Photobiology of marine microorganisms

Deep oceans are dark, barren and cold ecosystems with extreme high hydrostastic pressure. Our main interest is using genomics, microbiology, biochemistry, photobiology, physics and microscopy approaches to study effect of electromagnetic waves on the life of microorganisms.

·         Habilitation, April 1992, University of Lyon I, France

·         Ph.D. March 1988, National Institute of Applied Science at Lyon, France

·         MSc. May 1984, National Institute of Applied Science at Lyon, France

·         BSc. July 1982, Shandong University (PRC).

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• May 1988-Decemmber 1990 : Post-Doctoral Research Fellow, Prof. M.Saier’s laboratory, Department of Biology, University of California, San Diego, USA.
• October 1990-August 1998 : Researcher (Chargé de Recherche 1ème classe, CNRS), Laboratoire de Génétique Moléculaire des Microorganismes et des Interactions Cellulaires, URA CNRS 1486, I.N.S.A. de Lyon, France 1990-1995). Laboratoire de Chimie Bactérienne, UPR9043, CNRS, IBSM, 31, chemin Joseph Aiguier, 13402 Marseille cedex 20, France (1995-present)
• September 1998- : Research Director (Directeur de Recherche, CNRS) Laboratoire de Chimie Bactérienne, UMR7283, IMM, CNRS-AMU 31, chemin Joseph Aiguier, 13402 Marseille cedex 20, France.
• July 2013- : Researcher, Institute of Deep Sea Science and Engineering, CAS, Sanya, China

• October 1990-August 1998 : Researcher (Chargé de Recherche 1ème classe, CNRS), Laboratoire de Génétique Moléculaire des Microorganismes et des Interactions Cellulaires, URA CNRS 1486, I.N.S.A. de Lyon, France 1990-1995). Laboratoire de Chimie Bactérienne, UPR9043, CNRS, IBSM, 31, chemin Joseph Aiguier, 13402 Marseille cedex 20, France (1995-present)
• September 1998- : Research Director (Directeur de Recherche, CNRS) Laboratoire de Chimie Bactérienne, UMR7283, IMM, CNRS-AMU 31, chemin Joseph Aiguier, 13402 Marseille cedex 20, France.
• July 2013- : Researcher, Institute of Deep Sea Science and Engineering, CAS, Sanya, China

Graduate school lectures at:

Aix-Marseille University

Ocean University China

Institute of Oceanology, CAS, China

Institute of Deea sea Science and Engineering, CAS, China

Wu L.-F. and Mandrand-Berthelot M.A. (1986). Genetic and physiological characterization of new Escherichia coli mutants impaired in hydrogenase activity. Biochimie 68 : 167-179. Cited by 133

Wu L.-F., Reizer A., Reizer J., Cai B., Tomich J.M. and Saier M.H.Jr. (1991). Nucleotide sequence of the fruK gene of Rhodobacter capsulatus encoding fructose-1-phosphate kinase : evidence for a kinase superfamily including both phospho-fructose kinases of E. coli. J. Bacteriol. 173 : 3117-3127. Cited by 85

Navarro C., Wu L.-F. and Mandrand-Berthelot M.A. (1993). The nik operon of Escherichia coli encodes a periplasmic binding protein-dependent transport system for nickel. Mol. Microbiol. 9, 1181-1191. Cited by 277

De Pina K., Desjardin V., Mandrand-Berthelot M.A., Giordano G. and Wu L.-F.* (1999) Isolation and characterization of the nikR gene encoding a nickel-responsive regulator in Escherichia coli. J. Bacteriol. 181: 670-674. Cited by 196

Santini C.-L., Ize B., Chanal A., Muller M., Giordano G. and Wu L.-F.* (1998) A novel Sec-independent periplasmic protein translocation pathway in Escherichia coli. EMBO J. 17 : 101-112. Cited by 376

Rodrigue, A., Chanal, A., Beck, K., Muller, M. and Wu, L.-F.* (1999) Co-translocation of a periplasmic enzyme complex by a hitchhiker mechanism through the bacterial Tat-pathway. J. Biol. Chem. 274: 13223-13228. Cited by 292

Santini C.L., Bernadac A., Zhang M., Chanal A., Ize B., Blanco C. and Wu L.-F.* (2001) Translocation of jellyfish green fluorescent protein via the Tat system of Escherichia coli and change of its periplasmic localization in response to osmotic up-shock. J. Biol. Chem. 276: 8159-8164. Cited by 213

Pradel N., Santini C.-L., Bernadac A., Fukumori Y. and Wu L.-F.* (2006) Biogenesis of actin-like bacterial cytoskeletal filaments destined for positioning prokaryotic magnetic organelles. Proc. Natl. Acad. Sci. USA 103:17485-17489. Cited by 111

Lefèvre C.T, Bernadac A., Yu-Zhang K., Pradel N. and Wu L.-F.* (2009) Isolation and characterization of a magnetotactic bacterial culture from the Mediterranean sea. Environ. Microbiol. 11(7), 1646–1657. Cited by 107

Ji B., Zhang S.-D., Arnoux P., Rouy Z., Alberto F., Philippe N., Murat D., Zhang W.-J., Rioux J.-B., Ginet N., Sabaty M., Mangenot S., Pradel N., Tian J., Yang J., Zhang L., Zhang W.-Y., Pan H., Henrissat B., Coutinho P.M., Li Y., Xiao T., Médigue C., Barbe V., Pignol D., Talla1 E.,* and Wu, L.-F.* (2013). Comparative genomic analysis provides insights into the evolution and niche adaptation of marine Magnetospira sp. QH-2 strain. Environment. Microbiol. 16(2):525-44.

Ruan J., Kato T., Santini C.-L., Miyata T., Kawamoto A., Zhang W.-J., Bernadac A, Wu L.-F. and Namba K. (2012). Architecture of a flagellar apparatus in the fast-swimming magnetotactic bacterium MO-1. Proc. Natl. Acad. Sci. USA. 109(50): 20643-8.

Zhang S.-D., C.-L. Santini, W.-J. Zhang, V. Barbe, S. Mangenot, C. Guyomar, M. Garel, H. Chen, X. Li, Q.-J. Yin, Y. Zhao, J. Armengaud, J.-C. Gaillard, S. Martini, N. Pradel, C. Vidaud, F. Alberto, C. Médigue, C Tamburini and L.-F. Wu*. (2016) Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200. Extremophiles. 20 (3): 301-310.

Ji B., Zhang S.-D., Zhang W.-J., Rouy Z., Alberto F., Santini C.-L., Mangenot S., Gagnot S., Philippe N., Pradel N., Zhang L., Tempel S., Li Y., Médigue C., Henrissat B., Coutinho P.M., Barbe V., E Talla^*, and L.-F. Wu^* (2017). The chimeric nature of the genomes of marine magnetotactic coccoid-ovoid bacteria defines a novel group of Proteobacteria. Environ. Microbiol. 19(3): 1103-1119.

Yin Q.-J., Zhang W.-J., Qi X.-Q., Zhang S.-D., Jiang T, Li X.-G., Chen Y., Santini C.-L., Zhou H., Chou I.M. and Wu L.-F. (2018) High hydrostatic pressure inducible trimethylamine N-oxide reductase improves the pressure tolerance of piezosensitive bacteria Vibrio fluvialis. Front Microbiol. 8:2646. doi: 10.3389/fmicb.2017.02646

Chen H., Zhang S.-D., Chen L., Cai Y., Zhang W.-.J, Song T.* and Wu L.-F.* (2018) Efficient genome editing of magnetospirillum magneticum AMB-1 by CRISPR-Cas9 system for analyzing magnetotactic behavior. Front Microbiol. 9:1569. doi: 10.3389/fmicb.2018.01569.

