同步辐射技术及应用，生物大分子晶体学

   

082703-核技术及应用
071011-生物物理学

同步辐射光学与技术
生物物理

2011-09--2017-06   中国科学院大学   工学博士
2002-09--2005-06   复旦大学   理学硕士
1998-09--2002-06   东北师范大学   理学学士

   

2005-07~现在, 中国科学院上海应用物理研究所, 晶体学专业组副组长

2020-01-01-今,上海市生物物理学会, 理事

   

[1] 梁淼, 孙波, 王志军, 汪启胜. 一种蛋白质结晶及晶体原位衍射数据采集装置及其采集方法. CN: CN111855718A, 2020-10-30.
[2] 余立, 孙波, 王志军, 汪启胜. 一种同步辐射串行晶体学静电纺丝上样装置以及一种基于同步辐射的串行晶体学实验方法. CN: CN111678929A, 2020-09-18.
[3] 李兆龙, 孙波, 余立, 王志军, 汪启胜. 一种盘式蛋白质结晶板的驱动装置. CN: CN111100182A, 2020-05-05.
[4] 李兆龙, 孙波, 郁峰, 汪启胜. 一种用于自动上样机械手测试的杜瓦结构. CN: CN111103314A, 2020-05-05.
[5] 杨利峰, 汪启胜, 崔莹, 何建华. 一种能高精度地测量蛋白晶体数据的衍射仪. CN: CN106596607B, 2019-02-12.
[6] 杨利峰, 王志军, 汪启胜, 崔莹, 何建华. 用于四刀狭缝的调节装置. 中国: CN106053502B, 2018-11-13.
[7] 崔莹, 孙波, 徐琴, 郁峰, 汪启胜, 何建华. 膜蛋白自动化脂立方相结晶系统. 中国: CN205368542U, 2016.07.06.
[8] 李冰, 黄胜, 汪启胜, 崔莹, 何建华. 同步辐射X光连续衰减装置. 中国: CN205352970U, 2016-06-29.
[9] 杨利峰, 黄胜, 王志军, 汪启胜, 崔莹, 何建华. 蛋白质晶体样品捕获环底座的感应识别装置. 中国: CN205229075U, 2016-05-11.
[10] 黄胜, 汪启胜, 张敏, 何建华. 一种X射线强度衰减器. 中国: CN202486073U, 2012-10-10.

   

