Diagnostic Microbiology;

Bactericidal lysins and Bacteriophage therapy

1996. 6 – 1999. 6, PhD, Department of Chemistry, National University of Singapore, Singapore；

1988. 9 – 1992. 6, BSc, Department of Chemistry, Wuhan University, China.

2008. 1 - now, Principle Investigator of Diagnostic Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, China

2005. 5 - 2007. 12, Professor, Wuhan Institute of Virology, Chinese Academy of Sciences, China

2004.9 – 2005. 3, Professor, College of Life Sciences and Technologies, Huazhong University of Sciences and Technologies, China

1999.7 – 2004. 8, Senior Research Scientist，CE Resources Pte Ltd, Singapore

1992.7 – 1996. 5, Research Assistant, Wuhan Institute of Virology, Chinese Academy of Sciences, China

2004.10-2005.3 Professor, Colleage of Life Sciences and Technologies, Huazhong University of Sciences and Technologies.

1.  Jun Luo, Junhua Li, Hang Yang, Junping Yu, Hongping Wei*, Accurate Detection of MethicillinResistant Staphylococcus aureus in Mixtures by Use of Single-Bacterium Duplex Droplet Digital PCR, Journal of Clinical Microbiology, 2017, 55, 2946-2955.
2. Hang Yang, Huaidong Zhang, Jing Wang, Junping Yu, Hongping Wei*, A novel chimeric lysin with robust antibacterial activity against planktonic and biofilm methicillinresistant Staphylococcus aureus, Scientific RepoRts, 7:40182, DOI: 10.1038/srep40182.
3. Chenghui Yan, Yun Zhang, Hang Yang, Junping Yu*, Hongping Wei*, Combining phagomagnetic separation with immunoassay for specific, fast and sensitive detection of Staphylococcus aureus, Talanta, 2017, 170, 291-297.
4. Junping Yu, Yun Zhang, Yun Zhang, Huang Li, Hang Yang, Hongping Wei*. Sensitive and rapid detection of staphylococcus aureus in milk via cell binding domain of lysin, Biosens Bioelectron, 2016 (1), 366-371.
5. Yun Zhang，Hang Yang，Junping Yu*，Hongping Wei*，Rapid and sensitive detection of HIV-1 p24 antigen by immunomagnetic separation coupled with catalytic fluorescent immunoassay, Anal Bio anal Chem, 2016, 408:6115–6121.
6. Yuanyuan Hu, Hang Yang, Jing Wang, Yun Zhang, Junping Yu, Hongping Wei*. Comparison between a chimeric lysin ClyH and other enzymes for extracting DNA to detect methicillin resistant Staphylococcus aureus by quantitative PCR, World J Microbiol Biotechnol, 2016 , 32 (1), doi: 10.1007/s11274-015-1971-6.
7. Heng Li, Liping Zhou, Jun Luo, Junping Yu, Hang Yang, Hongping Wei*, Rapid colorimetric pyrazinamide susceptibility testing of Mycobacterium tuberculosis, INT J TUBERC LUNG DIS, 2016, 20(4), 462-467.
8. Hang Yang, Sara B. Linden, Jing Wang, Junping Yu, Daniel C. Nelson*, Hongping Wei*, A chimeolysin with extended-spectrum streptococcal host range found by an induced lysis-based rapid screening method, Scientific Report, 2015, 26;5:17257. doi: 10.1038/srep17257.
9. Hang Yang, Mengyue Wan, Junping Yu, Hongping Wei*, Antibacterial Activity of a Novel Peptide-Modified Lysin Against Acinetobacter baumannii and Pseudomonas aeruginosa, Front Microbiol., 2015, 22;6:1471. doi: 10.3389/fmicb.2015.01471.
10. Wei Hu, Yun Zhang, Hang Yang, Junping Yu, Hongping Wei*. Simple and rapid preparation of red fluorescence and red color S. aureus derived nanobioparticles for pathogen detection, J Microbiol Methods 2015, 115:47-53.
11. Hang Yang, Mengyue Wang, Junping Yu, Hongping Wei*，Aspartate inhibits Staphylococcus aureus biofilm formation，FEMS Microbiol Lett. 2015, DOI: http://dx.doi.org/10.1093/femsle/fnv025.
12. Hongping Wei. Bacteriophages, revitalized after 100 years in the shadow of antibiotics, Virologica Sinica, 2015,30(1): 1-2.
13. Yanling Huang, Hang Yang, Junping Yu, Hongping Wei*, Molecular dissection of phage lysin PlySs2: integrity of the catalytic and cell wall binding domains is essential for its broad lytic activity. Virol Sin. 2015 Feb; 30(1):45-51.