Li X.G., Zhang W. J., Qi X.Q. and Wu L.-F. (2019) Genome analysis of Crassaminicella sp. SY095, an anaerobic mesophilic marine bacterium isolated from a deep-sea hydrothermal vent on the Southwest Indian Ridge. Marine Genomics, https://doi.org/10.1016/j.margen.2019.100733

Zhang W. J, Cui X. H., Zhang C., Mangenot S., Jiang Y., Kato T., Wu L.-F. (2019) Complete genome sequence of Shewanella benthica DB21MT-2, an obligate piezophilic bacterium isolated from the deepest Mariana Trench sediment. Marine Genomics 44: 52-56, doi.org/10.1016/j.margen.2018.09.001.

Qian X.-X., Santini C.-L., Kosta A., Menguy N., Le Guenno H., Zhang W., Li J., Chen Y.R., Liu J., Alberto F., Espinosa L., Xiao T. and Wu L.-F.* (2020) Juxtaposed membranes underpin cellular adhesion and display unilateral cell division of multicellular magnetotactic prokaryotes. Environ. Microbiol. 22(4): 1481-1494. doi: 10.1111/1462-2920.14710.

Zhang, W.J. and Wu L.-F.* (2020) Flagella and swimming behavior of marine magnetotactic bacteria. Biomolecules. 10(3): 460. https://doi.org/10.3390/biom10030460.

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1.         Dai J., Tang H.-Z., Li X.G., Santini C.-L., Cui W.-P., Liu N., Qi X.Q., Cui X.-H., Grossi A., Notareschi P. Zhang W. J.*, and Wu L.-F.* (2020) Using resazurin as a reducing capacity indicator for analyzing physiological status of deep-sea bacterium Photobacterium phosphoreum ANT-2200. J. Oceanol. Limnol. (in revision).

2.         Zhang, W.J. and Wu L.-F.* (2020) Flagella and swimming behavior of marine magnetotactic bacteria. Biomolecules. 10(3): 460. https://doi.org/10.3390/biom10030460.

3.         Qian X.-X., Santini C.-L., Kosta A., Menguy N., Le Guenno H., Zhang W., Li J., Chen Y.R., Liu J., Alberto F., Espinosa L., Xiao T. and Wu L.-F.* (2020) Juxtaposed membranes underpin cellular adhesion and display unilateral cell division of multicellular magnetotactic prokaryotes. Environ. Microbiol. 22(4): 1481-1494. doi: 10.1111/1462-2920.14710.

4.         Li X.G., Zhang W. J., Qi X.Q. and Wu L.-F. (2019) Genome analysis of Crassaminicella sp. SY095, an anaerobic mesophilic marine bacterium isolated from a deep-sea hydrothermal vent on the Southwest Indian Ridge. Marine Genomics, https://doi.org/10.1016/j.margen.2019.100733

5.         Pan H., Dong Y., Teng Z., Li J., Zhang W., Xiao T. and L.-F. Wu (2019) A species of magnetotactic deltaproteobacterium was detected at the highest abundance during an algal bloom. FEMS Microb. Lett. 366: (22) fnz253, https://doi.org/10.1093/femsle/fnz253

6.         Qian X.-X., Liu J., Menguy N., Li J., Alberto F., Teng Z., Xiao T., Zhang W.* and Wu L.-F.* (2019) Identification of novel species of marine magnetotactic bacteria affiliated with Nitrospirae phylum. Environ. Microbiol. Rep. 11(3):330-337. doi: 10.1111/1758-2229.12755.

7.         Fuduche M., S. Davidson, C. Boileau, L.-F. Wu and Y. Combet-Blanc (2019) A novel highly efficient device for growing micro-aerophilic microorganisms. Front. Microbiol. 10:534. doi: 10.3389/fmicb.2019.00534.

8.         Chen C., L. Chen, P. Wang, L.-F. Wu and T. Song (2019) Steering of magnetotactic bacterial microrobots by focusing magnetic field for targeted pathogen killing. J. Magnet. Magnet. Mater. 479: 74-83. https://doi.org/10.1016/j.jmmm.2019.02.004.

9.         Yin Q. J., Zhang W. J*., Li X. G., Zhou L. H., Qi X. Q., Zhang C. and Wu L.-F. (2019) Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria. J. Oceanol. Limnol. 37 (1): 210–222. doi: 10.1007/s00343-019-7377-9.

10.       Zhang W. J*, Cui X. H., Zhang C., Mangenot S., Jiang Y., Kato T., Wu L.-F. (2019) Complete genome sequence of Shewanella benthica DB21MT-2, an obligate piezophilic bacterium isolated from the deepest Mariana Trench sediment. Marine Genomics 44: 52-56, doi.org/10.1016/j.margen.2018.09.001.

11.       Du H, Zhang W, Zhang W, Zhang W, Pan H, Pan Y, Bazylinski DA, Wu L-F, Xiao T, Lin W. (2019) Magnetosome Gene Duplication as an Important Driver in the Evolution of Magnetotaxis in the Alphaproteobacteria. mSystems, 4:e00315-19.

12.       Zhang W, Liu J, Dong Y, Li X, Xu C, Xiao T, Pan H, Wu L-F. Archaeal community structure in sediments from a seamount in the Mariana Volcanic Arc. J. Oceanol. Limnol. 2019, 37:1197-1210.

13.       Zhang W-Y, Pan H-M, Dong Y, Du H-J, Chen Y-R, Wu L.-F., Xiao T. (2019) Diversity of magnetotactic bacteria in intertidal sediments of Lake Yuehu, Rongcheng. Marine Science, 43:1-10. (In Chinese)

14.       Li X. G., Zhang W. J.*, Xiao X., Jian H., Jiang T., Tang H.Z., Qi X.Q. and Wu, L.-F. (2018) Pressure-regulated gene expression and enzymatic activity of the two periplasmic nitrate reductases in the deep-sea bacterium Shewanella piezotolerans WP3. Front. Microbiol. DOI: 10.3389/fmicb.2018.03173.

15.       Teng Z., Zhang Y., Zhang W., Pan H., Xu J., Huang H., Xiao T., and Wu L.-F. (2018) Diversity and Characterization of Multicellular Magnetotactic Prokaryotes from Coral Reef Habitats of the Paracel Islands, South China Sea. Front. Microbiol. 9:2135. doi: 10.3389/fmicb.2018.02135.

16.       Chen H., Zhang S.-D., Chen L., Cai Y., Zhang W.-.J, Song T.* and Wu L.-F.* (2018) Efficient genome editing of magnetospirillum magneticum AMB-1 by CRISPR-Cas9 system for analyzing magnetotactic behavior. Front Microbiol. 9:1569. doi: 10.3389/fmicb.2018.01569.

17.       Liu J., Zhang W., Du H., Leng X., Li J.H., Pan H., Xu J., Wu L.-F. and Xiao T. (2018) Seasonal changes in the vertical distribution of two types of multicellular magnetotactic prokaryotes in the sediment of Lake Yuehu, China. Environ Microbiol Rep. doi: 10.1111/1758-2229.12652.

18.       Yin Q.-J., Zhang W.-J., Qi X.-Q., Zhang S.-D., Jiang T, Li X.-G., Chen Y., Santini C.-L., Zhou H., Chou I.M. and Wu L.-F. (2018) High hydrostatic pressure inducible trimethylamine N-oxide reductase improves the pressure tolerance of piezosensitive bacteria Vibrio fluvialis. Front Microbiol. 8:2646. doi: 10.3389/fmicb.2017.02646

19.       Teng Z. J., Zhang W. Y., Chen Y. R., Pan H. M., T. Xiao and Wu L.-F. (2017) Characterization of dominant giant rod-shaped magnetotactic bacteria from a low tide zone of the China Sea. Chinese Journal of Oceanology and Limnology. DOI: doi: 10.1007/s00343-018-7072-2.

20.       Liu J., Zhang W., Li X.-G. Li X., Chen X., Li J.H., Teng Z.J., Xu C., Santini C.L., Zhao L., Zhao Y., Zhang H., Zhang W.-J., Xu K.D., Li C.L., Pan Y.X., Xiao T., H.M. Pan and Wu L.-F. (2017) Bacterial community structure and novel species of magnetotactic bacteria in sediments from a seamount in the Mariana volcanic arc. Scientific Rep. 7(1): 17964. doi: 10.1038/s41598-017-17445-4.

