张长生 男 博导 南海海洋研究所
电子邮件:czhang@scsio.ac.cn;czhang2006@gmail.com
通信地址:广州市新港西路164号
邮政编码:510301

研究领域和科研项目

研究领域

海洋微生物的分离鉴定、活性次生代谢产物的天然发现、生物合成和结构改造等方面的研究工作,探索结构新颖、活性独特的微生物次生代谢产物的生物合成途径和所蕴藏的新颖生物合成酶反应机制及其在天然药物结构多样化方面的应用。

1. 微生物天然活性物质的分离鉴定。采用微生物学和化学手段,从不同环境(主要是特殊环境的海洋生态系统,包括海底沉积物、珊瑚礁和海绵)中筛选分离特殊的微生物(主要包括放线菌和真菌),并通过发酵和活性筛选从微生物中分离和鉴定具有生物活性的小分子化合物或者大分子复合物。

2. 复杂微生物次生代谢产物的生物合成研究。用分子生物学手段克隆完整的生物合成基因簇,研究生物合成途径,进行基因遗传操作(包括基因阻断、置换和重组等),用组合生物合成的手段创造“非天然”的天然化合物,筛选新的生物活性。课题组已展开了针对新型抗菌素和抗肿瘤药物如友菌素(amicetin)、台勾霉素(tiacumicin)、浅蓝霉素(caerulomicin)、Elaiophylin、lobophorins、DNQ和斑鸠霉素(ikarugamycin)等多种抗生素的生物合成工作。

3.微生物次生代谢产物新功能酶的作用机制及其应用。用生物化学手段来进行体外研究并揭示复杂天然产物生物合成中所包含的独特酶学机制,开发这些酶(如糖基转移酶,甲基化酶,氧化还原酶等)在天然产物结构多样化方面的应用潜力。


科研项目

国家自然科学基金委

              杰出青年基金 2012-2015

              重点项目 2017-2021

              重点国际(地区)合作研究项目 2019-2023

              面上项目(3)

国家重点基础研究发展计划(973计划)课题  2010-2014

中科院前沿领域重点项目 2016-2021

重要方向性项目 2009-2011

中科院先导专项重点任务 2013-2017

广东省海洋经济创新发展区域示范项目,课题 2013-2017

广东省自然基金重大培育项目 2015-2018

广东省特支计划人才项目(2) 2015-2019

广东省渔港建设和渔业产业发展专项 2016-2018

招生信息

   
招生专业
070703-海洋生物学
070321-化学生物学
071005-微生物学
招生方向
海洋生物学
海洋天然产物化学
海洋微生物学

教育背景

1998-09--今 德国乌帕塔尔大学 博士
1994-09--今 华东理工大学 硕士
1990-08--今 上海交通大学 学士

工作经历

2015/04  --  今       中国科学院南海海洋研究所,所长助理

2012/08  --  今       中国科学院南海海洋研究所,科研与规划处处长

2008/03  --  今       中国科学院南海海洋研究所, 研究员

2003/01  --  2008/03  美国威斯康星大学药学院博士后,assistant Scientist

1997/07  --  1998/08 上海太平洋生物高科技有限公司,助理研究员       

教授课程

海洋生物资源可持续利用讲座

获得荣誉

2018年:  2018年度"中国科学院优秀导师奖"

2018年:  2018年度中国科学院广州教育基地"优秀研究生导师"

2017年:  享受“国务院政府特殊津贴”,中华人民共和国国务院

2016年:   国家“万人计划”领军人才,中央人才工作协调小组办公室

2016年:   第四届曾呈奎海洋科技奖--青年科技奖,中国海洋湖沼学会

2015年:  广东特支计划“杰出人才(南粤百杰)”,广东省人力资源和社会保障厅

2014年:  国家百千万人才工程”有突出贡献中青年专家“,国家人力资源社会保障部

2014年:  中青年科技创新领军人才,国家科学与技术部

2014年:  广东特支计划”百千万工程领军人才”,广东省人力资源和社会保障厅

2013年:“百人计划“终期评估”优秀”,中国科学院

2012年:   全国优秀科技工作者,中国科协

2011年:   国家杰出青年基金获得者(终期评估优秀),国家自然科学基金委员会

2009年:“百人计划”择优支持入选者,中国科学院 

2005年: “First Midwest Carbohydrate Symposium”, Anatrace Best Poster Award  

出版信息

# (co-first author), * (corresponding author)

2019

2019

Huang C, Yang C, Fang Z, Zhang L, Zhang W, Zhu Y, Zhang C*. Discovery of stealthin derivatives and Implication of the amidotransferase FlsN3 in the biosynthesis of nitrogen-containing fluostatins. Marine Drugs, 2019, DOI: 10.3390/md17030150.

