基本信息
冯银刚  男  博导  中国科学院青岛生物能源与过程研究所
电子邮件: fengyg@qibebt.ac.cn
通信地址: 青岛市崂山区松岭路189号
邮政编码: 266101

研究领域

重要工业微生物蛋白质的质结构、功能和蛋白质工程; 
工业微生物的代谢工程;
生物大分子核磁共振。

招生信息

   
招生专业
071010-生物化学与分子生物学
招生方向
结构生物学与蛋白质工程

教育背景

1999-09--2004-06   中国科学院生物物理研究所   博士学位
1995-09--1999-06   南京大学物理学系   学士学位
学历
-- 研究生
学位
-- 博士

工作经历

   
工作简历
2011-06~现在, 中国科学院青岛生物能源与过程研究所, 研究员
2006-07~2011-05,中国科学院生物物理研究所, 副研究员
2004-07~2006-06,北京大学化学与分子工程学院, 博士后
1999-09~2004-06,中国科学院生物物理研究所, 博士学位
1995-09~1999-06,南京大学物理学系, 学士学位
社会兼职
2014-04-20-今,中国生物物理学会-生物磁共振专业委员会委员,

出版信息

   
发表论文
(1) Integration of bacterial expansin-like proteins into cellulosome promotes the cellulose degradation., Appl. Microbiol. Biotechnol., 2016, 通讯作者
(2) NMR structure note: the C-terminal region of human eukaryotic elongation factor 1Bδ., J. Biomol. NMR, 2016, 通讯作者
(3) Low stability of the reduced state of Mycobacterium tuberculosis NrdH redoxin., FEBS Lett., 2016, 通讯作者
(4) Bcl-2/MDM2 dual inhibitors based on universal pyramid-like α-helical mimetics., J. Med. Chem., 2016, 第 3 作者
(5) 利用伯克霍尔德氏菌脂肪酶富集裂壶藻油脂中的DHA, 食品科学, 2015, 通讯作者
(6) Evolutionarily conserved binding of translationally-controlled tumor protein to eukaryotic elongation factor 1B. , J. Biol. Chem., 2015, 通讯作者
(7) Simultaneous accumulation of neutral lipids and biomass in Nannochloropsis oceanica IMET1 under high light intensity and nitrogen replete conditions. , Algal Res., 2015, 通讯作者
(8) Resonance assignments of the periplasmic domain of a cellulose-sensing trans-membrane anti-sigma factor from Clostridium thermocellum, Biomol. NMR Assign., 2015, 通讯作者
(9) 1H, 15N and 13C resonance assignments of translationally-controlled tumor protein from photosynthetic microalga Nannochloropsis oceanica., Biomol. NMR Assign., 2015, 通讯作者
(10) High production of squalene using a newly isolated yeast-like strain Pseudozyma sp. SD301., J. Agric. Food Chem., 2015, 第 4 作者
(11) Transcriptome and gene expression analysis of DHA producer Aurantiochytrium under low temperature conditions., Sci. Rep., 2015, 第 4 作者
(12) Revisiting the NMR solution structure of the Cel48S type-I dockerin module from Clostridium thermocellum reveals a cohesin-primed conformation., J. Struct. Biol., 2014, 通讯作者
(13) Structural basis of X chromosome DNA recognition by the MSL2 CXC domain during Drosophila dosage compensation, Genes Dev., 2014, 第 4 作者
(14) 热纤梭菌中心代谢产物的归属及初步代谢组分析, 波谱学杂志, 2014, 第 3 作者
(15) 裂殖壶菌SD116油脂的胞内核磁共振研究, 生物物理学报, 2014, 通讯作者
(16) Improvement of ClosTron for successive gene disruption in Clostridium cellulolyticum using a pyrF-based screening system., App. Microbiol. Biotechnol. , 2014, 通讯作者
(17) Retinoblastoma-binding protein 1 has an interdigitated double Tudor domain with DNA-binding activity., J. Biol. Chem., 2014, 通讯作者
(18) Structural and biochemical characterization reveals LysGH15 as an unprecedented EF-hand-like calcium-binding phage lysin, PLoS Pathog. , 2014, 第 2 作者
(19) Mechanism of the Rpn13-induced activation of Uch37, Protein Cell , 2014, 通讯作者
(20) Type AII lantibiotic bovicin HJ50 with a rare disulfide bond: structure, structure-activity relationships and mode of action, Biochem. J. , 2014, 第 1 作者
(21) Two-face, two-turn α-helix mimetics based on a cross-acridine scaffold: analogues of the Bim BH3 domain.,  ChemBioChem , 2014, 第 3 作者
(22) The contribution of cellulosomal scaffoldins to cellulose hydrolysis by Clostridium thermocellum analyzed by using thermotargetrons. , Biotechnol. Biofuels , 2014, 第 3 作者
(23) Structure determination of archaea-specific ribosomal protein L46a reveals a novel protein fold., Biochem. Biophys. Res. Commun., 2014, 通讯作者
(24) Metabolic adaption of ethanol-tolerant Clostridium thermocellum., PLoS One, 2013, 第 3 作者