21.       Sulzenbacher G, Roig-Zamboni V, Lebrun R, Guérardel Y, Murat D, Mansuelle P, Yamakawa N, Quian XX, Vincentelli R, Bourne Y, Wu L-F, Alberto F. (2018) Glycosylate and move! The glycosyltransferase Maf is involved in bacterial flagella formation. Environ Microbiol. 2017 Oct 27. doi: 10.1111/1462-2920.13975.

22.       Leao, P., Y. R. Chen, F. Abreu, M. Wang, W. J. Zhang, K. Zhou, T. Xiao, L. F. Wu*, and U. Lins*. (2017) Ultrastructure of ellipsoidal magnetotactic multicellular prokaryotes depicts their complex assemblage and cellular polarity in the context of magnetotaxis. Environ. Microbiol. 19(6):2151-2163.

23.       Ji B., Zhang S.-D., Zhang W.-J., Rouy Z., Alberto F., Santini C.-L., Mangenot S., Gagnot S., Philippe N., Pradel N., Zhang L., Tempel S., Li Y., Médigue C., Henrissat B., Coutinho P.M., Barbe V., E Talla*, and L.-F. Wu* (2017). The chimeric nature of the genomes of marine magnetotactic coccoid-ovoid bacteria defines a novel group of Proteobacteria. Environ. Microbiol. 19(3): 1103-1119.

24.       Chen C., Chen L., Wang P., Wu L.-F. and Song T. (2017). Magnetically-induced elimination of Staphylococcus aureus by magnetotactic bacteria under a swing magnetic field. Nanomedicine: Nanotechnology, Biology and Medicine. 13(2):363-370.

25.       Du H.J., Zhang R., Zhang W.Y., Xu C., Chen Y.R, Pan H.M., Zhou K., Wu L.-F., and T. Xiao (2017) Characterization of uncultivated magnetotactic bacteria from the sediments of Yuehu Lake, China Acta Oceanologica Sinica 36(2):94-104.

26.       Chen L.J., Chen C., Wang P., Chen C., Wu L.-F. and Song T. (2017) A compound magnetic field generating system for targeted killing of Staphylococcus aureus by magnetotactic bacteria in a microfluidic chip. Journal of Magnetism and Magnetic Materials, 427: 90-94.

27.       Zhang S.-D., C.-L. Santini, W.-J. Zhang, V. Barbe, S. Mangenot, C. Guyomar, M. Garel, H. Chen, X. Li, Q.-J. Yin, Y. Zhao, J. Armengaud, J.-C. Gaillard, S. Martini, N. Pradel, C. Vidaud, F. Alberto, C. Médigue, C Tamburini and L.-F. Wu*. (2016) Genomic and physiological analysis reveals versatile metabolic capacity of deep-sea Photobacterium phosphoreum ANT-2200. Extremophiles. 20 (3): 301-310.

28.       Chen C., Chen L., Yi Y., Chen C., Wu L.-F. and Song T*. (2016). Killing of Staphylococcus aureus via magnetic hyperthermia mediated by magnetotactic bacteria. Appl. Environ. Microbiol. 82 (7): 2219-2226.

29.       Dong Y., Li J., Zhang W., Zhang W., Zhao Y., Xiao T., Wu L.-F.  and Pan H. (2016). The detection of magnetotactic bacteria in deep sea sediments from the east Pacific Manganese Nodule Province. Environ Microbiol Rep. 8 (2): 239-249.

30.       Chen Y.R., Zhang W.Y., Zhou K., Pan H.M., Du H., Xu C., Xu J., Pradel N., Santini C.-L., Li J.H., Huang H., Pan Y.X., Xiao T.*  and Wu L.-F.*. (2016) Novel species and expanded distribution of ellipsoidal multicellular magnetotactic prokaryotes. Environ Microbiol Rep. 8 (2): 218-226.

31.       Murat D, Hérisse M, Espinosa L, Bossa A, Alberto F, Wu L.-F. (2015) Opposite and coordinated rotation of amphitrichous flagella governs oriented swimming and reversals in a magnetotactic spirillum. J Bacteriol. 197(20): 3275-3282.

32.       Fuduche M, Postec A, Davidson S, Chauvin JP, Galès G, Hirschler-Réa A, Ollivier B, Wu LF, Pradel N. (2015) Diversity of magnetotactic bacteria from a French pristine Mediterranean area. Curr Microbiol. 70(4):499-505.

33.       Du H.-J., Chen Y.-R., Zhang R., Pan H.-M., Zhang W.-Y., Zhou K., Wu L.-F. and Xiao T. (2015). Temporal distributions and environmental adaptations of two types of multicellular magnetotactic prokaryote in the sediments of Lake Yuehu, China. Environ Microbiol Rep. 7(3):538-46.

34.       Chen Y.-R., Zhang R., Du H.-J., Pan H.-M., Zhang W.-Y., Zhou K., Li J.-H., Xiao T.* and Wu L.-F.* (2015). A novel species of ellipsoidal multicellular magnetotactic prokaryotes from Lake Yuehu in China. Environ Microbiol. 17(3):637-647.

35.       Lin W., Deng A., Wang Z., Li Y., Wen T., Wu L.-F., Wu M., and Pan Y. (2014). Genomic insights into the uncultured genus “Candidatus Magnetobacterium” in the phylum Nitrospirae. ISME J. 8(12): 2463-2477.

36.       Zhang R., Chen Y.-R., Du H.-J., Zhang W.-Y., Pan H.-M., Xiao T.* and Wu L.-F.* (2014). Characterization and phylogenetic identification of a species of spherical multicellular magnetotactic prokaryotes that produces both magnetite and greigite crystals. Res. Microbiol. 165(7): 481-489.

37.       Zhu X., Ge X., Li N., Wu L.-F., Luo C., Ouyang Q., Tu Y. and Chen G. (2014) Angle sensing in magnetotaxis of Magnetospirillum magneticum AMB-1. Integr Biol (Camb). 6(7):706-713.

38.       Zhang S.-D., V. Barbe, M. Garel, W.-J. Zhang, H. Chen, C.-L. Santini, D. Murat, H. Jing, Y. Zhao, A. Lajus, S. Martini, N. Pradel, C. Tamburini and L.-F. Wu* (2014). Genome sequence of luminous piezophilic Photobacterium phosphoreum ANT-2200. GenommeA, 2(2). pii: e00096-14. doi: 10.1128/genomeA.00096-14.

39.       Zhang W.J., Y. Li, L.-F. Wu* (2014) Complex composition and exquisite architecture of bacterial flagellar propellers (in Chinese). Chin Sci Bull. 59(20):1912-1918.

40.       Song T., H.-M. Pan, Z. Wang, T. Xiao and L.-F. Wu (2014) Assessing bacterial magnetotactic behavior by using permanent magnet blocks. Chin Sci Bull, 59(17): 1929-1935.

41.       Song T, Zhao L Z, Wu L.-F. (2014). A method for quantitative determination of the number of magnetosomes in magnetotactic bacteria by a spectrophotometer, IEEE Transactions on Magnetics, 50(11):1-4.

42.       Chen C Y, Chen C F, Yi Y, Chen L J, Wu L.-F, Song T. (2014). Construction of a microrobot system using magnetotactic bacteria for the separation of Staphylococcus aureus. Biomed Microdevices. 16(5):761-770.

43.       Zhang S.D., S. Cargou, N. Petersen, W.J. Zhang, J. Ruan, D. Murat, C.-L. Santini, T. Kato, P. Notareschi, T. Song, Y. Li, K. Namba, A.-M. Gué, and L.-F. Wu* (2014) Swimming behaviour and magnetotaxis function of the marine bacterium strain MO-1. Environment. Microbiol. Rep. 6(1): 14-20.

44.       Lin, W., D. A. Bazylinski, T. Xiao, L. F. Wu, Y. Pan (2014). Life with compass: diversity and biogeography of magnetotactic bacter

45.       Lefèvre, C. T. and L.-F. Wu (2013). Evolution of the bacterial organelle responsible for magnetotaxis. Trends Microbiol. 21: 534-543.

