071010-生物化学与分子生物学
0710J3-生物信息学
0710Z2-计算生物学

酶理性设计，酶工程，生物催化

   

（ 1 ） 一种双酶级联催化合成(R)-3-氨基-1-丁醇的方法, 2021, 第 3 作者, 专利号: CN112852895A

（ 2 ） 醇脱氢酶突变体及其在高效不对称还原制备手性双芳基醇化合物中的应用, 2021, 第 2 作者, 专利号: CN112852770A

（ 3 ） 胺脱氢酶突变体及其在手性胺醇化合物合成中的应用, 2021, 第 3 作者, 专利号: CN112852894A

（ 4 ） 脱乙酰氧头孢菌素C合成酶突变体及其在β-内酰胺抗生素母核合成中的应用, 2021, 第 3 作者, 专利号: CN112852913A

（ 5 ） 一种高通量筛选酮类化合物的检测方法及其在酶筛选中的应用, 2021, 第 5 作者, 专利号: CN112394050A

（ 6 ） 醇脱氢酶突变体及其在手性双芳基醇化合物合成中的应用, 2020, 第 3 作者, 专利号: CN111100851A

（ 7 ） 一种手性双芳基醇化合物的合成方法, 2019, 第 4 作者, 专利号: CN109837317A

（ 8 ） 一种手性2-氨基-1-丁醇的合成方法, 2019, 第 4 作者, 专利号: CN109825538A

（ 9 ） 莱茵衣藻的油脂代谢途径相关基因和蛋白以及基因突变藻株及其应用, 2014, 第 5 作者, 专利号: CN103695445A

   

