微流控技术

070302-分析化学
071010-生物化学与分子生物学
083100-生物医学工程

微流控
质谱分析
基因测序

2007-09--2012-07   北京大学工学院   博士学位
2003-09--2009-07   吉林大学   学士学位

北京大学 先进材料与力学 博士

北京大学 先进材料与力学 博士

   

2018-10~2019-09,美国加州大学伯克利分校, 访问学者
2017-08~现在, 中国科学院深圳先进技术研究院, 副研究员
2016-10~2017-08,美国加州大学戴维斯分校, 专职副研究员
2014-10~2016-10,美国圣母大学, 博士后
2012-09~2014-07,北京大学生命科学学院, 博士后

   

（1） 第二十四届高交会优秀产品奖, 一等奖, 市地级, 2022

[1] 王冠博, 朱重亲, 门涌帆, 李雯, 黄岩谊. 一种用于蛋白样品溶液置换或蛋白分离的装置和方法. CN: CN117339642A, 2024-01-05.
[2] 王冠博, 门涌帆, 李雯, 朱重亲, 黄岩谊. 用于蛋白样品溶液置换或蛋白分离的装置和方法. CN: CN117339643A, 2024-01-05.
[3] 吴永光, 门涌帆, 郑焱, 刘梦羽, 廖九零, 马佳艳, 谢龙旭. 一种用于细胞分选仪的激光装置. CN: CN219657449U, 2023-09-08.
[4] 门涌帆, 孔维俊. 聚合物微球制备装置和制备方法. CN: CN115178198B, 2023-11-03.
[5] 门涌帆, 孔维俊. 聚合物微球制备装置和制备方法. CN: CN115178198A, 2022-10-14.
[6] 门涌帆, 孔维俊. 微流控电喷雾装置. CN: CN218516678U, 2023-02-24.
[7] 门涌帆, 李雯, 吴碧珠. 一种微通道阵列板的嵌套结构的制备方法和液滴产生装置. CN: CN112571718A, 2021-03-30.
[8] 门涌帆, 李雯, 吴碧珠. 一种嵌套结构及制造其的模具和液滴产生装置. CN: CN212247006U, 2020-12-29.
[9] 陈艳, 门涌帆, 潘挺睿, 舒伟良, 吴碧珠. 一种微流液滴生成装置. CN: CN110339881A, 2019-10-18.
[10] 门涌帆, 陈艳, 冯鸿涛, 敖婷婷. 基于微流控电喷雾的单细胞全基因组扩增系统和方法. CN: CN110317728A, 2019-10-11.
[11] 门涌帆, 潘挺睿, 敖婷婷, 李致昊, 吴碧珠, 陈艳. 基于微流控液滴打印系统的数字PCR检测方法及应用. CN: CN110295109A, 2019-10-01.
[12] 门涌帆, 李雯, 吴碧珠. 一种嵌套结构的制备方法. CN: CN109491203A, 2019-03-19.
[13] CHANG, Hsueh-Chia, GO, David B., SLOUKA, Zdenek, SENAPATI, Satyajyoti, MEN, Yongfan, PAN, Zehao. AC ELECTROSPRAYED DROPLETS FOR DIGITAL AND EMULSION PCR. 世界知识产权组织: WO2017209906(A1), 2017.12.07.
[14] CHANG, Hsueh-Chia, GO, David B., SLOUKA, Zdenek, SENAPATI, Satyajyoti, MEN, Yongfan, PAN, Zehao. AC ELECTROSPRAYED DROPLETS FOR DIGITAL AND EMULSION PCR. US: US20190352698(A1), 2019-11-21.

