Systems and synthetic biology of industrial microorganisms; Design and develepment of industrial strains

系统生物技术

2001.10--2004.10 Technical University of Braunschweig / German Research Center for Biotechnology (GBF), doctor

1993-09--1996-07 山东大学 硕士研究生

1993.09--1996.07 Shandong University, master

1989-09--1993-07 山东大学 学士

1989.09--1993.07 Shandong University, bachelor

2008.03-- present, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, PI

2004-10--2008-03 Helmholtz Center for Infection Research, Germany 博士后

2004.10--2008.03   Helmholtz Center for Infection Research,  postdoc

1996-07--2000-10 山东大学微生物技术国家重点实验室 讲师

1996.07--2000.10   State Key Laboratory of Microbial Technology, Shandong University, Lecturer

(1) Yu Wang*, Haijiao Cheng, Yang Liu, Ye Liu, Xiao Wen, Kun Zhang,Xiaomeng Ni, Ning Gao, Liwen Fan, Zhihui Zhang, Jiao Liu, Jiuzhou Chen, Lixian Wang, Yanmei Guo, Ping Zheng*, Meng Wang*, Jibin Sun and Yanhe Ma. In-situ generation of large numbers of genetic combinations for metabolic reprogramming via crispr-guided base editing. Nature Communications, 2021,12(1):678. 

(2) Yu Wang*, Ye Liu, Ping Zheng, Jibin Sun, and Meng Wang*. Microbial Base Editing: A Powerful Emerging Technology for Microbial Genome Engineering. Trends in Biotechnology, 2021, 39(2):165-180.

(3) Zhihui Zhang,Yu Wang*,Ping Zheng*, and Jibin Sun. Promoting Lignin Valorization by Coping with Toxic C1 Byproducts. Trends in Biotechnology, 2020, 39(4):331-335.

(4) Xue Sun#, Qinggang Li#, Yu Wang#, Wenjuan Zhou, Yanmei Guo,Jiuzhou Chen, Ping Zheng*, Jibin Sun*, Yanhe Ma. Isoleucyl-tRNA synthetase mutant based whole-cell biosensor for high-throughput selection of isoleucine overproducers. Biosensors and Bioelectronics, 2020, 172.

(5) Yu Wang, Liwen Fan, Philibert Tuyishime, Ping Zheng*, Jibin Sun*. Synthetic Methylotrophy: A Practical Solution for Methanol-Based Biomanufacturing. Trends in Biotechnology, 2020, 38(6):650-666.

(6) Jiuzhou Chen#, Yu Wang# , Xuan Guo, Deming Rao, Wenjuan Zhou, Ping Zheng* , Jibin Sun* and Yanhe. Efficient bioproduction of 5‑aminolevulinic acid, a promising biostimulant and nutrient, from renewable bioresources by engineered Corynebacterium glutamicum. Biotechnol Biofuels, 2020, 13(1):41.

(7) Lihui Zhang#, Xiaomei Zheng#, Timothy C. Cairns, Zhidan Zhang, Depei Wang*, Ping Zheng*and Jibin Sun. Disruption or reduced expression of the orotidine‑5′‑decarboxylase gene pyrG increases citric acid production: a new discovery during recyclable genome editing in Aspergillus niger. Microbial Cell Factories, 2020, 19(1):76.

(8) Mingyue Li#, Jiuzhou Chen#, Yu Wang, Jiao Liu, Jingwen Huang, Ning Chen,Ping Zheng* and Jibin Sun*. Efficient Multiplex Gene Repression by CRISPR-dCpf1 in Corynebacterium glutamicum. Frontiers in Bioengineering and Biotechnology, 2020, 8:357.

(9) Yu Wang, Liwen Fan, Philibert Tuyishime, Jiao Liu, Kun Zhang, Ning Gao, Zhihui Zhang,Xiaomeng Ni, Jinhui Feng, Qianqian Yuan, Hongwu Ma, Ping Zheng*, Jibin Sun* and Yanhe Ma. Adaptive laboratory evolution enhances methanol tolerance and conversion in engineered Corynebacterium glutamicum. Communications Biology, 2020, 3(1):217.

