Biochemistry & Molecular biology

Structural biology

Genetics

2018-present    Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences/Principal Investigator

2016-2018        UCB Pharma/Senior Scientist

2013-2016        The Clare Hall Laboratories, the Francis Crick Institute/Post-doctoral Research Fellow

2012-2013        The Institute of Cancer Research/Post-doctoral Scientist

1. Dong, S. #, Li, H. #, Wang, M. #, Rasheed, N. #, Zou, B., Gao, X., Guan, J., Li, W., Zhang, J., Wang, C., Zhou, N., Shi, X., Li, M., Zhou, M., Huang, J., Li, H., Zhang, Y., Wong, K.H., Zhang, X., Chao, W.C.H.*, and He, J*. Structural basis of nucleosome deacetylation and DNA linker tightening by Rpd3S histone deacetylase complex. Cell Res 33, 790-801. (2023)

2. Zhang, X. #, Li, Z. #, Zhang, Y. #, Liu, Y. #, Wang, J., Liu, B., Chen, Q., Wang, Q., Fu, L., Wang, P., Zhong, X., Jin, L., Yan, Q., Chen, L., He, J.*, Zhao, J. *, and Xiong, X*. Disulfide stabilization reveals conserved dynamic features between SARS-CoV-1 and SARS-CoV-2 spikes. Life Sci Alliance 6. (2023)

3. Yu, H. #, Liu, B. #, Zhang, Y. #, Gao, X. #, Wang, Q. #, Xiang, H. #, Peng, X. #, Xie, C. #, Wang, Y. #, Hu, P., Shi, J., Shi, Q., Zheng, P., Feng, C., Tang, G., Liu, X., Guo, L., Lin, X., Li, J., Liu, C., Huang, Y., Yang, N., Chen, Q., Li, Z., Su, M., Yan, Q., Pei, R., Chen, X., Liu, L., Hu, F., Liang, D., Ke, B., Ke, C. *, Li, F. *, He, J. *, Wang, M.*, Chen, L.*, Xiong, X.*, and Tang, X*. Somatically hypermutated antibodies isolated from SARS-CoV-2 Delta infected patients cross-neutralize heterologous variants. Nat Commun 14, 1058. (2023)

4. Tang, L. #, Dong, S. #, Rasheed, N. #, Wu, H.W. #, Zhou, N. #, Li, H., Wang, M., Zheng, J.*, He, J.*, and Chao, W.C.H*. Vibrio parahaemolyticus prey targeting requires autoproteolysis-triggered dimerization of the type VI secretion system effector RhsP. Cell Rep 41, 111732. (2022)

5. He, P. #, Liu, B. #, Gao, X. #, Yan, Q. #, Pei, R. #, Sun, J., Chen, Q., Hou, R., Li, Z., Zhang, Y., Zhao, J., Sun, H., Feng, B., Wang, Q., Yi, H., Hu, P., Li, P., Zhang, Y., Chen, Z., Niu, X., Zhong, X., Jin, L., Liu, X., Qu, K., Ciazynska, K.A., Carter, A.P., Briggs, J.A.G., Chen, J., Liu, J., Chen, X.*, He, J.*, Chen, L.*, and Xiong, X*. SARS-CoV-2 Delta and Omicron variants evade population antibody response by mutations in a single spike epitope. Nat Microbiol 7, 1635-1649. (2022)

6. Chen, M. #, He, Y. #, Liu, D., Tian, L., Xu, P., Liu, X., Pan, Y., Dong, S., He, J. *, and Zhang, Y*. Structure Insights Into Photosystem I Octamer From Cyanobacteria. Front Microbiol 13, 876122. (2022)

7. Chen, M. #, Liu, X., He, Y., Li, N., He, J.*, and Zhang, Y*. Diversity Among Cyanobacterial Photosystem I Oligomers. Front Microbiol 12, 781826. (2021)

8. Frigola, J. #, He, J. #, Kinkelin, K., Pye, V.E., Renault, L., Douglas, M.E., Remus, D., Cherepanov, P. *, Costa, A. *, and Diffley, J.F.X*. Cdt1 stabilizes an open MCM ring for helicase loading. Nat Commun 8, 15720. (2017)

9. He, J. #, Chao, W.C. #, Zhang, Z., Yang, J., Cronin, N., and Barford, D*. Insights into degron recognition by APC/C coactivators from the structure of an Acm1-Cdh1 complex. Mol Cell 50, 649-660. (2013)

10. He, J. #, Kulkarni, K., da Fonseca, P.C., Krutauz, D., Glickman, M.H., Barford, D.*, and Morris, E.P*. The structure of the 26S proteasome subunit Rpn2 reveals its PC repeat domain as a closed toroid of two concentric alpha-helical rings. Structure 20, 513-521. (2012)

11. Zhang, C. #, Li, L., He, J., Chen, C., and Su, D*. Nonstructural protein 7 and 8 complexes of SARS-CoV-2. Protein Sci 30, 873-881. (2021)

12. Zhang, C. #, Chen, Y., Li, L., Yang, Y., He, J., Chen, C. *, and Su, D*. Structural basis for the multimerization of nonstructural protein nsp9 from SARS-CoV-2. Mol Biomed 1, 5. (2020)

13. da Fonseca, P.C. #, He, J., and Morris, E.P*. Molecular model of the human 26S proteasome. Mol Cell 46, 54-66. (2012)

14. Wang, X. et al. A potent human monoclonal antibody with pan-neutralizing activities directly dislocates S trimer of SARS-CoV-2 through binding both up and down forms of RBD. Signal Transduct Target Ther 7, 114. (2022)

15. Qu, K. #, Chen, Q., Ciazynska, K.A., Liu, B., Zhang, X., Wang, J., He, Y., Guan, J., He, J., Liu, T., Zhang, X., Carter, A.P., Xiong, X.*, and Briggs, J.A.G*. Engineered disulfide reveals structural dynamics of locked SARS-CoV-2 spike. PLoS Pathog 18, e1010583. (2022)

I am interested in studying mechanisms of macromolecular machines that play fundamental roles in cell fate determination by multi-disciplinary approaches. Most proteins form a network of interactions with other proteins and many are components of large complexes in vivo. We focus on those which play fundamental roles within the cell and aim to establish fundamental principles underlying the assembly of multi-protein complexes, define their structures, gain insight into their mechanisms using cryo-electron microscopy and x-ray crystallography. In the past, we have been successfully applied this hybrid approach to various macromolecular machines including proteasome, E3 ubiquitin ligase and DNA replication complex. We are now implementing these technologies to investigating fundamental mechanism of cell fate determination. We are also developing cutting-edge methodologies that can faithfully image the native cellular environment at high-resolution, aiming to reveal a complete structural description of the cell's native molecular landscape at certain stage.  

已指导学生

邹彬倩  硕士研究生  085238-生物工程  

现指导学生

张家乐  博士研究生  071010-生物化学与分子生物学  

董淑琦  博士研究生  071010-生物化学与分子生物学  

赵河豫  博士研究生  071010-生物化学与分子生物学  

苏小凤  硕士研究生  086000-生物与医药  

罗窍鸿  硕士研究生  086000-生物与医药  

2019              Huangpu Talent Program, Guangzhou City

2018              High-Level Talent Program, Chinese Academy of Sciences

2013              Chairman’s Prize for Best PhD, the Institute of Cancer Research

2012              Government Award for outstanding students abroad