Ralf  男  博导  中国科学院广州生物医药与健康研究院
电子邮件: ralf@gibh.ac.cn
通信地址: 广州市科学城开源大道190号
邮政编码: 510530

研究领域

Ralf Jauch博士是一名结构生化学家,并在基因组学、干细胞生物学和计算生物学等领域具有较强背 景,在德国和英国的大学和科学院受过严格的专业训练,对转录因子协同作用及其调控干细胞分化的分子机制,做出过原创性发现。近年来取得很多突破性成果,以第一作者或重要作者身份发表科研论文超过30篇,其多篇重要文章先后发表在Nature Cell Biol、Stem Cell、Nucleic Acid Res、EMBO等 国际知名学术刊物;    

  在Jauch博士的学术成果中,以阐明多能干细胞的早期分化原理尤为突出,得到了学术界的广泛认同。他发现Sox转录因子家族中的两个不同成员(Sox2,Sox17)均能结合控制细胞重编程的关键转录因子(Oct4),并形成二聚体复合物,但这两种复合物的生物功能完全不同。Sox2/Oct4调控体细胞重编程的发生和效率;Sox17/Oct4复合物调控多能干细胞往内胚层方向的分化。Sox2和Sox17之间只需要突变一个氨基酸,就会导致诱导体细胞重编程或多能干细胞往内胚层分化的功能互换。这个突破性发现为干细胞的应用、体外诱导肝细胞和胰腺细胞的分化,提供了充实的基础和技术支撑。 

  Jauch博士参与了研发衡量转录因子结合DNA能力的ω因子计算程序,该程序已经在转录因子研究领域被广泛应用。他以第一发明人的两项专利分别在欧洲和美国取得授权。 

  Jauch博士非常擅长独立管理多种基础型以及转化型的研究项目,其中包括转录因子的协作,基于结构的药物优化,重新设计多能性重编程因子,药物筛选和调控基因组学;


招生信息

2017年计划招收博士生3名,硕士生3名

招生专业
071010-生物化学与分子生物学
招生方向
基因组学;结构生物学;干细胞;药物研发

教育背景

2003-09--2005-07   Max-Planck-Institute, G?ttingen   PhD
2001-09--2003-07   MSc/ PhD Program ‘Molecular Biology’,G?ttingen   Graduate Student
2000-09--2001-07   University of Manchester   Visiting Student
1998-09--2000-07   Friedrich Schiller University   bachelor
1984-09--1996-07   Talschule and Angergymnasium   school education

教授课程

Workshop

专利与奖励

·              Fellowship of the Friedrich-Naumann foundation (2000-2001)

·              Qiagen stipend (2001-2002)

·       Recipient of the rewards for core professionals and talents in short supply by the Luogang government (2013)

·              100-Talent award of the Chinese Academy of Sciences/中国科学院****  (2014)

·              1st Larysa Pevny Award for Excellence in SOX research (4th SOX Research Conference, Cleveland, USA, 2014)

·              State High-end Foreign Expert/国家高端外国专家  (2015)

·              Honorary Associate Professor at the University of Hong Kong


奖励信息
(1) • 1st Larysa Pevny Award for Excellence in SOX research, 其他, 2014
专利成果
( 1 ) Mutant Sox Proteins and methods of inducing pluripotency., 发明, 2009, 第 1 作者, 专利号: 61/272,793
( 2 )  Crystallographic Structure of Mnk2 and Mnk1., 发明, 2005, 第 1 作者, 专利号: EP 050198899.3

