Our research is centered on the design and construction of recombinant microbial bio-systems for the production of complex therapeutic natural products. Current efforts include the isolation and identification of different genes/modules/pathways from various resources, development of tools and methodologies for novel biosynthetic pathway design and the natural products heterologous biosynthesis in the engineered microbes. We particular focus on: 1) elucidating design principles for the production of natural or unnatural natural compounds by using synthetic biology; 2) developing more efficient and economical methods to generate biological products.

Major Research Areas

• Synthetic Biology
• Metabolic Engineering
• Biochemical Engineering
• Bioprocess Engineering

Ph. D - Biochemical Engineering (2004)

School of Biological Engineering,

East China University of Science and Technology, China;

Joint Awarded by Key Laboratory of Biotechnology of Antibiotics, Ministry of Health,

Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, China;

M. S. - Microbial and Biochemical Pharmacy (2001)

Department of Pharmaceutical Biotechnology,

Sichuan Industrial Institute of Antibiotics, SFDA, China;

B. S. - Microbial Engineering (1996)

Department of Microbiology,

Shandong University, China.

Oct 2010-Present     : Professor / Principal Investigator

                           Key Laboratory of Synthetic Biology

                           Shanghai Institutes for Biological Sciences

                           Chinese Academy of Sciences

Sep 2008-Oct 2010 : Professor

State Key Laboratory of Bioreactor Engineering,

School of Biological Engineering,

National Research Center of Biochemical Engineering and Technology,

East China University of Science and Technology

Jan 2005-Aug 2008   : Post Doctorial Fellow, Visiting Researcher

Dept. of Chem. & Biol. Engineering, Tufts University, Medford, USA

Chem. Engineering Dept. MIT, Cambridge, USA

Natural Product Dept., Wyeth Pharmaceuticals, Pearl River, NY

Sep 1996-Jul 1998      :  Assistant Engineer of Bioprocess

Chemical and Light Plant of Bin Zhou, Shandong Province, China

1.     Yang, G., Jia, D., Jin, L., Jiang, Y., Wang, Y., & Jiang, W., et al. (2017). Rapid generation of universal synthetic promoters for controlled gene expression in both gas-fermenting and saccharolytic clostridium species. Acs Synthetic Biology. 6 (9), pp 1672–1678

2.     Shao J, Chen QW, Lv HJ, He J, Liu ZF, Lu YN, Liu HL*, Wang GD*, Wang Y*. (2017) (+)-Thalianatriene and (-)-Retigeranin B Catalyzed by Sesterterpene Synthases from Arabidopsis thaliana. Org Lett. 19 (7):1816–1819

3.     王勇* (2017) 新本草计划—基于合成生物学的药用植物活性代谢物研究, 生物工程学报, 33(3): 478-485

4.     邵洁, 李建华, 王凯博, 王勇*（2017） 植物底盘: 天然产物合成生物学研究的新热点，生物加工过程，9, 15: 24-31

5.      罗杰，王勇，郑亚洁，黄继荣*（2017）植物天然产物与合成生物学, 植物生理学报，8, 20: 1345-1347

6.     杨悦，杨长青，王勇，杨蕾*（2017）过表达丹参 GGPPS 研究丹参酮类的生物合成途径，中国科学：生命科学，5, 20: 586-594

7.     杨玉凤, 费理文, 李建华, 郝千里, 杨靖亚, 王勇*. (2017). 重组大肠杆菌全细胞催化合成莱鲍迪苷D. 工业微生物, 48(5): 1-7.

8.     李微微, 孙雨伟, 杨靖亚, 王勇*. (2017). 甜叶悬钩子(Rubus suavissimus S. Lee)中UDP-糖基转移酶基因的克隆、表达及序列分析. 工业微生物, 47(4): 53-62.

9.     Meng, H., Ma, Y., Mai, G.，Wang Y*，Liu C* (2017) Construction of precise support vector machine based models for predicting promoter strength, Quant Biol. 5(1):90-98

10.  Li JH, M HL, Wang Y*(2016) Synbiological systems for complex natural products biosynthesis, Synthetic and Systems Biotechnology, 1 (04):221-229.

