实验室致力于“病毒感染与防治研究的新策略和新系统“的开发和应用基础研究。主要研究方向包括：1）基于合成生物学原理和技术手段，开发病毒生命过程调控元件，进行病毒定向工程，促进工程病毒的医学应用，包括疫苗、病毒载体等；2）基于干细胞、原代细胞、微流控芯片，合成构建人器官组织体外仿生系统—人器官芯片，为疾病研究及防治策略开发提供人器官生理相关的临床前研究平台，为个性化疫疗提供新工具。研究成果以通讯作者、第一作者（含共同一作）发表于Science、Nature Biotechnology、Nature Biomedical Engieering、Nature Comunications、Science Advances、Biomaterials等期刊；申请及授权专利20余项。

招收博士生和硕士生

071005-微生物学
071010-生物化学与分子生物学
100705-微生物与生化药学

病毒疫苗
人器官芯片开发及应用
生物技术药物

2012-09--2017-06   北京大学 药学院/天然药物及仿生药物国家重点实验室   博士
2008-09--2012-06   四川大学 华西药学院   本科

   

   

（1） Wyss Technology Development Fellowship, 其他, 2019
（2） 中国科学十大进展, 国家级, 2017
（3） 中国大学生年度人物, 国家级, 2017
（4） International Society for Vaccines Papers of The Year, 其他, 2016

   

