基本信息

邱金龙,博士、研究员、中国科学院“****”入选者

电话:010-64807398
电子邮件:qiujl#im.ac.cn(请将#换成@

通讯地址:北京市朝阳区北辰西路1号院3号,
中国科学院微生物研究所
邮编:100101

1991年毕业于山东大学生物系,获学士学位。1994年于华东师范大学生物系获硕士学位。1997年 华东师范大学生物系/中科院上海植物生理所植物分子遗传学国家重点实验室联合培养博士。1998年至2009年在欧美多个著名研究机构从事研究工作。2009年中科院****引进国外杰出人才进入中科院微生物所植物基因组学国家重点实验室工作。


研究领域

研究方向:植物抗病机制及生物技术

主要研究内容:1、植物免疫的蛋白磷酸化信号网络;2、基因组编辑技术及应用

1植物应对不同外界病原微生物刺激的蛋白磷酸化信号网络的解析。植物受体识别病原微生物后以一系列蛋白磷酸化过程将信号传递到细胞核内,调控防卫基因的表达,激发防卫反应。因此,蛋白磷酸化是免疫信号在植物细胞内的主要传递方式。本实验室利用蛋白组学等高通量的研究手段系统分析单子叶作物识别病原后细胞内蛋白激酶及蛋白磷酸化变化及其动态过程,并结合蛋白相互作用等信息通过系统生物学方法构建相应的磷酸化网络,进而深入了解植物免疫信号转导的作用方式及调控机制。

2) 基因组编辑技术及应用。基因组定向编辑(Genome editing)技术是近年来发展起来的一项革命性的分子遗传学研究方法,其核心是利用人工核酸酶在基因组靶定位点引起DNA双链断裂,激活非同源末端连接(NHEJ)或同源重组(HR)DNA损伤修复途径,从而引起基因的定向突变。该技术主要利用三种人工核酸酶:锌指核酸酶(zinc-finger nucleasesZFNs),TALE核酸酶(transcription activator like effector nucleasesTALENs)和CRISPR/Cas9系统(CRISPR/Cas9 system)。TALENCRISPR分别在2012年、2013年被Science杂志评为年度十大科学进展之一。基因组定向编辑技术使得在全基因组层面上研究涉及某个完整生物学过程、信号通路、代谢途径的所有基因的功能与关系成为可能;同时也可用于研究任意基因之间的遗传学相互作用;若与有效的表型检测技术相结合,还可以针对感兴趣的科学问题进行全基因组范围的饱和筛选,而不会有基因的重复和遗漏。因此,基因组定向编辑技术使基因功能研究和改造进入了一个全新的时代。本实验室将在前期良好的工作基础上,一方面不断完善该技术,另一方面将尝试其在生物技术领域的应用。


招生信息

   
招生专业
071007-遗传学
招生方向
植物抗病免疫分子机理
作物基因组编辑技术研究

工作经历

   
工作简历
1998-01~2009-08,欧、美多个研究机构, 研究人员
社会兼职
2015-08-01-今,中国植物生理与分子生物学会-植物微生物互作专业委员会, 委员
2014-11-24-今,生物技术通报编委,
2014-10-18-2018-10-18,中国生物化学及分子生物学会农业生化与分子生物学分会, 理事
2011-02-10-今,Frontiers in Plant Sciences:Cell Biology, 编委

教授课程

微生物学讲座
微生物学系列讲座

专利与奖励

   
奖励信息
(1) 大北农科技奖, 二等奖, 其他, 2015
专利成果
[1] 邱金龙, 刘关稳, 尹康权. 改进的基因组编辑系统及其应用. CN: CN112266418A, 2021-01-26.

[2] 高晋兰, 张丹丹, 邱金龙. OsMKK6蛋白及编码基因在调控植物种子发育中的应用. CN: CN107805632B, 2019-08-30.

[3] 高晋兰, 张丹丹, 邱金龙. OsMPK4蛋白及编码基因在调控植物种子发育中的应用. CN: CN107805633B, 2019-08-30.

[4] 邱金龙, 左张丽, 田彩娟. 一种受ABA调控的miRNA在提高植物抗旱性中的应用. CN: CN105441474B, 2019-01-15.

[5] 邱金龙, 张靖波, 尹康权. 利用多肽和小分子调控水稻或小麦中蛋白质表达水平. CN: CN106317207A, 2017-01-11.

