基本信息
叶明亮  男  博导  中国科学院大连化学物理研究所
电子邮件: mingliang@dicp.ac.cn
通信地址: 大连市中山路457号
邮政编码: 116023
部门/实验室:研究生部

导师介绍

叶明亮研究员,博士生导师。2015年国家自然科学基金委杰出青年基金获得者,国家重点研发计划重点专项首席科学家,中科院分离分析化学重点实验室副主任,中国质谱学会常务理事,中国蛋白质组学专业委员会常务委员。2001年在中科院大连化物所获博士学位,2001-2002美国华盛顿大学化学系进行博士后研究,2003-2004 年美国系统生物研究所从事蛋白质组学新方法研究,师从著名蛋白质组学领军人物Ruedi Aebersold教授。2004年回国并入选中科院百人计划并获得择优支持,2016年入选中国科学院特聘研究员、特聘骨干人才和科技部中青年科技创新领军人才。在蛋白质翻译后修饰的高灵敏检测和规模化分析方面发展了多项原创性技术,在Nat. MethodsNat. Chem. Biol., Nat. Protoc.Angew. Chem. Int. Ed等国际高影响期刊发表SCI论文180余篇,引用6000余次。先后主持基金委杰出青年基金、国家重点研发计划重点专项、基金委重点基金、基金委中加健康合作项目、科技部创新方法项目、国家重大科学研究计划课题等项目。曾获中科院院长奖学金特别奖、辽宁省自然科学奖、国家自然科学二等奖等奖励。


招生信息

以色谱分离与质谱分析为立足点,针对蛋白质组等复杂生物样品的高效分离与高灵敏检测问题,开展色谱、质谱分析新技术和新方法的研究,注重解决疾病蛋白质组学研究所面临的技术瓶颈问题,让精准医学更精准。

1)蛋白质质谱分析新技术
发展基于液相色谱与串联质谱联用(LC-MS/MS)的蛋白质分析、鉴定的新方法;发展基于Q-TOF、Orbitrap等高精度质谱确定多肽序列的De Novo技术;发展蛋白质质谱分析的样品预处理技术。

2)蛋白质组修饰谱分析新方法

发展蛋白质磷酸化分析的新方法,包括磷酸化蛋白质和多肽的富集技术、磷酸肽的通量化分离和鉴定技术;发展蛋白质糖基化等其它后修饰的分析新方法。

3)复杂生物样品预处理技术和方法

   基于纳米材料的富集技术和方法、蛋白质组样品预处理的集成化技术、同位素标记技术

4)高效分离柱技术与多维分离分析系统

   高效整体分离柱技术、多维分离分析方法、质谱数据处理方法

5)应用研究

   血液净化材料、转化医学、生物活性肽的筛选与产品创制


070302-分析化学:
(部分方向也欢迎生物、数学专业的同学报考)


出版信息

代表性论文:


1) Jiawei Mao, Xin You, Hongqiang Qin, Chengye Wang, Liming Wang, Mingliang Ye*. A New Searching Strategy for the Identification of O‑Linked Glycopeptides. Analytical Chemistry, 2019, 91, 3852−3859

2) Qi Wang, Zhen Liu, Keyun Wang, Yan Wang, Mingliang Ye*. A new chromatographic approach to analyze methylproteome with enhanced lysine methylation identification performance. Analytica Chimica Acta, 2019, 1068, 111-119

3) Yating Yao, Yan Wang, Shujuan Wang, Xiaoyan Liu, Zhen Liu, Yanan Li, Zheng Fang, Jiawei Mao, Yong Zheng, Mingliang Ye*. One-Step SH2 Superbinder-Based Approach for Sensitive Analysis of Tyrosine Phosphoproteome. Journal of Proteome Research, 2019, 18, 1870−1879

4) Yanan Li, Yan Wang, Jiawei Mao, Yating Yao, Keyun Wang, Qinglong Qiao, Zheng Fang, Mingliang Ye*. Sensitive profiling of cell surface proteome by using an optimized biotinylation method. Journal of Proteomics, 2019, 196, 33-41

5) Xin You, Yating Yao, Jiawei Mao, Hongqiang Qin, Xinmiao Liang, Liming Wang, Mingliang Ye*. Chemoenzymatic Approach for the Proteomics Analysis of Mucin- Type Core‑1 O‑Glycosylation in Human Serum. Analytical Chemistry, 2018, 90, 12714−12722

6) Xin You, Hongqiang Qin, Jiawei Mao, Yu Tian, Mingming Dong, Zhimou Guo, Xinmiao Liang, Liming Wang, Yan Jin, Mingliang Ye*. Highly Efficient Identification of O-GalNAc Glycosylation by an Acid-Assisted Glycoform Simplification Approach. Proteomics, 2018, 18, 1800042

