Prof. Mingliang Ye

Professor of Chemistry

Key Lab of Separation Sciences for Analytical Chemistry
National Chromatographic R&A Center
Dalian Institute of Chemical Physics
Chinese Academy of Sciences, Dalian 116023, China




Research Areas

1)      New approaches for analysis of protein post-translational modifications 

2)      New proteomics approaches to elucidate the enzyme-substrate relationship

3)      Mass spectrometry-based approach for identification and quantification of proteins


Education and Professional Experiences:


Ph.D student in Analytical Chemistry,

Supervisor Prof. Hanfa Zou

Dalian Institute of Chemical Physics, CAS,



Postdoctoral fellow

Advisor, Prof. Norman Dovichi

University of Washington, Seattle, USA



Postdoctoral fellow

Advisor, Prof. Ruedi Aebersold

Institute for System Biology, Seattle, USA



     Associate Professor

     Dalian Institute of Chemical Physics, CAS




     Dalian Institute of Chemical Physics, CAS


1) Jiawei Mao, Xin You, Hongqiang Qin, Chengye Wang, Liming Wang, Mingliang Ye*. A New Searching Strategy for the Identification of O‑Linked Glycopeptides. Analytical Chemistry, 2019, 91, 3852−3859

2) Qi Wang, Zhen Liu, Keyun Wang, Yan Wang, Mingliang Ye*. A new chromatographic approach to analyze methylproteome with enhanced lysine methylation identification performance. Analytica Chimica Acta, 2019, 1068, 111-119

3) Yating Yao, Yan Wang, Shujuan Wang, Xiaoyan Liu, Zhen Liu, Yanan Li, Zheng Fang, Jiawei Mao, Yong Zheng, Mingliang Ye*. One-Step SH2 Superbinder-Based Approach for Sensitive Analysis of Tyrosine Phosphoproteome. Journal of Proteome Research, 2019, 18, 1870−1879

4) Yanan Li, Yan Wang, Jiawei Mao, Yating Yao, Keyun Wang, Qinglong Qiao, Zheng Fang, Mingliang Ye*. Sensitive profiling of cell surface proteome by using an optimized biotinylation method. Journal of Proteomics, 2019, 196, 33-41

5) Xin You, Yating Yao, Jiawei Mao, Hongqiang Qin, Xinmiao Liang, Liming Wang, Mingliang Ye*. Chemoenzymatic Approach for the Proteomics Analysis of Mucin- Type Core‑1 O‑Glycosylation in Human Serum. Analytical Chemistry, 2018, 90, 12714−12722

6) Xin You, Hongqiang Qin, Jiawei Mao, Yu Tian, Mingming Dong, Zhimou Guo, Xinmiao Liang, Liming Wang, Yan Jin, Mingliang Ye*. Highly Efficient Identification of O-GalNAc Glycosylation by an Acid-Assisted Glycoform Simplification Approach. Proteomics, 2018, 18, 1800042

7) Mingming Dong, Yating Yao, Yan Wang, Yan Jin, Mingliang Ye*. The divide and conquer strategies for deep phosphoproteomics analysis. Trends in Analytical Chemistry, 2018, 105, 282-291

8) Jiawen Lyu, Yan Wang, Jiawei Mao, Yating Yao, Shujuan Wang, Yong Zheng, Mingliang Ye*. Pseudotargeted MS Method for the Sensitive Analysis of Protein Phosphorylation in Protein Complexes. Analytical Chemistry, 2018, 90,6214-6221

9) Yating Yao, Yangyang Bian, Mingming Dong, Yan Wang, Jiawen Lv, Lianfang Chen, Hongwei Wang, Jiawei Mao, Jing Dong, Mingliang Ye*. SH2 Superbinder Modified Monolithic Capillary Column for the Sensitive Analysis of Protein Tyrosine Phosphorylation. Journal of Proteome Research, 2018, 17, 243-251

10) Xin You, Hongqiang Qin, Mingliang Ye*. Recent advances in methods for the analysis of protein o-glycosylation at proteome level. Journal of  Separation Sciences, 2018, 41, 248-261

11)  Zhenzhen Deng, Jiawei Mao, Yan Wang, Hanfa Zou, Mingliang Ye*. Enzyme kinetics for complex system enables accurate determination of specificity constants of numerous substrates in a mixture by proteomics platform. Mol. Cell. Proteomics, 2017, 16(1), 135-145

12)  Yanan Li, Yan Wang, Mingming Dong, Hanfa Zou, Mingliang Ye*. Sensitive Approaches for the Assay of the Global Protein Tyrosine Phosphorylation in Complex Samples Using a Mutated SH2 Domain. Anal. Chem., 2017, 89 (4), 2304–2311

13) Zhenzhen Deng, Mingming Dong, Yan Wang, Jing Dong, Shawn S-C Li, Hanfa Zou, Mingliang Ye*. Biphasic Affinity Chromatographic Approach for Deep Tyrosine Phosphoproteome Analysis. Anal. Chem. 2017, 89(4), 2405−2410