[6] 邱金龙, 苏鸓, 李盛楠. 一种多肽及其在小分子调控蛋白积累程度中的应用. CN: CN103088009A, 2013-05-08.

出版信息

   
发表论文
[1] Jin, Shuai, Lin, Qiupeng, Luo, Yingfeng, Zhu, Zixu, Liu, Guanwen, Li, Yunjia, Chen, Kunling, Qiu, JinLong, Gao, Caixia. Genome-wide specificity of prime editors in plants. NATURE BIOTECHNOLOGY[J]. 2021, 39(10): 1292-+, http://dx.doi.org/10.1038/s41587-021-00891-x.
[2] Lin, Qiupeng, Jin, Shuai, Zong, Yuan, Yu, Hong, Zhu, Zixu, Liu, Guanwen, Kou, Liquan, Wang, Yanpeng, Qiu, JinLong, Li, Jiayang, Gao, Caixia. High-efficiency prime editing with optimized, paired pegRNAs in plants. NATURE BIOTECHNOLOGY[J]. 2021, 39(8): 923-+, http://dx.doi.org/10.1038/s41587-021-00868-w.
[3] Lin, Qiupeng, Zhu, Zixu, Liu, Guanwen, Sun, Chao, Lin, Dexing, Xue, Chenxiao, Li, Shengnan, Zhang, Dandan, Gao, Caixia, Wang, Yanpeng, Qiu, JinLong. Genome editing in plants with MAD7 nuclease. JOURNAL OF GENETICS AND GENOMICS[J]. 2021, 48(6): 444-451, [4] Li, Chao, Zong, Yuan, Jin, Shuai, Zhu, Haocheng, Lin, Dexing, Li, Shengnan, Qiu, JinLong, Wang, Yanpeng, Gao, Caixia. SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds. GENOME BIOLOGY[J]. 2020, 21(1): http://dx.doi.org/10.1186/s13059-020-02051-x.
[5] Zhang, Yunwei, Ran, Yidong, Nagy, Istvan, Lenk, Ingo, Qiu, JinLong, Asp, Torben, Jensen, Christian S, Gao, Caixia. Targeted mutagenesis in ryegrass (Lolium spp.) using the CRISPR/Cas9 system. PLANT BIOTECHNOLOGY JOURNALnull. 2020, 18(9): 1854-1856, http://dx.doi.org/10.1111/pbi.13359.
[6] Zhang, Qianwei, Yin, Kangquan, Liu, Guanwen, Li, Shengnan, Li, Mengou, Qiu, JinLong. Fusing T5 exonuclease with Cas9 and Cas12a increases the frequency and size of deletion at target sites. SCIENCE CHINA-LIFE SCIENCES[J]. 2020, 63(12): 1918-1927, http://lib.cqvip.com/Qikan/Article/Detail?id=7103760293.
[7] Wang, Shengxing, Zong, Yuan, Lin, Qiupeng, Zhang, Huawei, Chai, Zhuangzhuang, Zhang, Dandan, Chen, Kunling, Qiu, JinLong, Gao, Caixia. Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC-Cas9. NATURE BIOTECHNOLOGY[J]. 2020, 38(12): https://www.webofscience.com/wos/woscc/full-record/WOS:000544158400005.
[8] Zhang, Qian, Hou, Congcong, Tian, Yudan, Tang, Mitianguo, Feng, Changxin, Ren, Zhijie, Song, Jiali, Wang, Xiaohan, Li, Tiange, Li, Mengou, Tian, Wang, Qiu, Jinlong, Liu, Liangyu, Li, Legong. Interaction Between AtCML9 and AtMLO10 Regulates Pollen Tube Development and Seed Setting. FRONTIERS IN PLANT SCIENCE[J]. 2020, 11: https://doaj.org/article/49139ecf58b24ec7a146af2a6f227911.
[9] Li, Chao, Zhang, Rui, Meng, Xiangbing, Chen, Sha, Zong, Yuan, Lu, Chunju, Qiu, JinLong, Chen, YuHang, Li, Jiayang, Gao, Caixia. Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors. NATURE BIOTECHNOLOGY[J]. 2020, 38(7): 875-U66, https://www.webofscience.com/wos/woscc/full-record/WOS:000508165600005.
[10] Yin, Kangquan, Qiu, JinLong. Genome editing for plant disease resistance: applications and perspectives. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCESnull. 2019, 374(1767): http://dx.doi.org/10.1098/rstb.2018.0322.
[11] Liu, Guanwen, Yin, Kangquan, Zhang, Qianwei, Gao, Caixia, Qiu, JinLong. Modulating chromatin accessibility by transactivation and targeting proximal dsgRNAs enhances Cas9 editing efficiency in vivo. GENOME BIOLOGY[J]. 2019, 20(1): http://dx.doi.org/10.1186/s13059-019-1762-8.