46.       Ji B., Zhang S.-D., Arnoux P., Rouy Z., Alberto F., Philippe N., Murat D., Zhang W.-J., Rioux J.-B., Ginet N., Sabaty M., Mangenot S., Pradel N., Tian J., Yang J., Zhang L., Zhang W.-Y., Pan H., Henrissat B., Coutinho P.M., Li Y., Xiao T., Médigue C., Barbe V., Pignol D., Talla1 E.,* and Wu, L.-F.* (2013). Comparative genomic analysis provides insights into the evolution and niche adaptation of marine Magnetospira sp. QH-2 strain. Environment. Microbiol. 16(2):525-44.

47.       Xu Y, Xu X, Lan R, Xiong Y, Ye C, Ren Z, Liu L, Zhao A, Wu L.-F. and Xu J. (2013). An O-island 172 encoded RNA helicase regulates the motility of Escherichia coli O157:H7. PLoS One. 13;8(6):e64211.

48.       Zhang W.-Y., Zhou K., Pan H.-M., Du H.-J., Chen Y.-R., Zhang R., Wanneng Y., Lu C., Xiao T.* and Wu L.-F.* (2013). Novel rod-shaped magnetotactic bacteria belonging to Alphaproteobacteria. Appl. Environment. Microbiol. 79(3): 3137-3140.

49.       Zhou, K., Zhang, W.-Y., Pan, H.-M., Zhang, S.-D., Li, J.H., Yue, H.-D., Xiao, T. and Wu, L.-F.* (2013). Adaptation of spherical multicellular magnetotactic prokaryotes to the geochemically variable habitat of an intertidal zone. Environment. Microbiol. 15(5): 1595-1605.

50.       Ruan J., Kato T., Santini C.-L., Miyata T., Kawamoto A., Zhang W.-J., Bernadac A, Wu L.-F. and Namba K. (2012). Architecture of a flagellar apparatus in the fast-swimming magnetotactic bacterium MO-1. Proc. Natl. Acad. Sci. USA. 109(50): 20643-8.

51.       Bernadac A., Wu L.-F*., Santini C.-L., Vidaud C., Sturgis J. N., Menguy N., Bergam P., Nicoletti C. and Xiao T. (2012) Structural properties of the tubular appendage spinae from marine bacterium Roseobacter sp. strain YSCB. Sci. Reports. 2:950

52.       Grimaud R, Ghiglione JF, Cagnon C, Lauga B, Vaysse PJ, Rodriguez-Blanco A, Mangenot S, Cruveiller S, Barbe V, Duran R, Wu L.-F, Talla E, Bonin P, Michotey V. (2012) Genome sequence of the marine bacterium Marinobacter hydrocarbonoclasticus SP17, which forms biofilms on hydrophobic organic compounds. J Bacteriol. 194: 3539-3540.

53.       Zhang W.-Y., Zhou K., Pan H.-M., Yue H., Jiang M, Xiao, T. and Wu, L.-F.* (2012). Two genera of magnetococci with bean-like morphology from intertidal sediments of the yellow sea, China. Appl. Environ. Microbiol. 78: 5606-5611.

54.       Yang C., Chen C., Ma Q., Wu L.-F. and Song T. (2012) Dynamic model and motion mechanism of magnetotactic bacteria with two lateral flagellar bundles. J. Bio. Engin. 9: 200-210.

55.       Ma Q., Chen C., Wei S., Chen C., Wu L.-F. and Song T. (2012) Construction and operation of a microrobot based on magnetotactic bacteria in a microfluidic chip. Biomicrofluidics 6(2): 24107- 24112.

56.       Qi L., Zhang W.-J., Liu J., Rong C., Li Y. and Wu L.-F. (2012) Fur in Magnetospirillum gryphiswaldense influences magnetosomes formation and directly regulates the genes involved in iron and oxygen metabolism. Plos One. 7: e29572.

57.       Zhang W.-J., Santini C.-L., Bernadac A., Ruan J., Zhang S.-D., Kato T., Li Y., Namba K. and Wu L.-F.* (2012) Complex spatial organization and flagellin composition of flagellar propeller from marine magnetotactic ovoid strain MO-1. J. Mol. Biol. 416:558-570.

58.       Zhou, K., Zhang, W.-Y., Yu-Zhang, K., Pan, H.-M., Zhang, S.-D., Zhang, W.-J., Yue, H.-D., Li, Y., Xiao, T. and Wu, L.-F.* (2012). A novel genus of multicellular magnetotactic prokaryotes from the Yellow Sea. Environment. Microbiol., 14: 405-413.

59.       Postec A, Tapia N, Bernadac A, Joseph M, Davidson S, Wu L-F, Ollivier B, Pradel N. (2012) Magnetotactic bacteria in microcosms originating from the French Mediterranean Coast subjected to oil industry activities. Microb Ecol. 63:1-11.

60.       Zhou, K., Pan, H., Zhang, S., Yue, H., Xiao, T. and Wu, L.-F.* (2011). Occurrence and microscopic analyses of multicellular magnetotactic prokaryotes from coastal sediments in the Yellow Sea. Chinese Journal of Oceanology and Limnology 29: 246-251.

61.       Lefèvre C.T, Santini C.-L., Bernadac A., Zhang W.-J., Li Y. and Wu L.-F.* (2010) Calcium-ion mediated assembly and function of glycosylated flagellar sheath of marine magnetotactic bacterium. Mol. Microbiol. 78:1304-1312.

62.       Zhang W.-J., Chen C. Li Y. Song T. and Wu L.-F.* (2010) Configuration of redox gradient determines magnetotactic polarity of the marine bacteria MO-1. Environment. Microbiol. Environment. Microbiol. Rep. 2: 646–650.

63.       Philippe N. and Wu L.-F.* (2010) An MCP-like protein interacts with the MamK cytoskeleton and is involved in magnetotaxis in Magnetospirillum magneticum AMB-1. J. Mol. Biol. 400: 309-322

64.       Zhu K., Pan H., Li J., Yu-Zhang K., Zhang S.D., Zhang W.Y., Zhou K., Yue H., Pan Y., Xiao T. and Wu L.-F.* (2010) Isolation and Characterization of a Marine Magnetotactic Spirillum Axenic Culture QH-2 from Intertidal Zone of the China Sea. Res. Microbiol. 161: 276-283.

65.       Rioux J.-B.,  Philipped N., Pereirae S., Pignol D., Wu L.-F. and Ginet N. (2010) A second actin-like MamK protein in Magnetospirillum magneticum AMB-1 encoded outside the genomic magnetosome island. PlosOne, 5(2): e9151.

66.       Zhang F., Yu-Zhang K., Zhao S., Xiao, T., Denis M. and Wu, L.-F.* (2010) Metamorphosis of Magnetospirillum magneticum AMB-1 cells. Chinese J. Oceanol. Limnol. 28: 304-309

67.       Pan W., Chen C., Wang X.K., Ma Q., Jiang W., Lv J., Wu L.-F. and Song T. (2009). Effects of pulsed magnetic field on the formation of magnetosomes in the Magnetospirillum magneticum AMB-1. Bioelectromagnetics. 31: 246-251.

68.       Zhang L., Zhu Z., Jing H., Zhang J., Xiong Y., Yan M., Gao S., Wu L.-F., Xu J. and Kan B. (2009) Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in Vibrio cholera. BMC Microbiol. 9:114.

69.       Lefèvre C.T, Song T., Yonnet J.P. and Wu L.-F.* (2009). Characterization of bacterial magnetotactic behaviors using a magneto-spectrophotometry assay Appl. Environ. Microbiol. 75: 3835-3841.

70.       Lefèvre C.T, Bernadac A., Yu-Zhang K., Pradel N. and Wu L.-F.* (2009) Isolation and characterization of a magnetotactic bacterial culture from the Mediterranean sea. Environ. Microbiol. 11(7), 1646–1657

71.       Alphandéry, E., Ding, Y., Ngo, A. T., Wang, Z. L., Wu, L. F. and Pileni, M. P. (2009). Assemblies of aligned magnetotactic bacteria and extracted magnetosomes: what is the main factor responsible for the magnetic anisotropy? ACS Nano 3, 1539-1547.