Huang C, Yang C, Zhang W, Zhu Y, Ma L, Fang Z, and Zhang C*. Albumycin, a new isoindolequinone from Streptomyces albus J1074 harboring the fluostatin biosynthetic gene cluster. The Journal of Antibiotics, 2019,DOI: 10.1038/s41429-019-0161-4.

Liu Z,# Ma L,# Zhang L, Zhang W, Zhu Y, Chen Y, Zhang W, Zhang C.* Functional characterization of the halogenase SpmH and discovery of new deschloro-tryptophan dimers. Organic & Biomolecular Chemistry, 2019, 17 (5): 1053-1057. DOI:  10.1039/C8OB02775G (contribution to the themed collection: "Biosynthesis" as an outside front cover. PDF)

             



2018

Huang C, Yang C, Zhu Y*, Zhang W, Yuan CS, and Zhang C*. Marine bacterial aromatic polyketides from host-dependent heterologous expression and fungal mode of cyclization. Frontiers in Chemistry, 2018, 6(10): 528, DOI: 10.3389/fchem.2018.00528 (contribution to section Medicinal and Pharmaceutical Chemistry of research topic "Natural Products from Marine Microorganisms")

Zhang W, Zhang G, Zhang L, Liu W, Jiang X, Jin H, Liu Z, Zhang H, Zhou A, Zhang C*.  New polycyclic tetramate macrolactams from marine-derived Streptomyces sp. SCSIO 40060. Tetrahedron, 2018, 74 (47): 6839-6845. DOI: 10.1016/j.tet.2018.10.007

Huang C,# Yang C,# Zhang W, Zhang L, De BC, Zhu Y, Jiang X, Fang C, Zhang Q, Yuan CS, Liu Hw*, and Zhang C*.  Molecular Basis of Dimer Formation during the Biosynthesis of Benzofluorene-Containing Atypical Angucyclines.  Nature Communications, 2018, 9 (1): 2088. DOI: 10.1038/s41467-018-04487-z   (Featured in a Nature Communications Editors' Highlights Webpage of recent research on Organic Chemistry and Chemical Biology by Julia Eckhoff. https://www.nature.com/collections/wdzvyhgxft)

Zhang H, Tian X, Pu J, Zhang Q, Zhang W, Zhang C*.  Tiacumicin Congeners with Improved Antibacterial Activities from a Halogenase-Inactivated Mutant. Journal of Natural Products, 2018, 81 (5): 1219–1224. DOI: 10.1021/acs.jnatprod.7b00990

Lin W#, Das K#*, Degen D#, Mazumder A, Duchi D, Wang D, Ebright YW, Ebright RY, Sineva E, Gigliotti M, Mandal S, Jiang Y, Liu Y, Yin R, Zhang Z, Eng ET, Thomas D, Donadio S, Zhang H, Zhang C, Kapanidis AN, and Ebright RH*. Structural basis of transcription inhibition by fidaxomicin (lipiarmycin A3). Molecular Cell, 2018, 70(1): 60-71. DOI: 10.1016/j.molcel.2018.02.026


2017

Chen R#, Zhang Q#, Tan B, Zheng L, Li H, Zhu Y, Zhang C*. Genome Mining and Activation of a Silent PKS/NRPS Gene Cluster Direct the Production of Totopotensamides. Organic Letters, 2017, 19(20): 5697-5700. DOI: 10.1021/acs.orglett.7b02878

