Jian Hao do researches about bio-base chemicals, especially focus on  using Klebsiella pneumoniae as the  cell factory. He have done some researches about the mechanism of 1,3-propaediol  and 2,3-butanediol synthesis by K.  pneumoniae. Besides, He have developed 2-ketogluconic acid, gluconic acid, xylonic  acid, acetoin, isobutanol and 2-ketoisovalerate production by this bacterium.

PhD, Chemical Engineering, Tsinghua University, China 2003-2007.

M.S, Food Engineering, Tianjin Institute of Light Industry, China, 1997-2000.

B.S, Fermentation, Tianjin Institute of Light Industry, China, 1993-1997

2010 to date: Associate Professor, Full Professor, Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, China.

2008-2010: Associate Professor, Department of Environmental and Chemical Engineering Yanshan University, Qinhuangdao China.

2007-2008: Postdoctoral Researcher, Department of Biochemical Engineering, University College London, UK.

2000-2003: Lecture, Department of Environmental and Chemical Engineering Yanshan University, Qinhuangdao China.

   

[1] Shu Lin, Gu Jinjie, Wang Qinghui, Sun Shaoqi, Cui Youtian, Fell Jason, Mak Wai Shun, Siegel Justin B. Shi Jiping, Lye Gary J, Baganz Frank*, Hao Jian*. The pyruvate decarboxylase activity of IpdC is a limitation for isobutanol production by Klebsiella pneumoniae. Biotechnology for Biofuels and Bioproducts 2022. 15:41 (Q1 6.04)

[2] Wang Qinghui, Gu Jinjie, Shu Lin, Jiang Weiyan, Mojovic Ljiljana, Knezevic-Jugovic Zorica, Shi Jiping, Baganz Frank, Lye Gary J, Xiang Wensheng*, Hao Jian *. Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in L-valine production. World Journal of Microbiology and Biotechnology 2022. 38:81 (Q2 3.312)

[3] Shu Lin, Wang Qinghui, Jiang Weiyan, Marina Tišma, Oh Beakrock, Shi Jiping, Lye Gary, Baganz Frank, Wei Dong*, Hao Jian*. The roles of diol dehydratase from pdu operon on glycerol catabolism in Klebsiella pneumoniae. Enzyme and Microbial Technology. 2022. 157: 110021 (Q2 3.493)

[4] Sun Shaoqi, Shu Lin, Lu Xiyang, Wang Qinghui, Marina Tišma, Zhu Chenguang, Shi Jiping, Lye Gary, Baganz Frank*, Hao Jian*. 1,2‑Propanediol production from glycerol via an endogenous pathway of Klebsiella pneumoniae. Applied Microbiology and Biotechnology. 2021. 105: 9003-9016 (Q1 4.813)

[5] Sun Shaoqi, Wang Yike , Shu Lin, Lu Xiyang, Wang Qinghui, Zhu Chenguang, Shi Jiping, Lye Gary, Baganz Frank*, Hao Jian*. Redirection of the central metabolism of Klebsiella pneumoniae towards dihydroxyacetone production. Microbial Cell Factories. 2021. 20: 123. (Q1 5.328)

[6] Yang Yang#, Zhang Zhongxi#, Lu Xiyang, Gu Jinjie, Wang Yike, Yao Yao, Liao Xianyan, Shi Jiping, Lye Gary, Baganz Frank*, Hao Jian*. Production of 2,3-dihydroxyisovalerate by Enterobacter cloacae. Enzyme and Microbial Technology. 2020 (140): 109650. (Q2 3.493)

[7] Lu Xiyang, Yao Yao, Yang Yang, Zhang Zhongxi, Gu Jinjie, Ljiljana Mojovic, Zorica Knezevic-Jugovic, Baganz Frank, Lye Gary, Shi Jiping*, Hao Jian*. Ethylene glycol and glycolic acid production by wild-type Escherichia coli. Biotechnology and Applied Biochemistry. 2021. 68(4): 744-755.  (Q3 2.431)

[8] Wang Yike#, Gu Jinjie#, Lu Xiyang, Zhang Zhongxi, Yang Yang, Sun Shaoqi, Emily T. Kostas, Shi Jiping, Gao Mintian, Baganz Frank*, Lye Gary*, Hao Jian*. 2,3-Dihydroxyisovalerate production by Klebsiella pneumoniae. Applied Microbiology and Biotechnology 2020. 104(15): 6601–6613.  (Q1 4.813)