   

[1] Kong, Weijun, Feng, Hongtao, Qian, Xiang, Chen, Yizhao, Deng, Mengying, Zhang, Pengfei, Li, Wen, Bu, Wenting, Xu, Wenchao, Jin, Wei, Huang, Yuqing, Chu, Jun, Wu, Shangtao, Chen, Yan, Men, Yongfan. Facile and scalable generation of fluorescent microspheres using a microfluidic electrojetting device. SENSORS AND ACTUATORS B-CHEMICAL[J]. 2023, 第 15 作者  通讯作者  378: http://dx.doi.org/10.1016/j.snb.2022.133106.
[2] Yang, Zhiyi, Li, Wenbo, Huang, Hao, Ren, Songlei, Men, Yongfan, Li, Fang, Yu, Xuefeng, Luo, Qian. Detection of serum phospholipids by microchannel-integrated black phosphorus-assisted laser desorption/ionization mass spectrometry. TALANTA[J]. 2022, 第 5 作者237: http://dx.doi.org/10.1016/j.talanta.2021.122978.
[3] Zhang, Xiannian, Qian, Zhenwei, Jiang, Mengcheng, Li, Wentao, Huang, Yanyi, Men, Yongfan. Design and High-Resolution Analysis of an Efficient Periodic Split-and-Recombination Microfluidic Mixer. MICROMACHINES[J]. 2022, 第 6 作者  通讯作者  13(10): http://dx.doi.org/10.3390/mi13101720.
[4] Tan, Wen, Pan, Tingrui, Wang, Shengpeng, Li, Peng, Men, Yongfan, Tan, Rui, Zhong, Zhangfeng, Wang, Yitao. Immunometabolism modulation, a new trick of edible and medicinal plants in cancer treatment. FOOD CHEMISTRY. 2022, 第 5 作者376: http://dx.doi.org/10.1016/j.foodchem.2021.131860.
[5] 门涌帆. Microfluidic Platform for Time-Resolved Characterization of Protein Higher-Order Structures and Dynamics Using Top-Down Mass Spectrometry. Analytical Chemistry[J]. 2022, 第 1 作者  通讯作者  
[6] Liu, Li, Song, Wen, Huang, Shijia, Jiang, Kai, Moriwaki, Yoshitaka, Wang, Yichuan, Men, Yongfan, Zhang, Dan, Wen, Xing, Han, Zhifu, Chai, Jijie, Guo, Hongwei. Extracellular pH sensing by plant cell-surface peptide-receptor complexes. CELL[J]. 2022, 第 7 作者185(18): 3341-+, http://dx.doi.org/10.1016/j.cell.2022.07.012.
[7] Huang, Xuelin, Jiao, Zhe, Guo, Zongning, Yang, Jialing, Alam, Parvej, Liu, Yong, Men, Yongfan, Zhang, Pengfei, Feng, Haitao, Yao, Shuhuai, Tang, Ben Zhong. Development of Reaction-Based AIE Handy Pen for Visual Detection of Toxic Vapors. ACS MATERIALS LETTERS[J]. 2021, 第 7 作者3(2): 249-254, http://dx.doi.org/10.1021/acsmaterialslett.0c00516.
[8] Bu, Wenting, Li, Wen, Li, Jiannan, Ao, Tingting, Li, Zhihao, Wu, Bizhu, Wu, Shangtao, Kong, Weijun, Pan, Tingrui, Ding, Yi, Tan, Wen, Li, Baoqing, Chen, Yan, Men, Yongfan. A low-cost, programmable, and multi-functional droplet printing system for low copy number SARS-CoV-2 digital PCR determination. SENSORS AND ACTUATORS B-CHEMICAL[J]. 2021, 第 14 作者  通讯作者  348: http://apps.webofknowledge.com/CitedFullRecord.do?product=UA&colName=WOS&SID=5CCFccWmJJRAuMzNPjj&search_mode=CitedFullRecord&isickref=WOS:000700931400004.
[9] Pan, Yang, Ma, Tuo, Meng, Qi, Mao, Yuxin, Chu, Kaiqin, Men, Yongfan, Pan, Tingrui, Li, Baoqing, Chu, Jiaru. Droplet digital PCR enabled by microfluidic impact printing for absolute gene quantification. TALANTA[J]. 2020, 第 6 作者211: http://dx.doi.org/10.1016/j.talanta.2019.120680.
[10] Xiangzhong Sun, Yuping Qiu, Yang Peng, Juewei Ning, Guangjie Song, Yanzhu Yang, Mengyu Deng, Yongfan Men, Xingzhong Zhao, Yichuan Wang, Hongwei Guo, Yanqing Tian. Close Temporal Relationship between Oscillating Cytosolic K + and Growth in Root Hairs of Arabidopsis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES[J]. 2020, 第 8 作者21(17): https://doaj.org/article/176e45b74cc74009a7894da28a3000e0.