11.  Meng L，Xiong Z，Chu J, Wang Y* (2016) Enhanced production of avermectin by deletion of type III PKSs biosynthetic cluster rpp in Streptomyces avermitilis, Lett Appl Microbiol. 63(5):384-390

12.Lv H, Li J, Wu Y, Garyali S, Wang Y* (2016) Transporter and its engineering for secondary metabolites, Appl Microbiol Biotechnol 100(14):6119-30

13.Qi X, Lin W, Ma M, Wang C, He Y, He N, Gao J, Zhou H, Xiao Y, Wang Y, Zhang P* (2016) Structural basis of rifampin inactivation by rifampin phosphotransferase. Proc Natl Acad Sci USA. 2016 Mar 21. pii: 201523614.

14.Zhao C, Gao X, Liu X, Wang Y, Yang S, Wang F, Ren Y*(2016) Enhancing Biosynthesis of a Ginsenoside Precursor by Self-Assembly of Two Key Enzymes in Pichia pastoris. J Agric Food Chem. 64(17):3380-5

15.Meng HL, Xiong ZQ，Song SJ, Wang JF, Wang Y* (2016) Construction of polyketide overproducing E. coli strains via synthetic antisense RNAs based on in silico fluxome analysis and comparative transcriptome analysis. Biotechnol J. 11(4):530-541

16.Wang JF, Li SY, Xiong ZQ, Wang Y* (2016) Pathway mining-based integration of critical enzyme parts for de novo biosynthesis of steviol glycosides sweetener in Escherichia coli. Cell Res. 26, 258-261.

17.Meng HL, Wang Y* (2015) Cis-acting regulatory elements: from random screening to quantitative design, Quantitative Biology, 3(3), 107-114.

18.Yang JY，Xiong ZQ,Song SJ, Wang JF, Wang Y*  (2015) Improving heterologous polyketide production in Escherichia coli by transporter engineering, Appl Microbiol Biotechnol 99:8691–8700.

19.Xiong ZQ, Yang YY, Liu QX, Sun CC, Jin Y*, Wang Y* (2015) Endophytes in the plant Huperzia serrata: fungal diversity and discovery of a new pentapeptide，Arch Microbiol，197(3):411-8.

20. Xiong ZQ, Liu QX, Pan ZL, Zhao N, Feng ZX, Wang Y* (2015) Diversity and bioprospecting of culturable actinomycetes from marine sediment of the Yellow Sea, China, Arch Microbiol, 197(2): 299-309,

21. Wang JF, Xiong ZQ, Li SY, Wang Y* (2015) Exploiting exogenous MEP pathway genes to improve the downstream isoprenoid pathway effects and enhance isoprenoid production in Escherichia coli, Process Biochemistry, 50(1): 24-32,

22. 汪建峰，王勇* (2015) 基于系统-合成生物学的人工细胞网络设计, 生物产业技术, 6: 36-43.

23.   宋书杰, 熊智强, 王勇* (2015) 利用合成调控RNA技术提高大肠杆菌异源合成红霉素前体6-脱氧红霉内酯B产量, 生物工程学报, 31(7): 1039-1049.

24.   汪建峰, 蒙海林, 王勇* (2015) 萜类合成生物学研究进展, 中国科学:生命科学, 45(10): 1040-1050.

25.   孟令卓, 王勇*, 储炬* (2015) 阿维链霉菌中一个双组分调控系统SAV931/932功能初探, 华东理工大学学报（自然科学版）, 41(4): 443-448.

26.   王艳萍，熊智强，杨静亚，王勇*（2015），黄海海域沉积物和庐山土壤样品中放线菌的分离、活性及生物多样性研究，工业微生物，45（3）：24-30

27. Sun Z, Meng H, Li J, Wang J, Li Q, Wang Y*, Zhang Y* (2014). Identification of Novel Knockout Targets for Improving Terpenoids Biosynthesis in Saccharomyces cerevisiae. PLoS One; 9(11):e112615.

28. Wang JF, Xiong ZQ, Yang YY, Zhao N, Wang Y* (2014) Significant expression of a Chinese scorpion peptide, BmK1, in Escherichia coli through promoter engineering and gene dosage strategy. Biotechnol Appl Biochem, 61(4), 466-473.