[12] Zhang, Dandan, Tian, Caijuan, Yin, Kangquan, Wang, Wenyi, Qiu, JinLong. Postinvasive Bacterial Resistance Conferred by Open Stomata in Rice. MOLECULAR PLANT-MICROBE INTERACTIONS[J]. 2019, 32(2): 255-266, [13] Jin, Shuai, Zong, Yuan, Gao, Qiang, Zhu, Zixu, Wang, Yanpeng, Qin, Peng, Liang, Chengzhi, Wang, Daowen, Qiu, JinLong, Zhang, Feng, Gao, Caixia. Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice. SCIENCE[J]. 2019, 364(6437): 292-+, [14] 陈巍, 李华丽, 邱金龙. 异源表达古菌TRAM基因增强水稻的耐旱性. 生物工程学报[J]. 2019, 1676-1685, http://lib.cqvip.com/Qikan/Article/Detail?id=83728785504849574857484949.
[15] Zhang, Jingbo, Yin, Kangquan, Sun, Juan, Gao, Jinlan, Du, Qiuli, Li, Huali, Qiu, JinLong. Direct and tunable modulation of protein levels in rice and wheat with a synthetic small molecule. PLANT BIOTECHNOLOGY JOURNAL[J]. 2018, 16(2): 472-481, http://dx.doi.org/10.1111/pbi.12787.
[16] Zong, Yuan, Song, Qianna, Li, Chao, Jin, Shuai, Zhang, Dingbo, Wang, Yanpeng, Qiu, JinLong, Gao, Caixia. Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A. NATURE BIOTECHNOLOGY[J]. 2018, 36(10): 950-+, https://www.webofscience.com/wos/woscc/full-record/WOS:000447001600014.
[17] Liang, Zhen, Chen, Kunling, Zhang, Yi, Liu, Jinxing, Yin, Kangquan, Qiu, JinLong, Gao, Caixia. Genome editing of bread wheat using biolistic delivery of CRISPR/Cas9 &ITin vitro&IT transcripts or ribonucleoproteins. NATURE PROTOCOLS[J]. 2018, 13(3): 413-430, http://dx.doi.org/10.1038/nprot.2017.145.
[18] 张丹丹, 邱金龙. 植物与病原物的相互作用及协同进化. 科学通报[J]. 2017, 62(12): 1214-1220, [19] Xiao, Xiang, Cheng, Xi, Yin, Kangquan, Li, Huali, Qiu, JinLong. Abscisic acid negatively regulates post-penetration resistance of Arabidopsis to the biotrophic powdery mildew fungus. SCIENCE CHINA-LIFE SCIENCES[J]. 2017, 60(8): 891-901, http://dx.doi.org/10.1007/s11427-017-9036-2.
[20] Zhang, Dingbo, Zhang, Huawei, Li, Tingdong, Chen, Kunling, Qiu, JinLong, Gao, Caixia. Perfectly matched 20-nucleotide guide RNA sequences enable robust genome editing using high-fidelity SpCas9 nucleases. GENOME BIOLOGY[J]. 2017, 18(1): https://doaj.org/article/36640f1a85934dc4b03db185e13b9a8c.
[21] Zong, Yuan, Wang, Yanpeng, Li, Chao, Zhang, Rui, Chen, Kunling, Ran, Yidong, Qiu, JinLong, Wang, Daowen, Gao, Caixia. Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. NATURE BIOTECHNOLOGY[J]. 2017, 35(5): 438-+, https://www.webofscience.com/wos/woscc/full-record/WOS:000400809800017.
[22] 肖翔, 程曦, 尹康权, 李华丽, 邱金龙. 脱落酸负调控拟南芥对白粉菌的穿透后抗性. 中国科学. 生命科学[J]. 2017, 47(9): 949-959, [23] Yin, Kangquan, Gao, Caixia, Qiu, JinLong. Progress and prospects in plant genome editing. NATURE PLANTSnull. 2017, 3(8): http://dx.doi.org/10.1038/nplants.2017.107.
[24] Shengnan Li, Wenyi Wang, Jinlan Gao, Kangquan Yin, Rui Wang, Chengcheng Wang, Morten Petersen, John Mundy, JinLong Qiu. MYB75 Phosphorylation by MPK4 Is Required for Light-Induced Anthocyanin Accumulation in Arabidopsis OPEN. The Plant Cell. 2016, 28(11): 2866-2883, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155340/.
[25] Yi Zhang, Zhen Liang, Yuan Zong, Yanpeng Wang, Jinxing Liu, Kunling Chen, JinLong Qiu, Caixia Gao. Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA. NATURE COMMUNICATIONS[J]. 2016, 7(1): http://www.corc.org.cn/handle/1471x/2375990.
[26] Li, Shengnan, Wang, Wenyi, Gao, Jinlan, Yin, Kangquan, Wang, Rui, Wang, Chengcheng, Petersen, Morten, Mundy, John, Qiu, JinLong. MYB75 Phosphorylation by MPK4 Is Required for Light-Induced Anthocyanin Accumulation in Arabidopsis. PLANT CELL[J]. 2016, 28(11): 2866-2883, https://www.webofscience.com/wos/woscc/full-record/WOS:000390800000012.
