1. 母乳寡糖和糖蛋白糖链的纯化制备和结构分析
    以母乳为研究对象，发展母乳中游离寡糖、糖脂、糖蛋白糖链的纯化制备方法，获得单体化合物，结合质谱、核磁共振波谱等方法对结构进行​鉴定和解析。同时，建立母乳寡糖分析方法，对不同血型、年龄、哺乳时期母乳进行分析，解析其寡糖差别。
2. 构建生物源寡糖库
    以生物来源天然寡糖为研究对象，纯化寡糖单体，构建寡糖的标准品库。
3. 寡糖功能研究
    通过寡糖芯片的方法，研究寡糖与病毒、致病菌的作用。

   

070302-分析化学

寡糖分离分析，寡糖库构建，母乳寡糖

2006-09--2011-01 中国科学院大连化学物理研究所 理学博士
2003-09--2006-07 沈阳药科大学 理学硕士
1999-09--2003-07 沈阳药科大学 工学学士

   

2013-08--今 中国科学院大连化学物理研究所 副研究员
2011-02--2013-07 中国科学院大连化学物理研究所 助理研究员

   

[1] Li, Jiaqi, Jiang, Maorong, Zhou, JiaoRui, Ding, Junjie, Guo, Zhimou, Li, Ming, Ding, Fei, Chai, Wengang, Yan, Jingyu, Liang, Xinmiao. Characterization of rat and mouse acidic milk oligosaccharides based on hydrophilic interaction chromatography coupled with electrospray tandem mass spectrometry. CARBOHYDRATE POLYMERS[J]. 2021, 259: http://dx.doi.org/10.1016/j.carbpol.2021.117734.
[2] 游星, 郭进, 张洪涛, 闫竞宇, CHAI, Wengang, 周文, 刘栗彤, 黎玉, 吴剑荣, 詹晓北. 基于混菌耦合发酵策略合成3′-唾液酸乳糖. 食品与发酵工业. 2021, 47(17): 8-14, [3] Jiang Junpeng, Yang Miao, Cao Xupeng, Wan Huihui, Liu Shuqin, Zhong Shijun, Yan Jingyu, Xue Song. A Novel Material for Selective Separation of Monogalactosyldiacylglycerols from Microalgae. JOURNAL OF OCEAN UNIVERSITY OF CHINA[J]. 2021, 20(1): 221-227, http://lib.cqvip.com/Qikan/Article/Detail?id=7103970235.
[4] Cuiyan Cao, Long Yu, Jingyu Yan, Dongmei Fu, Jingli Yuan, Xinmiao Liang. Purification of natural neutral N-glycans by using two-dimensional hydrophilic interaction liquid chromatography × porous graphitized carbon chromatography for glycan-microarray assay. Talanta. 2021, 221: http://dx.doi.org/10.1016/j.talanta.2020.121382.
[5] Cao, Cuiyan, Yu, Long, Yan, Jingyu, Fu, Dongmei, Yuan, Jingli, Liang, Xinmiao. Purification of natural neutral N-glycans by using two-dimensional hydrophilic interaction liquid chromatography x porous graphitized carbon chromatography for glycan-microarray assay. TALANTA[J]. 2021, 221: https://www.webofscience.com/wos/woscc/full-record/WOS:000579910600007.
[6] 金高娃, 宋春颖, 闫竞宇, 刘艳芳, 沈爱金, 郭志谋, 梁鑫淼. 超高效液相色谱-质谱法同时测定舒血宁注射剂中20种黄酮醇苷与萜类内酯成分. 分析测试学报[J]. 2020, 39(1): 115-121, http://lib.cqvip.com/Qikan/Article/Detail?id=7101155755.
[7] Huang, Chuncui, Sun, Shiwei, Yan, Jingyu, Wang, Hui, Zhou, Jinyu, Gao, Huanyu, Xie, Wenchun, Li, Yan, Chai, Wengang. Identification of carbohydrate peripheral epitopes important for recognition by positive-ion MALDI multistage mass spectrometry. CARBOHYDRATE POLYMERS[J]. 2020, 229: http://dx.doi.org/10.1016/j.carbpol.2019.115528.
[8] Wei Si, Yanfang Liu, Yuansheng Xiao, Zhimou Guo, Gaowa Jin, Jingyu Yan, Aijin Shen, Han Zhou, Fan Yang, Xinmiao Liang. An offline two-dimensional supercritical fluid chromatography × reversed phase liquid chromatography tandem quadrupole time-of-flight mass spectrometry system for comprehensive gangliosides profiling in swine brain extract. Talanta. 2020, 208: http://dx.doi.org/10.1016/j.talanta.2019.120366.
