基本信息
窦硕星 男 汉族 博导 中国科学院物理所
电子邮件：sxdou@iphy.ac.cn
联系电话：(10) 8264 9484
手机号码：
通信地址：北京中国科学院物理研究所
邮政编码：100190

研究领域

复杂系统和生物大分子动力学

目前我们的研究课题主要包括：（1）DNA解旋酶等生物马达蛋白运动机理；（2）细胞内生物大分子的扩散与运输；（3）染色质动态结构和调控的分子机制；（4）抗癌药物与DNA、活细胞的相互作用。

教育背景

1990-04--1992-11   法国居里大学   理学博士
1988-09--1990-04   山东大学晶体材料研究所   博士研究生
1985-09--1988-07   山东大学晶体材料研究所   硕士
1981-09--1985-07   山东大学光学系   学士
学历

1981年9月—1985年7月      山东大学光学系，技术光学专业，本科
1985年9月—1988年7月      山东大学晶体材料研究所，固体物理专业，硕士研究生
1988年9月—1990年4月      山东大学晶体材料研究所，固体物理专业，博士研究生
1990年4月—1992年11月    法国国家电讯研究中心 (CNET)，法国居里大学（巴黎六大），物理专业，博士研究生

出国学习工作

1995年9月—1995年11月    柏林工业大学光学研究所德国马普访问学者
1997年7月—1998年1月      韩国国立江源大学物理系合作访问
2000年1月—2000年3月      柏林工业大学光学研究所德国马普访问学者
2000年4月—2001年9月      Cachan高师法国科研中心 (CNRS) 访问教授