(10) Dehu Sun#,Jiuzhou Chen#,Yu Wang,Mingyue Li,Deming Rao,Yanmei Guo, Ning Chen, Ping Zheng*,Jibin. Metabolic Engineering of Corynebacterium glutamicum by Synthetic Small Regulatory RNAs. Journal of Industrial Microbiology & Biotechnology, 2019, 46(2):203-208.

(11) Jing Zhao#, Chao Liu#, Xuan Guo, Jinyu Wang, Haiping Liu, Ping Zheng*,Jibin Sun*, Yanhe Ma. Efficient production of trans-3-hydroxyproline by a bacterial trans-3-proline hydroxylase and characterization of enzymatic properties. Biochemical Engineering Journal, 2019, 147:57-61.

(12) Timothy C. Cairns, Xiaomei Zheng , Ping Zheng, Jibin Sun, Vera Meyer*. Moulding the mould: understanding and reprogramming filamentous fungal growth and morphogenesis for next generation cell factories. Biotechnology for Biofuels, 2019, 12:77.

(13) Zhenyu Tong#, Xiaomei Zheng#, Yi Tong, Yong‑Cheng Shi and Jibin Sun*. Systems metabolic engineering for citric acid production by Aspergillus niger in the post‑genomic era. Microbial Cell Factories, 2019, 18(1):28.

(14) Chengchao Zhu#, Jiuzhou Chen#, Yu Wang, Lixian Wang,Xuan Guo,Ning Chen, Ping Zheng*, Jibin Sun*, Yanhe Ma. Enhancing 5‐aminolevulinic acid tolerance and production by engineering the antioxidant defense system of Escherichia coli. Biotechnology and Bioengineering, 2019, 116(8):2018-2028.

(15) Timothy C. Cairns#, Claudia Feurstein#, Xiaomei Zheng, Ping Zheng, Jibin Sun* and Vera Meyer*. A quantitative image analysis pipeline for the characterization of filamentous fungal morphologies as a tool to uncover targets for morphology engineering: a case study using aplD in Aspergillus niger. Biotechnology for Biofuels, 2019,12:149.

(16) Xiaowei Wang#, Qinggang Li#, Cunmin Sun, Zhen Cai, Xiaomei Zheng, Xuan Guo, Xiaomeng Ni,Wenjuan Zhou, Yanmei Guo, Ping Zheng*, Ning Chen, Jibin Sun* , Yin Li and Yanhe Ma. GREACE-assisted adaptive laboratory evolution in endpoint fermentation broth enhances lysine production by Escherichia coli. Microbial Cell Factories, 2019, 18(1):106.

(17) Yu Wang#, Ye Liu#, Junwei Li, Yi Yang, Xiaomeng Ni, Haijiao Cheng, Teng Huang, Yanmei Guo, Hongwu Ma, Ping Zheng*, Meng Wang*, Jibin Sun, Yanhe Ma. Expanding targeting scope, editing window, and base transition capability of base editing in Corynebacterium glutamicum. Biotechnology and Bioengineering, 2019, 116(11):3016-3029.

(18) Taiwo Dele-Osibanjo#, Qinggang Li#, Xiaoli Zhang#, Xuan Guo, Jinhui Feng, Jiao Liu, Xue Sun, Xiaowei Wang, Wenjuan Zhou, Ping Zheng*, Jibin Sun*,Yanhe Ma. Growth-coupled evolution of phosphoketolase to improve l-glutamate production by Corynebacterium glutamicum. Applied Microbiology and Biotechnology, 2019, 103(20):8413-8425.

(19) Timothy C. Cairns, Claudia Feurstein, Xiaomei Zheng, Li Hui Zhang, Ping Zheng,Jibin Sun* and Vera Meyer*. Functional exploration of co‑expression networks identifies a nexus for modulating protein and citric acid titres in Aspergillus niger submerged culture. Fungal Biology and Biotechnology, 2019, 6:18.