[11] Sun, Xiangzhong, Qiu, Yuping, Peng, Yang, Ning, Juewei, Song, Guangjie, Yang, Yanzhu, Deng, Mengyu, Men, Yongfan, Zhao, Xingzhong, Wang, Yichuan, Guo, Hongwei, Tian, Yanqing. Close Temporal Relationship between Oscillating Cytosolic K(+)and Growth in Root Hairs ofArabidopsis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES[J]. 2020, 第 8 作者21(17): https://www.webofscience.com/wos/woscc/full-record/WOS:000570332200001.
[12] Tan, Wen, Zhong, Zhangfeng, Carney, Randy P, Men, Yongfan, Li, Jiannan, Pan, Tingrui, Wang, Yitao. Deciphering the metabolic role of AMPK in cancer multi-drug resistance. SEMINARS IN CANCER BIOLOGY[J]. 2019, 第 4 作者56: 56-71, http://dx.doi.org/10.1016/j.semcancer.2018.09.005.
[13] Li, Jiannan, Tan, Wen, Xiao, Wenwu, Carney, Randy P, Men, Yongfan, Li, Yuanpei, Quon, Gerald, Ajena, Yousif, Lam, Kit S, Pan, Tingrui. A Plug-and-Play, Drug-on-Pillar Platform for Combination Drug Screening Implemented by Microfluidic Adaptive Printing. ANALYTICAL CHEMISTRY[J]. 2018, 第 5 作者90(23): 13969-13977, http://ir.siat.ac.cn:8080/handle/172644/14445.
[14] Pan, Zehao, Men, Yongfan, Senapati, Satyajyoti, Chang, HsuehChia. Immersed AC electrospray (iACE) for monodispersed aqueous droplet generation. BIOMICROFLUIDICS[J]. 2018, 第 2 作者12(4): http://ir.siat.ac.cn:8080/handle/172644/14441.
[15] Fan, Jinzhen, Men, Yongfan, Tseng, Kuo Hao, Ding, Yi, Ding, Yunfeng, Villarreal, Fernando, Tan, Cheemeng, Li, Baoqing, Pan, Tingrui. Dotette: Programmable, high-precision, plug-and-play droplet pipetting. BIOMICROFLUIDICS[J]. 2018, 第 2 作者12(3): http://ir.siat.ac.cn:8080/handle/172644/14440.
[16] Meiling Zhang, Tao Sun, Chongshu Jian, Lei Lei, Peidong Han, Quanlong Lv, Ran Yang, Xiaohai Zhou, Jiejia Xu, Yingchun Hu, Yongfan Men, Yanyi Huang, Chuanmao Zhang, Xiaojun Zhu, Xianhua Wang, Heping Cheng, JingWei Xiong. Remodeling of Mitochondrial Flashes in Muscular Development and Dystrophy in Zebrafish. PLOS ONE[J]. 2015, 第 11 作者10(7): https://doaj.org/article/6633d6c6f4ba4aa79ee6ae7e48536409.
[17] Xian, Bo, Shen, Jie, Chen, Weiyang, Sun, Na, Qiao, Nan, Jiang, Dongqing, Yu, Tao, Men, Yongfan, Han, Zhijun, Pang, Yuhong, Kaeberlein, Matt, Huang, Yanyi, Han, JingDong J. WormFarm: a quantitative control and measurement device toward automated Caenorhabditis elegans aging analysis. AGING CELL[J]. 2013, 第 8 作者12(3): 398-409, http://dx.doi.org/10.1111/acel.12063.
[18] Fei, Peng, Chen, Zitian, Men, Yongfan, Li, Ang, Shen, Yiran, Huang, Yanyi. A compact optofluidic cytometer with integrated liquid-core/PDMS-cladding waveguides. LAB ON A CHIP[J]. 2012, 第 3 作者12(19): 3700-3706, http://dx.doi.org/10.1039/c2lc40329c.
[19] 门涌帆. A high-throughput imaging system to quantitatively analyze the growth dynamics of plant seedlingsw. INTEGRATIVEBIOLOGY. 2012, 第 1 作者
[20] 门涌帆. Digital polymerase chain reaction in an array of femtoliter PDMS microreactors.. Analytical Chemistry. 2012, 第 1 作者
[21] Fei, Peng, He, Zi, Zheng, Chunhong, Chen, Tao, Men, Yongfan, Huang, Yanyi. Discretely tunable optofluidic compound microlenses. LAB ON A CHIP[J]. 2011, 第 5 作者11(17): 2835-2841, http://dx.doi.org/10.1039/c1lc20425d.
[22] Zong, Zhaocun, Yu, Hai, Nui, Lianping, Zhang, Mingzhe, Wang, Chen, Li, Wei, Men, Yongfan, Yao, Binbin, Zou, Guangtian. Potential-induced copper periodic micro-/nanostructures by electrodeposition on silicon substrate. NANOTECHNOLOGY[J]. 2008, 第 7 作者19(31): https://www.webofscience.com/wos/woscc/full-record/WOS:000257166800006.

