杜文斌  男  博导  中国科学院微生物研究所
电子邮件: wenbin@im.ac.cn
通信地址: 北京市朝阳区北辰西路1号院3号H1101
邮政编码: 100101

研究领域

微生物学的核心准则之一是微生物细胞的大小及其与环境作用的有效距离在微米量级。因此,利用新的技术手段来提高微生物研究的时空分辨率,在微尺度上进行微生物学研究,具有重要意义。本课题组致力于建立微生物学”芯片实验室”,从微流控器件的创新设计、制备和集成出发,通过微分离培养、单细胞分选、单细胞基因组学等新技术,研究微生物的多样性和功能,以及微生物在生态环境、感染疾病中的作用,推动微生物在资源挖掘、生物制造、基因诊断、生物安全等领域的应用。

研究涉及微生物学、生物化学、微流控学等多学科交叉。利用微流控液滴技术实现高通量的微生物单细胞分离和筛选:包括开发自动化的装置和芯片,实现大规模的单细胞分离、培养、互作和测序。利用这些新技术和开发的新平台,希望能够在环境不可培养微生物资源培养、稀有物种和极端微生物获取、潜在产酶和产活性代谢物功能菌株筛选和评价、复杂微生物群落生态功能和进化研究等方面发挥作用。此外,我们将针对临床精准诊断和生物安全现场快速检测等开展应用技术研发,并积极推动微流控技术的产业化应用。近期课题包括:

1.    微生物单细胞分离、培养和高通量分选:利用液滴微流控技术平台在单细胞水平对环境和富集培养样品中的功能菌进行高通量分选、培养和分析,加快微生物资源的收集挖掘和评估。
2.    不可培养微生物单细胞测序:结合流式细胞分选(FACS)和纳升体积单细胞基因组扩增技术,实现环境中未培养功能微生物菌群的单细胞基因组测序和功能分析。
3.    数字PCR技术方法开发和应用:利用基于震动的液滴打印技术以及高通量液滴微流控芯片搭建数字PCR技术平台,用于细菌,病毒等病原微生物的绝对定量分析。
4.    病原微生物分离、鉴定和耐药分析:使用阵列式芯片实现临床样品中病原微生物的分离和分子鉴定,以及病原菌的药物敏感性定量分析。

招生信息

欢迎具有生物学、化学、物理学、力学、生物医学工程、材料学、计算科学等背景的本科生报考。

课题组常年招收博士后和研究助理,待遇优厚,有意请发邮件至:wenbin@im.ac.cn
博士后应聘条件:
1、具有生物学、生物医学、化学、物理学、工程、生信等相关专业博士学位;
2、具有扎实的专业基础与丰富的实验经验,具有独立承担项目并开展科研工作的能力;
3、具有良好的执行力和条理性,能有效规划并推进课题进展;
4、有较强的中英文阅读写作能力,能够独立撰写研究论文;
5、身体健康,热爱科研工作,具有较强的工作责任心和团队合作精神。

研究助理(项目聘用)应聘条件:
1、具有生物学、化学、光学、流体力学、工程、计算科学等相关专业本科或专科以上学位;
2、具有良好的执行力和条理性,能有效规划并推进课题进展;
3、身体健康,热爱科研工作,具有较强的工作责任心和团队合作精神;
4、具有本市户籍或应届毕业生优先考虑。

http://www.im.cas.cn/zp2018/zjrczp/201907/t20190710_5339602.html

招生专业
071005-微生物学
0710J3-生物信息学
招生方向
微生物组学
微流控学
生化分析与临床诊断学

教育背景

2002-09--2007-06   浙江大学化学系   研究生/博士学位
1998-09--2002-07   浙江大学化学系   本科/学士

工作经历

   
工作简历
2021-09~现在, 云南大学, 生命科学学院, 联合培养硕士生导师
2021-09~现在, 山东第一医科大学, 联合培养博士生导师
2019-09~现在, 东北大学, 理学院化学系, 联合培养博士生导师
2016-09~现在, 中国科学院大学, 岗位教授
2014-09~2016-06,中国科学技术大学, 生命科学学院, 联合培养硕士生导师
2013-12~现在, 中国科学院微生物研究所, 研究员,课题组长
2011-07~2017-11,中国人民大学化学系, 特聘研究员
2007-09~2011-06,美国芝加哥大学化学系, 博士后
社会兼职
2022-11-01-今,中国初级卫生保健基金会检验医学研究与转化专业委员会, 首席科研专家
2022-10-01-今,中国化学会, 高级会员
2022-08-31-今,国家药品监督管理局药品审评中心, 外聘专家
2021-07-31-2025-08-30,中国生物物理学会,肠道菌群分会, 委员
2020-09-01-今,中华医学会微生物与免疫学分会, 委员
2017-09-01-2020-09-30,北京医学检验学会, 微生物与感染分会, 常委
2017-04-01-今,生物工程学会-生物传感、生物芯片与纳米生物技术专业委员会, 委员
2017-02-28-今,北京医学检验学会,体外诊断系统工程技术分委会, 理事

