1.蛋白质结构和动态性的研究

2.酶催化机理解析和理性设计

   

071010-生物化学与分子生物学

蛋白质结构和动力学的研究
酶催化机理解析和理性设计

2017-09--2020-12   中国科学院大学   博士
2007-09--2010-06   南京师范大学   硕士
2003-09--2007-06   湖南文理学院   本科

研究生

博士

   

2018-12~现在, 中国科学院青岛生物能源与过程研究所, 副研究员
2013-12~2018-12,中国科学院青岛生物能源与过程研究所, 助理研究员
2011-03~2013-12,中国科学院青岛生物能源与过程研究所, 研究研习员

   

[1] 姚礼山, 梅硕·哈杜什·贝赫, 宋乡飞, 王梦婷. 具有高酶活性与高热稳定性的葡聚糖单加氧酶及其制备方法与应用. 202310083991.3, 2023-02-09.
[2] 姚礼山, 宋乡飞, 王业飞, 张树军, 张雨, 洪景波. 一种改良性能的纤维素内切酶Cel7B突变体. CN: CN104560922A, 2015-04-29.
[3] 姚礼山, 宋乡飞, 王业飞. 一种改良性能的黄素单核苷酸结合荧光蛋白质. CN: CN103421096A, 2013-12-04.

   