7) Mingming Dong, Yating Yao, Yan Wang, Yan Jin, Mingliang Ye*. The divide and conquer strategies for deep phosphoproteomics analysis. Trends in Analytical Chemistry, 2018, 105, 282-291

8) Jiawen Lyu, Yan Wang, Jiawei Mao, Yating Yao, Shujuan Wang, Yong Zheng, Mingliang Ye*. Pseudotargeted MS Method for the Sensitive Analysis of Protein Phosphorylation in Protein Complexes. Analytical Chemistry, 2018, 90,6214-6221

9) Yating Yao, Yangyang Bian, Mingming Dong, Yan Wang, Jiawen Lv, Lianfang Chen, Hongwei Wang, Jiawei Mao, Jing Dong, Mingliang Ye*. SH2 Superbinder Modified Monolithic Capillary Column for the Sensitive Analysis of Protein Tyrosine Phosphorylation. Journal of Proteome Research, 2018, 17, 243-251

10) Xin You, Hongqiang Qin, Mingliang Ye*. Recent advances in methods for the analysis of protein o-glycosylation at proteome level. Journal of  Separation Sciences, 2018, 41, 248-261

11)  Zhenzhen Deng, Jiawei Mao, Yan Wang, Hanfa Zou, Mingliang Ye*. Enzyme kinetics for complex system enables accurate determination of specificity constants of numerous substrates in a mixture by proteomics platform. Mol. Cell. Proteomics, 2017, 16(1), 135-145

12)  Yanan Li, Yan Wang, Mingming Dong, Hanfa Zou, Mingliang Ye*. Sensitive Approaches for the Assay of the Global Protein Tyrosine Phosphorylation in Complex Samples Using a Mutated SH2 Domain. Anal. Chem., 2017, 89 (4), 2304–2311

13) Zhenzhen Deng, Mingming Dong, Yan Wang, Jing Dong, Shawn S-C Li, Hanfa Zou, Mingliang Ye*. Biphasic Affinity Chromatographic Approach for Deep Tyrosine Phosphoproteome Analysis. Anal. Chem. 2017, 89(4), 2405−2410

14)  Xuefang Dong, Hongqiang Qin, Jiawei Mao, Dongping Yu, Xiuling Li, Aijin Shen, Jingyu Yan, Long Yu, Zhimou Guo, Mingliang Ye*, Hanfa Zou, Xinmiao Liang*. In-depth Analysis of Glycoprotein Sialylation in Serum Using a Dual-Functional Material with Superior Hydrophilicity and Switchable Surface Charge.  Anal. Chem., 2017(7), 89, 3966–3972

15)  Haiyang Zhang, Junjie Ou*, Yating Yao, Hongwei Wang, Zhongshan Liu, Yinmao Wei*, and Mingliang Ye*. Facile Preparation of Titanium(IV)-Immobilized Hierarchically Porous Hybrid Monoliths. Anal. Chem., 2017, 89(8), 4655–4662

16) Yuting Cong, Lianghai Hu*, Zhang Zhang, Yin Gao, Mingming Dong, Hongqiang Qin, Mingliang Ye*, Jingkai Gu, Hanfa Zou. Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study. Talanta, 2017, 165, 664–670

17)  Hongqiang Qin, Kai Cheng, Jun Zhu, Jiawei Mao, Fangjun Wang, Mingming Dong, Rui Chen, Zhimou Guo, Xinmiao Liang, Mingliang Ye*, Hanfa Zou. Proteomics Analysis of O-GalNAc Glycosylation in Human Serum by an Integrated Strategy, Anal. Chem., 2017, 89(3):1469-1476

18) Yangyang Bian, Lei Li, Mingming Dong, Xuguang Liu, Tomonori Kaneko, Kai Cheng, Huadong Liu, Courtney Voss, Xuan Cao, Yan Wang, David Litchfield, Mingliang Ye*, Shawn SC Li*, Hanfa Zou*. Ultra-deep tyrosine phosphoproteomics enabled by a phosphotyrosine superbinder. Nat. Chem. Biol., 2016, 12, 959-968

19) Keyun Wang, Mingming Dong, Jiawei Mao, Yan Wang, Yan Jin, Mingliang Ye*, Hanfa Zou. Antibody-Free Approach for the Global Analysis of Protein Methylation.Anal. Chem., 2016, 2016, 88(23), 11319-11327

20) Ye, Mingliang*; Pan, Yanbo; Cheng, Kai; Zou, Hanfa*. Protein Digestion Priority is Independent of Protein Abundances. Nat. Methods, 2014, 11: 220-222.

21) Pan, Yanbo; Ye, Mingliang*; Zhao, Liang; Cheng, Kai; Dong, Mingming; Song, Chunxia; Qin,Hongqiang; Wang, Fangjun; Zou, Hanfa*. N-Terminal Labeling of Peptides by Trypsin-Catalyzed Ligation for Quantitative Proteomics. Angew. Chem. Int. Ed., 2013, 52(35): 9205-9209.




 

 



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