教授课程

微生物组与微生物基因组学
微生物组学与微生物基因组学
病原基因组学
分子诊断技术前沿讲座
仪器分析实验
色谱分析概论
教学经历

2011-2013: 色谱分析(研究生)

2011-2013: 仪器分析实验(本科)

专利与奖励

申请中国发明专利、中国实用新型专利、PCT及美国专利30余项,授权24项

奖励信息
(1) 海洋工程科学技术奖, 一等奖, 部委级, 2020

出版信息

   
发表论文
[1] Dongwei Chen, Mengyue Nie, Wei Tang, Yuwei Zhang, Jian Wang, Ying Lan, Yihua Chen, Wenbin Du. Whole lifecycle observation of single‐spore germinated Streptomyces using a nanogap‐stabilized microfluidic chip. mLife[J]. 2022, 1(3): 341-349, https://doaj.org/article/7ccb7917426f44c1a559835b64a1afad.
[2] Ye, Shun, Li, Caiming, Zheng, Xu, Huang, Weihang, Tao, Yi, Yu, Yanghuan, Yang, Limin, Lan, Ying, Ma, Liang, Bian, Shengtai, Du, Wenbin. OsciDrop: A Versatile Deterministic Droplet Generator. ANALYTICAL CHEMISTRY[J]. 2022, 94(6): 2918-2925, http://dx.doi.org/10.1021/acs.analchem.1c04852.
[3] Qiao, Yuxin, Hu, Ran, Chen, Dongwei, Wang, Li, Wang, Zhiyi, Yu, Haiyan, Fu, Ye, Li, Chunli, Dong, Zhiyang, Weng, YunXuan, Du, Wenbin. Fluorescence-activated droplet sorting of PET degrading microorganisms. JOURNAL OF HAZARDOUS MATERIALS[J]. 2022, 424: http://dx.doi.org/10.1016/j.jhazmat.2021.127417.
[4] JingLin Zhang, Juanli Yun, Lin Yue, Wenbin Du, YuHong Liang. Distinctive microbiota distribution from healthy oral to post-treatment apical periodontitis. Frontiers in Cellular and Infection Microbiology[J]. 2022, 12: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9492884/.
[5] Wang, ZR, Li, M, Liu, Z, Kang, WM, Cui, YZ, Han, JX, Du, WB. The oncogenic role of human microcephalin gene revealed by pan-cancer analysis. medRxiv[J]. 2022, [6] Xie, Bingliang, Wang, Jian, Nie, Yong, Tian, Jing, Wang, Zerui, Chen, Dongwei, Hu, Beiyu, Wu, Xiao-Lei, Du, Wenbin. Type IV Pili Trigger Episymbiotic Association of Saccharibacteria with Its Bacterial Host. Proceedings of the National Academy of Sciences of the United States of America[J]. 2022, 119(49): e2215990119-, [7] Feng, HC, Lv, Y, Krell, T, Fu, RX, Liu, YP, Xu, ZH, Du, WB, Shen, QR, Zhang, N, Zhang, RF. Signal binding at both modules of its dCache domain enables the McpA chemoreceptor of Bacillus velezensis to sense different ligands. Proceedings of the National Academy of Sciences of the United States of America[J]. 2022, 119(29): e2201747119-, [8] Cui, Yinglu, Chen, Yanchun, Liu, Xinyue, Dong, Saijun, Tian, Yue, Qiao, Yuxin, Mitra, Ruchira, Han, Jing, Li, Chunli, Han, Xu, Liu, Weidong, Chen, Quan, Wei, Wangqing, Wang, Xin, Du, Wenbin, Tang, Shuangyan, Xiang, Hua, Liu, Haiyan, Liang, Yong, Houk, Kendall N, Wu, Bian. Computational Redesign of a PETase for Plastic Biodegradation under Ambient Condition by the GRAPE Strategy. ACS CATALYSIS[J]. 2021, 11(3): 1340-1350, http://dx.doi.org/10.1021/acscatal.0c05126.
[9] Hu, Beiyu, Xu, Peng, Ma, Liang, Chen, Dongwei, Wang, Jian, Dai, Xin, Huang, Li, Du, Wenbin. One cell at a time: droplet-based microbial cultivation, screening and sequencing. MARINE LIFE SCIENCE & TECHNOLOGY[J]. 2021, 3(2): 169-188, http://dx.doi.org/10.1007/s42995-020-00082-8.
[10] Zheng, Xiaowei, Liu, Wang, Dai, Xin, Zhu, Yaxin, Wang, Jinfeng, Zhu, Yongqiang, Zheng, Huajun, Huang, Ying, Dong, Zhiyang, Du, Wenbin, Zhao, Fangqing, Huang, Li. Extraordinary diversity of viruses in deep-sea sediments as revealed by metagenomics without prior virion separation. ENVIRONMENTAL MICROBIOLOGY[J]. 2021, 23(2): 728-743, http://dx.doi.org/10.1111/1462-2920.15154.
[11] Yun, Juanli, Du, Wenbin. Two Metagenome-Assembled Genomes of Hydrogen-Dependent Methanomassiliicoccales Methanogens from the Zoige Wetland of the Tibetan Plateau. MICROBIOLOGY RESOURCE ANNOUNCEMENTSnull. 2021, 10(17): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086201/.