[1] 梅硕·哈杜什·贝赫, 宋乡飞, 姚礼山. Improving the Enzymatic Activity and Stability of a Lytic Polysaccharide Monooxygenase. Int. J. Mol. Sci[J]. 2023, 24: 8963-8978, [2] Song xiangfei, Wang mengting, Chen xiaoxu, Zhang xueying, Yang ying, Liu zhijun, Yao lishan. Quantifying Protein Electrostatic Interactions in Cells by Nuclear Magnetic Resonance Spectroscopy. J. Am. Chem. Soc[J]. 2021, 143: 19606-19613, [3] Song, Xiangfei, An, Liaoyuan, Wang, Mengting, Chen, Jingfei, Liu, Zhijun, Yao, Lishan. Osmolytes Can Destabilize Proteins in Cells by Modulating Electrostatics and Quinary Interactions. ACS CHEMICAL BIOLOGY[J]. 2021, 16(5): 864-871, http://dx.doi.org/10.1021/acschembio.1c00024.
[4] Song, Xiangfei, Lv, Tianhang, Chen, Jingfei, Wang, Jia, Yao, Lishan. Characterization of Residue Specific Protein Folding and Unfolding Dynamics in Cells. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY[J]. 2019, 141(29): 11363-11366, [5] Zhang, Ning, Wang, Yefei, An, Liaoyuan, Song, Xiangfei, Huang, Qingshan, Liu, Zhijun, Yao, Lishan. Entropy Drives the Formation of Salt Bridges in the Protein GB3. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION[J]. 2017, 56(26): 7601-7604, http://www.irgrid.ac.cn/handle/1471x/1755774.
[6] Xiangfei Song, Shujun Zhang, Yefei Wang, Jingwen Li, Chunyan He, Lishan Yao. Corrigendum to ‘A kinetic study of Trichoderma reesei Cel7B catalyzed cellulose hydrolysis’ Enzyme Microb. Technol. 87–88 (2016) 9–16. ENZYME AND MICROBIAL TECHNOLOGY. 2016, 92: 117-117, http://dx.doi.org/10.1016/j.enzmictec.2016.08.008.
[7] Wang, Yefei, Zhang, Shujun, Song, Xiangfei, Yao, Lishan. Cellulose Chain Binding Free Energy Drives the Processive Move of Cellulases on the Cellulose Surface. BIOTECHNOLOGY AND BIOENGINEERING[J]. 2016, 113(9): 1873-1880, http://ir.qibebt.ac.cn/handle/337004/8479.
[8] Song, Xiangfei, Zhang, Shujun, Wang, Yefei, Li, Jingwen, He, Chunyan, Yao, Lishan. A kinetic study of Trichoderma reesei Cel7B catalyzed cellulose hydrolysis. ENZYME AND MICROBIAL TECHNOLOGY[J]. 2016, 87-88(1): 9-16, http://dx.doi.org/10.1016/j.enzmictec.2016.02.008.
[9] Wang, Yefei, Song, Xiangfei, Zhang, Shujun, Li, Jingwen, Shu, Zhiyu, He, Chunyan, Huang, Qingshan, Yao, Lishan. Improving the activity of Trichoderma reesei cel7B through stabilizing the transition state. BIOTECHNOLOGY AND BIOENGINEERING[J]. 2016, 113(6): 1171-1177, http://dx.doi.org/10.1002/bit.25887.
[10] Song, Xiangfei, Zhang, Shujun, Wang, Yefei, Li, Jingwen, He, Chunyan, Yao, Lishan. A kinetic study of Trichoderma reesei Cel7B catalyzed cellulose hydrolysis (vol 87, pg 9, 2016). ENZYME AND MICROBIAL TECHNOLOGY. 2016, 92: 117-117, http://ir.qibebt.ac.cn/handle/337004/8486.
[11] Chen, Chao, Cui, Zhenling, Song, Xiangfei, Liu, YaJun, Cui, Qiu, Feng, Yingang. Integration of bacterial expansin-like proteins into cellulosome promotes the cellulose degradation. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2016, 100(5): 2203-2212, http://www.irgrid.ac.cn/handle/1471x/1059896.
[12] Song, Xiangfei, Wang, Yefei, Zhang, Shujun, Yan, Shihai, Li, Tong, Yao, Lishan. Characterization of the Dielectric Constant in the Trichoderma reesei Cel7B Active Site. JOURNAL OF CHEMICAL INFORMATION AND MODELING[J]. 2015, 55(7): 1369-1376, https://www.webofscience.com/wos/woscc/full-record/WOS:000358821300010.
[13] Zhang, Shujun, Wang, Yefei, Song, Xiangfei, Hong, Jingbo, Zhang, Yu, Yao, Lishan. Improving Trichoderma reesei Cel7B Thermostability by Targeting the Weak Spots. JOURNAL OF CHEMICAL INFORMATION AND MODELING[J]. 2014, 54(10): 2826-2833, http://dx.doi.org/10.1021/ci500339v.
[14] Song, Xiangfei, Wang, Yefei, Shu, Zhiyu, Hong, Jingbo, Li, Tong, Yao, Lishan. Engineering a More Thermostable Blue Light Photo Receptor Bacillus subtilis YtvA LOV Domain by a Computer Aided Rational Design Method. PLOS COMPUTATIONAL BIOLOGY[J]. 2013, 9(7): http://www.irgrid.ac.cn/handle/1471x/830045.
[15] Wang, Shan, Cui, GuZhen, Song, XiangFei, Feng, Yingang, Cui, Qiu. Efficiency and Stability Enhancement of Cis-epoxysuccinic Acid Hydrolase by Fusion with a Carbohydrate Binding Module and Immobilization onto Cellulose. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY[J]. 2012, 168(3): 708-717, http://www.irgrid.ac.cn/handle/1471x/519013.
[16] Song, Xiangfei, Xue, Yemin, Wang, Qilei, Wu, Xixi. Comparison of Three Thermostable beta-Glucosidases for Application in the Hydrolysis of Soybean Isoflavone Glycosides. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY[J]. 2011, 59(5): 1954-1961, http://dx.doi.org/10.1021/jf1046915.
[17] Xue, Yemin, Song, Xiangfei, Yu, Jinjin. Overexpression of beta-glucosidase from Thermotoga maritima for the production of highly purified aglycone isoflavones from soy flour. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY[J]. 2009, 25(12): 2165-2172, http://dx.doi.org/10.1007/s11274-009-0121-4.
[18] Xue, Yemin, Yu, Jinjin, Song, Xiangfei, Peng, Jingjing, Shao, Weilan. Over-expression of xylanolytic alpha-glucuronidase from Thermotoga maritima in the pHsh system of Escherichia coli for the production of xylobiose from xylan. AFRICAN JOURNAL OF BIOTECHNOLOGY[J]. 2008, 7(14): 2454-2461, https://www.webofscience.com/wos/woscc/full-record/WOS:000258333100025.

   

（ 1 ） 中国科学院青年创新促进会项目, 主持, 部委级, 2020-01--2023-12
（ 2 ） 外切葡聚糖酶CBH II 水解纤维素的作用机制研究和理性设计, 主持, 省级, 2021-01--2023-12
（ 3 ） 细胞内超氧化物歧化酶的致病机理研究及药物筛选方法发展, 主持, 市地级, 2021-06--2023-06
（ 4 ） 葡聚糖单加氧酶催化水解纤维素作用机制的研究, 主持, 市地级, 2021-05--2023-05
（ 5 ） 细胞内蛋白质动态性影响机制的核磁共振研究, 主持, 国家级, 2022-01--2025-12

（1）细胞内蛋白质折叠动力学的研究   2020年中科院微生物所生理与代谢工程重点实验室与青能所生物能源第二党支部“科技尖兵”交流大会   2020-10-15