（1） Biosynthesis of a rosavin natural product in Escherichia coli by glycosyltransferase rational design and artificial pathway construction, METABOLIC ENGINEERING, 2022, 第 2 作者
（2） Whole-cell Biotransformation of Penicillin G by a Three-enzyme Co-expression System with Engineered Deacetoxycephalosporin C Synthase, ChemBioChem, 2022, 通讯作者
（3） Unlocking the stereoselectivity and substrate acceptance of enzymes: Proline induced loop engineering test, Angewandte Chemie International Edition, 2022, 第 1 作者
（4） Biosynthesis of β-lactam nuclei in yeast, Metabolic Engineering, 2022, 通讯作者
（5） Whole-cell biocatalytic synthesis of S-(4-chlorophenyl)-(pyridin-2-yl) methanol in a liquid–liquid biphasic microreaction system, BIORESOURCE TECHNOLOGY, 2021, 第 4 作者
（6） Unlocking the Stereoselectivity and Substrate Acceptance of Enzymes: Proline-Induced Loop Engineering Test, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2021, 第 1 作者
（7） Near-perfect control of the regioselective glucosylation enabled by rational design of glycosyltransferases, GREEN SYNTHESIS AND CATALYSIS, 2021, 第 2 作者
（8） High-Throughput Fluorescence Assay for Ketone Detectionand Its Applications in Enzyme Mining and Protein Engineering, ACS OMEGA, 2020, 第 3 作者
（9） Identification of Absidia orchidis steroid 11 beta-hydroxylation system and its application in engineering Saccharomyces cerevisiae for one-step biotransformation to produce hydrocortisone, METABOLIC ENGINEERING, 2020, 第 3 作者
（10） Identification of Absidia orchidis steroid 11β-hydroxylation system and its application in engineering Saccharomyces cerevisiae for one-step biotransformation to produce hydrocortisone, METABOLIC ENGINEERING, 2020, 第 3 作者
（11） The Crucial Role of Methodology Development in Directed Evolution of Selective Enzymes, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2020, 第 1 作者
（12） Data mining of amine dehydrogenases for the synthesis of enantiopure amino alcohols, CATALYSIS SCIENCE & TECHNOLOGY, 2020, 第 2 作者
（13） 机器学习助力酶定向进化, Machine learning-assisted enzyme directed evolution, 生物学杂志, 2020, 第 2 作者
（14） Identification of a novel cytochrome P450 enzyme that catalyzes the C-2 alpha hydroxylation of pentacyclic triterpenoids and its application in yeast cell factories, METABOLIC ENGINEERING, 2019, 第 5 作者
（15） Statistical Analysis of the Benefits of Focused Saturation Mutagenesis in Directed Evolution Based on Reduced Amino Acid Alphabets, ACS CATALYSIS, 2019, 第 2 作者
（16） Laboratory evolution of an alcohol dehydrogenase towards enantioselective reduction of difficult-to-reduce ketones, BIORESOURCES AND BIOPROCESSING, 2019, 第 1 作者
（17） 工业酶结构与功能的构效关系, Structure-function relationships of industrial enzymes, 生物工程学报, 2019, 第 2 作者
（18） 蛋白质工程：从定向进化到计算设计, Protein engineering:from directed evolution to computational design, 生物工程学报, 2019, 第 1 作者
（19） 2018诺贝尔化学奖：酶定向进化与噬菌体展示技术, The Nobel Prize in Chemistry 2018: the directed evolution of enzymes and the phage display technologies, 生物学杂志, 2019, 第 1 作者
（20） Utility of B-Factors in Protein Science: Interpreting Rigidity, Flexibility, and Internal Motion and Engineering Thermostability, CHEMICAL REVIEWS, 2019, 第 3 作者
（21） Conformational Dynamics-Guided Loop Engineering of an Alcohol Dehydrogenase: Capture, Turnover and Enantioselective Transformation of Difficult-to-Reduce Ketones, ADVANCED SYNTHESIS & CATALYSIS, 2019, 第 2 作者
（22） Identification of a novel cytochrome P450 enzyme that catalyzes the C-2α hydroxylation of pentacyclic triterpenoids and its application in yeast cell factories, METABOLIC ENGINEERING, 2019, 第 5 作者
（23） Computational Insights into the Catalytic Mechanism of Bacterial Carboxylic Acid Reductase, JOURNAL OF CHEMICAL INFORMATION AND MODELING, 2019, 第 1 作者
（24） Computer-assisted engineering of the catalytic activity of a carboxylic acid reductase, JOURNAL OF BIOTECHNOLOGY, 2019, 第 1 作者
（25） Biocatalysis of carboxylic acid reductases: phylogenesis, catalytic mechanism and potential applications, GREEN CHEMISTRY, 2018, 第 1 作者
（26） 定向进化技术的最新进展, Recent advances in directed evolution, 生物工程学报, 2018, 第 1 作者
（27） Biosynthesis of dendroketose from different carbon sources using in vitro and in vivo metabolic engineering strategies, BIOTECHNOLOGY FOR BIOFUELS, 2018, 第 3 作者
（28） Methodology Development in Directed Evolution: Exploring Options when Applying Triple-Code Saturation Mutagenesis, CHEMBIOCHEM, 2018, 第 1 作者
（29） The transcriptome landscape of Prochlorococcus MED4 and the factors for stabilizing the core genome, BMC MICROBIOLOGY, 2014, 第 5 作者
（30） Transcriptome analysis of Chlamydomonas reinhardtii during the process of lipid accumulation, GENOMICS, 2013, 第 2 作者

Iterative Saturation Mutagenesis for Semi-rational Enzyme Design, Wiley-VCH, 2021-08, 第 1 作者
In Silico Prediction Methods for Site-Saturation Mutagenesis, Humana, 2022-01, 第 1 作者
Enzyme Engineering: Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science, Wiley-VCH, 2023-01, 第 3 作者

   

（ 1 ） 设计改造羧酸还原酶合成重要医药中间体(S)-2-氨基丁醛的研究, 负责人, 国家任务, 2020-01--2022-12
（ 2 ） 基于环氧水解酶LEH活性中心重构的新反应设计与催化机理研究, 负责人, 国家任务, 2022-01--2025-12