hepatitis virus, virus variation, virus-host interaction

09.2005-06.2008     Doctor of microbiology

                                Wuhan Institute of Virology, Chinese  Academy of Sciences

Research Project: the impact of HBsAg amino acid substitution on HBV

 

09.2003-07.2005     Master of Immunology

                                Medical School, Wuhan University

                                Research Project: chemokine and chemokine receptor

 

09.1998-07.2003     Bachelor of Medicine

                                Medical School, Wuhan University

                                Study Clinical Medicine

   

09.2012-                 associate research fellow

                                Wuhan Institute of Virology, Chinese  Academy of Sciences

07.2008-08.2012     assistant research fellow

                                Wuhan Institute of Virology, Chinese  Academy of Sciences

From 2016-10-01 to 2017-01-15, "Searching,reading and writing of scientific papers" for postgraduates

   

1.   Wu C, Deng W, Deng L, Cao L, Qin B, Li S, Wang Y, Pei R, Yang D, Lu M, Chen X. Amino acid substitutions at the positions 122 and 145 of hepatitis B surface antigen (HBsAg) determine the antigenicity and immunogenicity of HBsAg and influence in vivo HBsAg clearance. J Virol. 2012. 86(8):4658. 

2.    Cao L, Wu C*, Shi H, Gong Z, Zhang E, Wang H, Zhao K, Liu S, Li S, Gao X, Wang Y, Pei R, Lu M, Chen X*.Coexistence of Hepatitis B Virus Quasispecies Enhances Viral Replication and the Ability To Induce Host Antibody and Cellular Immune Responses. J Virol. 2014;88(15):8656-66.

3.   Li S, Zhao K, Liu S, Wu C*, Yao Y, Cao L, Hu X, Zhou Y, Wang Y, Pei R, Lu M, Chen X. HBsAg sT123N mutation induces stronger antibody responses specific to HBsAg and HBcAg and influences in vivo HBsAg clearance. Virus Res. 2015;210:119-125.

4.   Wu C, Zhang X, Tian Y, Song J, Yang D, Roggendorf M, Lu M, Chen X. The biological significance of amino acid substitutions in hepatitis B surface antigen for glycosylation, secretion, antigenicity and immunogenicity of HBsAg as well as assembly and replication of HBV. J Gen Virol. 2010, 91 (2):483-92. 

5.  Yin Y, Wu C, Song J, Wang J, Zhang E, Liu H, Yang D, Chen X, Lu M, Xu Y. DNA immunization with fusion of CTLA-4 to hepatitis B virus (HBV) core protein enhanced Th2 type responses and cleared HBV with an accelerated kinetic. PLoS One, 2011;6(7):e22524.

6.  Wu C*, Shi H, Lu M, Xu Y, Chen X. A case of hepatitis B reactivation in an anti-HBs positive, anti-HBc positive non-Hodgkin's lymphoma patient. Virol Sin. 2013;28(1):49-52.

7. Wu C, Shi H, Wang Y, Lu M, Xu Y, Chen X. A Case of Hepatitis B Reactivation due to the Hepatitis B Virus Escape Mutant in a Patient undergoing Chemotherapy. Virol Sin. 2012;27(6):369-72.

8.  Mu J, Zhang Y, Hu Y, Hu X, Zhou Y, Zhao H, Pei R, Wu C, Chen J, Zhao H, Yang K, Oers MM, Chen X, Wang Y. Autographa californica Multiple Nucleopolyhedrovirus Ac34 Protein Retains Cellular Actin-Related Protein 2/3 Complex in the Nucleus by Subversion of CRM1-Dependent Nuclear Export. PLoS Pathog. 2016;12(11):e1005994.      

9. Zhang X, Liu H, Xie Z, Deng W, Wu C, Qin B, Hou J, Lu M. Epigenetically regulated miR-449a enhances hepatitis B virus replication by targeting cAMP-responsive element binding protein 5 and modulating hepatocytes phenotype. Sci Rep. 2016;6:25389.

10.Yin F, Wu Z, Fang W, Wu C, Rayner S, Han M, Deng F, Du R, Liu J, Wang M, Wang H, Ning Q, Hu Z. Resistant mutations and quasispecies complexity of hepatitis B virus during telbivudine treatment. J Gen Virol. 2015;96(11):3302-12.