[9] Si, Wei, Liu, Yanfang, Xiao, Yuansheng, Guo, Zhimou, Jin, Gaowa, Yan, Jingyu, Shen, Aijin, Zhou, Han, Yang, Fan, Liang, Xinmiao. An offline two-dimensional supercritical fluid chromatography x reversed phase liquid chromatography tandem quadrupole time-of-flight mass spectrometry system for comprehensive gangliosides profiling in swine brain extract. TALANTA[J]. 2020, 208: https://www.webofscience.com/wos/woscc/full-record/WOS:000503314200019.
[10] Song Chunying, Zhang Huarong, Guo Zhimou, Yan Jingyu, Jin Gaowa, Liang Xinmiao. Determination of five protopanaxadiol ginsenosides in ginseng by solid-phase extraction-ultra performance liquid chromatography. CHINESE JOURNAL OF CHROMATOGRAPHY[J]. 2020, 38(5): 547-553, [11] 宋春颖, 张华蓉, 郭志谋, 闫竞宇, 金高娃, 梁鑫淼. 固相萃取-超高效液相色谱法测定人参中5种原人参二醇型人参皂苷的含量. 色谱[J]. 2020, 38(5): 547-553, http://lib.cqvip.com/Qikan/Article/Detail?id=7101380673.
[12] Huang, Chuncui, Yan, Jingyu, Zhan, Lingpeng, Zhao, Min, Zhou, Jinyu, Gao, Huanyu, Xie, Wenchun, Li, Yan, Chai, Wengang. Linkage and sequence analysis of neutral oligosaccharides by negative-ion MALDI tandem mass spectrometry with laser-induced dissociation. ANALYTICA CHIMICA ACTA[J]. 2019, 1071: 25-35, http://cas-ir.dicp.ac.cn/handle/321008/171871.
[13] Gao, Tian, Yan, Jingyu, Liu, ChangCheng, Palma, Angelina S, Guo, Zhimou, Xiao, Min, Chen, Xi, Liang, Xinmiao, Chai, Wengang, Cao, Hongzhi. Chemoenzymatic Synthesis of O-Mannose Glycans Containing Sulfated or Nonsulfated HNK-1 Epitope. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY[J]. 2019, 141(49): 19351-19359, https://www.webofscience.com/wos/woscc/full-record/WOS:000502687800024.
[14] Yan, Jingyu, Ding, Junjie, Jin, Gaowa, Duan, Zhaojun, Yang, Fan, Li, Dandi, Zhou, Han, Li, Ming, Guo, Zhimou, Chai, Wengang, Liang, Xinmiao. Profiling of Human Milk Oligosaccharides for Lewis Epitopes and Secretor Status by Electrostatic Repulsion Hydrophilic Interaction Chromatography Coupled with Negative-Ion Electrospray Tandem Mass Spectrometry. ANALYTICAL CHEMISTRY[J]. 2019, 91(13): 8199-8206, [15] 周娇锐, 白亚强, 刘曼, 郝思嘉, 李健新, 胡雨奇, 张利龙, 许尧, 袁杰力, 闫竞宇, 李文哲, 李明. 东北地区分泌型和非分泌型母亲哺乳期HMO差异及其对子代肠道菌群的影响. 中国微生态学杂志[J]. 2019, 380-383,388, http://lib.cqvip.com/Qikan/Article/Detail?id=90718783504849574852484850.
[16] Licitra, Rosario, Li, Jiaqi, Liang, Xinmiao, Altomonte, Iolanda, Salari, Federica, Yan, Jingyu, Martini, Mina. Profile and content of sialylated oligosaccharides in donkey milk at early lactation. LWT-FOOD SCIENCE AND TECHNOLOGY[J]. 2019, 115: 108437-, http://dx.doi.org/10.1016/j.lwt.2019.108437.
[17] 张华林, 郭志谋, 王联芝, 金高娃, 邹丽红, 吕园园, 马明辉, 闫竞宇, 段正超, 梁鑫淼. 基于电喷雾-四极杆-飞行时间质谱的神经节苷脂的结构解析. 分析化学[J]. 2019, 47(6): 925-932, http://lib.cqvip.com/Qikan/Article/Detail?id=7002100503.
[18] Rosario Licitra, Jiaqi Li, Xinmiao Liang, Iolanda Altomonte, Federica Salari, Jingyu Yan, Mina Martini. Profile and content of sialylated oligosaccharides in donkey milk at early lactation. LWT. 2019, 115: 108437-, http://dx.doi.org/10.1016/j.lwt.2019.108437.