教授课程

光学
普通物理Ⅳ-B

发表论文

     生物物理方面代表性论文

  1. C. Jiang, H.-Y. Luo, X. Xu, S.-X. Dou, W. Li, D. Guan, F. Ye, X. Chen, M. Guo, P.-Y. Wang, and H. Li, “Switch of cell migration modes orchestrated by changes of three-dimensional lamellipodium structure and intracellular diffusion.”  Nature Communications 14,  5166 (2023).
  2. C. Jiang, M.C. Yang, W. Li, S.-X. Dou, P.-Y. Wang, and H. Li, “Spatiotemporal three-dimensional transport dynamics of endocytic cargos and their physical regulations in cells.” iScience 25, 104210 (2022).
  3. Y.-Z. Wang, C. Liu, J. Zhao, J. Yu, A. Luo, X. Xiao, S.-X. Dou, L. Ma, P.-Y.Wang, M. Li, G. Li, J. Yan, P. Chen, and W. Li, “H2A mono-ubiquitination differentiates FACT's functions in nucleosome assembly and disassembly.”  Nucleic Acids Res. 50, 833-846 (2022).
  4. J.-W. Yuan, Y.-N. Zhang, Y.-R. Liu, W. Li, S.-X. Dou, Y. Wei, P.-Y. Wang, and H. Li, “Diffusion behaviors of integrins in single cells altered by epithelial to mesenchymal transition.” Small 18, 2106498 (2022).
  5. Y.-X. Dai, W.-F. Chen, N.-N. Liu, F.-Y. Teng, H.-L. Guo, X.-M. Hou, S.-X. Dou, S. Rety, and X.-G. Xi, “Structural and functional studies of SF1B Pif1 from Thermus oshimai reveal dimerization-induced helicase inhibition.” Nucleic Acids Res. 49, 4129-4143 (2021).
  6. L.-T. Zhai, S. Rety, W.-F. Chen, Z.-Y. Song, D. Auguin, B. Sun, S.-X. Dou, and X.-G. Xi, “Crystal structures of N-terminally truncated telomerase reverse transcriptase from fungi.” Nucleic Acids Res. 49, 4768-4781 (2021).
  7. N.-N. Liu, Z.-Y. Song, H.-L. Guo, H. Yin, W.-F. Chen, Y.-X. Dai, B.-G. Xin, X. Ai, L. Ji, Q.-M. Wang, X.-M. Hou, S.-X. Dou, S. Rety, and X.-G. Xi, “Endogenous Bos taurus RECQL is predominantly monomeric and more active than oligomers.” Cell Rep. 36, 109688 (2021).
  8. K. Lu, C. Liu, Y. Liu, A. Luo, J. Chen, Z. Lei, J. Kong, X. Xiao, S. Zhang, Y.-Z. Wang, L. Ma, S.-X. Dou, P.-Y. Wang, M. Li, G. Li, W. Li, and P. Chen, “Curaxin-Induced DNA Topology Alterations Trigger the Distinct Binding Response of CTCF and FACT at the Single-Molecule Level.” Biochemistry 60, 494-499 (2021).
  9. L. Dai, X. Xiao, L. Pan, L. Shi, N. Xu, Z. Zhang, X. Feng, L. Ma, S.-X. Dou, P.-Y. Wang,  B. Zhu, W. Li, and Z. Zhou, “Recognition of the inherently unstable H2A nucleosome by Swc2 is a major determinant for unidirectional H2A.Z exchange.” Cell Rep. 35, 109183 (2021).
  10. S. Zhang, X. Xiao, J. Kong, K. Lu, S.-X. Dou, P.-Y. Wang, L. Ma, Y. Liu, G. Li, W. Li, and H. Zhang, “DNA polymerase Gp90 activities and regulations on strand displacement DNA synthesis revealed at single-molecule level.” FASEB J. 35, e21607 (2021).
  11. X. Xiao, C. Liu, Y. Pei, Y.-Z. Wang, J. Kong, K. Lu, L. Ma, S.-X. Dou, P.-Y. Wang, G. Li, P. Chen, and W. Li, “Histone H2A ubiquitination reinforces mechanical stability and asymmetry at the single-nucleosome level.” J. Am. Chem. Soc. 142, 3340-3345 (2020).
  12. F.-Y. Teng, T.-T. Wang, H.-L. Guo, B.-G. Xin, B. Sun, S.-X. Dou, X.-G. Xi, and X.-M. Hou, “The HRDC domain oppositely modulates the unwinding activity of E. coli RecQ helicase on duplex DNA and G-quadruplex.” J. Biol. Chem. 295, 17646-17658 (2020).
