In vitro synthetic biosystems are emerging biomanufacturing platform. Compared to current microbial fermentation platform, in vitro synthetic biosystems have many advantages, such as high reaction rate, high product yield, broad reaction condition. Our group is focusing on the construction and optimization of in vitro synthetic biosystems for the production of food, energy and bulk chemicals.

2003-2009      PhD, Fudan University, Microbial Genetics, China

1999-2003      B.S. Fudan University, Biological Science, China

2016.3-Present   Professor, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China

2015.3-2016.3    Chief Scientist, Cell Free Bioinnovations. Inc, USA

2012.4-2016.4    Senior Research Associate, Virginia Tech, USA

2010.4-2012.4    Postdoctoral Associate, Virginia Tech, USA

2009.6-2010.4    Research Assistant, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China

1. Journal paper

1)           YOU C*, Huang R, Zhu ZG, Zhang Y-HP*. 2017 Protein engineering on nicotinamide-based coenzymes with its applications to synthetic biology. Synthetic and Systems Biotechnology 2: 208-218.

2)           YOU C, Shi T, Li YJ, Han PP, Zhou XG, Zhang Y-HP*. 2017. An in vitro synthetic biology platform for the industrial biomanufacturing of myo-inositol from starch. Biotechnology and Bioengineering 114: 1855-1864.

3)            YOU C*, Zhang Y-HP. 2017. Biomanufacturing by in vitro biosystems containing cascade enzymes. Process Biochemistry. 52: 106-114.

4)       YOU C, Chen HG, Myung S, Sathisuksanoh N, Ma H, Zhang XZ, Li JY, Zhang Y-HP*.2013. Enzymatic transformation of non-food biomass to starch. Proceedings of the National Academy of Sciences of the USA. 110: 7182-7189.  (Highlighted by Science magazine)

5)       YOU C, Myung S, Zhang Y-HP*. 2012. Facilitated substrate channeling in a self-assembled trifunctional enzyme complex. Angewandte Chemie International Edition.51: 8787-8790

6)       YOU C, Zhang X-Z , Sathitsuksanoh N , Lynd LR, Zhang Y-HP*. 2012. Enhanced microbial cellulose utilization of recalcitrant cellulose by an ex vivo cellulosome-microbe complex. Applied and Environmental Microbiology 78(5):1437-1444

7)       YOU C, Zhang X-Z, Zhang Y-HP*. 2012. Simple Cloning via direct transformation of PCR product (DNA multimer) to Escherichia coli and Bacillus subtilis. Applied and Environmental Microbiology 78(5):1593-1595

8)       YOU C, Zhang Y-HP*. 2012. Easy preparation of a large-size random gene mutagenesis library in Escherichia coli. Analytical Biochemistry 428:7-12

9)       YOU C, Zhang X-Z, Zhang Y-HP*. 2012. Mini-scaffoldin enhanced mini-cellulosome hydrolysis performance on low-accessibility cellulose (Avicel) more than on high-accessibility amorphous cellulose. Biochemical Engineering Journal 63:57-65

10)   YOU C, Zhang Y-HP*. 2013. Self-assembly of synthetic metabolons through synthetic scaffoldins: single-step purification, co-immobilization, and substrate channeling. ACS Synthetic Biology 2:102-110, (cover page).

11)   YOU C, Zhang Y-HP*. 2014. Annexation of a high-activity rate-limiting enzyme in a synthetic three-enzyme complex greatly decreases the degree of substrate channeling. ACS Synthetic Biology, 3(6): 380-386

12)   YOU C, Huang Q, Xue HP, Xu Y, Lu H *. 2010. Potential hydrophobic interaction between two cysteines in interior hydrophobic region improves thermostability of a family 11 xylanase from Neocallimastix patriciarum. Biotechnology and Bioengineering 105(5):861-70

13)       Zhong C, YOU C, Wei P, Zhang Y-HP*. 2017. Thermal cycling cascade biocatalysis of myo-inositol synthesis from sucrose. ACS Catalysis. 7: 5992–5999.

14)   Kim JE, Huang R, Chen H, YOU C, Zhang Y-HP. 2016. Facile construction of random gene mutagenesis library for directed evolution without the use of restriction enzyme in Escherichia coli. Biotechnol J. 11:1142-1150.

15)   Zhou W, YOU C, Ma H, Ma Y, Zhang YH. 2016. One-Pot Biosynthesis of High-Concentration alpha-Glucose 1-Phosphate from Starch by Sequential Addition of Three Hyperthermophilic Enzymes. J Agric Food Chem. 64:1777-1783.

16)   Rollin JA, Martin del Campo J, Myung S, Sun F, YOU C, Bakovic A, Castro R, Chandrayan SK, Wu CH, Adams  MWW, Senger RS, Zhang Y-HP, 2015. High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling. Proceedings of the National Academy of Sciences. 112 (16), 4964-4969.