[19] Yan, Jingyu, Ding, Junjie, Jin, Gaowa, Yu, Dongping, Yu, Long, Lone, Zhen, Guo, Zhimou, Chai, Wengang, Liang, Xinmiao. Profiling of Sialylated Oligosaccharides in Mammalian Milk Using Online Solid Phase Extraction-Hydrophilic Interaction Chromatography Coupled with Negative-Ion Electrospray Mass Spectrometry. ANALYTICAL CHEMISTRY[J]. 2018, 90(5): 3174-3182, http://cas-ir.dicp.ac.cn/handle/321008/168864.
[20] 张华蓉, 郭志谋, 于伟, 闫竞宇, 金高娃, 王联芝. 基于表面静电排斥/反相混合模式色谱的黄连生物碱分析方法. 色谱[J]. 2018, 36(10): 1053-1060, http://lib.cqvip.com/Qikan/Article/Detail?id=676296585.
[21] 闫竞宇, 丁俊杰, 金高娃, 郭志谋, 梁鑫淼. 人乳寡糖的分离分析. 生物技术进展. 2018, 8(6): 469-476, http://lib.cqvip.com/Qikan/Article/Detail?id=7000958512.
[22] Zhou, Weijia, Guo, Zhimou, Yu, Long, Zhou, Han, Shen, Aijin, Jin, Yan, Jin, Gaowa, Yan, Jingyu, Yang, Fan, Liu, Yanming, Wang, ChaoRan, Feng, JiaTao, Liu, Yanfang, Liang, Xinmiao. On-line comprehensive two-dimensional liquid chromatography tandem mass spectrometry for the analysis of Curcuma kwangsiensis. TALANTA[J]. 2018, 186: 73-79, http://dx.doi.org/10.1016/j.talanta.2018.04.014.
[23] Sun, Xitong, Yu, Wei, Yan, Jingyu, Li, Jiaqi, Jin, Gaowa, Feng, Jiatao, Guo, Zhimou, Liang, Xinmiao. Mesoporous silica-carbon composites fabricated by a universal strategy of hydrothermal carbonization: controllable synthesis and applications. RSC ADVANCES[J]. 2018, 8(48): 27207-27215, http://cas-ir.dicp.ac.cn/handle/321008/166950.
[24] Dong, Xuefang, Qin, Hongqiang, Mao, Jiawei, Yu, Dongping, Li, Xiuling, Shen, Aijin, Yan, Jingyu, Yu, Long, Guo, Zhimou, Ye, Mingliang, Zou, Hanfa, Liang, Xinmiao. In-Depth Analysis of Glycoprotein Sialylation in Serum Using a Dual-Functional Material with Superior Hydrophilicity and Switchable Surface Charge. ANALYTICAL CHEMISTRY[J]. 2017, 89(7): 3966-3972, http://cas-ir.dicp.ac.cn/handle/321008/169302.
[25] Yan, Jingyu, Ding, Junjie, Liang, Xinmiao. Chromatographic methods for the analysis of oligosaccharides in human milk. ANALYTICAL METHODSnull. 2017, 9(7): 1071-1077, http://cas-ir.dicp.ac.cn/handle/321008/169432.
[26] 杨铎, 俞冬萍, 董雪芳, 沈爱金, 金高娃, 郭志谋, 闫竞宇, 刘名扬, 梁鑫淼. 基于巯基-烯烃点击化学的聚丙烯酰胺型亲水作用色谱固定相的制备. Chinese Journal of Analytical Chemistry分析化学[J]. 2016, 43(10): 1439-, http://cas-ir.dicp.ac.cn/handle/321008/148016.
[27] Yu, Dongping, Guo, Zhimou, Shen, Aijin, Yan, Jingyu, Dong, Xuefang, Jin, Gaowa, Long, Zhen, Liang, Lina, Liang, Xinmiao. Synthesis and evaluation of sulfobetaine zwitterionic polymer bonded stationary phase. TALANTA[J]. 2016, 161: 860-866, http://cas-ir.dicp.ac.cn/handle/321008/169842.
[28] 闫竞宇, 郭志谋, 丁俊杰, 沈爱金, 王纪霞, 金高娃, 梁鑫淼. 万古霉素及其杂质的亲水作用色谱分析. Chinese Journal of Chromatography色谱[J]. 2015, 33(9): 951-, http://cas-ir.dicp.ac.cn/handle/321008/148015.
[29] 石先哲, 李攻科, 梁振, 郭志谋, 叶明亮, 闫竞宇, 许国旺. 色谱研究最新进展. 化学通报[J]. 2015, 77(7): 720-, http://cas-ir.dicp.ac.cn/handle/321008/143805.
[30] 闫竞宇, 郭志谋, 丁俊杰, 沈爱金, 王纪霞, 金高娃, 梁鑫淼. 万古霉素及其杂质的亲水作用色谱分析. 色谱[J]. 2015, 33(9): 951-956, http://lib.cqvip.com/Qikan/Article/Detail?id=665964914.
[31] Wei, Jie, Shen, Aijin, Wan, Huihui, Yan, Jingyu, Yang, Bingcheng, Guo, Zhimou, Zhang, Feifang, Liang, Xinmiao. Highly selective separation of aminoglycoside antibiotics on a zwitterionic Click TE-Cys column. JOURNAL OF SEPARATION SCIENCE[J]. 2014, 37(14): 1781-1787, http://www.irgrid.ac.cn/handle/1471x/858778.
