1. 创制非天然辅酶偏好型氧化还原酶。

2. 构建非天然辅酶介导的反应体系。

        3. 构建非天然辅酶介导的选择性调控微生物细胞工厂。

   

081703-生物化工

化学生物学
合成生物学

2010-09--2018-06   中国科学院大学   工学博士
2006-09--2010-07   大连大学   工学学士

博士研究生

工学博士

   

2021-07~现在, 中国科学院大连化学物理研究所, 副研究员
2018-11~2021-07,中国科学院大连化学物理研究所, 助理研究员

   

[1] 赵宗保, 王雪颖. 酶促合成烟酰胺胞嘧啶二核苷酸的方法及其应用. CN: CN112695063A, 2021-04-23.

[2] 赵宗保, 刘玉雪, 王雪颖. 一种生物催化还原丙酮酸产D-乳酸的方法. CN: CN111826405A, 2020-10-27.

[3] 赵宗保, 郭潇佳, 王雪颖. 一种利用甲酸还原NAD类似物的方法. CN: CN111808900A, 2020-10-23.

[4] 赵宗保, 刘玉雪, 王雪颖, 刘武军. 一种NAD类似物偏好型氧化还原酶的筛选方法. CN: CN109957602A, 2019-07-02.

[5] 赵宗保, 王雪颖, 刘玉雪. 酶促合成烟酰胺尿嘧啶二核苷酸的方法. CN: CN106884028A, 2017-06-23.

[6] 赵宗保, 王雪颖, 刘玉雪. 酶促合成烟酰胺胞嘧啶二核苷酸的方法. CN: CN106884029A, 2017-06-23.

[7] 赵宗保, 林心萍, 张素芳, 王雪颖. 3-磷酸甘油醛脱氢酶启动子和终止子及其应用. CN: CN105779447A, 2016-07-20.

[8] 赵宗保, 林心萍, 张素芳, 王雪颖. 果糖-1,6-二磷酸醛缩酶启动子和终止子及其应用. CN: CN105779445A, 2016-07-20.

   