(20) Xiaomei Zheng, Ping Zheng*, Kun Zhang, Timothy C. Cairns, Vera Meyer, Jibin Sun*, and Yanhe Ma. 5S rRNA Promoter for Guide RNA Expression Enabled Highly Efficient CRISPR/Cas9 Genome Editing in Aspergillus niger. ACS Synth Biol, 8(7):1568-1574.

(21) Yu Wang#, Ye Liu#, Jiao Liu, Yanmei Guo, Liwen Fan, Xiaomeng Ni, Xiaomei Zheng, Meng Wang*, Ping Zheng*, Jibin Sun*, Yanhe Ma. MACBETH: multiplex automated Corynebacterium glutamicum base editing method. Metabolic Engineering, 2018, 47:200-210.

(22) Qiongqiong Zhang#, Xiaomei Zheng#, Yu Wang, Jiandong Yu, Zhidan Zhang, Taiwo Dele-Osibanjo, Ping Zheng*, Jibin Sun*, Shiru Jia, Yanhe Ma. Comprehensive Optimization of the Metabolomics Methodology for Metabolite Profiling of Corynebacterium glutamicum. Applied Microbiology and Biotechnology, 2018,102(16):7113-7121.

(23) Yu Wang, Guoqiang Cao, Deyu Xu, Liwen Fan, Xinyang Wu, Xiaomeng Ni, Shuxin Zhao, Ping Zheng*, Jibin Sun*, Yanhe Ma. A Novel L-Glutamate Exporter of Corynebacterium glutamicum. Appl Environ Microbiol, 2018, 84(6):e02691-17.

(24) Xiaomei Zheng, Ping Zheng*, Jibin Sun*, Zhang Kun, Yanhe Ma. Heterologous and endogenous U6 snRNA promoters enabled CRISPR/Cas9 mediated genome editing in Aspergillus niger. Fungal Biology and Biotechnology, 2018, 5:2.

(25) Philibert Tuyishime#,Yu Wang#, Liwen Fan, Qiongqiong Zhang, Qinggang Li, Ping Zheng*, Jibin Sun*,Yanhe Ma. Engineering Corynebacterium glutamicum for methanol-dependent growth and glutamate production. Metabolic Engineering, 2018, 49:220-231.

(26) Jiao Liu#, Yu Wang#, Yujiao Lu, xiaomeng Ni, Xuan Guo, Jing Zhao, Jiuzhou Chen, Taiwo

Dele-Osibanjo, Ping Zheng*, Jibin Sun*, and Yanhe Ma. Mutations on Peptidoglycan Synthesis Gene ponA Improve Electro-transformation Efficiency of Corynebacterium glutamicum ATCC 13869. Applied and Environmental Microbiology, 2018, 84(24):e02225-18.

(27) Chao Liu#, Jing Zhao#, Jiao Liu, Xuan Guo, Deming Rao, Haiping Liu,Ping Zheng* ,Jibin Sun* ,Yanhe Ma. Simultaneously improving the activity and thermostability of a new proline 4-hydroxylase by loop grafting and site-directed mutagenesis. Applied Microbiology and Biotechnology, 2019, 103(1):265-277.

(28) Xiaomei Zheng, Jiandong Yu, Timothy C. Cairns, Lihui Zhang, Zhidan Zhang, Qiongqiong Zhang, Ping Zheng,* Jibin Sun,* and Yanhe Ma. Comprehensive Improvement of Sample Preparation Methodologies Facilitates Dynamic Metabolomics of Aspergillus niger. Biotechnology Journal, 2019, 14(3):e1800315.

(29) Liwen Fan#,Yu Wang#,Philibert Tuyishime,Ning Gao Qinggang Li, Ping Zheng*, Jibin Sun*,  Yanhe Ma. Engineering Artificial Fusion Proteins for Enhanced Methanol Bioconversion. ChemBioChem, 2018, 19(23):2465-2471.

