张长生 男 博导 南海海洋研究所
电子邮件:czhang@scsio.ac.cn;czhang2006@gmail.com
通信地址:广州市新港西路164号
邮政编码:510301

ORCID: https://orcid.org/0000-0003-2349-3138

研究领域和科研项目

研究领域

海洋微生物的分离鉴定、活性次生代谢产物的天然发现、生物合成和结构改造等方面的研究工作,探索结构新颖、活性独特的微生物次生代谢产物的生物合成途径和所蕴藏的新颖生物合成酶反应机制及其在天然药物结构多样化方面的应用。

1. 微生物天然活性物质的分离鉴定。采用微生物学和化学手段,从不同环境(主要是特殊环境的海洋生态系统,包括海底沉积物、珊瑚礁和海绵)中筛选分离特殊的微生物(主要包括放线菌和真菌),并通过发酵和活性筛选从微生物中分离和鉴定具有生物活性的小分子化合物或者大分子复合物。

2. 复杂微生物次生代谢产物的生物合成研究。用分子生物学手段克隆完整的生物合成基因簇,研究生物合成途径,进行基因遗传操作(包括基因阻断、置换和重组等),用组合生物合成的手段创造“非天然”的天然化合物,筛选新的生物活性。课题组已展开了针对新型抗菌素和抗肿瘤药物如友菌素(amicetin)、台勾霉素(tiacumicin)、浅蓝霉素(caerulomicin)、Elaiophylin、lobophorins、DNQ和斑鸠霉素(ikarugamycin)等多种抗生素的生物合成工作。

3.微生物次生代谢产物新功能酶的作用机制及其应用。用生物化学手段来进行体外研究并揭示复杂天然产物生物合成中所包含的独特酶学机制,开发这些酶(如糖基转移酶,甲基化酶,氧化还原酶等)在天然产物结构多样化方面的应用潜力。


科研项目

国家自然科学基金委

              杰出青年基金 2012-2015

              重点项目 2017-2021

              重点国际(地区)合作研究项目 2019-2023

              面上项目(3)

国家重点基础研究发展计划(973计划)课题  2010-2014

中科院前沿领域重点项目 2016-2021

重要方向性项目 2009-2011

中科院先导专项重点任务 2013-2017

广东省海洋经济创新发展区域示范项目,课题 2013-2017

广东省自然基金重大培育项目 2015-2018

广东省特支计划人才项目(2) 2015-2019

广东省渔港建设和渔业产业发展专项 2016-2018

招生信息

   
招生专业
070703-海洋生物学
070321-化学生物学
071005-微生物学
招生方向
海洋生物学
海洋天然产物化学
海洋微生物学

教育背景

1998-09--今 德国乌帕塔尔大学 博士
1994-09--今 华东理工大学 硕士
1990-08--今 上海交通大学 学士

工作经历

2020/08  --  今                 中国科学院南海海洋研究所,副所长

2015/04  --  2020/07       中国科学院南海海洋研究所,所长助理

2012/08  --  2020/04       中国科学院南海海洋研究所,科研与规划处处长

2008/03  --  今                 中国科学院南海海洋研究所, 研究员

2003/01  --  2008/03       美国威斯康星大学药学院博士后,assistant Scientist

1997/07  --  1998/08      上海太平洋生物高科技有限公司,助理研究员       

教授课程

海洋生物资源可持续利用讲座

获得荣誉

2020年:  2020年度中国科学院南海海洋研究所优秀研究生指导教师荣誉称号

2019年:  Editorial board member, Natural Product Reports

2018年:  2018年度"中国科学院优秀导师奖"

2018年:  2018年度中国科学院广州教育基地"优秀研究生导师"

2017年:  享受“国务院政府特殊津贴”

2016年:  国家科技创新领军人才

2016年:   第四届曾呈奎海洋科技奖--青年科技奖,中国海洋湖沼学会

2015年:  广东特支计划“杰出人才(南粤百杰)”

2014年:  有突出贡献中青年专家

2014年:  中青年科技创新领军人才

2014年:  广东特支计划”领军人才”

2013年:  择优终期评估”优秀”,中国科学院

2012年:   全国优秀科技工作者,中国科协

2011年:   国家杰出青年基金获得者(终期评估优秀),国家自然科学基金委员会

2009年:   择优支持入选者,中国科学院 

2005年: “First Midwest Carbohydrate Symposium”, Anatrace Best Poster Award  

出版信息

# (co-first author), * (corresponding author)

2020

Zhuangjie Fang,# Xiaodong Jiang,# Qingbo Zhang,* Liping Zhang, Wenjun Zhang, Chunfang Yang, Haibo Zhang, Yiguang Zhu,Changsheng Zhang.* S-Bridged Thioether and Structure-diversified Angucycline Derivatives from the South China Sea-Derived Micromonospora echinospora SCSIO 04089. Journal of Natural Products, 2020, accepted (DOI: 10.1021/acs.jnatprod.0c00719).


Jinxin Xu,#,* Xiaowen Tang,# Yiguang Zhu, Zhijun Yu, Kai Su, Yulong Zhang, Yan Dong, Weiming Zhu, Changsheng Zhang, Ruibo Wu, Jinsong Liu.* Structural studies reveal flexible roof of active site responsible for ω-transaminase CrmG overcoming by-product inhibition. Communications Biology, 2020, DOI: 10.1038/s42003-020-01184-w.


