彭广敦  男  博导  中国科学院广州生物医药与健康研究院
电子邮件: peng_guangdun@gibh.ac.cn
通信地址: 广东省广州市开源大道190号A103


研究领域

17世纪,起始于显微镜的发明以及对微生物的观察,“细胞学说”被提出,“所有生命体皆由细胞组成”,“细胞是生命的基本单元”以及“所有细胞都由此前的细胞变化而来”等核心概念,成为现代生物学的重要基石。然而,细胞的类型如何定义、细胞的过去现在和未来如何精确解码—这些细胞的“谱系信息”在漫长的科学研究长河中,一直是科学中的重要和基本问题。捕获细胞谱系的多维信息,尤其是探究其在时间、空间维度的精确动态变化,成了我们探索生命奥秘、解析发育程式、治愈疾病、延缓衰老的基础;对细胞谱系的精准操控,又成了基于细胞治疗、器官重建的未来再生医学应用之不可或缺的前提。本实验室围绕上述问题,主要有以下研究方向:

 

研究方向一:高通量自动化单细胞测序技术解析体内干细胞的功能分群与异质性,重绘干细胞的发育起源与命运特化机制

 

结合单细胞多组学测序、单细胞谱系示踪以及类器官等手段重点关注胚胎与组织多能干细胞的动态发育分化过程与细胞谱系决定的调控机制。

 

研究方向二:原位空间转录组技术解析空间功能特异的干细胞转录组特征,构建哺乳动物多能干细胞高分辨率的时空动态分子谱系

 

发展和利用高通量、高分辨率的空间多组学技术研究组织功能实现的细胞基础,揭示原位、动态、多维度的细胞命运决定与作用网络。

 

研究方向三:新型多能干细胞用于体外器官重建与功能修复

基于细胞谱系的新发现,实现谱系再造、损伤修复与器官的体外重建。


招生信息

欢迎有志于干细胞、发育再生以及生物信息学等相关领域研究的同学报考研究生。

招生专业
071009-细胞生物学
071008-发育生物学
0710J3-生物信息学
招生方向
干细胞的分子谱系
空间多组学
生物信息学

教育背景

2002-09--2009-07   中国科学院上海生物化学与细胞生物学研究所   博士学位

工作经历

   

