1. 碳一原料如甲醇和二氧化碳等的生物转化利用

2. 微生物如谷氨酸棒杆菌和聚球藻等的基因组编辑

3. 工业菌种优良工业属性的分子机制解析

4. 氨基酸、多元醇等的生物合成

   

085238-生物工程

合成生物学，代谢工程

2012-09--2016-03   上海交通大学   博士
2009-09--2012-06   山东大学   硕士
2005-09--2009-07   山东大学   本科

   

2022-06~现在, 中国科学院天津工业生物技术研究所, 研究员
2018-12~2022-06,中国科学院天津工业生物技术研究所, 副研究员
2016-04~2018-12,中国科学院天津工业生物技术研究所, 助理研究员

   

（1） DaSilva Award, 其他, 2022
（2） 《麻省理工科技评论》亚太区“35岁以下科技创新35人”, 其他, 2022
（3） “伦世仪教育基金”杰出青年学者, 其他, 2022
（4） 天津市优秀科技志愿者, 省级, 2020
（5） 上海交通大学优秀博士学位论文提名论文, 研究所（学校）, 2016
（6） 博士研究生国家奖学金, 国家级, 2015
（7） 上海市明治生命科学奖, 市地级, 2014
（8） 博士研究生国家奖学金, 国家级, 2014
（9） 挑战杯中国大学生创业计划竞赛金奖, 国家级, 2010

（ 1 ） 具有天冬氨酸激酶活性的多肽及其在生产氨基酸中的应用, 发明专利, 2021, 第 3 作者, 专利号: CN113201514A

（ 2 ） 一种提高微生物对甲醇的耐受性和利用率的方法, 发明专利, 2021, 第 2 作者, 专利号: CN112680389A

（ 3 ） 一种提高菌株的甲醇生物耐受性和生物转化效率的方法, 发明专利, 2021, 第 2 作者, 专利号: CN112680468A

（ 4 ） γ-谷氨酰激酶突变体及其应用, 专利授权, 2021, 第 3 作者, 专利号: CN112111469B

（ 5 ） 赖氨酸生产菌株的构建方法和应用, 专利授权, 2021, 第 7 作者, 专利号: CN110129247B

（ 6 ） 一种赖氨酸生产菌株的构建方法和应用, 发明专利, 2021, 第 7 作者, 专利号: CN112251391A

（ 7 ） 5-氨基乙酰丙酸生产菌株及其构建方法和应用, 发明专利, 2020, 第 5 作者, 专利号: CN110862952A

（ 8 ） 甲醇生物转化菌株的构建方法以及构建的菌株和应用, 发明专利, 2020, 第 2 作者, 专利号: CN110760536A

（ 9 ） 一种菌及其构建方法和应用, 发明专利, 2016, 第 2 作者, 专利号: CN106190936A

（ 10 ） 一种菌及其获取方法和应用, 发明专利, 2016, 第 4 作者, 专利号: CN106190901A

（ 11 ） 一种利用基因工程蓝藻生产三碳化合物的方法, 发明专利, 2015, 第 2 作者, 专利号: CN104726505A

   