Liang Ma, Wenjun Zhang, Zhiwen Liu, Yanbing Huang, Qingbo Zhang, Xinpeng Tian, Changsheng Zhang, Yiguang Zhu.* Complete genome sequence of Streptomyces sp. SCSIO 03032 isolated from Indian Ocean sediment,  producing diverse bioactive natural products. Marine Genomics, 2020, DOI: 10.1016/j.margen.2020.100803.


Chunyan Fang, Yiguang Zhu, Changsheng Zhang, and Qingbo Zhang*. The study of secondary metabolites from the PPtase overexpressed marine-derived actinomycete Streptomyces sp. SCSIO 40060 [in Chinese]. Chinese Journal of Marine Drugs, 2020, accepted.


Imran Khan, Haibo Zhang,* Wei Liu,  Liping Zhang, Fang Peng, Yuchan Chen, Qingbo Zhang, Guangtao Zhang, Weimin Zhang, and Changsheng Zhang.* Identification and bioactivity evaluation of secondary metabolites from Antarctic-derived Penicillium chrysogenum CCTCC M 2020019.  RSC Advances, 2020, 10 (35): 20738-20744. DOI:  10.1039/D0RA03529G.

Ling Liu, Sainan Li, Runze Sun, Xiangjing Qin, Jianhua Ju, Changsheng Zhang, Yanwen Duan,* and Yong Huang.* Activation and characterization of bohemamine biosynthetic gene cluster from Streptomyces sp. CB02009. Organic Letters, 2020, 22 (12): 4614–4619. DOI: 10.1021/acs.orglett.0c01224.

Yuanqi Wang, Changsheng Zhang, Yilei Zhao*, Rosalinda Zhao, Kendall Houk.  Understand the specific regio- and enantioselectivity of fluostatin  conjugation in the post-biosynthesis. Biomolecules, 2020, 10 (6): 815. DOI: 10.3390/biom10060815.


Yiguang Zhu,# Qingbo Zhang,# Chunyan Fang, Yingli Zhang, Liang Ma, Zhiwen Liu, Shilan Zhai, Jing Peng, Liping Zhang, Weiming Zhu,* and Changsheng Zhang.* Refactoring the concise biosynthetic pathway of cyanogramide unveils spirooxindole formation catalyzed by a P450 enzyme. Angewandte Chemie International Edition, 2020,  DOI: 10.1002/anie.202004978 and 10.1002/ange.202004978.


Wei Liu,‡ Vinay Gopal Jannu,‡ Zhiwen Liu, Qingbo Zhang, Xiaodong Jiang, Liang Ma, Wenjun Zhang, Changsheng Zhang* and Yiguang Zhu*. Heterologous expression of the trichostatin gene cluster and functional characterization of the N-methyltransferase TsnB8. Organic & Biomolecular Chemistry, 2020, 18 (19): 3649-3653. DOI: 10.1039/d0ob00617c.


Sisi Kang, Mei Yang, Zhongsi Hong, Liping Zhang, Zhaoxia Huang, Xiaoxue Chen, Suhua He, Ziliang Zhou, Zhechong Zhou, Qiuyue Chen, Yan Yan, Changsheng Zhang, Hong Shan,* and Shoudeng Chen.* Crystal structure of SARS-CoV-2 nucleocapsid protein RNA binding domain reveals potential unique drug targeting sites. Acta Pharmaceutica Sinica B (APSB), 2020, DOI: 10.1016/j.apsb.2020.04.009.


Chunyan Fang, Qingbo Zhang, Yiguang Zhu, Liping Zhang, Wenjun Zhang, Liang Ma, Haibo Zhang, and  Changsheng Zhang.* Proximicins F and G and diproximicin A: aminofurans from the marine-derived Verrucosispora sp. SCSIO 40062 by overexpression of PPtase genes. Journal of Natural Products, 2020, 83 (4): 1152-1156. DOI:  10.1021/acs.jnatprod.9b01185.



Bin Tan,# Qingbo Zhang,# Yiguang Zhu, Hongbo Jin, Liping Zhang, Siqiang Chen, and Changsheng Zhang.* Deciphering biosynthetic enzymes leading to 4-chloro-6-methyl-5,7-dihydroxyphenylglycine, a non-proteinogenic amino acid in totopotensamides.  ACS Chemical Biology, 2020, 15 (3): 766-773. DOI: 10.1021/acschembio.9b00997.  Introducing Our Authors for Bin Tan (DOI: 10.1021/acschembio.0c00202).


Mahder S. Manenda, Marie-Ève Picard, Liping Zhang, Normand Cyr, Xiaojun Zhu, Julie Barma, Manon Couture, Changsheng Zhang, and Rong Shi.* Structural analyses of the group A flavin-dependent monooxygenase PieE reveal a sliding FAD cofactor conformation bridging OUT and IN conformations. Journal of Biological Chemistry, 2020, DOI: .


Hongbo Jin, Wenjun Zhang, Guangtao Zhang, Liping Zhang, Wei Liu, and Changsheng Zhang.* Engineered biosynthesis of 5/5/6 type polycyclic tetramate macrolactams in an ikarugamycin (5/6/5 type)-producing chassis. Organic Letters, 2020, 22 (5): 1731-1735. DOI: 10.1021/acs.orglett.9b04672.