29. Wang JF, Meng HL, Xiong ZQ, Zhang SL, Wang Y* (2014) Identification of novel knockout and up-regulated targets for improving isoprenoid production in E. coli. Biotechnol Lett, 36 (5): 1021-1027.

30.   欧广云, 熊智强, 周圣靓, 王勇*, 李平* (2014) 阿维链霉菌来源的磷脂酰丝氨酸合成酶基因的克隆及其在毕氏酵母中的异源表达, 工业微生物, 44(1): 28-33.

31.   汪建峰, 张嗣良*, 王勇*(2014) 大肠杆菌中从头合成白藜芦醇途径的设计及优化,  中国生物工程杂志, 34(2): 71-77.

32.   汪建峰, 熊智强, 张嗣良*, 王勇*(2014) 基于2-C-甲基-D-赤藓糖醇-4磷酸途径的产紫槐二烯大肠杆菌构建及其补糖策略优化, 生物工程学报, 30(1): 64-75.

33.   张明娥, 熊智强, 刘巧霞, 杨迎迎, 王勇*(2014) 一株真菌所产环缩酚酸肽类化合物的分离和鉴定, 华东理工大学学报（自然科学版）, 40(1): 58-64.

34.   潘钊龙, 熊智强, 刘巧霞, 郝玉有, 王勇*(2014) 海洋放线菌SMA-1代谢物多样性研究, 工业微生物, 44(4): 59-65.

35. Meng HL, Wang JF, Xiong ZQ, Xu F, Zhao GP, Wang Y* (2013) Quantitative design of regulatory elements based on high-precision strength prediction using artificial neural network. PLOS ONE, 8: e60288.

36. Wang JF, Xiong ZQ, Li SY, Wang Y* (2013) Enhancing isoprenoid production through systematically assembling and modulating efflux pumps in Escherichia coli. Appl Microbiol Biotechnol, 97: 8057-8067.

37. Xiong ZQ, Yang YY, Zhao N, Wang Y* (2013) Diversity of endophytic fungi and screening of fungal paclitaxel producer from Anglojap yew, Taxus x media. BMC Microbiol, 13: 71.

38. Xiong ZQ, Wang JF, Hao YY, Wang Y* (2013) Recent Advances in Marine Microbial Natural Products Discovery and Development. Marine Drugs, 11(3): 700-717.

39.    杨迎迎, 熊智强, 王勇*(2013) 3种药用植物内生真菌的多样性分析及产生物碱菌株的筛选, 中国抗生素杂志, 48(12): 901-908.

40.   汪建峰, 蒙海林, 熊智强, 王勇*(2013) 复杂天然产物合成人工生物系统的设计与构建, 生物工程学报, 29(8): 1146-1160.

41.   汪建峰, 蒙海林, 熊智强, 王勇*(2013) 基于合成生物学的微生物制造研究进展, 生物产业技术, 04: 12-23.

42. Xiong ZQ, Wang Y* (2012) Draft Genome Sequence of the Marine Streptomyces sp. Strain AA1529, Isolated from the Yellow Sea. J Bacteriol, 194(19): 5474-5475.

43. Xiong ZQ, Wang Y* (2012) Draft Genome Sequence of Marine-Derived Streptomyces sp. Strain AA0539, Isolated from the Yellow Sea, China. J Bacteriol, 194(23): 6622-6623.

44. Wang JF，Xiong ZQ , Meng HL, Wang YG , Wang Y* (2012) Synthetic biology triggers new era of antibiotics development, Subcell Biochem, 64: 95-114.

45.   谭亿, 林灵, 周红霞, 王以光, 郝卫清, 杨兆勇*, 王勇* (2012) 一种发酵提取物中含卤有机化合物的早期鉴别方法, 中国抗生素杂志, 37(10): 741-744.

46.   陈士林*, 朱孝轩, 李春芳, 王勇, 姚辉, 孙超, 宋经元 (2012) 中药基因组学与合成生物学, 药学学报, 47(8): 1070-1078.

47. Huan HV*, Wang Y, Zhang S.  (2012) Down-regulation of Pm4CL1 gene expression in E. coli model by antisense mRNA. Biochem Genet. 50(7-8):529-37.