教授课程

医学发育生物学
发育生物学中的新技术

专利与奖励

   
奖励信息
(1) 中国生命科学十大进展, 部委级, 2019
(2) 2018年度创新人才之星, 市地级, 2018

出版信息

   
发表论文
[1] Xia, Qing, Cui, Guizhong, Fan, Ye, Wang, Xiuqin, Hu, Gongcheng, Wang, Lisha, Luo, Xi, Yang, Lele, Cai, Qingqing, Xu, Kaibiao, Guo, Wenjing, Gao, Minghui, Li, Yingying, Wu, Ji, Li, Wei, Chen, Jiayu, Qi, Huayu, Peng, Guangdun, Yao, Hongjie. RNA helicase DDX5 acts as a critical regulator for survival of neonatal mouse gonocytes. CELL PROLIFERATION[J]. 2021, 54(5): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088469/.
[2] Zhang, Ke, Yu, Fang, Zhu, Jian, Han, Sue, Chen, Jiehui, Wu, Xuanyuan, Chen, Yingying, Shen, Tingyu, Liao, Jiaoyang, Guo, Wenke, Yang, Xianfa, Wang, Ran, Qian, Yun, Yang, Jiaxin, Cheng, Leping, Zhao, Yun, Hui, ChiChung, Li, Jinsong, Peng, Guangdun, He, Shuijin, Jing, Naihe, Tang, Ke. Imbalance of Excitatory/Inhibitory Neuron Differentiation in Neurodevelopmental Disorders with an NR2F1 Point Mutation. CELL REPORTS[J]. 2020, 31(3): http://dx.doi.org/10.1016/j.celrep.2020.03.085.
[3] 崔桂忠, 彭广敦, 景乃禾. 高精度时空转录组揭示小鼠早期胚胎三胚层细胞谱系发生过程. 中国细胞生物学学报[J]. 2020, 42(1): 1-8, http://lib.cqvip.com/Qikan/Article/Detail?id=7101519066.
[4] Shim, Woo Jun, Sinniah, Enakshi, Xu, Jun, Vitrinel, Burcu, Alexanian, Michael, Andreoletti, Gaia, Shen, Sophie, Sun, Yuliangzi, Balderson, Brad, Boix, Carles, Peng, Guangdun, Jing, Naihe, Wang, Yuliang, Kellis, Manolis, Tam, Patrick P L, Smith, Aaron, Piper, Michael, Christiaen, Lionel, Quan Nguyen, Boden, Mikael, Palpant, Nathan J. Conserved Epigenetic Regulatory Logic Infers Genes Governing Cell Identity. CELL SYSTEMS[J]. 2020, 11(6): 625-+, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781436/.
[5] He, Di Demi, Tang, Xinyu Thomas, Dong, Wenjie, Cui, Guizhong, Peng, Guangdun, Yin, Xiujuan, Chen, Yujie, Jing, Naihe, Zhou, Bo O. C-KIT Expression Distinguishes Fetal from Postnatal Skeletal Progenitors. STEM CELL REPORTS[J]. 2020, 14(4): 614-630, http://dx.doi.org/10.1016/j.stemcr.2020.03.001.
[6] Peng, Guangdun, Cui, Guizhong, Ke, Jincan, Jing, Naihe, Chakravarti, A, Green, ED. Using Single-Cell and Spatial Transcriptomes to Understand Stem Cell Lineage Specification During Early Embryo Development. ANNUAL REVIEW OF GENOMICS AND HUMAN GENETICS, VOL 21, 2020null. 2020, 21: 163-181, https://www.webofscience.com/wos/woscc/full-record/WOS:000613915300009.
[7] 无, 景乃禾, 彭广敦, 韩敬东, 索生宝, 崔桂忠, 禹方. 小鼠早期胚胎细胞发育的四维时空图谱. 中国科技成果. 2020, 64-66, http://lib.cqvip.com/Qikan/Article/Detail?id=7101982845.
[8] Wenqian Song, Ran Wang, Weimin Jiang, Qi Yin, Guangdun Peng, Ruikai Yang, Qing Cissy Yu, Jianfeng Chen, Jingsong Li, Tom H Cheung, Naihe Jing, Yi Arial Zeng. Hormones induce the formation of luminal-derived basal cells in the mammary gland. 细胞研究:英文版[J]. 2019, 29(3): 206-220, http://lib.cqvip.com/Qikan/Article/Detail?id=7001759775.
[9] Yu, Wei, Ma, Xueyan, Xu, Jinjin, Heumueller, Andreas Wilhelm, Fei, Zhaoliang, Feng, Xue, Wang, Xiaodong, Liu, Kuo, Li, Jinhui, Cui, Guizhong, Peng, Guangdun, Ji, Hongbin, Li, Jinsong, Jing, Naihe, Song, Hai, Lin, Zhiqiang, Zhao, Yun, Wang, Zuoyun, Zhou, Bin, Zhang, Lei. VGLL4 plays a critical role in heart valve development and homeostasis. PLOS GENETICS[J]. 2019, 15(2): https://doaj.org/article/7010330f90d545dcbf61dde5eb272596.