Bin Tan,# Siqiang Chen,# Qingbo Zhang,* Yuchan Chen, Yiguang Zhu, Imran Khan, Weimin Zhang, and Changsheng Zhang.* Heterologous expression leads to discovery of diversified lobophorin analogues and a flexible glycosyltransferase. Organic Letters, 2020, 22 (3): 1062-1066. DOI: 10.1021/acs.orglett.9b04597.


2019

Liang Ma, Qingbo Zhang, Chunfang Yang, Yiguang Zhu, Liping Zhang, Lijuan Wang, Zhiwen Liu, Guangtao Zhang, and Changsheng Zhang*. Assembly line and post-PKS modifications in the biosynthesis of marine polyketide natural products. Book chapter in: Comprehensive Natural Products III: Chemistry and Biology. Book editor, Begley, T. P. & H.-w. Liu; Volume editor, Y. Tang, 2019, DOI: 10.1016/B978-0-12-409547-2.14711-0.


Wei Liu, Wenjun Zhang, Hongbo Jin, Qingbo Zhang, Yuchan Chen, Xiaodong Jiang, Guangtao Zhang, Liping Zhang, Weimin Zhang, Zhigang She, and Changsheng Zhang.* Genome mining of marine-derived Streptomyces sp. SCSIO 40010 leads to cytotoxic new polycyclic tetramate macrolactams. Marine Drugs, 2019, 17 (12): 663. DOI: 10.3390/md17120663.


Liujuan Zheng,# Xiaodong Jiang,# Qingbo Zhang, Yiguang Zhu, Haibo Zhang, Wenjun Zhang, Kumar Saurav, Jinsong Liu, and Changsheng Zhang.* Discovery and biosynthesis of neoenterocins indicate a skeleton rearrangement of enterocin. Organic Letters, 2019, 21 (22): 9066-9070. DOI: 10.1021/acs.orglett.9b03460. ("Hot off the press" in Natural Product Reprots, 2020. DOI: 10.1039/D0NP90004D


Ziquan Yu, Haibo Zhang, Chengshan Yuan, Qingbo Zhang, Imran Khan, Yiguang Zhu, and Changsheng Zhang.* Characterizing two cytochrome P450s in tiacumicin biosynthesis reveals reaction timing for tailoring modifications. Organic Letters, 2019, 21 (18): 7679-7683.  DOI: 10.1021/acs.orglett.9b03100 ("Hot off the press" in Natural Product Reprots, 2019, 36 (12): 1614-1618. DOI: 10.1039/C9NP90045D


Zhuangjie Fang, Siqiang Chen, Yiguang Zhu, Jie Li, Imran Khan, Qingbo Zhang*, and Changsheng Zhang. A new uridine derivative and a new indole derivative from the coral-associated actinomycete Pseudonocardia sp. SCSIO 11457. Natural Product Research, 2019, DOI: 10.1080/14786419.2019.1616729 .

Chunshuai Huang, Chunfang Yang, Zhaungjie Fang, Liping Zhang, Wenjun Zhang, Yiguang Zhu, and Changsheng Zhang*. Discovery of stealthin derivatives and Implication of the amidotransferase FlsN3 in the biosynthesis of nitrogen-containing fluostatins. Marine Drugs, 2019, 17 (3): 150. DOI: 10.3390/md17030150.

Chunshuai Huang, Chunfang Yang, Wenjun Zhang, Yiguang Zhu, Liang Ma, Zhuangjie Fang, and Changsheng Zhang*. Albumycin, a new isoindolequinone from Streptomyces albus J1074 harboring the fluostatin biosynthetic gene cluster. The Journal of Antibiotics, 2019, 72 (5): 311-315. DOI: 10.1038/s41429-019-0161-4.


Zhiwen Liu,# Liang Ma,# Liping Zhang, Wenjun Zhang, Yiguang Zhu, Yuchan Chen, Weimin Zhang, and Changsheng Zhang*. Functional characterization of the halogenase SpmH and discovery of new deschloro-tryptophan dimers. Organic & Biomolecular Chemistry, 2019, 17 (5): 1053-1057. DOI:  10.1039/C8OB02775G (contribution to the themed collection: "Biosynthesis" as an outside front cover. PDF)

             

2018

Huang C, Yang C, Zhu Y*, Zhang W, Yuan CS, and Zhang C*. Marine bacterial aromatic polyketides from host-dependent heterologous expression and fungal mode of cyclization. Frontiers in Chemistry, 2018, 6 (10): 528. DOI: 10.3389/fchem.2018.00528 (contribution to section Medicinal and Pharmaceutical Chemistry of research topic "Natural Products from Marine Microorganisms")

Zhang W, Zhang G, Zhang L, Liu W, Jiang X, Jin H, Liu Z, Zhang H, Zhou A, Zhang C*.  New polycyclic tetramate macrolactams from marine-derived Streptomyces sp. SCSIO 40060. Tetrahedron, 2018, 74 (47): 6839-6845. DOI: 10.1016/j.tet.2018.10.007