48. Van Huan*, H., Van Giang, H., Van Thanh, N., Zhang, S., & Wang Y. (2012) Identification and functional analysis of the Pm4CL1 gene in transgenic tobacco plant as the basis for regulating lignin biosynthesis in forest trees. Molecular breeding, 29(1), 173-180.

49. Chen FF, Lin L, Wang L, Tan Y, Zhou HX, Wang YG, Wang Y*, He WQ* (2011) Distribution of dTDP-glucose-4,6-dehydratase gene and diversity of potential glycosylated natural products in marine sediment-derived bacteria. Appl Microbiol Biotechnol. 90(4): 1347-1359.

50. Meng HL, Wang Y*, Zhang SL, Wang XN (2011) In silico analysis and experimental improvement of the terpenoid precursor supply pathways for taxadiene heterologous biosynthesis in Escherichia coli. Biotechnol Bioprocess Eng, 16 (2): 205-215.

51. Meng HL, Lu ZG, Wang Y*, Zhang SL, Wang XN (2011) In silico analysis of erythromycin precursor 6-deoxyerythronolide B by heterologous biosynthesis in Escherichia coli. Biotechnol Bioprocess Eng, 16(3): 445-456.

52. 陈菲菲, 王勇*, 王以光, 郝卫清*(2011) 海洋微生物来源的天然产物开发研究进展,  应用与环境生物学报, 17(2): 287-294.

53. 林灵, 谭亿, 陈菲菲, 周红霞, 王以光, 郝卫清*, 王勇* (2011) 大连渤海老虎滩海域沉积物可培养放线菌的多样性, 微生物学报, 51(2): 262-269.

54. 陈菲菲, 林灵, 王以光, 周红霞, 陶佩珍, 郝卫清*, 王勇* (2011) 海洋细菌中活性化合物的筛选策略, 中国抗生素杂志, 36(5): 321-330.

55. 蒙海林, 汪建峰, 王勇*, 张嗣良, 王小宁 (2011) CYP725A4 分子序列分析与结构建模,华南理工大学学报（自然科学版）, 39(5): 154-159.

56. 卢志国, 汪建峰, 蒙海林, 熊智强, 王勇* (2011) 合成生物学与天然产物开发, 生命科学, 23(9): 900-911.

57. Van Huan*, H., Van Giang, H., Wang, Y., & Zhang, S. (2011). Overexpression and the enzymatic properties of the recombinant 4-coumarate: coenzyme A ligase, a key enzyme in lignin biosynthesis pathway. Journal of the Indian Academy of Wood Science, 8(1):  21-25.

58. Zhang HR, Wang Y, Wu JQ, Skalina K, Pfeifer BA* (2010) Complete biosynthesis of erythromycin A and designed analogs using E. coli.as a heterologous host. Chem Biol, 17(11): 1232-1240.

59. Ajikumar PK, Xiao WH, Tyo KEJ, Wang Y, Simeon F, Leonard E, Mucha O, Phon TH, Pfeifer BA*, Stephanopoulos G* (2010) Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli. Science, 330(6000): 70-74.

60. Wang Y, Zhuang YP*, Wang P, Chu J, Zhang SL (2009) Regulation of meilingmycin in Streptomyces nanchangensis: effect of ammonium ion. Korean J Chem Eng 27(3): 910-914.

61. Wang Y, Chu J, Zhuang YP, Wang YH, Xia JY, Zhang SL* (2009) Industrial bioprocess control and optimization in the context of systems biotechnology. Biotechnol Adv, 27(6): 989-995.

62. Hu H, Qian JQ*, Chu J*, Wang Y, Zhuang YP, Zhang SL (2009) DNA shuffling of methionine adenosyltransferase gene leads to improved S-adenosyl-L-methionine production in Pichia pastoris. J Biotechnol, 141(3-4): 97-103.

63. Zhang HR, Wang Y, Boghigian BA, Pfeifer BA* (2009) Probing the heterologous metabolism supporting 6-deoxyerythronolide B biosynthesis in E. coli. Microbial Biotechnol, 2(3): 390-394.

64. 郝玉有, 王勇* (2009) 微型生物反应器与高通量菌种筛选, 生物产业技术, 4: 53-59.

65. 于慧敏*, 王勇(2009) 全局转录机器工程-工业生物技术新方法, 生物产业技术, 4: 40-46.