Ma L, Zhang W, Zhu Y, Zhang G, Zhang H, Zhang L, Zhang Q, Yuan C, Zhang C*. Identification and Characterization of a Gene Cluster for Tryptophan Dimers in Deep Sea-DerivedStreptomycessp. SCSIO 03032. Applied Microbiology and Biotechnology, 2017, 101 (15): 6123–6136. DOI: 10.1007/s00253-017-8375-5

Zhang Q,# Li H,#  Yu L, Sun Y, Zhu Y, Zhu H, Zhang L, Li SM, Shen Y, Tian C, Li A, Liu Hw,* Zhang C*. Characterization of the Flavoenzyme XiaK as an N-Hydroxylase and Implications in Indolosesquiterpene Diversification. Chemical Science, 2017, 8(7): 5067-5077. DOI: 10.1039/C7SC01182B. ("Hot off the press" in Natural Product Reports, 2017, 34 (10): 1180-1184. DOI: 10.1039/C7NP90040F)


Jiang X, Zhang Q, Zhu Y, Nie F, Wu Z, Yang C, Zhang L, Tian X, Zhang C*. Isolation, structure elucidation and biosynthesis of benzo[b]fluorene nenestatin A from deep-sea derived Micromonospora echinospora SCSIO 04089. Tetrahedron, 2017, 73 (26): 3585–3590(Dedicated to the Tetrahedron Young Investigator Prize Special Issue in honor of Professor Ang Li). DOI: 10.1016/j.tet.2017.03.054

Zhang W#, Yang C#, Huang C, Zhang L, Zhang H, Zhang Q, Zhu Y, Zhang C*. Pyrazolofluostatins A-C,  Pyrazole-fused Benzo[a]fluorenes from South China Sea-derived Micromonospora rosaria SCSIO N160. Organic Letters, 2017, 19 (3): 592–595. ("Hot off the press" in Natural Product Reports, 2017, 34 (4) : 338-342). DOI: 10.1021/acs.orglett.6b03745


Saha S#, Zhang W#, Zhang G#, Zhu Y, Chen Y, Liu W, Yuan CS, Zhang Q, Zhang H, Zhang L, Zhang W, Zhang C*. Activation and Characterization of a Cryptic Gene Cluster Reveal a Cyclization Cascade for Polycyclic Tetramate Macrolactams. Chemical Science, 2017,8 (2): 1607-1612. DOI: 10.1039/C6SC03875A

2016

Zhang H#, Saurav K#, Yu Z, Tibor K, Mándi A, Li J, Tian X, Zhang Q, Zhang W, Zhang C*. α-Pyrones with Diverse Hydroxy Substitutions from Three Marine-Derived Nocardiopsis Strains. Journal of Natural Products, 2016,  79(6): 1610-1618.


Zhu Y#, Picard MÈ#, Zhang Q, Barma J, Després X, Mei X, Zhang L, Duvignaud J, Couture M, Zhu W, Shi R*, Zhang C*. Flavoenzyme CrmK-Mediated Substrate Recycling in Caerulomycin BiosynthesisChemical Science, 2016, DOI: 10.1039/C6SC00771F.

Graphical abstract: Flavoenzyme CrmK-mediated substrate recycling in caerulomycin biosynthesis


Zhu Y#, Xu J#, Mei X#, Feng Z, Zhang L, Zhang Q, Zhang G, Zhu W*, Liu J*, Zhang C*. Biochemical and Structural Insights into the Aminotransferase CrmG in Caerulomycin Biosynthesis. ACS Chemical Biology, 2016, 11(4): 943–952.


Zhang G, Zhang W, Saha S, Zhang C*. Recent Advances in Discovery, Biosynthesis and Genome Mining of Medicinally Relevant Polycyclic Tetramate Macrolactams. Current Topics in Medicinal Chemistry, 2016, 16(15):1727-1739. (invited review)



Tian X, Zhang W, Yang T, Chen M, Li J, Chen F, Yang J, Li W, Zhang B, Zhang Z, Wu J, Zhang C, Long L*, Xiao J*. Comparative genomics analysis of Streptomyces species reveals their adaptation to the marine environment and their diversity at the genomic level. Frontiers in Microbiology, 2016, http://dx.doi.org/10.3389/fmicb.2016.00998.


