基本信息
徐卉芳  女  硕导  中国科学院青岛生物能源与过程研究所
电子邮件: xu_hf@qibebt.ac.cn
通信地址: 山东省青岛市崂山区松岭路189号
邮政编码: 266101

研究领域

   

招生信息

   
招生专业
071010-生物化学与分子生物学
071005-微生物学
招生方向
蛋白质工程,酶和生物催化,合成生物学
合成生物学,生物工程

教育背景

1999-09--2002-07   中国科学院微生物研究所   博士
学历
   
学位
   

工作经历

   
工作简历
2015-07~现在, 中国科学院青岛生物能源与过程研究所, 副研究员
2008-08~2015-03,Imperial College London, UK, Senior Research Associate
2004-10~2007-12,Imperial College London, UK, Research Associate
2002-08~2004-10,纽英伦生物技术(北京)有限公司, 研发部主任兼生产部经理
1999-09~2002-07,中国科学院微生物研究所, 博士
社会兼职
2018-04-16-今,EDITORIAL BOARD MEMBER of American Journal of Bioscience and Bioengineering (BIO), EDITORIAL BOARD MEMBER

教授课程

   

专利与奖励

   
奖励信息
(1) 最佳展板奖, 其他, 2018
(2) Best poster prize, The 18th CLSS-UK Annual Meeting. London UK, 其他, 2012
(3) Best poste prize, The XXIInd FECTS Meeting. Davos, Swiss, 其他, 2010
(4) Conference Attendance Award, ICGEB (International Centre for Genetic Engineering and Biotechnology), 其他, 2007
专利成果
( 1 ) Production of Alpha-Olefins, 2016, 第 5 作者, 专利号: WO2017001606 A1
( 2 ) Method for detecting hydrogen peroxide conversion activity of an enzyme, 2019, 第 3 作者, 专利号: WO 2019052907A2
( 3 ) IMPROVED CYTOCHROME P450 FATTY ACID DECARBOXYLASES, 2020, 第 2 作者, 专利号: WO 2020020992 A1

出版信息

   
发表论文
(1) Directed Evolution of P450 Fatty Acid Decarboxylases via High‐Throughput Screening towards Improved Catalytic Activity, ChemCatChem, 2020, 第 1 作者
(2) Establishing an Enzyme Cascade for One-pot Production of α-olefins from Low-cost Triglycerides and Oils without Exogenous H2O2 Addition, Biotechnology for Biofuels, 2020, 第 4 作者
(3) Biochemical characterization of three new α-olefin-producing P450 fatty acid decarboxylases with a halophilic property, Biotechnology for Biofuels, 2019, 第 11 作者
(4) Mutagenesis and redox partners analysis of the P450 fatty acid decarboxylase OleTJE, Scientific Reports, 2017, 第 1 作者
(5) In vitro oxidative decarboxylation of free fatty acids to terminal alkenes by two new P450 peroxygenases, Biotechnology for Biofuels, 2017, 第 1 作者
(6) Complete Elucidation of the Late Steps Bafilomycin Biosynthesis in Streptomyces lohii, Journal of Biological Chemistry, 2017, 第 8 作者
(7) Recombinant Collagen Engineered to Bind to Discoidin Domain Receptors Functions as a Receptor Inhibition, Journal of Biological Chemistry, 2016, 第 3 作者
(8) Molecular and cellular analysis of a novel mutation in DDR2 causing Spondylo-meta-epiphyseal dysplasia with short limbs and abnormal calcifications (SMED-SL), BMC Medical Genetics, 2014, 第 3 作者
(9) Normal activation of discoidin domain receptor 1 mutants with disulfide cross-links, insertions, or deletions in the extracellular juxtamembrane region: mechanistic implications, Journal of Biological Chemistry, 2014, 第 1 作者
(10) Discoidin domain receptors promote alpha1beta1- and alpha2beta1-integrin mediated cell adhesion to collagen by enhancing integrin activation, PLos One, 2012, 第 1 作者
(11) Collagen binding specificity of the discoidin domain receptors: binding sites on collagens II and III and molecular determinants for collagen IV recognition by DDR1, Matrix Biology, 2011, 第 1 作者
(12) Trafficking defects and loss of ligand binding are the underlying causes of all reported DDR2 missense mutations found in SMED-SL patients, Human Molecular Genetics, 2010, 第 1 作者
(13) Directed Evolution of E. coli Alkaline Phosphatase Towards Higher Catalytic Activity, Biocatalysis and Biotransformation, 2003, 第 1 作者
(14) Engineering E. coli Alkaline Phosphatase Yields Changes of Catalytic Activity, Thermal Stability and Phosphate Inhibition, Biocatalysis and Biotransformation, 2002, 第 4 作者
发表著作
   

科研活动

   
科研项目
( 1 ) Improving C12 Activity of New P450 Decarboxylase Encoding Sequences, 主持, 院级, 2015-07--2017-02
( 2 ) 基于P450催化元件的高值天然产物生物合成途径重构, 参与, 省级, 2017-08--2019-07
( 3 ) Proof of Concept of Pathway Integration for α-Olefin Biosynthesis in Photosynthetic Microorganisms, 主持, 院级, 2017-07--2019-07
( 4 ) P450 脂肪酸脱羧酶的定向进化及催化机理研究, 主持, 国家级, 2018-01--2021-12
( 5 ) 耦合非天然辅酶的P450脂肪酸衍生细胞工厂, 主持, 市地级, 2018-03--2020-02
( 6 ) 类囊体膜介导的光驱α-烯烃生物合成研究, 主持, 市地级, 2020-01--2021-12
参与会议
(1)Directed Evolution of P450 Fatty Acid Decarboxylases via High-Throughput Screening towards Improved Catalytic Activity   2019-10-20
(2)催化脂肪酸脱羧生成末端烯烃的新型P450过氧化物酶的鉴定和酶学表征   2018年中国微生物学会学术年会   2018-10-19
(3)Characterization of novel P450 fatty acids decarboxylases for 1-alkene production   第三届合成微生物学与生物制造暨第八届生物催化与生物合成工程国际学术研讨会   2018-10-12
(4)Characterization of novel P450 peroxygenases for oxidative decarboxylation of free fatty acids to terminal alkenes   2018-06-30
(5)学术交流   全国首届生物传感、生物芯片高端论坛   2017-10-20
(6)学术交流   国际化学生物学年会   2017-10-17
(7)学术交流   2016-10-16
(8)Understanding the molecular mechanism for discoidin domain receptor activation   2013-09-01
(9)Discoidin Domain Receptors Promote β1-integrin Mediated Cell Adhesion to Collagen   2012-09-07
(10)The role of discoidin domain receptors in cell adhesion   2012-04-02
(11)The role of DDR Receptors in cell adhesion   2011-09-05
(12)Trafficking defects and loss of ligand binding are the underlying causes of all reported DDR2 missense mutations found in SMED-SL patients   2010-07-03
(13)Determining the collagen binding specificity of the discoidin domain receptors (DDRs)   2010-03-29
(14)Characterization of polyomavirus middle T antigen complexes using affinity purification and MS   2007-07-22

合作情况

   
项目协作单位
   

指导学生