基本信息
鄢慧民 男 博导 武汉病毒研究所
电子邮件:hmyan@wh.iov.cn
通信地址:湖北省武汉市武昌小洪山中区44号
邮政编码:430071

粘膜免疫

粘膜免疫抗病毒机制及其应用。研究内容主要以麻疹病毒、艾滋病病毒、SARS冠状病毒等为研究对象,研究IgA抗体在粘膜免疫防御中的抗病毒功能及其应用,包括IgA单克隆抗体和粘膜疫苗的抗病毒免疫预防和治疗;基于细菌鞭毛素蛋白的粘膜佐剂免疫识别机制和应用;粘膜佐剂和粘膜疫苗及其与粘膜上皮细胞的相互作用及粘膜免疫效应。

招生信息

   
招生专业
071005-微生物学
071010-生物化学与分子生物学
100102-免疫学
招生方向
粘膜免疫
病毒感染
疫苗

教育背景

   
学历
1982年9月至1986年6月:武汉大学生物系遗传学专业,理学学士学位
1995年9月至1998年9月:武汉大学生命科学学院遗传学,理学博士学位
1998年9月至2002年9月:美国俄亥俄州克里夫兰市凯斯西保留地大学医学院病理学系(Case Western Reserve University,Cleveland, Ohio, USA)博士后

工作经历

   
工作简历
2006-07--今 中国科学院武汉病毒研究所 研究员
1998-07--2002-08 Case Western Reserve University,School of Medicine Research associate
1986-06--2006-06 武汉大学 助教,讲师,副教授,博导

教授课程

粘膜免疫学

专利与奖励

   
专利成果
(1) 鄢慧民,刘芳,杨菁毅。一种改造的鞭毛素蛋白及其制备方法和应用,发明,2009,第1作者,专利号:ZL 200910194284.1
(2) ZL 201110438088.1. VACCINE FOR DENTAL CARIES AND METHODS FOR PREPARING THE SAME.(龋齿疫苗及制备方法。)China (中国)Huimin YAN, Wei SHI, Ying SUN, Jingyi YANG。(鄢慧民,石伟,孙颖,杨菁毅)。,发明,2014,第1作者,专利号:ZL 201110438088.1.
(3) US 8,449,891 B2. Recombinant Flagellin Protein and Preparation and Use Thereof. Huimin YAN, Fang LIU, Jingyi YANG. Date of Patent: May 28, 2013,发明,2013,第1作者,专利号:US 8,449,891 B2.

