General

Guohui Pan Ph.D. Principle Investigator

Institute of Microbiology, Chinese Academy of Sciences

Email: panguohui@im.ac.cn

NO. 1 West Beichen Road, Chaoyang District, Beijing 100101, China


Research Areas

Microbial natural product genome-based discovery, biosynthesis and synthetic biology.

Education

Sep. 2008 – Jan. 2014, Ph.D.

Biochemistry and Molecular Biology, Institute of Microbiology, Chinese Academy of Sciences (IMCAS) & University of the Chinese Academy Sciences, Beijing, China.

Sep. 2004 – Sep. 2008, B.S.

Biological Engineering, School of Chemical & Enviromental Engineering, China University of Mining and Technology-Beijing (CUMTB), Beijing, China.

Experience

   
Work Experience

Aug. 2019 – present, Principle investigator

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences

Feb. 2014 – Aug. 2019, Postdoctoral Research Associate

Department of Chemistry, The Scripps Research Institute – Florida, Jupiter, Florida, USA.

Publications

   
Papers

# Co-first authors

1. Kalkreuter E, Pan G, Cepeda AJ, Shen B*. (2020) Targeting bacterial genomes for natural product discovery. Trends Pharmacol Sci. 41:13-26.

2. Steele AD, Kalkreuter E, Pan G, Meng S, Shen B*. (2019) "Hybrid Peptide-Polyketide Natural Product Biosynthesis" in Comprehensive Natural Products: Chemistry and Biology, Vol I, Elsevier, in press.

3. McAlpine et al. (2019) The value of universally available raw NMR data for transparency, reproducibility, and integrity in natural product research. Nat Prod Rep. 36, 35-107.

4. Xu Z#, Pan G#, Zhou H, Shen B*. (2018) Discovery and characterization of 1-aminocyclopropane-1-carboxylic acid synthase of bacterial origin. J Am Chem Soc. 140, 16957-61. (Hot off the press)

5. Kwong T#, Ma M#, Pan G, Hindra, Yang D, Yang C, Lohman JR, Rudolf JD, Cleveland JL, Shen B*. (2018) P450-catalyzed tailoring steps in leinamycin biosynthesis featuring regio- and stereoselective hydroxylations and substrate promiscuities. Biochemistry. 57, 5005–13.

6. Pan G#, Xu Z#, Guo Z, Hindra, Ma M, Yang D, Zhou H, Gansemans Y, Zhu X, Huang Y, Zhao LX, Jiang Y, Cheng J, Van Nieuwerburgh F, Suh JW, Duan Y, Shen B*. (2017) Discovery of the leinamycin family of natural products by mining actinobacterial genomes. Proc Natl Acad Sci U S A. 114, E11131-40. (Highlighted by F1000)

7. Zhang B#, Xu Z#, Teng Q#, Pan G, Ma M, Shen B*. (2017) A Long-range effecting dehydratase domain as the missing link for C17-dehydration in iso-migrastatin biosynthesis. Angew. Chem. Int. Ed. 56, 7247-51.

8. Li L#, Pan G#, Zhu X, Fan K, Gao W, Ai G, Ren J, Shi M, Olano C, Salas J, Yang K*. (2017) Engineered jadomycin analogues with altered sugar moieties revealing JadS as a substrate flexible O-glycosyltransferase. Appl Microbiol Biotechnol. 101:5291-300.

9. Pan G#, Gao X#, Fan K#, Liu J, Meng B, Gao J, Wang B, Zhang C, Han H, Ai G, Chen Y, Wu D*, Liu ZJ*, Yang K*. (2017) Structure and function of a C-C bond cleaving oxygenase in atypical angucycline biosynthesis. ACS Chem Biol. 12, 142-52.

10. Guo Z, Pan G, Xu Z, Yang D, Hindra, Zhu X, Huang Y, Zhao LX, Jiang Y, Duan Y, Shen B*. (2017) New isofuranonaphthoquinones and isoindolequinones from Streptomyces sp. CB01883. J Antibiot. 70, 414-22.

11. Huang Y#, Yang D#, Pan G, Tang GL, Shen B*. (2016) Characterization of LnmO as a pathway-specific Crp/Fnr-type positive regulator for leinamycin biosynthesis in Streptomyces atroolivaceus and its application for titer improvement. Appl Microbiol Biotechnol. 100, 10555-62.

12. Huang Y, Tang GL, Pan G, Chang CY, Shen B. (2016) Characterization of the Ketosynthase and Acyl Carrier Protein Domains at the LnmI Nonribosomal Peptide Synthetase-Polyketide Synthase Interface for Leinamycin Biosynthesis. Org Lett. 18, 4288-91. 

13. Zhang B#, Yang D#, Yan Y, Pan G, Xiang W*, Shen B*. (2016) Overproduction of lactimidomycin by cross-overexpression of genes encoding Streptomyces antibiotic regulatory proteins. Appl Microbiol Biotechnol. 100, 2267-77.

14. Medema MH et al. (2015) Minimum information about a biosynthetic gene cluster. Nat Chem Biol. 11, 625-31. (Highlighted by F1000)

15. Wang B#, Ren J#, Li L, Guo F, Pan G, Ai G, Aigle B, Fan K*, Yang K*. (2015) Kinamycin biosynthesis employs a conserved pair of oxidases for B-ring contraction. Chem Commun. 51, 8845-8.

16. Liu Y, Ryu H, Ge B, Pan G, Sun L, Park K, Zhang K*. (2014) Improvement of wuyiencin biosynthesis in Streptomyces wuyiensis CK-15 by identification of a key regulator, WysR. J Microbiol Biotechnol. 24, 1644-53.

17. Wang W#, Ji J#, Li X, Wang J, Li S, Pan G, Fan K, Yang K*. (2014) Angucyclines as signals modulate the behaviors of Streptomyces coelicolorProc Natl Acad Sci U S A. 111, 5688-93.

18. Zhang Y#, Pan G#, Zou Z, Fan K, Yang K*, Tan H*. (2013) JadR*-mediated feed-forward regulation of cofactor supply in jadomycin biosynthesis. Mol Microbiol. 90, 884-97.

19. Fan K#, Pan G#, Peng X, Zheng J, Gao W, Wang J, Wang W, Li Y, Yang K*. (2012) Identification of JadG as the B ring opening oxygenase catalyzing the oxidative C-C bond cleavage reaction in jadomycin biosynthesis. Chem Biol. 19, 1381-90.


Research Interests

Microbial natural product genome-based discovery, biosynthesis and synthetic biology.

Students

已指导学生

袁芳  博士研究生  071010-生物化学与分子生物学  

张倩  博士研究生  071010-生物化学与分子生物学  

现指导学生

李栋  博士研究生  100705-微生物与生化药学  

向丽军  硕士研究生  078005-微生物与生化药学  

刘耀  博士研究生  100705-微生物与生化药学  

陈雨佳  硕士研究生  086000-生物与医药  

沈世杰  硕士研究生  078005-微生物与生化药学  

胡会涛  博士研究生  100705-微生物与生化药学