[12] Yun, Juanli, Crombie, Andrew T, Ul Haque, Muhammad Farhan, Cai, Yuanfeng, Zheng, Xiaowei, Wang, Jian, Jia, Zhongjun, Murrell, J Colin, Wang, Yanfen, Du, Wenbin. Revealing the community and metabolic potential of active methanotrophs by targeted metagenomics in the Zoige wetland of the Tibetan Plateau. ENVIRONMENTAL MICROBIOLOGY[J]. 2021, 23(11): 6520-6535, http://apps.webofknowledge.com/CitedFullRecord.do?product=UA&colName=WOS&SID=5CCFccWmJJRAuMzNPjj&search_mode=CitedFullRecord&isickref=WOS:000686297500001.
[13] 张思琦, 王剑, 兰英, 贠娟莉, 陈建, 何湘伟, 杜文斌. 抗菌活性放线菌的界面微滴移液互作分离筛选方法. 微生物学通报[J]. 2021, 48(1): 325-335, http://lib.cqvip.com/Qikan/Article/Detail?id=7104294328.
[14] Hu, Beiyu, Ye, Shun, Chen, Dongwei, Xie, Bingliang, Hu, Ran, Qiao, Yuxin, Yu, Yanghuan, Yu, Haiyan, Zheng, Xu, Lan, Ying, Du, Wenbin. Tunable and Contamination-Free Injection with Microfluidics by Stepinjection. ANALYTICAL CHEMISTRY[J]. 2021, 93(39): 13112-13117, http://dx.doi.org/10.1021/acs.analchem.1c02721.
[15] 段云峰, 王升跃, 陈禹保, 杨瑞馥, 李后开, 朱怀球, 童贻刚, 杜文斌, 付钰, 胡松年, 王军, 辛玉华, 赵方庆, 鲍一明, 张雯, 李娟, 曾明, 牛海涛, 周欣, 李岩, 崔生辉, 袁静, 李俊桦, 王加义, 刘东来, 倪铭, 孙青, 邓晔, 朱宝利. 微生物组测序与分析专家共识. 生物工程学报[J]. 2020, 36(12): 2516-2524, http://lib.cqvip.com/Qikan/Article/Detail?id=7103610501.
[16] Wang, Jian, Ruan, ChuJin, Song, Lei, Li, Ang, Zhu, YaXin, Zheng, XiaoWei, Wang, Li, Lu, ZuJun, Huang, Ying, Du, Wenbin, Zhou, Yuguang, Huang, Li, Dai, Xin. Gimesia benthica sp. nov., a planctomycete isolated from a deep-sea water sample of the Northwest Indian Ocean. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY[J]. 2020, 70(7): 4384-4389, https://www.webofscience.com/wos/woscc/full-record/WOS:000560646100043.
[17] Liu, Yunpeng, Feng, Haichao, Fu, Ruixin, Zhang, Nan, Du, Wenbin, Shen, Qirong, Zhang, Ruifu. Induced root-secreted D-galactose functions as a chemoattractant and enhances the biofilm formation of Bacillus velezensis SQR9 in an McpA-dependent manner. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2020, 104(2): 785-797, http://dx.doi.org/10.1007/s00253-019-10265-8.
[18] Hu, Beiyu, Xu, Bingxue, Yun, Juanli, Wang, Jian, Xie, Bingliang, Li, Caiming, Yu, Yanghuan, Lan, Ying, Zhu, Yaxin, Dai, Xin, Huang, Ying, Huang, Li, Pan, Jianzhang, Du, Wenbin. High-throughput single-cell cultivation reveals the underexplored rare biosphere in deep-sea sediments along the Southwest Indian Ridge. LAB ON A CHIP[J]. 2020, 20(2): 363-372, https://www.webofscience.com/wos/woscc/full-record/WOS:000508846700012.
[19] Ruan, ChuJin, Wang, Jian, Zheng, XiaoWei, Song, Lei, Zhu, YaXin, Huang, Ying, Lu, ZuJun, Du, WenBin, Huang, Li, Dai, Xin. Halovulum marinum sp. nov., isolated from deep-sea water of the Indian Ocean, and emended description of the genus Halovulum. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY[J]. 2020, 70(8): 4523-4530, http://dx.doi.org/10.1099/ijsem.0.004307.
[20] Qu, Haijun, Yu, Mengchao, Du, Wenbin, Xu, Lei, Lyu, Weiyuan, Shen, Feng. Slip Molding for Precision Fabrication of Microparts. LANGMUIR[J]. 2020, 36(2): 585-590, https://www.webofscience.com/wos/woscc/full-record/WOS:000509420200013.
[21] Liu, Yaoyao, Xu, Jingjing, Tao, Yi, Fang, Teng, Du, Wenbin, Ye, Anpei. Rapid and accurate identification of marine microbes with single-cell Raman spectroscopy. ANALYST[J]. 2020, 145(9): 3297-3305, https://www.webofscience.com/wos/woscc/full-record/WOS:000532257200010.
[22] Yun, Juanli, Zheng, Xiaowei, Xu, Peng, Zheng, Xu, Xu, Jingyue, Cao, Chen, Fu, Yusi, Xu, Bingxue, Dai, Xin, Wang, Yi, Liu, Hongtao, Yi, Qiaolian, Zhu, Yaxin, Wang, Jian, Wang, Li, Dong, Zhiyang, Huang, Li, Huang, Yanyi, Du, Wenbin. Interfacial Nanoinjection-Based Nanoliter Single-Cell Analysis. SMALL[J]. 2020, 16(9): https://www.webofscience.com/wos/woscc/full-record/WOS:000488077200001.