出版信息

   
发表论文
(1) Rong Bao, Jingyi Yang, Ying Sun, Dihan Zhou, Yi Yang, Yaoming Li, Yuan Cao, Yang Xiao, Wei Li, Jie Yu, Bali Zhao, Maohua Zhong, Huimin Yan. Flagellin-PAc Fusion Protein Inhibits Progression of Established Caries. J Dent Res. 2015; first published on April 16, 2015 as doi:10.1177/0022034515582224,J Dent Res.,2015,通讯作者
(2) Wei Li, Jingyi Yang, Ejuan Zhang, Maohua Zhong, Yang Xiao, Jie Yu, Dihan Zhou, Yuan Cao, Yi Yang, Yaoming Li and Huimin Yan. Activation of NLRC4 downregulates TLR5-mediated antibody immune responses against flagellin. Cell Mol Immunol. advance online publication, April 27, 2015; doi:10.1038/cmi.2015.033,Cell Mol Immunol,2015,通讯作者
(3) Jingchang Xue, Yaoming Li*, Xiaoyi Xu, Jie Yu, Hu Yan, Huimin Yan. EV71 infection correlates with viral IgG preexisting at pharyngolaryngeal mucosa in children. Virologica Sinica, 2015. 30 (2): 1-7.,Virologica Sinica,2015,第5作者
(4) Yang Xiao, Fang Liu, Jingyi Yang, Maohua Zhong, Ejuan Zhang, Yaoming Li, Dihan Zhou, Yuan Cao, Wei Li, Jie Yu, Yi Yang and Huimin Yan. Over-activation of TLR5 signaling by high-dose flagellin induces liver injury in mice. Cell Mol Immunol. 2015. (in press) doi:10.1038/cmi.2014.110,Cell Mol Immunol,2015,通讯作者
(5) Jingyi Yang, Ejuan Zhang, Fang Liu, Yan Zhang, Maohua Zhong, Yaoming Li, Dihan Zhou, Yaoqing Chen, Yuan Cao, Yang Xiao, Benxia He, Yi Yang, Ying Sun, Mengji Lu, Huimin Yan. Flagellins of Salmonella Typhi and non-pathogenic Escherichia coli are differentially recognized through NLRC4 pathway in macrophages. Journal of Innate Immunity. 2014. 6:47-57 (DOI: 10.1159/000351476),Journal of Innate Immunity,2014,通讯作者
(6) Diana Kozlova, Viktoriya Sokolova, Maohua Zhong, Ejuan Zhang, Jingyi Yang, Wei Li, Yi Yang, Jan Buer, Astrid Maria Westendorf, Matthias Epple, Huimin Yan. Calcium phosphate nanoparticles show an effective activation of the innate immune response in vitro and in vivo after functionalization with flagellin. Virologica Sinica. 2014. 29(1): 33-39,Virologica Sinica,2014,通讯作者
(7) Huimin Yan. Salivary IgA enhancement strategy for development of a nasal-spray anti-caries mucosal vaccine. Sci China Life Sci. 2013. 56(5):406-413.,Sci China Life Sci.,2013,通讯作者
(8) Jingyi Yang, Maohua Zhong, Yan Zhang, Ejuan Zhang, Ying Sun, Yuan Cao, Yaoming Li, Dihan Zhou, Benxia He, Yaoqing Chen, Yi Yang, Jie Yu, Huimin Yan. Antigen replacement of domains D2 and D3 in flagellin promotes mucosal IgA production and attenuates flagellin-induced inflammatory response after intranasal immunization. Human Vaccines Immunotherapeutics, 2013. 9(5): 1084-1092.,Human Vaccines Immunotherapeutics,2013,通讯作者
(9) Yaoqing Chen, Luyang Cao, Maohua Zhong, Yan Zhang, Chen Han, Qiaoli Li, Jingyi Yang, Dihan Zhou, Wei Shi, Benxia He, Fang Liu, Jie Yu, Ying Sun, Yuan Cao, Yaoming Li, Wenxin Li, Deying Guo, Zhijian Cao, Huimin Yan. Anti-HIV-1 Activity of a New Scorpion Venom Peptide Derivative Kn2-7. PLoS ONE. 2012. 7(4): e34947. , PLoS ONE,2012,通讯作者
(10) Maohua Zhong, Benxia He, Jingyi Yang, Rong Bao, Yan Zhang, Dihan Zhou, Yaoqing Chen, Liangzhu Li, Chen Han, Yi Yang, Ying Sun, Yuan Cao, Yaoming Li, Wei Shi, Shibo Jiang, Xiaoyan Zhang, Huimin Yan. L-Selectin and P-Selectin Are Novel Biomarkers of Cervicovaginal Inflammation for Preclinical Mucosal Safety Assessment of Anti-HIV-1 Microbicide. Antimicrob Agents Chemother, 2012. 56(6):3121-3132mmation for Preclinical Mucosal Safety Assessment of Anti-HIV-1 Microbicide,Antimicrobial Agents and Chemotherapy,2012,通讯作者
(11) Yaoming Li, Fang Liu, Chen Han, Huimin Yan. Monoclonal antibody that blocks the toll-like receptor 5 binding region of flagellin. Hybridoma (Larchmt). 2012. Feb;31(1):60-62.,Hybridoma,2012,通讯作者
(12) Ying Sun, Wei Shi , Jingyi Yang, Dihan Zhou, Yaoqing Chen, Yan Zhang, Yi Yang, Benxia He, Maohua Zhong, Yaoming Li, Yuan Cao, Yang Xiao, Wei Li, Jie Yu, Yuhong Li, Mingwen Fan, Huimin Yan. Flagellin-PAc Fusion Protein Is a High Efficacy Anti-caries Mucosal Vaccine. J Dent Res. 2012. 91(10): 941-947,J Dent Res.,2012,通讯作者
(13) Wei Shi, Yuhong Li, Fang Liu, Jingyi Yang, Dihan Zhou, Yaoqing Chen,Yan Zhang, Yi Yang, Benxia He, Chen Han, Mingwen Fan, Huimin Yan. Flagellin enhances saliva IgA response and protection of anti-caries DNA vaccine. J Dent Res. 2012. 91(3):249-54 , J Dent Res. ,2012,通讯作者
(14) Weiwei Gai, Yan Zhang, Dihan Zhou, Yaoqing Chen,Jingyi Yang, Huimin Yan. PIKA Provides an Adjuvant Effect to Induce Strong Mucosal and Systemic Humoral Immunity Against SARS-CoV. Virologica Sinica, 2011. 26(2):81-94.mic Humoral Immunity Against SARS-CoV,Virologica Sinica ,2011,通讯作者
(15) Qiaoli Li, Zhenhuan Zhao, Dihan Zhou, Yaoqing Chen, Wei Hong, Luyang Cao, Jingyi Yang, Yan Zhang, Wei Shi, Zhijian Cao, Yingliang Wu, Huimin Yan, Wenxin Li. Virucidal activity of a scorpion venom peptide variant mucroporin-M1 against measles, SARS-CoV and influenza H5N1 viruses. Peptides. 2011. 32:(7),1518-1525.,Peptides,2011,通讯作者
(16) Yan Zhang, Jingyi Yang, Rong Bao, Yaoqing Chen, Dihan Zhou, Benxia He, Maohua Zhong, Yaoming Li, Fang Liu, Qiaoli Li, Yi Yang, Chen Han, Ying Sun, Yuan Cao, Huimin Yan. Unpolarized Release of Vaccinia Virus and HIV Antigen by Colchicine Treatment Enhances Intranasal HIV Antigen Expression and Mucosal Humoral Responses. PLoS ONE. 2011. (9): e24296. doi:10.1371/journal.pone.0024296,PLoS ONE,2011,通讯作者
(17) Dihan Zhou, Yan Zhang, Qiaoli Li, Yaoqing Chen, Benxia He, Jingyi Yang, Haobo Tu, Lei Lei, and Huimin Yan. Matrix protein-specific IgA antibody inhibits measles virus replication by intracellular neutralization. J. Virol. 2011. 85(21):11090-7. by intracellular neutralization.,Journal of Virology,2011,通讯作者
(18) Fang Liu, Jingyi Yang, Yan Zhang, Dihan Zhou, Yaoqing Chen, Weiwei Gai, Wei Shi, Qiaoli Li, Po Tien and Huimin Yan. Recombinant flagellins with partial deletions of the hypervariable domain lose antigenicity but not mucosal adjuvancy. Biochemical and Biophysical Research Communications, 2010. 392:582-587.,Biochemical and Biophysical Research Communications,2010,通讯作者