14)  Xuefang Dong, Hongqiang Qin, Jiawei Mao, Dongping Yu, Xiuling Li, Aijin Shen, Jingyu Yan, Long Yu, Zhimou Guo, Mingliang Ye*, Hanfa Zou, Xinmiao Liang*. In-depth Analysis of Glycoprotein Sialylation in Serum Using a Dual-Functional Material with Superior Hydrophilicity and Switchable Surface Charge.  Anal. Chem., 2017(7), 89, 3966–3972

15)  Haiyang Zhang, Junjie Ou*, Yating Yao, Hongwei Wang, Zhongshan Liu, Yinmao Wei*, and Mingliang Ye*. Facile Preparation of Titanium(IV)-Immobilized Hierarchically Porous Hybrid Monoliths. Anal. Chem., 2017, 89(8), 4655–4662

16) Yuting Cong, Lianghai Hu*, Zhang Zhang, Yin Gao, Mingming Dong, Hongqiang Qin, Mingliang Ye*, Jingkai Gu, Hanfa Zou. Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study. Talanta, 2017, 165, 664–670

17)  Hongqiang Qin, Kai Cheng, Jun Zhu, Jiawei Mao, Fangjun Wang, Mingming Dong, Rui Chen, Zhimou Guo, Xinmiao Liang, Mingliang Ye*, Hanfa Zou. Proteomics Analysis of O-GalNAc Glycosylation in Human Serum by an Integrated Strategy, Anal. Chem., 2017, 89(3):1469-1476

18) Yangyang Bian, Lei Li, Mingming Dong, Xuguang Liu, Tomonori Kaneko, Kai Cheng, Huadong Liu, Courtney Voss, Xuan Cao, Yan Wang, David Litchfield, Mingliang Ye*, Shawn SC Li*, Hanfa Zou*. Ultra-deep tyrosine phosphoproteomics enabled by a phosphotyrosine superbinder. Nat. Chem. Biol., 2016, 12, 959-968

19) Keyun Wang, Mingming Dong, Jiawei Mao, Yan Wang, Yan Jin, Mingliang Ye*, Hanfa Zou. Antibody-Free Approach for the Global Analysis of Protein Methylation.Anal. Chem., 2016, 2016, 88(23), 11319-11327

20) Ye, Mingliang*; Pan, Yanbo; Cheng, Kai; Zou, Hanfa*. Protein Digestion Priority is Independent of Protein Abundances. Nat. Methods, 2014, 11: 220-222.

21) Pan, Yanbo; Ye, Mingliang*; Zhao, Liang; Cheng, Kai; Dong, Mingming; Song, Chunxia; Qin,Hongqiang; Wang, Fangjun; Zou, Hanfa*. N-Terminal Labeling of Peptides by Trypsin-Catalyzed Ligation for Quantitative Proteomics. Angew. Chem. Int. Ed., 2013, 52(35): 9205-9209.

Research Interests

1Mass spectrometry based protein identification approaches  

2​New methods for the analysis of protein post translational modifications

      3New approaches to screen the enzyme substrates, inhibitors

      4Bioinformatics methods to facilitate the interpretation of MS data

5Label free and isotope labeling based quantitative proteomics

6High efficient separation schemes for separation of peptides and proteins



张曼玉  硕士研究生  070302-分析化学  

王春丽  博士研究生  070302-分析化学  

程凯  博士研究生  070302-分析化学  

边阳阳  博士研究生  070302-分析化学  

潘彦博  博士研究生  070302-分析化学  

郑昊  硕士研究生  070302-分析化学  

王科云  博士研究生  070302-分析化学  

张章  博士研究生  070302-分析化学  

刘芳洁  博士研究生  070302-分析化学  

邓真真  博士研究生  070302-分析化学  

王红卫  博士研究生  070302-分析化学  

王  博士研究生  070302-分析化学  

由昕  博士研究生  070302-分析化学  

姚亚婷  博士研究生  070302-分析化学  

张娜  博士研究生  070302-分析化学  

刘震  博士研究生  070302-分析化学  

李亚楠  博士研究生  070302-分析化学  

吕佳纹  博士研究生  070302-分析化学  

陈尧  博士研究生  070302-分析化学  

王麒  博士研究生  070302-分析化学  

方正  博士研究生  070302-分析化学  

阮成飞  博士研究生  070302-分析化学  


李柯佳  博士研究生  070302-分析化学  

刘璐瑶  博士研究生  070302-分析化学  

叶玉莹  硕士研究生  070302-分析化学  

苗恩铭  博士研究生  070302-分析化学  

朱赫  博士研究生  070302-分析化学  

王钟毓  博士研究生  070302-分析化学  

刘蕾  硕士研究生  070302-分析化学  

于婷  博士研究生  070302-分析化学  

马燕妮  博士研究生  070302-分析化学  

薛连基  硕士研究生  070302-分析化学  

岳旭阳  博士研究生  070302-分析化学  

朱海洋  博士研究生  070302-分析化学  

刘莉  博士研究生  070302-分析化学