[10] Song, Wenqian, Wang, Ran, Jiang, Weimin, Yin, Qi, Peng, Guangdun, Yang, Ruikai, Yu, Qing Cissy, Chen, Jianfeng, Li, Jingsong, Cheung, Tom H, Jing, Naihe, Zeng, Yi Arial. Hormones induce the formation of luminal-derived basal cells in the mammary gland (vol 29, pg 206, 2019). CELL RESEARCHnull. 2019, 29(4): 338-338, https://www.webofscience.com/wos/woscc/full-record/WOS:000462861400012.
[11] Cheng, Shangli, Pei, Yu, He, Liqun, Peng, Guangdun, Reinius, Bjorn, Tam, Patrick P L, Jing, Naihe, Deng, Qiaolin. Single-Cell RNA-Seq Reveals Cellular Heterogeneity of Pluripotency Transition and X Chromosome Dynamics during Early Mouse Development. CELL REPORTS[J]. 2019, 26(10): 2593-+, http://dx.doi.org/10.1016/j.celrep.2019.02.031.
[12] Cui, Guizhong, Jing, Naihe, Peng, Guangdun. Comments on 'Molecular architecture of lineage allocation and tissue organization in early mouse embryo'. JOURNAL OF MOLECULAR CELL BIOLOGYnull. 2019, 11(11): 1024-1025, http://lib.cqvip.com/Qikan/Article/Detail?id=7100725240.
[13] Liu, Qiaozhen, Liu, Kuo, Cui, Guizhong, Huang, Xiuzhen, Yao, Shun, Guo, Wenke, Qin, Zhen, Li, Yan, Yang, Rui, Pu, Wenjuan, Zhang, Libo, He, Lingjuan, Zhao, Huan, Yu, Wei, Tang, Muxue, Tian, Xueying, Cai, Dongqing, Nie, Yu, Hu, Shengshou, Ren, Tao, Qiao, Zengyong, Huang, Hefeng, Zeng, Yi Arial, Jing, Naihe, Peng, Guangdun, Ji, Hongbin, Zhou, Bin. Lung regeneration by multipotent stem cells residing at the bronchioalveolar-duct junction. NATURE GENETICS[J]. 2019, 51(4): 728-+, [14] Yifei Zhou, Xueqing Wang, Mengjiao Song, Zhidong He, Guizhong Cui, Guangdun Peng, Christoph Dieterich, Adam Antebi, Naihe Jing, Yidong Shen. A secreted microRNA disrupts autophagy in distinct tissues of Caenorhabditis elegans upon ageing. NATURE COMMUNICATIONS[J]. 2019, 10(1): http://dx.doi.org/10.1038/s41467-019-12821-2.
[15] Osteil, Pierre, Studdert, Josh B, Goh, Hwee Ngee, Wilkie, Emilie E, Fan, Xiaochen, Khoo, PohLynn, Peng, Guangdun, Salehin, Nazmus, Knowles, Hilary, Han, JingDong J, Jing, Naihe, Fossat, Nicolas, Tam, Patrick P L. Dynamics of Wnt activity on the acquisition of ectoderm potency in epiblast stem cells. DEVELOPMENT[J]. 2019, 146(7): [16] Yang, Xianfa, Hu, Boqiang, Liao, Jiaoyang, Qiao, Yunbo, Chen, Yingying, Qian, Yun, Feng, Su, Yu, Fang, Dong, Ji, Hou, Yu, Xu, He, Wang, Ran, Peng, Guangdun, Li, Jinsong, Tang, Fuchou, Jing, Naihe. Distinct enhancer signatures in the mouse gastrula delineate progressive cell fate continuum during embryo development. CELL RESEARCH[J]. 2019, 29(11): 911-926, http://lib.cqvip.com/Qikan/Article/Detail?id=7100567575.
[17] Song, Wenqian, Wang, Ran, Jiang, Weimin, Yin, Qi, Peng, Guangdun, Yang, Ruikai, Yu, Qing Cissy, Chen, Jianfeng, Li, Jingsong, Cheung, Tom H, Jing, Naihe, Zeng, Yi Arial. Hormones induce the formation of luminal-derived basal cells in the mammary gland. CELL RESEARCH[J]. 2019, 29(3): 206-220, http://lib.cqvip.com/Qikan/Article/Detail?id=7001759775.