11. Pan D, Lin Y, Wu W, Song J, Zhang E, Wu C, Chen X, Hu K, Yang D, Xu Y, Lu M. Persistence of the recombinant genomes of woodchuck hepatitis virus in the mouse model. PLoS One. 2015;10(5):e0125658.

12. Wang Y, Zhang Y, Han S, Hu X, Zhou Y, Mu J, Pei R, Wu C, Chen X. Identification of a novel regulatory sequence of actin nucleation promoting factor encoded by Autographa californica multiple nucleopolyhedrovirus. J Biol Chem. 2015;290(15):9533-41.

13. Guo M, Pei R*, Yang Q, Cao H, Wang Y, Wu C, Chen J, Zhou Y, Hu X, Lu M, Chen X*. Phosphatidylserine-Specific Phospholipase A1 Involved in Hepatitis C Virus Assembly through NS2 Complex Formation. J Virol. 2015; 89(4):2367-77.

14. Zhu W, Wu C, Deng W, Pei R, Wang Y, Cao L, Qin B, Lu M, Chen X. Inhibition of the HCV core protein on the immune response to HBV surface antigen and on HBV gene expression and replication in vivo. PLoS One. 2012;7(9):e45146.

15. Zhu W, Pei R*, Jin R, Hu X, Zhou Y, Wang Y, Wu C, Lu M, Chen X*. Nuclear receptor 4 group A member 1 determines hepatitis C virus entry efficiency through the regulation of cellular receptor and apolipoprotein E expression. J Gen Virol. 2014;95(7):1510-21.

16. Xu S, Pei R, Guo M, Han Q, Lai J, Wang Y, Wu C, Zhou Y, Lu M, Chen X. Cytosolic phospholipase A2 gamma is involved in hepatitis C virus replication and assembly. J Virol. 2012 Dec;86(23):13025-37. doi: 10.1128/JVI.01785-12.

17. Qiu J, Qin B, Rayner S, Wu C, Pei R, Xu S, Wang Y，Chen X. Novel Evidence Suggests Hepatitis B Virus Surface Proteins Participate in Regulation of HBV Genome Replication. Virol Sin., 2011, 26 (2):131-138.

18. Zhu W, Chang Y, Wu C, Han Q, Pei R, Lu M, Chen X. Full length hepatitis C virus core protein inhibits the initiation of antigen-specific T- and B-cell immune responses. Clin Vaccine Immunol. 2010:1139-1147.

19. Li Y, Chen J, Wu C, Wang L, Lu M, Chen X. Hepatitis B virus/hepatitis C virus upregulate angiopoietin-2 expression through mitogen-activated protein kinase pathway. Hepatol Res. 2010, 40(10):1022-1033.

20. Li K, Wang Y, Bai H, Wang Q, Song J, Zhou Y, Wu C, Chen X. The putative pocket protein binding site of Autographa californica Nucleopolyhedrovirus BV/ODV-C42 is required for virus-induced nuclear actin polymerization. J Virol. 2010, 84(15):7857-7868.

21. Han Q, Xu C, Wu C, Zhu W, Yang R, Chen X. Compensatory mutations in NS3 and NS5A proteins enhance the virus production capability of hepatitis C reporter virus. Virus Res. 2009,145:63-73.

I have been studying the virus-host (cell) interaction in order to determine molecular mechanism of virus replication and to elucidate the pathogenesis of hepatitis virus such as hepatitis B.

(1) 2016  international meeting on molecular biology of HBV, "The ceruloplasmin inhibits hepatitis B virus virion secretion by targeting large surface protein and middle surface protein", post;

(2) 2015  international meeting on molecular biology of HBV, "Modulation of Hepatitis B Virus Gene Expression, transcription and replication by RNA binding motif protein 24 via interaction with pregenomic RNA", oral presentation ; 

(3) 2010  international meeting on molecular biology of HBV, “Amino Acid Substitutions at Positions 122 and 145 of Hepatitis B Virus Surface Antigen (HBsAg) Determine the Antigenicity and Immunogenicity of HBsAg and Influence In Vivo HBsAg Clearance”，post;   

(4)  2009  international meeting on molecular biology of HBV, “Biological significance of amino acid substitutions in hepatitis B surface antigen (HBsAg) for glycosylation, secretion, antigenicity and immunogenicity of HBsAg and hepatitis B virus replication”，   post;