  13. C. Jiang, B. Li, S.-X. Dou, P.-Y. Wang, and H. Li, “Quasi-two-dimensional diffusion in adherent cells revealed by three-dimensional single quantum dot tracking.” Chin. Phys. Lett. (Express Letter) 37, 078701 (2020).
  14. W.-F. Chen, X.-B. Wei, S. Rety, L.-Y. Huang, N.-N. Liu, S.-X. Dou, and X.-G. Xi, “Structural analysis reveals a “molecular calipers” mechanism for a LATERAL ORGAN BOUNDARIES DOMAIN transcription factor protein from wheat.” J. Biol. Chem. 294, 142-156 (2019).
  15. B. Li, S.-X. Dou, J.-W. Yuan, Y.-R. Liu, W. Li, F. F. Ye, P.-Y. Wang, and H. Li, “Intracellular transport is accelerated in early apoptotic cells.” Proc. Natl. Acad. Sci. U.S.A. 115, 12118-12123 (2018).
  16. W.-F. Chen, S. Rety, H.-L. Guo, Y.-X. Dai, W.-Q. Wu, N.-N. Liu, D. Auguin, Q.-W. Liu, X.-M. Hou, S.-X. Dou, and X.-G. Xi, “Molecular mechanistic insights into Drosophila DHX36-mediated G-quadruplex unfolding: A structure-based model.” Structure 26, 403-415 (2018).
  17. X.-M. Lu, H. Li, J. You, W. Li, P.-Y. Wang, M. Li, S.-X. Dou, and X.-G. Xi, “Folding dynamics of parallel and antiparallel G-triplexes under the influence of proximal DNA.” J. Phys. Chem. B 122, 9499-9506 (2018).
  18. K.-Y. Lu, W.-F. Chen, S. Rety, N.-N. Liu, W.-Q. Wu, Y.-X. Dai, D. Li, H.-Y. Ma, S.-X. Dou, and X.-G. Xi, “Insights into the structural and mechanistic basis of multifunctional S. cerevisiae Pif1p helicase.” Nucleic Acids Res. 46, 1486-1500 (2018).
  19. J. You, H. Li, X.-M. Lu, W. Li, P.-Y. Wang, S.-X. Dou, and X.-G. Xi, “Effects of monovalent cations on folding kinetics of G-quadruplexes.” Bioscience Reports 37, BSR20170771 (2017).
  20. W. X. Lin, J. B. Ma, D. G. Nong, C. H. Xu, B. Zhang, J. H. Li, Q. Jia, S.-X. Dou, F. F. Ye, X.-G. Xi, Y. Lu, and M. Li, “Helicase stepping investigated with one-nucleotide resolution fluorescence resonance energy transfer.” Phys. Rev. Lett. 119, 138102 (2017).
  21. W.-Q. Wu, X.-M. Hou, B. Zhang, P. Fossé, B. René, O. Mauffret, M. Li, S.-X. Dou, and X.-G. Xi, “Single-molecule studies reveal reciprocating of WRN helicase core along ssDNA during DNA unwinding.” Scientific Reports 7, 43954 (2017).
  22. W.-F. Chen, Y.-X. Dai, X.-L. Duan, N.-N. Liu, W. Shi, N. Li, M. Li, S.-X. Dou, Y.-H. Dong, S. Rety, and X.-G. Xi, “Crystal structures of the BsPif1 helicase reveal that a major movement of the 2B SH3 domain is required for DNA unwinding.” Nucleic Acids Res. 44, 2949-2961 (2016).
  23. B. Zhang, W.-Q. Wu, N.-N. Liu, X.-L. Duan, M. Li, S.-X. Dou, X.-M. Hou, and X.-G. Xi, “G-quadruplex and G-rich sequence stimulate Pif1p-catalyzed downstream duplex DNA unwinding through reducing waiting time at ss/dsDNA junction.” Nucleic Acids Res. 44, 8385-8394 (2016).
  24. J.-H. Li, W.-X. Lin, B. Zhang, D.-G. Nong, H.-P. Ju, J.-B. Ma, C.-H. Xu, F.-F. Ye, X.-G. Xi, M. Li, Y. Lu, and S.-X. Dou, “Pif1 is a force-regulated helicase.” Nucleic Acids Res. 44, 4330-4339 (2016).
  25. H.-P. Ju, Y.-Z. Wang, J. You, X.-M. Hou, X.-G. Xi, S.-X. Dou, W. Li, and P.-Y. Wang, “Folding kinetics of single human telomeric G-quadruplex affected by cisplatin.” ACS Omega 1, 244-250 (2016).
  26. Y.-W. Zhang, D.-G. Nong, S.-X. Dou, W. Li, Y. Yan, X.-G. Xi, C.-H. Xu, and M. Li, “Iterative homology checking and non-uniform stepping during RecA-mediated strand exchange.” Biochem. Biophys. Res. Commun. 478, 1153e1157 (2016).