[1] Pu, Wei, Chen, Jiuzhou, Zhou, Yingyu, Qiu, Huamin, Shi, Tuo, Zhou, Wenjuan, Guo, Xuan, Cai, Ningyun, Tan, Zijian, Liu, Jiao, Feng, Jinhui, Wang, Yu, Zheng, Ping, Sun, Jibin. Systems metabolic engineering of Escherichia coli for hyper-production of 5-aminolevulinic acid. BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS[J]. 2023, 16(1): http://dx.doi.org/10.1186/s13068-023-02280-9.
[2] Pu, Wei, Chen, Jiuzhou, Liu, Pi, Shen, Jie, Cai, Ningyun, Liu, Baoyan, Lei, Yu, Wang, Lixian, Ni, Xiaomeng, Zhang, Jie, Liu, Jiao, Zhou, Yingyu, Zhou, Wenjuan, Ma, Hongwu, Wang, Yu, Zheng, Ping, Sun, Jibin. Directed evolution of linker helix as an efficient strategy for engineering LysR-type transcriptional regulators as whole-cell biosensors. BIOSENSORS & BIOELECTRONICS[J]. 2023, 222: http://dx.doi.org/10.1016/j.bios.2022.115004.
[3] Bo Xin, Cheng Zhong, Yu Wang. Integrating the marine carbon resource mannitol into biomanufacturing. TRENDS IN BIOTECHNOLOGY[J]. 2023, 41(6): 745-749, http://dx.doi.org/10.1016/j.tibtech.2022.12.010.
[4] 卢挥, 张启, 于思礼, 王钰, 康明, 韩双艳, 刘叶, 王猛. 谷氨酸棒杆菌中基于CRISPR/Cas9的多位点碱基编辑系统的优化. 生物工程学报[J]. 2022, 38(2): 780-795, http://lib.cqvip.com/Qikan/Article/Detail?id=7106678265.
[5] Jiuzhou Chen, Yu Wang, Ping Zheng, jibin sun. Engineering synthetic auxotrophs for growth-coupled directed protein evolution. Trends in Biotechnology[J]. 2022, [6] Jiao Liu, Moshi Liu, Tuo Shi, Guannan Sun, Ning Gao, Xiaojia Zhao, Xuan Guo, Xiaomeng Ni, Qianqian Yuan, Jinhui Feng, Zhemin Liu, Yanmei Guo, Jiuzhou Chen, Yu Wang, Ping Zheng, Jibin Sun. CRISPR-assisted rational flux-tuning and arrayed CRISPRi screening of an l -proline exporter for l -proline hyperproduction. NATURE COMMUNICATIONS[J]. 2022, 13: [7] Wang, Yu, Zhao, Dongdong, Sun, Letian, Wang, Jie, Fan, Liwen, Cheng, Guimin, Zhang, Zhihui, Ni, Xiaomeng, Feng, Jinhui, Wang, Meng, Zheng, Ping, Bi, Changhao, Zhang, Xueli, Sun, Jibin. Engineering of the Translesion DNA Synthesis Pathway Enables Controllable C-to-G and C-to-A Base Editing in Corynebacterium glutamicum. ACS SYNTHETIC BIOLOGY[J]. 2022, 11(10): 3368-3378, http://dx.doi.org/10.1021/acssynbio.2c00265.
[8] Sun, Xue, Li, Qinggang, Wang, Yu, Zhou, Wenjuan, Guo, Yanmei, Chen, Jiuzhou, Zheng, Ping, Sun, Jibin, Ma, Yanhe. Isoleucyl-tRNA synthetase mutant based whole-cell biosensor for high-throughput selection of isoleucine overproducers. BIOSENSORS & BIOELECTRONICS[J]. 2021, 172: http://dx.doi.org/10.1016/j.bios.2020.112783.
[9] 凡立稳, 王钰, 郑平, 孙际宾. 一碳代谢关键酶——甲醇脱氢酶的研究进展与展望. 生物工程学报[J]. 2021, 37(2): 530-540, http://lib.cqvip.com/Qikan/Article/Detail?id=7104000438.
[10] Yu Wang, Haijiao Cheng, Yang Liu, Ye Liu, Xiao Wen, Kun Zhang, Xiaomeng Ni, Ning Gao, Liwen Fan, Zhihui Zhang, Jiao Liu, Jiuzhou Chen, Lixian Wang, Yanmei Guo, Ping Zheng, Meng Wang, Jibin Sun, Yanhe Ma. In-situ generation of large numbers of genetic combinations for metabolic reprogramming via CRISPR-guided base editing. NATURE COMMUNICATIONS[J]. 2021, 12(1): http://dx.doi.org/10.1038/s41467-021-21003-y.
[11] Zhang, Zhihui, Wang, Yu, Zheng, Ping, Sun, Jibin. Promoting Lignin Valorization by Coping with Toxic C1 Byproducts. TRENDS IN BIOTECHNOLOGY. 2021, 39(4): 331-335, http://dx.doi.org/10.1016/j.tibtech.2020.09.005.
[12] Wang, Yu, Liu, Ye, Zheng, Ping, Sun, Jibin, Wang, Meng. Microbial Base Editing: A Powerful Emerging Technology for Microbial Genome Engineering. TRENDS IN BIOTECHNOLOGYnull. 2021, 39(2): 165-180, http://dx.doi.org/10.1016/j.tibtech.2020.06.010.
[13] 陈久洲, 王钰, 蒲伟, 郑平, 孙际宾. 5-氨基乙酰丙酸生物合成技术的发展及展望. 合成生物学[J]. 2021, 2(6): 1000-1016, http://lib.cqvip.com/Qikan/Article/Detail?id=7106434784.