[1] Jing Li, Haiqing Bai, Zihao Wang, Beibei Xu, Kristen N Peters Olson, Chengyao Liu, Yinlei Su, Jiawei Hao, Jinying Shen, Xuetong Xi, Jie Zhen, Rong Yu, Rong Yu, Xin Xie, Wenxia Tian, Fei Yu, Xiaoheng Liu, Lihe Zhang, Demin Zhou, Longlong Si. Advancements in organs-on-chips technology for viral disease and anti-viral research. ORGANS-ON-A-CHIP. 2023, 5: http://dx.doi.org/10.1016/j.ooc.2023.100030.
[2] Bai, Haiqing, Si, Longlong, Jiang, Amanda, Belgur, Chaitra, Zhai, Yunhao, Plebani, Roberto, Oh, Crystal Yuri, Rodas, Melissa, Patil, Aditya, Nurani, Atiq, Gilpin, Sarah E, Powers, Rani K, Goyal, Girija, PrantilBaun, Rachelle, Ingber, Donald E. Mechanical control of innate immune responses against viral infection revealed in a human lung alveolus chip. NATURE COMMUNICATIONS[J]. 2022, 13(1): http://dx.doi.org/10.1038/s41467-022-29562-4.
[3] Li, Zhen, Bai, Haiqing, Xi, Xuetong, Tian, Wenxia, Zhang, John Z H, Zhou, Demin, Si, Longlong. PROTAC vaccine: A new way to live attenuated vaccines. CLINICAL AND TRANSLATIONAL MEDICINE[J]. 2022, 12(10): http://dx.doi.org/10.1002/ctm2.1081.
[4] Roberto Plebani, Haiqing Bai, Longlong Si, Jing Li, Chunhe Zhang, Mario Romano, Evgenii Gusev. 3D Lung Tissue Models for Studies on SARS-CoV-2 Pathophysiology and Therapeutics. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES[J]. 2022, 23(17): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456220/.
[5] Si, Longlong, Shen, Quan, Li, Jing, Chen, Li, Shen, Jinying, Xiao, Xue, Bai, Haiqing, Feng, Tang, Ye, Adam Yongxin, Li, Le, Zhang, Chunhe, Li, Zhen, Wang, Ping, Oh, Crystal Yuri, Nurani, Atiq, Niu, Siwen, Zhang, Chengxin, Wei, Xiaoqiong, Yuan, Wanqiong, Liao, Hao, Huang, Xiaojie, Wang, Ning, Tian, Wen-xia, Tian, Hongwei, Li, Li, Liu, Xiaoheng, Plebani, Roberto. Generation of a live attenuated influenza A vaccine by proteolysis targeting. Nature Biotechnology[J]. 2022, https://www.nature.com/articles/s41587-022-01381-4.
[6] Si, Longlong, Bai, Haiqing, Oh, Crystal Yuri, Jiang, Amanda, Hong, Fan, Zhang, Tian, Ye, Yongxin, Jordan, Tristan X, Logue, James, McGrath, Marisa, Belgur, Chaitra, Calderon, Karina, Nurani, Atiq, Cao, Wuji, Carlson, Kenneth E, PrantilBaun, Rachelle, Gygi, Steven P, Yang, Dong, Jonsson, Colleen B, tenOever, Benjamin R, Frieman, Matthew, Ingber, Donald E. Self-assembling short immunostimulatory duplex RNAs with broad-spectrum antiviral activity. MOLECULAR THERAPY-NUCLEIC ACIDS[J]. 2022, 29: 923-940, http://dx.doi.org/10.1016/j.omtn.2022.08.031.
[7] Yang, Xiao, Ding, Chunmei, Wu, Mingzhen, Xu, Xinyuan, Ke, Xiang, Xu, Huilin, Li, Juan, Lou, Feng, Zhou, Kai, Jiang, Haolun, Peng, Xu, Wang, Xianlong, Si, Longlong, Li, Jianshu. Biomineral interface with superior cell adhesive and antibacterial properties based on enzyme-triggered digestion of saliva acquired pellicle-inspired polypeptide coatings. CHEMICAL ENGINEERING JOURNAL[J]. 2021, 415: http://dx.doi.org/10.1016/j.cej.2021.128955.
[8] Si, Longlong, Bai, Haiqing, Rodas, Melissa, Cao, Wuji, Oh, Crystal Yuri, Jiang, Amanda, Moller, Rasmus, Hoagland, Daisy, Oishi, Kohei, Horiuchi, Shu, Uhl, Skyler, BlancoMelo, Daniel, Albrecht, Randy A, Liu, WenChun, Jordan, Tristan, NilssonPayant, Benjamin E, Golynker, Ilona, Frere, Justin, Logue, James, Haupt, Robert, McGrath, Marisa, Weston, Stuart, Zhang, Tian, Plebani, Roberto, Soong, Mercy, Nurani, Atiq, Kim, Seong Min, Zhu, Danni Y, Benam, Kambez H, Goyal, Girija, Gilpin, Sarah E, PrantilBaun, Rachelle, Gygi, Steven P, Powers, Rani K, Carlson, Kenneth E, Frieman, Matthew, TenOever, Benjamin R, Ingber, Donald E. A human-airway-on-a-chip for the rapid identification of candidate antiviral therapeutics and prophylactics. NATURE BIOMEDICAL ENGINEERING[J]. 2021, 5(8): 815-829, http://dx.doi.org/10.1038/s41551-021-00718-9.
[9] Si, Longlong, Bai, Haiqing, Oh, Crystal Yuri, Jin, Lei, PrantilBaun, Rachelle, Ingber, Donald E. Clinically Relevant Influenza Virus Evolution Reconstituted in a Human Lung Airway-on-a-Chip. MICROBIOLOGY SPECTRUM[J]. 2021, 9(2): https://doaj.org/article/265603015c6f47feb745c6711a606107.