[18] Peng, Guangdun, Suo, Shengbao, Cui, Guizhong, Yu, Fang, Wang, Ran, Chen, Jun, Chen, Shirui, Liu, Zhiwen, Chen, Guoyu, Qian, Yun, Tam, Patrick P L, Han, JingDong J, Jing, Naihe. Molecular architecture of lineage allocation and tissue organization in early mouse embryo. NATURE[J]. 2019, 572(7770): 528-+, [19] Han Xue, Luo Sai, Peng Guangdun, Lu J Yuyang, Cui Guizhong, Liu Lichao, Yan Pixi, Yin Yafei, Liu Wei, Wang Ran, Zhang Jiejie, Ai Shanshan, Chang Zai, Na Jie, He Ibin, Jing Naihe, Shen Xiaohua. Mouse knockout models reveal largely dispensable but context-dependent functions of IncRNAs during development. 分子细胞生物学报:英文版[J]. 2018, 10(2): 175-178, http://lib.cqvip.com/Qikan/Article/Detail?id=675347937.
[20] Yang, Xianfa, Hu, Boqiang, Hou, Yu, Qiao, Yunbo, Wang, Ran, Chen, Yingying, Qian, Yun, Feng, Su, Chen, Jun, Liu, Chang, Peng, Guangdun, Tang, Fuchou, Jing, Naihe. Silencing of developmental genes by H3K27me3 and DNA methylation reflects the discrepant plasticity of embryonic and extraembryonic lineages. CELL RESEARCHnull. 2018, 28(5): 593-596, http://lib.cqvip.com/Qikan/Article/Detail?id=675657274.
[21] Christodoulou, Neophytos, Kyprianou, Christos, Weberling, Antonia, Wang, Ran, Cui, Guizhong, Peng, Guangdum, Jing, Naihe, ZernickaGoetz, Magdalena. Sequential formation and resolution of multiple rosettes drive embryo remodelling after implantation. NATURE CELL BIOLOGY[J]. 2018, 20(11): 1278-+, https://www.webofscience.com/wos/woscc/full-record/WOS:000448320700010.
[22] Liu, Chang, Wang, Ran, He, Zhisong, Osteil, Pierre, Wilkie, Emilie, Yang, Xianfa, Chen, Jun, Cui, Guizhong, Guo, Wenke, Chen, Yingying, Peng, Guangdun, Tam, Patrick P L, Jing, Naihe. Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells. STEM CELL REPORTS[J]. 2018, 11(1): 43-57, http://dx.doi.org/10.1016/j.stemcr.2018.05.019.
[23] Friedman, Clayton E, Quan Nguyen, Lukowski, Samuel W, Helfer, Abbigail, Chiu, Han Sheng, Miklas, Jason, Levy, Shiri, Suo, Shengbao, Han, JingDong Jackie, Osteil, Pierre, Peng, Guangdun, Jing, Naihe, Baillie, Greg J, Senabouth, Anne, Christ, Angelika N, Bruxner, Timothy J, Murry, Charles E, Wong, Emily S, Ding, Jun, Wang, Yuliang, Hudson, James, RuoholaBaker, Hannele, BarJoseph, Ziv, Tam, Patrick P L, Powell, Joseph E, Palpant, Nathan J. Single-Cell Transcriptomic Analysis of Cardiac Differentiation from Human PSCs Reveals HOPX-Dependent Cardiomyocyte Maturation. CELL STEM CELL[J]. 2018, 23(4): 586-+, http://202.127.25.143/handle/331003/3468.
[24] Liu Chang, Peng Guangdun, Jing Naihe. TGF-β signaling pathway in early mouse development and embryonic stem cells. 生物化学与生物物理学报:英文版[J]. 2018, 50(1): 68-73, http://lib.cqvip.com/Qikan/Article/Detail?id=674784452.
[25] Liu, Chang, Peng, Guangdun, Jing, Naihe. TGF-beta signaling pathway in early mouse development and embryonic stem cells. ACTA BIOCHIMICA ET BIOPHYSICA SINICAnull. 2018, 50(1): 68-73, https://www.webofscience.com/wos/woscc/full-record/WOS:000423304200007.
[26] Chang Liu, Guangdun Peng, Naihe Jing. TGF-β signaling pathway in early mouse development and embryonic stem cells. 生物化学与生物物理学报:英文版. 2018, 50(1): 68-73, http://lib.cqvip.com/Qikan/Article/Detail?id=674784452.