[27] 程曦, 王文义, 邱金龙. 基因组编辑:植物生物技术的机遇与挑战. 生物技术通报[J]. 2015, 31(4): 25-33, [28] Tian, Caijuan, Zuo, Zhangli, Qiu, JinLong. Identification and Characterization of ABA-Responsive MicroRNAs in Rice. JOURNAL OF GENETICS AND GENOMICS[J]. 2015, 42(7): 393-402, http://dx.doi.org/10.1016/j.jgg.2015.04.008.
[29] 王程程, 杜秋丽, 伍粲, 郭葳, 邱金龙. 电压依赖性阴离子通道VDAC3参与拟南芥先天免疫. 植物病理学报[J]. 2015, 45(4): 395-400, http://lib.cqvip.com/Qikan/Article/Detail?id=665528714.
[30] Wang, Yanpeng, Cheng, Xi, Shan, Qiwei, Zhang, Yi, Liu, Jinxing, Gao, Caixia, Qiu, JinLong. Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew. NATURE BIOTECHNOLOGY[J]. 2014, 32(9): 947-951, https://www.webofscience.com/wos/woscc/full-record/WOS:000342600300035.
[31] 王延鹏, 程曦, 高彩霞, 邱金龙. 利用基因组编辑技术创制抗白粉病小麦. 遗传[J]. 2014, 36(8): 848-848, http://lib.cqvip.com/Qikan/Article/Detail?id=662237492.
[32] Jin-Long Qiu. The chloride channel family gene CLCd negatively regulates PAMP-triggered immunity in Arabidopsis. Journal of Experimental Botany. 2014, [33] Guo Wei, Wang Chengcheng, Zuo Zhangli, Qiu JinLong. The roles of anion channels in Arabidopsis immunity.. Plant signaling & behavior. 2014, [34] Guo, Wei, Zuo, Zhangli, Cheng, Xi, Sun, Juan, Li, Huali, Li, Legong, Qiu, JinLong. The chloride channel family gene CLCd negatively regulates pathogen-associated molecular pattern (PAMP)-triggered immunity in Arabidopsis. JOURNAL OF EXPERIMENTAL BOTANY[J]. 2014, 65(4): 1205-1215, https://www.webofscience.com/wos/woscc/full-record/WOS:000332333000023.
[35] Shan, Qiwei, Wang, Yanpeng, Li, Jun, Zhang, Yi, Chen, Kunling, Liang, Zhen, Zhang, Kang, Liu, Jinxing, Xi, Jianzhong Jeff, Qiu, JinLong, Gao, Caixia. Targeted genome modification of crop plants using a CRISPR-Cas system. NATURE BIOTECHNOLOGYnull. 2013, 31(8): 686-688, https://www.webofscience.com/wos/woscc/full-record/WOS:000322911000010.
[36] Su, Lei, Li, Aining, Li, Huali, Chu, Chengcai, Qiu, JinLong. Direct Modulation of Protein Level in Arabidopsis. MOLECULAR PLANTnull. 2013, 6(5): 1711-1714, http://lib.cqvip.com/Qikan/Article/Detail?id=47523371.
[37] 程曦, 田彩娟, 李爱宁, 邱金龙. 植物与病原微生物互作分子基础的研究进展. 遗传[J]. 2012, 34(2): 134-144, http://lib.cqvip.com/Qikan/Article/Detail?id=41040792.
[38] Zou, Liangping, Sun, Xuehui, Zhang, Zhiguo, Liu, Peng, Wu, Jinxia, Tian, Caijuan, Qiu, Jinlong, Lu, Tiegang. Leaf Rolling Controlled by the Homeodomain Leucine Zipper Class IV Gene Roc5 in Rice. PLANT PHYSIOLOGY[J]. 2011, 156(3): 1589-1602, https://www.webofscience.com/wos/woscc/full-record/WOS:000292294100052.
[39] Jin-Long Qiu. Leaf rolling Controlled by homeodomain leucine zip class IV gene Roc5 in Rice. 2011, [40] Petersen, Klaus, Qiu, JinLong, Lutje, Juri, Fiil, Berthe Katrine, Hansen, Sidsel, Mundy, John, Petersen, Morten. Arabidopsis MKS1 Is Involved in Basal Immunity and Requires an Intact N-terminal Domain for Proper Function. PLOS ONE[J]. 2010, 5(12): https://doaj.org/article/97e771dce5ac4f8d92cf149ac817a3f8.
[41] Jin-Long Qiu. Arabidopsis MAP Kinase Kinases MKK1 and MKK2 have overlapping functions in defense signalling mediated by MEKK1, MPK4 and MKS1. Plant Physiology. 2008, [42] Jin-Long Qiu. Arabidopsis MAP Kinase 4 regulates gene expression via transcription factor release in the nucleus. EMBO Journal. 2008, [43] 邱金龙, 王隆华, 颜季琼. 棉胚珠来源单细胞的培养及部分特性初探. 作物学报[J]. 1997, 23(5): 562-, http://lib.cqvip.com/Qikan/Article/Detail?id=2649619.
[44] 邱金龙, 王隆华. 离体未受精棉胚珠和纤维分化. 上海农业学报. 1997, 13(1): 7-10, http://lib.cqvip.com/Qikan/Article/Detail?id=2465701.
[45] 王隆华, 邱金龙, 颜季琼. 棉纤维分化和发育研究进展. 植物学通报[J]. 1996, 13(2): 1-, http://lib.cqvip.com/Qikan/Article/Detail?id=2102343.