2015


Yang C, Huang C, Zhang W*, Zhu Y,Zhang C*. Heterologous Expression of Fluostatin Gene Cluster Leads to a Bioactive Heterodimer. Organic Letters, 2015, 17(21): 5324-5327. (
"Hot off the press" inNatural Product Reports, 2016,33(2): 122-126).



Zhu Y#, Zhang W#, Chen Y, Yuan CS, Zhang H, Zhang G, Ma L, Zhang Q, Tian X, Zhang S,Zhang C*. Characterization of Heronamide Biosynthesis Reveals A Tailoring Hydroxylase and Indicates Migrated Double Bonds. ChemBioChem, 2015, 16(14): 2086-2093.



Li H#, Sun Y#, Zhang Q, Zhu Y, Li SM, Li A,Zhang C*. Elucidating the Cyclization Cascades in Xiamycin Biosynthesis by Substrate Synthesis and Enzyme Characterizations. Organic Letters,2015, 17(2): 306-309. ("Hot off the press" inNatural Product Reports, 2015, 32(4): 512-516).



Medema MH*, Kottmann R, Yilmaz P, Cummings M, Biggins JB, Blin K, de Bruijn I, Chooi YH, Claesen J, Coates RC, Cruz-Morales P, Duddela S, Düsterhus S, Edwards DJ, Fewer DP, Garg N, Geiger C, Gomez-Escribano JP, Greule A, Hadjithomas M, Haines AS, Helfrich EJ, Hillwig ML, Ishida K, Jones AC, Jones CS, Jungmann K, Kegler C, Kim HU, Kötter P, Krug D, Masschelein J, Melnik AV, Mantovani SM, Monroe EA, Moore M, Moss N, Nützmann HW, Pan G, Pati A, Petras D, Reen FJ, Rosconi F, Rui Z, Tian Z, Tobias NJ, Tsunematsu Y, Wiemann P, Wyckoff E, Yan X, Yim G, Yu F, Xie Y, Aigle B, Apel AK, Balibar CJ, Balskus EP, Barona-Gómez F, Bechthold A, Bode HB, Borriss R, Brady SF, Brakhage AA, Caffrey P, Cheng YQ, Clardy J, Cox RJ, De Mot R, Donadio S, Donia MS, van der Donk WA, Dorrestein PC, Doyle S, Driessen AJ, Ehling-Schulz M, Entian KD, Fischbach MA, Gerwick L, Gerwick WH, Gross H, Gust B, Hertweck C, Höfte M, Jensen SE, Ju J, Katz L, Kaysser L, Klassen JL, Keller NP, Kormanec J, Kuipers OP, Kuzuyama T, Kyrpides NC, Kwon HJ, Lautru S, Lavigne R, Lee CY, Linquan B, Liu X, Liu W, Luzhetskyy A, Mahmud T, Mast Y, Méndez C, Metsä-Ketelä M, Micklefield J, Mitchell DA, Moore BS, Moreira LM, Müller R, Neilan BA, Nett M, Nielsen J, O'Gara F, Oikawa H, Osbourn A, Osburne MS, Ostash B, Payne SM, Pernodet JL, Petricek M, Piel J, Ploux O, Raaijmakers JM, Salas JA, Schmitt EK, Scott B, Seipke RF, Shen B, Sherman DH, Sivonen K, Smanski MJ, Sosio M, Stegmann E, Süssmuth RD, Tahlan K, Thomas CM, Tang Y, Truman AW, Viaud M, Walton JD, Walsh CT, Weber T, van Wezel GP, Wilkinson B, Willey JM, Wohlleben W, Wright GD, Ziemert N, Zhang C, Zotchev SB, Breitling R, Takano E, Glöckner FO. Minimum Information about a Biosynthetic Gene cluster. Nature Chemical Biology, 2015, 11(9): 625-631.


2014


Zhang G#, Zhang W#, Zhang Q, Shi T, Ma L, Zhu Y, Li S, Zhang H, Zhao YL, Shi R, Zhang C*. Mechanistic Insights Into Polycycle Formation by Reductive Cyclization in Ikarugamycin Biosynthesis. Angewandte Chemie International Edition, 2014, 53(19): 4840-4844. (Recommended by Faculty of 1000; "Hot off the press" in Natural Product Reports, 2014, 31(9): 1083-1087).