66. Wang Y, Pfeifer BA* (2008) 6-Deoxyerythronolide B production through chromosomal localization of the deoxyerythronolide B synthase genes in E. coli. Metab Eng, 10(1): 33-38.

67. Wang Y, Wang YG*, Zhang SL (2008) High frequency transformation of the industrial erythromycin-producing bacterium Saccharopolyspora erythraea. Biotechnol Lett, 30(2): 357-361.

68. Parsa S¹, Wang Y¹(co-first author), Fuller J, Langer R, Pfeifer BA* (2008) A Comparison between Polymeric Microsphere and Bacterial Vectors for Macrophage P388D1 Gene Deliver, Pharm Res. 25 (5): 1202-1208.

69. Zhang HR¹, Wang Y¹(co-first author), Pfeifer BA* (2008) Bacterial hosts for natural product production. Mol Pharm, 5 (2): 212-225.

70. Ren YH, Wang Y, Zhang YF, Wei DZ* (2008) Overcoming multidrug resistance in a human carcinoma cells by an antisense oligodeoxynucleotide-doxorubicin conjugate in vitro and in vivo. Mol Pharm, 5(4): 579-587.

71. Parsa S¹, Wang Y¹(co-first author), Rines K, Pfeifer BA* (2008) A high-throughput comparison of recombinant gene expression parameters for E. coli.mediated gene transfer to P388D1 macrophage cells. J Biotechnol, 137(1-4): 59-64.

72. Wang Y, Boghigian BA, Pfeifer BB* (2007) Improving heterologous polyketide production in Escherichia coli by over-expression of an S-adenosylmethionine synthetase gene. Appl Microbiol Biotechnol, 77(2): 367-373.

73. Wang Y, Wang YG*，Chu J, Zhuang YP, Zhang LX*, Zhang SL* (2007) Improved production of erythromycin A by expression of a heterologous gene encoding S-adenosylmethionine synthetase. Appl Microbiol Biotechnol, 75(4): 837-842.

1.      王勇，王国栋，刘海利，拟南芥三烯甲素及其制备，申请号：201710178601.5

2.      王勇，孙雨伟，陈卓，李建华，新型 UDP-糖基转移酶及其应用，申请号：201710758391.7

3.      王勇，郝玉有，一种阵列式高通量分离培养微生物装置，中国专利，ZL 201210548502.9

4.      王勇，郝玉有，用于发酵的棉籽蛋白及其制备方法和应用，中国专利，CN 103215310 B

5.      王勇，汪建峰，熊智强，异源合成广霍香醇的方法和微生物,中国专利，申请号：2014103093474.4

6.      王勇, 熊智强, 李诗渊, 汪建峰. 高效合成萜类化合物的重组大肠杆菌底盘细胞及其制法和应用, 中国专利, 申请号: 201310501221.2

7.      王勇, 熊智强, 宋书杰, 刘巧霞，汪建峰. 一种提高大肠杆菌异源合成聚酮类化合物的方法和用途, 中国专利, 申请号: 201410338738.9.

8.      王勇, 熊智强, 李诗渊, 汪建峰. 利用微生物生产甜菊糖苷类化合物的方法, 申请号：201310460493.2；PCT专利, 国际申请号: PCT/CN2013/084618.

9.      王勇, 熊智强, 汪建峰, 李诗渊, 刘巧霞. 一种新的贝壳烯酸-13α-羟化酶、其编码基因及其应用, 申请号：201410105875.8；PCT专利, 申请号:PCT/CN2015/074344

10.   Blaine Pfeifer, Yong Wang, Genetically modified E. Coli Strains Overexpressing Genes Involved in Non-mevalonate 1-deoxy-D- xylulose-5-phosphate Synthetic Pathway and Uses Thereof’, US Patent Filed 4/2009, Application # 12/420,482.

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已指导学生

吕华军  博士研究生  071005-微生物学  

现指导学生

鄂恒超  博士研究生  071005-微生物学  

夏裕会  硕士研究生  071005-微生物学  

陈卓  硕士研究生  071005-微生物学  

刘志凤  博士研究生  071005-微生物学  

邵洁  博士研究生  071005-微生物学