[1] Li, Qing, Guo, Xiaojia, Wang, Xueying, Wang, Junting, Wan, Li, Xue, Haizhao, Zhao, Zongbao K. Creation of cytochrome P450 catalysis depending on a non-natural cofactor for fatty acid hydroxylation. JOURNAL OF ENERGY CHEMISTRY[J]. 2023, 79: 31-36, http://dx.doi.org/10.1016/j.jechem.2022.12.021.
[2] Liu, Yuxue, Li, Zhuoya, Guo, Xiaojia, Wang, Xueying, Zhao, Zongbao K. A phosphite-based screening platform for identification of enzymes favoring nonnatural cofactors. SCIENTIFIC REPORTS[J]. 2022, 12(1): [3] Wan, Li, Wang, Xueying, Hu, Yinghan, Li, Qing, Zhao, Zongbao K. Gram-scale biocatalytic preparation of the non-natural cofactor nicotinamide cytosine dinucleotide. TETRAHEDRON LETTERS[J]. 2022, 88: http://dx.doi.org/10.1016/j.tetlet.2021.153568.
[4] 梁世玉, 万里, 郭潇佳, 王雪颖, 吕力婷, 胡英菡, 赵宗保. 构建可合成非天然辅酶的圆红冬孢酵母工程菌. 中国生物工程杂志. 2022, 42(5): 58-68, https://d.wanfangdata.com.cn/periodical/swgcjz202205013.
[5] Xueying Wang, Yanbin Feng, Xiaojia Guo, Qian Wang, Siyang Ning, Qing Li, Junting Wang, Lei Wang, Zongbao K Zhao. Creating enzymes and self-sufficient cells for biosynthesis of the non-natural cofactor nicotinamide cytosine dinucleotide. NATURE COMMUNICATIONS[J]. 2021, 12(1): http://dx.doi.org/10.1038/s41467-021-22357-z.
[6] Guo, Xiaojia, Liu, Yuxue, Wang, Qian, Wang, Xueying, Li, Qing, Liu, Wujun, Zhao, Zongbao K. Non-natural Cofactor and Formate-Driven Reductive Carboxylation of Pyruvate. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION[J]. 2020, 59(8): 3143-3146, https://www.webofscience.com/wos/woscc/full-record/WOS:000508220700001.
[7] Guo, Xiaojia, Wang, Xueying, Liu, Yuxue, Li, Qing, Wang, Junting, Liu, Wujun, Zhao, Zongbao K. Structure-Guided Design of Formate Dehydrogenase for Regeneration of a Non-Natural Redox Cofactor. CHEMISTRY-A EUROPEAN JOURNAL[J]. 2020, 26(70): 16611-16615, https://www.webofscience.com/wos/woscc/full-record/WOS:000590296100001.
[8] Guo, Xiaojia, Feng, Yanbin, Wang, Xueying, Liu, Yuxue, Liu, Wujun, Li, Qing, Wang, Junting, Xue, Song, Zhao, Zongbao K. Characterization of the substrate scope of an alcohol dehydrogenase commonly used as methanol dehydrogenase. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS[J]. 2019, 29(12): 1446-1449, http://cas-ir.dicp.ac.cn/handle/321008/165514.
[9] Guo, Xiaojia, Shen, Hongwei, Liu, Yuxue, Wang, Qian, Wang, Xueying, Peng, Chang, Liu, Wujun, Zhao, Zongbao K. Enabling Heterologous Synthesis of Lupulones in the Yeast Saccharomyces cerevisiae. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY[J]. 2019, 188(3): 787-797, https://www.webofscience.com/wos/woscc/full-record/WOS:000471851400013.
[10] Liu, Yuxue, Feng, Yanbin, Wang, Lei, Guo, Xiaojia, Liu, Wujun, Li, Qing, Wang, Xueying, Xue, Song, Zhao, Zongbao Kent. Structural Insights into Phosphite Dehydrogenase Variants Favoring a Non-natural Redox Cofactor. ACS CATALYSIS[J]. 2019, 9(3): 1883-1887, http://cas-ir.dicp.ac.cn/handle/321008/165860.
[11] Sun, Wenyi, Yang, Xiaobing, Wang, Xueying, Jiao, Xiang, Zhang, Sufang, Luan, Yushi, Zhao, Zongbao K. Developing a flippase-mediated maker recycling protocol for the oleaginous yeast Rhodosporidium toruloides. BIOTECHNOLOGY LETTERS[J]. 2018, 40(6): 933-940, http://cas-ir.dicp.ac.cn/handle/321008/167164.
[12] Wang, Xueying, Wang, Lei, Lin, Xinping, Yang, Xiaobing, Liu, Wujun, Zhao, Zongbao K. Visualizing Soluble Protein Mutants by Using Monomeric Red Fluorescent Protein as a Reporter for Directed Evolution. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY[J]. 2018, 185(1): 81-90, http://cas-ir.dicp.ac.cn/handle/321008/167210.
[13] Wang, Lei, Ji, Debin, Liu, Yuxue, Wang, Qian, Wang, Xueying, Zhou, Yongjin J, Zhang, Yixin, Liu, Wujun, Zhao, Zongbao K. Synthetic Cofactor-Linked Metabolic Circuits for Selective Energy Transfer. ACS CATALYSIS[J]. 2017, 7(3): 1977-1983, http://cas-ir.dicp.ac.cn/handle/321008/169352.
[14] Sun, Wenyi, Yang, Xiaobing, Wang, Xueying, Lin, Xinping, Wang, Yanan, Zhang, Sufang, Luan, Yushi, Zhao, Zongbao K. Homologous gene targeting of a carotenoids biosynthetic gene in Rhodosporidium toruloides by Agrobacterium-mediated transformation. BIOTECHNOLOGYLETTERS[J]. 2017, 39(7): 1001-1007, https://www.webofscience.com/wos/woscc/full-record/WOS:000402394300008.
[15] Wang, Xueying, Zhou, Yongjin J, Wang, Lei, Liu, Wujun, Liu, Yuxue, Peng, Chang, Zhao, Zongbao K. Engineering Escherichia coli Nicotinic Acid Mononucleotide Adenylyltransferase for Fully Active Amidated NAD Biosynthesis. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2017, 83(13): https://www.webofscience.com/wos/woscc/full-record/WOS:000403495700023.
[16] 王磊, 刘武军, 刘玉雪, 王雪颖, 赵宗保. 正交氧化还原体系及其应用. 中国科学 生命科学[J]. 2015, 45: 969-, http://cas-ir.dicp.ac.cn/handle/321008/148241.
[17] Zhang, Yixin, Liu, Wujun, Sohai, Muhammad, Wang, Xueying, Liu, Yuxue, Zhao, Zongbao K. Enzymatic Allylation of Catechols. CHEMISTRY LETTERS[J]. 2015, 44(7): 949-951, http://cas-ir.dicp.ac.cn/handle/321008/146389.
[18] Lin, Xinping, Wang, Yanan, Zhang, Sufang, Zhu, Zhiwei, Zhou, Yongjin J, Yang, Fan, Sun, Wenyi, Wang, Xueying, Zhao, Zongbao K. Functional integration of multiple genes into the genome of the oleaginous yeast Rhodosporidium toruloides. FEMS YEAST RESEARCH[J]. 2014, 14(4): 547-555, http://cas-ir.dicp.ac.cn/handle/321008/144055.

   

（ 1 ） 人工辅酶依赖型1-脱氧木酮糖-5-磷酸还原异构酶创制研究, 负责人, 国家任务, 2020-01--2022-12

（1）构建非天然辅酶NCD自给工程菌   中国化学会第32届学术年会   2021-04-19