[10] Wu, Zhong, Zhou, Jin, Zhang, Xiaoyang, Zhang, Zhouwei, Xie, Yingtian, Liu, Jie bin, Ho, Zandra, V, Panda, Arpit, Qiu, Xintao, Cejas, Paloma, Canadas, Israel, Akarca, Fahire Goknur, McFarland, James M, Nagaraja, Ankur K, Goss, Louisa B, Kesten, Nikolas, Si, Longlong, Lim, Klothilda, Liu, Yanli, Zhang, Yanxi, Baek, Ji Yeon, Liu, Yang, Patil, Deepa T, Katz, Jonathan P, Hai, Josephine, Bao, Chunyang, Stachler, Matthew, Qi, Jun, Ishizuka, Jeffrey J, Nakagawa, Hiroshi, Rustgi, Anil K, Wong, KwokKin, Meyerson, Matthew, Barbie, David A, Brown, Myles, Long, Henry, Bass, Adam J. Reprogramming of the esophageal squamous carcinoma epigenome by SOX2 promotes ADAR1 dependence. NATURE GENETICS[J]. 2021, 53(6): 881-+, http://dx.doi.org/10.1038/s41588-021-00859-2.
[11] Tang, Huaqi, Abouleila, Yasmine, Si, Longlong, OrtegaPrieto, Ana Maria, Mummery, Christine L, Ingber, Donald E, Mashaghi, Alireza. Human Organs-on-Chips for Virology. TRENDS IN MICROBIOLOGYnull. 2020, 28(11): 934-946, http://dx.doi.org/10.1016/j.tim.2020.06.005.
[12] Si, Longlong, Meng, Kun, Tian, Zhenyu, Sun, Jiaqi, Li, Huiqiang, Zhang, Ziwei, Soloveva, Veronica, Li, Haiwei, Fu, Ge, Xia, Qing, Xiao, Sulong, Zhang, Lihe, Zhou, Demin. Triterpenoids manipulate a broad range of virus-host fusion via wrapping the HR2 domain prevalent in viral envelopes. SCIENCE ADVANCES[J]. 2018, 4(11): https://www.webofscience.com/wos/woscc/full-record/WOS:000452212000070.
[13] Xiao, Sulong, Tian, Zhenyu, Wang, Yufei, Si, Longlong, Zhang, Lihe, Zhou, Demin. Recent progress in the antiviral activity and mechanism study of pentacyclic triterpenoids and their derivatives. MEDICINAL RESEARCH REVIEWSnull. 2018, 38(3): 951-976, http://dx.doi.org/10.1002/med.21484.
[14] Wu, Ling, Chen, Jingxian, Wu, Yiming, Zhang, Bo, Cai, Xiaochun, Zhang, Ziwei, Wang, Yan, Si, LongLong, Xu, Huan, Zheng, YongXiang, Zhang, ChuanLing, Liang, Chenggang, Li, Jing, Zhang, Lihe, Zhang, Qiang, Zhou, Demin. Precise and combinatorial PEGylation generates a low-immunogenic and stable form of human growth hormone. JOURNAL OF CONTROLLED RELEASE[J]. 2017, 249: 84-93, http://dx.doi.org/10.1016/j.jconrel.2017.01.029.
[15] Wu, Shaoping, Yang, Lili, Sun, Wenji, Si, Longlong, Xiao, Sulong, Wang, Qi, Dechoux, Luc, Thorimbert, Serge, Sollogoub, Matthieu, Zhou, Demin, Zhang, Yongmin. Design, synthesis and biological evaluation of gentiopicroside derivatives as potential antiviral inhibitors. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY[J]. 2017, 130: 308-319, http://dx.doi.org/10.1016/j.ejmech.2017.02.028.
[16] Tian, Zhenyu, Si, Longlong, Meng, Kun, Zhou, Xiaoshu, Zhang, Yongmin, Zhou, Demin, Xiao, Sulong. Inhibition of influenza virus infection by multivalent pentacyclic triterpene-functionalized per-O-methylated cyclodextrin conjugates. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY[J]. 2017, 134: 133-139, http://dx.doi.org/10.1016/j.ejmech.2017.03.087.
[17] Sulong Xiao, Qi Wang, Longlong Si, Xiaoshu Zhou, Yongmin Zhang, Lihe Zhang, Demin Zhou. Synthesis and biological evaluation of novel pentacyclic triterpene α-cyclodextrin conjugates as HCV entry inhibitors. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY. 2016, 124: 1-9, http://dx.doi.org/10.1016/j.ejmech.2016.08.020.
[18] Xiao, Sulong, Si, Longlong, Tian, Zhenyu, Jiao, Pingxuan, Fan, Zibo, Meng, Kun, Zhou, Xiaoshu, Wang, Han, Xu, Renyang, Han, Xu, Fu, Ge, Zhang, Yongmin, Zhang, Lihe, Zhou, Demin. Pentacyclic triterpenes grafted on CD cores to interfere with influenza virus entry: A dramatic multivalent effect. BIOMATERIALS[J]. 2016, 78: 74-85, http://dx.doi.org/10.1016/j.biomaterials.2015.11.034.
[19] Niu, Siwen, Si, Longlong, Liu, Dong, Zhou, Andrew, Zhang, Ziwei, Shao, Zongze, Wang, Shaomeng, Zhang, Lihe, Zhou, Demin, Lin, Wenhan. Spiromastilactones: A new class of influenza virus inhibitors from deep-sea fungus. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY[J]. 2016, 108: 229-244, http://dx.doi.org/10.1016/j.ejmech.2015.09.037.