出版信息

   
发表论文
(1) Structure and decoy-mediated inhibition of the SOX18/Prox1-DNA interaction, Nucleic Acids Res, 2016, 通讯作者
(2) Molecular basis for the genome engagement by Sox proteins, Seminars in cell & developmental biology, 2016, 通讯作者
(3) SOXE neofunctionalization and elaboration of the neural crest during chordate evolution, Scientific reports, 2016, 第 4 作者
(4) Quantitative profiling of selective Sox/POU pairing on hundreds of sequences in parallel by Coop-seq, Nucleic Acids Res, 2016, 通讯作者
(5) Changing POU dimerization preferences converts Oct6 into a pluripotency inducer, EMBO Rep, 2016, 通讯作者
(6) Reprogramming cells with synthetic proteins, Asian J Androl, 2015, 通讯作者
(7) LncRNA Dum interacts with Dnmts to regulate Dppa2 expression during myogenic differentiation and muscle regeneration, Cell Research, 2015, 第 9 作者
(8) DNA-mediated cooperativity facilitates the co-selection of cryptic enhancer sequences by SOX2 and PAX6 transcription factors, Nucleic Acids Res, 2015, 通讯作者
(9) SOXE transcription factors form selective dimers on non-compact DNA motifs through multifaceted interactions between dimerization and high-mobility group domains, Scientific reports, 2015, 通讯作者
(10) Dissecting the role of distinct OCT4-SOX2 heterodimer configurations in pluripotency, Scientific reports, 2015, 第 7 作者
(11) Structure-Based Design of Bright GFP-Based Complexes with Tunable Dimerization, Angewandte Chemie, 2015, 第 7 作者
(12) Klf4 and Klf5 differentially inhibit mesoderm and endoderm differentiation in embryonic stem cells., Nature Communications, 2014, 第 8 作者
(13) Structural basis for the SOX-dependent genomic redistribution of OCT4 in stem cell differentiation, Structure, 2014, 通讯作者
(14) Sox transcription factors require selective interactions with Oct4 and specific transactivation functions to mediate reprogramming, Stem Cells, 2013, 第 1 作者
(15) What makes a pluripotency reprogramming factor, Curr Mol Med in press, 2013, 第 1 作者
(16) Oct4 switches partnering from Sox2 to Sox17 and reinterprets the enhancer code to specify primitive endoderm, EMBO J, 2013, 第 1 作者
(17) A unique Oct4 interface is crucial for reprogramming to pluripotency. , Nature Cell Biol, 2013, 通讯作者
(18) Estimating protein-DNA binding energies from in vivo binding profiles., Nucl Acids Res,, 2013, 通讯作者
(19) Comprehensive Prediction in 78 human cell lines reveals rigidity and compactness of transcription factor dimmers. , Genome Research, 2013, 第 3 作者
(20) Differential co-factor recruitment determines STAT3s cell type-independent and cell type-specific functions, Nucleic Acids Res, 2013, 通讯作者
(21) Co-motif discovery identifies an Esrrb-Sox2-DNA ternary complex as a mediator of transcriptional differences between mouse embryonic and epiblast stem cells., Stem Cells, 2012, 通讯作者
(22) Structural Analysis and dimerization profile of the SCAN domain of the pluripotency factor Zfp206 , Nucleic Acids Res, 2012, 通讯作者
(23) A Genome-Wide Screen for Genetic Variants That Modify the Recruitment of REST to Its Target Genes, Plows Genet , 2012, 通讯作者
(24) Deciphering the Sox-Partner code by quantitative cooperativity measurements., Nucleic Acids Res, 2012, 第 4 作者
(25) Crystal structure of the Sox4 HMG/DNA complex suggests a mechanism for the positional interdependence in DNA recognition, Biochem J, 2011, 第 1 作者
(26) Conversion of Sox17 into a Pluripotency Reprogramming Factor by Re-engineering its Association with Oct4 on DNA, Stem Cells, 2011, 第 1 作者
(27) Structural basis for DNA recognition by constitutive Smad4 MH1 dimers., Nucleic Acids Res, 2011, 第 2 作者
(28) Identification of a Polyoxometalate Inhibitor of the DNA Binding Activity of Sox2, ACS Chem Biol, 2011, 通讯作者
(29) Crystal structure of the dimeric Oct6 (POU3f1) POU domain bound to palindromic MORE DNA., Proteins, 2011, 第 1 作者
(30) Structure of Smad1 MH1/DNA complex reveals distinctive rearrangements of BMP and TGF-{beta} effectors, Nucleic Acids Res, 2011, 通讯作者

科研活动

   
科研项目
( 1 ) Protein Engineering to Generate Synthetic Reprogra, 主持, 市地级, 2013-04--2017-04
( 2 ) Developing Strategies to Inhibit Transcription Fac, 主持, 市地级, 2013-04--2017-04
( 3 ) Deciphering the Enhancer Code and Transcription Fa, 主持, 市地级, 2013-04--2017-04
( 4 ) Generation of Genetically Modified Mesenchymal Ste, 主持, 国家级, 2013-06--2016-06
( 5 ) 通过工程化改造Oct4研究体细胞重编程过程的基因组调控机制, 主持, 国家级, 2015-01--2018-12
( 6 ) 中科院****, 主持, 部委级, 2015-01--2017-12
( 7 ) 鉴别和研究OCT4在重编程和基因增强调控中的互作蛋白体, 主持, 国家级, 2016-01--2018-12
( 8 ) 研究致癌SOX突变体的基因调控机制, 主持, 省级, 2016-01--2017-12
( 9 ) 非编码RNA介导的染色质高级结构动态变化对多能干细胞命运决定的调控功能及分子机制,, 主持, 国家级, 2016-01--2017-12
参与会议