(30) Xing-Chu Wang#, Jiao Liu#, Jing Zhao, Xiao-Meng Ni, Ping Zheng, Xuan Guo, Cun-Min Sun, Ji-Bin Sun* and Yan-He Ma. Efficient production of trans-4-hydroxy-L-proline from glucose using a new transproline 4-hydroxylase in Escherichia coli. J Biosci Bioeng, 2018, 126(4):470-477.

(31) Xiaolu Wang#, Yu Wang#, Jiao Liu#, Qinggang Li, Zhidan Zhang, Ping Zheng, Fuping Lu, and Jibin Sun*. Biological conversion of methanol by evolved escherichia coli carrying a linear methanol assimilation pathway. Bioresources & Bioprocessing, 2017, 4(1):41.

(32) Jiao Liu#, Yu Wang#, Yujiao Lu, Ping Zheng*, Jibin Sun* and Yanhe Ma. Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium glutamicum. Microb Cell Fact, 2017,16(1):205.

(33) Deyu Xu#, Jing Zhao#, Guoqiang Cao, Jinyu Wang, Qinggang Li, Ping Zheng*, Shuxin Zhao, Jibin Sun. Removal of feedback inhibition of Corynebacterium glutamicum phosphoenolpyruvate carboxylase by addition of a short terminal peptide. Biotechnology and Bioprocess Engineering, 2018, 23(1):72-78.

(34)王晓璐，王钰，刘娇，路福平*，郑平*。利用定向进化提高基因工程大肠杆菌的甲醇利用能力。生物技术通报，2017，33（9）：101-109。

(35) Yan Wang#, Qinggang Li#, Ping Zheng*, Yanmei Guo, Lixian Wang, Tongcun Zhang, Jibin Sun*, Yanhe Ma. Evolving the L-lysine high-producing strain of Escherichia coli using a newly developed high-throughput screening method. Journal of Industrial Microbiology & Biotechnology, 2016, 43(9):1227-35.

(36) Fengyu Kou#, Jing Zhao#, Jiao Liu, Jie Shen, Qin Ye, Ping Zheng, Zhimin Li*, Jibin Sun*, Yanhe Ma. Characterization of a new lysine decarboxylase from Aliivibrio salmonicida for cadaverine production at alkaline pH. Journal of Molecular Catalysis B: Enzymatic, 2016, 133:S88-S94.

(37) Yong Wu, Pengpeng Li, Ping Zheng, Wenjuan Zhou, Ning Chen, Jibin Sun*. Complete genome sequence of Corynebacterium glutamicum B253, a Chinese lysine-producing strain. Journal of Biotechnology, 2015, 207:10-1.

(38)张晓立#，郑小梅#，满云，罗虎，于建东，郑平，刘浩，孙际宾*。黑曲霉柠檬酸工业菌株原生质体制备与转化。生物技术通报，2015，31（3）：171-177。

(39) 郑小梅，张晓立，于建东，郑平，孙际宾*。CRISPR-Cas9介导的基因组编辑技术的研究进展。生物技术进展，2015，5（1）：1-9。

(40) Ya’nan Liu#, Qinggang Li#, Ping Zheng*, Zhidan Zhang, Yongfei Liu, Cunmin Sun, Guoqiang Cao, Wenjuan Zhou, Xiaowei Wang, Dawei Zhang, Tongcun Zhang, Jibin Sun* and Yanhe Ma. Developing a high-throughput screening method for threonine overproduction based on an artificial promoter. Microb Cell Fact, 2015, 14:121.

(41) Feng Q He*, Wei Wang, Ping Zheng, Padhmanand Sudhakar, Jibin Sun, An-Ping Zeng*. Essential O2-responsive genes of Pseudomonas aeruginosa and their network revealed by integrating dynamic data from inverted conditions. Integrative Biology, 2014, 6(2):215-23.