[23] Tao, Yi, Yun, Juanli, Wang, Jian, Xu, Peng, Li, Caiming, Liu, Hongtao, Lan, Ying, Pan, Jianzhang, Du, Wenbin. High-performance detection of Mycobacterium bovis in milk using digital LAMP. FOOD CHEMISTRY[J]. 2020, 327: http://dx.doi.org/10.1016/j.foodchem.2020.126945.
[24] Chen, Dongwei, Liu, ShuangJiang, Du, Wenbin. Chemotactic screening of imidazolinone-degrading bacteria by microfluidic SlipChip. JOURNAL OF HAZARDOUS MATERIALS[J]. 2019, 366: 512-519, http://dx.doi.org/10.1016/j.jhazmat.2018.12.029.
[25] Nie, Mengyue, Zheng, Meng, Li, Caiming, Shen, Feng, Liu, Manhua, Luo, Haibei, Song, Xiaohui, Lan, Ying, Pan, Jianzhang, Du, Wenbin. Assembled Step Emulsification Device for Multiplex Droplet Digital PCR. Analytical Chemistry[J]. 2019, 91: 1779-1784, [26] Feng, Haichao, Zhang, Nan, Fu, Ruixin, Liu, Yunpeng, Krell, Tino, Du, Wenbin, Shao, Jiahui, Shen, Qirong, Zhang, Ruifu. Recognition of dominant attractants by key chemoreceptors mediates recruitment of plant growth-promoting rhizobacteria. ENVIRONMENTAL MICROBIOLOGY[J]. 2019, 21(1): 402-415, https://www.webofscience.com/wos/woscc/full-record/WOS:000456278900028.
[27] Lyu, Weiyuan, Yu, Mengchao, Qu, Haijun, Yu, Ziqing, Du, Wenbin, Shen, Feng. Slip-driven microfluidic devices for nucleic acid analysis. BIOMICROFLUIDICSnull. 2019, 13(4): https://www.webofscience.com/wos/woscc/full-record/WOS:000483884200009.
[28] Yi, Qiaolian, Cai, Dongyang, Xiao, Meng, Nie, Mengyue, Cui, Qinna, Cheng, Jingwei, Li, Caiming, Feng, Jie, Urban, Gerald, Xu, YingChun, Lan, Ying, Du, Wenbin. Direct antimicrobial susceptibility testing of bloodstream infection on SlipChip. BIOSENSORS & BIOELECTRONICS[J]. 2019, 135: 200-207, http://dx.doi.org/10.1016/j.bios.2019.04.003.
[29] Wei, Weipeng, Meng, Cui, Wang, Yuhe, Huang, Yongsheng, Du, Wenbin, Li, Hongfang, Liu, Yanfei, Song, Hong, Tang, Fushan. The interaction between self-assembling peptides and emodin and the controlled release of emodin from in-situ hydrogel. ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY[J]. 2019, 47(1): 3961-3975, https://www.webofscience.com/wos/woscc/full-record/WOS:000488464300001.
[30] Qiaolian Yi, Dongyang Cai, Meng Xiao, Mengyue Nie, Qinna Cui, Jingwei Cheng, Caiming Li, Jie Feng, Gerald Urban, YingChun Xu, Ying Lan, Wenbin Du. Direct antimicrobial susceptibility testing of bloodstream infection on SlipChip. Biosensors and Bioelectronics. 2019, 135: 200-207, http://dx.doi.org/10.1016/j.bios.2019.04.003.
[31] Nie, Mengyue, Zheng, Meng, Li, Caiming, Shen, Feng, Liu, Manhua, Luo, Haibei, Song, Xiaohui, Lan, Ying, Pan, JianZhang, Du, Wenbin. Assembled Step Emulsification Device for Multiplex Droplet Digital Polymerase Chain Reaction. ANALYTICAL CHEMISTRY[J]. 2019, 91(3): 1779-1784, https://www.webofscience.com/wos/woscc/full-record/WOS:000458220300022.
[32] Zhukov, Dmitriy V, Khorosheva, Eugenia M, Khazaei, Tahmineh, Du, Wenbin, Selck, David A, Shishkin, Alexander A, Ismagilov, Rustem F. Microfluidic SlipChip device for multistep multiplexed biochemistry on a nanoliter scale. LAB ON A CHIP[J]. 2019, 19(19): 3200-3211, [33] Zhou, Nan, Sun, YuTong, Chen, DongWei, Du, Wenbin, Yang, Hong, Liu, ShuangJiang. Harnessing microfluidic streak plate technique to investigate the gut microbiome of Reticulitermes chinensis. MICROBIOLOGYOPEN[J]. 2019, 8(3): [34] Jin, Zengjun, Nie, Mengyue, Hu, Ran, Zhao, Tianhu, Xu, Jingyue, Chen, Dongwei, Yun, Juanli, Ma, Luyan Z, Du, Wenbin. Dynamic Sessile-Droplet Habitats for Controllable Cultivation of Bacterial Biofilm. SMALL[J]. 2018, 14(22): http://dx.doi.org/10.1002/smll.201800658.