[9] Zhang Zhongxi#, Yang Yang#, Wang Yike, Gu Jinjie, Lu Xiyang, Liao Xianyan, Shi Jiping, Kim Chulho, Lye Gary, Baganz Frank*, Hao Jian*. Ethylene glycol and glycolic acid production from xylonic acid by Enterobacter cloacae. Microbial Cell Factories. 2020. 19:89. (cited 7) (Q1 5.328)

[10] Dong Wei, Yuki Yuminaga, Jiping Shi, Hao Jian*. Non-capsulated mutants of a chemical-producing Klebsiella pneumoniae strain. Biotechnology Letter 2018. 40 (4): 1-9.  (Q3 2.461)

[11] Gu Jinjie, Zhou Jidong, Zhang Zhongxi, Kim Chul Ho, Jiang Biao, Shi Jiping*, Hao Jian*. Isobutanol and 2-ketoisovalerate production by Klebsiella pneumoniae via a native pathway. Metabolic Engineering 2017. 43:71-84.  (Q1 9.783)

[12] Wei Dong, Gu Jinjie, Zhang Zhongxi, Wang Chenhong, Wang Dexin, Kim Chul Ho, Jiang Biao, Shi Jiping*, Hao Jian*. Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock. J. Vis. Exp. 2017. (124), e55828. (Q3 1.355)

[13] Zhou Jidong, Wang Dexin, Wang Chenhong, Gu Jinjie, Kim Chul Ho, Shi Jiping, Jiang Biao, Wang Min*, Hao Jian*. The role of the pyruvate acetyl-CoA switch in the production of 1,3-propanediol by Klebsiella pneumoniae. Applied Biochemistry and Biotechnology, 2017. 181:1199-1210.  (Q3 2.926)

[14] Wang Chenhong, Wei Dong, Zhang Zhongxi, Wang Dexin, Shi Jiping, Kim Chul Ho, Jiang Biao, Han Zengsheng*, Hao Jian*. Production of xylonic acid by Klebsiella pneumoniae. Applied Microbiology and Biotechnology, 2016. 100, 23, 10055-10063. (Q1 4.813)

[15] Wang Dexin, Wang Chenhong, Wei Dong, Shi Jiping, Kim Chul Ho, Jiang Biao, Han Zengsheng* Hao Jian*.Gluconic acid production by gad mutant of Klebsiella pneumoniae World J Microbiol Biotechnol, 2016, 32:132.  (Q2 3.312)

[16] Chen Chuan, Wei Dong, Liu Pengfu, Wang Min, Shi Jiping*, Jiang Biao, Hao Jian*. Inhibition of RecBCD in Klebsiella pneumoniae by Gam and its effect on the efficiency of gene replacement. Journal of Basic Microbiology, 2016. 56: 120-126. (Q4 2.281)

[17] Wang Dexin, Zhou Jidong, Chen Chuan, Wei Dong, Shi Jiping, Jiang Biao, Liu Pengfu, Hao Jian*. R-acetoin accumulation and dissimilation in Klebsiella pneumoniae. Journal of Industrial Microbiology & Biotechnology, 2015. 42(8):1105-1115. (Q2 3.346)

[18] Sun Yuehong, Wei Dong, Shi Jiping, Mojović Ljiljana, Han Zengsheng*, Hao Jian*. Two-stage fermentation for 2-ketogluconic acid production by Klebsiella pneumoniae. Journal of Microbiology and Biotechnology, 2014. 24(6):781–787.  (Q3 2.351)

[19] Wei Dong, Wang Min, Jiang Biao, Shi Jiping*, Hao Jian*. Role of dihydroxyacetone kinases I and II in the dharegulon of Klebsiella pneumoniae. Journal of Biotechnology, 2014. 177:13–19. (Q2 3.307)

[20] Chen Chuan, Wei Dong, Shi Jiping, Wang Min*, Hao Jian*. Mechanism of 2,3-butanediol stereoisomer formation in Klebsiella pneumoniae. Applied Microbiology and Biotechnology, 2014. 98 (10):4603–4613. (Q1 4.813)

[21] Wei Dong, Xu Jiqing, Sun Junsong, Shi Jiping, Hao Jian*. 2-Ketogluconic acid production by Klebsiella pneumoniae CGMCC 1.6366. Journal of Industrial Microbiology & Biotechnology. 2013. 40(6):561-570. (Q2 3.346)