(25) Anti-apoptosis proteins Mcl-1 and Bcl-xL have different p53-binding profiles., Biochemistry, 2013, 通讯作者
(26) An RNA-binding complex involved in ribosome biogenesis contains a protein with homology to tRNA CCA-adding enzyme., PLoS Biol., 2013, 第 3 作者
(27) Separation and quantification of water-soluble cellular metabolites in Clostridium thermocellum using liquid chromatography-isotope dilution tandem mass spectrometry., Anal. Lett., 2013, 通讯作者
(28) NMR structure note: a PilT N-terminus domain protein SSO1118 from hyperthemophilic archaeon Sulfolobus solfataricus P2. , J. Biomol. NMR, 2013, 通讯作者
(29) Resonance assignments of cohesin and dockerin domains from Clostridium acetobutylicum ATCC824, Biomol. NMR Assign., 2013, 第 4 作者
(30) Metabolic profiles of Nannochloropsis oceanica IMET1 under nitrogen-deficiency stress, Bioresour. Technol. , 2013, 第 4 作者
(31) Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp. SD116. , J. Oleo Sci., 2013, 第 3 作者
(32) Modeling of the [E43S]SNase-ssDNA-Cd2+ complex: structural insight into the action of nuclease on ssDNA, Arch. Biochem. Biophys. , 2013, 第 2 作者
(33) Structural insights into the N-terminal GIY-YIG endonuclease activity of Arabidopsis glutaredoxin AtGRXS16 in chloroplasts., Proc. Natl. Acad. Sci. USA , 2013, 通讯作者
(34) Novel soluble myeloid cell leukemia sequence 1 (Mcl-1) inhibitor (E,E)-2-(benzylaminocarbonyl)-3-styrylacrylonitrile (4g) developed using a fragment-based approach. , Eur. J. Med. Chem., 2013, 通讯作者
(35) The archaeal Sac10b protein family: conserved proteins with divergent functions, Curr. Protein Pept. Sci., 2012, 通讯作者
(36) 古菌蛋白质SSO6904的双体形式的NMR鉴定, Dimer form of archaeal protein SSO6904 identified by NMR, 波谱学杂志, 2012, 通讯作者
(37) Structural insight into recognition of methylated histone tails by retinoblastoma-binding protein 1, J. Biol. Chem., 2012, 通讯作者
(38) NMR studies of the interaction between human programmed cell death 5 and human p53, Biochemistry, 2012, 第 2 作者
(39) Glutathione regulates the transfer of iron-sulfur cluster from monothiol and dithiol glutaredoxins to apo ferredoxin, Protein Cell , 2012, 第 4 作者
(40)  Targeted gene engineering in Clostridium cellulolyticum H10 without methylation., J. Microbiol. Methods, 2012, 通讯作者
(41) NMR structure note: human esophageal cancer-related gene 2, J. Biomol. NMR, 2012, 第 1 作者
(42) Solution structure of MSL2 CXC domain reveals an unusual Zn3Cys9 cluster and similarity to pre-SET domains of histone lysine methyltransferases, PLoS One, 2012, 通讯作者
(43) Efficiency and stability enhancement of cis-epoxysuccinic acid hydrolase by fusion with a carbohydrate binding module and immobilization onto cellulose, Appl. Biochem. Biotechnol., 2012, 通讯作者
(44) High yield recombinant expression, characterization and homology modeling of two types of cis-epoxysuccinic acid hydrolases., Protein J. , 2012, 通讯作者
(45) An anthraquinone scaffold for putative, two-face Bim BH3 α-helix mimicries, J. Med. Chem., 2012, 通讯作者
(46) Molecular basis of Wnt activation via the DIX-domain protein Ccd1, J. Biol. Chem., 2011, 第 4 作者
(47) Resonance assignments of a putative PilT N-terminus domain protein SSO1118 from hyperthermophilic archaeon Sulfolobus solfataricus P2., Biomol. NMR Assign. , 2011, 通讯作者
(48) Protein-protein complexation in bioluminescence, Protein Cell, 2011, 第 2 作者
(49) NMR derived topology of a GFP-photoprotein energy transfer complex, J. Biol. Chem., 2010, 第 1 作者
(50) Binding of NADPH destabilizes the iron-sulfur clusters of human mitoNEET. , Biochemistry, 2010, 第 3 作者
(51) Crystal Structure of the crenarchaeal conserved chromatin protein Cren7 and double-stranded DNA complex., Protein Sci., 2010, 第 1 作者
(52) Importance of the C-terminal loop L137-S141 for the folding and folding stability of staphylococcal nuclease, Biochemistry, 2010, 第 2 作者