(42)徐自祥，郑平，孙际宾*。全细胞网络重建与细胞工厂设计。生物化学与生物物理进展, 2014，41（2）：105-114。

(43)蒲伟，陈久洲，孙村民，陈宁，孙际宾，郑平*，马延和。琥珀酸脱氢酶或琥珀酰辅酶A 合成酶缺失促进大肠杆菌积累5-氨基乙酰丙酸。生物工程学报，2013，29（10）：1494-1503。

(44) Zixiang Xu, Ping Zheng, Jibin Sun*, Yanhe Ma. ReacKnock: Identifying reaction deletion strategies for microbial strain optimization based on genome-scale metabolic network. PLOS ONE, 2013, 8(12):e72150.

(45) Yan Zhu, Jiangning Song, Zixiang Xu, Jibin Sun, Yanping Zhang, Yin Li*, Yanhe Ma. Development of Thermodynamic Optimum Searching (TOS) to Improve the Prediction Accuracy of Flux Balance Analysis. Biotechnol Bioeng, 2013, 110(3):914-23.

(46)吴新阳#，裴广胜#，郑小梅，曹国强，刘娇，郑平，王敏，孙际宾*。不同抑制剂对谷氨酸棒状杆菌磷酸烯醇式丙酮酸羧化酶酶活的影响。生物技术通报，2013，0 （7）：172-178。

(47)姜安娜，曹名锋，李庆刚，郑平，杨洪江，孙际宾*。一种改进的赖氨酸浓度测定方法。生物技术通报，2013，0（2：190-194。

(48) Lin Chen, Zhen Chen, Ping Zheng, Jibin Sun*, An-Ping Zeng*. Study and reengineering of the binding sites and allosteric regulation of biosynthetic threonine deaminase by isoleucine and valine in Escherichia coli. Appl Microbiol Biotechnol, 2013, 97(7):2939-49. 

(49) Zixiang Xu, Xiao Sun*, Jibin Sun*.Construction and Analysis of the Model of Energy Metabolism in E. coli. PLOS ONE, 2013, 8(1):e55137.

(50) Lilu Zhang#, Jiuzhou Chen#, Ning Chen, Jibin Sun, Ping Zheng*, Yanhe Ma. Cloning of two 5-Aminolevulinic Acid Synthase Isozymes HemA and HemO from Rhodopseudomonas palustris with favorable characters for 5-aminolevulinic acid production. Biotechnology Letters, 2013, 35(5):763-8.

(51) Feng Geng, Zhen Chen, Ping Zheng, Jibin Sun*, An-Ping Zeng*. Exploring the allosteric mechanism of dihydrodipicolinate synthase by reverse engineering of the allosteric inhibitor binding sites and its application for lysine production. Applied Microbiology and Biotechnology, 2012,97(5):1963-71.

(52)姜金国，宋理富，郑平，贾士儒，孙际宾*。谷氨酸棒状杆菌集成细胞网络的构建与结构分析。生物工程学报，2012，28（5）：577-591。

(53) Lifu Song, Padhmanand Sudhakar, Wei Wang*, Georg Conrads, Anke Brock, Jibin Sun, Irene Wagner-Döbler, An-Ping Zeng*.A genome-wide study of two-component signal transduction systems in eight newly sequenced mutans streptococci strains, BMC Genomics, 2012, 通讯作者

(54) Rosarin Rujananon, Poonsuk Prasertsan, Amornrat Phongdara, Tanate Panrat, Jibin Sun,  Sugima Rapper, An-Ping Zeng. Construction of recombinant e.coli expressing fusion protein to produce 1,3-propanediol. World Academy of Science Engineering & Technology, 2013, 76(76), 507.

(55) Zhen Chen, Sugima Rappert, Jibin Sun, An-Ping Zeng*. Integrating molecular dynamics and co-evolutionary analysis for reliable target prediction and deregulation of the allosteric inhibition of aspartokinase for amino acid production. Journal of Biotechnology, 2011, 154(4), 248-254.