[1] Xu, Mengxue, Xu, Qin, Wang, Meitian, Qiu, Shenshen, Xu, Dongqing, Zhang, Weizhe, Wang, Weiwu, He, Jianhua, Wang, Qisheng, Ran, Tingting, Sun, Bo. Crystal structures of TTHA1265 and TTHA1264/TTHA1265 complex reveal an intrinsic heterodimeric assembly. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES[J]. 2022, 207: 424-433, http://dx.doi.org/10.1016/j.ijbiomac.2022.03.020.
[2] 王伟玮, 谢娟娟, 宋吴涛, 刘喜朋, 汪启胜. Sulfolobus acidocaldarius DHH超家族核酸酶Saci0542的酶学特征研究. 微生物学报. 2022, 556-566, https://kns.cnki.net/kcms/detail/detail.aspx?dbcode=CJFD&dbname=CJFDLAST2022&filename=WSXB202202013&v=MDI1NTMzcVRyV00xRnJDVVI3aWZaT1pvRnkvZ1c3N09NajdUYkxHNEhOUE1yWTlFWjRSOGVYMUx1eFlTN0RoMVQ=.
[3] Qin, Jiangping, Zhang, Yan, Zhou, Huan, Yu, Feng, Sun, Bo, Wang, Qisheng. Protein Crystal Instance Segmentation Based on Mask R-CNN. CRYSTALS[J]. 2021, 11(2): https://doaj.org/article/ecf3bd2ca3244ac8b503989baab81002.
[4] Qin, Wei, Yu, Feng, Zhou, Huan, Li, Ping, Zhou, Fang, Li, HuiJin, He, ChunXia, Xing, Lu, Zhou, Xin, Zhao, Dong, Li, PengQuan, Jin, Xi, Wang, QiSheng, He, JianHua, Cao, HuiLing. Complex crystal structure determination and anti-non-small-cell lung cancer activity of the Hsp90(N) inhibitor Debio0932. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY[J]. 2021, 77: 86-97, https://www.webofscience.com/wos/woscc/full-record/WOS:000605200900009.
[5] Li, HuiJin, Wang, QiSheng, Han, Wen, Zhou, Huan, Li, Ping, Zhou, Fang, Qin, Wei, Zhao, Dong, Zhou, Xin, He, ChunXia, Xing, Lu, Li, PengQuan, Jin, Xi, Yu, Feng, He, JianHua, Cao, HuiLing. Anti-NSCLC activity in vitro of Hsp90(N) inhibitor KW-2478 and complex crystal structure determination of Hsp90(N)-KW-2478. JOURNAL OF STRUCTURAL BIOLOGY[J]. 2021, 213(2): http://dx.doi.org/10.1016/j.jsb.2021.107710.
[6] Pan, Yongbing, Du, Jianhui, Liu, Jia, Wu, Hai, Gui, Fang, Zhang, Nan, Deng, Xiaojie, Song, Gang, Li, Yufeng, Lu, Jia, Wu, Xiaoli, Zhan, ShanShan, Jing, Zhaofei, Wang, Jiong, Yang, Yimin, Liu, Jianbang, Chen, Ying, Chen, Qin, Zhang, Huanyu, Hu, Hengrui, Duan, Kai, Wang, Manli, Wang, Qisheng, Yang, Xiaoming. Screening of potent neutralizing antibodies against SARS-CoV-2 using convalescent patients-derived phage-display libraries. CELL DISCOVERY[J]. 2021, 7(1): http://dx.doi.org/10.1038/s41421-021-00295-w.
[7] Zhou, Xuelan, Zhong, Fanglin, Lin, Cheng, Hu, Xiaohui, Zhang, Yan, Xiong, Bing, Yin, Xiushan, Fu, Jinheng, He, Wei, Duan, Jingjing, Fu, Yang, Zhou, Huan, McCormick, Peter J, Wang, Qisheng, Li, Jian, Zhang, Jin. Structure of SARS-CoV-2 main protease in the apo state. SCIENCE CHINA-LIFE SCIENCESnull. 2021, 64(4): 656-659, https://www.webofscience.com/wos/woscc/full-record/WOS:000565828900001.
