General

Chunbo Lou

Professor of Shenzhen Institute of Synthetic Biology

SIAT,CAS

E-mail: cb.lou@siat.ac.cn


Research Areas

Mammalian genetic circuit; gene therapy, vaccine design, predictive models, synthetic biology, anti-tuberculosis drug

Education

EDUCATION

B.S. Physics, Wuhan University, 1999.9-2003.7.

Ph.D. Physics, Peking University, 2003.9-2009.1.


Experience

PROFESSIONAL EMPLOYMENT

2019.9-now: Professor, Shenzhen Institute of Advanced Technology, Chinese Academy of Science.

2013.3-2019.6: Professor, Institute of Microbiology, Chinese Academy of Science.

2011.8-2013.1: Postdoctoral associate, Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute of Technology.

2009.8-2011.8: Postdoctoral Scholar, Department of Pharmaceutical Chemistry, University of California, San Francisco.

2003.9-2009.7: Research Assistant, Center for Theoretical Biology, School of Physics, Peking University


Honors & Distinctions


Best Poster and presenter Award, Synthetic Biology Engineering Research Center (SynBERC) conference, 2011

Grand Prize, iGEM competition, Mentor and Project Designer of PKU Team, 2007

Zhong Shengbiao Scholarship, Peking University, 2007




Publications

   
Papers

1.        Miao, C., Zhao, H., Qian, L., & Lou, C. (2019). Systematically investigating the key features of the DNase deactivated Cpf1 for tunable transcription regulation in prokaryotic cells. Synthetic and Systems Biotechnology, 4(1), 1-9.

2.        Zheng, Y., Meng, F., Zhu, Z., Wei, W., Sun, Z., Chen, J., Lou, C*. & Chen, G. Q*. (2019). A tight cold-inducible switch built by coupling thermosensitive transcriptional and proteolytic regulatory parts. Nucleic acids research, 47(21), e137-e137.

3.        Li, T., Dong, Y., Zhang, X., Ji, X., Luo, C., Lou, C.,  & Ouyang, Q*. (2018). Engineering of a genetic circuit with regulatable multistability. Integrative Biology, 10(8), 474-482.

4.        Hou, J., Zeng, W., Zong, Y., Chen, Z., Miao, C., Wang, B., & Lou, C*. (2018). Engineering the Ultrasensitive Transcription Factors by Fusing a Modular Oligomerization Domain. ACS synthetic biology, 7(5), 1188-1194.

5.        Tang Q, Lou C*, Liu SJ*. Construction of an easy-to-use CRISPR-Cas9 system by patching a newly designed EXIT circuit. Journal of biological engineering, 2017, 11(1): 32.

6.        Zong, Y., Zhang, H. M., Lyu, C., Ji, X., Hou, J., Guo, X., Ouyang Q*, & Lou, C*. (2017). Insulated transcriptional elements enable precise design of genetic circuits. Nature communications, 8(1), 52.

7.        Zhang Y., Qian L., Wei W., …, Ouyang Q. *, & Lou C*. (2016). Paired design of dCas9 as a systematic platform for the detection of featured nucleic acid sequences in pathogenic strains. ACS synthetic biology. 6(2), 211-216

8.        Li, T., Ye, J., Shen, R., Zong, Y., Zhao, X., Lou, C. *, & Chen, G. Q. * (2016). Semi-rational approach for ultra-high poly (3-hydroxybutyrate) accumulation in Escherichia coli by combining one-step library construction and high-throughput screeningACS synthetic biology. 5(11), 1308-1317

9.        Zhang, H. M., Chen, S., Shi, H., Ji, W., Zong, Y., Ouyang, Q. *, & Lou, C*. (2015). Measurements of Gene Expression at Steady State Improve the Predictability of Part Assembly. ACS synthetic biology, 5(3), 269-273.