14. TieshanTeng, Junping Yu, Hang Yang, Hongping Wei*. Isolation and complete genome sequence of a novel virulent mycobacteriophage, CASbig. Virol Sin. 2015 Feb;30(1):76-9.
15. TieshanTeng, Jiafa Liu, Hongping Wei*. Anti-mycobacterial peptides: from human to phage. Cell Physiol Biochem. 2015;35(2):452-66. Review
16. Qiuhua Dong, Jing Wang, Hang Yang, Cuihua Wei, Junping Yu, Yun Zhang, Yanling Huang, Xian-En Zhang*, Hongping Wei*. Construction of a chimeric lysin Ply187N-V12C with extended lytic activity against staphylococci and streptococci. Microb Biotechnol. 2015 (8), 210-220.
17. Hang Yang, Junping Yu, Hongping Wei*. Engineered bacteriophage lysins as novel anti-infectives. Front Microbiol. vol. 5, 2014, pp. 542.
18. Li Huang, Xiumei Hu, Man Zhou, Yinmei Yang, Jinjuan Qiao, Dianbing Wang, Junping Yu, Zongqiang Cui, Zhiping Zhang, Xian-En Zhang, Hongping Wei*. Rapid Detection of New Delhi Metallo-β-Lactamase Gene and Variants coding Carbapenemase with Different Activity by a PCR-based in vitro protein expression method. Journal of Clinical Microbiology, vol.52, 2014, pp. 1947-1953.
19. Hang Yang, Yun Zhang, Yanling Huang, Junping Yu, Hongping Wei*. Degradation of methicillin-resistant Staphylococcus aureus biofilms using a chimeric lysin. Biofouling, vol.17, 2014, pp.1-8.
20. Jinjuan Qiao, Junping Yu, Hang Yang, Hongping Wei*. PCR-based in vitro synthesis of HCV NS3 protease for rapid phenotypic resistance testing of protease inhibitors. Journal of Clinical Microbiology, vol.52, 2014, pp. 1139-1145.
21. Heng Li, Jun Chen, Man Zhou, Xuelei Geng, Junping Yu, Weihua Wang, Xian-En Zhang, Hongping Wei*. Rapid detection of Mycobacterium tuberculosis and pyrazinamide susceptibility related to pncA mutations in sputum specimens through an integrated gene-to-protein function approach. Journal of Clinical Microbiology, vol.52, 2014, pp.260-267.
22. Hang Yang, Yun Zhang, Junping Yu, Yanling Huang, Xian-En Zhang, Hongping Wei*. Novel Chimeric Lysin with High-Level Antimicrobial Activity against Methicillin-Resistant Staphylococcus aureus In Vitro and In Vivo. Antimicrobial Agents and Chemotherapy, vol.58, 2014, pp.536-542.
23. Fengyun Li, Junping Yu, Zhiping Zhang, Zongqiang Cui, Dianbing Wang, Hongping Wei* and Xian-En Zhang*. Use of hGluc/tdTomato pair for sensitive BRET sensing of protease with high solution media tolerance. Talanta, vol.109, 2013, pp.141-146.
24. Dianbing Wang, Bo Tian, Zhiping Zhang, Jiaoyu Deng, Zongqiang Cui, Ruifu Yang, Xuying Wang, Hongping Wei*, Xian-en Zhang*. Rapid detection of Bacillus anthracis spores using a super-paramagnetic lateral-flow immunological detection system. Biosensors & Bioelectronics, 2013,42,661-667.
25. Hang Yang, Dian-Bing Wang, Qiuhua Dong, Zhiping Zhang,Zongqiang Cui, Jiaoyu Deng, Junping Yu, Xian-en Zhang* and Hongping Wei*. Existence of Separate Domains in Lysin PlyG for Recognizing Bacillus anthracis Spores and Vegetative Cells. Antimicrob. Agents Chemother. vol.56(10) 2012,pp. 5031-5039.
26. Junping Yu, Mirong Guan, Fengyun Li, Zhiping Zhang, Chunru Wang, Chunying Shu*, Hongping Wei* and Xian-En Zhang*. Effects of fullerene derivatives on bioluminescence and application for protease detection. Chemical Communication, vol.48, 2012, pp.11011–11013.
27. Fengyun Li, Junping Yu, Zhiping Zhang, Zongqiang Cui, Dianbing Wang, Hongping Wei*, Xian-en Zhang*. Buffer Enhanced Bioluminescence Resonance Energy Transfer Sensor Based on Gaussia Luciferase for in-vitro Detection of Protease. Analytica Chimca Acta, vol.724, 2012, pp.104-110.
28. Man Zhou, Xuelei Geng, Jun Chen, Xude Wang, Dianbing Wang, Jiaoyu Deng, Zhiping Zhang, Weihua Wang, Xian-En Zhang*, Hongping Wei* Rapid Colorimetric Testing for Pyrazinamide Susceptibility of M. tuberculosis by a PCR-Based In-Vitro Synthesized Pyrazinamidase Method, PLoS One, 2011, e27654.