[8] Yufei Han, Qian Zhuang, Bo Sun, Wenping Lv, Sheng Wang, Qingjie Xiao, Bin Pang, Youli Zhou, Fuxing Wang, Pengliang Chi, Qisheng Wang, Zhen Li, Lizhe Zhu, Fuping Li, Dong Deng, YingChih Chiang, Zhenfei Li, Ruobing Ren. Crystal structure of steroid reductase SRD5A reveals conserved steroid reduction mechanism. NATURE COMMUNICATIONS[J]. 2021, 12(1): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815742/.
[9] Wang, Weiwei, Wu, Hai, Xiao, Qingjie, Zhou, Huan, Li, Minjun, Xu, Qin, Wang, Qisheng, Yu, Feng, He, Jianhua. Crystal structure details of Vibrio fischeri DarR and mutant DarR-M202I from LTTR family reveals their activation mechanism. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES[J]. 2021, 183: 2354-2363, http://dx.doi.org/10.1016/j.ijbiomac.2021.05.186.
[10] Liang, Miao, Wang, Zhijun, Wu, Hai, Yu, Li, Sun, Bo, Zhou, Huan, Yu, Feng, Wang, Qisheng, He, Jianhua. Microplates for Crystal Growth and in situ Data Collection at a Synchrotron Beamline. CRYSTALS[J]. 2020, 10(9): https://doaj.org/article/91ad6294cbd74495b3afa381464cbee6.
[11] Li Wei, Ran Tiecheng, Zhang Yu, He Wei, Ma Jifei, Wang Qisheng, Zhang Jichao, Zhu Ying. SiO2-Mediated High-efficiency Enrichment of 5 nm Gold Nanoparticles and Their Catalytic Activity. ACTA CHIMICA SINICA[J]. 2020, 78(2): 170-176, https://www.webofscience.com/wos/woscc/full-record/WOS:000522755100009.
[12] He, Wei, Peng, Hongzhen, Ma, Jifei, Wang, Qisheng, Li, Aiguo, Zhang, Jichao, Kong, Huating, Li, Qingnuan, Sun, Yanhong, Zhu, Ying. Autophagy changes in lung tissues of mice at 30 days after carbon black-metal ion co-exposure. CELL PROLIFERATION[J]. 2020, 53(7): https://www.webofscience.com/wos/woscc/full-record/WOS:000538894300001.
[13] Du, Shuo, Cao, Yunlong, Zhu, Qinyu, Yu, Pin, Qi, Feifei, Wang, Guopeng, Du, Xiaoxia, Bao, Linlin, Deng, Wei, Zhu, Hua, Liu, Jiangning, Nie, Jianhui, Zheng, Yinghui, Liang, Haoyu, Liu, Ruixue, Gong, Shuran, Xu, Hua, Yisimayi, Ayijiang, Lv, Qi, Wang, Bo, He, Runsheng, Han, Yunlin, Zhao, Wenjie, Bai, Yali, Qu, Yajin, Gao, Xiang, Ji, Chenggong, Wang, Qisheng, Gao, Ning, Huang, Weijin, Wang, Youchun, Xie, X Sunney, Su, Xiaodong, Xiao, Junyu, Qin, Chuan. Structurally Resolved SARS-CoV-2 Antibody Shows High Efficacy in Severely Infected Hamsters and Provides a Potent Cocktail Pairing Strategy. CELL[J]. 2020, 183(4): 1013-+, http://dx.doi.org/10.1016/j.cell.2020.09.035.
[14] Wang, Qihui, Zhang, Yanfang, Wu, Lili, Niu, Sheng, Song, Chunli, Zhang, Zengyuan, Lu, Guangwen, Qiao, Chengpeng, Hu, Yu, Yuen, KwokYung, Wang, Qisheng, Zhou, Huan, Yan, Jinghua, Qi, Jianxun. Structural and Functional Basis of SARS-CoV-2 Entry by Using Human ACE2. CELL[J]. 2020, 181(4): 894-+, http://dx.doi.org/10.1016/j.cell.2020.03.045.
[15] Zhao, FengZhu, Sun, Bo, Yu, Li, Xiao, QingJie, Wang, ZhiJun, Chen, LiangLiang, Liang, Huan, Wang, QiSheng, He, JianHua, Yin, DaChuan. A novel sample delivery system based on circular motion for in situ serial synchrotron crystallography. LAB ON A CHIP[J]. 2020, 20(21): 3888-3898, https://www.webofscience.com/wos/woscc/full-record/WOS:000584044600003.