Chen Y#, Zhang W#, Zhu Y*, Zhang Q, Tian X, Zhang S, Zhang C*. Elucidating Hydroxylation and Methylation Steps Tailoring Piericidin A1 Biosynthesis. Organic Letters, 2014, 16(3): 736-739.



Fu P#, Zhu Y#, Mei X, Wang Y, Jia H, Zhang C*, Zhu W*. Acyclic Congeners from Actinoalloteichus cyanogriseus Provide Insights into Cyclic Bipyridine Glycoside Formation. Organic Letters, 2014, 16(16): 4264-4267.



Zhang W, Ma L, Li S, Liu Z, Chen Y, Zhang H, Zhang G, Zhang Q, Tian X, Yuan C, Zhang S, Zhang WM, Zhang C*. Indimicins A–E, Bisindole Alkaloids from the Deep Sea-Derived Streptomyces sp. SCSIO 03032. Journal of Natural Products, 2014, 77(8): 1887-1892. (Featured as "Key Drug Discovery Article" by Global Medical Discovery)



Zhang W, Li S, Zhu Y, Chen YC, Chen YL, Zhang HB, Zhang G, Tian X, Pan Y, Zhang S, Zhang WM, Zhang C*. Heronamides D–F, Polyketide Macrolactams from the Deep-Sea Derived Streptomyces sp. SCSIO 03032. Journal of Natural Products, 2014, 77(2): 388–391.





2013


Zhu Y, Zhang Q, Li S, Lin Q, Fu P, Zhang G, Zhang H, Shi R, Zhu W*, Zhang C*. Insights into Caerulomycin A Biosynthesis: A Two-component Monooxygenase CrmH-Catalyzed Oxime Formation. Journal of the American Chemical Society, 2013, 135(50): 18750-18753. ("Hot off the press" in Natural Products Reports, 2014, 31(4): 414-418).



Chen R#, Zhang H#, Zhang G, Li S, Zhang G, Zhu Y, Liu J, Zhang C*. Characterizing Amosamine Biosynthesis in Amicetin Reveals AmiG as a Reversible Retaining Glycosyltransferase. Journal of the American Chemical Society, 2013, 135 (33): 12152–12155.



Li S#, Xiao J#, Zhu Y, Zhang G, Yang C, Zhang H, Ma L, Zhang C*. Dissecting Glycosylation Steps in Lobophorin Biosynthesis Implies an Iterative Glycosyltransferase. Organic Letters, 2013, 15 (6): 1374-1377.



Wu Z, Li S, Li J, Chen Y, Saurav K, Zhang Q, Zhang H, Zhang W, Zhang W, Zhang S, Zhang C*. Antibacterial and Cytotoxic New Napyradiomycins from the Marine-Derived Streptomyces sp. SCSIO 10428. Marine Drugs, 2013, 11(6): 2113-2125.




Xiao J, Zhang Q, Zhu Y, Li S, Zhang G, Zhang H, Saurav K, Zhang C*. Characterization of the Sugar-O-Methyltransferase LobS1 in Lobophorin Biosynthesis. Applied Microbiology and Biotechnology, 2013, 97(20): 9043-9053.




Wu ZC, Li S, Nam SJ, Liu Z, Zhang C*. Nocardiamides A and B, Two Cyclohexapeptides from the Marine-Derived Actinomycete Nocardiopsis sp. CNX037. Journal of Natural Products, 2013, 76 (4): 694–701.



Zhang Q, Li S, Chen Y, Tian X, Zhang H, Zhang G, Zhu Y, Zhang S, Zhang W, Zhang C*. New Diketopiperazine Derivatives from a Deep-Sea-Derived Nocardiopsis alba SCSIO 03039. The Journal of Antibiotics, 2013, 66 (1): 31-36.



Tian XP, Long LJ, Li SM, Zhang J, Xu Y, He J, Li J, Wang FZ, Li WJ, Zhang C, Zhang S*. Pseudonocardia antitumoralis sp. nov., a new deoxynyboquinone-producing actinomycete isolated from a deep-sea sedimental sample in South China Sea. International Journal of Systematic and Evolutionary Microbiology, 2013, 63 (Pt 3):893-899.