[22] Wei Dong, Sun Junsong, Shi Jiping, Liu Pengfu* Hao Jian*. New strategy to improve efficiency for gene replacement in Klebsiella pneumoniae．Journal of Industrial Microbiology & Biotechnology. 2013. 40(5):523-527.  (Q2 3.346)

[23] Wei Dong, Wang Min, Shi Jiping, Hao Jian*. Red recombinase assisted gene replacement in Klebsiella pneumoniae．Journal of Industrial Microbiology & Biotechnology. 2012 39(8):1219–1226.  (Q2 3.346)

[24] Hao Jian, Lin Rihui, Zheng Zongming, Sun Yan, Liu Dehua. 3-Hydroxypropionaldehyde guided glycerol feeding strategy in aerobic 1,3-propanediol production by Klebsiella pneumoniae. Journal of Industrial Microbiology & Biotechnology. 2008, 35:1615–1624. (Q2 3.346)

[25] Hao Jian, Wang Wei, Tian Jiesheng, Li Jilun, Liu Dehua. Decrease of 3-hydroxypropionaldehyde accumulation in 1,3-propanediol production by over-expressing dhaT gene in Klebsiella pneumoniae TUAC01. Journal of Industrial Microbiology & Biotechnology. 2008, 35:735-741. (Q2 3.346)

[26] Hao Jian, Lin Rihui, Zheng Zongming, Liu Hongjuan, Liu Dehua. Isolation and characterization of microorganisms able to produce 1,3-propanediol under aerobic conditions. World Journal of Microbiology Biotechnology 2008, 24:1731-1740. (Q2 3.312)

[27] Hao Jian, Xu Feng, Liu Hongjuan, Liu Dehua. Downstream processing of 1,3-propanediol fermentation broth. Journal of Chemical Technology and Biotechnology. 2006, 81: 102-108 (Q3 3.174)

[28] Hao Jian, Liu Hongjuan, Liu Dehua. Novel route of reactive extraction to recover 1,3-propanediol from a dilute aqueous systems. Industrial and Engineering Chemistry Research. 2005, 44: 4380-4385 (Q2 3.764)

[29] 姚尧，卢希阳，舒 琳，王卿惠，孙少琦，郝健*，以葡萄糖为原料结合生物发酵与化学合成制备酮泛解酸，过程工程学报，2022, 22 (1): 97-107

[30] 孙少琦，王轶可，杨 洋，朱晨光*，史吉平，郝健*，Rcs信号系统对克雷伯氏肺炎杆菌荚膜合成的影响，过程工程学报，2021, 21 (2): 230-239

[31] 白旭皓，杨洋，董永华，廖鲜艳，郝健*，糖酸类化合物的解磷机理研究，江苏农业科学，2020，48(09)：269-275

[32]张忠喜，顾金杰，林汉标，廖鲜艳，史吉平，郝健*，基于Red 重组系统的阴沟肠杆菌基因重组技术，食品工业科技，2018, 39 (21):134-140,151。

[33] 周吉东，郝晓蔚，王敏，史吉平，郝健*，克雷伯肺炎杆菌2,3-丁二醇合成途径功能基因研究，山东农业大学学报(自然科学版)，2018,49(1):1-8。

[34] 柳鹏福，王晨红，储消和，史吉平，郝健*，克雷伯氏肺炎杆菌群体感应系统缺失对合成2,3-丁二醇的影响，华中农业大学学报，2017，1：61-67

[35] 魏东 王敏 庄文超 史吉平，郝健*，克雷伯氏肺炎杆菌电击转化条件的优化，中国酿造，2012，04：18-21。

[36] 刘海平，郝健*，大肠杆菌中1,3—丙二醇氧化还原酶的表达与纯化，生物技术，2011,21（1）：19-23。

[37] 郝健，刘德华，1,3-丙二醇发酵液电渗析法脱盐，过程工程学报，2005，5：36-39

已指导学生

颜薇芝  硕士研究生  085238-生物工程  

魏东  博士研究生  081703-生物化工  

姚尧  硕士研究生  085238-生物工程  

舒琳  硕士研究生  085238-生物工程  

现指导学生

降伟彦  硕士研究生  081703-生物化工  

蔡瑶雨  硕士研究生  086000-生物与医药  

孙少琦  博士研究生  081703-生物化工  

王文琪  硕士研究生  081703-生物化工