[16] Wen, Yao, He, Peng, Wang, Qisheng, Yao, Yuyu, Zhang, Yu, Hussain, Sabir, Wang, Zhenxing, Cheng, Ruiqing, Yin, Lei, Sendeku, Marshet Getaye, Wang, Feng, Jiang, Chao, He, Jun. Gapless van der Waals Heterostructures for Infrared Optoelectronic Devices. ACS NANO[J]. 2019, 13(12): 14519-14528, https://www.webofscience.com/wos/woscc/full-record/WOS:000505633300089.
[17] Yu, Feng, Wang, Qisheng, Li, Minjun, Zhou, Huan, Liu, Ke, Zhang, Kunhao, Wang, Zhijun, Xu, Qin, Xu, Chuanyan, Pan, Qiangyan, He, Jianhua. Aquarium: an automatic data-processing and experiment information management system for biological macromolecular crystallography beamlines. JOURNAL OF APPLIED CRYSTALLOGRAPHY[J]. 2019, 52: 472-477, [18] Qisheng Wang, Bo Sun, Huan Zhou, Zhijun Wang, Feng Yu, Jianhua He. An integrated remote data collection system for macromolecular crystallography beamline at SSRF. Nuclear Inst. and Methods in Physics Research, A. 2019, 42-45, http://dx.doi.org/10.1016/j.nima.2018.10.167.
[19] Wang Qisheng. A Level-2 Biosafety Beamline for MacromolecularCrystallography at SSRF.. AIP Conference Proceedings. 2019, [20] 李冰, 汪启胜, 李敏军, 郭豪杰, 余立, 何建华. 基于芯片技术的生物大分子晶体高效数据采集. 核技术[J]. 2019, 42(6): 1-6, http://lib.cqvip.com/Qikan/Article/Detail?id=7002163155.
[21] 张蔚哲, 周欢, 顾亦君, 周晓洁, 李娜, 李怡雯, 汪启胜, 何建华. 上海光源在生命科学中的应用. 现代物理知识. 2019, 31(5): 46-59, http://lib.cqvip.com/Qikan/Article/Detail?id=7100456311.
[22] Wang, Qisheng, Sun, Bo, Zhou, Huan, Wang, Zhijun, Yu, Feng, He, Jianhua. An integrated remote data collection system for macromolecular crystallography beamline at SSRF. NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT[J]. 2019, 914: 42-45, http://dx.doi.org/10.1016/j.nima.2018.10.167.
[23] Wang, QiSheng, Zhang, KunHao, Cui, Yin, Wang, ZhiJun, Pan, QiangYan, Liu, Ke, Sun, Bo, Zhou, Huan, Li, MinJun, Xu, Qin, Xu, ChunYan, Yu, Feng, He, JianHua. Upgrade of macromolecular crystallography beamline BL17U1 at SSRF. NUCLEAR SCIENCE AND TECHNIQUES[J]. 2018, 29(5): https://www.webofscience.com/wos/woscc/full-record/WOS:000433263600008.
[24] Bing Li, Sheng Huang, QiangYan Pan, MinJun Li, Huan Zhou, QiSheng Wang, Feng Yu, Bo Sun, JianQiao Chen, JianHua He. New design for multi-crystal data collection at SSRF. 核技术(英文版). 2018, 29(2): 48-56, http://lib.cqvip.com/Qikan/Article/Detail?id=7000413267.
[25] Li, Bing, Huang, Sheng, Pan, QiangYan, Li, MinJun, Zhou, Huan, Wang, QiSheng, Yu, Feng, Sun, Bo, Chen, JianQiao, He, JianHua. New design for multi-crystal data collection at SSRF. NUCLEAR SCIENCE AND TECHNIQUES[J]. 2018, 29(2): 48-56, http://lib.cqvip.com/Qikan/Article/Detail?id=7000413267.