Huang C,# Yang C,# Zhang W, Zhang L, De BC, Zhu Y, Jiang X, Fang C, Zhang Q, Yuan CS, Liu Hw*, and Zhang C*.  Molecular basis of dimer formation during the biosynthesis of benzofluorene-containing atypical angucyclines.  Nature Communications, 2018, 9 (1): 2088. DOI: 10.1038/s41467-018-04487-z   (Featured in a Nature Communications  Editors' Highlights Webpage of recent research on Organic Chemistry and Chemical Biology by Julia Eckhoff)

Zhang H, Tian X, Pu J, Zhang Q, Zhang W, Zhang C*.  Tiacumicin congeners with improved antibacterial activities from a halogenase-inactivated mutant. Journal of Natural Products, 2018, 81 (5): 1219–1224. DOI: 10.1021/acs.jnatprod.7b00990

Lin W#, Das K#*, Degen D#, Mazumder A, Duchi D, Wang D, Ebright YW, Ebright RY, Sineva E, Gigliotti M, Mandal S, Jiang Y, Liu Y, Yin R, Zhang Z, Eng ET, Thomas D, Donadio S, Zhang H, Zhang C, Kapanidis AN, and Ebright RH*. Structural basis of transcription inhibition by fidaxomicin (lipiarmycin A3). Molecular Cell, 2018, 70 (1): 60-71. DOI: 10.1016/j.molcel.2018.02.026

2017

Chen R#, Zhang Q#, Tan B, Zheng L, Li H, Zhu Y, Zhang C*. Genome mining and activation of a silent PKS/NRPS gene cluster direct the production of totopotensamides. Organic Letters, 2017, 19(20): 5697-5700. DOI: 10.1021/acs.orglett.7b02878

Ma L, Zhang W, Zhu Y, Zhang G, Zhang H, Zhang L, Zhang Q, Yuan C, Zhang C*. Identification and characterization of a gene cluster for tryptophan dimers in deep sea-derivedStreptomyces sp. SCSIO 03032. Applied Microbiology and Biotechnology, 2017, 101 (15): 6123–6136. DOI: 10.1007/s00253-017-8375-5

Zhang Q,# Li H,#  Yu L, Sun Y, Zhu Y, Zhu H, Zhang L, Li SM, Shen Y, Tian C, Li A, Liu Hw,* Zhang C*. Characterization of the flavoenzyme XiaK as an N-hydroxylase and implications in indolosesquiterpene diversification. Chemical Science, 2017, 8(7): 5067-5077. DOI: 10.1039/C7SC01182B. ("Hot off the press" in Natural Product Reports, 2017, 34 (10): 1180-1184. DOI: 10.1039/C7NP90040F; Selected in Themed collection "In celebration of Chinese New Year", a special online collection of articles to celebrate Chinese New Year. This collection includes some of most popular articles from China and highlights a number of outstanding contributions)


Jiang X, Zhang Q, Zhu Y, Nie F, Wu Z, Yang C, Zhang L, Tian X, Zhang C*. Isolation, structure elucidation and biosynthesis of benzo[b]fluorene nenestatin A from deep-sea derived Micromonospora echinospora SCSIO 04089. Tetrahedron, 2017, 73 (26): 3585–3590(Dedicated to the Tetrahedron Young Investigator Prize Special Issue in honor of Professor Ang Li). DOI: 10.1016/j.tet.2017.03.054

Zhang W#, Yang C#, Huang C, Zhang L, Zhang H, Zhang Q, Zhu Y, Zhang C*. Pyrazolofluostatins A-C,  pyrazole-fused nenzo[a]fluorenes from South China Sea-derived Micromonospora rosaria SCSIO N160. Organic Letters, 2017, 19 (3): 592–595. ("Hot off the press" in Natural Product Reports, 2017, 34 (4) : 338-342). DOI: 10.1021/acs.orglett.6b03745


Saha S#, Zhang W#, Zhang G#, Zhu Y, Chen Y, Liu W, Yuan CS, Zhang Q, Zhang H, Zhang L, Zhang W, Zhang C*. Activation and characterization of a cryptic gene cluster reveal a cyclization cascade for polycyclic tetramate macrolactams. Chemical Science, 2017,8 (2): 1607-1612. DOI: 10.1039/C6SC03875A

2016

Zhu Y#, Picard MÈ#, Zhang Q, Barma J, Després X, Mei X, Zhang L, Duvignaud J, Couture M, Zhu W, Shi R*, Zhang C*.  Flavoenzyme CrmK-mediated substrate recycling in caerulomycin biosynthesisChemical Science, 2016,7(8): 4867-4874. DOI: 10.1039/c6sc00771f

Graphical abstract: Flavoenzyme CrmK-mediated substrate recycling in caerulomycin biosynthesis


Zhu Y#, Xu J#, Mei X#, Feng Z, Zhang L, Zhang Q, Zhang G, Zhu W*, Liu J*, Zhang C*. Biochemical and structural insights into the aminotransferase CrmG in caerulomycin biosynthesis. ACS Chemical Biology, 2016,11 (4): 943–952. DOI: 10.1021/acschembio.5b00984

Zhang H#, Saurav K#, Yu Z, Kurtán T, Mándi A, Li J, Tian X, Zhang Q, Zhang W, Zhang C*. α-Pyrones with diverse hydroxy substitutions from three marine-derived Nocardiopsis strains. Journal of Natural Products, 2016, 79(6): 1610–1618. DOI: 10.1021/acs.jnatprod.6b00175