[20] Wu, Yiming, Zhu, Hua, Zhang, Bo, Liu, Fei, Chen, Jingxian, Wang, Yufei, Wang, Yan, Zhang, Ziwei, Wu, Ling, Si, Longlong, Xu, Huan, Yao, Tianzhuo, Xiao, Sulong, Xia, Qing, Zhang, Lihe, Yang, Zhi, Zhou, Demin. Synthesis of Site-Specific Radiolabeled Antibodies for Radioimmunotherapy via Genetic Code Expansion. BIOCONJUGATE CHEMISTRY[J]. 2016, 27(10): 2460-2468, https://www.webofscience.com/wos/woscc/full-record/WOS:000385992000028.
[21] Si, Longlong, Xu, Huan, Zhou, Xueying, Zhang, Ziwei, Tian, Zhenyu, Wang, Yan, Wu, Yiming, Zhang, Bo, Niu, Zhenlan, Zhang, Chuanling, Fu, Ge, Xiao, Sulong, Xia, Qing, Zhang, Lihe, Zhou, Demin. Generation of influenza A viruses as live but replication-incompetent virus vaccines. SCIENCE[J]. 2016, 354(6316): 1170-1173, https://www.webofscience.com/wos/woscc/full-record/WOS:000388916400047.
[22] Ji, Shuai, Li, Ru, Wang, Qi, Miao, Wenjuan, Li, Ziwei, Si, Longlong, Qiao, Xue, Yu, Siwang, Zhou, Demin, Ye, Min. Anti-H1N1 virus, cytotoxic and Nrf2 activation activities of chemical constituents from Scutellaria baicalensis. JOURNAL OF ETHNOPHARMACOLOGY[J]. 2015, 176: 475-484, https://www.webofscience.com/wos/woscc/full-record/WOS:000367106600044.
[23] Zheng, Yongxiang, Yu, Fei, Wu, Yiming, Si, Longlong, Xu, Huan, Zhang, Chuanling, Xia, Qing, Xiao, Sulong, Wang, Qi, He, Qiuchen, Chen, Peng, Wang, Jiangyun, Taira, Kazunari, Zhang, Lihe, Zhou, Demin. Broadening the versatility of lentiviral vectors as a tool in nucleic acid research via genetic code expansion. NUCLEIC ACIDS RESEARCH[J]. 2015, 43(11): E73-U42, https://www.webofscience.com/wos/woscc/full-record/WOS:000357886900004.
[24] Chen, Xueqing, Si, Longlong, Liu, Dong, Proksch, Peter, Zhang, Lihe, Zhou, Demin, Lin, Wenhan. Neoechinulin B and its analogues as potential entry inhibitors of influenza viruses, targeting viral hemagglutinin. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY[J]. 2015, 93: 182-195, http://dx.doi.org/10.1016/j.ejmech.2015.02.006.
[25] Zhang, Bo, Xu, Huan, Chen, Jingxian, Zheng, Yongxiang, Wu, Yiming, Si, Longlong, Wu, Ling, Zhang, Chuanling, Xia, Gang, Zhang, Lihe, Zhou, Demin. Development of next generation of therapeutic IFN-alpha 2b via genetic code expansion. ACTA BIOMATERIALIA[J]. 2015, 19: 100-111, https://www.webofscience.com/wos/woscc/full-record/WOS:000354152100011.
[26] Song, Wei, Si, Longlong, Ji, Shuai, Wang, Han, Fang, Xiaomei, Yu, Liyan, Li, Renyong, Liang, Lina, Zhou, Demin, Ye, Min. Uralsaponins M-Y, Antiviral Triterpenoid Saponins from the Roots of Glycyrrhiza uralensis. JOURNAL OF NATURAL PRODUCTS[J]. 2014, 77(7): 1632-1643, http://dx.doi.org/10.1021/np500253m.
[27] Yu, Maorong, Si, Longlong, Wang, Yufei, Wu, Yiming, Yu, Fei, Jiao, Pingxuan, Shi, Yongying, Wang, Han, Xiao, Sulong, Fu, Ge, Tian, Ke, Wang, Yitao, Guo, Zhihong, Ye, Xinshan, Zhang, Lihe, Zhou, Demin. Discovery of Pentacyclic Triterpenoids as Potential Entry Inhibitors of Influenza Viruses. JOURNAL OF MEDICINAL CHEMISTRY[J]. 2014, 57(23): 10058-10071, http://dx.doi.org/10.1021/jm5014067.
[28] Yu, Fei, Peng, Yiyun, Wang, Qi, Shi, Yongying, Si, Longlong, Wang, Han, Zheng, Yongxiang, Lee, Emily, Xiao, Sulong, Yu, Maorong, Li, Yingbo, Zhang, Chuanling, Tang, Hengli, Wang, Chunguang, Zhang, Lihe, Zhou, Demin. Development of bivalent oleanane-type triterpenes as potent HCV entry inhibitors. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY[J]. 2014, 77: 258-268, http://dx.doi.org/10.1016/j.ejmech.2014.03.017.
[29] Yang, Aigang, Si, Longlong, Shi, Zhenping, Tian, Li, Liu, Dong, Zhou, Demin, Proksch, Peter, Lin, Wenhan. Nitrosporeusines A and B, Unprecedented Thioester-Bearing Alkaloids from the Arctic Streptomyces nitrosporeus. ORGANIC LETTERS[J]. 2013, 15(20): 5366-5369, https://www.webofscience.com/wos/woscc/full-record/WOS:000326121700056.
[30] Xiaojing Lin, Murong Zhu, Xiujuan Zhao, Longlong Si, Meiyue Dong, Varada Anirudhan, Qinghua Cui, Lijun Rong, Ruikun Du. Optimization and applications of an in vivo bioluminescence imaging model of influenza A virus infections. VIROLOGICA SINICA. http://dx.doi.org/10.1016/j.virs.2023.04.007.