[35] Liao, Shenglong, Tao, Yi, Du, Wenbin, Wang, Yapei. Interfacial Emulsification: An Emerging Monodisperse Droplet Generation Method for Microreactors and Bioanalysis. LANGMUIR[J]. 2018, 34(39): 11655-11666, https://www.webofscience.com/wos/woscc/full-record/WOS:000446543000002.
[36] Wang, Siyu, Wang, Xiayan, Wang, Lin, Pu, Qiaosheng, Du, Wenbin, Guo, Guangsheng. Plasma-assisted alignment in the fabrication of microchannel-array-based in-tube solid-phase microextraction microchips packed with TiO2 nanoparticles for phosphopeptide analysis. ANALYTICA CHIMICA ACTA[J]. 2018, 1018: 70-77, http://dx.doi.org/10.1016/j.aca.2018.02.018.
[37] 杜文斌. Virgibacillus indicus sp. nov. and Virgibacillus profundi sp. nov, two moderately halophilic bacteria isolated from marine sediment by using the microfluidic streak plates. International Journal of Systematic and Evolutionary Microbiology. 2018, [38] Xu, Bingxue, Hu, Beiyu, Wang, Jian, Lan, Ying, Zhu, Yaxin, Dai, Xin, Huang, Li, Huang, Ying, Du, Wenbin. Virgibacillus indicus sp nov and Virgibacillus profundi sp nov, two moderately halophilic bacteria isolated from marine sediment by using microfluidic streak plates. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY[J]. 2018, 68(6): 2015-2023, https://www.webofscience.com/wos/woscc/full-record/WOS:000434151600030.
[39] Qiao, Yuxin, Zha, Xiaoyan, Zhu, Jun, Tu, Ran, Dong, Libing, Wang, Li, Dong, Zhiyang, Wan, Qinhong, Du, Wenbin. Fluorescence-activated droplet sorting of lipolytic microorganisms using a compact optical system. LAB ON A CHIP[J]. 2018, 18(1): 190-196, https://www.webofscience.com/wos/woscc/full-record/WOS:000418367800017.
[40] 杜文斌. 抗菌药物敏感性快速检测方法研究进展. 中国感染控制杂志. 2018, [41] Ruan, ChuJin, Zheng, XiaoWei, Wang, Jian, Song, Lei, Zhu, YaXin, Du, WenBin, Lu, ZuJun, Huang, Ying, Huang, Li, Dai, Xin. Hyphobacterium indicum sp. nov., isolated from deep seawater, and emended description of the genus Hyphobacterium. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY[J]. 2018, 68(12): 3760-3765, https://www.webofscience.com/wos/woscc/full-record/WOS:000453513900012.
[42] Cai, Dongyang, Yi, Qiaolian, Shen, Chaohua, Lan, Ying, Urban, Gerald, Du, Wenbin. Direct enrichment of pathogens from physiological samples of high conductivity and viscosity using H-filter and positive dielectrophoresis. BIOMICROFLUIDICS[J]. 2018, 12(1): https://www.webofscience.com/wos/woscc/full-record/WOS:000427001300013.
[43] Feng, Haichao, Zhang, Nan, Du, Wenbin, Zhang, Huihui, Liu, Yunpeng, Fu, Ruixin, Shao, Jiahui, Zhang, Guishan, Shen, Qirong, Zhang, Ruifu. Identification of Chemotaxis Compounds in Root Exudates and Their Sensing Chemoreceptors in Plant-Growth-Promoting Rhizobacteria Bacillus amyloliquefaciens SQR9. MOLECULAR PLANT-MICROBE INTERACTIONS[J]. 2018, 31(10): 995-1005, https://www.webofscience.com/wos/woscc/full-record/WOS:000445028000003.
[44] Zhai, Zhimin, Nie, Mengyue, Guan, Yong, Zhang, Fengqiu, Chen, Liang, Muhammad, Du, Wenbin, Liu, Gang, Tian, Yangchao, Huang, Qing. A microfluidic surface-enhanced Raman spectroscopy approach for assessing the particle number effect of AgNPs on cytotoxicity. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY[J]. 2018, 162: 529-535, http://dx.doi.org/10.1016/j.ecoenv.2018.07.026.
[45] Liao, Shenglong, Tao, Xinglei, Ju, Yingjiao, Feng, Jie, Du, Wenbin, Wang, Yapei. Multichannel Dynamic Interfacial Printing: An Alternative Multicomponent Droplet Generation Technique for Lab in a Drop. ACS APPLIED MATERIALS & INTERFACES[J]. 2017, 9(50): 43545-43552, https://www.webofscience.com/wos/woscc/full-record/WOS:000418783700016.