(53) Solution structure and calcium binding of protein SSO6904 from the hyperthermophilic archaeon Sulfolobus solfataricus, Proteins, 2010, 通讯作者
(54) The native-like interactions between SNase121 and SNase(111-143) fragments induce the recovery of their native-like structures and the ability to degrade DNA, Biochemistry, 2009, 第 1 作者
(55) Structure-function correlation of human programmed cell death 5 protein, Arch. Biochem. Biophys. , 2009, 第 3 作者
(56)  Cloning, expression and purification of DNA binding protein Mvo10b from Methanococcus voltae. , Protein Expr. Purif. , 2009, 第 3 作者
(57) Solution structure of synbindin atypical PDZ domain and interaction with syndecan-2. , Protein Peptide Lett., 2009, 第 1 作者
(58) Favorable contribution of the C-terminal residue K97 to the stability of a hyperthermophilic archaeal [P62A]Ssh10b. , Arch. Biochem. Biophys. , 2009, 第 2 作者
(59) A stabilizing alpha/beta-hydrophobic core greatly contributes to hyperthermostability of archaeal [P62A]Ssh10b. , Biochemistry, 2008, 第 4 作者
(60) Mapping the putative binding site for uPA protein in Esopageal Cancer Related Gene 2 by heteronuclear NMR method. , Arch. Biochem. Biophys. , 2008, 第 2 作者
(61)  Biochemical and structural characterization of Cren7, a novel chromatin protein conserved among Crenarchaea. , Nucleic Acid Res. , 2008, 第 1 作者
(62) Solution structure and mapping of a very weak calcium-binding site of human translationally controlled tumor protein by NMR. , Arch. Biochem. Biophys., 2007, 第 1 作者
(63) Folding of the C-terminal fragment V111-D143 of staphylococcal nuclease in aqueous solution., Protein Peptide Lett., 2007, 第 4 作者
(64) Folding stability and cooperativity of the three forms of 1-110 residues fragment of staphylococcal nuclease. , Biophys. J., 2007, 第 3 作者
(65) 谷氧还蛋白系统及其对细胞氧化还原态势的调控, 生物物理学报, 2007, 第 2 作者
(66) Structural insight into poplar glutaredoxin C1 with a bridging iron-sulfur cluster at the active site, Biochemistry, 2006, 第 1 作者
(67) The N-terminal 26-residue fragment of human programmed cell death 5 protein can form a stable alpha-helix having unique electrostatic potential character. , Biochem. J., 2005, 第 4 作者
(68) Letter to the Editor: 1H, 15N, and 13C Resonance assignments of reduced glutaredoxin C1 from Populus tremula x tremuloides., J. Biomol. NMR, 2005, 第 1 作者
(69) Searching for folding initiation sites of staphylococcal nuclease: a study of N-terminal short fragments. , Biopolymers, 2004, 第 3 作者
(70) Human programmed cell death 5 protein has a helical-core and two dissociated structural regions., Biochem. Biophys. Res. Commun. , 2004, 第 2 作者
(71) Two conformations of archaeal Ssh10b: the origin of its temperature-dependent interaction with DNA., J. Biol. Chem., 2003, 通讯作者
(72) Native-like partially folded conformations and folding process revealed in the N-terminal large fragments of staphylococcal nuclease: a study by NMR spectroscopy., J. Mol. Biol., 2003, 第 1 作者
(73) Letters to the Editor: 1H, 15N, and 13C resonance assignments and secondary structure of the Ssh10b from hyperthermophilic archaeon Sulfolobus shibatae. , J. Biomol. NMR, 2002, 第 3 作者

科研活动

   
科研项目
( 1 ) 梭菌的纤维小体组装模块的结构、相互作用与理性设计的核磁共振研究, 主持, 国家级, 2013-01--2016-12
参与会议
(1)Structure and Function of Transmembrane Extracytoplasmic Function (ECF) Anti-sigma Factors for Cellulosome Regulation in Clostridium thermocellum   第十四届中国暨国际生物物理大会   2015-11-19
(2)NMR Structures of Cellulosomal Cohesin and Dockerin from Clostridium acetobutylicum    第十九届国际磁共振学会会议   2015-08-17
(3)热纤梭菌中anti-sigma因子的核磁共振研究   第十八届全国波谱学学术会议   2014-10-10
(4)癌症抑制相关蛋白ARID4A的结构与相互作用研究   中国生物物理学会生物磁共振专业委员会成立大会暨第一届全国生物磁共振研讨会   2014-04-18