   

（ 1 ） 新型高灵敏度柔性仿生触觉传感技术研发及产业化, 参与, 地方任务, 2017-07--2022-06
（ 2 ） 数字智能微流控芯片联合实验室, 参与, 境内委托项目, 2018-07--2019-07
（ 3 ） 基于电喷雾原理的超微液滴发生微机电系统研究, 负责人, 地方任务, 2019-04--2021-03
（ 4 ） 基于微流控技术的肿瘤微环境细胞间Wnt信号互作的研究, 负责人, 地方任务, 2019-04--2022-03
（ 5 ） 适用于单细胞全基因组扩增的微流控交流电喷雾方法研究, 负责人, 国家任务, 2020-01--2022-12
（ 6 ） 基于微流控技术的细胞分选仪器及配套芯片技术开发项目, 负责人, 境内委托项目, 2020-04--2022-04
（ 7 ） 应用于临床全血样品多组学分离鉴定与定量分析的一体化微流控系统, 参与, 地方任务, 2019-01--2021-12
（ 8 ） 全自动微滴式数字PCR系统关键技术研发, 负责人, 地方任务, 2021-04--2024-04
（ 9 ） 基于微流控-质谱联用技术研究kindlin在肺癌Wnt信号通路中的作用机制, 负责人, 其他国际合作项目, 2020-11--2023-10
（ 10 ） 微流道阵列芯片制备, 负责人, 境内委托项目, 2020-01--2022-12
（ 11 ） DNA勘验核心装备自主研制及可控应用关键技术研究, 参与, 国家任务, 2021-11--2024-10

（1）Droplet Digital PCR Reaction Enabled by Microfluidic Printing   第二届微流控技术 应用创新论坛   2018-12-08
（2）Droplet Digital PCR Reaction Enabled by Microfluidic Printing   2018-12-02
（3）Droplet Digital PCR Reaction Enabled by Microfluidic Printing,   2018-10-20
（4）Droplet Digital PCR Reaction Enabled by Microfluidic Printing   2018中国生物医学 工程学会天津青年论坛（BME2018Young）   2018-08-03
（5）CloudPette: Cloud-Based Ultrahigh-Precision Microfluidic Pipetting   2017-10-14
（6）An Ingegrated and Automated Multiplexed Biochip Platform for Point-of-Care Applications: Robust Complexity   2015-08-18