72.       Kikuchi Y., Itaya H., Date M., Matsui K. and Wu L.-F. (2009). TatABC overexpression improves Tat-dependent protein secretion in Corynebacterium glutamicum. Appl. Environ. Microbiol. 75: 603-607.

73.       Pradel N., Delmas J., Wu L.-F., Santini C.-L., and Bonnet R. (2008). Translocation of different β-lactamases across Escherichia coli cytoplasmic membrane. Antimicrobial Agents and Chemotherapy. 53: 242-248.

74.       Alphandéry E., Anh-Tu. N., Lefèvre C., Lisiecki I., Wu , L.-F. and Pileni M.P. (2008) Difference between the magnetic properties of the magnetotactic bacteria and those of the extracted magnetosomes: Influence of the distance between the chains of magnetosomes. J. Phys. Chem. 112: 12304-12309.

75.       Wang X.K., Ma Q.F., Jiang W., Lv J., Song T. and Wu L.-F. (2008). Effects of hypomagnetic field on magnetosome formation of Magnetospirillum magneticum AMB-1. Geomicrobiology Journal. 25: 296 – 303.

76.       Pan, H., Zhu, K., Song, T., Yu-Zhang, K., Lefèvre, C., Xing, S., Liu, M., Zhao, S., Xiao, T. and Wu, L.-F.* (2008) Characterization of a homogeneous taxonomic group of marine magnetotactic cocci within a low tide zone in the China Sea. Environment. Microbiol. 10: 1158-1164

77.       Taoka A., Asada R., Sasaki H., Anzawa K., Wu L.-F., and Fukumori Y. (2007) Polymerization of the actin-like protein MamK, which is associated with magnetosomes. J. Bacteriol. 189:8737-8740.

78.       Kikuchi Y., Itaya H., Date M., Matsui K. and Wu L.-F. (2007). Production of Chryseobacterium proteolyticum protein-glutaminase using the twin-arginine translocation pathway in Corynebacterium glutamicum. Appl. Microbiol. Biotechnol. 78: 67-74.

79.       Lefèvre C., Bernadac A., Pradel N., Wu L.-F., Y.-Zhang K., Xiao T., Yonnet J-P, Lebouc A., Song T. and Y. Fukumori (2007) Characterization of Mediterranean magnetotactic bacteria. J. Ocean Univ. China 6 :355-359.

80.       Perez-Rodriguez R., Fisher A.C., Perlmutter J.D., Hicks M. G., Chanal A., Santini C.-L., Wu L.-F., Palmer T. and. DeLisa M.P. (2007) An essential role for the DnaK molecular chaperone in stabilizing overexpressed substrate proteins of the bacterial twin-arginine translocation pathway. J. Mol. Biol. 3: 715-730.

81.       Xiong Y., Santini C.-L., Kan B., Xu J., Filloux A. and Wu L.-F.* (2007) Expression level of heterologous tat genes is crucial for in vivo reconstitution of a functional Tat translocase in Escherichia coli. Biochimie. 89: 676-85.

82.       Zhao L., Wu D., Wu L.-F. and Song T. (2007). A simple and accurate method for quantification of magnetosomes in magnetotactic bacteria by common spectrophotometer. J. Biochem. Biophys. Methods 70: 377-383.

83.       Pradel, N., Santini, C.-L., Bernadac, A., Shih, Y.-L., Goldberg, M. B., and Wu L.-F. (2007) Periplasmic space and polar positional information in Escherichia coli spherical cells. Biochem. Biophy. Res. Commun. 353: 493-500.

84.       Pradel N., Santini C.-L., Bernadac A., Fukumori Y. and Wu L.-F. (2006) Biogenesis of actin-like bacterial cytoskeletal filaments destined for positioning prokaryotic magnetic organelles. Proc. Natl. Acad. Sci. USA 103:17485-17489.

85.       Kikuchi Y., Date M., Itaya H., Matsui K. and Wu L.-F. (2006). Functional analysis of the twin-arginine translocation pathway in Corynebacterium glutamicum ATCC13869. Appl. Env. Microbiol. 72:7183-7192..

86.       Gao J, Pan H, Xiao T, Barbier G, Wang Z, Yue H, Sun S, Nitsche S, Bernadac A, Pradel N, Wu L.-F.* (2006) Isolation and characterization of novel marine Roseobacter clade members producing unique intracellular chromium-rich aggregates. Res. Microbiol. 157: 714-719.

87.       Taoka A., Asada R., Sasaki H., Anzawa K., Wu L.-F., and Fukumori Y. (2006) Spatial Localizations of Mam22 and Mam12 in the Magnetosomes of Magnetospirillum magnetotacticum. J. Bacteriol. 188:3805-12.

88.       Zhang F, Chen J, Chi Z and Wu L-F.* (2006) Expression and processing of Vibrio anguillarum zinc-metalloprotease in Escherichia coli. Arch Microbiol. 186:11-20.

89.       Chen M., Xie K., Yuan J., Yi L., Facey S.J., Pradel N., Wu L.-F., Kuhn A. and Dalbey R.E. (2005) Involvement of SecDF and YidC in the membrane insertion of M13 procoat mutants. Biochemistry. 44: 10741-10749.

90.       Pradel N., Decorps A., Ye C-Y, Santini C.-L. and Wu L.-F.* (2005) YidC-dependent translocation of green fluorescence protein fused to the FliP cleavable signal peptide. Biochimie. 87: 191-196.

91.       Gérard F., Pradel N. and Wu L.-F.* (2005) Bactericidal activity of colicin V is mediated by an inner membrane protein SdaC of Escherichia coli. J. Bacteriol. 187: 1945-1950.

92.       Pradel N., Ye C-Y and Wu L.-F. (2004) A cleavable signal peptide is required for the full function of the polytopic inner membrane protein FliP of Escherichia coli. Biochem. Biophys. Res. Commun. 319: 1276-1280.

93.       Gérard F., Pradel N., Ye C-Y, Ize B., Yi L., Xu J. Dalbey R.E. and Wu L.-F. (2004) Putative membrane assembly of EtpM-colicin V chimeras. Biochimie. 86: 283-286.

94.       Gouffi K., Gérard F., Santini C-L. and Wu L.-F. (2004) Dual topology of the Escherichia coli TatA protein. J. Biol. Chem. 279: 11608-11615.

95.       Pradel N., Ye C-Y, Livrelli V., Xu J., Joly B. and Wu L.-F. (2003) Contribution of the TAT system to the virulence of enterohemorrhagic Escherichia coli O157:H7. Infect. Immun. 71: 4908-4916.

96.       Zhang M., Pan R.R., Yu Z.L., Wu L.-F. (2003) Assessment of the Escherichia coli Tat Protein Translocation System with Fluorescent Proteins. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 35(8):702-706 (in Chinese)

97.       Zhang M., Pradel N., Mandrand-Berthelot M.-A., Yu Z. and Wu L.-F. (2003) Effect of alteration of the C-terminal extension on the maturation and folding of the large subunit of the Escherichia coli hydrogenase-2. Biochimie. 856: 574-578.

98.       Pradel N., Santini C.-L., Ye C-Y, Fevat L., Gérard F., Alami M. and Wu L.-F. (2003) Influence of tat mutations on the ribose-binding protein translocation in E. coli. Biochem. Biophys. Res. Commun. 306:786-791.

99.       Chanal A., Santini C.L. and Wu L.-F. (2003) Specific inhibition of the translocation of a subset of Escherichia coli TAT substrates by the TorA signal peptide. J. Mol. Biol. 327:563-570.

100.     Ize B., Gérard F., Zhang M., Chanal A., Voulhoux R., Palmer T., Filloux A. and Wu L.-F. (2002) In vivo dissection of the Tat translocation pathway in Escherichia coli. J. Mol. Biol. 317: 327-335.