[32] 金高娃, 丁俊杰, 陈雪, 闫竞宇, 郭志谋, 张秀莉, 梁鑫淼. 反相／亲水色谱法分析糖苷类化合物. 分析测试学报. 2014, 33(2): 133-137, http://lib.cqvip.com/Qikan/Article/Detail?id=48630437.
[33] Li, Xiaolong, Liu, Yanfang, Shen, Aijin, Wang, Chaoran, Yan, Jingyu, Zhao, Weijie, Liang, Xinmiao. Efficient purification of active bufadienolides by a class separation method based on hydrophilic solid-phase extraction and reversed-phase high performance liquid chromatography. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS[J]. 2014, 97: 54-64, http://dx.doi.org/10.1016/j.jpba.2014.04.015.
[34] Wang, Jixia, Kong, Song, Yan, Jingyu, Jin, Gaowa, Guo, Zhimou, Shen, Aijin, Xu, Junyan, Zhang, Xiuli, Zou, Lijuan, Liang, Xinmiao. Hydrophilic interaction liquid chromatography-solid phase extraction directly combined with protein precipitation for the determination of triptorelin in plasma. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES[J]. 2014, 960: 214-221, http://dx.doi.org/10.1016/j.jchromb.2014.04.036.
[35] Xu, Junyan, Zhang, Xiuli, Guo, Zhimou, Yan, Jingyu, Yu, Long, Li, Xiuling, Xue, Xingya, Liang, Xinmiao. Orthogonal separation and identification of long-chain peptides from scorpion Buthus martensi Karsch venom by using two-dimensional mixed-mode reversed phase-reversed phase chromatography coupled to tandem mass spectrometry. ANALYST[J]. 2013, 138(6): 1835-1843, http://www.irgrid.ac.cn/handle/1471x/721017.
[36] Zhao, Yanyan, Li, Xiuling, Yan, Jingyu, Guo, Zhimou, Liang, Xinmiao. Phosphopeptide enrichment and fractionation by using Click OEG-CD matrix. ANALYTICAL METHODS[J]. 2012, 4(5): 1244-1251, http://www.irgrid.ac.cn/handle/1471x/721148.
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[38] Wan, Huihui, Yan, Jingyu, Yu, Long, Sheng, Qianying, Zhang, Xiuli, Xue, Xingya, Li, Xiuling, Liang, Xinmiao. Zirconia layer coated mesoporous silica microspheres as HILIC SPE materials for selective glycopeptide enrichment. ANALYST[J]. 2011, 136(21): 4422-4430, http://www.irgrid.ac.cn/handle/1471x/456724.
[39] Yan, Jingyu, Li, Xiuling, Yu, Long, Jin, Yu, Zhang, Xiuli, Xue, Xingya, Ke, Yanxiong, Liang, Xinmiao. Selective enrichment of glycopeptides/phosphopeptides using porous titania microspheres. CHEMICAL COMMUNICATIONS[J]. 2010, 46(30): 5488-5490, http://159.226.238.44/handle/321008/141694.
[40] 闫竞宇. 金属氧化物材料的制备及在修饰蛋白质组学中的应用. 2010, [41] Wan, Huihui, Yan, Jingyu, Yu, Long, Zhang, Xiuli, Xue, Xingya, Li, Xiuling, Liang, Xinmiao. Zirconia layer coated mesoporous silica microspheres used for highly specific phosphopeptide enrichment. TALANTA[J]. 2010, 82(5): 1701-1707, http://dx.doi.org/10.1016/j.talanta.2010.07.050.
[42] Yan, Jingyu, Li, Xiuling, Cheng, Sanyan, Ke, Yanxiong, Liang, Xinmiao. Facile synthesis of titania-zirconia monodisperse microspheres and application for phosphopeptides enrichment. CHEMICAL COMMUNICATIONS[J]. 2009, 2929-2931, http://159.226.238.44/handle/321008/141605.