[14] Li, Mingyue, Chen, Jiuzhou, Wang, Yu, Liu, Jiao, Huang, Jingwen, Chen, Ning, Zheng, Ping, Sun, Jibin. Efficient Multiplex Gene Repression by CRISPR-dCpf1 in Corynebacterium glutamicum. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY[J]. 2020, 8: https://doaj.org/article/f87f64c6d0d9420fbbd0fbdea99eda61.
[15] Yu, Sili, Price, Marcus A, Wang, Yu, Liu, Yang, Guo, Yanmei, Ni, Xiaomeng, Rosser, Susan J, Bi, Changhao, Wang, Meng. CRISPR-dCas9 Mediated Cytosine Deaminase Base Editing in Bacillus subtilis. ACS SYNTHETIC BIOLOGY[J]. 2020, 9(7): 1781-1789, https://www.webofscience.com/wos/woscc/full-record/WOS:000551555500027.
[16] Chen, Jiuzhou, Wang, Yu, Guo, Xuan, Rao, Deming, Zhou, Wenjuan, Zheng, Ping, Sun, Jibin, Ma, Yanhe. Efficient bioproduction of 5-aminolevulinic acid, a promising biostimulant and nutrient, from renewable bioresources by engineered Corynebacterium glutamicum. BIOTECHNOLOGY FOR BIOFUELS[J]. 2020, 13(1): https://doaj.org/article/76327970ce5a4277b911a00b0b226b6a.
[17] Wang, Yu, Fan, Liwen, Tuyishime, Philibert, Liu, Jiao, Zhang, Kun, Gao, Ning, Zhang, Zhihui, Ni, Xiaomeng, Feng, Jinhui, Yuan, Qianqian, Ma, Hongwu, Zheng, Ping, Sun, Jibin, Ma, Yanhe. Adaptive laboratory evolution enhances methanol tolerance and conversion in engineered Corynebacterium glutamicum. COMMUNICATIONS BIOLOGY[J]. 2020, 3(1): http://dx.doi.org/10.1038/s42003-020-0954-9.
[18] 李俊维, 刘叶, 王钰, 郁彭, 郑平, 王猛. 谷氨酸棒杆菌碱基编辑的条件优化. 生物工程学报[J]. 2020, 36(1): 143-151, http://lib.cqvip.com/Qikan/Article/Detail?id=7100862753.
[19] Wang, Yu, Fan, Liwen, Tuyishirne, Philibert, Zheng, Ping, Sun, Jibin. Synthetic Methylotrophy: A Practical Solution for Methanol-Based Biomanufacturing. TRENDS IN BIOTECHNOLOGY[J]. 2020, 38(6): 650-666, http://dx.doi.org/10.1016/j.tibtech.2019.12.013.
[20] Zhang, Kun, Zhang, Zhihui, Kang, Jianan, Chen, Jiuzhou, Liu, Jiao, Gao, Ning, Fan, Liwen, Zheng, Ping, Wang, Yu, Sun, Jibin. CRISPR/Cas13d-Mediated Microbial RNA Knockdown. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY[J]. 2020, 8: https://doaj.org/article/d85c16031ef74534b0f4a19a090235cc.
[21] Sun, Dehu, Chen, Jiuzhou, Wang, Yu, Li, Mingyue, Rao, Deming, Guo, Yanmei, Chen, Ning, Zheng, Ping, Sun, Jibin, Ma, Yanhe. Metabolic engineering of Corynebacterium glutamicum by synthetic small regulatory RNAs. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY[J]. 2019, 46(2): 203-208, https://www.webofscience.com/wos/woscc/full-record/WOS:000457989900007.
[22] Wang, Yu, Liu, Ye, Li, Junwei, Yang, Yi, Ni, Xiaomeng, Cheng, Haijiao, Huang, Teng, Guo, Yanmei, Ma, Hongwu, Zheng, Ping, Wang, Meng, Sun, Jibin, Ma, Yanhe. Expanding targeting scope, editing window, and base transition capability of base editing in Corynebacterium glutamicum. BIOTECHNOLOGY AND BIOENGINEERING[J]. 2019, 116(11): 3016-3029, [23] Zhu, Chengchao, Chen, Jiuzhou, Wang, Yu, Wang, Lixian, Guo, Xuan, Chen, Ning, Zheng, Ping, Sun, Jibin, Ma, Yanhe. Enhancing 5-aminolevulinic acid tolerance and production by engineering the antioxidant defense system of Escherichia coli. BIOTECHNOLOGY AND BIOENGINEERING[J]. 2019, 116(8): 2018-2028, [24] Fan, Liwen, Wang, Yu, Tuyishime, Philibert, Gao, Ning, Li, Qinggang, Zheng, Ping, Sun, Jibin, Ma, Yanhe. Engineering Artificial Fusion Proteins for Enhanced Methanol Bioconversion. CHEMBIOCHEM[J]. 2018, 19(23): 2465-2471, https://www.webofscience.com/wos/woscc/full-record/WOS:000452035700008.
[25] Wang, Yu, Cao, Guoqiang, Xu, Deyu, Fan, Liwen, Wu, Xinyang, Ni, Xiaomeng, Zhao, Shuxin, Zheng, Ping, Sun, Jibin, Ma, Yanhe. A Novel Corynebacterium glutamicum L-Glutamate Exporter. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2018, 84(6): https://www.webofscience.com/wos/woscc/full-record/WOS:000426389400027.