[46] Hu, Yi, Xu, Peng, Luo, Jing, He, Hongxuan, Du, Wenbin. Absolute Quantification of H5-Subtype Avian Influenza Viruses Using Droplet Digital Loop-Mediated Isothermal Amplification. ANALYTICAL CHEMISTRY[J]. 2017, 89(1): 745-750, https://www.webofscience.com/wos/woscc/full-record/WOS:000391346600061.
[47] 贾仲君, 蔡元锋, 贠娟莉, 杜文斌. 单细胞、显微计数和高通量测序典型水稻土微生物组的技术比较. 微生物学报[J]. 2017, 57(6): 899-919, [48] Liao, Shenglong, He, Yonglin, Wang, Dingguan, Dong, Libing, Du, Wenbin, Wang, Yapei. Dynamic Interfacial Printing for Monodisperse Droplets and Polymeric Microparticles. ADVANCED MATERIALS TECHNOLOGIES[J]. 2016, 1(1): https://www.webofscience.com/wos/woscc/full-record/WOS:000398994600008.
[49] 杜文斌. Cross-Interface Emulsification for Generating Size-Tuneable Droplets. Analytical Chemistry. 2016, [50] Jiang, ChengYing, Dong, Libing, Zhao, JianKang, Hu, Xiaofang, Shen, Chaohua, Qiao, Yuxin, Zhang, Xinyue, Wang, Yapei, Ismagilov, Rustem F, Liu, ShuangJiang, Du, Wenbin. High-Throughput Single-Cell Cultivation on Microfluidic Streak Plates. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2016, 82(7): 2210-2218, https://www.webofscience.com/wos/woscc/full-record/WOS:000373342400024.
[51] 兰英, 杜文斌. 国家重点研发计划《新一代高通量数字PCR关键技术及应用研究》简介. 中国医疗器械信息[J]. 2016, 90-92, http://lib.cqvip.com/Qikan/Article/Detail?id=90718188504849545051485056.
[52] Xu, Peng, Zheng, Xu, Tao, Yi, Du, Wenbin. Cross-Interface Emulsification for Generating Size-Tunable Droplets. ANALYTICAL CHEMISTRY[J]. 2016, 88(6): 3171-3177, http://www.irgrid.ac.cn/handle/1471x/1093158.
[53] Dong, Libing, Chen, DongWei, Liu, ShuangJiang, Du, Wenbin. Automated Chemotactic Sorting and Single-cell Cultivation of Microbes using Droplet Microfluidics. SCIENTIFIC REPORTS[J]. 2016, 6: https://www.webofscience.com/wos/woscc/full-record/WOS:000374034300001.
[54] Liu, Xiao, Yi, Qiaolian, Han, Yongzhen, Liang, Zhenning, Shen, Chaohua, Zhou, Zhengyang, Sun, Junliang, Li, Yizhi, Du, Wenbin, Cao, Rui. A Robust Microfluidic Device for the Synthesis and Crystal Growth of Organometallic Polymers with Highly Organized Structures. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION[J]. 2015, 54(6): 1846-1850, https://www.webofscience.com/wos/woscc/full-record/WOS:000349209200029.
[55] Huang, Xiaopeng, Yang, Youdi, Shi, Jiezhong, Ngo, Huynh Thien, Shen, Chaohua, Du, Wenbin, Wang, Yapei. High-Internal-Phase Emulsion Tailoring Polymer Amphiphilicity towards an Efficient NIR-Sensitive Bacteria Filter. SMALL[J]. 2015, 11(37): 4876-4883, https://www.webofscience.com/wos/woscc/full-record/WOS:000362819400010.
[56] Jia, Hanyu, He, Yonglin, Zhang, Xinyue, Du, Wenbin, Wang, Yapei. Integrating Ultra-Thermal-Sensitive Fluids into Elastomers for Multifunctional Flexible Sensors. ADVANCED ELECTRONIC MATERIALS[J]. 2015, 1(3): https://www.webofscience.com/wos/woscc/full-record/WOS:000357653900008.
[57] Yu, Shan, Su, Tiantian, Wu, Huijun, Liu, Shiheng, Wang, Di, Zhao, Tianhu, Jin, Zengjun, Du, Wenbin, Zhu, MeiJun, Chua, Song Lin, Yang, Liang, Zhu, Deyu, Gu, Lichuan, Ma, Luyan Z. PslG, a self-produced glycosyl hydrolase, triggers biofilm disassembly by disrupting exopolysaccharide matrix. CELL RESEARCH[J]. 2015, 25(12): 1352-1367, https://www.webofscience.com/wos/woscc/full-record/WOS:000365808300009.
[58] Wu, ZengQiang, Du, WenBin, Li, JinYi, Xia, XingHua, Fang, Qun. Establishment of a finite element model for extracting chemical reaction kinetics in a micro-flow injection system with high throughput sampling. TALANTA[J]. 2015, 140: 176-182, http://dx.doi.org/10.1016/j.talanta.2015.03.051.
[59] Wang, Shujun, Chen, Suming, Wang, Jianing, Xu, Peng, Luo, Yuanming, Nie, Zongxiu, Du, Wenbin. Interface solution isoelectric focusing with in situ MALDI-TOF mass spectrometry. ELECTROPHORESIS[J]. 2014, 35(17): 2528-2533, http://dx.doi.org/10.1002/elps.201400083.