(56) Fei Su, Bo Yu, Jibin Sun, Hong-Yu Qu, Bo Zhao, Linmi Wang, Jiayang Qin, Hongzhi Tang, Fei Tao, Jarek Michael, Scharfe Maren, Cuiqing Ma,Yanhe Ma, Ping Xu*. Genome sequence of the thermophilic strain bacillus coagulans 2-6, an efficient producer of high-optical-purity l-lactic acid. Journal of Bacteriology, 2011, 193(17).

(57) Bo Yu, Jibin Sun, Rajesh Reddy Bommareddy, Lifu Song, An-Ping Zeng*. A novel (2r,3r)-2,3-butanediol dehydrogenase from an industrially potential strain paenibacillus polymyxa atcc12321. Appl Environ Microbiol, 2011, 77(12).

(58) Zhen Chen, Weiqian Meyer, Sugima Rappert, Jibin Sun, An-Ping Zeng*. Coevolutionary analysis enabled rational deregulation of allosteric enzyme inhibition in corynebacterium glutamicum for lysine production. Applied & Environmental Microbiology, 2011, 77(13), 4352.

(59) Michael Stelzer, Jibin Sun, Tom Kamphans, Sándor P Fekete*, An-Ping Zeng*. An extended bioreaction database that significantly improves reconstruction and analysis of genome-scale metabolic networks. Integrative Biology, 2011, 3(11), 1071-1086.

(60) Y-H Percival Zhang*, Jibin Sun, Jian-Jiang Zhong. Biofuel production by in vitro synthetic enzymatic pathway biotransformation. Current Opinion in Biotechnology, 2010, 21(5), 663-669.

(61) Maria G*, Zhongxia Xu, Jibin Sun . (2012). Multi-objective MINLP Optimization Used to Identify Theoretical Gene Knockout Strategies for E. coli Cell. Chem.biochem.eng.q, 2012, 25(4).

(62) Xin Lu, Jibin Sun, Manfred Nimtz, Josef Wissing, An-Ping Zeng, Ursula Rinas*. The intra- and extracellular proteome of aspergillus niger growing on defined medium with xylose or maltose as carbon substrate. Microbial Cell Factories, 2010, 9(1), 1-13.

(63) Marcin Bizukojc*, David Dietz, Jibin Sun, An-Ping Zeng*. Metabolic modelling of syntrophic-like growth of a 1,3-propanediol producer, clostridium butyricum, and a methanogenic archeon, methanosarcina mazei, under anaerobic conditions. Bioprocess & Biosystems Engineering, 33(4), 507.

(64)郭艳梅，郑平，孙际宾*。黑曲霉作为细胞工厂：知识准备与技术基础。生物工程学报, 2010，26（10）：1410-1418。

(65) Michel Flipphi, Jibin Sun, Xavier Robellet, Levente Karaffa, Erzsébet Fekete, An-Ping Zeng, Christian P Kubicek*. Biodiversity and evolution of primary carbon metabolism in aspergillus nidulans and other aspergillus spp. Fungal Genetics & Biology, 2009,46(1-supp-S), S19-S44.

已指导学生

戴寅飞  硕士研究生  071005-微生物学  

贾斌  硕士研究生  071005-微生物学  

王平平  硕士研究生  085238-生物工程  

毕振华  硕士研究生  085238-生物工程  

邱华敏  硕士研究生  085238-生物工程  

王兴初  博士研究生  071005-微生物学  

崔继龙  硕士研究生  071005-微生物学  

王丽冉  硕士研究生  071005-微生物学  

谭焕波  博士研究生  071010-生物化学与分子生物学  

刘超  博士研究生  071005-微生物学  

王金玉  硕士研究生  085238-生物工程  

孙雪  博士研究生  071005-微生物学  

陆育娇  硕士研究生  071005-微生物学  

张昆  硕士研究生  085238-生物工程  

张佟佟  博士研究生  071005-微生物学  

现指导学生

高宁  博士研究生  071005-微生物学  

陈怡雪  硕士研究生  071005-微生物学  

祁瑞轩  硕士研究生  086000-生物与医药