[26] Liu, Jiao, Wang, Yu, Zheng, Ping, Sun, Jibin. CRISPR/Cas9-mediated ssDNA Recombineering in Corynebacterium glutamicum. BIO-PROTOCOL[J]. 2018, 8(19): http://dx.doi.org/10.21769/BioProtoc.3038.
[27] Wang, Yu, Liu, Ye, Liu, Jiao, Guo, Yanmei, Fan, Liwen, Ni, Xiaomeng, Zheng, Xiaomei, Wang, Meng, Zheng, Ping, Sun, Jibin, Ma, Yanhe. MACBETH: Multiplex automated Corynebacterium glutamicum base editing method. METABOLIC ENGINEERING[J]. 2018, 47: 200-210, http://dx.doi.org/10.1016/j.ymben.2018.02.016.
[28] Liu, Jiao, Wang, Yu, Lu, Yujiao, Ni, Xiaomeng, Guo, Xuan, Zhao, Jing, Chen, Jiuzhou, DeleOsibanjo, Taiwo, Zheng, Ping, Sun, Jibin, Ma, Yanhe. Mutations in Peptidoglycan Synthesis Gene ponA Improve Electrotransformation Efficiency of Corynebacterium glutamicum ATCC 13869. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2018, 84(24): https://www.webofscience.com/wos/woscc/full-record/WOS:000451988000019.
[29] Tuyishime, Philibert, Wang, Yu, Fan, Liwen, Zhang, Qiongqiong, Li, Qinggang, Zheng, Ping, Sun, Jibin, Ma, Yanhe. Engineering Corynebacterium glutamicum for methanol-dependent growth and glutamate production. METABOLIC ENGINEERING[J]. 2018, 49: 220-231, http://dx.doi.org/10.1016/j.ymben.2018.07.011.
[30] Zhang, Qiongqiong, Zheng, Xiaomei, Wang, Yu, Yu, Jiandong, Zhang, Zhidan, DeleOsibanjo, Taiwo, Zheng, Ping, Sun, Jibin, Jia, Shiru, Ma, Yanhe. Comprehensive optimization of the metabolomic methodology for metabolite profiling of Corynebacterium glutamicum. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2018, 102(16): 7113-7121, https://www.webofscience.com/wos/woscc/full-record/WOS:000440109200029.
[31] Xiaolu Wang, Yu Wang, Jiao Liu, Qinggang Li, Zhidan Zhang, Ping Zheng, Fuping Lu, Jibin Sun. Biological conversion of methanol by evolved Escherichia coli carrying a linear methanol assimilation pathway. BIORESOURCES AND BIOPROCESSING[J]. 2017, 4(1): 1-6, http://dx.doi.org/10.1186/s40643-017-0172-6.
[32] Wang, Yu, Tao, Fei, Xin, Bo, Liu, Hongyu, Gao, Yanyan, Zhou, NingYi, Xu, Ping. Switch of metabolic status: redirecting metabolic flux for acetoin production from glycerol by activating a silent glycerol catabolism pathway. METABOLIC ENGINEERING[J]. 2017, 39: 90-101, http://dx.doi.org/10.1016/j.ymben.2016.10.020.
[33] Liu, Jiao, Wang, Yu, Lu, Yujiao, Zheng, Ping, Sun, Jibin, Ma, Yanhe. Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium glutamicum. MICROBIAL CELL FACTORIES[J]. 2017, 16(1): http://dx.doi.org/10.1186/s12934-017-0815-5.
[34] 王晓璐, 王钰, 刘娇, 郑平, 路福平. 利用定向进化提高基因工程大肠杆菌的甲醇利用能力. 生物技术通报[J]. 2017, 33(9): 101-109, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=6073830&detailType=1.
[35] Wang, Yu, Tao, Fei, Ni, Jun, Li, Chao, Xu, Ping. Production of C3 platform chemicals from CO2 by genetically engineered cyanobacteria. GREEN CHEMISTRY[J]. 2015, 17(5): 3100-3110, https://www.webofscience.com/wos/woscc/full-record/WOS:000354409500058.
[36] Wang, Yu, Tao, Fei, Tang, Hongzhi, Xu, Ping. Genome Sequence of Clostridium diolis Strain DSM 15410, a Promising Natural Producer of 1,3-Propanediol. MICROBIOLOGY RESOURCE ANNOUNCEMENTS[J]. 2013, 1(4): [37] Wang Yu, Tao Fei, Xu Ping. Glycerol Dehydrogenase Plays a Dual Role in Glycerol Metabolism and 2,3-Butanediol Formation in Klebsiella pneumoniae *. THE JOURNAL OF BIOLOGICAL CHEMISTRY. 289(9): 6080-6090, http://dx.doi.org/10.1074/jbc.M113.525535.
[38] Wang Yu, Tao Fei, Li Chao, Li Lixiang, Xu Ping. Genome Sequence of Klebsiella pneumoniae Strain ATCC 25955, an Oxygen-Insensitive Producer of 1,3-Propanediol. GENOME ANNOUNCEMENTS. 1(4): 