Zhang G, Zhang W, Saha S, Zhang C*. Recent advances in discovery, biosynthesis and genome mining of medicinally relevant polycyclic tetramate macrolactams. Current Topics in Medicinal Chemistry, 2016, 16(15):1727-1739. (invited review). DOI: 10.2174/1568026616666151012112818


Tian X, Zhang W, Yang T, Chen M, Li J, Chen F, Yang J, Li W, Zhang B, Zhang Z, Wu J, Zhang C, Long L*, Xiao J*. Comparative genomics analysis ofStreptomycesspecies reveals their adaptation to the marine environment and their diversity at the genomic level. Frontiers in Microbiology, 2016, http://dx.doi.org/10.3389/fmicb.2016.00998.


2015


Yang C, Huang C, Zhang W*, Zhu Y, Zhang C*. Heterologous expression of fluostatin gene cluster leads to a bioactive heterodimer. Organic Letters, 2015, 17(21): 5324-5327. DOI:  10.1021/acs.orglett.5b02683 ("Hot off the press" in Natural Product Reports, 2016, 33(2): 122-126)

Zhu Y#, Zhang W#, Chen Y, Yuan CS, Zhang H, Zhang G, Ma L, Zhang Q, Tian X, Zhang S, Zhang C*. Characterization of heronamide biosynthesis reveals a tailoring hydroxylase and indicates migrated double bonds. ChemBioChem, 2015, 16(14): 2086-2093. DOI: 10.1002/cbic.201500281

Li H#, Sun Y#, Zhang Q, Zhu Y, Li SM, Li A, Zhang C*. Elucidating the cyclization cascades in xiamycin biosynthesis by substrate synthesis and enzyme characterizations. Organic Letters, 2015, 17(2): 306-309. DOI: 10.1021/ol503399b ("Hot off the press" in Natural Product Reports, 2015, 32(4): 512-516)

Medema MH*, Kottmann R, Yilmaz P, Cummings M, Biggins JB, Blin K, de Bruijn I, Chooi YH, Claesen J, Coates RC, Cruz-Morales P, Duddela S, Düsterhus S, Edwards DJ, Fewer DP, Garg N, Geiger C, Gomez-Escribano JP, Greule A, Hadjithomas M, Haines AS, Helfrich EJ, Hillwig ML, Ishida K, Jones AC, Jones CS, Jungmann K, Kegler C, Kim HU, Kötter P, Krug D, Masschelein J, Melnik AV, Mantovani SM, Monroe EA, Moore M, Moss N, Nützmann HW, Pan G, Pati A, Petras D, Reen FJ, Rosconi F, Rui Z, Tian Z, Tobias NJ, Tsunematsu Y, Wiemann P, Wyckoff E, Yan X, Yim G, Yu F, Xie Y, Aigle B, Apel AK, Balibar CJ, Balskus EP, Barona-Gómez F, Bechthold A, Bode HB, Borriss R, Brady SF, Brakhage AA, Caffrey P, Cheng YQ, Clardy J, Cox RJ, De Mot R, Donadio S, Donia MS, van der Donk WA, Dorrestein PC, Doyle S, Driessen AJ, Ehling-Schulz M, Entian KD, Fischbach MA, Gerwick L, Gerwick WH, Gross H, Gust B, Hertweck C, Höfte M, Jensen SE, Ju J, Katz L, Kaysser L, Klassen JL, Keller NP, Kormanec J, Kuipers OP, Kuzuyama T, Kyrpides NC, Kwon HJ, Lautru S, Lavigne R, Lee CY, Linquan B, Liu X, Liu W, Luzhetskyy A, Mahmud T, Mast Y, Méndez C, Metsä-Ketelä M, Micklefield J, Mitchell DA, Moore BS, Moreira LM, Müller R, Neilan BA, Nett M, Nielsen J, O'Gara F, Oikawa H, Osbourn A, Osburne MS, Ostash B, Payne SM, Pernodet JL, Petricek M, Piel J, Ploux O, Raaijmakers JM, Salas JA, Schmitt EK, Scott B, Seipke RF, Shen B, Sherman DH, Sivonen K, Smanski MJ, Sosio M, Stegmann E, Süssmuth RD, Tahlan K, Thomas CM, Tang Y, Truman AW, Viaud M, Walton JD, Walsh CT, Weber T, van Wezel GP, Wilkinson B, Willey JM, Wohlleben W, Wright GD, Ziemert N, Zhang C, Zotchev SB, Breitling R, Takano E, Glöckner FO. Minimum information about a biosynthetic gene cluster. Nature Chemical Biology, 2015, 11(9): 625-631. DOI:  10.1038/nchembio.1890

2014


Zhang G#, Zhang W#, Zhang Q, Shi T, Ma L, Zhu Y, Li S, Zhang H, Zhao YL, Shi R, Zhang C*. Mechanistic Insights Into Polycycle Formation by Reductive Cyclization in Ikarugamycin Biosynthesis. Angewandte Chemie International Edition, 2014, 53(19): 4840-4844. (Recommended by Faculty of 1000; "Hot off the press" in Natural Product Reports, 2014, 31(9): 1083-1087).