101.     Ize B., Gérard F. and Wu L.-F. (2002) In vivo assessment of the Tat signal peptide specificity in Escherichia coli. Arch. Microbiol. 178:548-533.

102.     Gouffi K., Santini C.L. and Wu L.-F. (2002) Topology determination and functional analysis of the Escherichia coli TatC protein. FEBS Lett. 525: 65-70.

103.     Alami M., Trescher D., Wu L.-F. and Muller M. (2002) Separate analysis of Tat-specific membrane-binding and translocation in Escherichia coli. J. Biol. Chem. 277: 20499-20503.

104.     Gérard F., Angelini S. and Wu L.-F. (2002) Export of Thermus thermophilus cytoplasmic β-glycosidase via the E. coli Tat pathway. J. Mol. Microbiol. Biotechnol. 4:533-538.

105.     Filloux A. Voulhoux R., Ize B., Gérard F., Ball G. and Wu L.-F. (2002) Use of colicin-based genetic tools for studying bacterial protein transport. Biochimie. 84:489-497.

106.     Yen, M. R., Tseng, Y. H., Nguyen, E. H., Wu, L.- F. and Jr Saier, M. H. (2002) Sequence and phylogenetic analyses of the twin-arginine targeting (Tat) protein export system. Arch. Microbiol. 177: 441-450.

107.     Zhang, L. J., Gao, S. Y., Jing, H. Q., Kan, B., Qi, G. M., Liu, Y. Q., Wu, L. -F., and Xu, J.G. (2002) Analysis of gene cluster of Tat-dependent protein export system of Vibrio cholerae and its function. Yi Chuan Xue Bao 29: 936-940 (en chinois).

108.     Santini C.L., Bernadac A., Zhang M., Chanal A., Ize B., Blanco C. and Wu L.-F. (2001) Translocation of jellyfish green fluorescent protein via the Tat system of Escherichia coli and change of its periplasmic localization in response to osmotic up-shock. J. Biol. Chem. 276: 8159-8164.

109.     Angelini, S., Moreno, R., Gouffi, K., Santini, C.-L., Yamagishi, A., Berenguer, J. and Wu, L.-F. (2001) Export of Thermus thermophilus alkaline phosphatase via the Twin-arginine translocation pathway in Escherichia coli. FEBS Lett. 506:103-107.

110.     Laurinavichene T.V., Chanal A., Wu L.-F. and Tsygankov A.A. (2001) Effect of O2, H2 and redox potential on the activity and synthesis of hydrogenase 2 in Escherichia coli. Res. Microbiol. 152:793-798.

111.     Voulhoux R., Ball G. Ize B., Vasil M.L., Lazdunski A., Wu L.-F. and Filloux A. (2001) Involvement of the twin-arginine translocation system in protein secretion via the type II pathway. EMBO J. 20: 6735-6741.

112.     Beck K., Wu L.-F., Brunner J. and Muller M. (2000) Discrimination between SRP- and SecA/SecB-dependent substrates involves selective recognition of nascent chains by SRP and trigger factor. EMBO J. 19 134-143.

113.     Bourot S., Sire O., Trautwetter A., Touze T., Wu L.-F., Blanco C. and Bernard T. (2000) Glycine betaine-assisted protein folding in a lysA mutant of Escherichia coli. J. Biol. Chem. 275:1050-1056.

114.     Wu L.-F., Ize B., Chanal A., Quentin Y. and Fichant G. (2000) Bacterial twin-arginine signal peptide-dependent protein translocation pathway: evolution and mechanism. J. Mol. Microbiol. Biotech. 2:179-189.

115.     Wu L.-F., Chanal A. and Rodrigue A. (2000) Membrane targeting and translocation of bacterial hydrogenases. Arch. Microbiol. 173:319-324.

116.     Buc J., Santini C.-L., Giordani R., Czjzek M., Wu L.-F. and Giordano G. (1999) Enzymatic and physiologic properties of the tungsten-substituted molybdenum TMAO reductase from Escherichia coli. Mol. Microbiol. 32: 159-168.

117.     Mikhaleva N. I., Santini C., Giordano G., Nesmeyanova M. A. and Wu, L.-F. (1999) Requirement for phospholipids of the translocation of the trimethylamine N-oxide reductase through the tat pathway in Escherichia coli. FEBS. Lett. 463: 331-335.

118.     De Pina K., Desjardin V., Mandrand-Berthelot M.A., Giordano G. and Wu L.-F. (1999) Isolation and characterization of the nikR gene encoding a nickel-responsive regulator in Escherichia coli. J. Bacteriol. 181: 670-674

119.     Rodrigue, A., Chanal, A., Beck, K., Muller, M. and Wu, L.-F. (1999) Co-translocation of a periplasmic enzyme complex by a hitchhiker mechanism through the bacterial Tat-pathway. J. Biol. Chem. 274: 13223-13228.

120.     Santini C.-L., Ize B., Chanal A., Muller M., Giordano G. and Wu L.-F. (1998) A novel Sec-independent periplasmic protein translocation pathway in Escherichia coli. EMBO J. 17 : 101-112.

121.     Chanal A., Santini C.-L and Wu L.-F. (1998) Potential receptor function of three homolgous comonents, TatA, TatB and TatE, of the twin-arginine signal sequence-dependent metalloenzyme translocation pathway in Escherichia coli. Mol. Microbiol. 30: 674-676.

122.     Allan C.B., Wu L.-F., Gu Z., Choudhury S.B., Mandrand-Berthelot M.-A. and Maroney M.J. (1998) An X-ray absorption spectroscopic structural investigation of the nickel site in Escherichia coli NikA protein. Inorg. Chem. 37: 5952-5955.

123.     Rodrigue A., Boxer D.H., Mandrand-Berthelot M.A. and Wu L.-F. (1996) Requirement for nickel of the transmembrane translocation of NiFe-hydrogenase 2 in Escherichia coli. FEBS Lett. 392: 81-86.

124.     Gouesbet G., Trautwetter A., Bonnassie S., Wu L.-F. and Blanco C. (1996) Characterization of the Erwinia chrysanthemi osmoprotectant transporter gene ousA. J Bacteriol. 178: 447-455.

125.     Koch S., Sutrina S. L., Wu L.-F., Reizer J., Schnetz K., Rak B. and Saier, M. H. Jr. (1996) Identification of a site in the phosphocarrier protein, HPr, which influences its interactions with sugar permeases of the bacterial phosphotransferase system: kinetic analyses employing site-specific mutants. J. Bacteriol. 178: 1126-1133.

126.     Rodrigue A., Batia N., Müller M., Fayet O., Böhm R., Mandrand-Berthelot M.A. and Wu L.-F. (1996) Involvement of the GroE chaperonins in the nickel-dependent anaerobic biosynthesis of NiFe-hydrogenases of Escherichia coli. J. Bacteriol. 178: 4453-4460.

127.     Wu L.-F and Mandrand-Berthelot M. A. (1995). A family of homologous substrate-binding proteins with broad range of substrate specificity and dissimilar biological functions. Biochimie 77: 744-750.

128.     De Pina K., Navarro C., McWalter L., Boxer D.H., Price N., Kelly S. Mandrand-Berthelot M.A. and Wu L.-F. (1995) Purification and characterization of the periplasmic nickel binding protein NikA of Escherichia coli K-12. Eur. J. Biochem. 227, 857-865

129.     Wu L.-F., Navarro C., De Pina K., Quenard M. and Mandrand M.A. (1994). Antagonistic effect of nickel on the fermentative growth of Escherichia coli K-12 and comparison of nickel and cobalt toxicity on the aerobic and anaerobic growth. Environmental Health Perspectives. 102, 297-300.

130.     Charon M.-H, Wu L.-F., Florez-Belakbovsky I., de Pina K., Mandrand-Berthelot M.A. and Fontecilla-Camps J.C. (1994). Crystallization and preliminary X-ray study of the nickel-binding protein NikA of Escherichia coli. J. Mol. Biol. 243, 353-355.