2012


Li H#, Zhang Q#, Li S, Zhu Y, Zhang G, Zhang H, Tian X, Zhang S, Ju J, Zhang C*. Identification and Characterization of Xiamycin A and Oxiamycin Gene Cluster Reveals an Oxidative Cyclization Strategy Tailoring Indolosesquiterpene Biosynthesis. Journal of the American Chemical Society, 2012, 134 (21): 8996-9005.



Zhu Y#, Fu P#, Lin Q, Zhang G, Zhang H, Li S, Ju J, Zhu W*, Zhang C*. Identification of Caerulomycin A Gene Cluster Implicates a Tailoring Amidohydrolase. Organic Letters, 2012, 14 (11): 2666-2669. ("Hot off the press" in Natural Product Reports, 2012, 29: 829-833)



Zhang W, Liu Z, Li S, Yang T, Zhang Q, Ma L, Tian X, Zhang H, Huang C, Zhang S, Ju J, Shen Y, Zhang C*. Spiroindimicins A–D: New Bisindole Alkaloids from a Deep-Sea Derived Actinomycete. Organic Letters, 2012, 14(13): 3364-3367. ("Hot off the press" in Natural Product Reports, 2012, 29: 1033-1037)



Zhang Q#, Li H#, Li S, Zhu Y, Zhang G, Zhang H, Zhang W, Shi R, Zhang C*. Carboxyl Formation from Methyl via Triple Hydroxylations by XiaM in Xiamycin A Biosynthesis. Organic Letters, 2012, 14 (24): 6142–6145. ("Hot off the press" in Natural Product Reports, 2013, 30: 485-489)



Zhang G#, Zhang H#, Li S, Xiao J, Zhang G, Zhu Y, Niu S, Ju J, Zhang C*. Characterization of the Amicetin Biosynthetic Gene Cluster from Streptomyces vinaceusdrappus NRRL 2363 Implicates Two Alternative Strategies for Amide Bond Formation. Applied and Environmental Microbiology, 2012, 78(7): 2393-2401.



Zhang Q, Mándi A, Li S, Tian X, Zhang W, Chen Y, Zhang H, Li H, Zhang WM, Zhang S, Ju J, Kurtán T*, Zhang C*. N–N-coupled indolosesquiterpene atropo-diastereomers from a marine-derived actinomycete. European Journal of Organic Chemistry, 2012, 2012(27): 5256-5262.



Zhang W#, Liu Z#, Li S, Lu Y, Chen Y, Zhang H, Zhang G, Zhu Y, Zhang G, Zhang W, Liu J, Zhang C*. Fluostatins I-K from the South China Sea-Derived Micromonospora rosaria SCSIO N160. Journal of Natural Products, 2012, 75(11): 1937–1943.



Tian XP, Xu Y, Zhang J, Li J, Chen Z, Kim CJ, Li WJ, Zhang C, Zhang S*. Streptomyces oceani sp. nov., a new obligate marine actinomycete isolated from a deep-sea sample of seep authigenic carbonate nodule in South China Sea. Antonie Van Leeuwenhoek, 2012 , 102(2): 335-343.

Li J, Zhang GT, Yang J, Tian XP, Wang FZ, Zhang C, Zhang S*, Li WJ*. Marininema mesophilum gen. nov., sp. nov., a thermoactinomycete isolated from deep sea sediment, and emended description of the family Thermoactinomycetaceae. International Journal of Systematic and Evolutionary Microbiology, 2012, 62(Pt 6):1383-1388.

Tian XP, Long LJ, Wang FZ, Xu Y, Li J, Zhang J, Zhang C, Zhang S*, Li WJ*. Streptomyces nanhaiensis sp. nov., a novel marine streptomycete isolated from a deep sea sediment in Southern China Sea. International Journal of Systematic and Evolutionary Microbiology, 2012, 62(Pt 4): 864-868.