17)   Zhu ZG, Tam TK, Sun FF, YOU C, Zhang Y-HP*. 2014. A high-energy-density sugar biobattery based on a synthetic enzymatic pathway. Nature Communications. 5: 3026,

18)   Martin del Campo JS, Rollin JR, Myung S, YOU C, Chandrayan S, Patiño R,  Adams MWW, Zhang Y-HP*. 2013. Dihydrogen production from xylose and water mediated by synthetic cascade enzymes. Angewandte Chemie International Edition 52:4587-4590,

19)   Myung S, YOU C, Zhang Y-HP*. 2013. Recyclable cellulose-containing magnetic nanoparticles: immobilization of cellulose-binding module-tagged proteins and synthetic metabolon featuring substrate channeling. Journal of Materials Chemistry B. 1:4419-4427.

20)   Martin del Campo JS, YOU C, Kim J-E, Patiño R, Zhang Y-HP*. 2013. Discovery and characterization of a novel ATP/polyphosphate xylulokinase from a hyperthermophilic bacterium Thermotoga maritima. Journal of Industrial Microbiology and Biotechnology 40:661-669.

21)   Myung S, Rollin J, YOU C, Sun F, Chandrayan S, Adams MWW, Zhang Y-HP. 2014. In vitro metabolic engineering of hydrogen production at theoretical yield from sucrose. Metabolic Engineering 24(0):70-77.

22)   Peng Q, YOU C, Zhang Y-HP*, 2014, One-Pot Enzymatic Conversion of Sucrose to Synthetic Amylose by using Enzyme Cascades. ACS Catalysis. 4:1311-1317.

23)   Gao SH, YOU C, Renneckar S, Bao J, Zhang Y-HP*. 2014. New insights into enzymatic hydrolysis of heterogeneous cellulose by using CBM3-containing GFP and CBM17-containing CFP. Biotechnology for Biofuels.  7(1):24

24)   Xue HP, Zhou JG, YOU C, Huang Q, Lu H *. 2012. Amino acid substitutions in the N-terminus, cord and α-helix domains improved the thermostability of a family 11 xylanase XynR8. Journal of Industrial Microbiology and Biotechnology 39:1279-1288

25)   Zhou JG, Bao L, Chang L, Liu Z, YOU C, Lu H *. 2012. Beta-xylosidase activity of a GH3 glucosidase/xylosidase from yak rumen metagenome promotes the enzymatic degradation of hemicellulosic xylans. Letters in Applied Microbiology 54:79-87

26)   Zhang Y-HP*, Myung S, YOU C, Zhu ZG, Rollin J. 2011. Toward low-cost biomanufacturing through cell-free synthetic biology: bottom-up design. Journal of Materials Chemistry 21: 18877-18886

2、Book Chapter

1)       YOU C*, Zhang Y-HP*. Ex Vivo Enzymatic Conversion of Non-food Cellulose Biomass to Starch. In: P. C. K. Lau editor. Quality Living Through Chemurgy and Green Chemistry. Berlin, Heidelberg: Springer Berlin Heidelberg; 2016. p. 129-142.

2)       YOU C, Zhang Y-HP*. 2013. Cell-free biosystems for biomanufacturing. Advances in Biochemical Engineering/Biotechnology 131:89-119.

3)       YOU C, Zhang Y-HP*. 2014. Simple cloning and DNA assembly in E. coli by prolonged overlap extension PCR. Methods of Molecular Biology 1116。

4)       Zhang Y-HP*, YOU C, Chen HG, Feng RL. 2012. Surpassing photosynthesis: high-efficiency and scalable CO2 utilization through artificial photosynthesis. ACS Symposium Series 1097: 275-292 (Recent Advances in Post-Combustion CO₂ Capture Chemistry), Oxford University Press, UK

5)       Jandt U, YOU C, Zhang Y-HP, Zeng A-P*. 2013. Compartmentation and metabolic channeling: practical and modeling aspects for multienzymatic biosynthesis. Advances in Biochemical Engineering/Biotechnology 137:41-65.

6)       Zhong C, YOU C, Wei P, Zhang Y-HP*. 2017. Simple cloning by prolonged overlap extension PCR with application to the preparation of large-size random gene mutagenesis library in Escherichia coli. Methods in Molecular Biology.1472: 49-61.

1)  Zhang Yiheng, You, Chun, Inositol preparing method CN 106148425A

2)  Zhang Yiheng, You, Chun,Zhong Chao, A inositol preparing method from sucrose, application NO. 201611035068.9

3)  Lv, Hong, You, Chun, Huang, Qiang, High temperature and strong alkali resistant xylanase improved gene, genetic engineering bacterial strain thereof and preparation method thereof, CN 101392266 B

已指导学生

孙尚尚  博士研究生  071010-生物化学与分子生物学  

现指导学生

刘美霞  博士研究生  081703-生物化工  

李强子  博士研究生  081703-生物化工  

覃艳梅  硕士研究生  081703-生物化工  

樊霖  硕士研究生  0710J5-食品安全与健康  

National 1000 Youth program award