[27] Cui, Guizhong, Suo, Shengbao, Wang, Ran, Qian, Yun, Han, JingDong J, Peng, Guangdun, Tam, Patrick P L, Jing, Naihe. Mouse gastrulation: Attributes of transcription factor regulatory network for epiblast patterning. DEVELOPMENT GROWTH & DIFFERENTIATION[J]. 2018, 60(8): 463-472, http://202.127.25.143/handle/331003/3440.
[28] Han, Xue, Luo, Sai, Peng, Guangdun, Lu, J Yuyang, Cui, Guizhong, Liu, Lichao, Yan, Pixi, Yin, Yafei, Liu, Wei, Wang, Ran, Zhang, Jiejie, Ai, Shanshan, Chang, Zai, Na, Jie, He, Aibin, Jing, Naihe, Shen, Xiaohua. Mouse knockout models reveal largely dispensable but context-dependent functions of lncRNAs during development. JOURNAL OF MOLECULAR CELL BIOLOGYnull. 2018, 10(2): 175-178, http://202.127.25.143/handle/331003/3441.
[29] Guangdun Peng, JingDong J Han. Regulatory network characterization in development: challenges and opportunities. F1000Research. 2018, 7: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6144950/.
[30] Sibbritt, Tennille, Ip, Chi K, Khoo, PohLynn, Wilkie, Emilie, Jones, Vanessa, Sun, Jane Q J, Shen, Joanne X, Peng, Guangdun, Han, JingDong J, Jing, Naihe, Osteil, Pierre, Ramialison, Mirana, Tam, Patrick P L, Fossat, Nicolas. A gene regulatory network anchored by LIM homeobox 1 for embryonic head development. GENESIS[J]. 2018, 56(9): http://202.127.25.143/handle/331003/3368.
[31] 彭广敦. Lineage specification of early embryos and embryonic stem cells at the dawn of enabling technologie. Natl Sci Rev. 2017, [32] Peng, Guangdun, Jing, Naihe. The genome-wide molecular regulation of mouse gastrulation embryo. SCIENCE CHINA-LIFE SCIENCESnull. 2017, 60(4): 363-369, https://www.webofscience.com/wos/woscc/full-record/WOS:000401068300004.
[33] Guangdun Peng, Patrick P. L. Tam, Naihe Jing. Lineage specification of early embryos and embryonic stem cells at the dawn of enabling technologies. 国家科学评论:英文版. 2017, 4(4): 533-542, http://lib.cqvip.com/Qikan/Article/Detail?id=673781631.
[34] Chen, Jun, Suo, Shengbao, Tam, Patrick P L, Han, JingDong J, Peng, Guangdun, Jing, Naihe. Spatial transcriptomic analysis of cryosectioned tissue samples with Geo-seq. NATURE PROTOCOLS[J]. 2017, 12(3): 566-580, [35] Wang, Lingbo, Li, MinYin, Qu, Chao, Miao, WanYing, Yin, Qi, Liao, Jiaoyang, Cao, HuaTeng, Huang, Min, Wang, Kai, Zuo, Erwei, Peng, Guangdun, Zhang, ShuXin, Chen, Guodong, Li, Qing, Tang, Ke, Yu, Qian, Li, Zhoujie, Wong, Catherine C L, Xu, Guoliang, Jing, Naihe, Yu, Xiang, Li, Jinsong. CRISPR-Cas9-mediated genome editing in one blastomere of two-cell embryos reveals a novel Tet3 function in regulating neocortical development. CELL RESEARCH[J]. 2017, 27(6): 815-829, https://www.webofscience.com/wos/woscc/full-record/WOS:000402545200011.
[36] Peng, Guangdun, Tam, Patrick P L, Jing, Naihe. Lineage specification of early embryos and embryonic stem cells at the dawn of enabling technologies. NATIONAL SCIENCE REVIEWnull. 2017, 4(4): 533-542, http://lib.cqvip.com/Qikan/Article/Detail?id=673781631.
[37] Yuanyuan Li, Ran Wang, Nan Qiao, Guangdun Peng, Ke Zhang, Ke Tang, JingDong J Han, Naihe Jing. Transcriptome analysis reveals determinant stages controlling human embryonic stem cell commitment to neuronal cells. The Journal of Biological Chemistry. 2017, 292(48): 19590-19604, http://dx.doi.org/10.1074/jbc.M117.796383.