1)      Hongping Wei，Wei Hu，Hang Yang，Junping Yu, Preparation and applications of bioprobes based on S. aureus nanoparticles, 2014.8, Chinese Patent No：201410462482.2. 

2)      Hongping Wei，Hang Yang，Yun Zhang，Junping Yu，Xian-en Zhang，A lysin against S. aureus and its applications，2013.2，Chinese Patent No：201310043837.x.

3)      Hongping Wei，Jinfeng Zhao，Dianbing Wang，Zhiping Zhang，Xian-en Zhang，Preparation and applications of a firefly luciferase , 2012.6，Chinese patent No：ZL200110093484.5

4)      Hongping Wei，Zhou Man, Jinfeng Zhao, Zhiping Zhang，Xian-en Zhang，Rapid detection method for New Delhi Metallo-β-Lactamase，2011.5，Chinese patent No：ZL201110132346.3.

5)      Hongping Wei, Hang Yang, Dianbing Wang, Xian-en Zhang，A peptide for recognizing Bacillus anthracis spores，2012.1，Chinese Patent No：ZL201210006227.8.

6)      Sam F. Y. Li and Hongping Wei, Device and concentration of samples by microcrystallization, Patent No：US6878553，Apr. 12, 2005.

7)      Sam F.Y. Li, Hongping Wei and Guixin Zhang, Potential Gradient Detector, Patent No: US6843901，Jan. 18, 2005.

1) Developing rapid detection methods for bacteria, virus and bacterial resistance.

2) Developing novel lysins and immunotherapy for treating bacterial and viral infections.

2016, The 1st International Lysin meeting, Rockfeller Unviersity, New York, US.

1） Lysin development:

Dr. Danieal Nelson, Maryland University, United States;

Prof. Yuhong Li, Wuhan University, China

2) Detection methods:

Wuhan Scithera Microbiologial Technologies Inc

已指导学生

王云鹏  硕士研究生  071005-微生物学  

张建亮  硕士研究生  071010-生物化学与分子生物学  

汤闻博  硕士研究生  071010-生物化学与分子生物学  

赵金凤  硕士研究生  071010-生物化学与分子生物学  

周满  硕士研究生  071010-生物化学与分子生物学  

耿学磊  硕士研究生  071010-生物化学与分子生物学  

杨航  博士研究生  071005-微生物学  

胡园园  博士研究生  071005-微生物学  

李恒  博士研究生  071005-微生物学  

胡伟  博士研究生  071005-微生物学  

张云  硕士研究生  085238-生物工程  

乔晋娟  博士研究生  071005-微生物学  

滕铁山  博士研究生  071005-微生物学  

王靖  硕士研究生  085238-生物工程  

黄燕玲  硕士研究生  071010-生物化学与分子生物学  

张贇  博士研究生  071005-微生物学  

黄荔  博士研究生  071005-微生物学  

魏翠华  硕士研究生  071005-微生物学  

李云鹏  硕士研究生  071005-微生物学  

现指导学生

罗俊  博士研究生  071005-微生物学  

王淑娟  硕士研究生  071005-微生物学  

张晓旭  博士研究生  071005-微生物学  

李小红  硕士研究生  085238-生物工程  

刘欢  博士研究生  071005-微生物学  

2013 Zhu Li Yue Hua Teacher Award, Chinese Academy of Sciences