  27. N.-N. Liu, X.-L. Duan, X. Ai, Y.-T. Yang, M. Li, S.-X. Dou, S. Rety, E. Deprez, and X.-G. Xi, “The Bacteroides sp. 3_1_23 Pif1 protein is a multifunctional helicase.” Nucleic Acids Res. 43, 8942-8954 (2015).
  28. Y. R. Liu, P. Xie, P. Y. Wang, M. Li, H. Li, W. Li, and S.-X. Dou, “A model for chromosome organization during the cell cycle in live E. coli.” Scientific Reports 5, 17133 (2015).
  29. W.-Q. Wu, X.-M. Hou, M. Li, S.-X. Dou, and X.-G. Xi, “BLM unfolds G-quadruplexes in different structural environments through different mechanisms.” Nucleic Acids Res. 43, 4614-4626 (2015).
  30. X.-L. Duan, N.-N. Liu, Y.-T. Yang, H.-H. Li, M. Li, S.-X. Dou, and X.-G. Xi, “G-quadruplexes significantly stimulate Pif1 helicase-catalyzed duplex DNA unwinding.” J. Biol. Chem. 290, 7722-7735 (2015).
  31. S. Wang, W. Qin, J.-H. Li, Y. Lu, K.-Y. Lu, D.-G. Nong, S.-X. Dou, C.-H. Xu, X,-G. Xi, and M. Li, “Unwinding forward and sliding back: an intermittent unwinding mode of the BLM helicase.” Nucleic Acids Res. 43, 3736-3746 (2015).
  32. X.-M. Hou, W.-Q. Wu, X.-L. Duan, N.-N. Liu, H.-H. Li, J. Fu, S.-X. Dou, M. Li, and X.-G. Xi, “Molecular mechanism of G-quadruplex unwinding helicase: sequential and repetitive unfolding of G-quadruplex by Pif1 helicase.” Biochem. J. 466, 189-199 (2015).
  33. H. Li, S.-X. Dou, Y.-R. Liu, W. Li, P. Xie, W.-C. Wang, and P.-Y. Wang. “Mapping intracellular diffusion distribution using single quantum dot tracking: compartmentalized diffusion defined by endoplasmic reticulum.” J. Am. Chem. Soc. 137, 436-444 (2015).
  34. H.-Y. Zhang, L.-Y. Zhang, W.-C. Wang, P. Xie, S.-X. Dou, C. Ji, W. Li, Y.-R. Liu, and P.-Y. Wang. “Oxaliplatin and its enantiomer induce different condensation dynamics of single DNA molecules.” PLOS ONE 8, e71556 (2013).
  35. Y.-R. Liu, S.-X. Dou, C. Ji, W.-C. Wang, P. Xie, H.-Y. Zhang, and P.-Y. Wang. “Transplatin enhances effect of cisplatin on both single DNA molecules and live tumor cells.” Archives Biochem. Biophys. 536, 12-24 (2013).
  36. H. Li, Z. W. Duan, P. Xie, Y. R. Liu, W. C. Wang, S.-X. Dou, and Y. P. Wang. “Effects of paclitaxel on EGFR endocytic trafficking revealed using quantum dot tracking in single cells.” PLOS ONE 7, e45465 (2012).
  37. Y.-N. Xu, N. Bazeille, X.-Y. Ding, X.-M. Lu, P.-Y. Wang, E. Bugnard, V. Grondin, S.-X. Dou, and X. G Xi, “Multimeric BLM is dissociated upon ATP hydrolysis and functions as monomers in resolving DNA structures.” Nucleic Acids Res. 40, 9802-9814 (2012).
  38. W. Li, Z. Q. Sun, P. Xie, S.-X. Dou, W. C. Wang, and P. Y. Wang, “Elastic response and length change of single DNA molecules induced by a combination of cisplatin and transplatin.” Phys. Rev. E 85, 021918 (2012).
  39. S.-X. Dou and X.-G. Xi, “Fluorometric assays for characterizing DNA helicases.” Methods 51, 295-302 (2010).
  40. B.-Y. Pan, S.-X. Dou, Y. Yang, Y.-N. Xu, E. Bugnard, X.-Y. Ding, L. Zhang, P.-Y. Wang, M. Li, and X.-G. Xi, “Mutual inhibition of RecQ molecules in DNA unwinding.” J. Biol. Chem. 285, 15884-15893 (2010).
  41. Y. Yang, S.-X. Dou, Y.-N. Xu, P.-Y. Wang, P. Rigolet, H.-Q. Xu, and X.-G. Xi, “Kinetic mechanism of DNA unwinding by the BLM helicase core and molecular basis for its low processivity.” Biochemistry 49, 656-668 (2010).