[26] 钟长游, 汪启胜, 刘科, 张坤浩, 何建华. BL17U短波长X射线聚焦方案优化. 核技术[J]. 2018, 050102-1, [27] Wang Qisheng, Yu Feng, Cui Ying, Zhang Kunhao, Pan Qiangyan, Zhong Changyou, Liu Ke, Zhou Huan, Sun Bo, He Jianhua. Mini-beam modes on standard MX beamline BL17U at SSRF.. The Review of scientific instruments. 2017, http://kns.cnki.net/KCMS/detail/detail.aspx?QueryID=0&CurRec=74&recid=&FileName=SJPD5FF5CCA8D2B32149C73E2B9E99814C8B&DbName=SJPD_06&DbCode=SJPD&yx=&pr=&URLID=&bsm=.
[28] 陈建樵, 汪启胜, 李冰, 周欢, 崔莹, 孙波, 王志军, 何建华. 同步辐射串行晶体学静电纺丝上样实验技术. 核技术[J]. 2017, 40(8): "13-20", [29] Wang, Qisheng, Yu, Feng, Cui, Ying, Zhang, Kunhao, Pan, Qiangyan, Zhong, Changyou, Liu, Ke, Zhou, Huan, Sun, Bo, He, Jianhua. Mini-beam modes on standard MX beamline BL17U at SSRF. REVIEW OF SCIENTIFIC INSTRUMENTS[J]. 2017, 88(7): https://www.webofscience.com/wos/woscc/full-record/WOS:000406773700008.
[30] 汪启胜. SSRF生物大分子光束线站自动化集成及实验技术发展. 2017, http://kns.cnki.net/KCMS/detail/detail.aspx?QueryID=0&CurRec=85&recid=&FileName=1017811030.nh&DbName=CDFDLAST2017&DbCode=CDFD&yx=&pr=&URLID=&bsm=.
[31] Wang Qisheng. Automatic crystal centering procedure at the SSRF macromolecular crystallography beamline. Journal of Synchrotron Radiation.. 2016, [32] 崔莹, 汪启胜, 黄胜, 张坤浩, 李冰, 徐琴, 王志军, Michal Pomorski, 徐慧超, 潘强岩, 何建华. 穿透型金刚石X射线位置灵敏探测器及其在同步辐射光束线上的应用. 核技术[J]. 2016, 39(7): "5-12", [33] Wang, Yuxi, Benz, Frederick W, Wu, Yangping, Wang, Qisheng, Chen, Yunfeng, Chen, Xiangzheng, Li, Huiyan, Zhang, Yonghui, Zhang, Rundong, Yang, Jinliang. Structural Insights into the Pharmacophore of Vinca Domain Inhibitors of Microtubules. MOLECULAR PHARMACOLOGY[J]. 2016, 89(2): 233-242, http://ir.sinap.ac.cn/handle/331007/25843.
[34] Yu, Feng, Li, Minjun, Xu, Chunyan, Sun, Bo, Zhou, Huan, Wang, Zhijun, Xu, Qin, Xie, Muyun, Zuo, Gang, Huang, Pei, Guo, Haojie, Wang, Qisheng, He, Jianhua. Crystal structure and enantioselectivity of terpene cyclization in SAM-dependent methyltransferase TleD. BIOCHEMICAL JOURNAL[J]. 2016, 473: 4385-4397, https://www.webofscience.com/wos/woscc/full-record/WOS:000393760600008.
[35] Wang, Zhijun, Pan, Qiangyan, Yang, Lifeng, Zhou, Huan, Xu, Chunyan, Yu, Feng, Wang, Qisheng, Huang, Sheng, He, Jianhua. Automatic crystal centring procedure at the SSRF macromolecular crystallography beamline. JOURNAL OF SYNCHROTRON RADIATION[J]. 2016, 23: 1323-1332, http://ir.sinap.ac.cn/handle/331007/26436.
[36] 张春波, 汪启胜, 黄胜, 何建华. 同步辐射光束线束斑形状实时分析技术. 核技术[J]. 2015, 38(5): 42294-, http://ir.sinap.ac.cn/handle/331007/24343.
[37] Wang QiSheng, Yu Feng, Huang Sheng, Sun Bo, Zhang KunHao, Liu Ke, Wang ZhiJun, Xu Chunyan, Wang SiSheng, Yang LiFeng, Pan QiangYan, Li Liang, Zhou Huan, Cui Yin, Xu Qin, Earnest, Thomas, He JianHua. The macromolecular crystallography beamline of SSRF. NUCLEAR SCIENCE AND TECHNIQUES[J]. 2015, 26(1): 12-17, http://ir.sinap.ac.cn/handle/331007/24597.