[60] Cai, Dongyang, Xiao, Meng, Xu, Peng, Xu, YingChun, Du, Wenbin. An integrated microfluidic device utilizing dielectrophoresis and multiplex array PCR for point-of-care detection of pathogens. LAB ON A CHIP[J]. 2014, 14(20): 3917-3924, https://www.webofscience.com/wos/woscc/full-record/WOS:000342866400003.
[61] 袁会领. 液滴微流控芯片系统中微液滴特性表征及氨基酸检测应用. 生物工程学报[J]. 2014, 30():(1): 139-146, http://124.16.173.210/handle/311007/694.
[62] Shen, Chaohua, Xu, Peng, Huang, Zhou, Cai, Dongyang, Liu, ShuangJiang, Du, Wenbin. Bacterial chemotaxis on SlipChip. LAB ON A CHIP[J]. 2014, 14(16): 3074-3080, https://www.webofscience.com/wos/woscc/full-record/WOS:000339470400024.
[63] Huang, Xiaopeng, Qian, Qiuping, Zhang, Xinyue, Du, Wenbin, Xu, Huaping, Wang, Yapei. Assembly of Carbon Nanotubes on Polymer Particles: Towards Rapid Shape Change by Near-Infrared Light. PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION[J]. 2013, 30(3): 235-240, https://www.webofscience.com/wos/woscc/full-record/WOS:000316700300003.
[64] Wang, Jian, Zhang, Xinyue, Huang, Xiaopeng, Wang, Shujun, Qian, Qiuping, Du, Wenbin, Wang, Yapei. Forced Assembly of Water-Dispersible Carbon Nanotubes Trapped in Paper for Cheap Gas Sensors. SMALL[J]. 2013, 9(22): 3759-3764, https://www.webofscience.com/wos/woscc/full-record/WOS:000336083000006.
[65] Kim, Hyun Jung, Du, Wenbin, Ismagilov, Rustem F. Complex function by design using spatially pre-structured synthetic microbial communities: degradation of pentachlorophenol in the presence of Hg(II). INTEGRATIVE BIOLOGY[J]. 2011, 3(2): 126-133, https://www.webofscience.com/wos/woscc/full-record/WOS:000287091700006.
[66] Shen, Feng, Davydova, Elena K, Du, Wenbin, Kreutz, Jason E, Piepenburg, Olaf, Ismagilov, Rustem F. Digital Isothermal Quantification of Nucleic Acids via Simultaneous Chemical Initiation of Recombinase Polymerase Amplification Reactions on SlipChip. ANALYTICAL CHEMISTRY[J]. 2011, 83(9): 3533-3540, https://www.webofscience.com/wos/woscc/full-record/WOS:000289956800046.
[67] Gu, ShuQing, Zhang, YunXia, Zhu, Ying, Du, WenBin, Yao, Bo, Fang, Qun. Multifunctional Picoliter Droplet Manipulation Platform and Its Application in Single Cell Analysis. ANALYTICAL CHEMISTRY[J]. 2011, 83(19): 7570-7576, http://dx.doi.org/10.1021/ac201678g.
[68] Shen, Feng, Sun, Bing, Kreutz, Jason E, Davydova, Elena K, Du, Wenbin, Reddy, Poluru L, Joseph, Loren J, Ismagilov, Rustem F. Multiplexed Quantification of Nucleic Acids with Large Dynamic Range Using Multivolume Digital RT-PCR on a Rotational SlipChip Tested with HIV and Hepatitis C Viral Load. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY[J]. 2011, 133(44): 17705-17712, https://www.webofscience.com/wos/woscc/full-record/WOS:000296312200038.
[69] Pompano, Rebecca R, Liu, Weishan, Du, Wenbin, Ismagilov, Rustem F, Cooks, RG, Yeung, ES. Microfluidics Using Spatially Defined Arrays of Droplets in One, Two, and Three Dimensions. ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 4[J]. 2011, 4: 59-81, https://www.webofscience.com/wos/woscc/full-record/WOS:000293148400004.
[70] Kreutz, Jason E, Munson, Todd, Huynh, Toan, Shen, Feng, Du, Wenbin, Ismagilov, Rustem F. Theoretical Design and Analysis of Multivolume Digital Assays with Wide Dynamic Range Validated Experimentally with Microfluidic Digital PCR. ANALYTICAL CHEMISTRY[J]. 2011, 83(21): 8158-8168, https://www.webofscience.com/wos/woscc/full-record/WOS:000296225300019.
发表著作
(1) "Microfluidic techniques for high-throughput cell analysis." In Biomimetic Microengineering, CRC Taylor & Francis, 2019-12, 第 5 作者
(2) 基于液滴微流控的微生物单细胞高通量培养分选技术(第12章第二节). 中国土壤微生物组, 浙江大学出版社, 2021-12, 第 5 作者