科研活动

   
科研项目
( 1 ) 植物免疫机制与作物抗病分子设计的重大基础理论, 参与, 国家级, 2011-01--2015-12
( 2 ) 农作物主要病害发生的分子机理及防控策略 , 参与, 国家级, 2011-01--2015-12
( 3 ) 国家重点实验室自主课题及运行费, 主持, 国家级, 2016-01--2016-12
( 4 ) 植物抗病信号转导, 主持, 部委级, 2011-01--2014-12
( 5 ) 水稻OsMKK1-OsMPK2级联调控抗病反应的方式与机制, 主持, 国家级, 2014-01--2017-12
( 6 ) 水稻、小麦中转基因表达蛋白水平的时空高效调控系统的建立, 主持, 国家级, 2014-01--2016-12
( 7 ) 病原相关分子模式激发的水稻抗病免疫蛋白磷酸化信号网络及其调控, 主持, 部委级, 2014-07--2018-06
( 8 ) 小麦高效基因组定向编辑技术体系的建立及应用, 参与, 国家级, 2014-01--2016-12
参与会议
(1)基因组编辑技术及其在作物育种中的应用   2015年全国植物生物学大会   邱金龙   2015-10-09
(2)Genome editing of bread wheat for heritable resistance to powdery mildew   Jin-Long Qiu   2015-08-01
(3)Editing the Wheat Genome for Disease Resistance   第23届植物动物基因组国际大会   邱金龙   2015-01-10

指导学生

已指导学生

田彩娟  01  19180  

李盛楠  01  19180  

程曦  01  19180  

王程程  02  19180  

左张丽  02  63228  

现指导学生

肖翔  01  19180  

王文义  01  19180  

张靖波  02  19180  

张丹丹  01  19180  

王曾茜  02  19180  

陈巍  01  19180  

张倩伟   02  19180  

刘关稳  01  19180