科研活动

   
科研项目
(1) 持续性病毒感染中病毒与免疫细胞,参与,国家级,2015-01--2017-12
(2) 能克服粘膜上皮细胞极性屏障的重组痘苗病毒HIV 粘膜疫苗载体的构建,主持,国家级,2013-05--2016-05
(3) “预防性艾滋病疫苗研究”项目子课题“鞭毛素佐剂研究”,主持,国家级,2012-05--2015-05
(4) 粘膜免疫技术研究,主持,国家级,2008-09--2011-04
(5) 预防龋病DNA疫苗的临床前研究,参与,国家级,2008-05--2011-03
(6) 痘苗病毒载体重组AIDS疫苗与粘膜上皮细胞的相互作用,主持,国家级,2007-01--2009-12

指导学生

已指导学生

刘芳  博士研究生  071005-微生物学  

石伟  硕士研究生  071010-生物化学与分子生物学  

李乔丽  硕士研究生  071010-生物化学与分子生物学  

张燕  博士研究生  071010-生物化学与分子生物学  

韩陈  硕士研究生  071010-生物化学与分子生物学  

何本霞  硕士研究生  071010-生物化学与分子生物学  

周谛晗  博士研究生  071010-生物化学与分子生物学  

现指导学生

杨菁毅  博士研究生  071010-生物化学与分子生物学  

操媛  硕士研究生  071010-生物化学与分子生物学  

孙颖  博士研究生  071010-生物化学与分子生物学  

包容  博士研究生  071010-生物化学与分子生物学  

俞杰  硕士研究生  071010-生物化学与分子生物学  

肖杨  博士研究生  071010-生物化学与分子生物学  

李伟  博士研究生  071010-生物化学与分子生物学