131.     Gouesbet G., Abaibou H., Wu L.-F., Mandrand-Berthelot M.A. and Blanco C. (1993). Osmotic repression of anaerobic metabolic systems in Escherichia coli. J. Bacteriol. 175, 214-221.

132.     Wu L.-F. and Mandrand M.A. (1993). Microbial hydrogenases : primary structure, classification, signatures and phylogeny. FEMS Microbiol. Rev. 104, 243-270.

133.     Navarro C., Wu L.-F. and Mandrand-Berthelot M.A. (1993). The nik operon of Escherichia coli encodes a periplasmic binding protein-dependent transport system for nickel. Mol. Microbiol. 9, 1181-1191.

134.     Wu L.-F. (1992). Nickel binding sites in procaryotic proteins. Res. Microbiol. 143 : 347-351.

135.     Reizer J., Sutrina S.L., Wu L.-F., Deutscher J. and Saier M.H. Jr. (1991). Functional interactions between proteins of the phosphoenolpyruvate:sugar phosphotransferase systems of Bacillus subtilis and Escherichia coli. J. Biol. Chem. 267 : 9158-9169.

136.     Wu L.-F., Reizer A., Reizer J., Cai B., Tomich J.M. and Saier M.H.Jr. (1991). Nucleotide sequence of the fruK gene of Rhodobacter capsulatus encoding fructose-1-phosphate kinase : evidence for a kinase superfamily including both phospho-fructose kinases of E. coli. J. Bacteriol. 173 : 3117-3127.

137.     Pao G.M., Wu L.-F., Johnson K.D., Hoft H., Chrispeels M.J., Sweet G., Sandal N.N. and Saier M.H.Jr. (1991). Evolution of the MIP family of integral membrane transport proteins. Mol. Microbiol. 5 : 33-37.

138.     Yamada Y., Chang Y.Y., Daniels G.A., Wu L.-F., Tomich J.M., Yamada M. and Saier M.H.Jr. (1991). Insertion of the mannitol permease into the membrane of Escherichia coli : possible involvement of an N-terminal amphiphilic sequence. J. Biol. Chem. 266 : 17863-17871.

139.     Vartak N.B., Reizer J., Reizer A., Gripp J.T., Groisman.A., Wu L.-F., Tomich J.M. and Saier M.H.Jr. (1991). Sequence and evolution of the FruR protein of Salmonella typhimurium : a pleiotropic transcriptional regulatory protein possessing both activator and repressor functions which is homologous to the periplasmic ribose binding protein. Res. Microbiol. 142 : 951-963.

140.     Wu L.-F. and Saier M.H.Jr. (1991). Differences in codon usage among genes encoding proteins of different functions in Rhodobacter capsulatus. Res. Microbiol. 142  : 943-949.

141.     Wu L.-F., Navarro C. and Mandrand M.A. (1991). The hydC region contains a multicistronic operon (nik) involved in nickel metabolism in Escherichia coli. Gene 107 : 37-42.

142.     Wu L.-F. and Saier M.H.Jr. (1990). On the evolutionary origins of the bacterial phosphoenol-pyruvate:sugar phosphotransferase system. Mol. Microbiol. 4 : 1219-1222.

143.     Saier M.H.Jr., Wu L.-F. and Reizer J. (1990). Regulation of bacterial physiological processes by three types of protein phosphorylating systems. Trends Biochem. Sci. 15 : 391-395.

144.     Wu L.-F. and Saier M.H.Jr. (1990). Nucleotide sequence of the fruA gene encoding the fructose permease of the phosphotransferase system from Rhodobacter capsulatus and analyses of the deduced protein sequence. J. Bacteriol. 172 : 7167-7178.

145.     Wu L.-F., Tomich J.M. and Saier M.H.Jr. (1990). Structure and evolution of a multidomain, multiphosphoryl transfer protein : nucleotide sequence of the fruB(HI) gene in Rhodobacter capsulatus and comparisons with homologous genes from other organisms. J. Mol. Biol. 213 : 687-703.

146.     Jiang W., Wu L.-F., Tomich J., Saier M.H.Jr. and Neihaus W.G. (1990). Revised sequence of the mannitol (MTL) operon in Escherichia coli. Mol. Microbiol. 4 : 2003-2006.

147.     Saier M.H.Jr., Wu L.-F., Baker M.E., Sweet J., Reizer A. and Reizer J. (1990). Evolution of permease diversity and energy coupling mechanisms with special reference to the bacterial phosphotransferase system. Biochim. Biophys. Acta 1018 : 248-251.

148.     Wu L.-F., Mandrand-Berthelot M.A., Waugh R., Edmonds C.J., Holt S.E. and Boxer D.H. (1989). Nickel deficiency gives rise to the defective hydrogenase phenotype of hydC and fnr mutants in Escherichia coli . Mol. Microbiol. 3 : 1709-1718.

149.     Wu L.-F. and Mandrand-Berthelot M.A. (1987). Regulation of the fdhF gene encoding the selenopolypeptide for benzyl viologen-linked formate dehydrogenase in Escherichia coli. Mol. Gen. Genet. 209 : 129-134.

150.     Wu L.-F. and Mandrand-Berthelot M.A. (1987). Characterization of the product of the cloned fdhF gene of Escherichia coli . J. Gen. Microbiol. 133 : 2421-2426.

151.     Wu L.-F. and Mandrand-Berthelot M.A. (1986). Genetic and physiological characterization of new Escherichia coli mutants impaired in hydrogenase activity. Biochimie 68 : 167-179.

152.     Wu L.-F. and Mandrand-Berthelot M.A. (1986). Molecular cloning of the fdhF gene of Escherichia coli K-12. FEMS Microbiol. Lett. 34 : 323-327.

1984-1988  Genetic and physiological study of the formate-hydrogen lyase system of Escherichia coli.

1988-1990  Molecular and biochemical study of bacterial phosphoenol-pyruvate:sugar phosphotransferase system

1990-1995  Nickel metabolism in Escherichia coli.

1995-2003  Translocation of folded proteins in microorganisms.

2003-2019  Magnetotactic bacteria and mechanism of magnetotaxis

2017-          Photobiology of marine microorganisms

​

·             ‘Active’-‘Passive’ Magnetotaxis and Photosensitive magnetoreception. 8^th conference of magnetotactic microorganisms, 2019/10/18-21, Qingdao, China.

·             Photosensitive magnetoreception of marine bacteria. 8^th International conference on magneto-science, 2019/10/11-14, Hefei, China.

·             Photobiology of marine microorganisms. Summer Camp of Institute of Deep-Sea Science and Engineering, 2019/07/21, Sanya, China.

·             Bacterial swimming: Beyond the chemotaxis paradigm. Microbiology at a glance. 2019/4/4-5. IMM, CNRS, Marseille, France.

·             Fascinating features of magnetic multicellularity. The 13th annual meeting of the International Conference on Genomics (ICG-13). 2018/10/25-28. Shenzhen, China

·             Paradigm and paradox of photobiology in the dark oceans. 2017 Winter School of Frontier and Interdisciplinary sciences for the Oversea Students. 2018/01/15-19, Sanya, China.

·             Electromagnetic effect on magnetotaxis of magnetoglobules. 7th workshop on Magnetotactic bacteria and applications. September 22-25, 2017, Huaibei, China.

·             Paradox of photobiology in the dark deep oceans. International Conference on Genomics Ocean, September 7-8 2017, Qingdao, China.

·             Marine photobiology: from photosensitive magnetotaxis to bio-luminescence. Aoshan International symposium on Deep-Sea ecosystems: Extremophiles and life process. August 29-30 2017, Qingdao, China.

·             Occurrence and characteristics of magnetotactic bacteria in seamount habitats. 5^th International Workshop on Deep-Sea Microbiology. September 10-11 2016, Kyoto, Japan.

·       Magnetotactic bacteria: designed to live at oxic-anoxic interface. Les écosystème anaérobies, VIème Journées thématiques de l’Association Francophone d’Ecologie Microbienne. 31 mai-1 juin 2016. Marseille, France.