  42. X.-M. Hou, X.-H. Zhang, K.-J. Wei, C. Ji, S.-X. Dou, W.-C. Wang, M. Li, P.-Y. Wang, “Cisplatin induces loop structures and condensation of single DNA molecules.” Nucleic Acids Res. 37, 1400-1410 (2009).
  43. B.-Y. Pan, L. Zhang, S.-X. Dou, and P.-Y. Wang, “Forces-induced pinpoint denaturation of short DNA.” Biochem. Biophys. Res. Commun. 388, 137-140 (2009).
  44. X.-M. Hou, W. Li, S.-X. Dou, L.-Y. Zhang, P. Xie, W.-C Wang, P.-Y. Wang, “Formation of DNA toroids inside confined droplets adsorbed on mica surfaces.” Phys. Rev. E 79, 051912 (2009).
  45. B. Sun, K.-J. Wei, B. Zhang, X.-H. Zhang, S.-X. Dou, M. Li, X.-G. Xi, “Impediment of E. coli UvrD by DNA-destabilizing force reveals a strained-inchworm mechanism of DNA unwinding.” EMBO J. 27, 3279-3287 (2008).
  46. Y. Yang, S.-X. Dou, H. Ren, P. Y. Wang, X. D. Zhang, M. Qian, B. Y. Pan, X.-G. Xi, “Evidence for a functional dimeric form of the PcrA helicase in DNA unwinding.” Nucleic Acids Res. 36, 1976-1989 (2008).
  47. H. Ren, S.-X. Dou, X.-D. Zhang, P.-Y. Wang, R. Kanagaraj, J.-L. Liu, P. Janscak, J.-S. Hu, X.-G. Xi, “The zinc-binding motif of human RECQ5β suppresses the intrinsic strand-annealing activity of its DExH helicase domain and is essential for the helicase activity of the enzyme.” Biochem. J. 412, 425-433 (2008).
  48. H. Ren, S.-X. Dou, P. Rigolet, Y. Yang, P.-Y. Wang, M. Amor-Gueret, X.-G. Xi, “The arginine finger of the Bloom syndrome protein: its structural organization and its role in energy coupling.” Nucleic Acids Res. 35, 6029-6041 (2007).
  49. R.-B. Guo, P. Rigolet, H. Ren, B. Zhang, X.-D. Zhang, S.-X. Dou, P.-Y. Wang, M. Amor-Gueret, X.-G. Xi, “Structural and functional analyses of disease-causing missense mutations in Bloom syndrome protein.” Nucleic Acids Res. 35, 6297-6310 (2007).
  50. W. Li, S.-X. Dou, P. Xie, P. Y. Wang, “Brownian dynamics simulation of the effect of histone modification on nucleosome structure.” Phys. Rev. E 75, 051915 (2007).
  51. W. Li, S.-X. Dou, P. Xie, P. Y. Wang, “Browinan dynamics simulation of directional sliding of histone octamers caused by DNA bending.” Phys. Rev. E 73, 051909 (2006).
  52. X. D. Zhang, S.-X. Dou, P. Xie, J. S. Hu, P. Y. Wang, X.-G. Xi, “E. coli RecQ is a rapid, efficient and monomeric helicase.” J. Biol. Chem. 281, 12655-12663 (2006).
  53. X. D. Zhang, S.-X. Dou, P. Xie, P. Y. Wang, X.-G. Xi, “RecQ helicase-catalyzed DNA unwinding detected by fluorescence resonance energy transfer.” Acta Biochimi. Biophys. Sin. 37, 593-600 (2005).
  54. J. L. Liu, P. Rigolet, S.-X. Dou, P. Y. Wang, X.-G. Xi, “The Zinc finger motif of Escherichia coli RecQ is implicated in both DNA binding and protein folding.” J. Biol. Chem. 279, 42794-42802 (2004).
  55. S.-X. Dou, P. Y. Wang, H. Q. Xu, X.-G. Xi, “The DNA binding properties of the Escherichia coli RecQ helicase.” J. Biol. Chem. 279, 6354-6363 (2004).
  56. Y. Y. Liu, P. Y. Wang, S.-X. Dou, W. C. Wang, P. Xie, H. W. Yin, X. D. Zhang, X.-G. Xi, “Ionic effect on combing of single DNA molecules and observation of their force-induced melting by fluorescence microscopy.” J. Chem. Phys. 121, 4302-4309 (2004).