10.    Zhang S, Zhao X, Tao Y* and Lou C*. (2015).A novel approach for metabolic pathway optimization: oligo-liker mediated assembly (OLMA) method. Journal of Biological Engineering, 9(1):1-10

11.    Pei Du, Chensi Miao, Qiuli Lou, Zefeng Wang*, and Chunbo Lou* (2015). Engineering translational activators with CRISPR-Cas system. ACS synthetic biology. 5(1), 74-80

12.    Chaoxian Bai#, Yang Zhang#, Xuejin Zhao#, Yiling Hu, Sihai Xiang, Jin Miao, Lixin Zhang* and Chunbo Lou* (2015)  Exploiting a precise design of universal synthetic modular regulatory elements to unlock the microbial natural products in Streptomyces. PNAS, 112(39): 12181-12186.

13.    Wenjun Jiang, Xuejin Zhao, Tslil Gabrieli, Chunbo Lou*, Yuval Ebenstein*, and Ting Zhu *. (2015). Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters. Nature communs,6.

14.    Tingting Li#, Teng Li#, Weiyue Ji, Qiuyue Wang, Haoqian Zhang, Guo-Qiang Chen, Chunbo Lou* and Qi Ouyang*. (2015) Engineering of core promoter regions enables the construction of constitutive and inducible promoters in Halomonas sp. Biotechnol. J.  DOI: 10.1002/biot.201400828

15.    Lou CB, Stanton B, Chen YJ Munsky B, Voigt CA. 2012. Ribozyme-based “insulator parts” buffer synthetic circuits from genetic context. Nature Biotechnol. 30(11), 1137-1142.

16.    Moon TS, Lou CB, Stanton B, Tamsir A, Voigt CA. 2012. Genetic programs constructed from layered logic gates in single cells. Nature. 491(7423), 249-253.

17.    Lou CB, Liu XL, et al. 2010. Synthesizing a novel genetic sequential logic circuit. Mol. Syst. Biol. 6:350. 


Patents

1. Synthetic Biology Tools, Chunbo Lou, Tae Seok Moon, Virgil Rhodius, Brynne Stanton, Alvin Tamsir, Karsten Temme and Christopher Voigt, U.S. Patent Application No. 13/489,205.PCT/US2012/040939; WO 2012/170436.A1

2. Methods and Kits for Fragmenting DNA, Ting F. Zhu, Yuval Ebenstein, Chunbo Lou, Wenjun Jiang, Xuejin Zhao, Tslil Gabrieli, U.S. Patent Application No. 62/156,417;  PCT/IL2016/0503394.; WO 2016/178207.A1

3. Novel Oligo-linker-mediated DNA Assembly Method and Applications Thereof.  Chunb Lou, Yong Tao, Xuejin Zhao, Shasha Zhang, Lihua Zhang, Chunhua Zhai, Zhenyu Liu, U.S. Patent Application US2016/0340670. A1

4. 基于CRISPR/Cas9和酿酒酵母细胞内源的同源重组的分子克隆方法,娄春波,赵学金, 发明专利CN 105624146,专利号ZL 2015 1 0282184.X

5. 构建生产或高产目的基因产物重组菌的方法及构建的重组菌与应用, 张立新;胡逸灵;娄春波;白超弦;苗靳;向四海;黄佩;, 发明专利CN105176899A

核酸的检测方法娄春波;欧阳颀;张益豪;魏伟佳; 发明专利CN105177110A

6.  阻遏蛋白、调控元件组和基因表达调控系统及其构建方法  娄春波;侯君然; 发明专利CN107344962A

7. 用于处理重金属污染的微生物细胞和蛋白、相应的处理方法和试剂盒 娄春波;杜沛;魏伟佳;王瑞沙;姜浩;张浩千发明专利CN107916243A

8. 一种可编程的多位点特异性的转录抑制系统及其应用 苗晨思;赵会伟;关莹;陈泽华;娄春波; 发明专利CN107312790A