[38] Zamir, Lyad, Singh, Reena, Nathan, Elisha, Patrick, Ralph, Yifa, Oren, YahalomRonen, Yfat, Arrafi, Alaa A, Schultheiss, Thomas M, Suo, Shengbao, Han, JingDong Jackie, Peng, Guangdun, Jing, Naihe, Wang, Yuliang, Palpant, Nathan, Tam, Patrick P L, Harveyz, Richard P, Tzahor, Eldad. Nkx2.5 marks angioblasts that contribute to hemogenic endothelium of the endocardium and dorsal aorta. ELIFE[J]. 2017, 6: https://doaj.org/article/91a9da3c78ca4d5aac16920103726488.
[39] Lu Song, Jun Chen, Guangdun Peng, Ke Tang, Naihe Jing. Dynamic Heterogeneity of Brachyury in Mouse Epiblast Stem Cells Mediates Distinct Response to Extrinsic Bone Morphogenetic Protein (BMP) Signaling *. The Journal of Biological Chemistry. 2016, 291(29): 15212-15225, http://dx.doi.org/10.1074/jbc.M115.705418.
[40] Peng, Guangdun, Suo, Shengbao, Chen, Jun, Chen, Weiyang, Liu, Chang, Yu, Fang, Wang, Ran, Chen, Shirui, Sun, Na, Cui, Guizhong, Song, Lu, Tam, Patrick P L, Han, JingDong J, Jing, Naihe. Spatial Transcriptome for the Molecular Annotation of Lineage Fates and Cell Identity in Mid-gastrula Mouse Embryo. DEVELOPMENTAL CELL[J]. 2016, 36(6): 681-697, http://dx.doi.org/10.1016/j.devcel.2016.02.020.
[41] Li, Lingyu, Song, Lu, Liu, Chang, Chen, Jun, Peng, Guangdun, Wang, Ran, Liu, Pingyu, Tang, Ke, Rossant, Janet, Jing, Naihe. Ectodermal progenitors derived from epiblast stem cells by inhibition of Nodal signaling. JOURNAL OF MOLECULAR CELL BIOLOGY[J]. 2015, 7(5): 455-465, https://www.webofscience.com/wos/woscc/full-record/WOS:000363201800007.
[42] Liu, Pingyu, Dou, Xiaoyang, Liu, Chang, Wang, Lingbo, Xing, Can, Peng, Guangdun, Chen, Jun, Yu, Fang, Qiao, Yunbo, Song, Lu, .... Histone deacetylation promotes mouse neural induction by restricting Nodal-dependent mesendoderm fate. NATURE COMMUNICATIONS[J]. 2015, 6: http://www.irgrid.ac.cn/handle/1471x/968125.
[43] Tang, Ke, Peng, Guangdun, Qiao, Yunbo, Song, Lu, Jing, Naihe. Intrinsic regulations in neural fate commitment. DEVELOPMENT GROWTH & DIFFERENTIATIONnull. 2015, 57(2): 109-120, https://www.webofscience.com/wos/woscc/full-record/WOS:000350479100003.
[44] Zhu, Qingqing, Song, Lu, Peng, Guangdun, Sun, Na, Chen, Jun, Zhang, Ting, Sheng, Nengyin, Tang, Wei, Qian, Cheng, Qiao, Yunbo, Tang, Ke, Han, JingDong Jackie, Li, Jinsong, Jing, Naihe. The transcription factor Pou3f1 promotes neural fate commitment via activation of neural lineage genes and inhibition of external signaling pathways. ELIFE[J]. 2014, 3: https://doaj.org/article/41b05fe34676495fa3fa0b7a33ff13ac.

科研活动

   
科研项目
( 1 ) 中胚层的早期模式建成研究, 主持, 国家级, 2019-01--2022-12
( 2 ) 间充质干细胞亚群的功能鉴定、分离制备与疗效评估, 主持, 国家级, 2018-01--2022-12
( 3 ) 肌肉再生、制造中的神经化探索, 主持, 部委级, 2017-01--2021-12
( 4 ) 内胚层起源及内胚层细胞体内分化的调控机制, 参与, 国家级, 2017-01--2021-12
( 5 ) 广东省自然科学基金杰出青年基金, 主持, 省级, 2019-10--2023-09
参与会议
(1)LINEAGE SEGREGATION OF POST- IMPLANTATION MOUSE EMBRYO REVEALED BY SPATIAL AND SINGLE CELL TRANSCRIPTOME   国际干细胞学会2017年年会   2017-06-22
(2)Lineage segregation of post-implantation mouse embryo revealed by spatial and single cell transcriptome   日本发育生物学学会50年纪念年会   2017-05-12