2011


Xiao Y#, Li S#, Niu S, Ma L, Zhang G, Zhang H, Zhang GY, Ju J, Zhang C*. Characterization of Tiacumicin B Biosynthetic Gene Cluster Affording Diversified Tiacumicin Analogues and Revealing a Tailoring Dihalogenase.  Journal of the American Chemical Society, 2011, 133 (4): 1092–1105. ("Hot off the press" in Natural Product Reports, 2011, 28: 659-662)



Niu S#, Hu T#, Li S, Xiao Y, Ma L, Zhang G. Zhang H, Zhang S, Yang X, Zhang C*. Characterization of a Sugar-O-methyltransferase TiaS5 Affords New Tiacumicin Analogues with Improved Antibacterial Property and Reveals Substrate Promiscuity. ChemBioChem, 2011, 12(11): 1740-1748.



Li S, Tian XP, Niu S, Zhang W, Chen Y, Zhang H, Zhang W, Li WJ, Zhang S, Ju J, Zhang C*. Pseudonocardians A-C, New Diazaanthraquinone Derivatives from a Deep-Sea Actinomycete Pseudonocardia sp. SCSIO 01299. Marine Drugs, 2011, 2011, 9(8): 1428-1439.



Niu S, Li S, Chen Y, Tian X, Zhang H, Zhang G, Zhang W, Yang X, Zhang S, Ju J, Zhang C*. Lobophorins E and F, new spirotetronate antibiotics from a South China Sea-derived Streptomyces sp. SCSIO 01127. The Journal of Antibiotics, 2011, 64(11):711-716.



Gong L, Xiao Y, Liu Q, Li S, Zhang C*, Liu J*. Preliminary X-ray crystallographic analysis of the glycosyltransferase from a marine Streptomyces species. Acta Crystallogr Sect F Struct Biol Cryst Commun., 2011, 67(1): 136-139.



Postdoctoral and graduate:


Williams GJ, Yang J, Zhang C, Thorson JS*. Recombinant E. coli Prototype Strains for in Vivo Glycorandomization. ACS Chemical Biology, 2011, 6(1): 94-100.



Zhang C, Griffith BR, Fu Q, Albermann C, Fu X, Lee IK, Li L, Thorson JS*. Exploiting the Reversibility of Natural Product Glycosyltransferase-catalyzed Reactions. Science, 2006, 313(5791): 1291-1294. (Featured in Nature, 2006, 443: 285; ACS Chemical Biology , 2006, 1, 499; Angewandte Chemie International Edition , 2007, 46:2147; highlighted in Nature, 2006, 443: 5 and Nature Chemical Biology , 2006, 2: 517; Nature Methods , 2006, 3: 873 and Chemical & Engineering News , 2006, 84:13; and reported in various press including United Press International , Wisconsin State Journal , Wisconsin Technology Network , UW news , The Daily Cardinal , Milwaukee Journal Sentinel , etc.)



Zhang C, Jiang J, Morreti R, Thorson JS*. The In Vitro Characterization of Polyene Glycosyltransferases AmphDI and NysDI. ChemBioChem, 2008, 9(15): 2506-2514.



Zhang C, Bitto E, Goff RD, Griffith BR, Albermann C, Bingman CA, Phillips Jr GN*, Thorson JS*. Biochemical and Structural Insights of the Early Glycosylation Steps in Calicheamicin Biosynthesis. Chemistry and Biology, 2008, 15(8): 842-853.



Zhang C, Fu Q, Albermann C, Li L, Thorson JS*. The In Vitro Characterization of the Erythronolide Mycarosyltransferase EryBV and Its Utility in Macrolide Diversification. ChemBioChem, 2007, 8(4): 385-390.



Zhang C, Albermann C, Fu X, Thorson JS*. The In Vitro Characterization of the Iterative Avermectin Glycosyltransferase AveBI Reveals Reaction Reversibility and Sugar Nucleotide Flexibility.  Journal of the American Chemical Society, 2006, 128(51): 16420-16421.



Zhang C, Albermann C, Fu X, Peters NR, Chisholm JD, Zhang G, Gilbert EJ, Wang PG, Van Vranken DL, Thorson JS*. RebG- and RebM-catalyzed Indolocarbazole Diversification. ChemBioChem, 2006, 7(5): 795-804.



Zhang C, Weller RL, Thorson JS, Rajski SR*. Natural Product Diversification Using Non-natural Cofactor Analogues of S-adenosyl-L-methionine.  Journal of the American Chemical Society, 2006, 128(9): 2760-2761.