Chen Y#, Zhang W#, Zhu Y*, Zhang Q, Tian X, Zhang S, Zhang C*. Elucidating Hydroxylation and Methylation Steps Tailoring Piericidin A1 Biosynthesis. Organic Letters, 2014, 16(3): 736-739.

Fu P#, Zhu Y#, Mei X, Wang Y, Jia H, Zhang C*, Zhu W*. Acyclic Congeners fromActinoalloteichus cyanogriseusProvide Insights into Cyclic Bipyridine Glycoside Formation. Organic Letters, 2014, 16(16): 4264-4267.

Zhang W, Ma L, Li S, Liu Z, Chen Y, Zhang H, Zhang G, Zhang Q, Tian X, Yuan C, Zhang S, Zhang WM, Zhang C*. Indimicins A–E, Bisindole Alkaloids from the Deep Sea-DerivedStreptomycessp. SCSIO 03032. Journal of Natural Products, 2014, 77(8): 1887-1892. (Featured as "Key Drug Discovery Article" by Global Medical Discovery)

Zhang W, Li S, Zhu Y, Chen YC, Chen YL, Zhang HB, Zhang G, Tian X, Pan Y, Zhang S, Zhang WM, Zhang C*. Heronamides D–F, Polyketide Macrolactams from the Deep-Sea DerivedStreptomycessp. SCSIO 03032. Journal of Natural Products, 2014, 77(2): 388–391.

2013


Zhu Y, Zhang Q, Li S, Lin Q, Fu P, Zhang G, Zhang H, Shi R, Zhu W*, Zhang C*. Insights into Caerulomycin A Biosynthesis: A Two-component Monooxygenase CrmH-Catalyzed Oxime Formation. Journal of the American Chemical Society, 2013, 135(50): 18750-18753. ("Hot off the press" inNatural Products Reports, 2014, 31(4): 414-418).

Chen R#, Zhang H#, Zhang G, Li S, Zhang G, Zhu Y, Liu J, Zhang C*. Characterizing Amosamine Biosynthesis in Amicetin Reveals AmiG as a Reversible Retaining GlycosyltransferaseJournal of the American Chemical Society, 2013, 135 (33): 12152–12155.



Li S#, Xiao J#, Zhu Y, Zhang G, Yang C, Zhang H, Ma L, Zhang C*.Dissecting Glycosylation Steps in Lobophorin Biosynthesis Implies an Iterative Glycosyltransferase. Organic Letters, 2013, 15 (6): 1374-1377.


Wu Z, Li S, Li J, Chen Y, Saurav K, Zhang Q, Zhang H, Zhang W, Zhang W, Zhang S, Zhang C*. Antibacterial and Cytotoxic New Napyradiomycins from the Marine-Derived Streptomyces sp. SCSIO 10428. Marine Drugs, 2013, 11(6): 2113-2125.



Xiao J, Zhang Q, Zhu Y, Li S, Zhang G, Zhang H, Saurav K, Zhang C*. Characterization of the Sugar-O-Methyltransferase LobS1 in Lobophorin Biosynthesis. Applied Microbiology and Biotechnology, 2013, 97(20): 9043-9053.




Wu ZC, Li S, Nam SJ, Liu Z, Zhang C*. Nocardiamides A and B, Two Cyclohexapeptides from the Marine-Derived Actinomycete Nocardiopsis sp. CNX037. Journal of Natural Products, 2013, 76 (4): 694–701. DOI: 10.1021/np400009a


Zhang Q, Li S, Chen Y, Tian X, Zhang H, Zhang G, Zhu Y, Zhang S, Zhang W, Zhang C*. New Diketopiperazine Derivatives from a Deep-Sea-Derived Nocardiopsis alba SCSIO 03039. The Journal of Antibiotics, 2013, 66 (1): 31-36.



Tian XP, Long LJ, Li SM, Zhang J, Xu Y, He J, Li J, Wang FZ, Li WJ, Zhang C, Zhang S*. Pseudonocardia antitumoralis sp. nov., a new deoxynyboquinone-producing actinomycete isolated from a deep-sea sedimental sample in South China Sea. International Journal of Systematic and Evolutionary Microbiology, 2013, 63 (Pt 3):893-899.

2012


Li H#, Zhang Q#, Li S, Zhu Y, Zhang G, Zhang H, Tian X, Zhang S, Ju J, Zhang C*. Identification and Characterization of Xiamycin A and Oxiamycin Gene Cluster Reveals an Oxidative Cyclization Strategy Tailoring Indolosesquiterpene Biosynthesis. Journal of the American Chemical Society, 2012, 134 (21): 8996-9005.