   

（ 1 ） 谷氨酸棒杆菌的甲醇利用限制因素研究, 负责人, 国家任务, 2018-01--2020-12
（ 2 ） 二氧化碳的人工生物转化, 参与, 中国科学院计划, 2016-01--2018-12
（ 3 ） 面向天然产物生物合成的人工细胞, 参与, 中国科学院计划, 2018-01--2022-12
（ 4 ） UriR/RbsR参与谷氨酸棒杆菌抵御高温压力的机制研究, 参与, 国家任务, 2019-01--2022-12
（ 5 ） 高版本模式微生物底盘细胞, 参与, 国家任务, 2019-07--2024-06
（ 6 ） 超进化聚球藻底盘细胞的设计构建, 参与, 国家任务, 2019-07--2024-06
（ 7 ） 甲醇为唯一碳源的谷氨酸棒杆菌构建及机制解析, 负责人, 国家任务, 2021-01--2024-12
（ 8 ） 基金委优秀青年基金—微生物基因编辑育种, 负责人, 国家任务, 2023-01--2025-12

（1）Genome Engineering of Corynebacterium glutamicum for C1-based Biomanufacturing   2019-01-13
（2）Learn from the industrial Corynebacterium glutamicum strains   2018年天津科大-天津工业生物所中青年学者学术论坛   2018-11-06
（3）工业平台菌株C1底物谱拓展及应用   2018年中国微生物学会学术年会   2018-10-21
（4）非糖质原料的生物转化与化学品合成   中国生物工程学会第三届青年科技论坛   2018-04-15

   

学生姓名		培养单位	学位类别	学科专业	入学/毕业时间
Philibert Tuyishime		中国科学院天津工业生物技术研究所	博士	071010	2017-09-01/ 2019-06-30
高宁		中国科学院天津工业生物技术研究所	专业硕士	085238	2018-09-01/ 2021-06-30
张昆		中国科学院天津工业生物技术研究所	专业硕士	085238	2017-09-01/ 2020-06-30
凡立稳		中国科技大学	博士	071010	2016-09-01/ 2021-06-30
王晓璐		天津科技大学	专业硕士	085238	2015-09-01/ 2017-06-30