 856-870.
Hydrodynamically Focused Stream as a Dynamic Template for Site-Specific Electrochemical Micropatterning of Conducting Polymers, Hou, S., Wang, S., Zeta, T., Yu, F., Nicole, Q., Zhu, M., Clifton, K,. Shen, F., Fang, X. *, Tseng, H.*, Angew. Chem. Int. Ed. 2008, 47,1072-1075
Single-Molecule Study of Lateral Mobility of Epidermal Growth Factor Receptor 2/HER2 on Activation，Z. Xiao, X. Ma, Y. Jiang, Z. Zhao, B. Lai, J. Liao, J. Yue and X. Fang*，J. Phy. Chem. B, 2008, 112: 4140-4145.
Detection of trace Hg2+ via induced circular dichroism of DNA wrapped around single-walled carbon nanotubes. X. Gao, G. Xing, Y. Yang, X. Shi, R. Liu, W. Chu, L. Jing, F. Zhao, C. Ye, H. Yuan, X. Fang, C. Wang, and Y. Zhao. J. Am. Chem. Soc., 2008, 130: 9190–9191.
TINY, a dehydration-responsive element(DRE)-binding protein-like transcription factor connecting the DRE-and ethylene-responsive element-mediated signaling patheways in Arabidopsis. S. Sun, J. Yu, F. Chen, T. Zhao, X. Fang, Y. Li, and S. Sui. J. Biol. Chem. 2008, 283: 6261-6271.
Single-Molecule Study of EGFR Mobility Implicates the its Association with Caveolae/Clathrin After Activation，Z. Xiao, W. Zhang, Y. Yang, L. Xu, X. Fang* Biochem Biophys Res Commun. 2008, 369: 730-734.
Conjugation of a Water-Soluble Gadolinium Endohedral Fulleride with an Antibody as a Magnetic Resonance Imaging Contrast Agent， Shu，C., Ma, X., Zhang, J., Corwin, F., Sim, J., Zhang, E., Dorn, H., Gibson, H., Fatouros, P., Wang, C.*, Fang, X. * Bioconjugate Chem., 2008, 19: 651-655.
Cell-specific Internalization study of an Aptamer From Whole Cell Selection, Xiao, Z., Shangguan, D., Cao, Z., Fang, X. and Tan, W. *, Chem. Eur. J., 2008, 14, 1679-1755.
Single Molecule Force Spectroscopy Study of Interaction between Transforming Growth Factor B and its Receptor in Living Cells，Yu., J., Wang Q., Shi X. , Ma, X., Yang H., Chen, Y., Fang, X*, J. J. Phy. Chem. B, 2007, 111(48):13619-13625
Lateral Diffusion of TGF-b Type Receptor Studied by Single Molecule Imaging, Ma, X., Wang,Q., Jiang, Y., Xiao, Z., Fang, X. *, Chen, Y*., Biochem Biophys Res Commun. 2007, 292, 31-40.
Interaction between Single Molecules of Mac-1 and ICAM-1 in Living Cells: An Atomic Force Microscopy Study, Yang, H., Yu, J. Fu, G., Shi, X., Xiao, L., Chen, Y., Fang, X*., Chen, H*., Exp. Cell. Res. 2007, 331, 3497-3504
Energy Landscape of Aptamer/Protein Complex Studied by Single Molecular Force Spectroscopy, Yu., J., Jiang, Y., Ma, X., Fang, X. * Chem. Asia. J., 2007, 2, 284-289.
Fluorescence study of DNA binding and bending by EcoRI DNA methyltransferase, Ma，B., Wang, J., Fang, X. *, J. Phy. Chem. B, 2006,110, 19647-19651.
Single Molecule Study of Binding Force Between Transcription Factor TINY and Its DNA Responsive Element, Yu, J., Sun, S., Jiang, Y., Ma, X., Chen, F., Zhang, G., Fang, X*., Polymer, 2006, 47, 2533-2538.
Detection of Oncoprotein Platelet-Derived Growth Factor Using Fluorescent Signaling Complex of Aptamer and TOTO，Zhou, C. Wang, J., Jiang, Y., Hou, S., Fang, X. *, Anal. Bioanal. Chem., 2006, 384, 1175-1180.
Aptamer Based ATP Assay Using a Luminescent Light Switching Complex, Wang, J., Jiang, Y., Zhou, C., Fang, X *. Anal. Chem., 2005, 77,3542-3546. (5.6)
Study of Fibrinogen Adsorption on Self-Assembled Monolayers on Au(111) by Atomic Force Microscopy，Lin ,Y., Wang, J., Wan L. , Fang X. *, Ultramicroscopy, 2005, 105, 129-136.
Study of the Single Molecular Interaction of the Transcription Factor ZmDREB1A with its DNA Responsive Element by Atomic Force Microscopy. Jiang, Y. X., Feng, Q., Fang, X*, Bai, C *. Nucl.Acid.Res. 2004, 32(12): e101.
Signaling Aptamer/Protein Binding by a Molecular Light-Switch Complex. Jiang, Y., Fang, X *, Bai, C *. Anal. Chem. 2004, 76, 5230-5235.
Delaying Photobleaching and Recovering Luminescence of a DNA Molecular Light Switch in DNA Analysis, Ling, L., Jiang, Y., Wang, C., Fang, X.*, Wan, L. He, Z., Bai, C.*, Chen.,D.*, Anal. Biochem. 2004, 329, 334-336.
Specific Aptamer - Protein Interaction Studied by Atomic Force Microscopy. Jiang, Y., Zhu, C. F., Ling, L., Wan, L., Fang, X *, Bai, C.*, Anal. Chem., 2003, 75(9): 2112-2116.
Study of Citrate Adsorbed on the Au(111) Surface by Scanning Probe Microscopy. Lin, Y., Pan, G.., Su, G. ., Fang, X.*, Wan, L.*., Bai, C.*. Langmuir 2003, 19, 10000-1003