[38] Wang, Feng, Wang, Zhenxing, Wang, Qisheng, Wang, Fengmei, Yin, Lei, Xu, Kai, Huang, Yun, He, Jun. Synthesis, properties and applications of 2D non-graphene materials. NANOTECHNOLOGYnull. 2015, 26(29): https://www.webofscience.com/wos/woscc/full-record/WOS:000357605500004.
[39] Wang, Fengmei, Zhan, Xueying, Cheng, Zhongzhou, Wang, Qisheng, Wang, Zhenxing, Wang, Feng, Xu, Kai, Huang, Yun, Safdar, Muhammad, He, Jun. A High-Energy-Density Asymmetric Microsupercapacitor for Integrated Energy Systems. ADVANCED ELECTRONIC MATERIALS[J]. 2015, 1(4): https://www.webofscience.com/wos/woscc/full-record/WOS:000357654800005.
[40] Wang, Feng, Wang, Zhenxing, Xu, Kai, Wang, Fengmei, Wang, Qisheng, Huang, Yun, Yin, Lei, He, Jun. Tunable GaTe-MoS2 van der Waals p-n Junctions with Novel Optoelectronic Performance. NANO LETTERS[J]. 2015, 15(11): 7558-7566, https://www.webofscience.com/wos/woscc/full-record/WOS:000364725400058.
[41] Wang, Fengmei, Li, Jinshan, Wang, Feng, Shifa, Tofik Ahmed, Cheng, Zhongzhou, Wang, Zhenxing, Xu, Kai, Zhan, Xueying, Wang, Qisheng, Huang, Yun, Jiang, Chao, He, Jun. Enhanced Electrochemical H-2 Evolution by Few-Layered Metallic WS2(1-x)Se2x Nanoribbons. ADVANCED FUNCTIONAL MATERIALS[J]. 2015, 25(38): 6077-6083, https://www.webofscience.com/wos/woscc/full-record/WOS:000362730100009.
[42] Pan, Qiangyan, Wang, Qisheng, Wang, Zhijun, Li, Liang, He, Jianhua. An active beamstop for accurate measurement of high intensity X-ray beams. NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT[J]. 2014, 735: 584-586, https://www.webofscience.com/wos/woscc/full-record/WOS:000327494600078.
[43] 汪启胜, 黄胜, 孙波, 唐琳, 何建华. SSRF生物大分子晶体学线站用户数据采集系统. 核技术. 2012, 35(1): 5-11, http://lib.cqvip.com/Qikan/Article/Detail?id=40533021.
[44] 牛晶, 汪启胜, 王玉, 陶世兴, 黄胜, 何建华, 唐琳. SSRF生物大分子晶体学光束线站多波长反常散射信号采集自动化系统. 核电子学与探测技术. 2012, 32(1): 46-49, http://lib.cqvip.com/Qikan/Article/Detail?id=41480594.
[45] 孙波, 黄胜, 唐琳, 何建华, 陶世兴, 牛晶, 陈鸣之, 刘科, 王玉, 汪启胜. 利用晶体多重衍射进行同步辐射光子能量标定. 光学精密工程. 2011, 19(5): 977-982, http://lib.cqvip.com/Qikan/Article/Detail?id=38123257.
[46] 朱静, 陶世兴, 汪启胜, 黄胜, 何建华. 小型位置灵敏电离室的设计. 核电子学与探测技术[J]. 2010, 30(4): 490-494, http://lib.cqvip.com/Qikan/Article/Detail?id=34119437.
[47] Tao Shixing, Zhu Jing, Niu Jing, Chen Mingzhi, Liu Ke, Wang Yu, Wang Qisheng, Sun Bo, Huang Sheng, Tang Lin, He Jianhua. In-situ energy calibration of SSRF macromolecular crystallography beamline using powder diffraction. NUCLEAR SCIENCE AND TECHNIQUES[J]. 2010, 21(6): 321-324, http://lib.cqvip.com/Qikan/Article/Detail?id=37122670.
[48] 陶世兴, 陈鸣之, 唐琳, 何建华, 朱静, 汪启胜, 黄胜. 上海光源新型一维位置灵敏电离室研制. 光学精密工程[J]. 2010, 18(3): 544-551, http://lib.cqvip.com/Qikan/Article/Detail?id=33300706.

   

（ 1 ） 依托同步辐射光源的蛋白质晶体学前沿关键技术综合研究, 参与, 国家级, 2017-01--2022-12
（ 2 ） 微聚焦X射线对蛋白质晶体辐射损伤的研究, 主持, 省级, 2021-07--2024-06

现指导学生

张晨钰  硕士研究生  071011-生物物理学  

朱智敏  硕士研究生  071011-生物物理学