Zhu Y#, Fu P#, Lin Q, Zhang G, Zhang H, Li S, Ju J, Zhu W*, Zhang C*. Identification of Caerulomycin A Gene Cluster Implicates a Tailoring Amidohydrolase. Organic Letters, 2012, 14 (11): 2666-2669. ("Hot off the press" inNatural Product Reports, 2012, 29: 829-833)

Zhang W, Liu Z, Li S, Yang T, Zhang Q, Ma L, Tian X, Zhang H, Huang C, Zhang S, Ju J, Shen Y, Zhang C*. Spiroindimicins A–D: New Bisindole Alkaloids from a Deep-Sea Derived Actinomycete. Organic Letters, 2012, 14(13): 3364-3367. ("Hot off the press" inNatural Product Reports, 2012, 29: 1033-1037)

Zhang Q#, Li H#, Li S, Zhu Y, Zhang G, Zhang H, Zhang W, Shi R, Zhang C*. Carboxyl Formation from Methyl via Triple Hydroxylations by XiaM in Xiamycin A Biosynthesis. Organic Letters, 2012, 14 (24): 6142–6145. ("Hot off the press" inNatural Product Reports, 2013, 30: 485-489)

Zhang G#, Zhang H#, Li S, Xiao J, Zhang G, Zhu Y, Niu S, Ju J, Zhang C*. Characterization of the Amicetin Biosynthetic Gene Cluster from Streptomyces vinaceusdrappus NRRL 2363 Implicates Two Alternative Strategies for Amide Bond Formation. Applied and Environmental Microbiology, 2012, 78(7): 2393-2401.



Zhang Q, Mándi A, Li S, Tian X, Zhang W, Chen Y, Zhang H, Li H, Zhang WM, Zhang S, Ju J, Kurtán T*, Zhang C*. N–N-coupled indolosesquiterpene atropo-diastereomers from a marine-derived actinomycete. European Journal of Organic Chemistry, 2012, 2012(27): 5256-5262.


Zhang W#, Liu Z#, Li S, Lu Y, Chen Y, Zhang H, Zhang G, Zhu Y, Zhang G, Zhang W, Liu J, Zhang C*. Fluostatins I-K from the South China Sea-Derived Micromonospora rosaria SCSIO N160. Journal of Natural Products, 2012, 75(11): 1937–1943.



Tian XP, Xu Y, Zhang J, Li J, Chen Z, Kim CJ, Li WJ, Zhang C, Zhang S*. Streptomyces oceanisp. nov., a new obligate marine actinomycete isolated from a deep-sea sample of seep authigenic carbonate nodule in South China Sea. Antonie Van Leeuwenhoek, 2012 , 102(2): 335-343.

Li J, Zhang GT, Yang J, Tian XP, Wang FZ, Zhang C, Zhang S*, Li WJ*. Marininema mesophilumgen. nov., sp. nov., a thermoactinomycete isolated from deep sea sediment, and emended description of the family Thermoactinomycetaceae. International Journal of Systematic and Evolutionary Microbiology, 2012, 62(Pt 6):1383-1388.

Tian XP, Long LJ, Wang FZ, Xu Y, Li J, Zhang J, Zhang C, Zhang S*, Li WJ*. Streptomyces nanhaiensissp. nov., a novel marine streptomycete isolated from a deep sea sediment in Southern China Sea. International Journal of Systematic and Evolutionary Microbiology, 2012, 62(Pt 4): 864-868.


2011


Xiao Y#, Li S#, Niu S, Ma L, Zhang G, Zhang H, Zhang GY, Ju J, Zhang C*. Characterization of Tiacumicin B Biosynthetic Gene Cluster Affording Diversified Tiacumicin Analogues and Revealing a Tailoring Dihalogenase.  Journal of the American Chemical Society, 2011, 133 (4): 1092–1105. ("Hot off the press" inNatural Product Reports, 2011, 28: 659-662)

Niu S#, Hu T#, Li S, Xiao Y, Ma L, Zhang G. Zhang H, Zhang S, Yang X, Zhang C*. Characterization of a Sugar-O-methyltransferase TiaS5 Affords New Tiacumicin Analogues with Improved Antibacterial Property and Reveals Substrate Promiscuity. ChemBioChem, 2011, 12(11): 1740-1748.

Li S, Tian XP, Niu S, Zhang W, Chen Y, Zhang H, Zhang W, Li WJ, Zhang S, Ju J, Zhang C*. Pseudonocardians A-C, New Diazaanthraquinone Derivatives from a Deep-Sea Actinomycete Pseudonocardia sp. SCSIO 01299. Marine Drugs, 2011, 2011, 9(8): 1428-1439.



Niu S, Li S, Chen Y, Tian X, Zhang H, Zhang G, Zhang W, Yang X, Zhang S, Ju J, Zhang C*. Lobophorins E and F, new spirotetronate antibiotics from a South China Sea-derivedStreptomycessp. SCSIO 01127. The Journal of Antibiotics, 2011, 64(11):711-716.



Gong L, Xiao Y, Liu Q, Li S, Zhang C*, Liu J*. Preliminary X-ray crystallographic analysis of the glycosyltransferase from a marine Streptomyces species. Acta Crystallogr Sect F Struct Biol Cryst Commun., 2011, 67(1): 136-139.


Postdoctoral and graduate:


Williams GJ, Yang J, Zhang C, Thorson JS*. Recombinant E. coli Prototype Strains for in Vivo Glycorandomization.ACS Chemical Biology, 2011, 6(1): 94-100.



Zhang C, Griffith BR, Fu Q, Albermann C, Fu X, Lee IK, Li L, Thorson JS*.Exploiting the Reversibility of Natural Product Glycosyltransferase-catalyzed Reactions.Science, 2006, 313(5791): 1291-1294. (Featured inNature, 2006, 443: 285;ACS Chemical Biology, 2006, 1, 499;Angewandte Chemie International Edition, 2007, 46:2147; highlighted inNature, 2006, 443: 5 andNature Chemical Biology, 2006, 2: 517;Nature Methods, 2006, 3: 873 andChemical & Engineering News, 2006, 84:13; and reported in various press includingUnited Press International,Wisconsin State Journal,Wisconsin Technology Network,UW news,The Daily Cardinal,Milwaukee Journal Sentinel, etc.)