科研活动

   
科研项目
( 1 ) 基于微流控芯片的土壤微生物单细胞高通量培养技术, 负责人, 中国科学院计划, 2015-01--2018-12
( 2 ) 用于细菌耐药快速检测的微流控芯片的研制, 负责人, 地方任务, 2016-07--2018-12
( 3 ) 生物高通量检测分析服务网络:液滴微流控检测分选平台及应用, 负责人, 中国科学院计划, 2016-01--2018-12
( 4 ) 阵列微流控技术在血感染病原微生物快速培养、分离和鉴定中的应用, 负责人, 国家任务, 2015-01--2018-12
( 5 ) 新一代高通量数字PCR关键技术及应用研究, 负责人, 国家任务, 2016-07--2018-12
( 6 ) 中国科学院前沿科学重点研究项目,新型微尺度单细胞菌株选育体系与应用, 负责人, 中国科学院计划, 2016-08--2020-12
( 7 ) 人体与环境健康的微生物组共性技术研究项目, 参与, 中国科学院计划, 2017-10--2019-09
( 8 ) 广谱型化学毒剂的绿色生物洗消材料, 负责人, 国家任务, 2017-10--2020-12
( 9 ) “战略性国际科技创新合作重点专项”,人、畜传染性疾病预防监控技术, 参与, 国家任务, 2017-10--2019-12
( 10 ) 国际海域资源调查与开发“十三五”, 深海稀有微生物多样性及其菌种与基因资源获取和评价, 参与, 国家任务, 2016-01--2020-09
( 11 ) 东南大学生物电子学国家重点实验室开放研究基金课题, 基于微滴式数字PCR的病毒载量定量检测与分析, 负责人, 国家任务, 2017-01--2018-12
( 12 ) 微流控微生物组分析, 负责人, 国家任务, 2019-01--2021-12
( 13 ) 海洋未培养甲基营养菌群驱动C1循环的微流控组学分析, 负责人, 国家任务, 2020-01--2022-12

合作情况

与国家纳米科学中心、北京大学生物动态光学成像中心、北京协和医院、北京大学口腔医院、北京大学第三医院、东南大学生物电子学国家重点实验室、上海交通大学、浙江大学化学系、中科院南京土壤学研究所、中科院青岛能源与过程研究所、国家海洋局第二海洋研究所、农业部沼气科学研究所等建立了科研和项目合作。

项目协作单位
Zhiyang Dong, Institute of Microbiology, CAS

Bian Wu, Institute of Microbiology, CAS
Jie Feng, Institute of Microbiology, CAS
Yihua Chen, Institute of Microbiology, CAS
Shuang-Jiang Liu, Institute of Microbiology, CAS
Luyan Ma, Institute of Microbiology, CAS
Li Huang, Institute of Microbiology, CAS
Yapei Wang, Renmin University, Chemistry
Feng Shen, Shanghai Jiao Tong University, Biomedical
Jiashu Sun, National Center for Nanoscience and Technology, CAS
Yingchun Xu, Peking Union Medical College Hospital
Qiwen Yang, Peking Union Medical College Hospital
Rustem Ismagilov, California Institute of Technology
Novozymes

指导学生

已指导学生

奕巧莲  博士研究生  071005-微生物学  

许晶月  硕士研究生  071005-微生物学  

陶艺  博士研究生  071005-微生物学  

聂梦月  硕士研究生  085238-生物工程  

靳增军  博士研究生  071007-遗传学  

徐冰雪  硕士研究生  071005-微生物学  

聂梦月  博士研究生  071005-微生物学  

乔雨歆  博士研究生  071005-微生物学  

胡然  硕士研究生  085238-生物工程  

郑蒙  硕士研究生  085238-生物工程  

李埰明  硕士研究生  071005-微生物学  

胡倍瑜  博士研究生  071005-微生物学  

于海燕  硕士研究生  071005-微生物学  

现指导学生

谢冰亮  博士研究生  071005-微生物学  

武斌杰  博士研究生  100705-微生物与生化药学  

汪之一  博士研究生  071005-微生物学  

吴晓琳  硕士研究生  071005-微生物学  

朱晓雅  硕士研究生  086000-生物与医药  

李埰明  博士研究生  071005-微生物学  

郭思思  博士研究生  071005-微生物学