Fluorescent proteins, Genetically encoded fluorescent indicators, optical imaging, GPCR signaling pathway

Start and end years	Institution	Position
2004/9-2009/9	Huazhong University of Science and Technology	Ph.D.
2001/9-2004/7	Huazhong University of Science and Technology	Master
1997/9-2001/7	Huazhong University of Science and Technology	Bachelor

   

Start and end years	Institution	Position
2016/12-now	SIAT, CAS	Professor
2015/3-2016/12	SIAT, CAS	Associate Professor
2010/8-2015/3	Stanford University	Postdoctoral Fellow
2009/10-2010/8	UMASS, Amherst	Postdoctoral Fellow

1.     Han Y, Yang J, Li Y, Chen Y, Ren H, Ding R, Qian W, Ren K, Xie B, Deng M, Xiao Y, Chu J, Zou P. Bright and sensitive red voltage indicators for imaging action potentials in brain slices and pancreatic islets. Science Advances, 2023.

2.     Chai Y, Qi K, Wu Y, Li D, Tan G, Guo Y, Chu J, Mu Y, Shen C, Wen Q. All-optical interrogation of brain-wide activity in freely swimming larval zebrafish. iScience, 2023.

3.     Hu Y, Yang F, Liu C, Wang N, Wang X, Zhang R, Gao L, Xu M, Wang J, Liu Z, Huang S, Liu W, Hu Y, Liu F, Guo Y, Wang L, Yuan J, Zhang Z*, Chu J*. UFObow: a single-wavelength excitable Brainbow for simultaneous multicolor imaging of mammalian cells ex vivo and in vivo. Communications Biology, Accepted.

4.     Wang L, Wu C, Peng W, Zhou Z, Zeng J, Li X, Yang Y, Yu S, Zou Y, Huang M, Liu C, Chen Y, Ti P, Liu W, Gao Y, Zheng W, Gao S, Lu Z, Ren P, Ng H, He J, Chen S, Xu M, Li Y, Chu J. A high-performance genetically enclded fluorescent indicator for in vivo cAMP imaging. Nature Communications, 2022.

5.     Liu F, Hu H, Deng M, Xiang Z, Guo Y, Guan X, Li D, Hu Q, Lei W^*, Peng H^*, Chu J^*. A Bright Monomeric Near-Infrared Fluorescent Protein with an Excitation Peak at 633 nm for Labeling Cellular Protein and Reporting Protein-Protein Interaction. ACS Sensors, 2022.

6.     Ning L, Geng Y, Lovett-Barron M, Niu X, Deng M, Wang L, Ataie N, Sens A, Ng HL, Chen S, Deisseroth K, Lin MZ^*, Chu J^*. A Bright, Nontoxic, and Non-aggregating red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons. Front Cell Dev Biol., 2022. 

7.     Liu W, Liu C, Ren PG, Chu J^*, Wang L^*. An Improved Genetically Encoded Fluorescent cAMP Indicator for Sensitive cAMP Imaging and Fast Drug Screening. Front Pharmacol. 2022.

8.     Bajar B, Guan X, Lam A, Lin M, Yasuda R, Laviv T^*, Chu J^*. FRET imaging of Rho GTPase activity with red fluorescent protein-based FRET pairs. Methods in Molecular Biology, 2022.

9.     Gao R, Liu F, Liu W, Zeng S, Chen J, Gao R, Wang L, Fang C, Song L, Sedgwick A, Sessller J^*, Chu J^*, Yan F^*, Liu C^*. Background-suppressed tumor-targeted photoacoustic imaging using bacterial carriers. PNAS, 2022.

10.  Deng M, Yuan J, Yang H, Wu X, Wei X, Du Y, Wong G, Tao Y, Liu G, Jin Z*, Chu J*. A Genetically Encoded Bioluminescent System for Fast and Highly Sensitive Detection of Antibodies with a Bright Green Fluorescent Protein. ACS Nano, 2021.

11.  Chen X, Liu T, Qin X, Nguyen QQ, Lee SK, Lee C, Ren Y, Chu J, Zhu G, Yoon TY, Park CY, Park H. Simultaneous Real-Time Three-Dimensional Localization and FRET Measurement of Two Distinct Particles. Nano Letters., 2021.

12.  Teng B, Huang C, Cheng C, Udduttula A, Yu X, Liu C, Li J, Yao Z, Long J, Miao L, Zou C, Chu J, Zhang J*, Ren P*. Newly Identified Peptide Hormone Inhibits Intestinal Fat Absorption and Improves NAFLD Through Its Receptor GPRC6A. Journal of Hepatology, 2020.