Zhang C, Jiang J, Morreti R, Thorson JS*. The In Vitro Characterization of Polyene Glycosyltransferases AmphDI and NysDI.ChemBioChem, 2008, 9(15): 2506-2514.

Zhang C, Bitto E, Goff RD, Griffith BR, Albermann C, Bingman CA, Phillips Jr GN*, Thorson JS*. Biochemical and Structural Insights of the Early Glycosylation Steps in Calicheamicin Biosynthesis.Chemistry and Biology, 2008, 15(8): 842-853.



Zhang C, Fu Q, Albermann C, Li L, Thorson JS*. The In Vitro Characterization of the Erythronolide Mycarosyltransferase EryBV and Its Utility in Macrolide Diversification.ChemBioChem, 2007, 8(4): 385-390.

Zhang C, Albermann C, Fu X, Thorson JS*. The In Vitro Characterization of the Iterative Avermectin Glycosyltransferase AveBI Reveals Reaction Reversibility and Sugar Nucleotide Flexibility.  Journal of the American Chemical Society, 2006, 128(51): 16420-16421.

Zhang C, Albermann C, Fu X, Peters NR, Chisholm JD, Zhang G, Gilbert EJ, Wang PG, Van Vranken DL, Thorson JS*. RebG- and RebM-catalyzed Indolocarbazole Diversification.ChemBioChem, 2006, 7(5): 795-804.

Zhang C, Weller RL, Thorson JS, Rajski SR*. Natural Product Diversification Using Non-natural Cofactor Analogues of S-adenosyl-L-methionine.  Journal of the American Chemical Society, 2006, 128(9): 2760-2761.

Zhang C, Podeschwa M, Block O, Altenbach HJ, Piepersberg W, Wehmeier UF*. Identification of a 1-epi-valienol 7-kinase activity in the producer of acarbose,Actinoplanessp. SE50/110.FEBS Letters, 2003, 540(1-3): 53-57.

Zhang C, Podeschwa M, Altenbach HJ, Piepersberg W and Wehmeier UF*. The acarbose-biosynthetic enzyme AcbO fromActinoplanessp. SE 50/110 is a 2-epi-5-epi-valiolone-7-phosphate 2-epimerase.FEBS Letters, 2003, 540(1-3): 47-52.

Zhang C, Stratmann A, Block O, Brückner R, Podeschwa M, Altenbach HJ, Wehmeier UF and Piepersberg W*. Biosynthesis of the C7-cyclitol Moiety of Acarbose in Actinoplanes Species SE50/110. 7-O-phosphorylation of the initial cyclitol precursor leads to proposal of a new biosynthetic pathway.Journal of Biological Chemistry, 2002, 277(25): 22853-22862.



Williams GJ,Zhang C, Thorson JS*. Expanding the Promiscuity of A Natural Product Glycosyltransferase by Directed Evolution.Nature Chemical Biology, 2007, 3(10): 657-662. (Featured inNature Chemical Biology, 2007, 3: 604; andChemical & Engineering News, 2007, 85: 9; and reported in various press including:United Press International,Chemistry World,UW news, andWisconsin State Journal, etc.)



Borisova SA,Zhang C, Takahashi H, Zhang H, Wong AW, Thorson JS, Liu H-w*. Substrate Specificity of the Macrolide-Glycosylating Enzyme Pair DesVII/DesVIII: Opportunities, Limitations and Mechanistic Hypotheses.Angewandte Chemie International Edition, 2006, 45(17): 2748-2753.

Gao Q,Zhang C, Blanchard S, Thorson JS*. Deciphering indolocarbazole and enediyne aminopentose biosynthesis through comparative genomics: New insights and tools from theActinomadura melliauraAT2433 biosynthetic locus.Chemistry and Biology, 2006, 13(7):733-743.



Williams GJ, Goff RD,Zhang C, Thorson JS*. Optimizing glycosyltransferase specificity via ‘hot spot’ saturation mutagenesis presents a new catalyst for novobiocin glycorandomization.Chemistry and Biology, 2008, 15(4): 393-401.



Singh S, McCoy JG,Zhang C, Bingman CA, Phillips Jr GN*, Thorson JS*. Structure and Mechanism of the Rebeccamycin Sugar 4-O-Methyltransferase RebM.Journal of Biological Chemistry, 2008, 283(33): 22628-22636.



Singh S, Hager MH,Zhang C, Griffith BR, Lee MS, Hallenga K, Markley JL and Thorson JS*. Structural insight into self-sacrifice mechanism of enediyne resistance.ACS Chemical Biology, 2006, 1(7): 451-460.

Fu X, Albermann C,Zhang C, Thorson JS*. Diversifying Vancomycin via Chemoenzymatic Strategies.Organic Letters, 2005, 7(8): 1513-1515.

Fu X, Albermann C, Jiang J, Liao J,Zhang C, Thorson JS*. Antibiotic optimization via in vitro glycorandomization.Nature Biotechnology, 2003, 21(12): 1467-1469.







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