上官棣华-中国科学院大学-UCAS

Research Areas

Bioanalysis

Molecular recognition
Separation and Detection of Complex Samples

Education

1992: B.S., Chinese Pharmaceutical University, Nanjing

1998: M.S., Institute of Medicinal Biotechnology, Peking Union Medical College, Beijing

2001: Ph. D, Institute of Chemistry, Chinese Academy of Sciences (ICCAS), Beijing

Experience

Work Experience

1992-1995: Pharmacist, Union Hospital, Tongji Medical University, Wuhan

2001-2003 Research Professor Assistant, ICCAS

2003-2006: Postdoc, Department of Chemistry, University of Florida, FL, USA

2007.1, Professor, ICCAS

Publications

Selected Papers

1. C. Fang, J. Zhou, X. Liu, Z. Cao and D. Shangguan*, Mercury(II)-mediated formation of imide-Hg-imide complexes. Dalton Trans., 2011, 40 (4), 899 – 903.

2. X. Yang, T. Bing, H. Mei , C. Fang, Z. Cao, D. Shangguan*, Characterization and application of a DNA aptamer binding to L-tryptophan. Analyst. 2011, 136 (3), 577 – 585

3. X. Liu, X. Cheng, T. Bing, C. Fang, and D. Shangguan*, Visual Detection of Hg2+ with High Selectivity Using Thymine Modified Gold Nanoparticles, Anal. Sci., 2010, 26(11), 1169-1172.

4. K. Sefah, D. Shangguan, X. Xiong, M. B O'Donoghue and W. Tan. Development of DNA aptamers using Cell-SELEX. Nat. Protoc. 2010, 5, 1169–1185.

5. T. Bing, X. Yang, H. Mei, Z. Cao and D. Shangguan*, Conservative Secondary Structure Motif of Streptavidin Binding Aptamers Generated by Different Laboratories, Bioorg. Med. Chem. 2010, 18, 1798–1805

6. T. Bing, X. Liu, X.. Cheng, Z. Cao and D. Shangguan*, Bifunctional Combined Aptamer for Simultaneous Separation and Detection of Thrombin. Biosens. Bioelectron., 2010, 25, 1487-1492.

7. X. Cheng, X. Liu, T. Bing, Z. Cao and D. Shangguan*, General peroxidase activity of G-quadruplex/hemin complexes and its application in ligands screening. Biochemistry, 2009, 48, 7817–7823.

8. X. Cheng, X. Liu, T. Bing, R. Zhao, S. Xiong, and D. Shangguan*, Specific DNA G-quadruplexes bind to ethanolamines. Biopolymers., 2009, 91(10),874-83.

9. Z. Zhao, L. Xu, X. Shi, W. Tan, X. Fang* and D. Shangguan*, Recognition of subtype non-small cell lung cancer by DNA aptamers selected from living cells. Analyst. 2009, 134(9):1808-14.

10. X. Liu, C. Qi, T. Bing, X. Cheng, D. Shangguan*， Highly Selective Phthalocyanine-Thymine Conjugate Sensor for Hg2+ Based on Target Induced Aggregation. Anal Chem., 2009, 81(9):3699-704.

11. X. Liu, C. Qi, T. Bing, X. Cheng, D. Shangguan*. Specific mercury(II) adsorption by thymine based sorbent. Talanta, 2009, 15;78(1):253-8. .

12. X. Cheng, T. Bing, X. Liu, D. Shangguan*. A label-free fluorescence sensor for probing the interaction of oligonucleotides with target molecules. Anal. Chim. Acta., 2009, 633(1):97-102.

13. D. Shangguan, Z.Cao, L. Meng, P. Mallikaratchy, K. Sefah, H. Wang, Y. Li and W. Tan*. Cell-specific aptamer probes for membrane protein elucidation in cancer cells. J. Proteome Res., 2008, 7(5):2133-9.

14. D. Shangguan, L. Meng, Z. C. Cao, Z. Xiao, X. Fang, Y. Li, D. Cardona, R. P. Witek, C. Liu and W. Tan*. Identification of Liver Cancer-Specific Aptamers Using Whole Live Cells. Anal. Chem., 2008, 80(3):721-728.

15. D. Shangguan, C.Cao, Y. Li and W. Tan*, Aptamers evolved from cancer cells reveal molecular differences of cancer patient samples. Clin Chem., 2007, 53(6):1153-5.

16. D. Shangguan, Z. Tang, P. Mallikaratchy, Z. Xiao, and W. Tan*. Optimization and Modifications of Aptamers Selected from Live Cancer Cell Lines, Chembiochem, 2007, 8(6):603-606.

17. D. Shangguan, Y. Li, Z. Tang, Z. C. Cao, H. W. Chen, Prabodhika Mallikaratchy, Kwame Sefah, Chaoyong James Yang, and Weihong Tan，Aptamers evolved from live cells as effective molecular probes for cancer study，Proc. Natl. Acad. Sci. USA, 2006, 103, 11838–11843.

18. Y. Dong, L. Wang, D. Shangguan*, R. Zhao, G. Liu, Improved method for the routine determination of acetylcholine and choline in brain microdialysate using a horseradish peroxidase column as the immobilized enzyme reacter, J. Chromatogr. B, 2003, 788, 193–198.

19. D. Shangguan, H. Han, R. Zhao, Y. Zhao, S. Xiong, G. Liu, New method for high-performance liquid chromatographic separation and fluorescence detection of ginsenosides, J. Chromatogr. A, 2001,910, 367-372.

20. D. Shangguan, Y. Zhao, H. Han, R. Zhao, and G. Liu, Derivatization and fluorescence detection of amino acids and peptides with 9-fluorenylmethyl chloroformate on the surface of a solid adsorbent, Anal. Chem., 2001, 73, 2054-2057

21. D. Shangguan, G. Liu. Advance in the Studies on the Metabolism of Constituents of Ginseng, ZHONGCAOYAO (Chinese Traditional and Herbal Drugs), 1999, 30(11), 865-870 (in Chinese)

22. D. Shangguan, R. Jiang, B. Li, C. Xiao and J. Wu*. Production, Isolation and Structure Elucidation of Novel Isoflavonoid Compounds K3-D4, K3-D5 and K3-D6, ZHONGGUO KANGSHENGSU ZAZHI (Chinese Journal of Antibiotics), 1999, 24(4) 254-257 (in Chinese)