Zhang C, Podeschwa M, Block O, Altenbach HJ, Piepersberg W, Wehmeier UF*. Identification of a 1-epi-valienol 7-kinase activity in the producer of acarbose, Actinoplanes sp. SE50/110. FEBS Letters, 2003, 540(1-3): 53-57.

Zhang C, Podeschwa M, Altenbach HJ, Piepersberg W and Wehmeier UF*. The acarbose-biosynthetic enzyme AcbO from Actinoplanes sp. SE 50/110 is a 2-epi-5-epi-valiolone-7-phosphate 2-epimerase. FEBS Letters, 2003, 540(1-3): 47-52.

Zhang C, Stratmann A, Block O, Brückner R, Podeschwa M, Altenbach HJ, Wehmeier UF and Piepersberg W*. Biosynthesis of the C7-cyclitol Moiety of Acarbose in Actinoplanes Species SE50/110. 7-O-phosphorylation of the initial cyclitol precursor leads to proposal of a new biosynthetic pathway. Journal of Biological Chemistry, 2002, 277(25): 22853-22862.



Williams GJ, Zhang C, Thorson JS*. Expanding the Promiscuity of A Natural Product Glycosyltransferase by Directed Evolution. Nature Chemical Biology, 2007, 3(10): 657-662. (Featured in Nature Chemical Biology , 2007, 3: 604; and Chemical & Engineering News , 2007, 85: 9; and reported in various press including: United Press International , Chemistry World, UW news , and Wisconsin State Journal , etc.)



Borisova SA, Zhang C, Takahashi H, Zhang H, Wong AW, Thorson JS, Liu H-w*. Substrate Specificity of the Macrolide-Glycosylating Enzyme Pair DesVII/DesVIII: Opportunities, Limitations and Mechanistic Hypotheses. Angewandte Chemie International Edition, 2006, 45(17): 2748-2753.



Gao Q, Zhang C, Blanchard S, Thorson JS*. Deciphering indolocarbazole and enediyne aminopentose biosynthesis through comparative genomics: New insights and tools from the Actinomadura melliaura AT2433 biosynthetic locus. Chemistry and Biology, 2006, 13(7):733-743.



Williams GJ, Goff RD, Zhang C, Thorson JS*. Optimizing glycosyltransferase specificity via ‘hot spot’ saturation mutagenesis presents a new catalyst for novobiocin glycorandomization. Chemistry and Biology, 2008, 15(4): 393-401.



Singh S, McCoy JG, Zhang C, Bingman CA, Phillips Jr GN*, Thorson JS*. Structure and Mechanism of the Rebeccamycin Sugar 4-O-Methyltransferase RebM. Journal of Biological Chemistry, 2008, 283(33): 22628-22636.



Singh S, Hager MH, Zhang C, Griffith BR, Lee MS, Hallenga K, Markley JL and Thorson JS*. Structural insight into self-sacrifice mechanism of enediyne resistance. ACS Chemical Biology, 2006, 1(7): 451-460.



Fu X, Albermann C, Zhang C, Thorson JS*. Diversifying Vancomycin via Chemoenzymatic Strategies. Organic Letters, 2005, 7(8): 1513-1515.



Fu X, Albermann C, Jiang J, Liao J, Zhang C, Thorson JS*. Antibiotic optimization via in vitro glycorandomization. Nature Biotechnology, 2003, 21(12): 1467-1469.

指导学生

已指导学生

林钦恒  硕士研究生  070703-海洋生物学  

肖吉  硕士研究生  070703-海洋生物学  

吴正超  博士研究生  070703-海洋生物学  

马亮  硕士研究生  070703-海洋生物学  

张庆波  博士研究生  070703-海洋生物学  

现指导学生

陈耀龙  硕士研究生  070703-海洋生物学  

于洁  博士研究生  070703-海洋生物学  

陈瑞东  博士研究生  070703-海洋生物学  

聂方玲  硕士研究生  070703-海洋生物学  

杨春芳  博士研究生  070703-海洋生物学  

郑六眷  硕士研究生  070702-海洋化学  

马亮  博士研究生  070703-海洋生物学  

主持项目