13.  Tian F, Lin TC, Wang L, Chen S, Chen X, Yiu PM, Tsui OKC, Chu J, Kiang CH, Park H

Mechanical Responses of Breast Cancer Cells to Substrates of Varying Stiffness Revealed by Single-Cell Measurements. J Phys Chem Lett,. 2020.

14.  Laviv T, Scholl B, Parra-Bueno P, Foote B, Zhang C, Yan L, Hayano Y, Chu J, Yasuda R. In Vivo Imaging of the Coupling between Neuronal and CREB Activity in the Mouse Brain. Neuron, 2020, 105(5): 799-812.e5.

15.  Qin X, Liu L, Lee SK, Alsina A, Liu T, Wu C, Park H, Yu C, Kim H, Chu J, Triller A, Tang BZ, Hyeon C, Park CY, Park H. Increased Confinement and Polydispersity of STIM1 and Orai1 after Ca²⁺ Store Depletion. Biophys Journal. 2020.

16.  Xu Y, Deng M, Zhang S, Yang J, Peng L, Chu J*, Zou P*. Imaging Neuronal Activity with Fast and Sensitive Red-Shifted Electrochromic FRET Indicators. ACS Chemical Neuroscience, 2019, 10, 4768-4775.

17.  Liu W, Deng M, Yang C, Liu F, Guan X, Du Y, Wang L*, Chu J*. Genetically encoded single circularly permuted fluorescent protein-based intensity indicators. Journal of Physics D: Applied Physics, 2019, 53 113001.

18.  Chang Z, Liu F, Wang L, Deng M, Zhou C, Sun Q*, Chu J*. Near-infrared dyes, nanomaterials and proteins. Chinese Chemical Letters, 2019, 30(10): 1856-1882.

19.  Bajar BT, Emily ES, Zhang S, Lin MZ, Chu J*. A guide to fluorescent protein FRET pairs. Sensors (Basel), 2016, 16(9).

20.  Liu C, Gong X, Lin R, Chen J, Wang Z, Liang S*, Chu J*. Advances in imaging techniques and genetically encoded probes for photoacoustic imaging. Theranostics, 2016, 6(13): 2414-2430 .

21.  Chu J, Dana H, Douglas K, Yang H, Chen X, Lin MZ. A bright cyan-excitable oragne fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo. Nature Biotechnology, 2016, 34(7): 760-767.

22.  Bajar BT, Emily ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, Lin MZ*, Chu J*. Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. Scientific Reports, 2016, 6: 20889.

23.  Chu J, Haynes RD, Corbel SY, Li PP, Burg JS, Ataie NJ, Lam AJ, Gonzalez EZ, Cranfill PJ, Baird M, Davdson MW, Ng HL, Garcia CC, Contag CH, Blau HM, Lin MZ. Non-invasive intravital imaging of cellular regeneration with a bright red-excitable fluorescent protein. Nature Methods, 2014, 11(5): 572-578.

24.  Bajar BT, Lam AJ, Badiee RK, Oh Y, Chu J, Zhou XX, Kim N, Kim BB, Chung M, Yablonovitch AL, Cruz BF, Kulalert K, Tao JJ, Meyer T, Su XD, Lin MZ. Fluorescent indicators for simultaneous reporting of all four cell cycle phases. Nature Methods, 2016, 13(12): 993-996.

25.  Laviv T, Kim BB, Chu J, Lam AJ, Lin MZ, Yasuda R. Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted fluorophores. Nature Methods, 2016, 13(12): 989-992.

26.  Welf ES, Driscoll MK, Dean KM, Schäfer C, Chu J, Davidson MW, Lin MZ, Danuser G, Fiolka R. Quantitative Multiscale Cell Imaging in Controlled 3D Microenvironments. Dev Cell. 2016, 36(4): 462-475.

27.  Nicholls SB, Chu J, Abbruzzese G, Tremblay KD, Hardy J. Mechanism of a genetically-encoded dark-to-bright reporter for caspase activity. Journal of Biological Chemistry, 2011.

28.  Chu J, Xing Y, Lin MZ. Far-red and near-infraed fluorescent protein. The Fluorescent Protein Revolution. Day R and Davidson M, Eds. (CRC Press, Boca Raton, Florida, USA, 2014).

Fluorescent proteins, FP-based fluorescent indicators, optical imaging of signaling pathway