·       Magnetotaxis and progress in cultivation of magnetotactic multicellular prokaryote. The 6th symposium on research and application of magnetotactic bacteria and biomineralization. Wuxi, China, 12-14 July 2015.

·       Flagella and motility of magnetotactic bacteria. 4th International Meeting on Magnetotactic Bacteria, Rio de Janeiro, Brazil, September 15-18, 2014.

·       Genomic and physiological analysis of deep sea luminous, piezophilic Photobacterium phosphoreum ANT-2200. 4^th International workshop on Deep Sea Microbiology. Brest, France, September 15-17, 2014

·       Genomic and physiological analysis reveals features of deep-sea adaptation of luminous, piezophilic Photobacterium phosphorum. 10^th strait Ocean Forum. Taipei， Taiwan. April 28- May 1st, 2014.

·       Genomic analyses shed light on the evolution and adaptation of magnetotactic bacteria. The 5th symposium on research and application of magnetotactic bacteria and biomineralization. Fuzhou, China, 26-28 August 2013.

·       Magnetotaxis: Apparatus and Mechanism. 2012 General Meeting of Chinese Society for Microbiology, Nanjing, China, 27-30 October 2012.

·       Characterization of magnetotactic behavior by using magnetodrome and micro-channels. Softflow 2012 - Biological Complex Fluids, Cargese, France, June 25th - July 7^th 2012.

·       Flagellar propeller apparatus and motility of magneto-ovoid strain MO-1. 3^rd International magnetotactic bacteria meeting. Berkeley, USA, 11-14 June 2012.

·       Deciphering the mechanism of Magnetotaxis. 4th symposium on research and application of magnetotactic bacteria and biomineralization. Beijing, China, 1-4 September 2011.

·       Motility behavior and flagellar organization point to diverse mechanisms of magneto-aerotaxis. International symposium on Magnetotactic Bacteria. Beijing, China, 1-4 September 2010.

·       Toward to understanding of active magneto-aerotaxis mechanism. 3^rd symposium on research and application of magnetotactic bacteria and biomineralization. Beijing, China, July 29-30, 2009

·       Multidisciplinary study of protein localization, membrane targeting and assembly in magnetotactic bacteria. 7^th strait Ocean Forum. Hangzhou, China. August 25-27 2008.

·       Multidisciplinary, innovative approaches underpin the fascinating research on magnetotactic bacteria. 2^nd symposium on research and application of magnetotactic bacteria and biomineralization. Weihai, China, August 20-21 2008.

·       Diversity, metagenomics and application of magnetotactic bacteria. International collaboration workshop on functional genomics of marine organisms. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China, July 2 2008.

·       Biogenesis, function and application of magnetosomes. The 10th International Symposium on Biomineralization, From Centimeter to Nanometer. Lianyungang, China, August 31 - September 4, 2008

·       Multidisciplinary study of protein localization, membrane targeting and assembly in magnetotactic bacteria. Magnetotactic Bacteria. Balatonfüred, Hungary, 9-11 June 2008.

·       Function and cellular localization of Tat machinery. TAT machine meeting. Blair Atholl, Scotland. April 13-14, 2008.

·       Functional assembly of Tat machinery. TAT machine meeting. Athens, Greece, Oct 29-Nov 1^st 2007.

·       Diversity of magnetotactic bacteria and Biogenesis of magnetosomes. Congrès Annuel SFBBM. Ile des Embiez, France, 17-19 Octobre 2007.

·       Current knowledge and prospect in the study of magnetotactic bacteria. 1^st symposium on research and application of magnetotactic bacteria and biomineralization. Qingdao, China., April 26-28 2007.

·       Tat machine of V. cholarae. TAT machine meeting. Groningen, Netherland, March 20-21, 2007.

·       Characterization of an axenic culture of Mediterranean magnetotactic bacteria, 7^th HFSP Magnetosome meeting, Nara, Japon, April 18-19 2007.

·       Magnetotactic bacteria and biomedical application of magnetosomes, 1^st general meeting of National Key Laboratory of Infectious microorganisms, Beijing, China, April 17 2007.

·       Toward obtaining an axenic culture of Mediterranean magnetotactic bacteria, 6^th HFSP Magnetosome meeting, Pinsot, France, November 22-25 2006.

·       Expression level of heterologous tat genes is crucial for in vivo reconstitution of a functional Tat translocase in Escherichia coli, TAT machine meeting, Carry-le-Rouet, 17th November, 2006

·       Mechanism of protein targeting to a bacterial organelle: the magnetosome, Sixth Human Frontier Science Program Awardees Annual Meeting, Paris, France, July 3-5 2006.

·       Structure and function of the novel actin-like MamK filaments, 5^th HFSP Magnetosome meeting, Beijing, China, April 16-18 2006.

·       High level expression and purification of Tat complexes, TAT machine meeting, Dublin, Ireland, 31st March 2006.

·       Tat in pathogens, TAT machine meeting, Cortona, Italy, 9 October 2005.

·       Biodiversity of Mediterranean magnetotactic bacteria, 4^th HFSP Magnetosome meeting, Grenoble, France, October 4-6 2005.

·       Functional and structural analysis of Tat complexes from Gram negative bacteria and proteomic study of role of the Tat system in pathogens; TAT MACHINE Kick-off meeting, Groningen, Netherlands, February 11-13, 2005.

·       Biogenesis of magnetosomes; Cellular and Molecular Biology of Membranes, FEBS/EMBO Advanced Lecture Course, Cargese, Corsica, France, June 20 – July 1^st, 2005.

·       Optimal growth conditions for Magnetospirillum spp.; 3^rd HFSP Magnetosome meeting, KANAZAWA, Japan, April 2-4, 2005.

·       Synthesis and topology of E. coli TatA protein. European Science Foundation Conference, Protein Targeting, Spa, Belgium, 26 September-1 October 2003

·       Proteomic analysis of exported proteins in Escherichia coli, Session: Functional genomics, proteomics and bioinformatics, where do we go? Xth International Congress of Bacteriology and Applied Microbiology, Paris, France, July 27-August 1, 2002.

·       Protein targeting to the Tat pathway, Gordon Research Conferences, Bacterial Cell Surfaces, New London, NH, USA, June 23-28, 2002

·       Mechanism of bacterial Tat system. European Science Foundation Conference, Protein Targeting, Acquafredda di Maratea, Italy, 28 September-3 October 2001

·       Protein translocation and bacterial pathogenicity, "International Conference on Life Science and Clinical Medicine" (LSCM2000), Beijing, China, April 19-1, 2000.

·       Translocation of folded proteins and enzyme complexes through the Tat pathway, VIth International Conference on the Molecular Biology of Hydrogenases, Potsdam, Germany, 5-10 August 2000.

·       Hydrogenase biosynthesis and trans-membrane translocation in E. coli. Vth International Conference on the Molecular Biology of Hydrogenases, Albertville, France, 12-17 juillet 1997.

·       Transmembrane translocation of a moblydoenzyme, TMAO reductase in E. coli. "Metalloproteins and analogues" French Society of Biochemistry and Molecular Biology, Toulouse, France, 20-22 January 1997.

·       Requirement of incorporation of molybdo-cofactor for the translocation of the TMAO reductase of E. coli. Spring meeting of French Society of Biochemistry and Molecular Biology, Paris, 24-26 March 1997.

·       Nickel-binding proteins in E. coli. "The role of Nickel in Biological and Catalytic Chemistry", The Fifth North American Chemical Congress in Cancun, Mexico, 11-15 November 1997.

·       Requirement for nickel of the anaerobic biosynthesis of NiFe-hydrogenases in E. coli. COST ACTION 818 Hydrogenases and Environmental Biotechnology "Regulation of hydrogenase biosynthesis", Berlin-Germany, 12-14 September 1996.

已指导学生

江婷  硕士研究生  070703-海洋生物学  

崔文鹏  硕士研究生  070703-海洋生物学  

戴婕  硕士研究生  070703-海洋生物学  

现指导学生

汤弘智  博士研究生  070703-海洋生物学  

陈红  硕士研究生  070703-海洋生物学  

赵芮  硕士研究生  070703-海洋生物学